TY  - CONF
AU  - Glišić, Dimitrije
AU  - Milićević, Vesna
AU  - Krnjaić, Dejan
AU  - Prodanović, Radiša
AU  - Toplak, Ivan
AU  - Radojičić, Sonja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3208
AB  - Afrička kuga svinja (AKS) je virusno hemoragično oboljenje domaćih i divljih
svinja visokog stepena letaliteta, koje uzrokuje enormne ekonomske gubitke u
industriji svinja. Uzročnik afričke kuge je istoimeni virus, jedini pripadnik familije
Asfarviride i roda Asfivirus (Alonso i sar., 2018). Bolest je prvi put zabeležena u Srbiji
2019. godine u populaciji domaćih svinja, a od 2020. godine i u populaciji divljih svinja.
Od tada do danas broj svinja je smanjen za 7% što je više od prosečnog pada broja
svinja u Evropi od 4%. Genom virusa AKS je dvolančana linearna DNK dužine 170-190
kbp u zavisnosti od genotipa, podgenotipa i broja ponavljajućih segmenata i kodira
sintezu 170 virusnih proteina (Blome i sar., 2020). Održavanje virusa u prirodi zavisi
od faktora sredine, prisustva prijemčivih jedinki (divljih svinja) i otpornosti virusa.
Jedan od ključnih uslova za održavanje i cirkulaciju virusa je i visoka prevalencija
infekcije kod domaćih svinja (>5%) jer se na taj način omogućava prelivanje infekcije
na divlje svinje i zatvaranje kruga prenošenja (Aguilar-Vega i sar., 2023). Letalitet kod
divljih svinja inficiranih visoko virulentnim sojem iznosi 100%. Virus ostaje infektivan
3 meseca u leševima divljih svinja koje, ukoliko nisu uklonjene, dugotrajno
predstavljaju izvor infekcije. Brzina širenja infekcije u populaciji divljih svinja u Evropi
iznosi 4-17 km godišnje (Anette i sar., 2020). Širenje AKS na veće razdaljine uslovljeno
je antropogenim faktorom. Čovek kontaktom sa zaraženim ili uginulim divljim
svinjama, hranjenjem svinja kontaminiranim proizvodima animalnog porekla,
transportom zaraženih domaćih svinja iz jednog područija u drugo predstavlja
mehanički vektor za širenje virusa. Na afričkom kontinentu zabeležena su 24 genotipa
virusa koja cirkulišu između populacija bradavičastih svinja, mekih krpelja iz roda
Ornithodorus spp. i domaćih svinja. Van Afrike zabeleženi su samo genotip I i genotip
II virusa AKS. Za trenutnu epizootiju odgovoran je genotip II virusa. Autori Gallardo i
sar. (2023) su na osnovu analize genoma virusa AKS zabaležili 24 genogrupe u okviru genotipa II virusa na tlu Evrope. Na osnovu analize B646L i E183L gena opisan je jedan
genotip virusa (genotip II). Daljom analizom B602L gena definisana su dva
podgenotipa (CVR1 i 2) sa većim brojem varijacija koje zavise od broja tačkastih
mutacija. Na osnovu analize intergenskog segmenta (I73L - I329L), mogu se opisati
četiri podgenotipa (I-IV) (Gallardo i sar., 2023).
Laboratorijska dijagnostika afričke kuge se vrši primenom molekularnih metoda.
Cilj ovog rada je bila analiza cikulišućih sojeva virusa AKS u Srbiji. Trijažna testiranja
su vršena real-time PCR testom. Dok je dalja analiza vršena primenom klasičnog PCR
testa i Sanger sekvenciranja. Za detaljnu karakterizaciju lokalnih sojeva virusa AKS
odabrano je 95 uzoraka poreklom od domacih i divljih svinja obolelih od AKS u
periodu od 2019-2023 godine. Amplifikovana su i sekvencirana četiri segmenta
virusnog genoma (B646L, E183L, B602L i intergenski region između I73L - I329L). Na
osnovu analize ovih delova genoma utvrđeno je prisustvo više različitih sojeva virusa
u Srbiji. Istovremena cirkulacija nekoliko sojeva može biti posledica spontanih
mutacija ili reintrodukcije virusa iz drugih zaraženih područja. Utvrđivanje promena
u genskom materijalu smatraju se veoma značajnim informacijama jer mogu ukazivati
na adaptaciju virusa i modifikaciju virulentnosti. Praćenje promena u genomu virusa,
porsedno daje uvid u strukturu virusnih proteina. Dugotrajna cirkulacija virusa u
populaciji divljih svinja može dovesti do pojave srednje i niskovirulentnih sojeva što
predstavlja izazov za eradikaciju bolesti. Kod domaćih svinja, srednje i niskovirulentni
sojevi mogu izazvati atipične kliničke znakove i produženi tok bolesti što otežava
postavljanje sumnje, odlaže dijagnostiku i povećava rizik od širenja bolesti.
Potencijalne promene u konzerviranom delu genoma mogu otežati dijagnostiku
virusa. Stoga su izolacija i genska karakterizacija virusa afričke kuge svinja od
izuzetnog značaja za razumevanje epizootiologije ove bolesti. Poznavanje genskih
karakteristika lokalnih sojeva virusa omogućava praćenje njihovog evolutivnog
razvoja i identifikaciju novih sojeva. Na osnovu ovih podataka treba vršiti procenu
efikasnosti preduzetih mera za suzbijanje i kontrolu bolesti. Usled nedostatka
komercijalno dostupne vackcine, primena rigoroznih biosigurnostih mera je od
najvećeg značaja za sprečavanje unosa uzročnika u populaciju domaćih svinja. Stroge
kontrole na granicama su neophodne radi sprečavanja reintrodukcije virusa iz drugih
zaraženih područija, kao i brza reakcija u aktivnim žarištima radi sprečavanja daljeg
širenja bolesti i većih ekonomskih gubitaka.
AB  - African Swine Fever (ASF) is a highly lethal viral hemorrhagic disease affecting
domestic and wild pigs, causing significant economic losses in the swine industry. The
causative agent of ASF is a virus of the Asfarviridae family and Asfivirus genus (Alonso
et al., 2018). The disease was first recorded in Serbia in 2019 in domestic pig
populations, and since 2020, it has also been found in wild pig populations. Since then,
the number of pigs has decreased by 7%, which is higher than the average decline in
pig numbers in Europe (4%). The genome of the ASF virus is a double-stranded linear
DNA, ranging from 170-190 kbp in length, depending on the genotype, subgenotype,
and number of repetitive segments, encoding the synthesis of 170 viral proteins
(Blome et al., 2020). The maintenance of the virus in the environment depends on
environmental factors, the presence of susceptible individuals (wild pigs), and the
virus's ability to survive in that environment. A key condition for the maintenance and
circulation of the virus is a high prevalence of infection in domestic pigs (>5%), as it
enables the spill-over of infection to wild pigs, completing the transmission cycle
(Aguilar-Vega et al., 2023). The case fatality rate in wild pigs infected with highly
virulent strains is 100%. The virus remains infectious for 3 months in carcasses of wild
pigs, which, if not removed, serve as a long-term source of infection. The spread of
infection in the wild pig population in Europe is estimated at 4-17 km per year, with
anthropogenic factors playing a significant role in long-distance transmission (Anette
et al., 2020). Human contact with infected or dead wild pigs, feeding pigs with
contaminated animal-derived products, and the transport of infected domestic pigs
from one area to another act as mechanical vectors for virus spread. In Africa, 24
genotypes of the virus have been recorded, circulating among populations of bush
pigs, soft ticks of the Ornithodorus spp. genus, and domestic pigs. Outside of Africa,
only genotype I and genotype II of the ASF virus have been identified. The current epidemic is caused by the genotype II virus. Based on the genome analysis of the ASF
virus Gallardo et al. (2023), identified 24 genogroups within the genotype II virus in
Europe. From the analysis of the B646L and E183L genes, one virus strain (genotype
II) was described. Further analysis of the B602L gene defined two subgenotypes
(CVR1 and 2) with a higher number of variations depending on the number of point
mutations. Based on the analysis of the intergenic segment (I73L - I329L), four
subgenotypes (I-IV) can be described (Gallardo et al., 2023). Laboratory diagnostics of
African Swine Fever are conducted using molecular methods. The aim of this study
was to analyze circulating strains of ASF virus in Serbia. Screening tests were
performed using real-time PCR, while further analysis was conducted using
conventional PCR and Sanger sequencing. For a detailed characterization of local
strains of ASF virus, 95 samples from diseased domestic and wild pigs with ASF
between 2019 and 2023 were selected. Four segments of the viral genome (B646L,
E183L, B602L, and the intergenic region between I73L - I329L) were amplified and
sequenced. Based on the analysis of these genome segments, the presence of multiple
different strains of the virus was identified in Serbia. Simultaneous circulation of
several strains may result from spontaneous mutations or virus reintroduction from
other infected areas. Determining changes in the genetic material is considered highly
significant as they can indicate virus adaptation and modification of virulence.
Monitoring changes in the virus genome provides insight into the structure of viral
proteins. Prolonged circulation of the virus in the wild pig population can lead to the
emergence of moderately and low-virulent strains, posing challenges for disease
eradication. In domestic pigs, these strains can cause atypical clinical signs and
prolonged disease courses, complicating suspicion, delaying diagnosis, and increasing
the risk of disease spread. Potential changes in conserved regions of the genome can
hinder virus diagnostics. Therefore, the isolation and genetic characterization of ASF
virus strains are of utmost importance for understanding the epizootiology of this
disease. Knowledge of the genetic characteristics of local virus strains allows for
monitoring their evolutionary development and identification of new strains. Based
on these data, an assessment of the effectiveness of control measures should be
conducted. Due to the lack of commercially available vaccines, the implementation of
rigorous biosecurity measures is of paramount importance to prevent the
introduction of the pathogen into the domestic pig population. Strict controls at
borders are necessary to prevent the reintroduction of the virus from other infected
areas, along with swift responses in active outbreak areas to prevent further disease
spread and mitigate significant economic losses.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Genska varijabilnost virusa afričke kuge svinja u Srbiji
T1  - Genetic variability of African Swine Fever virus in Serbia
SP  - 24
EP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3208
ER  - 

@conference{
author = "Glišić, Dimitrije and Milićević, Vesna and Krnjaić, Dejan and Prodanović, Radiša and Toplak, Ivan and Radojičić, Sonja",
year = "2023",
abstract = "Afrička kuga svinja (AKS) je virusno hemoragično oboljenje domaćih i divljih
svinja visokog stepena letaliteta, koje uzrokuje enormne ekonomske gubitke u
industriji svinja. Uzročnik afričke kuge je istoimeni virus, jedini pripadnik familije
Asfarviride i roda Asfivirus (Alonso i sar., 2018). Bolest je prvi put zabeležena u Srbiji
2019. godine u populaciji domaćih svinja, a od 2020. godine i u populaciji divljih svinja.
Od tada do danas broj svinja je smanjen za 7% što je više od prosečnog pada broja
svinja u Evropi od 4%. Genom virusa AKS je dvolančana linearna DNK dužine 170-190
kbp u zavisnosti od genotipa, podgenotipa i broja ponavljajućih segmenata i kodira
sintezu 170 virusnih proteina (Blome i sar., 2020). Održavanje virusa u prirodi zavisi
od faktora sredine, prisustva prijemčivih jedinki (divljih svinja) i otpornosti virusa.
Jedan od ključnih uslova za održavanje i cirkulaciju virusa je i visoka prevalencija
infekcije kod domaćih svinja (>5%) jer se na taj način omogućava prelivanje infekcije
na divlje svinje i zatvaranje kruga prenošenja (Aguilar-Vega i sar., 2023). Letalitet kod
divljih svinja inficiranih visoko virulentnim sojem iznosi 100%. Virus ostaje infektivan
3 meseca u leševima divljih svinja koje, ukoliko nisu uklonjene, dugotrajno
predstavljaju izvor infekcije. Brzina širenja infekcije u populaciji divljih svinja u Evropi
iznosi 4-17 km godišnje (Anette i sar., 2020). Širenje AKS na veće razdaljine uslovljeno
je antropogenim faktorom. Čovek kontaktom sa zaraženim ili uginulim divljim
svinjama, hranjenjem svinja kontaminiranim proizvodima animalnog porekla,
transportom zaraženih domaćih svinja iz jednog područija u drugo predstavlja
mehanički vektor za širenje virusa. Na afričkom kontinentu zabeležena su 24 genotipa
virusa koja cirkulišu između populacija bradavičastih svinja, mekih krpelja iz roda
Ornithodorus spp. i domaćih svinja. Van Afrike zabeleženi su samo genotip I i genotip
II virusa AKS. Za trenutnu epizootiju odgovoran je genotip II virusa. Autori Gallardo i
sar. (2023) su na osnovu analize genoma virusa AKS zabaležili 24 genogrupe u okviru genotipa II virusa na tlu Evrope. Na osnovu analize B646L i E183L gena opisan je jedan
genotip virusa (genotip II). Daljom analizom B602L gena definisana su dva
podgenotipa (CVR1 i 2) sa većim brojem varijacija koje zavise od broja tačkastih
mutacija. Na osnovu analize intergenskog segmenta (I73L - I329L), mogu se opisati
četiri podgenotipa (I-IV) (Gallardo i sar., 2023).
Laboratorijska dijagnostika afričke kuge se vrši primenom molekularnih metoda.
Cilj ovog rada je bila analiza cikulišućih sojeva virusa AKS u Srbiji. Trijažna testiranja
su vršena real-time PCR testom. Dok je dalja analiza vršena primenom klasičnog PCR
testa i Sanger sekvenciranja. Za detaljnu karakterizaciju lokalnih sojeva virusa AKS
odabrano je 95 uzoraka poreklom od domacih i divljih svinja obolelih od AKS u
periodu od 2019-2023 godine. Amplifikovana su i sekvencirana četiri segmenta
virusnog genoma (B646L, E183L, B602L i intergenski region između I73L - I329L). Na
osnovu analize ovih delova genoma utvrđeno je prisustvo više različitih sojeva virusa
u Srbiji. Istovremena cirkulacija nekoliko sojeva može biti posledica spontanih
mutacija ili reintrodukcije virusa iz drugih zaraženih područja. Utvrđivanje promena
u genskom materijalu smatraju se veoma značajnim informacijama jer mogu ukazivati
na adaptaciju virusa i modifikaciju virulentnosti. Praćenje promena u genomu virusa,
porsedno daje uvid u strukturu virusnih proteina. Dugotrajna cirkulacija virusa u
populaciji divljih svinja može dovesti do pojave srednje i niskovirulentnih sojeva što
predstavlja izazov za eradikaciju bolesti. Kod domaćih svinja, srednje i niskovirulentni
sojevi mogu izazvati atipične kliničke znakove i produženi tok bolesti što otežava
postavljanje sumnje, odlaže dijagnostiku i povećava rizik od širenja bolesti.
Potencijalne promene u konzerviranom delu genoma mogu otežati dijagnostiku
virusa. Stoga su izolacija i genska karakterizacija virusa afričke kuge svinja od
izuzetnog značaja za razumevanje epizootiologije ove bolesti. Poznavanje genskih
karakteristika lokalnih sojeva virusa omogućava praćenje njihovog evolutivnog
razvoja i identifikaciju novih sojeva. Na osnovu ovih podataka treba vršiti procenu
efikasnosti preduzetih mera za suzbijanje i kontrolu bolesti. Usled nedostatka
komercijalno dostupne vackcine, primena rigoroznih biosigurnostih mera je od
najvećeg značaja za sprečavanje unosa uzročnika u populaciju domaćih svinja. Stroge
kontrole na granicama su neophodne radi sprečavanja reintrodukcije virusa iz drugih
zaraženih područija, kao i brza reakcija u aktivnim žarištima radi sprečavanja daljeg
širenja bolesti i većih ekonomskih gubitaka., African Swine Fever (ASF) is a highly lethal viral hemorrhagic disease affecting
domestic and wild pigs, causing significant economic losses in the swine industry. The
causative agent of ASF is a virus of the Asfarviridae family and Asfivirus genus (Alonso
et al., 2018). The disease was first recorded in Serbia in 2019 in domestic pig
populations, and since 2020, it has also been found in wild pig populations. Since then,
the number of pigs has decreased by 7%, which is higher than the average decline in
pig numbers in Europe (4%). The genome of the ASF virus is a double-stranded linear
DNA, ranging from 170-190 kbp in length, depending on the genotype, subgenotype,
and number of repetitive segments, encoding the synthesis of 170 viral proteins
(Blome et al., 2020). The maintenance of the virus in the environment depends on
environmental factors, the presence of susceptible individuals (wild pigs), and the
virus's ability to survive in that environment. A key condition for the maintenance and
circulation of the virus is a high prevalence of infection in domestic pigs (>5%), as it
enables the spill-over of infection to wild pigs, completing the transmission cycle
(Aguilar-Vega et al., 2023). The case fatality rate in wild pigs infected with highly
virulent strains is 100%. The virus remains infectious for 3 months in carcasses of wild
pigs, which, if not removed, serve as a long-term source of infection. The spread of
infection in the wild pig population in Europe is estimated at 4-17 km per year, with
anthropogenic factors playing a significant role in long-distance transmission (Anette
et al., 2020). Human contact with infected or dead wild pigs, feeding pigs with
contaminated animal-derived products, and the transport of infected domestic pigs
from one area to another act as mechanical vectors for virus spread. In Africa, 24
genotypes of the virus have been recorded, circulating among populations of bush
pigs, soft ticks of the Ornithodorus spp. genus, and domestic pigs. Outside of Africa,
only genotype I and genotype II of the ASF virus have been identified. The current epidemic is caused by the genotype II virus. Based on the genome analysis of the ASF
virus Gallardo et al. (2023), identified 24 genogroups within the genotype II virus in
Europe. From the analysis of the B646L and E183L genes, one virus strain (genotype
II) was described. Further analysis of the B602L gene defined two subgenotypes
(CVR1 and 2) with a higher number of variations depending on the number of point
mutations. Based on the analysis of the intergenic segment (I73L - I329L), four
subgenotypes (I-IV) can be described (Gallardo et al., 2023). Laboratory diagnostics of
African Swine Fever are conducted using molecular methods. The aim of this study
was to analyze circulating strains of ASF virus in Serbia. Screening tests were
performed using real-time PCR, while further analysis was conducted using
conventional PCR and Sanger sequencing. For a detailed characterization of local
strains of ASF virus, 95 samples from diseased domestic and wild pigs with ASF
between 2019 and 2023 were selected. Four segments of the viral genome (B646L,
E183L, B602L, and the intergenic region between I73L - I329L) were amplified and
sequenced. Based on the analysis of these genome segments, the presence of multiple
different strains of the virus was identified in Serbia. Simultaneous circulation of
several strains may result from spontaneous mutations or virus reintroduction from
other infected areas. Determining changes in the genetic material is considered highly
significant as they can indicate virus adaptation and modification of virulence.
Monitoring changes in the virus genome provides insight into the structure of viral
proteins. Prolonged circulation of the virus in the wild pig population can lead to the
emergence of moderately and low-virulent strains, posing challenges for disease
eradication. In domestic pigs, these strains can cause atypical clinical signs and
prolonged disease courses, complicating suspicion, delaying diagnosis, and increasing
the risk of disease spread. Potential changes in conserved regions of the genome can
hinder virus diagnostics. Therefore, the isolation and genetic characterization of ASF
virus strains are of utmost importance for understanding the epizootiology of this
disease. Knowledge of the genetic characteristics of local virus strains allows for
monitoring their evolutionary development and identification of new strains. Based
on these data, an assessment of the effectiveness of control measures should be
conducted. Due to the lack of commercially available vaccines, the implementation of
rigorous biosecurity measures is of paramount importance to prevent the
introduction of the pathogen into the domestic pig population. Strict controls at
borders are necessary to prevent the reintroduction of the virus from other infected
areas, along with swift responses in active outbreak areas to prevent further disease
spread and mitigate significant economic losses.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Genska varijabilnost virusa afričke kuge svinja u Srbiji, Genetic variability of African Swine Fever virus in Serbia",
pages = "24-28",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3208"
}

Glišić, D., Milićević, V., Krnjaić, D., Prodanović, R., Toplak, I.,& Radojičić, S.. (2023). Genska varijabilnost virusa afričke kuge svinja u Srbiji. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 24-28.
https://hdl.handle.net/21.15107/rcub_veterinar_3208

Glišić D, Milićević V, Krnjaić D, Prodanović R, Toplak I, Radojičić S. Genska varijabilnost virusa afričke kuge svinja u Srbiji. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:24-28.
https://hdl.handle.net/21.15107/rcub_veterinar_3208 .

Glišić, Dimitrije, Milićević, Vesna, Krnjaić, Dejan, Prodanović, Radiša, Toplak, Ivan, Radojičić, Sonja, "Genska varijabilnost virusa afričke kuge svinja u Srbiji" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):24-28,
https://hdl.handle.net/21.15107/rcub_veterinar_3208 .

TY  - JOUR
AU  - Ninković, Milan
AU  - Žutić, Jadranka
AU  - Bojkovski, Jovan
AU  - Arsić, Sveta
AU  - Glišić, Dimitrije
AU  - Zurovac Sapundžić, Zorana
AU  - Zdravković, Nemanja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3148
AB  - Th is case report describes the acute mastitis with signs of endotoxemia in the 32-month-old Simmental cow, antimicrobial susceptibility of
Klebsiella pneumoniae isolated from a milk sample, and demonstrates the
eff ectiveness of the applied therapy. Case presentation included the cowlying syndrome together with the presence of clinical signs of endotoxemia
accompanied by milk discoloration and “clots” formation. Bacteriology
fi nding of the K. pneumoniae and antibiogram pointed that the isolate was
sensitive to ceft riaxone, and trimethoprim/ sulfamethoxazole, while resistant to amoxicillin, gentamicin, ampicillin, ceft iofur, and cephalexin. Th e selection of treatment options came from the availability of an adequate route
of administration, urgency for treatment, and lack of time for assessment
of other body functions. Th e therapy by ceft riaxone at an intravenous dose
rate of 8mg/kg with adequate supportive therapy NSAID, vitamin C, and
correction dehydration showed a successful eff ect in life-saving procedures.
Th e outcome of K. pneumoniae mastitis with endotoxemia did not aff ect the
outcome of pregnancy.
PB  - Novi Sad : Scientific Institute for Veterinary Medicine
T2  - Archives of Veterinary Medicine
T1  - Acute bovine mastitis caused by Klebsiella pneumoniae – case report
T1  - Akutni goveđi mastitis izazvan bakterijom Klebsiella pneumoniae – prikaz slučaja
VL  - 16
IS  - 1
SP  - 97
EP  - 103
DO  - 10.46784/e-avm.v16i1.313
ER  - 

@article{
author = "Ninković, Milan and Žutić, Jadranka and Bojkovski, Jovan and Arsić, Sveta and Glišić, Dimitrije and Zurovac Sapundžić, Zorana and Zdravković, Nemanja",
year = "2023",
abstract = "Th is case report describes the acute mastitis with signs of endotoxemia in the 32-month-old Simmental cow, antimicrobial susceptibility of
Klebsiella pneumoniae isolated from a milk sample, and demonstrates the
eff ectiveness of the applied therapy. Case presentation included the cowlying syndrome together with the presence of clinical signs of endotoxemia
accompanied by milk discoloration and “clots” formation. Bacteriology
fi nding of the K. pneumoniae and antibiogram pointed that the isolate was
sensitive to ceft riaxone, and trimethoprim/ sulfamethoxazole, while resistant to amoxicillin, gentamicin, ampicillin, ceft iofur, and cephalexin. Th e selection of treatment options came from the availability of an adequate route
of administration, urgency for treatment, and lack of time for assessment
of other body functions. Th e therapy by ceft riaxone at an intravenous dose
rate of 8mg/kg with adequate supportive therapy NSAID, vitamin C, and
correction dehydration showed a successful eff ect in life-saving procedures.
Th e outcome of K. pneumoniae mastitis with endotoxemia did not aff ect the
outcome of pregnancy.",
publisher = "Novi Sad : Scientific Institute for Veterinary Medicine",
journal = "Archives of Veterinary Medicine",
title = "Acute bovine mastitis caused by Klebsiella pneumoniae – case report, Akutni goveđi mastitis izazvan bakterijom Klebsiella pneumoniae – prikaz slučaja",
volume = "16",
number = "1",
pages = "97-103",
doi = "10.46784/e-avm.v16i1.313"
}

Ninković, M., Žutić, J., Bojkovski, J., Arsić, S., Glišić, D., Zurovac Sapundžić, Z.,& Zdravković, N.. (2023). Acute bovine mastitis caused by Klebsiella pneumoniae – case report. in Archives of Veterinary Medicine
Novi Sad : Scientific Institute for Veterinary Medicine., 16(1), 97-103.
https://doi.org/10.46784/e-avm.v16i1.313

Ninković M, Žutić J, Bojkovski J, Arsić S, Glišić D, Zurovac Sapundžić Z, Zdravković N. Acute bovine mastitis caused by Klebsiella pneumoniae – case report. in Archives of Veterinary Medicine. 2023;16(1):97-103.
doi:10.46784/e-avm.v16i1.313 .

Ninković, Milan, Žutić, Jadranka, Bojkovski, Jovan, Arsić, Sveta, Glišić, Dimitrije, Zurovac Sapundžić, Zorana, Zdravković, Nemanja, "Acute bovine mastitis caused by Klebsiella pneumoniae – case report" in Archives of Veterinary Medicine, 16, no. 1 (2023):97-103,
https://doi.org/10.46784/e-avm.v16i1.313 . .

TY  - JOUR
AU  - Glišić, Dimitrije
AU  - Milićević, Vesna
AU  - Veljović, Ljubiša
AU  - Milovanović, Bojan
AU  - Kureljušić, Branislav
AU  - Ðordević, Igor
AU  - Anđelković, Katarina
AU  - Petković, Jelena
AU  - Dačić, Miroljub
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2540
AB  - The first case of African swine fever in domestic pigs in Serbia was in 2019. The following
year, the disease was confirmed in wild boar. Thenceforth, ASF has been continuously reported in
both wild and domestic pigs. The outbreaks in domestic pigs could not be linked directly to wild
boars, even though wild boars were endemically infected, and reservoirs for ASF. This study aimed to
investigate outbreaks and routes of transmission in domestic pigs in a region of central Serbia where
no outbreaks in wild boar were reported. Fourteen outbreaks of ASF on backyard farms with low
biosecurity were traced back, and no connection to wild boar was found. The epidemic investigation
covered 2094 holdings, with 24,368 pigs, out of which 1882 were tested for ASF. In surrounding
hunting grounds, field searches were conducted. Dead wild boars were found, and 138 hunted wild
boars were negative for ASFV. It was concluded that outbreaks in 2021 were provoked by the illegal
trade of live animals and pig products. Even though infective pressure from wild boars is assumed,
no positive cases have been found, while the ASFV spreads within the domestic swine population
evidenced in four recent outbreaks in 2022
PB  - MDPI
T2  - Pathogens
T1  - Patterns of ASFV Transmission in Domestic Pigs in Serbia
VL  - 12
IS  - 149
DO  - 10.3390/pathogens12010149
ER  - 

@article{
author = "Glišić, Dimitrije and Milićević, Vesna and Veljović, Ljubiša and Milovanović, Bojan and Kureljušić, Branislav and Ðordević, Igor and Anđelković, Katarina and Petković, Jelena and Dačić, Miroljub",
year = "2023",
abstract = "The first case of African swine fever in domestic pigs in Serbia was in 2019. The following
year, the disease was confirmed in wild boar. Thenceforth, ASF has been continuously reported in
both wild and domestic pigs. The outbreaks in domestic pigs could not be linked directly to wild
boars, even though wild boars were endemically infected, and reservoirs for ASF. This study aimed to
investigate outbreaks and routes of transmission in domestic pigs in a region of central Serbia where
no outbreaks in wild boar were reported. Fourteen outbreaks of ASF on backyard farms with low
biosecurity were traced back, and no connection to wild boar was found. The epidemic investigation
covered 2094 holdings, with 24,368 pigs, out of which 1882 were tested for ASF. In surrounding
hunting grounds, field searches were conducted. Dead wild boars were found, and 138 hunted wild
boars were negative for ASFV. It was concluded that outbreaks in 2021 were provoked by the illegal
trade of live animals and pig products. Even though infective pressure from wild boars is assumed,
no positive cases have been found, while the ASFV spreads within the domestic swine population
evidenced in four recent outbreaks in 2022",
publisher = "MDPI",
journal = "Pathogens",
title = "Patterns of ASFV Transmission in Domestic Pigs in Serbia",
volume = "12",
number = "149",
doi = "10.3390/pathogens12010149"
}

Glišić, D., Milićević, V., Veljović, L., Milovanović, B., Kureljušić, B., Ðordević, I., Anđelković, K., Petković, J.,& Dačić, M.. (2023). Patterns of ASFV Transmission in Domestic Pigs in Serbia. in Pathogens
MDPI., 12(149).
https://doi.org/10.3390/pathogens12010149

Glišić D, Milićević V, Veljović L, Milovanović B, Kureljušić B, Ðordević I, Anđelković K, Petković J, Dačić M. Patterns of ASFV Transmission in Domestic Pigs in Serbia. in Pathogens. 2023;12(149).
doi:10.3390/pathogens12010149 .

Glišić, Dimitrije, Milićević, Vesna, Veljović, Ljubiša, Milovanović, Bojan, Kureljušić, Branislav, Ðordević, Igor, Anđelković, Katarina, Petković, Jelena, Dačić, Miroljub, "Patterns of ASFV Transmission in Domestic Pigs in Serbia" in Pathogens, 12, no. 149 (2023),
https://doi.org/10.3390/pathogens12010149 . .

TY  - JOUR
AU  - Milovanović, Bojan
AU  - Đorđević, Milutin
AU  - Savić, Božidar
AU  - Jezdimirović, Nemanja
AU  - Zdravković, Nemanja
AU  - Sapundžić Zurovac, Zorana
AU  - Glišić, Dimitrije
AU  - Ninković, Milan
AU  - Kureljušić, Branislav
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2830
AB  - A female mouflon, weighing 40 kilograms, was submitted to the diagnostic laboratory of the Institute of Veterinary Medicine of Serbia for determining the potential cause of death. Necropsy revealed massive hemorrhages in subcutaneous andintermuscular tissue and on papillary muscle. Petechiae and ecchymoses were found on the omentum, mesentery and adipose tissue of heart, kidneyand costal pleura.Haemorrhagic-necrotic enteritis in duodenum and jejunum was characterized by catarrhal hemorrhagic inflammation withthe presence of mucous and bloody content, whereas gas bubbles in the submucosa have also been confirmed. Bacterial cultures from sampled organs were identified as Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelli.Based on the established pathological and histological changes and the results of the bacteriological, biochemical, and molecular examination, the state of septic shock and toxemia with disseminated massive bleeding was the immediate cause of mouflon death. The septic condition is a consequence of enterotoxemia caused by Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelliinfection.
PB  - Hellenic Veterinary Medical Society
T2  - Journal of the Hellenic Veterinary Medical Society
T1  - Concurrent infection by Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelli in mouflon (Ovis aries musimon) - a case report
VL  - 73
IS  - 4
SP  - 5067
EP  - 5072
DO  - 10.12681/jhvms.28086
ER  - 

@article{
author = "Milovanović, Bojan and Đorđević, Milutin and Savić, Božidar and Jezdimirović, Nemanja and Zdravković, Nemanja and Sapundžić Zurovac, Zorana and Glišić, Dimitrije and Ninković, Milan and Kureljušić, Branislav",
year = "2022",
abstract = "A female mouflon, weighing 40 kilograms, was submitted to the diagnostic laboratory of the Institute of Veterinary Medicine of Serbia for determining the potential cause of death. Necropsy revealed massive hemorrhages in subcutaneous andintermuscular tissue and on papillary muscle. Petechiae and ecchymoses were found on the omentum, mesentery and adipose tissue of heart, kidneyand costal pleura.Haemorrhagic-necrotic enteritis in duodenum and jejunum was characterized by catarrhal hemorrhagic inflammation withthe presence of mucous and bloody content, whereas gas bubbles in the submucosa have also been confirmed. Bacterial cultures from sampled organs were identified as Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelli.Based on the established pathological and histological changes and the results of the bacteriological, biochemical, and molecular examination, the state of septic shock and toxemia with disseminated massive bleeding was the immediate cause of mouflon death. The septic condition is a consequence of enterotoxemia caused by Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelliinfection.",
publisher = "Hellenic Veterinary Medical Society",
journal = "Journal of the Hellenic Veterinary Medical Society",
title = "Concurrent infection by Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelli in mouflon (Ovis aries musimon) - a case report",
volume = "73",
number = "4",
pages = "5067-5072",
doi = "10.12681/jhvms.28086"
}

Milovanović, B., Đorđević, M., Savić, B., Jezdimirović, N., Zdravković, N., Sapundžić Zurovac, Z., Glišić, D., Ninković, M.,& Kureljušić, B.. (2022). Concurrent infection by Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelli in mouflon (Ovis aries musimon) - a case report. in Journal of the Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Society., 73(4), 5067-5072.
https://doi.org/10.12681/jhvms.28086

Milovanović B, Đorđević M, Savić B, Jezdimirović N, Zdravković N, Sapundžić Zurovac Z, Glišić D, Ninković M, Kureljušić B. Concurrent infection by Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelli in mouflon (Ovis aries musimon) - a case report. in Journal of the Hellenic Veterinary Medical Society. 2022;73(4):5067-5072.
doi:10.12681/jhvms.28086 .

Milovanović, Bojan, Đorđević, Milutin, Savić, Božidar, Jezdimirović, Nemanja, Zdravković, Nemanja, Sapundžić Zurovac, Zorana, Glišić, Dimitrije, Ninković, Milan, Kureljušić, Branislav, "Concurrent infection by Clostridium perfringens type A, Clostridium septicum, and Clostridium sordelli in mouflon (Ovis aries musimon) - a case report" in Journal of the Hellenic Veterinary Medical Society, 73, no. 4 (2022):5067-5072,
https://doi.org/10.12681/jhvms.28086 . .

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Milićević, Vesna
AU  - Ćirović, Duško
AU  - Kuručki, Milica
AU  - Glišić, Dimitrije
AU  - Sapundžić Zurovac, Zorana
AU  - Milovanović, Bojan
AU  - Weissenbacher-Lang, Christiane
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2545
AB  - Forty-six golden jackals (Canis aureus) were collected between November 2020 and February 2021 in five counties of Serbia. Lung samples were screened for the presence of Pneumocystis DNA by pan-Pneumocystis PCR on the mtLSU rRNA gene obtaining PCR products of 370 bp in length. Pneumocystis DNA was detected in the lungs from 6/46 (13.04%) golden jackals. Four were females and two were males; four were classified as adults and two as subadults. Positive samples were confirmed in 4/5 investigated counties. No gross pathologic lung lesions were observed in this study. The sequences of Pneumocystis spp. from golden jackals were identical to one another and showed the highest similarity with Pneumocystis spp. sequences of dogs (98% nucleotide identity). The genetic variation was comparable to Pneumocystis spp. of raccoon dogs (95-97% nucleotide identity), red foxes (91-95% nucleotide identity), ferrets (86% nucleotide identity), and another Pneumocystis type in dogs (P. canis Ck2, 81% nucleotide identity) was higher. Golden jackals may be carriers and may play a nonnegligible role in the spread of Pneumocystis spp. Although this finding cannot be directly related to any clinical manifestation or pathologic lesions, a possible role in the exacerbation of different pulmonary disorders should be considered.
PB  - Wildlife Disease Association
T2  - Journal of wildlife diseases
T1  - First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus)
VL  - 58
IS  - 4
SP  - 897
EP  - 901
DO  - 10.7589/JWD-D-22-00007
ER  - 

@article{
author = "Kureljušić, Branislav and Milićević, Vesna and Ćirović, Duško and Kuručki, Milica and Glišić, Dimitrije and Sapundžić Zurovac, Zorana and Milovanović, Bojan and Weissenbacher-Lang, Christiane",
year = "2022",
abstract = "Forty-six golden jackals (Canis aureus) were collected between November 2020 and February 2021 in five counties of Serbia. Lung samples were screened for the presence of Pneumocystis DNA by pan-Pneumocystis PCR on the mtLSU rRNA gene obtaining PCR products of 370 bp in length. Pneumocystis DNA was detected in the lungs from 6/46 (13.04%) golden jackals. Four were females and two were males; four were classified as adults and two as subadults. Positive samples were confirmed in 4/5 investigated counties. No gross pathologic lung lesions were observed in this study. The sequences of Pneumocystis spp. from golden jackals were identical to one another and showed the highest similarity with Pneumocystis spp. sequences of dogs (98% nucleotide identity). The genetic variation was comparable to Pneumocystis spp. of raccoon dogs (95-97% nucleotide identity), red foxes (91-95% nucleotide identity), ferrets (86% nucleotide identity), and another Pneumocystis type in dogs (P. canis Ck2, 81% nucleotide identity) was higher. Golden jackals may be carriers and may play a nonnegligible role in the spread of Pneumocystis spp. Although this finding cannot be directly related to any clinical manifestation or pathologic lesions, a possible role in the exacerbation of different pulmonary disorders should be considered.",
publisher = "Wildlife Disease Association",
journal = "Journal of wildlife diseases",
title = "First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus)",
volume = "58",
number = "4",
pages = "897-901",
doi = "10.7589/JWD-D-22-00007"
}

Kureljušić, B., Milićević, V., Ćirović, D., Kuručki, M., Glišić, D., Sapundžić Zurovac, Z., Milovanović, B.,& Weissenbacher-Lang, C.. (2022). First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus). in Journal of wildlife diseases
Wildlife Disease Association., 58(4), 897-901.
https://doi.org/10.7589/JWD-D-22-00007

Kureljušić B, Milićević V, Ćirović D, Kuručki M, Glišić D, Sapundžić Zurovac Z, Milovanović B, Weissenbacher-Lang C. First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus). in Journal of wildlife diseases. 2022;58(4):897-901.
doi:10.7589/JWD-D-22-00007 .

Kureljušić, Branislav, Milićević, Vesna, Ćirović, Duško, Kuručki, Milica, Glišić, Dimitrije, Sapundžić Zurovac, Zorana, Milovanović, Bojan, Weissenbacher-Lang, Christiane, "First Molecular Detection of Pneumocystis spp. in the Golden Jackal (Canis aureus)" in Journal of wildlife diseases, 58, no. 4 (2022):897-901,
https://doi.org/10.7589/JWD-D-22-00007 . .

TY  - JOUR
AU  - Ninković, Milan
AU  - Arsić, Sveta
AU  - Žutić, Jadranka
AU  - Zdravković, Nemanja
AU  - Glišić, Dimitrije
AU  - Sapundžić Zurovac, Zorana
AU  - Bojkovski, Jovan
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2578
AB  - Lameness as a consequence of hoof disorders is gaining significance in animal health considerations with a particular impact in tie-hold systems. Preventive hoof trimming becomes an important zootechnic measure and procedure for the health and welfare improvement of cows. The objective of our study was to demonstrate findings of sole ulcers and white line diseases of cattle on smallholder farms. Another aspect was to display our observations on hoof trimming within the perspective of sole ulcers and white line disease in the examined herds. A total of 108 Simmental cows from 14 small noncommercial farms located in the Mačva district, Western Serbia, from March to October in 2020 were observed. The trimming procedure was performed by the Dutch method with cows fixed in a mobile chute for hoof trimming. Also, medical treatment of the hoof was provided as needed, using orthopedic foot blocks (wooden and rubberized blocks). Before treatment the white line disease and sole ulcers were present in 12 herds (85.7%). After treatment, there was a decrease in the frequency of sole ulcers positive herds (from 85.7% to 78.5%), while the frequency of white line disease in herds remained unchanged. Regarding cows, a frequency of white line disease of 25.0% was reduced to 18.5%, showing a significant difference (p<0.05) before and after treatment. The frequency of sole ulcers in cows of 23.1% was reduced to 15.7% on re-examination six months after trimming though the difference was not significant, respectively (P<0.05). White line disease and sole ulcers are widespread diseases of hooves on small noncommercial farms in Serbia. This study also provides insights that show the health status of cows’ hooves and demonstrated the importance of regular hoof trimming. Our findings suggest that regular hoof trimming (twice a year) and adequate housing conditions are crucial for improving the health of hooves.
PB  - Massimo Morgante
T2  - Large Animal Review
T1  - Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows
VL  - 27
IS  - 6
SP  - 329
EP  - 332
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2578
ER  - 

@article{
author = "Ninković, Milan and Arsić, Sveta and Žutić, Jadranka and Zdravković, Nemanja and Glišić, Dimitrije and Sapundžić Zurovac, Zorana and Bojkovski, Jovan",
year = "2021",
abstract = "Lameness as a consequence of hoof disorders is gaining significance in animal health considerations with a particular impact in tie-hold systems. Preventive hoof trimming becomes an important zootechnic measure and procedure for the health and welfare improvement of cows. The objective of our study was to demonstrate findings of sole ulcers and white line diseases of cattle on smallholder farms. Another aspect was to display our observations on hoof trimming within the perspective of sole ulcers and white line disease in the examined herds. A total of 108 Simmental cows from 14 small noncommercial farms located in the Mačva district, Western Serbia, from March to October in 2020 were observed. The trimming procedure was performed by the Dutch method with cows fixed in a mobile chute for hoof trimming. Also, medical treatment of the hoof was provided as needed, using orthopedic foot blocks (wooden and rubberized blocks). Before treatment the white line disease and sole ulcers were present in 12 herds (85.7%). After treatment, there was a decrease in the frequency of sole ulcers positive herds (from 85.7% to 78.5%), while the frequency of white line disease in herds remained unchanged. Regarding cows, a frequency of white line disease of 25.0% was reduced to 18.5%, showing a significant difference (p<0.05) before and after treatment. The frequency of sole ulcers in cows of 23.1% was reduced to 15.7% on re-examination six months after trimming though the difference was not significant, respectively (P<0.05). White line disease and sole ulcers are widespread diseases of hooves on small noncommercial farms in Serbia. This study also provides insights that show the health status of cows’ hooves and demonstrated the importance of regular hoof trimming. Our findings suggest that regular hoof trimming (twice a year) and adequate housing conditions are crucial for improving the health of hooves.",
publisher = "Massimo Morgante",
journal = "Large Animal Review",
title = "Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows",
volume = "27",
number = "6",
pages = "329-332",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2578"
}

Ninković, M., Arsić, S., Žutić, J., Zdravković, N., Glišić, D., Sapundžić Zurovac, Z.,& Bojkovski, J.. (2021). Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows. in Large Animal Review
Massimo Morgante., 27(6), 329-332.
https://hdl.handle.net/21.15107/rcub_veterinar_2578

Ninković M, Arsić S, Žutić J, Zdravković N, Glišić D, Sapundžić Zurovac Z, Bojkovski J. Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows. in Large Animal Review. 2021;27(6):329-332.
https://hdl.handle.net/21.15107/rcub_veterinar_2578 .

Ninković, Milan, Arsić, Sveta, Žutić, Jadranka, Zdravković, Nemanja, Glišić, Dimitrije, Sapundžić Zurovac, Zorana, Bojkovski, Jovan, "Frequency of White line disease and Sole ulcers and impact of hoof trimming in examined herds of Simmental cows" in Large Animal Review, 27, no. 6 (2021):329-332,
https://hdl.handle.net/21.15107/rcub_veterinar_2578 .