TY  - JOUR
AU  - Epps, Sharon V. R.
AU  - Harvey, Roger B.
AU  - Byrd, J. Allen
AU  - Petrujkić, Branko
AU  - Sedej, Ivana
AU  - Beier, Ross C.
AU  - Phillips, Timothy D.
AU  - Hume, Michael E.
AU  - Anderson, Robin C.
AU  - Nisbet, David J.
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1226
AB  - Campylobacter jejuni is an important human food-borne pathogen that can contaminate meat and poultry during processing. Consequently, strategies are sought to reduce the carriage of C. jejuni in food animals before they arrive at the abattoir. Thymol is a natural product that reduces survivability of Campylobacter in vitro, but its rapid absorption from the proximal alimentary tract limits its bactericidal efficacy in vivo. Thymol-beta-d-glucopyranoside is more resistant to absorption than free thymol, but its administration to chickens has not been reported. In the present studies, 1mM thymol-beta-d-glucopyranoside was shown to exhibit near equal anti-Campylobacter activity as 1mM thymol when incubated anaerobically in avian crop or cecal contents in vitro, resulting in reductions of 1.10-2.32 log(10) colony forming units mL(-1) in C. jejuni concentrations after 24h incubation. In a follow-up live animal study, oral administration of thymol-beta-d-glucopyranoside, but not free thymol, significantly lowered (>10-fold) recovery of Campylobacter from the crop of market-aged broilers when compared to placebo-treated controls (n = 6 broilers/treatment). Neither thymol-beta-d-glucopyranoside nor thymol affected recovery of Campylobacter from cecal contents of the treated broilers. These results indicate that rapid absorption or passage of free thymol from the crop precluded its anti-Campylobacter activity at this site and throughout the entire gastrointestinal tract. Conversely, lower recovery of Campylobacter from the crop of birds treated with thymol-beta-d-glucopyranoside indicates this conjugate was retained and able to be hydrolyzed to biologically active free thymol at this site as intended, yet was not sufficiently protected to allow passage of efficacious amounts of the intact glycoside to the lower gut. Nevertheless, these results warrant further research to see if higher doses or encapsulation of thymol-beta-d-glucopyranoside or similar glycosides may yield an efficacious additive to reduce carriage of Campylobacter as well as other pathogens throughout the avian gut.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural
T1  - Comparative effect of thymol or its glucose conjugate, thymol-beta-d-glucopyranoside, on Campylobacter in avian gut contents
VL  - 50
IS  - 1
SP  - 55
EP  - 61
DO  - 10.1080/03601234.2015.965634
ER  - 

@article{
author = "Epps, Sharon V. R. and Harvey, Roger B. and Byrd, J. Allen and Petrujkić, Branko and Sedej, Ivana and Beier, Ross C. and Phillips, Timothy D. and Hume, Michael E. and Anderson, Robin C. and Nisbet, David J.",
year = "2015",
abstract = "Campylobacter jejuni is an important human food-borne pathogen that can contaminate meat and poultry during processing. Consequently, strategies are sought to reduce the carriage of C. jejuni in food animals before they arrive at the abattoir. Thymol is a natural product that reduces survivability of Campylobacter in vitro, but its rapid absorption from the proximal alimentary tract limits its bactericidal efficacy in vivo. Thymol-beta-d-glucopyranoside is more resistant to absorption than free thymol, but its administration to chickens has not been reported. In the present studies, 1mM thymol-beta-d-glucopyranoside was shown to exhibit near equal anti-Campylobacter activity as 1mM thymol when incubated anaerobically in avian crop or cecal contents in vitro, resulting in reductions of 1.10-2.32 log(10) colony forming units mL(-1) in C. jejuni concentrations after 24h incubation. In a follow-up live animal study, oral administration of thymol-beta-d-glucopyranoside, but not free thymol, significantly lowered (>10-fold) recovery of Campylobacter from the crop of market-aged broilers when compared to placebo-treated controls (n = 6 broilers/treatment). Neither thymol-beta-d-glucopyranoside nor thymol affected recovery of Campylobacter from cecal contents of the treated broilers. These results indicate that rapid absorption or passage of free thymol from the crop precluded its anti-Campylobacter activity at this site and throughout the entire gastrointestinal tract. Conversely, lower recovery of Campylobacter from the crop of birds treated with thymol-beta-d-glucopyranoside indicates this conjugate was retained and able to be hydrolyzed to biologically active free thymol at this site as intended, yet was not sufficiently protected to allow passage of efficacious amounts of the intact glycoside to the lower gut. Nevertheless, these results warrant further research to see if higher doses or encapsulation of thymol-beta-d-glucopyranoside or similar glycosides may yield an efficacious additive to reduce carriage of Campylobacter as well as other pathogens throughout the avian gut.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural",
title = "Comparative effect of thymol or its glucose conjugate, thymol-beta-d-glucopyranoside, on Campylobacter in avian gut contents",
volume = "50",
number = "1",
pages = "55-61",
doi = "10.1080/03601234.2015.965634"
}

Epps, S. V. R., Harvey, R. B., Byrd, J. A., Petrujkić, B., Sedej, I., Beier, R. C., Phillips, T. D., Hume, M. E., Anderson, R. C.,& Nisbet, D. J.. (2015). Comparative effect of thymol or its glucose conjugate, thymol-beta-d-glucopyranoside, on Campylobacter in avian gut contents. in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural
Taylor & Francis Inc, Philadelphia., 50(1), 55-61.
https://doi.org/10.1080/03601234.2015.965634

Epps SVR, Harvey RB, Byrd JA, Petrujkić B, Sedej I, Beier RC, Phillips TD, Hume ME, Anderson RC, Nisbet DJ. Comparative effect of thymol or its glucose conjugate, thymol-beta-d-glucopyranoside, on Campylobacter in avian gut contents. in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural. 2015;50(1):55-61.
doi:10.1080/03601234.2015.965634 .

Epps, Sharon V. R., Harvey, Roger B., Byrd, J. Allen, Petrujkić, Branko, Sedej, Ivana, Beier, Ross C., Phillips, Timothy D., Hume, Michael E., Anderson, Robin C., Nisbet, David J., "Comparative effect of thymol or its glucose conjugate, thymol-beta-d-glucopyranoside, on Campylobacter in avian gut contents" in Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural, 50, no. 1 (2015):55-61,
https://doi.org/10.1080/03601234.2015.965634 . .