TY  - JOUR
AU  - Milojević, Lazar
AU  - Velebit, Branko
AU  - Janković, Vesna
AU  - Mitrović, Radmila
AU  - Betić, Nikola
AU  - Simunović, Sara
AU  - Dimitrijević, Mirjana
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3705
AB  - The goal of this study conducted in Serbia was to detect HEV in pig liver samples from
slaughterhouses, retail outlets, and environmental swabs over the course of a year. All positive
HEV samples were measured and expressed as HEV gene copy numbers per gram of sample, and a
representative number of samples were sequenced using the Sanger approach. A total of 45 HEVpositive samples were re-amplified using nested RT-PCR employing CODEHOP primers targeting
ORF2 (493 nucleotides). The average prevalence of the HEV genotype 3 in all pig liver samples from
the slaughterhouses was 29%, while HEV prevalence was 44% in liver samples from animals younger
than 3 months. HEV RNA was found in thirteen out of sixty (22%) environmental swab samples
that were taken from different surfaces along the slaughter line. Our findings confirmed seasonal
patterns in HEV prevalence, with two picks (summer and winter periods) during the one-year
examination. Among HEV-positive samples, the average viral particles for all positive liver samples
was 4.41 ± 1.69 log10 genome copies per gram. Phylogenetic analysis revealed the majority of HEV
strains (43/45) from Serbia were grouped in the HEV-3a subtype, while two strains were classified
into the HEV-3c subtype, and one strain could not be classified into any of the HEV-3 subtypes.
PB  - MDPI
T2  - Animals
T1  - Prevalence, genetic diversity, and quantification of the RNA genome of the hepatitis E virus in slaughtered pigs in Serbia
VL  - 14
IS  - 4
SP  - 586
DO  - 10.3390/ani14040586
ER  - 

@article{
author = "Milojević, Lazar and Velebit, Branko and Janković, Vesna and Mitrović, Radmila and Betić, Nikola and Simunović, Sara and Dimitrijević, Mirjana",
year = "2024",
abstract = "The goal of this study conducted in Serbia was to detect HEV in pig liver samples from
slaughterhouses, retail outlets, and environmental swabs over the course of a year. All positive
HEV samples were measured and expressed as HEV gene copy numbers per gram of sample, and a
representative number of samples were sequenced using the Sanger approach. A total of 45 HEVpositive samples were re-amplified using nested RT-PCR employing CODEHOP primers targeting
ORF2 (493 nucleotides). The average prevalence of the HEV genotype 3 in all pig liver samples from
the slaughterhouses was 29%, while HEV prevalence was 44% in liver samples from animals younger
than 3 months. HEV RNA was found in thirteen out of sixty (22%) environmental swab samples
that were taken from different surfaces along the slaughter line. Our findings confirmed seasonal
patterns in HEV prevalence, with two picks (summer and winter periods) during the one-year
examination. Among HEV-positive samples, the average viral particles for all positive liver samples
was 4.41 ± 1.69 log10 genome copies per gram. Phylogenetic analysis revealed the majority of HEV
strains (43/45) from Serbia were grouped in the HEV-3a subtype, while two strains were classified
into the HEV-3c subtype, and one strain could not be classified into any of the HEV-3 subtypes.",
publisher = "MDPI",
journal = "Animals",
title = "Prevalence, genetic diversity, and quantification of the RNA genome of the hepatitis E virus in slaughtered pigs in Serbia",
volume = "14",
number = "4",
pages = "586",
doi = "10.3390/ani14040586"
}

Milojević, L., Velebit, B., Janković, V., Mitrović, R., Betić, N., Simunović, S.,& Dimitrijević, M.. (2024). Prevalence, genetic diversity, and quantification of the RNA genome of the hepatitis E virus in slaughtered pigs in Serbia. in Animals
MDPI., 14(4), 586.
https://doi.org/10.3390/ani14040586

Milojević L, Velebit B, Janković V, Mitrović R, Betić N, Simunović S, Dimitrijević M. Prevalence, genetic diversity, and quantification of the RNA genome of the hepatitis E virus in slaughtered pigs in Serbia. in Animals. 2024;14(4):586.
doi:10.3390/ani14040586 .

Milojević, Lazar, Velebit, Branko, Janković, Vesna, Mitrović, Radmila, Betić, Nikola, Simunović, Sara, Dimitrijević, Mirjana, "Prevalence, genetic diversity, and quantification of the RNA genome of the hepatitis E virus in slaughtered pigs in Serbia" in Animals, 14, no. 4 (2024):586,
https://doi.org/10.3390/ani14040586 . .

TY  - JOUR
AU  - Krstić, Miljana
AU  - Đorđević, Vesna
AU  - Ćirić, Jelena
AU  - Baltić, Tatjana
AU  - Bajčić, Aleksandar
AU  - Simunović, Sara
AU  - Perić, Dejan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3769
AB  - of fattening broilers involves raising broilers on farms with a capacity of 5,000 to 50,000
units or more at a density of 0.06 m2 per bird. Modern poultry farms are constructed
with the task of reducing heat loss, i.e. improving energy efficiency, which very often
in combination with reduced ventilation can lead to increased levels of ammonia (NH3),
carbon dioxide (CO2) and other air pollutants, and thus adversely affect animal health
and productivity. The speed of gas emissions is influenced by many factors, such as the
composition of feed and the efficiency of feed use (conversion), the quality of the litter
and the microclimatic conditions on the farm. The litter on intensive poultry farms usually
contains 4 to 6% of nitrogen, most of which is in NH3 or NH4
+ form. The mixture
of litter and manure is a storage of nitrogen which is released in the form of ammonia
under appropriate conditions. On the other hand, the main source of carbon dioxide in
livestock is the product of animal respiration, so there is a connection between the levels
of animal metabolism and CO2 production on farms. The production of carbon dioxide in
birds is proportional to their metabolic heat production, and thus to the metabolic body
mass of the bird, which is affected by temperature and activity. The aim of the study
was to examine the effect of a nutritional supplement, Eubiotic, added to broiler feed on
the NH3 and CO2 emissions in a broiler farm. The values of NH3 and CO2 emissions in
the facility for breeding fattening broilers that received Eubiotic in feed were numerically
lower, which can be explained by better digestibility of basic nutrients, primarily
proteins, present in feed.
PB  - Institute of Meat Hygiene and Technology
T2  - Meat Technology
T1  - Nutritional strategies to reduce ammonia and carbon dioxide production in intensive livestock production
VL  - 64
IS  - 2
SP  - 387
EP  - 391
DO  - 10.18485/meattech.2023.64.2.74
ER  - 

@article{
author = "Krstić, Miljana and Đorđević, Vesna and Ćirić, Jelena and Baltić, Tatjana and Bajčić, Aleksandar and Simunović, Sara and Perić, Dejan",
year = "2023",
abstract = "of fattening broilers involves raising broilers on farms with a capacity of 5,000 to 50,000
units or more at a density of 0.06 m2 per bird. Modern poultry farms are constructed
with the task of reducing heat loss, i.e. improving energy efficiency, which very often
in combination with reduced ventilation can lead to increased levels of ammonia (NH3),
carbon dioxide (CO2) and other air pollutants, and thus adversely affect animal health
and productivity. The speed of gas emissions is influenced by many factors, such as the
composition of feed and the efficiency of feed use (conversion), the quality of the litter
and the microclimatic conditions on the farm. The litter on intensive poultry farms usually
contains 4 to 6% of nitrogen, most of which is in NH3 or NH4
+ form. The mixture
of litter and manure is a storage of nitrogen which is released in the form of ammonia
under appropriate conditions. On the other hand, the main source of carbon dioxide in
livestock is the product of animal respiration, so there is a connection between the levels
of animal metabolism and CO2 production on farms. The production of carbon dioxide in
birds is proportional to their metabolic heat production, and thus to the metabolic body
mass of the bird, which is affected by temperature and activity. The aim of the study
was to examine the effect of a nutritional supplement, Eubiotic, added to broiler feed on
the NH3 and CO2 emissions in a broiler farm. The values of NH3 and CO2 emissions in
the facility for breeding fattening broilers that received Eubiotic in feed were numerically
lower, which can be explained by better digestibility of basic nutrients, primarily
proteins, present in feed.",
publisher = "Institute of Meat Hygiene and Technology",
journal = "Meat Technology",
title = "Nutritional strategies to reduce ammonia and carbon dioxide production in intensive livestock production",
volume = "64",
number = "2",
pages = "387-391",
doi = "10.18485/meattech.2023.64.2.74"
}

Krstić, M., Đorđević, V., Ćirić, J., Baltić, T., Bajčić, A., Simunović, S.,& Perić, D.. (2023). Nutritional strategies to reduce ammonia and carbon dioxide production in intensive livestock production. in Meat Technology
Institute of Meat Hygiene and Technology., 64(2), 387-391.
https://doi.org/10.18485/meattech.2023.64.2.74

Krstić M, Đorđević V, Ćirić J, Baltić T, Bajčić A, Simunović S, Perić D. Nutritional strategies to reduce ammonia and carbon dioxide production in intensive livestock production. in Meat Technology. 2023;64(2):387-391.
doi:10.18485/meattech.2023.64.2.74 .

Krstić, Miljana, Đorđević, Vesna, Ćirić, Jelena, Baltić, Tatjana, Bajčić, Aleksandar, Simunović, Sara, Perić, Dejan, "Nutritional strategies to reduce ammonia and carbon dioxide production in intensive livestock production" in Meat Technology, 64, no. 2 (2023):387-391,
https://doi.org/10.18485/meattech.2023.64.2.74 . .

TY  - JOUR
AU  - Rašeta, Mladen
AU  - Branković-Lazić, Ivana
AU  - Mrdović, Boris
AU  - Simunović, Stefan
AU  - Simunović, Sara
AU  - Jovanović, Jelena
AU  - Becskei, Zsolt
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3493
AB  - The aim of this work is to ensure the safety of finely chopped cooked sausages stuffed in a cellulose
casing, diameter 21 mm. Safety is ensured by thermal treatment of the level of pasteurization
in a chamber under a defined thermal treatment program. By monitoring the temperature
in the product’s thermal center at different control positions in the chamber, temperatures
of 75.26℃–77.11°C were determined with a thermocouple. In doing so, the products spent
8–9 minutes at a temperature higher than 70°C, and 18–20 minutes at a temperature higher
than 55°C. Considering that it is a small diameter product, the heat intensively penetrates to
the thermal center and thus ensures the safety of the product. However, due to the relatively
short time of the heat treatment, the pasteurization values (pv) at the control points were in the
range of 18.3–26 minutes. Therefore, we conclude that ensuring the safety of products with
a narrower diameter is achieved only by controlling the temperature in the thermal center of
the product, and not with pv values. Considering that this sausage is a perishable product,
it is necessary to provide a proper cold chain (0–4℃) after manufacture so that the product
remains suitable for consumption within the period defined in a shelf‑life study.
PB  - Institute of Meat Hygiene and Technology
T2  - Meat Technology
T1  - Ensuring the safety of cooked and smoked sausages of a narrower diameter, in a cellulose casing, by heat treatment
VL  - 64
IS  - 2
SP  - 453
EP  - 459
DO  - 10.18485/meattech.2023.64.2.87
ER  - 

@article{
author = "Rašeta, Mladen and Branković-Lazić, Ivana and Mrdović, Boris and Simunović, Stefan and Simunović, Sara and Jovanović, Jelena and Becskei, Zsolt",
year = "2023",
abstract = "The aim of this work is to ensure the safety of finely chopped cooked sausages stuffed in a cellulose
casing, diameter 21 mm. Safety is ensured by thermal treatment of the level of pasteurization
in a chamber under a defined thermal treatment program. By monitoring the temperature
in the product’s thermal center at different control positions in the chamber, temperatures
of 75.26℃–77.11°C were determined with a thermocouple. In doing so, the products spent
8–9 minutes at a temperature higher than 70°C, and 18–20 minutes at a temperature higher
than 55°C. Considering that it is a small diameter product, the heat intensively penetrates to
the thermal center and thus ensures the safety of the product. However, due to the relatively
short time of the heat treatment, the pasteurization values (pv) at the control points were in the
range of 18.3–26 minutes. Therefore, we conclude that ensuring the safety of products with
a narrower diameter is achieved only by controlling the temperature in the thermal center of
the product, and not with pv values. Considering that this sausage is a perishable product,
it is necessary to provide a proper cold chain (0–4℃) after manufacture so that the product
remains suitable for consumption within the period defined in a shelf‑life study.",
publisher = "Institute of Meat Hygiene and Technology",
journal = "Meat Technology",
title = "Ensuring the safety of cooked and smoked sausages of a narrower diameter, in a cellulose casing, by heat treatment",
volume = "64",
number = "2",
pages = "453-459",
doi = "10.18485/meattech.2023.64.2.87"
}

Rašeta, M., Branković-Lazić, I., Mrdović, B., Simunović, S., Simunović, S., Jovanović, J.,& Becskei, Z.. (2023). Ensuring the safety of cooked and smoked sausages of a narrower diameter, in a cellulose casing, by heat treatment. in Meat Technology
Institute of Meat Hygiene and Technology., 64(2), 453-459.
https://doi.org/10.18485/meattech.2023.64.2.87

Rašeta M, Branković-Lazić I, Mrdović B, Simunović S, Simunović S, Jovanović J, Becskei Z. Ensuring the safety of cooked and smoked sausages of a narrower diameter, in a cellulose casing, by heat treatment. in Meat Technology. 2023;64(2):453-459.
doi:10.18485/meattech.2023.64.2.87 .

Rašeta, Mladen, Branković-Lazić, Ivana, Mrdović, Boris, Simunović, Stefan, Simunović, Sara, Jovanović, Jelena, Becskei, Zsolt, "Ensuring the safety of cooked and smoked sausages of a narrower diameter, in a cellulose casing, by heat treatment" in Meat Technology, 64, no. 2 (2023):453-459,
https://doi.org/10.18485/meattech.2023.64.2.87 . .