TY  - JOUR
AU  - Hasegawa, Nonno
AU  - Techer, Maeva A.
AU  - Adjlane, Noureddine
AU  - Sabah al-Hissnawi, Muntasser
AU  - Antúnez, Karina
AU  - Beaurepaire, Alexis
AU  - Christmon, Krisztina
AU  - Delatte, Helene
AU  - Dukku, Usman H.
AU  - Eliash, Nurit
AU  - El-Niweiri, Mogbel A. A
AU  - Esnault, Olivier
AU  - Evans, Jay D.
AU  - Haddad, Nizar J.
AU  - Locke, Barbara
AU  - Muñoz, Irene
AU  - Noël, Grégoire
AU  - Panziera, Delphine
AU  - Roberts, John M. K.
AU  - De la Rúa, Pilar
AU  - Shebl, Mohamed A.
AU  - Stanimirović, Zoran
AU  - Rasmussen, David A.
AU  - Mikheyev, Alexander S.
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3146
AB  - Novel transmission routes can allow infectious diseases to spread, often with devastating consequences. Ectoparasitic varroa mites vector a diversity of RNA viruses, having switched hosts from the eastern to western honey bees (Apis cerana to Apis mellifera). They provide an opportunity to explore how novel transmission routes shape disease epidemiology. As the principal driver of the spread of deformed wing viruses (mainly DWV-A and DWV-B), varroa infestation has also driven global honey bee health declines. The more virulent DWV-B strain has been replacing the original DWV-A strain in many regions over the past two decades. Yet, how these viruses originated and spread remains poorly understood. Here, we use a phylogeographic analysis based on whole-genome data to reconstruct the origins and demography of DWV spread. We found that, rather than reemerging in western honey bees after varroa switched hosts, as suggested by previous work, DWV-A most likely originated in East Asia and spread in the mid-20th century. It also showed a massive population size expansion following the varroa host switch. By contrast, DWV-B was most likely acquired more recently from a source outside East Asia and appears absent from the original varroa host. These results highlight the dynamic nature of viral adaptation, whereby a vector's host switch can give rise to competing and increasingly virulent disease pandemics. The evolutionary novelty and rapid global spread of these host-virus interactions, together with observed spillover into other species, illustrate how increasing globalization poses urgent threats to biodiversity and food security.
PB  - National Academy of Sciences
T2  - Proceedings of the National Academy of Sciences (PNAS)
T1  - Evolutionarily diverse origins of deformed wing viruses in western honey bees
VL  - 120
IS  - 26
SP  - 2301258120
DO  - 10.1073/pnas.2301258120
ER  - 

@article{
author = "Hasegawa, Nonno and Techer, Maeva A. and Adjlane, Noureddine and Sabah al-Hissnawi, Muntasser and Antúnez, Karina and Beaurepaire, Alexis and Christmon, Krisztina and Delatte, Helene and Dukku, Usman H. and Eliash, Nurit and El-Niweiri, Mogbel A. A and Esnault, Olivier and Evans, Jay D. and Haddad, Nizar J. and Locke, Barbara and Muñoz, Irene and Noël, Grégoire and Panziera, Delphine and Roberts, John M. K. and De la Rúa, Pilar and Shebl, Mohamed A. and Stanimirović, Zoran and Rasmussen, David A. and Mikheyev, Alexander S.",
year = "2023",
abstract = "Novel transmission routes can allow infectious diseases to spread, often with devastating consequences. Ectoparasitic varroa mites vector a diversity of RNA viruses, having switched hosts from the eastern to western honey bees (Apis cerana to Apis mellifera). They provide an opportunity to explore how novel transmission routes shape disease epidemiology. As the principal driver of the spread of deformed wing viruses (mainly DWV-A and DWV-B), varroa infestation has also driven global honey bee health declines. The more virulent DWV-B strain has been replacing the original DWV-A strain in many regions over the past two decades. Yet, how these viruses originated and spread remains poorly understood. Here, we use a phylogeographic analysis based on whole-genome data to reconstruct the origins and demography of DWV spread. We found that, rather than reemerging in western honey bees after varroa switched hosts, as suggested by previous work, DWV-A most likely originated in East Asia and spread in the mid-20th century. It also showed a massive population size expansion following the varroa host switch. By contrast, DWV-B was most likely acquired more recently from a source outside East Asia and appears absent from the original varroa host. These results highlight the dynamic nature of viral adaptation, whereby a vector's host switch can give rise to competing and increasingly virulent disease pandemics. The evolutionary novelty and rapid global spread of these host-virus interactions, together with observed spillover into other species, illustrate how increasing globalization poses urgent threats to biodiversity and food security.",
publisher = "National Academy of Sciences",
journal = "Proceedings of the National Academy of Sciences (PNAS)",
title = "Evolutionarily diverse origins of deformed wing viruses in western honey bees",
volume = "120",
number = "26",
pages = "2301258120",
doi = "10.1073/pnas.2301258120"
}

Hasegawa, N., Techer, M. A., Adjlane, N., Sabah al-Hissnawi, M., Antúnez, K., Beaurepaire, A., Christmon, K., Delatte, H., Dukku, U. H., Eliash, N., El-Niweiri, M. A. A., Esnault, O., Evans, J. D., Haddad, N. J., Locke, B., Muñoz, I., Noël, G., Panziera, D., Roberts, J. M. K., De la Rúa, P., Shebl, M. A., Stanimirović, Z., Rasmussen, D. A.,& Mikheyev, A. S.. (2023). Evolutionarily diverse origins of deformed wing viruses in western honey bees. in Proceedings of the National Academy of Sciences (PNAS)
National Academy of Sciences., 120(26), 2301258120.
https://doi.org/10.1073/pnas.2301258120

Hasegawa N, Techer MA, Adjlane N, Sabah al-Hissnawi M, Antúnez K, Beaurepaire A, Christmon K, Delatte H, Dukku UH, Eliash N, El-Niweiri MAA, Esnault O, Evans JD, Haddad NJ, Locke B, Muñoz I, Noël G, Panziera D, Roberts JMK, De la Rúa P, Shebl MA, Stanimirović Z, Rasmussen DA, Mikheyev AS. Evolutionarily diverse origins of deformed wing viruses in western honey bees. in Proceedings of the National Academy of Sciences (PNAS). 2023;120(26):2301258120.
doi:10.1073/pnas.2301258120 .

Hasegawa, Nonno, Techer, Maeva A., Adjlane, Noureddine, Sabah al-Hissnawi, Muntasser, Antúnez, Karina, Beaurepaire, Alexis, Christmon, Krisztina, Delatte, Helene, Dukku, Usman H., Eliash, Nurit, El-Niweiri, Mogbel A. A, Esnault, Olivier, Evans, Jay D., Haddad, Nizar J., Locke, Barbara, Muñoz, Irene, Noël, Grégoire, Panziera, Delphine, Roberts, John M. K., De la Rúa, Pilar, Shebl, Mohamed A., Stanimirović, Zoran, Rasmussen, David A., Mikheyev, Alexander S., "Evolutionarily diverse origins of deformed wing viruses in western honey bees" in Proceedings of the National Academy of Sciences (PNAS), 120, no. 26 (2023):2301258120,
https://doi.org/10.1073/pnas.2301258120 . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Schwarz, Ryan S.
AU  - Vejnović, Branislav
AU  - Evans, Jay D.
AU  - Irwin, Rebecca E.
AU  - Glavinić, Uroš
AU  - Stanimirović, Zoran
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1391
AB  - In this study, honey bees collected in Serbia over 9 consecutive years (2007-2015) were retrospectively surveyed to determine the prevalence of eukaryotic gut parasites by molecular screening of archival DNA samples. We developed species-specific primers for PCR to detect the two known honey bee trypanosomatid species, Crithidia mellificae and the recently described Lotmaria passim. These primers were validated for target specificity under single and mixed-species conditions as well as against the bumblebee trypanosomatid Crithidia bombi. Infections by Nosema apis and Nosema ceranae (Microsporidia) were also determined using PCR. Samples from 162 colonies (18 from each year) originating from 57 different localities were surveyed. L. passim was detected in every year with an overall frequency of 62.3% and annual frequencies ranging from 38.9% to 83.3%. This provides the earliest confirmed record to date for L. passim and the first report of this species in Serbia. N. ceranae was ubiquitous, occurring in every year and at 95.7% overall frequency, ranging annually from 83.3% to 100%. The majority of colonies (60.5%) were co-infected with L. passim and N. ceranae, but colony infections by each species were statistically independent of one another over the nine years. Although C. mellificae and N. apis have both been reported recently at low frequency in Europe, neither of these species was detected in Serbia. These results support the hypothesis that L. passim has predominated over C mellificae in A. mellifera during the past decade.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Journal of Invertebrate Pathology
T1  - Species-specific diagnostics of Apis mellifera trypanosomatids: A nine-year survey (2007-2015) for trypanosomatids and microsporidians in Serbian honey bees
VL  - 139
SP  - 6
EP  - 11
DO  - 10.1016/j.jip.2016.07.001
ER  - 

@article{
author = "Stevanović, Jevrosima and Schwarz, Ryan S. and Vejnović, Branislav and Evans, Jay D. and Irwin, Rebecca E. and Glavinić, Uroš and Stanimirović, Zoran",
year = "2016",
abstract = "In this study, honey bees collected in Serbia over 9 consecutive years (2007-2015) were retrospectively surveyed to determine the prevalence of eukaryotic gut parasites by molecular screening of archival DNA samples. We developed species-specific primers for PCR to detect the two known honey bee trypanosomatid species, Crithidia mellificae and the recently described Lotmaria passim. These primers were validated for target specificity under single and mixed-species conditions as well as against the bumblebee trypanosomatid Crithidia bombi. Infections by Nosema apis and Nosema ceranae (Microsporidia) were also determined using PCR. Samples from 162 colonies (18 from each year) originating from 57 different localities were surveyed. L. passim was detected in every year with an overall frequency of 62.3% and annual frequencies ranging from 38.9% to 83.3%. This provides the earliest confirmed record to date for L. passim and the first report of this species in Serbia. N. ceranae was ubiquitous, occurring in every year and at 95.7% overall frequency, ranging annually from 83.3% to 100%. The majority of colonies (60.5%) were co-infected with L. passim and N. ceranae, but colony infections by each species were statistically independent of one another over the nine years. Although C. mellificae and N. apis have both been reported recently at low frequency in Europe, neither of these species was detected in Serbia. These results support the hypothesis that L. passim has predominated over C mellificae in A. mellifera during the past decade.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Journal of Invertebrate Pathology",
title = "Species-specific diagnostics of Apis mellifera trypanosomatids: A nine-year survey (2007-2015) for trypanosomatids and microsporidians in Serbian honey bees",
volume = "139",
pages = "6-11",
doi = "10.1016/j.jip.2016.07.001"
}

Stevanović, J., Schwarz, R. S., Vejnović, B., Evans, J. D., Irwin, R. E., Glavinić, U.,& Stanimirović, Z.. (2016). Species-specific diagnostics of Apis mellifera trypanosomatids: A nine-year survey (2007-2015) for trypanosomatids and microsporidians in Serbian honey bees. in Journal of Invertebrate Pathology
Academic Press Inc Elsevier Science, San Diego., 139, 6-11.
https://doi.org/10.1016/j.jip.2016.07.001

Stevanović J, Schwarz RS, Vejnović B, Evans JD, Irwin RE, Glavinić U, Stanimirović Z. Species-specific diagnostics of Apis mellifera trypanosomatids: A nine-year survey (2007-2015) for trypanosomatids and microsporidians in Serbian honey bees. in Journal of Invertebrate Pathology. 2016;139:6-11.
doi:10.1016/j.jip.2016.07.001 .

Stevanović, Jevrosima, Schwarz, Ryan S., Vejnović, Branislav, Evans, Jay D., Irwin, Rebecca E., Glavinić, Uroš, Stanimirović, Zoran, "Species-specific diagnostics of Apis mellifera trypanosomatids: A nine-year survey (2007-2015) for trypanosomatids and microsporidians in Serbian honey bees" in Journal of Invertebrate Pathology, 139 (2016):6-11,
https://doi.org/10.1016/j.jip.2016.07.001 . .