TY  - CONF
AU  - Aleksić-Kovačević, Sanja
AU  - Gligorić, Srđan
AU  - Kukolj, Vladimir
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3060
AB  - Ретроспективна и опсервациона анализа података о туморима паса спроведена
је на узорцима тумора достављеним Лабораторији за патологију, Факултета
ветеринарске медицине, Универзитета у Београду од 2017. до 2022. године.
Дескриптивна епидемиолошка анализа обухватала је податке који се односе на:
расу, узраст, пол и особине тумора. Мада се учесталост неоплазми повећава са
старењем, веома мало информација је доступно о појави неоплазми код паса до
12 месеци старости, као и код младих паса у узрасту до 24 месеца.
Наша испитивања указују на присуство различитих типова бенигних и
малигних тумора код младих паса до 12 месеци старости. Представљен је
ретроспективни преглед хистопатолошких дијагноза тумора достављених
нашој лабораторији током последњих пет година код две групе младих паса: у
старосној доби до 12 месеци и код паса од 13 до 24 месеца старости.
Од укупно 2435 неоплазми паса различитог ткивног порекла, 2,26% је
идентификовано код паса до 12 месеци старости и 4,02% код паса старости од
13 до 24 месеца. У старосној групи до 12 месеци, 80% тумора су били бенигни,
а 20% малигни, а код оних узраста 13 до 24 месеца, учесталост бенигних
неоплазми била је 67,35%, док су малигни чинили већ 32,65% свих тумора.
Хистиоцитом коже паса је био најчешће присутан тумор у обе групе младих
паса. Фибросарком је дијагностикован код женке вајмарског птичара, старе
само четири месеца. Неопластични процеси код младих паса најчешће су били
заступљени код расе француски булдог и код мешанаца. Наведена истраживања
из области ветеринарске онколошке патологије, дају драгоцене епидемиолошке
податке о туморима који се јављају код младих паса у Републици Србији.
AB  - According to WHO reports, tumors most often occur in older dogs between 9 and 12
years of age, with different breed predispositions for certain types of neoplasms. A
retrospective and cross-sectional study of canine tumors was performed at Pathology
Department, Faculty of Veterinary Medicine, Belgrade University, from 2017-2022.
Descriptive epidemiology was made from all data: breed, age, sex, and tumor features.
Although the incidence of neoplasms increases with age, little information is available
on the occurrence of neoplasms in dogs up to the age of 12 as well as 24 months.
Current study specimens revealed presence of different types of benign and malignant
tumors in dogs up to 12 months. Retrospective review of histopathological diagnoses
of neoplastic tissue samples submitted in last five years at Laboratory for pathology,
Faculty of veterinary Medicine, University of Belgrade, is performed having special
attention on: group up to the age of 12 months and group from 13 to 24 months.
In total of 2435 neoplasms found in different dog tissues, 2.26% were identified in
dogs up to age of 12 months and 4.02% in dogs aged from 13 to 24 months. In the
present analysis in the group up to 12 months 80% of tumors were benign, and 20%
were malignant followed by 67.35% benign neoplasms and 32,65% malignant in dogs
aged from 13 to 24 months. Canine cutaneous histiocytoma was the most frequent in
both of groups. Fibrosarcoma have been reported in only four months old female
Weimaraner. French bulldog and mixed-breed dogs were the most frequently affected.
These obtained data in field of veterinary oncology provide valuable epidemiological
information on neoplasms occurring in young dogs in Republic of Serbia.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
T1  - Epidemiološka ispitivanja tumora mladih pasa u periodu od 2017. do 2022. godine u Republici Srbiji
T1  - Epidemiology of tumours in young dogs in Republic of Serbia from 2017-2022
SP  - 128
EP  - 129
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3060
ER  - 

@conference{
author = "Aleksić-Kovačević, Sanja and Gligorić, Srđan and Kukolj, Vladimir",
year = "2023",
abstract = "Ретроспективна и опсервациона анализа података о туморима паса спроведена
је на узорцима тумора достављеним Лабораторији за патологију, Факултета
ветеринарске медицине, Универзитета у Београду од 2017. до 2022. године.
Дескриптивна епидемиолошка анализа обухватала је податке који се односе на:
расу, узраст, пол и особине тумора. Мада се учесталост неоплазми повећава са
старењем, веома мало информација је доступно о појави неоплазми код паса до
12 месеци старости, као и код младих паса у узрасту до 24 месеца.
Наша испитивања указују на присуство различитих типова бенигних и
малигних тумора код младих паса до 12 месеци старости. Представљен је
ретроспективни преглед хистопатолошких дијагноза тумора достављених
нашој лабораторији током последњих пет година код две групе младих паса: у
старосној доби до 12 месеци и код паса од 13 до 24 месеца старости.
Од укупно 2435 неоплазми паса различитог ткивног порекла, 2,26% је
идентификовано код паса до 12 месеци старости и 4,02% код паса старости од
13 до 24 месеца. У старосној групи до 12 месеци, 80% тумора су били бенигни,
а 20% малигни, а код оних узраста 13 до 24 месеца, учесталост бенигних
неоплазми била је 67,35%, док су малигни чинили већ 32,65% свих тумора.
Хистиоцитом коже паса је био најчешће присутан тумор у обе групе младих
паса. Фибросарком је дијагностикован код женке вајмарског птичара, старе
само четири месеца. Неопластични процеси код младих паса најчешће су били
заступљени код расе француски булдог и код мешанаца. Наведена истраживања
из области ветеринарске онколошке патологије, дају драгоцене епидемиолошке
податке о туморима који се јављају код младих паса у Републици Србији., According to WHO reports, tumors most often occur in older dogs between 9 and 12
years of age, with different breed predispositions for certain types of neoplasms. A
retrospective and cross-sectional study of canine tumors was performed at Pathology
Department, Faculty of Veterinary Medicine, Belgrade University, from 2017-2022.
Descriptive epidemiology was made from all data: breed, age, sex, and tumor features.
Although the incidence of neoplasms increases with age, little information is available
on the occurrence of neoplasms in dogs up to the age of 12 as well as 24 months.
Current study specimens revealed presence of different types of benign and malignant
tumors in dogs up to 12 months. Retrospective review of histopathological diagnoses
of neoplastic tissue samples submitted in last five years at Laboratory for pathology,
Faculty of veterinary Medicine, University of Belgrade, is performed having special
attention on: group up to the age of 12 months and group from 13 to 24 months.
In total of 2435 neoplasms found in different dog tissues, 2.26% were identified in
dogs up to age of 12 months and 4.02% in dogs aged from 13 to 24 months. In the
present analysis in the group up to 12 months 80% of tumors were benign, and 20%
were malignant followed by 67.35% benign neoplasms and 32,65% malignant in dogs
aged from 13 to 24 months. Canine cutaneous histiocytoma was the most frequent in
both of groups. Fibrosarcoma have been reported in only four months old female
Weimaraner. French bulldog and mixed-breed dogs were the most frequently affected.
These obtained data in field of veterinary oncology provide valuable epidemiological
information on neoplasms occurring in young dogs in Republic of Serbia.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023",
title = "Epidemiološka ispitivanja tumora mladih pasa u periodu od 2017. do 2022. godine u Republici Srbiji, Epidemiology of tumours in young dogs in Republic of Serbia from 2017-2022",
pages = "128-129",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3060"
}

Aleksić-Kovačević, S., Gligorić, S.,& Kukolj, V.. (2023). Epidemiološka ispitivanja tumora mladih pasa u periodu od 2017. do 2022. godine u Republici Srbiji. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 128-129.
https://hdl.handle.net/21.15107/rcub_veterinar_3060

Aleksić-Kovačević S, Gligorić S, Kukolj V. Epidemiološka ispitivanja tumora mladih pasa u periodu od 2017. do 2022. godine u Republici Srbiji. in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023. 2023;:128-129.
https://hdl.handle.net/21.15107/rcub_veterinar_3060 .

Aleksić-Kovačević, Sanja, Gligorić, Srđan, Kukolj, Vladimir, "Epidemiološka ispitivanja tumora mladih pasa u periodu od 2017. do 2022. godine u Republici Srbiji" in XXV Simpozijum epizootiologa i epidemiologa (XXV Epizootiološki dani), Novi Sad, 24 - 26. april 2023 (2023):128-129,
https://hdl.handle.net/21.15107/rcub_veterinar_3060 .

TY  - CONF
AU  - Aleksić Kovačević, Sanja
AU  - Gligorić, Srđan
AU  - Kukolj, Vladimir
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3579
AB  - Тумори паса представљају најчешћу дијагнозу у узорцима који су
достављени Лабораторији за патологију, Факултета ветеринарске
медицине, Универзитета у Београду. Учесталост неоплазми повећава
се са старењем, ипак неки тумори се чешће јављају код млађих паса
мада је доступно мало информација о појави неоплазми код паса
до 12 месеци старости.
У овој студији били су присутни различити типови бенигних и
малигних тумора код веома младих паса до 12 месеци старости.
Представљен је ретроспективни преглед хистопатолошких дијагноза
тумора у нашој лабораторији током последњих пет година (2017-
2021) код две групе младих паса: у старосној доби до 12 месеци и
паса од 13 до 24 месеца старости.
Од укупно 2435 неоплазми паса различитог ткивног порекла, 55
(2,26%) је идентификовано код паса до 12 месеци старости и 98
(4,02%) код паса старости од 13 до 24 месеца. Хистиоцитом коже
паса је био најчешће присутан тумор у обе групе младих паса: 54,55%
код паса до 12 месеци, а затим 24,49% код паса узраста 13 до 24
месеца. Неоплазме које нису припадале хистиоцитомима, груписане
су као бенигни епителни, малигни епителни, хематопоетски, бенигни
мезенхимски и малигни мезенхимски тумори. У старосној групи до 12 месеци, 80% тумора су били бенигни, а 20% малигни, а код
оних узраста 13 до 24 месеца, учесталост бенигних неоплазми
била је 67,35%, док су малигни чинили већ 32,65% свих тумора у
овој старосној категорији. Најмлађи пси у нашој студији били су
двомесечни мужјак и женка бишона са субкутаним мастоцитомом
и папиломом, међутим фибросарком је дијагностикован код женке
вајмарског птичара, старе само четири месеца. Пси расе француски
булдог и пси мешанци су најчешће били погођени неопластичним
процесима. Ови подаци дају драгоцене епидемиолошке податке о
туморима који се јављају код младих паса у Републици Србији.
AB  - Neoplasia in dogs are the most frequent diagnosis in histological submissions at Pathology Department, Faculty of Veterinary Medicine,
Belgrade University. Although incidence of neoplasms increases with
age some tumors occur more commonly in younger dogs but little
information is available on the occurrence of neoplasms in dogs up to
the age of 12 months.
In our study specimens reveal different types of benign and malignant
tumors in very young dogs up to 12 months. This is a retrospective
review of histopathological diagnoses of neoplasia at our laboratory
during last five years (2017-2021) in two groups of dogs: group up to
the age of 12 months as well as group from 13 to 24 months.
In total of 2435 neoplasms found in different dog tissues, n=55 (2.26%)
were identified in dogs up to age of 12 months and n=98 (4.02%) in
dogs aged from 13 to 24 months. Canine cutaneous histiocytoma was
the most frequent in both of groups: (n=30; 54.55%) in the dogs up
to 12 months followed by (n=24; 24.49%) in those up to 24 months.
Neoplasms other than histiocytoma were grouped as benign epithelial,
malignant epithelial, hematopoietic, benign mesenchymal and malignant
mesenchymal tumors. In the present analysis in the group up to 12
months 80% of tumors were benign, and 20% were malignant followed by 67.35% benign neoplasms and 32,65% malignant in dogs aged from
13 to 24 months. The youngest dogs in our study were two months old
Bichons male and female with subcutaneous mastocytoma and papilloma respectively, however fibrosarcoma have been reported in only
four months old female Weimaraner. French bulldog and mixed-breed
dogs were the most frequently affected. These data provide valuable
epidemiological information on neoplasms occurring in juvenile dogs
in Republic of Serbia.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022
T1  - Retrospektivna analiza zastupljenosti tumora pasa u uzrastu do 12 meseci u periodu od 2017 do 2021 u Republici Srbiji
SP  - 189
EP  - 192
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3579
ER  - 

@conference{
author = "Aleksić Kovačević, Sanja and Gligorić, Srđan and Kukolj, Vladimir",
year = "2022",
abstract = "Тумори паса представљају најчешћу дијагнозу у узорцима који су
достављени Лабораторији за патологију, Факултета ветеринарске
медицине, Универзитета у Београду. Учесталост неоплазми повећава
се са старењем, ипак неки тумори се чешће јављају код млађих паса
мада је доступно мало информација о појави неоплазми код паса
до 12 месеци старости.
У овој студији били су присутни различити типови бенигних и
малигних тумора код веома младих паса до 12 месеци старости.
Представљен је ретроспективни преглед хистопатолошких дијагноза
тумора у нашој лабораторији током последњих пет година (2017-
2021) код две групе младих паса: у старосној доби до 12 месеци и
паса од 13 до 24 месеца старости.
Од укупно 2435 неоплазми паса различитог ткивног порекла, 55
(2,26%) је идентификовано код паса до 12 месеци старости и 98
(4,02%) код паса старости од 13 до 24 месеца. Хистиоцитом коже
паса је био најчешће присутан тумор у обе групе младих паса: 54,55%
код паса до 12 месеци, а затим 24,49% код паса узраста 13 до 24
месеца. Неоплазме које нису припадале хистиоцитомима, груписане
су као бенигни епителни, малигни епителни, хематопоетски, бенигни
мезенхимски и малигни мезенхимски тумори. У старосној групи до 12 месеци, 80% тумора су били бенигни, а 20% малигни, а код
оних узраста 13 до 24 месеца, учесталост бенигних неоплазми
била је 67,35%, док су малигни чинили већ 32,65% свих тумора у
овој старосној категорији. Најмлађи пси у нашој студији били су
двомесечни мужјак и женка бишона са субкутаним мастоцитомом
и папиломом, међутим фибросарком је дијагностикован код женке
вајмарског птичара, старе само четири месеца. Пси расе француски
булдог и пси мешанци су најчешће били погођени неопластичним
процесима. Ови подаци дају драгоцене епидемиолошке податке о
туморима који се јављају код младих паса у Републици Србији., Neoplasia in dogs are the most frequent diagnosis in histological submissions at Pathology Department, Faculty of Veterinary Medicine,
Belgrade University. Although incidence of neoplasms increases with
age some tumors occur more commonly in younger dogs but little
information is available on the occurrence of neoplasms in dogs up to
the age of 12 months.
In our study specimens reveal different types of benign and malignant
tumors in very young dogs up to 12 months. This is a retrospective
review of histopathological diagnoses of neoplasia at our laboratory
during last five years (2017-2021) in two groups of dogs: group up to
the age of 12 months as well as group from 13 to 24 months.
In total of 2435 neoplasms found in different dog tissues, n=55 (2.26%)
were identified in dogs up to age of 12 months and n=98 (4.02%) in
dogs aged from 13 to 24 months. Canine cutaneous histiocytoma was
the most frequent in both of groups: (n=30; 54.55%) in the dogs up
to 12 months followed by (n=24; 24.49%) in those up to 24 months.
Neoplasms other than histiocytoma were grouped as benign epithelial,
malignant epithelial, hematopoietic, benign mesenchymal and malignant
mesenchymal tumors. In the present analysis in the group up to 12
months 80% of tumors were benign, and 20% were malignant followed by 67.35% benign neoplasms and 32,65% malignant in dogs aged from
13 to 24 months. The youngest dogs in our study were two months old
Bichons male and female with subcutaneous mastocytoma and papilloma respectively, however fibrosarcoma have been reported in only
four months old female Weimaraner. French bulldog and mixed-breed
dogs were the most frequently affected. These data provide valuable
epidemiological information on neoplasms occurring in juvenile dogs
in Republic of Serbia.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022",
title = "Retrospektivna analiza zastupljenosti tumora pasa u uzrastu do 12 meseci u periodu od 2017 do 2021 u Republici Srbiji",
pages = "189-192",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3579"
}

Aleksić Kovačević, S., Gligorić, S.,& Kukolj, V.. (2022). Retrospektivna analiza zastupljenosti tumora pasa u uzrastu do 12 meseci u periodu od 2017 do 2021 u Republici Srbiji. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022
Banja Luka : Veterinarska komora Republike Srpske., 189-192.
https://hdl.handle.net/21.15107/rcub_veterinar_3579

Aleksić Kovačević S, Gligorić S, Kukolj V. Retrospektivna analiza zastupljenosti tumora pasa u uzrastu do 12 meseci u periodu od 2017 do 2021 u Republici Srbiji. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022. 2022;:189-192.
https://hdl.handle.net/21.15107/rcub_veterinar_3579 .

Aleksić Kovačević, Sanja, Gligorić, Srđan, Kukolj, Vladimir, "Retrospektivna analiza zastupljenosti tumora pasa u uzrastu do 12 meseci u periodu od 2017 do 2021 u Republici Srbiji" in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 18. jun 2022 (2022):189-192,
https://hdl.handle.net/21.15107/rcub_veterinar_3579 .

TY  - CONF
AU  - Kukolj, Vladimir
AU  - Anicic, Milan
AU  - Marinković, Darko
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1996
AB  - Broj onkoloških slučajeva ima višegodišnji trend rasta, kako kod ljudi, tako i kod životinja, pre svega pasa. Prevencija ili dijagnostika neoplazmi u ranoj fazi bolesti u velikoj meri zavise od histološkog pre- gleda uzorka i obično su jedan od početnih koraka u postavljanju di- jagnoze. Postavljanje tačne dijagnoze, prema savremenim kriterijumi- ma za histopatološku dijagnostiku, predstavlja osnovni preduslov za sprovođenje adekvatnih terapijskih protokola, proširenje i unapređe- nje mogućnosti lečenja onkoloških pacijenata i produžavanje njiho- vog života. Histopatološka dijagnoza, pored klasifikacije i određivanja gradusa tumora, pruža kliničarima i informacije o tome da li je eksci- zija bila adekvatna, da li su margine slobodne od tumorskih ćelija, da li je tumor infiltrovao okolno tkivo i da li je započeo metastazu prodi- ranjem u krvne i/ili limfne sudove, kao i da li postoje metastaze u ko- respondentnim limfnim čvorovima, ukoliko su i oni promenjeni. Važan preduslov za postavljanje pouzdane histopatološke dijagnoze je uzor- kovanje neoplastičnog tkiva, njegovo fiksiranje i transport do laborato- rije i jasno popunjena prateća dokumentacija. Odabir tkivnog uzorka sa neadekvatne lokacije, uzorkovanje suviše velikog ili suviše malog uzorka, greške u izboru fiksativa i procesu fiksiranja, kao i nepravilno popunjen uput za ispitivanje, mogu u velikoj meri da utiču na obradu tkiva, kvalitet preparata i dobijene rezultate. Greške načinjene tokom uzorkovanja tkiva najčešće dovode do trajnih posledica i u kasnijim fa- zama obrade i naknadno teško mogu da budu otklonjene.
PB  - Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - Zbornik radova - XLII seminar inovacija znanja veterinara
T1  - Uzorkovanje neoplastičnog tkiva pasa i prateća dokumentacija
SP  - 159
EP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1996
ER  - 

@conference{
author = "Kukolj, Vladimir and Anicic, Milan and Marinković, Darko",
year = "2021",
abstract = "Broj onkoloških slučajeva ima višegodišnji trend rasta, kako kod ljudi, tako i kod životinja, pre svega pasa. Prevencija ili dijagnostika neoplazmi u ranoj fazi bolesti u velikoj meri zavise od histološkog pre- gleda uzorka i obično su jedan od početnih koraka u postavljanju di- jagnoze. Postavljanje tačne dijagnoze, prema savremenim kriterijumi- ma za histopatološku dijagnostiku, predstavlja osnovni preduslov za sprovođenje adekvatnih terapijskih protokola, proširenje i unapređe- nje mogućnosti lečenja onkoloških pacijenata i produžavanje njiho- vog života. Histopatološka dijagnoza, pored klasifikacije i određivanja gradusa tumora, pruža kliničarima i informacije o tome da li je eksci- zija bila adekvatna, da li su margine slobodne od tumorskih ćelija, da li je tumor infiltrovao okolno tkivo i da li je započeo metastazu prodi- ranjem u krvne i/ili limfne sudove, kao i da li postoje metastaze u ko- respondentnim limfnim čvorovima, ukoliko su i oni promenjeni. Važan preduslov za postavljanje pouzdane histopatološke dijagnoze je uzor- kovanje neoplastičnog tkiva, njegovo fiksiranje i transport do laborato- rije i jasno popunjena prateća dokumentacija. Odabir tkivnog uzorka sa neadekvatne lokacije, uzorkovanje suviše velikog ili suviše malog uzorka, greške u izboru fiksativa i procesu fiksiranja, kao i nepravilno popunjen uput za ispitivanje, mogu u velikoj meri da utiču na obradu tkiva, kvalitet preparata i dobijene rezultate. Greške načinjene tokom uzorkovanja tkiva najčešće dovode do trajnih posledica i u kasnijim fa- zama obrade i naknadno teško mogu da budu otklonjene.",
publisher = "Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "Zbornik radova - XLII seminar inovacija znanja veterinara",
title = "Uzorkovanje neoplastičnog tkiva pasa i prateća dokumentacija",
pages = "159-165",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1996"
}

Kukolj, V., Anicic, M.,& Marinković, D.. (2021). Uzorkovanje neoplastičnog tkiva pasa i prateća dokumentacija. in Zbornik radova - XLII seminar inovacija znanja veterinara
Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 159-165.
https://hdl.handle.net/21.15107/rcub_veterinar_1996

Kukolj V, Anicic M, Marinković D. Uzorkovanje neoplastičnog tkiva pasa i prateća dokumentacija. in Zbornik radova - XLII seminar inovacija znanja veterinara. 2021;:159-165.
https://hdl.handle.net/21.15107/rcub_veterinar_1996 .

Kukolj, Vladimir, Anicic, Milan, Marinković, Darko, "Uzorkovanje neoplastičnog tkiva pasa i prateća dokumentacija" in Zbornik radova - XLII seminar inovacija znanja veterinara (2021):159-165,
https://hdl.handle.net/21.15107/rcub_veterinar_1996 .

TY  - JOUR
AU  - Nešić, Slađan
AU  - Vučićević, Ivana
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Aničić, Milan
AU  - Ristoski, Trpe
AU  - Nikolić, Sonja
AU  - Aleksić-Kovačević, Sanja
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2075
AB  - The ageing process is accompanied by numerous changes in the brain of dogs, such as accumulation of amyloid, fibrosis of blood vessel walls and meninges, accumulation of lipofuscin, and the presence of polyglucosan bodies (PGBs), satellitosis and neuronophagia. In this study, the presence of lipofuscin and PGBs in various parts of the brain in dogs of different sexes and ages was examined. For this purpose, brain samples were stained using haematoxylin eosin, modified Ziehl Neelsen and Periodic acid Schiff (PAS) methods. Lipofuscin was visualised by Ziehl Neelsen and PAS methods of specific staining on the same brain tissue segments. Lipofuscin had accumulated in 93% of old (more than 10 years old) dog brains, mostly in neurons of the medulla oblongata. The percentage of age-related lipofuscin pigment in other examined brain tissue segments was lower than in the medulla oblongata. There was a small difference in the percentage of lipofuscin-positive individuals between the two staining methods. The presence of PGBs was established by the PAS method for the vast majority (about 93%) of the old dogs (more than 10 years old), while PGBs were not detected in the group of young dogs (up to 5 years old). However, PGBs occurred in all examined segments of the dog’s brain tissues (for each of the tissue types, from 90% to 93% of dogs were positive for PGBs). The results obtained the oldest dogs (15 years old) harboured PGBs both extracellularly and intracellularly, while in other dogs, only extracellular PGBs were seen. Lipofuscin was accumulated mostly in large neurons of olivary nuclei of the medulla oblongata. PGBs were confirmed in all examined segments of the brain tissue of dogs more than 10 years old. This is one of the numerous indications that old dogs could be a very good animal model for studying the normal ageing process or neurodegenerative diseases.
AB  - Proces starenja prate brojne promene u mozgu pasa kao što su nagomilavanje amiloida, fibroza zida krvnih sudova i moždanih ovojnica, nakupljanje lipofuscina i prisustvo poliglukoznih tela (PGB), satelitoza i neuronofagija. U ovom radu ispitivano je prisustvo lipofuscina i PGB u različitim delovima centralnog nervnog sistema kod pasa različitog pola i starosti. Uzorci mozga obojeni su hematoksilin eozinom, modifikovanom Ziehl Neelsen metodom i perjodna kiselina-Schiff (PAS) metodom. Lipofuscin je modifikovanom Ziehl Neelsen i PAS metodom specifično dokazan u istim segmentima moždanog tkiva. Dobijeni rezultati pokazuju da je lipofuscin akumuliran uglavnom u neuronima produžene moždine kod 93% pasa. Zastupljenost pigmenta u ostalim segmentima mozga bio je niži u poređenju sa produženom moždinom. Korišćenjem obe metode bojenja, ustanovljena je mala razlika u procentu pozitivnih jedinki. Prisustvo PGB dokazano je PAS metodom kod velikog broja (oko 93%) pasa eksperimentalne grupe, dok u kontrolnoj grupi njihovo prisustvo nije ustanovljeno. U svim ispitivanim segmentima moždanog tkiva kod pasa iz eksperimentalne grupe, dokazana su PGB i to od 90% do 93%. Dobijeni rezultati ukazuju da su kod najstarijih pasa iz eksperimentalne grupe PGB bila lokalizovana i van ćelije i unutar nje, a kod drugih samo ekstracelularno. Lipofuscin je akumuliran uglavnom u velikim neuronima olivarnih jedara produžene moždine. PGB su dokazana u svim ispitivanim segmentima moždanog tkiva pasa starijih od 10 godina. Ovo je jedan od brojnih dokaza da stari psi predstavljaju dobar animalni model za proučavanje normalnog procesa starenja ili neurodegenerativnih bolesti.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Histochemical characteristics and distribution of lipofuscin and polyglucosan bodies in the brain of dogs more than 10 years old
T1  - Histohemijske karakteristike i distribucija lipofuscina i poliglukozanskih tela u mozgu pasa starijih od 10 godina
VL  - 75
IS  - 1
SP  - 57
EP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2075
ER  - 

@article{
author = "Nešić, Slađan and Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Aničić, Milan and Ristoski, Trpe and Nikolić, Sonja and Aleksić-Kovačević, Sanja",
year = "2021",
abstract = "The ageing process is accompanied by numerous changes in the brain of dogs, such as accumulation of amyloid, fibrosis of blood vessel walls and meninges, accumulation of lipofuscin, and the presence of polyglucosan bodies (PGBs), satellitosis and neuronophagia. In this study, the presence of lipofuscin and PGBs in various parts of the brain in dogs of different sexes and ages was examined. For this purpose, brain samples were stained using haematoxylin eosin, modified Ziehl Neelsen and Periodic acid Schiff (PAS) methods. Lipofuscin was visualised by Ziehl Neelsen and PAS methods of specific staining on the same brain tissue segments. Lipofuscin had accumulated in 93% of old (more than 10 years old) dog brains, mostly in neurons of the medulla oblongata. The percentage of age-related lipofuscin pigment in other examined brain tissue segments was lower than in the medulla oblongata. There was a small difference in the percentage of lipofuscin-positive individuals between the two staining methods. The presence of PGBs was established by the PAS method for the vast majority (about 93%) of the old dogs (more than 10 years old), while PGBs were not detected in the group of young dogs (up to 5 years old). However, PGBs occurred in all examined segments of the dog’s brain tissues (for each of the tissue types, from 90% to 93% of dogs were positive for PGBs). The results obtained the oldest dogs (15 years old) harboured PGBs both extracellularly and intracellularly, while in other dogs, only extracellular PGBs were seen. Lipofuscin was accumulated mostly in large neurons of olivary nuclei of the medulla oblongata. PGBs were confirmed in all examined segments of the brain tissue of dogs more than 10 years old. This is one of the numerous indications that old dogs could be a very good animal model for studying the normal ageing process or neurodegenerative diseases., Proces starenja prate brojne promene u mozgu pasa kao što su nagomilavanje amiloida, fibroza zida krvnih sudova i moždanih ovojnica, nakupljanje lipofuscina i prisustvo poliglukoznih tela (PGB), satelitoza i neuronofagija. U ovom radu ispitivano je prisustvo lipofuscina i PGB u različitim delovima centralnog nervnog sistema kod pasa različitog pola i starosti. Uzorci mozga obojeni su hematoksilin eozinom, modifikovanom Ziehl Neelsen metodom i perjodna kiselina-Schiff (PAS) metodom. Lipofuscin je modifikovanom Ziehl Neelsen i PAS metodom specifično dokazan u istim segmentima moždanog tkiva. Dobijeni rezultati pokazuju da je lipofuscin akumuliran uglavnom u neuronima produžene moždine kod 93% pasa. Zastupljenost pigmenta u ostalim segmentima mozga bio je niži u poređenju sa produženom moždinom. Korišćenjem obe metode bojenja, ustanovljena je mala razlika u procentu pozitivnih jedinki. Prisustvo PGB dokazano je PAS metodom kod velikog broja (oko 93%) pasa eksperimentalne grupe, dok u kontrolnoj grupi njihovo prisustvo nije ustanovljeno. U svim ispitivanim segmentima moždanog tkiva kod pasa iz eksperimentalne grupe, dokazana su PGB i to od 90% do 93%. Dobijeni rezultati ukazuju da su kod najstarijih pasa iz eksperimentalne grupe PGB bila lokalizovana i van ćelije i unutar nje, a kod drugih samo ekstracelularno. Lipofuscin je akumuliran uglavnom u velikim neuronima olivarnih jedara produžene moždine. PGB su dokazana u svim ispitivanim segmentima moždanog tkiva pasa starijih od 10 godina. Ovo je jedan od brojnih dokaza da stari psi predstavljaju dobar animalni model za proučavanje normalnog procesa starenja ili neurodegenerativnih bolesti.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Histochemical characteristics and distribution of lipofuscin and polyglucosan bodies in the brain of dogs more than 10 years old, Histohemijske karakteristike i distribucija lipofuscina i poliglukozanskih tela u mozgu pasa starijih od 10 godina",
volume = "75",
number = "1",
pages = "57-68",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2075"
}

Nešić, S., Vučićević, I., Marinković, D., Kukolj, V., Aničić, M., Ristoski, T., Nikolić, S.,& Aleksić-Kovačević, S.. (2021). Histochemical characteristics and distribution of lipofuscin and polyglucosan bodies in the brain of dogs more than 10 years old. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 75(1), 57-68.
https://hdl.handle.net/21.15107/rcub_veterinar_2075

Nešić S, Vučićević I, Marinković D, Kukolj V, Aničić M, Ristoski T, Nikolić S, Aleksić-Kovačević S. Histochemical characteristics and distribution of lipofuscin and polyglucosan bodies in the brain of dogs more than 10 years old. in Veterinarski Glasnik. 2021;75(1):57-68.
https://hdl.handle.net/21.15107/rcub_veterinar_2075 .

Nešić, Slađan, Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Aničić, Milan, Ristoski, Trpe, Nikolić, Sonja, Aleksić-Kovačević, Sanja, "Histochemical characteristics and distribution of lipofuscin and polyglucosan bodies in the brain of dogs more than 10 years old" in Veterinarski Glasnik, 75, no. 1 (2021):57-68,
https://hdl.handle.net/21.15107/rcub_veterinar_2075 .

TY  - JOUR
AU  - Kukolj, Vladimir
AU  - Nešić, Slađan
AU  - Marinković, Darko
AU  - Aleksić-Kovačević, Sanja
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2326
AB  - Cutaneous lesions, especially skin tumors in dogs, are among the most common lesions
in this animal species. The aim of this study was to identify the most common types
of canine cutaneous lesions, to determine the absolute and relative frequency of
each type of cutaneous lesion, anatomical locations, mean age, as well as gender and
breed distribution. The examination included all samples of cutaneous lesions in dogs
obtained by surgical biopsy in veterinary clinics and examined at the Laboratory of
the Department of Pathology at the Faculty of Veterinary Medicine, University of
Belgrade from the 1st January 2011 to the 1st July 2021. In this period (126 months), a
total of 2432 samples of cutaneous lesions were examined, of which 1984 (81.58%)
were tumors (1037/1984, 52.27% benign and 947/1984, 47.73% malignant) and 448
(18.42%) non-neoplastic cutaneous lesions. The most commonly found cutaneous
tumors were: mast cell tumor (17.34% of all tumors), histiocytoma (9.78%), papilloma
(7.91%), lipoma (7.81%), squamous cell carcinoma (7.36%), trichoblastoma (4.44%),
hepatoid adenoma (4.39%) and malignant melanoma (4.18%). The most common
non-neoplastic cutaneous lesions were: follicular cyst(s) (35.04% of all non-neoplastic
lesions), pyogranulomatous chronic dermatitis (23.88%), lymphocytic dermatitis
(7.37%), hyperkeratosis (4.24%), and granulomatous dermatitis (3.79%). Our results
substantially confirm previously reported data regarding cutaneous neoplastic and nonneoplastic
lesions in dogs, and provide updated information on their frequency, animal
age, anatomic location and breed distributions.
AB  - Kožne lezije kod pasa, naročito tumori, spadaju među najčešće lezije kod ove životinjske
vrste. Cilj ovog istraživanja bio je da se identifikuju najčešći tipovi kožnih lezija
kod pasa, kao i da se utvrde apsolutna i relativna učestalost svake vrste kožnih lezija,
anatomska lokacija, prosečna starost, kao i rasna i polna distribucija. Ispitivanjem su
obuhvaćeni svi uzorci lezija kože kod pasa koji su dobijeni hirurškom biopsijom u
veterinarskim ambulantama i pregledani u laboratoriji Katedre za patologiju Fakulteta
veterinarske medicine Univerziteta u Beogradu u periodu od 1. januara 2011. do 1.
jula 2021. godine. U ovom periodu od 126 meseci, pregledana su ukupno 2432 uzorka
kožnih lezija, od čega su 1984 (81,58%) bili tumori (1037/1984, 52,27% benigni i
947/1984, 47,73% maligni) i 448 (18,42%) neneoplastične lezije kože. Najčešći tumori
kože bili su: mastocitom (17,34% svih tumora), histiocitom (9,78%), papilom (7,91%),
lipom (7,81%), planocelularni karcinom (7,36%), trihoblastom (4,44%), adenom hepatoidne
žlezde (4,39%) i maligni melanom (4,18%). Najčešće neneoplastične lezije
kože bile su: folikularne ciste (35,04% svih neneoplastičnih lezija), hronični piogranulomatozni
dermatitis (23,88%), limfocitni dermatitis (7,37%), hiperkeratoza (4,24%)
i granulomatozni dermatitis (3,79%). Naši rezultati u velikoj meri potvrđuju ranije
objavljene podatke o neoplastičnim i neneoplastičnim lezijama kože kod pasa i pružaju
nove ažurirane informacije o njihovoj učestalosti, starosti životinje, anatomskoj lokaciji
i rasnoj i polnoj distribuciji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria-Beograd
T1  - Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: A retrospective study of 2432 cases (2011 - mid 2021)
T1  - Prevalenca i distribucija neoplastičnih i neneoplastičnih lezija kože kod pasa u Srbiji: Retrospektivna studija 2432 slučaja (2011 - sredina 2021)
VL  - 71
IS  - 4
SP  - 403
EP  - 416
DO  - 10.2478/acve-2021-0034
ER  - 

@article{
author = "Kukolj, Vladimir and Nešić, Slađan and Marinković, Darko and Aleksić-Kovačević, Sanja",
year = "2021",
abstract = "Cutaneous lesions, especially skin tumors in dogs, are among the most common lesions
in this animal species. The aim of this study was to identify the most common types
of canine cutaneous lesions, to determine the absolute and relative frequency of
each type of cutaneous lesion, anatomical locations, mean age, as well as gender and
breed distribution. The examination included all samples of cutaneous lesions in dogs
obtained by surgical biopsy in veterinary clinics and examined at the Laboratory of
the Department of Pathology at the Faculty of Veterinary Medicine, University of
Belgrade from the 1st January 2011 to the 1st July 2021. In this period (126 months), a
total of 2432 samples of cutaneous lesions were examined, of which 1984 (81.58%)
were tumors (1037/1984, 52.27% benign and 947/1984, 47.73% malignant) and 448
(18.42%) non-neoplastic cutaneous lesions. The most commonly found cutaneous
tumors were: mast cell tumor (17.34% of all tumors), histiocytoma (9.78%), papilloma
(7.91%), lipoma (7.81%), squamous cell carcinoma (7.36%), trichoblastoma (4.44%),
hepatoid adenoma (4.39%) and malignant melanoma (4.18%). The most common
non-neoplastic cutaneous lesions were: follicular cyst(s) (35.04% of all non-neoplastic
lesions), pyogranulomatous chronic dermatitis (23.88%), lymphocytic dermatitis
(7.37%), hyperkeratosis (4.24%), and granulomatous dermatitis (3.79%). Our results
substantially confirm previously reported data regarding cutaneous neoplastic and nonneoplastic
lesions in dogs, and provide updated information on their frequency, animal
age, anatomic location and breed distributions., Kožne lezije kod pasa, naročito tumori, spadaju među najčešće lezije kod ove životinjske
vrste. Cilj ovog istraživanja bio je da se identifikuju najčešći tipovi kožnih lezija
kod pasa, kao i da se utvrde apsolutna i relativna učestalost svake vrste kožnih lezija,
anatomska lokacija, prosečna starost, kao i rasna i polna distribucija. Ispitivanjem su
obuhvaćeni svi uzorci lezija kože kod pasa koji su dobijeni hirurškom biopsijom u
veterinarskim ambulantama i pregledani u laboratoriji Katedre za patologiju Fakulteta
veterinarske medicine Univerziteta u Beogradu u periodu od 1. januara 2011. do 1.
jula 2021. godine. U ovom periodu od 126 meseci, pregledana su ukupno 2432 uzorka
kožnih lezija, od čega su 1984 (81,58%) bili tumori (1037/1984, 52,27% benigni i
947/1984, 47,73% maligni) i 448 (18,42%) neneoplastične lezije kože. Najčešći tumori
kože bili su: mastocitom (17,34% svih tumora), histiocitom (9,78%), papilom (7,91%),
lipom (7,81%), planocelularni karcinom (7,36%), trihoblastom (4,44%), adenom hepatoidne
žlezde (4,39%) i maligni melanom (4,18%). Najčešće neneoplastične lezije
kože bile su: folikularne ciste (35,04% svih neneoplastičnih lezija), hronični piogranulomatozni
dermatitis (23,88%), limfocitni dermatitis (7,37%), hiperkeratoza (4,24%)
i granulomatozni dermatitis (3,79%). Naši rezultati u velikoj meri potvrđuju ranije
objavljene podatke o neoplastičnim i neneoplastičnim lezijama kože kod pasa i pružaju
nove ažurirane informacije o njihovoj učestalosti, starosti životinje, anatomskoj lokaciji
i rasnoj i polnoj distribuciji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria-Beograd",
title = "Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: A retrospective study of 2432 cases (2011 - mid 2021), Prevalenca i distribucija neoplastičnih i neneoplastičnih lezija kože kod pasa u Srbiji: Retrospektivna studija 2432 slučaja (2011 - sredina 2021)",
volume = "71",
number = "4",
pages = "403-416",
doi = "10.2478/acve-2021-0034"
}

Kukolj, V., Nešić, S., Marinković, D.,& Aleksić-Kovačević, S.. (2021). Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: A retrospective study of 2432 cases (2011 - mid 2021). in Acta veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(4), 403-416.
https://doi.org/10.2478/acve-2021-0034

Kukolj V, Nešić S, Marinković D, Aleksić-Kovačević S. Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: A retrospective study of 2432 cases (2011 - mid 2021). in Acta veterinaria-Beograd. 2021;71(4):403-416.
doi:10.2478/acve-2021-0034 .

Kukolj, Vladimir, Nešić, Slađan, Marinković, Darko, Aleksić-Kovačević, Sanja, "Prevalence and distribution of canine neoplastic and non-neoplastic cutaneous lesions in Serbia: A retrospective study of 2432 cases (2011 - mid 2021)" in Acta veterinaria-Beograd, 71, no. 4 (2021):403-416,
https://doi.org/10.2478/acve-2021-0034 . .

TY  - JOUR
AU  - Kureljušić, Branislav
AU  - Aleksić-Kovačević, Sanja
AU  - Savić, Božidar
AU  - Prodanović, Radiša
AU  - Jezdimirović, Nemanja
AU  - Milićević, Vesna
AU  - Maksimović Zorić, Jelena
AU  - Kureljušić, Jasna
AU  - Žutić, Jadranka
AU  - Knežević, Đorđe
AU  - Spalević, Ljiljana
AU  - Kukolj, Vladimir
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2784
AB  - Hepatitis E virus (HEV), the zoonotic agent of infectious hepatitis, is present in swine farms
in different geographical areas. Little is known about the mechanism of liver damage and
type of local immune response by HEV in swine. Therefore, the aim of this study was to
determine the morphological and immunophenotypic characteristics of hepatic lesions
caused by hepatitis E virus in naturally infected swine. In this study, liver samples of 12
slaughtered 10 weeks old pigs which were RT‑PCR positive for HEV RNA in rectal swab
samples have been used. Livers were macroscopically examined and samples were taken
for histopathological, immunohistochemical (CD3, CD79α and TGF‑β1), semiquantitative,
morphometric analysis, RT‑nested‑PCR, PCR and bacteriological analysis. Microscopically,
mild and moderate multifocal lymphoplasmacytic hepatitis was observed. Apoptotic bodies
were observed as areas of focal eosinophilic condensation in the cytoplasm of 33.33% liver
samples, while in 16.67% liver samples portal fibrosis was detected. Immunohistochemically,
portal and lobular lymphocytes in the mononuclear liver infiltrate were predominantly
CD3+ T cells (234.80 ± 79.98). An intense TGF‑β1 positive reaction was observed within the
mononuclear cell infiltrate as well as polymorphonuclear cells in liver samples with apoptosis
of hepatocytes. In all 12 tested liver samples HEV RNA was detected by RT‑nested‑PCR.
HEV is noncytopathic, and this finding provides further evidence for an immune mediated
pathogenesis in hepatitis E virus infection in swine. Also, the role of CD3+ cells in hepatocyte
damage is clearly demonstrated.
PB  - Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)
T2  - Veterinaria Italiana
T1  - Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus
VL  - 54
IS  - 7
SP  - 287
EP  - 295
DO  - 10.12834/VetIt.1813.9553.3
ER  - 

@article{
author = "Kureljušić, Branislav and Aleksić-Kovačević, Sanja and Savić, Božidar and Prodanović, Radiša and Jezdimirović, Nemanja and Milićević, Vesna and Maksimović Zorić, Jelena and Kureljušić, Jasna and Žutić, Jadranka and Knežević, Đorđe and Spalević, Ljiljana and Kukolj, Vladimir",
year = "2021",
abstract = "Hepatitis E virus (HEV), the zoonotic agent of infectious hepatitis, is present in swine farms
in different geographical areas. Little is known about the mechanism of liver damage and
type of local immune response by HEV in swine. Therefore, the aim of this study was to
determine the morphological and immunophenotypic characteristics of hepatic lesions
caused by hepatitis E virus in naturally infected swine. In this study, liver samples of 12
slaughtered 10 weeks old pigs which were RT‑PCR positive for HEV RNA in rectal swab
samples have been used. Livers were macroscopically examined and samples were taken
for histopathological, immunohistochemical (CD3, CD79α and TGF‑β1), semiquantitative,
morphometric analysis, RT‑nested‑PCR, PCR and bacteriological analysis. Microscopically,
mild and moderate multifocal lymphoplasmacytic hepatitis was observed. Apoptotic bodies
were observed as areas of focal eosinophilic condensation in the cytoplasm of 33.33% liver
samples, while in 16.67% liver samples portal fibrosis was detected. Immunohistochemically,
portal and lobular lymphocytes in the mononuclear liver infiltrate were predominantly
CD3+ T cells (234.80 ± 79.98). An intense TGF‑β1 positive reaction was observed within the
mononuclear cell infiltrate as well as polymorphonuclear cells in liver samples with apoptosis
of hepatocytes. In all 12 tested liver samples HEV RNA was detected by RT‑nested‑PCR.
HEV is noncytopathic, and this finding provides further evidence for an immune mediated
pathogenesis in hepatitis E virus infection in swine. Also, the role of CD3+ cells in hepatocyte
damage is clearly demonstrated.",
publisher = "Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)",
journal = "Veterinaria Italiana",
title = "Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus",
volume = "54",
number = "7",
pages = "287-295",
doi = "10.12834/VetIt.1813.9553.3"
}

Kureljušić, B., Aleksić-Kovačević, S., Savić, B., Prodanović, R., Jezdimirović, N., Milićević, V., Maksimović Zorić, J., Kureljušić, J., Žutić, J., Knežević, Đ., Spalević, L.,& Kukolj, V.. (2021). Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus. in Veterinaria Italiana
Istituto Zooprofilattico Sperimentale dell’Abruzzo e del Molise ‘G. Caporale’ (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise)., 54(7), 287-295.
https://doi.org/10.12834/VetIt.1813.9553.3

Kureljušić B, Aleksić-Kovačević S, Savić B, Prodanović R, Jezdimirović N, Milićević V, Maksimović Zorić J, Kureljušić J, Žutić J, Knežević Đ, Spalević L, Kukolj V. Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus. in Veterinaria Italiana. 2021;54(7):287-295.
doi:10.12834/VetIt.1813.9553.3 .

Kureljušić, Branislav, Aleksić-Kovačević, Sanja, Savić, Božidar, Prodanović, Radiša, Jezdimirović, Nemanja, Milićević, Vesna, Maksimović Zorić, Jelena, Kureljušić, Jasna, Žutić, Jadranka, Knežević, Đorđe, Spalević, Ljiljana, Kukolj, Vladimir, "Morphological and immunophenotypic characteristics of the liver of swine naturally infected with hepatitis E virus" in Veterinaria Italiana, 54, no. 7 (2021):287-295,
https://doi.org/10.12834/VetIt.1813.9553.3 . .

TY  - JOUR
AU  - Drašković, Vladimir
AU  - Stanimirović, Zoran
AU  - Glišić, Milica
AU  - Bošnjak-Neumuller, Jasna
AU  - Teodorović, Radislava
AU  - Teodorović, Vlado
AU  - Kukolj, Vladimir
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1810
AB  - Proliferative enteropathy, also known as proliferative ileitis, is considered to be one of the most economically important diseases in pig production worldwide. The estimated losses per affected growing pig usually range from US $1 to $5. The disease is caused by Lawsonia intrawellularis, a Gram-negative, obligately intracellular bacterium. Control of the disease can be achieved with the use of vaccines or antibiotics. Recently there has been an increase in the efforts in the control of certain pathologies of the digestive system with phytogenic additives. The aim of this work was to assess the effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical infection with L. intracellularis acquired spontaneously. Histomorphometry analysis showed that crypt depth was significantly shorter (P <0.05), and the villus-height-to-crypt-depth ratio (P<0.05) significantly greater in the treatment group than the control. This improvement in the histological parameters of the intestine, considered to be indicators of its health, proved the positive effect of the tested additive on the digestive system in pigs.
AB  - Proliferativna enteropatija poznata kao i proliferativni ileitis smatra se jednom od ekonomski najznačajnih bolesti u svinjarskoj proizvodnji širom sveta. Procenjeni gubici
po obolelom tovljeniku obično se kreću od 1 do 5 američkih dolara. Uzročnik ovog
oboljenja je Lawsonia intracellularis, gram negativna obligatna intracelularna bakterija.
Kontrola ovog oboljenja ostvaruje se primenom vakcinacije ili antibiotika. U poslednje
vreme sve je češća primena fi togenih aditiva u cilju kontolisanja određenih patoloških
stanja digestivnog trakta. Cilj ovog rada bio je da se ustanove efekti fi togenog aditiva na
histomorfometrijske karakteristike creva kod odlučenih prasadi supklinički prirodno
infi ciranih L. intracellularis. Histomorfometrijska analiza pokazala je da su kripte bile
značajno (P<0,05) pliće, a količnik visine vilusa i dubine kripti značajno veći (P<0,05)
u tretiranoj grupi prasadi nego u kontroli. Ovo poboljšanje histoloških parametara
creva, koje se smatraju indikatorima njegovog zdravlja, dokaz je pozitivnog efekta ispitivanog aditiva na digestivni sistem svinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical natural infection with lawsonia intracellularis
T1  - Efekti fitogenog aditiva na histomorfometrijske karakteristike creva kod odlučene prasadi supklinički prirodno inficirane bakterijom lawsonia intracellularis
VL  - 70
IS  - 1
SP  - 81
EP  - 91
DO  - 10.2478/acve-2020-0006
ER  - 

@article{
author = "Drašković, Vladimir and Stanimirović, Zoran and Glišić, Milica and Bošnjak-Neumuller, Jasna and Teodorović, Radislava and Teodorović, Vlado and Kukolj, Vladimir",
year = "2020",
abstract = "Proliferative enteropathy, also known as proliferative ileitis, is considered to be one of the most economically important diseases in pig production worldwide. The estimated losses per affected growing pig usually range from US $1 to $5. The disease is caused by Lawsonia intrawellularis, a Gram-negative, obligately intracellular bacterium. Control of the disease can be achieved with the use of vaccines or antibiotics. Recently there has been an increase in the efforts in the control of certain pathologies of the digestive system with phytogenic additives. The aim of this work was to assess the effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical infection with L. intracellularis acquired spontaneously. Histomorphometry analysis showed that crypt depth was significantly shorter (P <0.05), and the villus-height-to-crypt-depth ratio (P<0.05) significantly greater in the treatment group than the control. This improvement in the histological parameters of the intestine, considered to be indicators of its health, proved the positive effect of the tested additive on the digestive system in pigs., Proliferativna enteropatija poznata kao i proliferativni ileitis smatra se jednom od ekonomski najznačajnih bolesti u svinjarskoj proizvodnji širom sveta. Procenjeni gubici
po obolelom tovljeniku obično se kreću od 1 do 5 američkih dolara. Uzročnik ovog
oboljenja je Lawsonia intracellularis, gram negativna obligatna intracelularna bakterija.
Kontrola ovog oboljenja ostvaruje se primenom vakcinacije ili antibiotika. U poslednje
vreme sve je češća primena fi togenih aditiva u cilju kontolisanja određenih patoloških
stanja digestivnog trakta. Cilj ovog rada bio je da se ustanove efekti fi togenog aditiva na
histomorfometrijske karakteristike creva kod odlučenih prasadi supklinički prirodno
infi ciranih L. intracellularis. Histomorfometrijska analiza pokazala je da su kripte bile
značajno (P<0,05) pliće, a količnik visine vilusa i dubine kripti značajno veći (P<0,05)
u tretiranoj grupi prasadi nego u kontroli. Ovo poboljšanje histoloških parametara
creva, koje se smatraju indikatorima njegovog zdravlja, dokaz je pozitivnog efekta ispitivanog aditiva na digestivni sistem svinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical natural infection with lawsonia intracellularis, Efekti fitogenog aditiva na histomorfometrijske karakteristike creva kod odlučene prasadi supklinički prirodno inficirane bakterijom lawsonia intracellularis",
volume = "70",
number = "1",
pages = "81-91",
doi = "10.2478/acve-2020-0006"
}

Drašković, V., Stanimirović, Z., Glišić, M., Bošnjak-Neumuller, J., Teodorović, R., Teodorović, V.,& Kukolj, V.. (2020). The effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical natural infection with lawsonia intracellularis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(1), 81-91.
https://doi.org/10.2478/acve-2020-0006

Drašković V, Stanimirović Z, Glišić M, Bošnjak-Neumuller J, Teodorović R, Teodorović V, Kukolj V. The effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical natural infection with lawsonia intracellularis. in Acta Veterinaria-Beograd. 2020;70(1):81-91.
doi:10.2478/acve-2020-0006 .

Drašković, Vladimir, Stanimirović, Zoran, Glišić, Milica, Bošnjak-Neumuller, Jasna, Teodorović, Radislava, Teodorović, Vlado, Kukolj, Vladimir, "The effects of a phytogenic additive on the histomorphometric characteristics of the intestines in weaned pigs with a subclinical natural infection with lawsonia intracellularis" in Acta Veterinaria-Beograd, 70, no. 1 (2020):81-91,
https://doi.org/10.2478/acve-2020-0006 . .

TY  - JOUR
AU  - Nešić, Slađan
AU  - Andrić, Nenad
AU  - Vučićević, Ivana
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Aničić, Milan
AU  - Aleksić-Kovačević, Sanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1876
PB  - Elsevier Sci Ltd, Oxford
T2  - Journal of Comparative Pathology
T1  - Characteristic Histopathological Features of Necrotizing Encephalitis and Syringomyelia in the Central Nervous System of a Yorkshire Terrier
VL  - 174
SP  - 168
DO  - 10.1016/j.jcpa.2019.10.091
ER  - 

@article{
author = "Nešić, Slađan and Andrić, Nenad and Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Aničić, Milan and Aleksić-Kovačević, Sanja",
year = "2020",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Journal of Comparative Pathology",
title = "Characteristic Histopathological Features of Necrotizing Encephalitis and Syringomyelia in the Central Nervous System of a Yorkshire Terrier",
volume = "174",
pages = "168",
doi = "10.1016/j.jcpa.2019.10.091"
}

Nešić, S., Andrić, N., Vučićević, I., Marinković, D., Kukolj, V., Aničić, M.,& Aleksić-Kovačević, S.. (2020). Characteristic Histopathological Features of Necrotizing Encephalitis and Syringomyelia in the Central Nervous System of a Yorkshire Terrier. in Journal of Comparative Pathology
Elsevier Sci Ltd, Oxford., 174, 168.
https://doi.org/10.1016/j.jcpa.2019.10.091

Nešić S, Andrić N, Vučićević I, Marinković D, Kukolj V, Aničić M, Aleksić-Kovačević S. Characteristic Histopathological Features of Necrotizing Encephalitis and Syringomyelia in the Central Nervous System of a Yorkshire Terrier. in Journal of Comparative Pathology. 2020;174:168.
doi:10.1016/j.jcpa.2019.10.091 .

Nešić, Slađan, Andrić, Nenad, Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Aničić, Milan, Aleksić-Kovačević, Sanja, "Characteristic Histopathological Features of Necrotizing Encephalitis and Syringomyelia in the Central Nervous System of a Yorkshire Terrier" in Journal of Comparative Pathology, 174 (2020):168,
https://doi.org/10.1016/j.jcpa.2019.10.091 . .

TY  - CONF
AU  - Vučićević, Ivana
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Nešić, Slađan
AU  - Aničić, Milan
AU  - Aleksić-Kovačević, Sanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2908
AB  - Transmisivne spongiformne encefalopatije (TSE) su neurodegenerativna oboljenja životinja i ljudi koja se karakterišu nakupljanjem izmenjenog oblika prionskog proteina (PrPres) u mozgu. Asimptomatski
period može trajati i nekoliko godina, ali je bolest uvek sa smrtnim ishodom. Monitoring TSE obuhvata ispitivanje prisustva PrPres kod goveda, a zatim i kod ovaca i koza, kod kojih uzrokuje oboljenje pod nazivom “ovčija svrabež”. U novije vreme se, u većini zemalja EU, naročita pažnja poklanja prisustvu PrPres kod jelenske divljači i pojavi hronične slabosti jelena. Spongiformna encefalopatija goveda i “ovčija
svrabež” se javljaju u klasičnoj i atipičnoj formi, pri čemu se smatra da
atipična forma nastaje spontano i da nije kontagiozna. Na osnovu OIE
podele, razlikuju se dve kategorije statusa u pogledu prisustva bovine
spongiformne encefalopatije (BSE). Od 2019. godine, Republika Srbija je stekla status zemlje sa zanemarljivim rizikom. Radi održavanja
ovog statusa zemlje, potrebno je od svih propisanih vrsta i kategorija životinja vršiti pravilno uzorkovanje i ispitivanje tkiva. Za analizu se
uzorkuje deo produžene moždine u nivou obex-a, odnosno ceo mozak kod klinički sumnjivih slučajeva. Uzorkovanje produžene moždine, bez otvaranja lobanje, obavlja se upotrebom instrumenta u obliku kašike sa oštrim ivicama. Greške prilikom uzorkovanja, pakovanja,
slanja i obeležavanja uzorkovanog materijala mogu ugroziti verodostojnost rezultata laboratorijske analize.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLI Seminar za inovacije znanja veterinara, Beograd, 2020
T1  - Uzorkovanje tkiva za dijagnostiku TSE u zemlji sa statusom zanemarljivog rizika
SP  - 77
EP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2908
ER  - 

@conference{
author = "Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Nešić, Slađan and Aničić, Milan and Aleksić-Kovačević, Sanja",
year = "2020",
abstract = "Transmisivne spongiformne encefalopatije (TSE) su neurodegenerativna oboljenja životinja i ljudi koja se karakterišu nakupljanjem izmenjenog oblika prionskog proteina (PrPres) u mozgu. Asimptomatski
period može trajati i nekoliko godina, ali je bolest uvek sa smrtnim ishodom. Monitoring TSE obuhvata ispitivanje prisustva PrPres kod goveda, a zatim i kod ovaca i koza, kod kojih uzrokuje oboljenje pod nazivom “ovčija svrabež”. U novije vreme se, u većini zemalja EU, naročita pažnja poklanja prisustvu PrPres kod jelenske divljači i pojavi hronične slabosti jelena. Spongiformna encefalopatija goveda i “ovčija
svrabež” se javljaju u klasičnoj i atipičnoj formi, pri čemu se smatra da
atipična forma nastaje spontano i da nije kontagiozna. Na osnovu OIE
podele, razlikuju se dve kategorije statusa u pogledu prisustva bovine
spongiformne encefalopatije (BSE). Od 2019. godine, Republika Srbija je stekla status zemlje sa zanemarljivim rizikom. Radi održavanja
ovog statusa zemlje, potrebno je od svih propisanih vrsta i kategorija životinja vršiti pravilno uzorkovanje i ispitivanje tkiva. Za analizu se
uzorkuje deo produžene moždine u nivou obex-a, odnosno ceo mozak kod klinički sumnjivih slučajeva. Uzorkovanje produžene moždine, bez otvaranja lobanje, obavlja se upotrebom instrumenta u obliku kašike sa oštrim ivicama. Greške prilikom uzorkovanja, pakovanja,
slanja i obeležavanja uzorkovanog materijala mogu ugroziti verodostojnost rezultata laboratorijske analize.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLI Seminar za inovacije znanja veterinara, Beograd, 2020",
title = "Uzorkovanje tkiva za dijagnostiku TSE u zemlji sa statusom zanemarljivog rizika",
pages = "77-86",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2908"
}

Vučićević, I., Marinković, D., Kukolj, V., Nešić, S., Aničić, M.,& Aleksić-Kovačević, S.. (2020). Uzorkovanje tkiva za dijagnostiku TSE u zemlji sa statusom zanemarljivog rizika. in XLI Seminar za inovacije znanja veterinara, Beograd, 2020
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 77-86.
https://hdl.handle.net/21.15107/rcub_veterinar_2908

Vučićević I, Marinković D, Kukolj V, Nešić S, Aničić M, Aleksić-Kovačević S. Uzorkovanje tkiva za dijagnostiku TSE u zemlji sa statusom zanemarljivog rizika. in XLI Seminar za inovacije znanja veterinara, Beograd, 2020. 2020;:77-86.
https://hdl.handle.net/21.15107/rcub_veterinar_2908 .

Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Nešić, Slađan, Aničić, Milan, Aleksić-Kovačević, Sanja, "Uzorkovanje tkiva za dijagnostiku TSE u zemlji sa statusom zanemarljivog rizika" in XLI Seminar za inovacije znanja veterinara, Beograd, 2020 (2020):77-86,
https://hdl.handle.net/21.15107/rcub_veterinar_2908 .

TY  - JOUR
AU  - Vučićević, Ivana
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Nešić, Slađan
AU  - Aničić, Milan
AU  - Durdević, Biljana
AU  - Aleksić-Kovačević, Sanja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1769
AB  - Peripheral nerve sheath tumors (PNSTs) comprise a heterogeneous group of neoplasms originating from the elements of the nerve sheath. They are divided into two forms: benign and malignant PNST. Both benign and malignant PNSTs are not very common in domestic animals but they are reported in different animal species. Histologically, PNSTs are composed predominantly of spindle cells arranged in bundles, whorls and sheets, with a different number of pleomorphic cells and mitotic figures. The aim of this study was a reclassification of 17 dog tumor samples initially diagnosed with peripheral nerve sheath tumors using histopathological analysis. The main criterion for reclassification was immunohistochemical positivity for various antigens. PNSTs are often histologically very similar to other spindle cell tumors and immunohistochemistry is required for differential diagnosis. PNSTs generally express vimentin, S-100 protein, glial fibrillary acidic protein (GFAP), collagen IV and laminin. Four tumor samples were positive to muscular marker alpha-SMA and vimentin and negative for S-100 protein and desmin. The spindle cells whirling around some blood vessels were observed in these tumors so they were reclassified as perivascular wall tumors (PWTs). The other 13 tumors were S-100 protein and vimentin positive and alpha-SMA and desmin negative, thus classified as PNST. The use of the immunohistochemical panel is necessary for distinguishing PNSTs from PWTs in routine diagnostics.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Immunohistochemical distinguishing between canine peripheral nerve sheath tumors and perivascular wall tumors
VL  - 69
IS  - 3
SP  - 290
EP  - 299
DO  - 10.2478/acve-2019-0024
ER  - 

@article{
author = "Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Nešić, Slađan and Aničić, Milan and Durdević, Biljana and Aleksić-Kovačević, Sanja",
year = "2019",
abstract = "Peripheral nerve sheath tumors (PNSTs) comprise a heterogeneous group of neoplasms originating from the elements of the nerve sheath. They are divided into two forms: benign and malignant PNST. Both benign and malignant PNSTs are not very common in domestic animals but they are reported in different animal species. Histologically, PNSTs are composed predominantly of spindle cells arranged in bundles, whorls and sheets, with a different number of pleomorphic cells and mitotic figures. The aim of this study was a reclassification of 17 dog tumor samples initially diagnosed with peripheral nerve sheath tumors using histopathological analysis. The main criterion for reclassification was immunohistochemical positivity for various antigens. PNSTs are often histologically very similar to other spindle cell tumors and immunohistochemistry is required for differential diagnosis. PNSTs generally express vimentin, S-100 protein, glial fibrillary acidic protein (GFAP), collagen IV and laminin. Four tumor samples were positive to muscular marker alpha-SMA and vimentin and negative for S-100 protein and desmin. The spindle cells whirling around some blood vessels were observed in these tumors so they were reclassified as perivascular wall tumors (PWTs). The other 13 tumors were S-100 protein and vimentin positive and alpha-SMA and desmin negative, thus classified as PNST. The use of the immunohistochemical panel is necessary for distinguishing PNSTs from PWTs in routine diagnostics.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Immunohistochemical distinguishing between canine peripheral nerve sheath tumors and perivascular wall tumors",
volume = "69",
number = "3",
pages = "290-299",
doi = "10.2478/acve-2019-0024"
}

Vučićević, I., Marinković, D., Kukolj, V., Nešić, S., Aničić, M., Durdević, B.,& Aleksić-Kovačević, S.. (2019). Immunohistochemical distinguishing between canine peripheral nerve sheath tumors and perivascular wall tumors. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3), 290-299.
https://doi.org/10.2478/acve-2019-0024

Vučićević I, Marinković D, Kukolj V, Nešić S, Aničić M, Durdević B, Aleksić-Kovačević S. Immunohistochemical distinguishing between canine peripheral nerve sheath tumors and perivascular wall tumors. in Acta Veterinaria-Beograd. 2019;69(3):290-299.
doi:10.2478/acve-2019-0024 .

Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Nešić, Slađan, Aničić, Milan, Durdević, Biljana, Aleksić-Kovačević, Sanja, "Immunohistochemical distinguishing between canine peripheral nerve sheath tumors and perivascular wall tumors" in Acta Veterinaria-Beograd, 69, no. 3 (2019):290-299,
https://doi.org/10.2478/acve-2019-0024 . .

TY  - CONF
AU  - Nešić, Slađan
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Vučićević, Ivana
AU  - Aničić, Milan
AU  - Aleksić-Kovačević, Sanja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3053
AB  - Клостридијалне инфекције животиња проузроковане су анаеробним
бактеријама из рода Clostridium. Клостридије су у спољашњој средини широко
распрострањене и веома отпорне бактерије, јер у неповољним условима
стварају споре које могу дуго да преживе. Такође, оне су нормални становници
дигестивног система животиња и људи. Код здравих животиња најчешће долази
до појаве инфекције приликом промене режима исхране, код повреда или
слабљења имунитета. Постоји велики број различитих врста бактерија из рода
Clostridium, али нису сви проузроковачи болести. Патогене врсте клостридија
проузрокују поремећаје здравственог стања животиња, најчешће са фаталним
исходом. Стопа морталитета код клостридијалних инфекција је висока. Због те
чињенице у фармском одгоју животиња могу да настану велики економски
губици. Најзначајније патогене врсте бактерија из рода Clostridium су: Cl.
perfrigens, Cl. chauvoei, Cl. haemoliticum, Cl. septicum, Cl. novyi, Cl. piliformis, Cl.
tetani и Cl. botulinum. Већина патогених сојева може самостално да проузрокује
настанак болести, али у неким случајевима то су мешовите инфекције
различитим врстама клостридија. Патогено дејство клостридије остварују након
умножавања и ослобађања различитих типова токсина, који могу да
проузрокују крвављења и некротичне промене локално или системски. У неким
случајевима долази до накупљања гасова у ткивима и органима. Бактеријски
токсини могу да изазову ћелијску смрт индиректно, смањењем оксигенације
ткива, или директно, оштећењем мембране ћелија у ткивима. Животиње
угињавају услед масивних некроза и крвављења праћених запаљењем у ткивима
и органима. У зависности од проузроковача некротично-хеморагичне промене
и запаљења могу да настану најчешће у дигестивном систему, јетри, централном
нервном систему, срцу и скелетној мускулатури различитих животињских
врста. Сваки тип клостридијалних токсина утиче на појаву карактеристичних
морфолошких промена код појединих животињских врста.
AB  - Clostridial infections in animals are caused by anaerobic bacteria of the genus
Clostridium. Clostridium species are widely distributed in environment and highly
resistant bacteria, due to their ability to form a spore and survive for a long time. They
represent residential microorganisms in the gastrointestinal tract of animals and
humans. In healthy animals, change in diet, injuries or weakened immune system most
commonly trigger the onset clostridial infections. There is a large number of different
species of genus Clostridium, but not all species of clostridia cause disease.
Pathogenic species of clostridia cause animal health disorders, most often with a fatal
outcome. The rate of mortality in clostridial infections is high. Due to this fact in the
farm breeding of animals, large economic losses can occur. The most important
pathogenic bacteria of the genus Clostridium are: Cl. perfrigens, Cl. chauvoei, Cl.
haemoliticum, Cl. septicum, Cl. novyi, Cl. pyliformis, Cl. tetani and Cl. botulinum.
Most commonly, infection is caused by single bacterial species, but sometimes it is
caused by variety of pathogenic clostridial species. The pathogenic effect of clostridia
occurs after the multiplication and release of different types of toxins, which can cause
local or systemic bleeding and necrotic changes. In some cases, gases accumulate in
the tissues and organs. The bacterial toxins can cause cell death indirectly, by reducing
tissue oxygenation, or directly by damaging plasma membrane of cells in tissues.
Animals could die due to massive necrosis and bleeding accompanied by
inflammation in tissues and organs. Depending on the causative factor, necrotichemorrhagic changes and inflammation can occur most often in the gastrointestinal
tract, liver, central nervous system, heart and skeletal muscle of many animal species.
Each type of clostridial toxins cause the characteristic morphological changes in
certain animal species.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
T1  - Morfološke manifestacije klostridijalnih infekcija kod različitih životinjskih vrsta
T1  - Morphological changes due to clostridial infections in different animal species
SP  - 158
EP  - 159
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3053
ER  - 

@conference{
author = "Nešić, Slađan and Marinković, Darko and Kukolj, Vladimir and Vučićević, Ivana and Aničić, Milan and Aleksić-Kovačević, Sanja",
year = "2019",
abstract = "Клостридијалне инфекције животиња проузроковане су анаеробним
бактеријама из рода Clostridium. Клостридије су у спољашњој средини широко
распрострањене и веома отпорне бактерије, јер у неповољним условима
стварају споре које могу дуго да преживе. Такође, оне су нормални становници
дигестивног система животиња и људи. Код здравих животиња најчешће долази
до појаве инфекције приликом промене режима исхране, код повреда или
слабљења имунитета. Постоји велики број различитих врста бактерија из рода
Clostridium, али нису сви проузроковачи болести. Патогене врсте клостридија
проузрокују поремећаје здравственог стања животиња, најчешће са фаталним
исходом. Стопа морталитета код клостридијалних инфекција је висока. Због те
чињенице у фармском одгоју животиња могу да настану велики економски
губици. Најзначајније патогене врсте бактерија из рода Clostridium су: Cl.
perfrigens, Cl. chauvoei, Cl. haemoliticum, Cl. septicum, Cl. novyi, Cl. piliformis, Cl.
tetani и Cl. botulinum. Већина патогених сојева може самостално да проузрокује
настанак болести, али у неким случајевима то су мешовите инфекције
различитим врстама клостридија. Патогено дејство клостридије остварују након
умножавања и ослобађања различитих типова токсина, који могу да
проузрокују крвављења и некротичне промене локално или системски. У неким
случајевима долази до накупљања гасова у ткивима и органима. Бактеријски
токсини могу да изазову ћелијску смрт индиректно, смањењем оксигенације
ткива, или директно, оштећењем мембране ћелија у ткивима. Животиње
угињавају услед масивних некроза и крвављења праћених запаљењем у ткивима
и органима. У зависности од проузроковача некротично-хеморагичне промене
и запаљења могу да настану најчешће у дигестивном систему, јетри, централном
нервном систему, срцу и скелетној мускулатури различитих животињских
врста. Сваки тип клостридијалних токсина утиче на појаву карактеристичних
морфолошких промена код појединих животињских врста., Clostridial infections in animals are caused by anaerobic bacteria of the genus
Clostridium. Clostridium species are widely distributed in environment and highly
resistant bacteria, due to their ability to form a spore and survive for a long time. They
represent residential microorganisms in the gastrointestinal tract of animals and
humans. In healthy animals, change in diet, injuries or weakened immune system most
commonly trigger the onset clostridial infections. There is a large number of different
species of genus Clostridium, but not all species of clostridia cause disease.
Pathogenic species of clostridia cause animal health disorders, most often with a fatal
outcome. The rate of mortality in clostridial infections is high. Due to this fact in the
farm breeding of animals, large economic losses can occur. The most important
pathogenic bacteria of the genus Clostridium are: Cl. perfrigens, Cl. chauvoei, Cl.
haemoliticum, Cl. septicum, Cl. novyi, Cl. pyliformis, Cl. tetani and Cl. botulinum.
Most commonly, infection is caused by single bacterial species, but sometimes it is
caused by variety of pathogenic clostridial species. The pathogenic effect of clostridia
occurs after the multiplication and release of different types of toxins, which can cause
local or systemic bleeding and necrotic changes. In some cases, gases accumulate in
the tissues and organs. The bacterial toxins can cause cell death indirectly, by reducing
tissue oxygenation, or directly by damaging plasma membrane of cells in tissues.
Animals could die due to massive necrosis and bleeding accompanied by
inflammation in tissues and organs. Depending on the causative factor, necrotichemorrhagic changes and inflammation can occur most often in the gastrointestinal
tract, liver, central nervous system, heart and skeletal muscle of many animal species.
Each type of clostridial toxins cause the characteristic morphological changes in
certain animal species.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019",
title = "Morfološke manifestacije klostridijalnih infekcija kod različitih životinjskih vrsta, Morphological changes due to clostridial infections in different animal species",
pages = "158-159",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3053"
}

Nešić, S., Marinković, D., Kukolj, V., Vučićević, I., Aničić, M.,& Aleksić-Kovačević, S.. (2019). Morfološke manifestacije klostridijalnih infekcija kod različitih životinjskih vrsta. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 158-159.
https://hdl.handle.net/21.15107/rcub_veterinar_3053

Nešić S, Marinković D, Kukolj V, Vučićević I, Aničić M, Aleksić-Kovačević S. Morfološke manifestacije klostridijalnih infekcija kod različitih životinjskih vrsta. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019. 2019;:158-159.
https://hdl.handle.net/21.15107/rcub_veterinar_3053 .

Nešić, Slađan, Marinković, Darko, Kukolj, Vladimir, Vučićević, Ivana, Aničić, Milan, Aleksić-Kovačević, Sanja, "Morfološke manifestacije klostridijalnih infekcija kod različitih životinjskih vrsta" in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019 (2019):158-159,
https://hdl.handle.net/21.15107/rcub_veterinar_3053 .

TY  - CONF
AU  - Vučićević, Ivana
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Nešić, Slađan
AU  - Aničić, Milan
AU  - Đurđević, Biljana
AU  - Aleksić-Kovačević, Sanja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3054
AB  - Циљ овог испитивања је рекласификација 17 узорака тумора паса који су
првобитном хистопатолошком анализом дијагностиковани као тумори овојница
периферних нерава. Главни критеријум за рекласификацију била је
имунохистохемијска позитивност на различите антигене.
Тумори овојница периферних нерава обухватају хетерогену групу неоплазми
које потичу од елемената који граде нервне овојнице. Ове неоплазме се могу
поделити у две групе: бенигне и малигне туморе овојница периферних нерава.
Бенигни тумори овојница периферних нерава нису заступљени у великом броју
код домацћих животиња, али је њихова појава забележена код различитих
животињских врста, као што су мачка, коњ, свиње и козе, при чему се најчешће
јавља код паса и говеда. Малигни тумори овојница периферних нерава се такође
релативно ретко дијагностикују код животиња и чине 27% свих тумора нервног
система. Хистолошки, бенигни тумори овојница периферних нерава су обично
добро ограничене, инкапсулисане новотворевине, претежно састављене од
вретенастих ћелија распоређених у снопове, палисадно или вртложно. Малигни
тумори овојница периферних нерава су неинкапсулисани и карактерише их
изражен полиморфизам ћелија распоређених у виду снопова или спирала са
бројним митотским фигурама.
Тумори овојница периферних нерава су често хистолошки врло слични другим
туморима насталим пролиферацијом ћелија вретенастог облика, те је стога
неопходно урадити имунохистохемијска бојења ради постављања
диференцијалне дијагнозе. Ове неоплазме обично показују имунохистохемијску позитивност на виментин, S-100, глијални фибриларни кисели
протеин, колаген типа IV и ламинин.
Четири тумора су била имунохистохемијски позитивна на мишићни маркер αSMA и виментин, а негативни на S-100 и дезмин. Присуство вретенастих ћелија
које стварају вртлоге око неколико крвних судова уочене су у овим туморима,
те су они рекласификовани у периваскуларне туморе. Осталих 13 тумора били
су S-100 и виментин позитивни, а α-SMA и дезмин негативни, чиме је потврђена
првобитна дијагноза да се ради о туморима овојница периферних нерава.
Разликовање тумора овојница периферних нерава и периваскуларних тумора
није једноставно ни поред примене имунохистохемијског панела.
AB  - The aim of this study was reclassification of 17 dog tumor samples initially diagnosed
with peripheral nerve sheath tumors (PNST) using histopathological analysis. The
main criteria for reclassification were immunochistohemical positivity on various
antigens.
Peripheral nerve sheath tumors comprise a heterogeneous group of neoplasms
originating from the elements of the nerve sheath. They are divided into two forms:
benign and malignant PNST. Benign PNSTs are not very common in domestic
animals but they are reported in different animal species, such as cat, horse, pig and
goat and most frequently are seen in dogs and cattle. Likewise, malignant PNSTs are
rare neoplasm in animals and they account for 27% of all nervous system tumors.
Histologically, benign PNSTs are usually well circumscribed, encapsulated and
composed predominantly of spindle cells arranged in bundles and sheets, while
malignant PNSTs are encapsulated and consist of highly pleomorphic cells with
numerous mitotic figures arranged in fascicles, whorls or sheets.
PNSTs are often histologically very similar to other spindle cell tumors and
immunohistochemistry is required for differential diagnosis. PNSTs generally express
vimentin, S-100, glial fibrillary acidic protein, collagen IV and laminin.
Four tumor samples were positive on muscular marker α-SMA and vimentin and
negative for S-100 and desmin. The spindle cells whirling around some blood vessels
were observed in these tumors so they are reclassified as perivascular tumors (PWTs).
The other 13 tumors were S-100 and vimentin positive and α-SMA and desmin
negative, thus classified as PNST.
Even after using the immunohistochemical panel, distinguishing between PNSTs and
PWTs is a quite difficult.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
T1  - Re-evaluacija tumora ovojnica perifernih nerava pasa
T1  - Reevaluation of canine peripheral nerve sheath tumors (pnst)
SP  - 176
EP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3054
ER  - 

@conference{
author = "Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Nešić, Slađan and Aničić, Milan and Đurđević, Biljana and Aleksić-Kovačević, Sanja",
year = "2019",
abstract = "Циљ овог испитивања је рекласификација 17 узорака тумора паса који су
првобитном хистопатолошком анализом дијагностиковани као тумори овојница
периферних нерава. Главни критеријум за рекласификацију била је
имунохистохемијска позитивност на различите антигене.
Тумори овојница периферних нерава обухватају хетерогену групу неоплазми
које потичу од елемената који граде нервне овојнице. Ове неоплазме се могу
поделити у две групе: бенигне и малигне туморе овојница периферних нерава.
Бенигни тумори овојница периферних нерава нису заступљени у великом броју
код домацћих животиња, али је њихова појава забележена код различитих
животињских врста, као што су мачка, коњ, свиње и козе, при чему се најчешће
јавља код паса и говеда. Малигни тумори овојница периферних нерава се такође
релативно ретко дијагностикују код животиња и чине 27% свих тумора нервног
система. Хистолошки, бенигни тумори овојница периферних нерава су обично
добро ограничене, инкапсулисане новотворевине, претежно састављене од
вретенастих ћелија распоређених у снопове, палисадно или вртложно. Малигни
тумори овојница периферних нерава су неинкапсулисани и карактерише их
изражен полиморфизам ћелија распоређених у виду снопова или спирала са
бројним митотским фигурама.
Тумори овојница периферних нерава су често хистолошки врло слични другим
туморима насталим пролиферацијом ћелија вретенастог облика, те је стога
неопходно урадити имунохистохемијска бојења ради постављања
диференцијалне дијагнозе. Ове неоплазме обично показују имунохистохемијску позитивност на виментин, S-100, глијални фибриларни кисели
протеин, колаген типа IV и ламинин.
Четири тумора су била имунохистохемијски позитивна на мишићни маркер αSMA и виментин, а негативни на S-100 и дезмин. Присуство вретенастих ћелија
које стварају вртлоге око неколико крвних судова уочене су у овим туморима,
те су они рекласификовани у периваскуларне туморе. Осталих 13 тумора били
су S-100 и виментин позитивни, а α-SMA и дезмин негативни, чиме је потврђена
првобитна дијагноза да се ради о туморима овојница периферних нерава.
Разликовање тумора овојница периферних нерава и периваскуларних тумора
није једноставно ни поред примене имунохистохемијског панела., The aim of this study was reclassification of 17 dog tumor samples initially diagnosed
with peripheral nerve sheath tumors (PNST) using histopathological analysis. The
main criteria for reclassification were immunochistohemical positivity on various
antigens.
Peripheral nerve sheath tumors comprise a heterogeneous group of neoplasms
originating from the elements of the nerve sheath. They are divided into two forms:
benign and malignant PNST. Benign PNSTs are not very common in domestic
animals but they are reported in different animal species, such as cat, horse, pig and
goat and most frequently are seen in dogs and cattle. Likewise, malignant PNSTs are
rare neoplasm in animals and they account for 27% of all nervous system tumors.
Histologically, benign PNSTs are usually well circumscribed, encapsulated and
composed predominantly of spindle cells arranged in bundles and sheets, while
malignant PNSTs are encapsulated and consist of highly pleomorphic cells with
numerous mitotic figures arranged in fascicles, whorls or sheets.
PNSTs are often histologically very similar to other spindle cell tumors and
immunohistochemistry is required for differential diagnosis. PNSTs generally express
vimentin, S-100, glial fibrillary acidic protein, collagen IV and laminin.
Four tumor samples were positive on muscular marker α-SMA and vimentin and
negative for S-100 and desmin. The spindle cells whirling around some blood vessels
were observed in these tumors so they are reclassified as perivascular tumors (PWTs).
The other 13 tumors were S-100 and vimentin positive and α-SMA and desmin
negative, thus classified as PNST.
Even after using the immunohistochemical panel, distinguishing between PNSTs and
PWTs is a quite difficult.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019",
title = "Re-evaluacija tumora ovojnica perifernih nerava pasa, Reevaluation of canine peripheral nerve sheath tumors (pnst)",
pages = "176-177",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3054"
}

Vučićević, I., Marinković, D., Kukolj, V., Nešić, S., Aničić, M., Đurđević, B.,& Aleksić-Kovačević, S.. (2019). Re-evaluacija tumora ovojnica perifernih nerava pasa. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 176-177.
https://hdl.handle.net/21.15107/rcub_veterinar_3054

Vučićević I, Marinković D, Kukolj V, Nešić S, Aničić M, Đurđević B, Aleksić-Kovačević S. Re-evaluacija tumora ovojnica perifernih nerava pasa. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019. 2019;:176-177.
https://hdl.handle.net/21.15107/rcub_veterinar_3054 .

Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Nešić, Slađan, Aničić, Milan, Đurđević, Biljana, Aleksić-Kovačević, Sanja, "Re-evaluacija tumora ovojnica perifernih nerava pasa" in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 - 10. april 2019 (2019):176-177,
https://hdl.handle.net/21.15107/rcub_veterinar_3054 .

TY  - JOUR
AU  - Drašković, Vladimir
AU  - Bošnjak-Neumuller, Jasna
AU  - Vasiljević, Marko
AU  - Petrujkić, Branko
AU  - Aleksić, Nevenka
AU  - Kukolj, Vladimir
AU  - Stanimirović, Zoran
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1634
PB  - Elsevier Science Bv, Amsterdam
T2  - Preventive Veterinary Medicine
T1  - Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs (vol 151, pg 46, 2018)
VL  - 154
SP  - 29
EP  - 29
DO  - 10.1016/j.prevetmed.2018.03.007
ER  - 

@article{
author = "Drašković, Vladimir and Bošnjak-Neumuller, Jasna and Vasiljević, Marko and Petrujkić, Branko and Aleksić, Nevenka and Kukolj, Vladimir and Stanimirović, Zoran",
year = "2018",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Preventive Veterinary Medicine",
title = "Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs (vol 151, pg 46, 2018)",
volume = "154",
pages = "29-29",
doi = "10.1016/j.prevetmed.2018.03.007"
}

Drašković, V., Bošnjak-Neumuller, J., Vasiljević, M., Petrujkić, B., Aleksić, N., Kukolj, V.,& Stanimirović, Z.. (2018). Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs (vol 151, pg 46, 2018). in Preventive Veterinary Medicine
Elsevier Science Bv, Amsterdam., 154, 29-29.
https://doi.org/10.1016/j.prevetmed.2018.03.007

Drašković V, Bošnjak-Neumuller J, Vasiljević M, Petrujkić B, Aleksić N, Kukolj V, Stanimirović Z. Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs (vol 151, pg 46, 2018). in Preventive Veterinary Medicine. 2018;154:29-29.
doi:10.1016/j.prevetmed.2018.03.007 .

Drašković, Vladimir, Bošnjak-Neumuller, Jasna, Vasiljević, Marko, Petrujkić, Branko, Aleksić, Nevenka, Kukolj, Vladimir, Stanimirović, Zoran, "Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs (vol 151, pg 46, 2018)" in Preventive Veterinary Medicine, 154 (2018):29-29,
https://doi.org/10.1016/j.prevetmed.2018.03.007 . .

TY  - JOUR
AU  - Drašković, Vladimir
AU  - Bošnjak-Neumuller, Jasna
AU  - Vasiljević, Marko
AU  - Petrujkić, Branko
AU  - Aleksić, Nevenka
AU  - Kukolj, Vladimir
AU  - Stanimirović, Zoran
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1636
AB  - Lawsonia intracellularis is known to cause proliferative enteropathy (PE), one of the economically most important swine diseases with global distribution. Not unlike other enteric diseases, PE is a frequent indication for antibiotic therapy. However, their unjustified use leads to an emerging problem antimicrobial resistance. Thus, the aim of this research was to assess if a phytogenic additive may replace antibiotics in the control of PE in 144 weaned piglets (72 treated and 72 controls) naturally infected with L. intracellularis. The quantity of L intracellularis faecal shedding was monitored by real-time polymerase chain reaction (PCR) assay in faecal samples on day 0, 14 and 28, whilst the level of the ileum damage was determined by immunohistochemistry (IHC) assay performed on gut sections. Real-time PCR assay revealed that cycle-threshold (Ct) values in the treatment group increased significantly over time and were higher than in the control. These results indicate that the use of the phytogenic additive decreases the faecal excretion of L. intracellularis both throughout the experiment and in comparison to the control. The expression of the L. intracellularis antigen in IHC assay was lower in treated animals, implying that the additive leads to the decrease in the pathogen quantity in the ileum. Significantly higher feed conversion ratio was recorded in the treatment group. The results indicate that the phytogenic additive may be beneficial in the control of PE, but additional research is necessary to assess its use in various pig categories and define the optimum concentrations.
PB  - Elsevier Science Bv, Amsterdam
T2  - Preventive Veterinary Medicine
T1  - Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs
VL  - 151
SP  - 46
EP  - 51
DO  - 10.1016/j.prevetmed.2018.01.002
ER  - 

@article{
author = "Drašković, Vladimir and Bošnjak-Neumuller, Jasna and Vasiljević, Marko and Petrujkić, Branko and Aleksić, Nevenka and Kukolj, Vladimir and Stanimirović, Zoran",
year = "2018",
abstract = "Lawsonia intracellularis is known to cause proliferative enteropathy (PE), one of the economically most important swine diseases with global distribution. Not unlike other enteric diseases, PE is a frequent indication for antibiotic therapy. However, their unjustified use leads to an emerging problem antimicrobial resistance. Thus, the aim of this research was to assess if a phytogenic additive may replace antibiotics in the control of PE in 144 weaned piglets (72 treated and 72 controls) naturally infected with L. intracellularis. The quantity of L intracellularis faecal shedding was monitored by real-time polymerase chain reaction (PCR) assay in faecal samples on day 0, 14 and 28, whilst the level of the ileum damage was determined by immunohistochemistry (IHC) assay performed on gut sections. Real-time PCR assay revealed that cycle-threshold (Ct) values in the treatment group increased significantly over time and were higher than in the control. These results indicate that the use of the phytogenic additive decreases the faecal excretion of L. intracellularis both throughout the experiment and in comparison to the control. The expression of the L. intracellularis antigen in IHC assay was lower in treated animals, implying that the additive leads to the decrease in the pathogen quantity in the ileum. Significantly higher feed conversion ratio was recorded in the treatment group. The results indicate that the phytogenic additive may be beneficial in the control of PE, but additional research is necessary to assess its use in various pig categories and define the optimum concentrations.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Preventive Veterinary Medicine",
title = "Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs",
volume = "151",
pages = "46-51",
doi = "10.1016/j.prevetmed.2018.01.002"
}

Drašković, V., Bošnjak-Neumuller, J., Vasiljević, M., Petrujkić, B., Aleksić, N., Kukolj, V.,& Stanimirović, Z.. (2018). Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs. in Preventive Veterinary Medicine
Elsevier Science Bv, Amsterdam., 151, 46-51.
https://doi.org/10.1016/j.prevetmed.2018.01.002

Drašković V, Bošnjak-Neumuller J, Vasiljević M, Petrujkić B, Aleksić N, Kukolj V, Stanimirović Z. Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs. in Preventive Veterinary Medicine. 2018;151:46-51.
doi:10.1016/j.prevetmed.2018.01.002 .

Drašković, Vladimir, Bošnjak-Neumuller, Jasna, Vasiljević, Marko, Petrujkić, Branko, Aleksić, Nevenka, Kukolj, Vladimir, Stanimirović, Zoran, "Influence of phytogenic feed additive on Lawsonia intracellularis infection in pigs" in Preventive Veterinary Medicine, 151 (2018):46-51,
https://doi.org/10.1016/j.prevetmed.2018.01.002 . .

TY  - JOUR
AU  - Vučićević, Ivana
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Nešić, Slađan
AU  - Aničić, Milan
AU  - Aleksić-Kovačević, Sanja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1605
AB  - Uprkos činjenici da su mastocitomi jedna od najčešćih neoplazmi kod pasa, postoji samo jedan objavljeni slučaj mastocitoma u lipomu. U ovom slučaju, kod 11-godišnje ženke toj pudle primećeno je prisustvo dobro ograničene, pokretne, meko-elastične mase na lateralnoj strani grudnog koša. Histopatološki pregled je pokazao dobro ograničenu masu koja se sastojala od zrelih adipocita između kojih se nalaze skupine ćelija sa granuliranom citoplazmom. Bojenjem toluidin plavim je dokazano prisustvo metahromatskih granula u citoplazmi mastocita. Histopatološke i imunohistohemijske karakteristike ukazale su na to da je ispitani mastocitom niskog stepena maligniteta.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Mast cell tumor within a lipoma in a white toy poodle
T1  - Mastocitom unutar lipoma kod bele toj pudle
VL  - 72
IS  - 1
SP  - 64
EP  - 67
DO  - 10.2298/VETGL170611002V
ER  - 

@article{
author = "Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Nešić, Slađan and Aničić, Milan and Aleksić-Kovačević, Sanja",
year = "2018",
abstract = "Uprkos činjenici da su mastocitomi jedna od najčešćih neoplazmi kod pasa, postoji samo jedan objavljeni slučaj mastocitoma u lipomu. U ovom slučaju, kod 11-godišnje ženke toj pudle primećeno je prisustvo dobro ograničene, pokretne, meko-elastične mase na lateralnoj strani grudnog koša. Histopatološki pregled je pokazao dobro ograničenu masu koja se sastojala od zrelih adipocita između kojih se nalaze skupine ćelija sa granuliranom citoplazmom. Bojenjem toluidin plavim je dokazano prisustvo metahromatskih granula u citoplazmi mastocita. Histopatološke i imunohistohemijske karakteristike ukazale su na to da je ispitani mastocitom niskog stepena maligniteta.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Mast cell tumor within a lipoma in a white toy poodle, Mastocitom unutar lipoma kod bele toj pudle",
volume = "72",
number = "1",
pages = "64-67",
doi = "10.2298/VETGL170611002V"
}

Vučićević, I., Marinković, D., Kukolj, V., Nešić, S., Aničić, M.,& Aleksić-Kovačević, S.. (2018). Mast cell tumor within a lipoma in a white toy poodle. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 72(1), 64-67.
https://doi.org/10.2298/VETGL170611002V

Vučićević I, Marinković D, Kukolj V, Nešić S, Aničić M, Aleksić-Kovačević S. Mast cell tumor within a lipoma in a white toy poodle. in Veterinarski Glasnik. 2018;72(1):64-67.
doi:10.2298/VETGL170611002V .

Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Nešić, Slađan, Aničić, Milan, Aleksić-Kovačević, Sanja, "Mast cell tumor within a lipoma in a white toy poodle" in Veterinarski Glasnik, 72, no. 1 (2018):64-67,
https://doi.org/10.2298/VETGL170611002V . .

TY  - JOUR
AU  - Vučićević, Ivana
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Nešić, Slađan
AU  - Aničić, Milan
AU  - Aleksić-Kovačević, Sanja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2174
AB  - Mastocitomi su najučestaliji tumori kože pasa. Vode poreklo iz
kostne srži, a zbog vrlo promenljivog biološkog ponašanja je predloženo nekoliko
kriterijuma za njihovu klasifikaciju, uključujući histološku gradaciju i markere
proliferacije ćelija. Mitotski indeks, prisustvo višejedarnih ćelija, ćelija sa bizarnim
jedrom i kariomegalija su najvažniji parametri za klasifikaciju mastocitoma pasa na
mastocitome visokog i niskog stepena maligniteta. Za potvrdu stepena maligniteta
mastocitoma bitno je utvrditi i ekspresiju transmembranskog receptora kinaza tipa
(KIT) koji igra značajnu ulogu u rastu i diferencijaciji mastocita, a koga kodira c-KIT
protoonkogen. Kod mastocitoma visokog stepena maligniteta, osim membranske
ekspresije KIT receptora, može se uočiti i aberantna citoplazmatska ekspresija.
Ispitana su ukupno 52 mastocitoma pasa i 12 uzoraka nepromenjene kože pasa.
Uzorci kože fiksirani u formalinu i ukalupljeni u parafinu su obojeni hematoksilineozinom.
Ekspresija KIT receptora je ispitana primenom imunohistohemijske
metode bojenja. Klasifikacija mastocitoma na mastocitome visokog i niskog stepena
maligniteta je izvršena prema kriterijumima Kiupel-ove dvostepene klasifikacije,
uzimajući u obzir i ekspresiju KIT receptora. Od ukupno 52 mastocitoma pasa 16
je bilo niskog stepena maligniteta, dok je 36 klasifikovano kao mastocitomi visokog
stepena maligniteta, od kojih 19 mastocitoma pokazuje citoplazmatsku ekspresiju
KIT receptora. Kod svih 16 mastocitoma niskog stepena maligniteta ispoljena je
samo membranska ekspresija KIT proteina. Činjenica da je kod većine mastocitoma
visokog stepena maligniteta ispoljena ekspresija KIT receptora u citoplazmi potvrđuje
vezu između aberantne ekspresije KIT receptora i povećane ćelijske proliferacije.
Prisustvo mitotskih figura, višejedarnih ćelija, bizarnih jedara i kariomegalije, kao i tip ekspresije KIT receptora predstavljaju ključne prognostičke parametre kod pasa
sa mastocitomima.
AB  - Mast cell tumors (MCTs) are one of the most common neoplasms in
dogs. They originate from the bone marrow. Several criteria have been proposed
for their classification, including histological grading and cell proliferation markers.
The mitotic index, multiple nuclei, cells with bizarre nuclei and karyomegaly are the
most important parameters for the classification of MCTs on high and low-grade
malignancy. For the confirmation of MCT malignancy grade it is also important to
determine the expression of the transmembrane kinase (KIT) receptor that has a
significant role in the growth and differentiation of mast cell tumors. In high-grade
malignancy MCTs in addition to membrane expression of the KIT receptor, aberrant
cytoplasmic expression can be also observed. Fifty-two MCTs and 12 control
samples of skin from dogs without MCTs were examined. Routinely processed tissue
samples were stained with hematoxylin-eosin. Expression of KIT receptor was
tested immunohistochemically. The classification of MCTs on high and low-grade
malignancy was performed according to the Kiupel’s 2-tier classification and the
expression of KIT receptors. Sixteen of 52 MCTs were of a low-grade malignancy,
while thirty-six were classified as a high-grade malignancy of which 19 MCTs
showed aberrant cytoplasm labelling of KIT receptor. All 16 low-grade malignancy
MCTs had only membrane expression of KIT receptor. Most high-grade malignancy
MCTs showed cytoplasmic KIT expression indicating the link between aberrant
KIT expression and increased cell proliferation. The presence of mitotic figures,
multinucleated cells, bizarre nuclei and karyomegaly, as well as KIT receptor expression pattern are the most important prognostic factors in dogs with mast cell
tumor.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Histološki i imunohistohemijski parametri bitni za klasifikaciju mastocitoma pasa
T1  - Important histological and immunochemical parameters for classification of canine mast cell tumors
VL  - 18
IS  - 2
SP  - 370
EP  - 391
DO  - 10.7251/VETJ1802370V
ER  - 

@article{
author = "Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Nešić, Slađan and Aničić, Milan and Aleksić-Kovačević, Sanja",
year = "2018",
abstract = "Mastocitomi su najučestaliji tumori kože pasa. Vode poreklo iz
kostne srži, a zbog vrlo promenljivog biološkog ponašanja je predloženo nekoliko
kriterijuma za njihovu klasifikaciju, uključujući histološku gradaciju i markere
proliferacije ćelija. Mitotski indeks, prisustvo višejedarnih ćelija, ćelija sa bizarnim
jedrom i kariomegalija su najvažniji parametri za klasifikaciju mastocitoma pasa na
mastocitome visokog i niskog stepena maligniteta. Za potvrdu stepena maligniteta
mastocitoma bitno je utvrditi i ekspresiju transmembranskog receptora kinaza tipa
(KIT) koji igra značajnu ulogu u rastu i diferencijaciji mastocita, a koga kodira c-KIT
protoonkogen. Kod mastocitoma visokog stepena maligniteta, osim membranske
ekspresije KIT receptora, može se uočiti i aberantna citoplazmatska ekspresija.
Ispitana su ukupno 52 mastocitoma pasa i 12 uzoraka nepromenjene kože pasa.
Uzorci kože fiksirani u formalinu i ukalupljeni u parafinu su obojeni hematoksilineozinom.
Ekspresija KIT receptora je ispitana primenom imunohistohemijske
metode bojenja. Klasifikacija mastocitoma na mastocitome visokog i niskog stepena
maligniteta je izvršena prema kriterijumima Kiupel-ove dvostepene klasifikacije,
uzimajući u obzir i ekspresiju KIT receptora. Od ukupno 52 mastocitoma pasa 16
je bilo niskog stepena maligniteta, dok je 36 klasifikovano kao mastocitomi visokog
stepena maligniteta, od kojih 19 mastocitoma pokazuje citoplazmatsku ekspresiju
KIT receptora. Kod svih 16 mastocitoma niskog stepena maligniteta ispoljena je
samo membranska ekspresija KIT proteina. Činjenica da je kod većine mastocitoma
visokog stepena maligniteta ispoljena ekspresija KIT receptora u citoplazmi potvrđuje
vezu između aberantne ekspresije KIT receptora i povećane ćelijske proliferacije.
Prisustvo mitotskih figura, višejedarnih ćelija, bizarnih jedara i kariomegalije, kao i tip ekspresije KIT receptora predstavljaju ključne prognostičke parametre kod pasa
sa mastocitomima., Mast cell tumors (MCTs) are one of the most common neoplasms in
dogs. They originate from the bone marrow. Several criteria have been proposed
for their classification, including histological grading and cell proliferation markers.
The mitotic index, multiple nuclei, cells with bizarre nuclei and karyomegaly are the
most important parameters for the classification of MCTs on high and low-grade
malignancy. For the confirmation of MCT malignancy grade it is also important to
determine the expression of the transmembrane kinase (KIT) receptor that has a
significant role in the growth and differentiation of mast cell tumors. In high-grade
malignancy MCTs in addition to membrane expression of the KIT receptor, aberrant
cytoplasmic expression can be also observed. Fifty-two MCTs and 12 control
samples of skin from dogs without MCTs were examined. Routinely processed tissue
samples were stained with hematoxylin-eosin. Expression of KIT receptor was
tested immunohistochemically. The classification of MCTs on high and low-grade
malignancy was performed according to the Kiupel’s 2-tier classification and the
expression of KIT receptors. Sixteen of 52 MCTs were of a low-grade malignancy,
while thirty-six were classified as a high-grade malignancy of which 19 MCTs
showed aberrant cytoplasm labelling of KIT receptor. All 16 low-grade malignancy
MCTs had only membrane expression of KIT receptor. Most high-grade malignancy
MCTs showed cytoplasmic KIT expression indicating the link between aberrant
KIT expression and increased cell proliferation. The presence of mitotic figures,
multinucleated cells, bizarre nuclei and karyomegaly, as well as KIT receptor expression pattern are the most important prognostic factors in dogs with mast cell
tumor.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Histološki i imunohistohemijski parametri bitni za klasifikaciju mastocitoma pasa, Important histological and immunochemical parameters for classification of canine mast cell tumors",
volume = "18",
number = "2",
pages = "370-391",
doi = "10.7251/VETJ1802370V"
}

Vučićević, I., Marinković, D., Kukolj, V., Nešić, S., Aničić, M.,& Aleksić-Kovačević, S.. (2018). Histološki i imunohistohemijski parametri bitni za klasifikaciju mastocitoma pasa. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 18(2), 370-391.
https://doi.org/10.7251/VETJ1802370V

Vučićević I, Marinković D, Kukolj V, Nešić S, Aničić M, Aleksić-Kovačević S. Histološki i imunohistohemijski parametri bitni za klasifikaciju mastocitoma pasa. in Veterinarski žurnal Republike Srpske. 2018;18(2):370-391.
doi:10.7251/VETJ1802370V .

Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Nešić, Slađan, Aničić, Milan, Aleksić-Kovačević, Sanja, "Histološki i imunohistohemijski parametri bitni za klasifikaciju mastocitoma pasa" in Veterinarski žurnal Republike Srpske, 18, no. 2 (2018):370-391,
https://doi.org/10.7251/VETJ1802370V . .

TY  - CONF
AU  - Aničić, Milan
AU  - Marinković, Darko
AU  - Vasković, Nikola
AU  - Vučićević, Ivana
AU  - Nešić, Slađan
AU  - Kukolj, Vladimir
AU  - Aleksić-Kovačević, Sanja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3050
AB  - Штенећак представља заразно, често фатално обољење распрострањено широм
света изазвано морбиливирусом из фамилије Paramyxoviridae. Природни
домаћини су животиње из реда Carnivora. Постоји један серотип вируса са
неколико генотипова различите вируленције и ћелијског тропизма. Болест
протиче у акутној, катаралној форми (компликована секундарним бактеријским
инфекцијама услед изражене имуносупресије) и/или хроничној, нервној форми.
Неуобичајене клиничке манифестације обухватају енцефалитис старих паса и
болест тврдих шапа. У зависности од генотипа вируса нервна форма се
карактерише појавом полиоенцефалитиса или леукоенцефаломијелитиса са
демијелинизацијом. Цитоплазматске и интрануклеарне инклузије се могу
пронаћи у многим органима.
У овом раду су описане патоморфолошке промене на мозговима лисица
серолошки позитивних на вирус штенећака. Код већине животиња су уочени
знаци дегенерације неурона, глиоза и сателитоза различитог степена.
Еозинофилне, округле до овалне инклузије у неуронима и/или глија ћелијама су
биле присутне код више од половине прегледаних мозгова. Код скоро половине
животиња је уочена инфламаторни реакција, првенствено састављена од благог
до умереног периваскуларног мононуклеарног инфилтрата. Код исто толико
животиња су присутни знаци демијелинизације, највише изражени у белој маси
малог мозга. Местимично се уочавају ситна крвављења, дегенерација
епендимских ћелија, едем неуропила и умножавање ситних крвних судова. По
једна животиња показивала је знаке калцификације као и синцицијум
епендимских ћелија.
Разлике у патоморфолошким променама могу бити последица инфекције
различитим генотиповима вируса што ће показати имунохистохемијска и
молекуларна испитивања. Континуирано откривање нових врста пријемчивих
на инфекцију вирусом штенећака потенцира наставак праћења и изучавања овог
узрочника. Посебан акценат се ставља на могућност инфекције људи, посебно
оних који немају развијен имунитет према узрочнику малих богиња.
AB  - Distemper is a contagious, often fatal, worldwide distributed disease caused by
Morbillivirus of family Paramixoviridae. Natural hosts comprise animals of order
Carnivora. There is one virus serotype with several genotypes of different virulence
and cell tropism. Disease manifests as acute, catarrhal (often complicated with
secondary bacterial infections due to severe immunosuppression) and/or chronic,
nervous form. Old dog encephalitis and hard pad disease are unusual clinical
manifestations. Depending on viral genotype, nervous form manifests as
polioencephalitis or demyelinating leukoencephalomyelitis. Citoplasmatic and
intranuclear inclusion bodies can be found in many organs.
This work describes pathomorphological changes in the brains of serologically CDVpositive foxes. Many animals had neuronal degeneration, gliosis and satelitosis of
various degree. Eosinophilic, round to oval inclusion bodies in neurons and/or glial
cells were seen in more than half examined brains. Almost half of them had
inflammatory reaction, consisted mainly of mild to moderate perivascular
mononuclear infiltrate, as well as signs of demyelination, mostly in the cerebellar
white matter. Occasionally small haemorrhages, ependimal cell degeneration, oedema
of neuropil and proliferation of small blood vessels were seen. In one animal there
was calcification and also one animal showed signs of ependimal cell syncytium
development.
Differences in pathomorphological changes could be a consequence of an infection
with different viral genotype which will be confirmed with immunohistochemical and
molecular examinations. Constant growth of CDV natural host spectrum demands
thorough studies and close follow up of this infectious agent. Of special concern is
possible human infection, primarily those who do not have immunity against measles.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XX Simpozijum epizootiologa i epidemiologa (XX Epizootiološki dani), Vrnjačka banja, 18 - 20. april 2018
T1  - Patomorfološke promene na mozgovima lisica serološki pozitivnih na štenećak
T1  - Pathomorphological changes in the brains of serologicaly cdv-positive foxes
SP  - 114
EP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3050
ER  - 

@conference{
author = "Aničić, Milan and Marinković, Darko and Vasković, Nikola and Vučićević, Ivana and Nešić, Slađan and Kukolj, Vladimir and Aleksić-Kovačević, Sanja",
year = "2018",
abstract = "Штенећак представља заразно, често фатално обољење распрострањено широм
света изазвано морбиливирусом из фамилије Paramyxoviridae. Природни
домаћини су животиње из реда Carnivora. Постоји један серотип вируса са
неколико генотипова различите вируленције и ћелијског тропизма. Болест
протиче у акутној, катаралној форми (компликована секундарним бактеријским
инфекцијама услед изражене имуносупресије) и/или хроничној, нервној форми.
Неуобичајене клиничке манифестације обухватају енцефалитис старих паса и
болест тврдих шапа. У зависности од генотипа вируса нервна форма се
карактерише појавом полиоенцефалитиса или леукоенцефаломијелитиса са
демијелинизацијом. Цитоплазматске и интрануклеарне инклузије се могу
пронаћи у многим органима.
У овом раду су описане патоморфолошке промене на мозговима лисица
серолошки позитивних на вирус штенећака. Код већине животиња су уочени
знаци дегенерације неурона, глиоза и сателитоза различитог степена.
Еозинофилне, округле до овалне инклузије у неуронима и/или глија ћелијама су
биле присутне код више од половине прегледаних мозгова. Код скоро половине
животиња је уочена инфламаторни реакција, првенствено састављена од благог
до умереног периваскуларног мононуклеарног инфилтрата. Код исто толико
животиња су присутни знаци демијелинизације, највише изражени у белој маси
малог мозга. Местимично се уочавају ситна крвављења, дегенерација
епендимских ћелија, едем неуропила и умножавање ситних крвних судова. По
једна животиња показивала је знаке калцификације као и синцицијум
епендимских ћелија.
Разлике у патоморфолошким променама могу бити последица инфекције
различитим генотиповима вируса што ће показати имунохистохемијска и
молекуларна испитивања. Континуирано откривање нових врста пријемчивих
на инфекцију вирусом штенећака потенцира наставак праћења и изучавања овог
узрочника. Посебан акценат се ставља на могућност инфекције људи, посебно
оних који немају развијен имунитет према узрочнику малих богиња., Distemper is a contagious, often fatal, worldwide distributed disease caused by
Morbillivirus of family Paramixoviridae. Natural hosts comprise animals of order
Carnivora. There is one virus serotype with several genotypes of different virulence
and cell tropism. Disease manifests as acute, catarrhal (often complicated with
secondary bacterial infections due to severe immunosuppression) and/or chronic,
nervous form. Old dog encephalitis and hard pad disease are unusual clinical
manifestations. Depending on viral genotype, nervous form manifests as
polioencephalitis or demyelinating leukoencephalomyelitis. Citoplasmatic and
intranuclear inclusion bodies can be found in many organs.
This work describes pathomorphological changes in the brains of serologically CDVpositive foxes. Many animals had neuronal degeneration, gliosis and satelitosis of
various degree. Eosinophilic, round to oval inclusion bodies in neurons and/or glial
cells were seen in more than half examined brains. Almost half of them had
inflammatory reaction, consisted mainly of mild to moderate perivascular
mononuclear infiltrate, as well as signs of demyelination, mostly in the cerebellar
white matter. Occasionally small haemorrhages, ependimal cell degeneration, oedema
of neuropil and proliferation of small blood vessels were seen. In one animal there
was calcification and also one animal showed signs of ependimal cell syncytium
development.
Differences in pathomorphological changes could be a consequence of an infection
with different viral genotype which will be confirmed with immunohistochemical and
molecular examinations. Constant growth of CDV natural host spectrum demands
thorough studies and close follow up of this infectious agent. Of special concern is
possible human infection, primarily those who do not have immunity against measles.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XX Simpozijum epizootiologa i epidemiologa (XX Epizootiološki dani), Vrnjačka banja, 18 - 20. april 2018",
title = "Patomorfološke promene na mozgovima lisica serološki pozitivnih na štenećak, Pathomorphological changes in the brains of serologicaly cdv-positive foxes",
pages = "114-115",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3050"
}

Aničić, M., Marinković, D., Vasković, N., Vučićević, I., Nešić, S., Kukolj, V.,& Aleksić-Kovačević, S.. (2018). Patomorfološke promene na mozgovima lisica serološki pozitivnih na štenećak. in XX Simpozijum epizootiologa i epidemiologa (XX Epizootiološki dani), Vrnjačka banja, 18 - 20. april 2018
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 114-115.
https://hdl.handle.net/21.15107/rcub_veterinar_3050

Aničić M, Marinković D, Vasković N, Vučićević I, Nešić S, Kukolj V, Aleksić-Kovačević S. Patomorfološke promene na mozgovima lisica serološki pozitivnih na štenećak. in XX Simpozijum epizootiologa i epidemiologa (XX Epizootiološki dani), Vrnjačka banja, 18 - 20. april 2018. 2018;:114-115.
https://hdl.handle.net/21.15107/rcub_veterinar_3050 .

Aničić, Milan, Marinković, Darko, Vasković, Nikola, Vučićević, Ivana, Nešić, Slađan, Kukolj, Vladimir, Aleksić-Kovačević, Sanja, "Patomorfološke promene na mozgovima lisica serološki pozitivnih na štenećak" in XX Simpozijum epizootiologa i epidemiologa (XX Epizootiološki dani), Vrnjačka banja, 18 - 20. april 2018 (2018):114-115,
https://hdl.handle.net/21.15107/rcub_veterinar_3050 .

TY  - JOUR
AU  - Nešić, Slađan
AU  - Kukolj, Vladimir
AU  - Marinković, Darko
AU  - Vučićević, Ivana
AU  - Jovanović, Milijan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1508
AB  - Background: Cerebral amyloid angiopathy (CAA) is a disorder characterized by amyloid deposition in the wall of cerebral blood vessels. The deposits of amyloid occur frequently in the blood vessels of the frontal, parietal and occipital cortex. Objective: To examine the characteristics of CAA classified according to the Vonsattel scale in elderly dogs histologically and immunohistochemically as well as the semi-quantitative evaluation of the amyloid deposits in the different segments of the brain. Animals and methods: The brains of 36 dogs of different breeds and sexes, which had been routinely necropsied, were used and divided into two groups: dogs from 1 to 5 and 10 to 18 years old. The tissue sections were stained by hematoxylin-eosin, Congo red and immunohistochemically. Results: Amyloid was accumulated in the wall of cerebral blood vessels in 70% of dogs over the age of 10 years predominantly in the frontal cortex. CAA was demonstrated in elderly dogs as follows: in the frontal cortex (n=19 or 63%), the parietal cortex (n=12 or 40%), the hippocampus (40%) and the cerebellum (n=5 or 17%). The deposits of amyloid in the wall of blood vessels detected by Congo red staining were also A beta 1-14 and A beta 1-42 immunohistochemically positive. Most commonly, the amyloid deposits affected a moderate number of blood vessels. The accumulation of amyloid was immunohistochemically revealed in the blood vessel walls as well as in the senile plaques and neurons. Conclusion: The amount of amyloid in the arterial walls increased with age in dogs, whereas the amyloid accumulated in plaques was Congo red negative.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Veterinary Quarterly
T1  - Histological and immunohistochemical characteristics of cerebral amyloid angiopathy in elderly dogs
VL  - 37
IS  - 1
SP  - 1
EP  - 7
DO  - 10.1080/01652176.2016.1235301
ER  - 

@article{
author = "Nešić, Slađan and Kukolj, Vladimir and Marinković, Darko and Vučićević, Ivana and Jovanović, Milijan",
year = "2017",
abstract = "Background: Cerebral amyloid angiopathy (CAA) is a disorder characterized by amyloid deposition in the wall of cerebral blood vessels. The deposits of amyloid occur frequently in the blood vessels of the frontal, parietal and occipital cortex. Objective: To examine the characteristics of CAA classified according to the Vonsattel scale in elderly dogs histologically and immunohistochemically as well as the semi-quantitative evaluation of the amyloid deposits in the different segments of the brain. Animals and methods: The brains of 36 dogs of different breeds and sexes, which had been routinely necropsied, were used and divided into two groups: dogs from 1 to 5 and 10 to 18 years old. The tissue sections were stained by hematoxylin-eosin, Congo red and immunohistochemically. Results: Amyloid was accumulated in the wall of cerebral blood vessels in 70% of dogs over the age of 10 years predominantly in the frontal cortex. CAA was demonstrated in elderly dogs as follows: in the frontal cortex (n=19 or 63%), the parietal cortex (n=12 or 40%), the hippocampus (40%) and the cerebellum (n=5 or 17%). The deposits of amyloid in the wall of blood vessels detected by Congo red staining were also A beta 1-14 and A beta 1-42 immunohistochemically positive. Most commonly, the amyloid deposits affected a moderate number of blood vessels. The accumulation of amyloid was immunohistochemically revealed in the blood vessel walls as well as in the senile plaques and neurons. Conclusion: The amount of amyloid in the arterial walls increased with age in dogs, whereas the amyloid accumulated in plaques was Congo red negative.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Veterinary Quarterly",
title = "Histological and immunohistochemical characteristics of cerebral amyloid angiopathy in elderly dogs",
volume = "37",
number = "1",
pages = "1-7",
doi = "10.1080/01652176.2016.1235301"
}

Nešić, S., Kukolj, V., Marinković, D., Vučićević, I.,& Jovanović, M.. (2017). Histological and immunohistochemical characteristics of cerebral amyloid angiopathy in elderly dogs. in Veterinary Quarterly
Taylor & Francis Ltd, Abingdon., 37(1), 1-7.
https://doi.org/10.1080/01652176.2016.1235301

Nešić S, Kukolj V, Marinković D, Vučićević I, Jovanović M. Histological and immunohistochemical characteristics of cerebral amyloid angiopathy in elderly dogs. in Veterinary Quarterly. 2017;37(1):1-7.
doi:10.1080/01652176.2016.1235301 .

Nešić, Slađan, Kukolj, Vladimir, Marinković, Darko, Vučićević, Ivana, Jovanović, Milijan, "Histological and immunohistochemical characteristics of cerebral amyloid angiopathy in elderly dogs" in Veterinary Quarterly, 37, no. 1 (2017):1-7,
https://doi.org/10.1080/01652176.2016.1235301 . .

TY  - JOUR
AU  - Aničić, Milan
AU  - Kukolj, Vladimir
AU  - Marinković, Darko
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1538
AB  - Equine glanders is a severe bacterial disease known since ancient times. Although eradicated in the most part of the world it is now considered re-emerging. Considering very scarce literature data, we used from formalin fixed collection material: nasal septum, lung and skin specimens from naturally infected horses. Tissues were grossly examined and photographed. Tissue samples, after standard processing, were stained with HE, Congo red and Groccot and microscopically examined. Gross changes include nodules and ulcers in the nasal mucosa with granulation and scarring, pyogranulomas in the lung tissue and nodules and ulcers of the skin. Microscopically marked inflammation of affected tissues with neutrophilic and mononuclear infiltration and fibrous tissue proliferation were seen. As a potent zoonotic agent it has been already used as a biological weapon in the past.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Morphological characteristics of three classic forms of natural equine glanders - a disease with high zoonotic significance
VL  - 67
IS  - 4
SP  - 572
EP  - 577
DO  - 10.1515/acve-2017-0045
ER  - 

@article{
author = "Aničić, Milan and Kukolj, Vladimir and Marinković, Darko",
year = "2017",
abstract = "Equine glanders is a severe bacterial disease known since ancient times. Although eradicated in the most part of the world it is now considered re-emerging. Considering very scarce literature data, we used from formalin fixed collection material: nasal septum, lung and skin specimens from naturally infected horses. Tissues were grossly examined and photographed. Tissue samples, after standard processing, were stained with HE, Congo red and Groccot and microscopically examined. Gross changes include nodules and ulcers in the nasal mucosa with granulation and scarring, pyogranulomas in the lung tissue and nodules and ulcers of the skin. Microscopically marked inflammation of affected tissues with neutrophilic and mononuclear infiltration and fibrous tissue proliferation were seen. As a potent zoonotic agent it has been already used as a biological weapon in the past.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Morphological characteristics of three classic forms of natural equine glanders - a disease with high zoonotic significance",
volume = "67",
number = "4",
pages = "572-577",
doi = "10.1515/acve-2017-0045"
}

Aničić, M., Kukolj, V.,& Marinković, D.. (2017). Morphological characteristics of three classic forms of natural equine glanders - a disease with high zoonotic significance. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 572-577.
https://doi.org/10.1515/acve-2017-0045

Aničić M, Kukolj V, Marinković D. Morphological characteristics of three classic forms of natural equine glanders - a disease with high zoonotic significance. in Acta Veterinaria-Beograd. 2017;67(4):572-577.
doi:10.1515/acve-2017-0045 .

Aničić, Milan, Kukolj, Vladimir, Marinković, Darko, "Morphological characteristics of three classic forms of natural equine glanders - a disease with high zoonotic significance" in Acta Veterinaria-Beograd, 67, no. 4 (2017):572-577,
https://doi.org/10.1515/acve-2017-0045 . .

TY  - CONF
AU  - Aleksić-Kovačević, Sanja
AU  - Kukolj, Vladimir
AU  - Nešić, Slađan
AU  - Vučićević, Ivana
AU  - Aničić, Milan
AU  - Marinković, Darko
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2957
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 22. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 7-10.jun 2017
T1  - Koliko nam je neophodan veterinarski patolog?
T1  - Do we need veterinary pathologist?
SP  - 153
EP  - 154
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2957
ER  - 

@conference{
author = "Aleksić-Kovačević, Sanja and Kukolj, Vladimir and Nešić, Slađan and Vučićević, Ivana and Aničić, Milan and Marinković, Darko",
year = "2017",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "22. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 7-10.jun 2017",
title = "Koliko nam je neophodan veterinarski patolog?, Do we need veterinary pathologist?",
pages = "153-154",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2957"
}

Aleksić-Kovačević, S., Kukolj, V., Nešić, S., Vučićević, I., Aničić, M.,& Marinković, D.. (2017). Koliko nam je neophodan veterinarski patolog?. in 22. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 7-10.jun 2017
Banja Luka : Veterinarska komora Republike Srpske., 153-154.
https://hdl.handle.net/21.15107/rcub_veterinar_2957

Aleksić-Kovačević S, Kukolj V, Nešić S, Vučićević I, Aničić M, Marinković D. Koliko nam je neophodan veterinarski patolog?. in 22. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 7-10.jun 2017. 2017;:153-154.
https://hdl.handle.net/21.15107/rcub_veterinar_2957 .

Aleksić-Kovačević, Sanja, Kukolj, Vladimir, Nešić, Slađan, Vučićević, Ivana, Aničić, Milan, Marinković, Darko, "Koliko nam je neophodan veterinarski patolog?" in 22. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) sa međunarodnim učešćem, Teslić, 7-10.jun 2017 (2017):153-154,
https://hdl.handle.net/21.15107/rcub_veterinar_2957 .

TY  - JOUR
AU  - Marinković, Darko
AU  - Ozvegy, Jozsef
AU  - Aničić, Milan
AU  - Vučićević, Ivana
AU  - Nešić, Slađan
AU  - Kukolj, Vladimir
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1357
AB  - Gastric dilatation and volvulus is a life-threatening condition characterized by rapid accumulation of food and gases that cause displacement and distension of the stomach. The large and giant, deep-chested breeds of dogs are at higher risk for developing the gastric dilatation and volvulus. Uncommonly, it can also develop in cats, but it is also described in free-range polar bears. A case of gastric dilatation and volvulus in a brown bear (Ursus arctos) is described in this paper. This case was characterized by lack of any previous symptoms, sudden death, as well as macroscopic findings during necropsy - twisted distended stomach, congested displaced spleen and necrotic gastric wall. According to the available data this is the first described case report of the gastric dilatation and volvulus in brown bear (Ursus arctos).
AB  - Dilatacija i volvulus želuca je stanje opasno po život koje se karakteriše brzim nakupljanjem hrane i gasova dovodeći do pomeranja i rastezanja želuca. Psi velikih i gigantskih rasa sa dubokim grudnim košem su pod većim rizikom za razvoj ovog poremećaja. Veoma retko, dilatacija i torzija želuca može nastati i kod mačaka, a opisan je i slučaj kod polarnog medveda. U ovom radu je prikazan slučaj dilatacije i volvulusa želuca kod mrkog medveda (Ursus arctos). Izostanak simptoma, iznenadno uginuće, kao i makroskopski nalaz tokom obdukcije - uvrnut, proširen želudac, izmeštena kongestirana slezina i nekrotičan zid želuca su glavne karakteristike slučaja. Prema raspoloživim podacima ovo je prvi prikaz slučaja dilatacije i volvulusa želuca kod mrkog medveda (Ursus arctos).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Gastric dilatation and volvulus in brown bear (Ursus arctos)
T1  - Dilatacija i volvulus želuca kod mrkog medveda (Ursus arctos)
VL  - 66
IS  - 3
SP  - 422
EP  - 428
DO  - 10.1515/acve-2016-0036
ER  - 

@article{
author = "Marinković, Darko and Ozvegy, Jozsef and Aničić, Milan and Vučićević, Ivana and Nešić, Slađan and Kukolj, Vladimir",
year = "2016",
abstract = "Gastric dilatation and volvulus is a life-threatening condition characterized by rapid accumulation of food and gases that cause displacement and distension of the stomach. The large and giant, deep-chested breeds of dogs are at higher risk for developing the gastric dilatation and volvulus. Uncommonly, it can also develop in cats, but it is also described in free-range polar bears. A case of gastric dilatation and volvulus in a brown bear (Ursus arctos) is described in this paper. This case was characterized by lack of any previous symptoms, sudden death, as well as macroscopic findings during necropsy - twisted distended stomach, congested displaced spleen and necrotic gastric wall. According to the available data this is the first described case report of the gastric dilatation and volvulus in brown bear (Ursus arctos)., Dilatacija i volvulus želuca je stanje opasno po život koje se karakteriše brzim nakupljanjem hrane i gasova dovodeći do pomeranja i rastezanja želuca. Psi velikih i gigantskih rasa sa dubokim grudnim košem su pod većim rizikom za razvoj ovog poremećaja. Veoma retko, dilatacija i torzija želuca može nastati i kod mačaka, a opisan je i slučaj kod polarnog medveda. U ovom radu je prikazan slučaj dilatacije i volvulusa želuca kod mrkog medveda (Ursus arctos). Izostanak simptoma, iznenadno uginuće, kao i makroskopski nalaz tokom obdukcije - uvrnut, proširen želudac, izmeštena kongestirana slezina i nekrotičan zid želuca su glavne karakteristike slučaja. Prema raspoloživim podacima ovo je prvi prikaz slučaja dilatacije i volvulusa želuca kod mrkog medveda (Ursus arctos).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Gastric dilatation and volvulus in brown bear (Ursus arctos), Dilatacija i volvulus želuca kod mrkog medveda (Ursus arctos)",
volume = "66",
number = "3",
pages = "422-428",
doi = "10.1515/acve-2016-0036"
}

Marinković, D., Ozvegy, J., Aničić, M., Vučićević, I., Nešić, S.,& Kukolj, V.. (2016). Gastric dilatation and volvulus in brown bear (Ursus arctos). in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3), 422-428.
https://doi.org/10.1515/acve-2016-0036

Marinković D, Ozvegy J, Aničić M, Vučićević I, Nešić S, Kukolj V. Gastric dilatation and volvulus in brown bear (Ursus arctos). in Acta Veterinaria-Beograd. 2016;66(3):422-428.
doi:10.1515/acve-2016-0036 .

Marinković, Darko, Ozvegy, Jozsef, Aničić, Milan, Vučićević, Ivana, Nešić, Slađan, Kukolj, Vladimir, "Gastric dilatation and volvulus in brown bear (Ursus arctos)" in Acta Veterinaria-Beograd, 66, no. 3 (2016):422-428,
https://doi.org/10.1515/acve-2016-0036 . .

TY  - JOUR
AU  - Vučićević, Ivana
AU  - Marinković, Darko
AU  - Kukolj, Vladimir
AU  - Vučićević, Miloš
AU  - Mirilović, Milorad
AU  - Nešić, Slađan
AU  - Aleksić-Kovačević, Sanja
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1361
AB  - Histopathological examination, grading, immunohistochemical staining and molecular genetic examinations are the proposed criteria that should be used for cutaneous mast cell tumors (CMCTs) classification. The presence of aberrant CD117 expression and mutations of the c-kit proto-oncogene could be an indicative parameter for final histological grading. Determination of the connection between the localization of KIT receptor expression and the histological grade of CMCTs without c-kit proto-oncogene mutations was the main goal of this study. The study included twenty four CMCTs and six control skin samples from 30 dogs of different ages, breed and sex. Formalinfixed and paraffin-embedded tissue samples were stained with hematoxylin-eosin and toluidine blue and immunohistochemically tested for CD117 expression. DNA was extracted from the same paraffin blocks and subsequent polymerase chain reaction amplification was performed using PE1 and PE2 primers. Degree of malignancy was determined based on the presence of mitotic figures, multinucleated cells, bizarre nuclei and karyomegaly in 10 high power fields. Based on histological features, fourteen of 24 CMCTs were of a high histological grade, while ten were classified as a lowgrade malignancy. CD117 cytoplasmic expression was observed in nine of fourteen high-grade malignancy CMCTs, which confirms the link between the aberrant CD117 expression and increased cell proliferation.
AB  - Histopatološko ispitivanje, gradacija, imunohistohemijsko bojenje i molekularno genetska ispitivanja su predloženi kriterijumi za klasifikaciju kutanih mastocitoma pasa. Prisustvo aberantne ekspresije CD117 i mutacija c-kit protoonkogena moglo bi da bude indikativni parameter za finalnu histopatološku klasifikaciju mastocitoma. Glavni cilj ispitivanja je određivanje povezanosti mesta ekspresije KIT receptora i histološkog gradusa mastocitoma bez c-kit mutacija. Ispitivanjima su obuhvaćena 24 mastocitoma i 6 kontrolnih isečaka kože uzorkovanih od ukupno 30 odraslih pasa različitog uzrasta, rase i pola. Uzorci fiksirani u formalinu i ukalupljeni u parafinske kalupe su bojeni hematoksilin-eozinom, toluidin plavo metodom i imunohistohemijski primenom CD117 antitela. DNK je izolovana iz istih parafinskih kalupa, a PCR amplifikacija je izvršena primenom specifičnih prajmera PE1 i PE2. Stepen malignosti je utvrđen na osnovu prisustva mitotskih figura, multinuklearnih ćelija, ćelija sa bizarnim jedrom i kariomegalije na 10 polja velikog uveličanja. Na osnovu histopatoloških osobina, 14 od 24 mastocitoma su svrstani u visoki stepen maligniteta, dok je 10 mastocitoma niskog stepena maligniteta. Citoplazmatska ekspresija CD117 je uočena kod 9 od 14 mastocitoma visokog stepena maligniteta, što potvrđuje vezu između aberantne ekspresije CD117 i povećane ćelijske proliferacije.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - KIT receptor expression in canine cutaneous mast cell tumors (CMCTS) without c-kit mutation
T1  - Ekspresija KIT receptora na kutanim mastocitomima pasa bez c-kit mutacija
VL  - 66
IS  - 2
SP  - 222
EP  - 233
DO  - 10.1515/acve-2016-0019
ER  - 

@article{
author = "Vučićević, Ivana and Marinković, Darko and Kukolj, Vladimir and Vučićević, Miloš and Mirilović, Milorad and Nešić, Slađan and Aleksić-Kovačević, Sanja",
year = "2016",
abstract = "Histopathological examination, grading, immunohistochemical staining and molecular genetic examinations are the proposed criteria that should be used for cutaneous mast cell tumors (CMCTs) classification. The presence of aberrant CD117 expression and mutations of the c-kit proto-oncogene could be an indicative parameter for final histological grading. Determination of the connection between the localization of KIT receptor expression and the histological grade of CMCTs without c-kit proto-oncogene mutations was the main goal of this study. The study included twenty four CMCTs and six control skin samples from 30 dogs of different ages, breed and sex. Formalinfixed and paraffin-embedded tissue samples were stained with hematoxylin-eosin and toluidine blue and immunohistochemically tested for CD117 expression. DNA was extracted from the same paraffin blocks and subsequent polymerase chain reaction amplification was performed using PE1 and PE2 primers. Degree of malignancy was determined based on the presence of mitotic figures, multinucleated cells, bizarre nuclei and karyomegaly in 10 high power fields. Based on histological features, fourteen of 24 CMCTs were of a high histological grade, while ten were classified as a lowgrade malignancy. CD117 cytoplasmic expression was observed in nine of fourteen high-grade malignancy CMCTs, which confirms the link between the aberrant CD117 expression and increased cell proliferation., Histopatološko ispitivanje, gradacija, imunohistohemijsko bojenje i molekularno genetska ispitivanja su predloženi kriterijumi za klasifikaciju kutanih mastocitoma pasa. Prisustvo aberantne ekspresije CD117 i mutacija c-kit protoonkogena moglo bi da bude indikativni parameter za finalnu histopatološku klasifikaciju mastocitoma. Glavni cilj ispitivanja je određivanje povezanosti mesta ekspresije KIT receptora i histološkog gradusa mastocitoma bez c-kit mutacija. Ispitivanjima su obuhvaćena 24 mastocitoma i 6 kontrolnih isečaka kože uzorkovanih od ukupno 30 odraslih pasa različitog uzrasta, rase i pola. Uzorci fiksirani u formalinu i ukalupljeni u parafinske kalupe su bojeni hematoksilin-eozinom, toluidin plavo metodom i imunohistohemijski primenom CD117 antitela. DNK je izolovana iz istih parafinskih kalupa, a PCR amplifikacija je izvršena primenom specifičnih prajmera PE1 i PE2. Stepen malignosti je utvrđen na osnovu prisustva mitotskih figura, multinuklearnih ćelija, ćelija sa bizarnim jedrom i kariomegalije na 10 polja velikog uveličanja. Na osnovu histopatoloških osobina, 14 od 24 mastocitoma su svrstani u visoki stepen maligniteta, dok je 10 mastocitoma niskog stepena maligniteta. Citoplazmatska ekspresija CD117 je uočena kod 9 od 14 mastocitoma visokog stepena maligniteta, što potvrđuje vezu između aberantne ekspresije CD117 i povećane ćelijske proliferacije.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "KIT receptor expression in canine cutaneous mast cell tumors (CMCTS) without c-kit mutation, Ekspresija KIT receptora na kutanim mastocitomima pasa bez c-kit mutacija",
volume = "66",
number = "2",
pages = "222-233",
doi = "10.1515/acve-2016-0019"
}

Vučićević, I., Marinković, D., Kukolj, V., Vučićević, M., Mirilović, M., Nešić, S.,& Aleksić-Kovačević, S.. (2016). KIT receptor expression in canine cutaneous mast cell tumors (CMCTS) without c-kit mutation. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(2), 222-233.
https://doi.org/10.1515/acve-2016-0019

Vučićević I, Marinković D, Kukolj V, Vučićević M, Mirilović M, Nešić S, Aleksić-Kovačević S. KIT receptor expression in canine cutaneous mast cell tumors (CMCTS) without c-kit mutation. in Acta Veterinaria-Beograd. 2016;66(2):222-233.
doi:10.1515/acve-2016-0019 .

Vučićević, Ivana, Marinković, Darko, Kukolj, Vladimir, Vučićević, Miloš, Mirilović, Milorad, Nešić, Slađan, Aleksić-Kovačević, Sanja, "KIT receptor expression in canine cutaneous mast cell tumors (CMCTS) without c-kit mutation" in Acta Veterinaria-Beograd, 66, no. 2 (2016):222-233,
https://doi.org/10.1515/acve-2016-0019 . .

TY  - JOUR
AU  - Maletić, Jelena
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Maletić, Milan
AU  - Lakić, Nada
AU  - Kukolj, Vladimir
AU  - Aleksić, Nevenka
AU  - Anđelković, Marko
AU  - Stanimirović, Zoran
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1183
AB  - Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available published data originate from the single-cell gel electrophoresis (Comet) assay. Therefore, the objective of this study was to examine whether it can induce primary DNA damage using in vitro Comet assay in isolated rat lymphocytes. Lymphocytes were treated with a broad spectrum of tulathromycin concentrations (from 1 to 100 mu M) and co-treatment with an antioxidant, catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations of tulathromycin (50 and 100 mu M) caused significant increase of DNA damage in rat lymphocytes and catalase did not significantly reduce the DNA-damaging effect of tulathromycin. The results of this study indicate that tulathromycin induces genotoxic effects at high concentrations, that catalase does not exert protective effect in this case.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro
VL  - 47
IS  - 1
SP  - 339
EP  - 348
DO  - 10.2298/GENSR1501339M
ER  - 

@article{
author = "Maletić, Jelena and Đelić, Ninoslav and Radaković, Milena and Maletić, Milan and Lakić, Nada and Kukolj, Vladimir and Aleksić, Nevenka and Anđelković, Marko and Stanimirović, Zoran",
year = "2015",
abstract = "Tulathromycin is a relatively new semi-synthetic macrolide antibiotic, a member of the triamilide group, approved primarly for the treatment of respiratory diseases in cattle and swine. Various genotoxicological studies indicated that tulathromycin is not genotoxic, but no available published data originate from the single-cell gel electrophoresis (Comet) assay. Therefore, the objective of this study was to examine whether it can induce primary DNA damage using in vitro Comet assay in isolated rat lymphocytes. Lymphocytes were treated with a broad spectrum of tulathromycin concentrations (from 1 to 100 mu M) and co-treatment with an antioxidant, catalase (100 IU/mL and 500 IU/mL) was performed. The highest concentrations of tulathromycin (50 and 100 mu M) caused significant increase of DNA damage in rat lymphocytes and catalase did not significantly reduce the DNA-damaging effect of tulathromycin. The results of this study indicate that tulathromycin induces genotoxic effects at high concentrations, that catalase does not exert protective effect in this case.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro",
volume = "47",
number = "1",
pages = "339-348",
doi = "10.2298/GENSR1501339M"
}

Maletić, J., Đelić, N., Radaković, M., Maletić, M., Lakić, N., Kukolj, V., Aleksić, N., Anđelković, M.,& Stanimirović, Z.. (2015). Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro. in Genetika
Društvo genetičara Srbije, Beograd., 47(1), 339-348.
https://doi.org/10.2298/GENSR1501339M

Maletić J, Đelić N, Radaković M, Maletić M, Lakić N, Kukolj V, Aleksić N, Anđelković M, Stanimirović Z. Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro. in Genetika. 2015;47(1):339-348.
doi:10.2298/GENSR1501339M .

Maletić, Jelena, Đelić, Ninoslav, Radaković, Milena, Maletić, Milan, Lakić, Nada, Kukolj, Vladimir, Aleksić, Nevenka, Anđelković, Marko, Stanimirović, Zoran, "Evaluation of DNA damage in rat lymphocytes exposed to tulathromycin in vitro" in Genetika, 47, no. 1 (2015):339-348,
https://doi.org/10.2298/GENSR1501339M . .

TY  - JOUR
AU  - Kukolj, Vladimir
AU  - Aleksić-Kovačević, Sanja
AU  - Katić-Radivojević, Sofija
AU  - Knežević, Dj.
AU  - Jovanović, M.
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1173
AB  - The main objective of our research was to examine the role and immunophenotypic characteristics of myofibroblasts in sheep liver naturally infected by the lancet liver fluke (Dicrocoelium dendriticum). In the reported study we analyzed liver samples from 20 adult sheep, 14 infected animals and 6 controls. The liver samples were fixed in 10% buffered formalin, and routinely processed and stained using hematoxylin eosin, the periodic acid-Schiff and Masson-Goldner trichrome methods. The immunohistochemical examination was carried out by the streptavidin biotin (LSAB2) method, using antibodies for alpha-smooth muscle actin (alpha-SMA), desmin and vimentin. The histopathological examination revealed liver fibrosis in 6 out of 14 (42.9%) analyzed samples, while different forms of cholangitis were observed in the remaining 8 out of 14(57.1%). The expression of alpha-SMA was proven in perisinusoidal hepatic stellate cells, portal/septal myofibroblasts, and interface myofibroblasts. The degree of alpha-SMA expression and the number of alpha-SMA immunopositive cells were the most intensive in the liver with fibrosis. Desmin expression in all liver samples of infected sheep was confirmed in hepatic stellate cells and smooth muscle cells. The hepatic stellate cells, portal/septal myofibroblasts, and interface myofibroblasts reacted as vimentin positive cells. In the liver without fibrotic changes hepatic stellate cells and smooth muscle cells were desmin positive. The obtained results suggest that all populations of myofibroblasts, especially hepatic stellate cells, play an important role in the increased extracellular matrix formation during parasitic liver fibrosis in sheep naturally infected with D. dendriticum.
PB  - Elsevier Science Bv, Amsterdam
T2  - Veterinary Parasitology
T1  - The role and immunophenotypic characteristics of myofibroblasts in liver of sheep naturally infected with the lancet liver fluke (Dicrocoelium dendriticum)
VL  - 208
IS  - 3-4
SP  - 181
EP  - 189
DO  - 10.1016/j.vetpar.2015.01.022
ER  - 

@article{
author = "Kukolj, Vladimir and Aleksić-Kovačević, Sanja and Katić-Radivojević, Sofija and Knežević, Dj. and Jovanović, M.",
year = "2015",
abstract = "The main objective of our research was to examine the role and immunophenotypic characteristics of myofibroblasts in sheep liver naturally infected by the lancet liver fluke (Dicrocoelium dendriticum). In the reported study we analyzed liver samples from 20 adult sheep, 14 infected animals and 6 controls. The liver samples were fixed in 10% buffered formalin, and routinely processed and stained using hematoxylin eosin, the periodic acid-Schiff and Masson-Goldner trichrome methods. The immunohistochemical examination was carried out by the streptavidin biotin (LSAB2) method, using antibodies for alpha-smooth muscle actin (alpha-SMA), desmin and vimentin. The histopathological examination revealed liver fibrosis in 6 out of 14 (42.9%) analyzed samples, while different forms of cholangitis were observed in the remaining 8 out of 14(57.1%). The expression of alpha-SMA was proven in perisinusoidal hepatic stellate cells, portal/septal myofibroblasts, and interface myofibroblasts. The degree of alpha-SMA expression and the number of alpha-SMA immunopositive cells were the most intensive in the liver with fibrosis. Desmin expression in all liver samples of infected sheep was confirmed in hepatic stellate cells and smooth muscle cells. The hepatic stellate cells, portal/septal myofibroblasts, and interface myofibroblasts reacted as vimentin positive cells. In the liver without fibrotic changes hepatic stellate cells and smooth muscle cells were desmin positive. The obtained results suggest that all populations of myofibroblasts, especially hepatic stellate cells, play an important role in the increased extracellular matrix formation during parasitic liver fibrosis in sheep naturally infected with D. dendriticum.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Veterinary Parasitology",
title = "The role and immunophenotypic characteristics of myofibroblasts in liver of sheep naturally infected with the lancet liver fluke (Dicrocoelium dendriticum)",
volume = "208",
number = "3-4",
pages = "181-189",
doi = "10.1016/j.vetpar.2015.01.022"
}

Kukolj, V., Aleksić-Kovačević, S., Katić-Radivojević, S., Knežević, Dj.,& Jovanović, M.. (2015). The role and immunophenotypic characteristics of myofibroblasts in liver of sheep naturally infected with the lancet liver fluke (Dicrocoelium dendriticum). in Veterinary Parasitology
Elsevier Science Bv, Amsterdam., 208(3-4), 181-189.
https://doi.org/10.1016/j.vetpar.2015.01.022

Kukolj V, Aleksić-Kovačević S, Katić-Radivojević S, Knežević D, Jovanović M. The role and immunophenotypic characteristics of myofibroblasts in liver of sheep naturally infected with the lancet liver fluke (Dicrocoelium dendriticum). in Veterinary Parasitology. 2015;208(3-4):181-189.
doi:10.1016/j.vetpar.2015.01.022 .

Kukolj, Vladimir, Aleksić-Kovačević, Sanja, Katić-Radivojević, Sofija, Knežević, Dj., Jovanović, M., "The role and immunophenotypic characteristics of myofibroblasts in liver of sheep naturally infected with the lancet liver fluke (Dicrocoelium dendriticum)" in Veterinary Parasitology, 208, no. 3-4 (2015):181-189,
https://doi.org/10.1016/j.vetpar.2015.01.022 . .

TY  - JOUR
AU  - Kukolj, Vladimir
AU  - Nešić, Slađan
AU  - Vučićević, Ivana
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1219
AB  - Increasing of the extracellular matrix in rats, as well as in humans, occurs as a consequence of hepatic stellate cells (HSCs) activity. The objective of this work was to investigation the role of these cells in the development of fibrosis and liver cirrhosis which occurs as a consequence of infection of sheep and goats with large (Fasciola hepatica) and small (Dicrocoelium dendriticum) fluke. Liver samples taken from 12 cattle and 10 sheep infected under natural conditions with large and small fluke were fixed in formalin and embedded in paraffin. Paraffin clips were stained with hematoxylin- eosin and masson trichrome method, and immunohistochemical method for α-smooth muscle actin (α-SMA). All tested samples were divided into three groups according to histological criteria: livers of infected animals with the first degree of fibrosis, livers of infected animals with the second degree of fibrosis, and livers of infected animals with cirrhosis. Distribution of HSCs depended on the degree of liver fibrosis. Immunohistochemically reactive HSCs were predominantly placed in perisinusoidal space. In liver samples with cirrhosis, HSCs were placed on the periphery of pseudolobulus. Cells of a different shape and size were positive to α-SMA. HSCs play an important role in synthesis of components of extracellular matrix during the development of parasitic fibrosis and liver cirrhosis in domestic animals.
AB  - Povećanje ekstracelularnog matriksa kod pacova, kao i kod ljudi, nastaje kao posledica aktivacije zvezdastih ćelija jetre (HSCs). Cilj ovog rada je izučavanje uloge ovih ćelija u razvoju fibroze i ciroze jetre koje nastaju kao posledice infekcija ovaca i goveda velikim (Fasciola hepatica) i malim (Dicrocoelium dendriticum) metiljem. Uzorci jetri 12 goveda i 10 ovaca koje su u prirodinim uslovima inficirane velikim i malim metiljem su fiksirani u formalinu i uklopljeni u parafin. Parafinski isečci su bojeni hematoksilin-eozinom i Mason-trihromnom metodom, kao i imunohistohemijskom metodom za α-glatko-mišićni aktin (α-SMA). Svi ispitani uzorci su prema histološkim kriterijumima podeljeni u tri grupe: jetre inficiranih životinja sa I stepenom fibroze, jetre inficiranih životinja sa II stepenom fibroze i jetre inficiranih životinja sa cirozom. Distribucija HSCs je zavisila od stepena fibroze jetre. Imunohistohemijski reaktivne HSCs su bile predominantno smeštene u perisinusoidnim prostorima. U uzorcima jetre sa cirozom HSCs su bile smeštene na periferiji pseudolobulusa. Ćelije različitog oblika i veličine su bile pozitivne na α-SMA. HSCs igraju važnu ulogu u sintezi komponenti ekstracelularnog matriksa tokom razvoja parazitske fibroze i ciroze jetre kod domaćih životinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Distribution of hepatic stellate cells and their role in the development of parasitic fibrosis and liver cirrhosis in domestic animals
T1  - Distribucija zvezdastih ćelija jetre i njihova uloga u nastanku parazitske fibroze i ciroze jetre kod domaćih životinja
VL  - 69
IS  - 1-2
SP  - 3
EP  - 11
DO  - 10.2298/VETGL1502003K
ER  - 

@article{
author = "Kukolj, Vladimir and Nešić, Slađan and Vučićević, Ivana",
year = "2015",
abstract = "Increasing of the extracellular matrix in rats, as well as in humans, occurs as a consequence of hepatic stellate cells (HSCs) activity. The objective of this work was to investigation the role of these cells in the development of fibrosis and liver cirrhosis which occurs as a consequence of infection of sheep and goats with large (Fasciola hepatica) and small (Dicrocoelium dendriticum) fluke. Liver samples taken from 12 cattle and 10 sheep infected under natural conditions with large and small fluke were fixed in formalin and embedded in paraffin. Paraffin clips were stained with hematoxylin- eosin and masson trichrome method, and immunohistochemical method for α-smooth muscle actin (α-SMA). All tested samples were divided into three groups according to histological criteria: livers of infected animals with the first degree of fibrosis, livers of infected animals with the second degree of fibrosis, and livers of infected animals with cirrhosis. Distribution of HSCs depended on the degree of liver fibrosis. Immunohistochemically reactive HSCs were predominantly placed in perisinusoidal space. In liver samples with cirrhosis, HSCs were placed on the periphery of pseudolobulus. Cells of a different shape and size were positive to α-SMA. HSCs play an important role in synthesis of components of extracellular matrix during the development of parasitic fibrosis and liver cirrhosis in domestic animals., Povećanje ekstracelularnog matriksa kod pacova, kao i kod ljudi, nastaje kao posledica aktivacije zvezdastih ćelija jetre (HSCs). Cilj ovog rada je izučavanje uloge ovih ćelija u razvoju fibroze i ciroze jetre koje nastaju kao posledice infekcija ovaca i goveda velikim (Fasciola hepatica) i malim (Dicrocoelium dendriticum) metiljem. Uzorci jetri 12 goveda i 10 ovaca koje su u prirodinim uslovima inficirane velikim i malim metiljem su fiksirani u formalinu i uklopljeni u parafin. Parafinski isečci su bojeni hematoksilin-eozinom i Mason-trihromnom metodom, kao i imunohistohemijskom metodom za α-glatko-mišićni aktin (α-SMA). Svi ispitani uzorci su prema histološkim kriterijumima podeljeni u tri grupe: jetre inficiranih životinja sa I stepenom fibroze, jetre inficiranih životinja sa II stepenom fibroze i jetre inficiranih životinja sa cirozom. Distribucija HSCs je zavisila od stepena fibroze jetre. Imunohistohemijski reaktivne HSCs su bile predominantno smeštene u perisinusoidnim prostorima. U uzorcima jetre sa cirozom HSCs su bile smeštene na periferiji pseudolobulusa. Ćelije različitog oblika i veličine su bile pozitivne na α-SMA. HSCs igraju važnu ulogu u sintezi komponenti ekstracelularnog matriksa tokom razvoja parazitske fibroze i ciroze jetre kod domaćih životinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Distribution of hepatic stellate cells and their role in the development of parasitic fibrosis and liver cirrhosis in domestic animals, Distribucija zvezdastih ćelija jetre i njihova uloga u nastanku parazitske fibroze i ciroze jetre kod domaćih životinja",
volume = "69",
number = "1-2",
pages = "3-11",
doi = "10.2298/VETGL1502003K"
}

Kukolj, V., Nešić, S.,& Vučićević, I.. (2015). Distribution of hepatic stellate cells and their role in the development of parasitic fibrosis and liver cirrhosis in domestic animals. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(1-2), 3-11.
https://doi.org/10.2298/VETGL1502003K

Kukolj V, Nešić S, Vučićević I. Distribution of hepatic stellate cells and their role in the development of parasitic fibrosis and liver cirrhosis in domestic animals. in Veterinarski Glasnik. 2015;69(1-2):3-11.
doi:10.2298/VETGL1502003K .

Kukolj, Vladimir, Nešić, Slađan, Vučićević, Ivana, "Distribution of hepatic stellate cells and their role in the development of parasitic fibrosis and liver cirrhosis in domestic animals" in Veterinarski Glasnik, 69, no. 1-2 (2015):3-11,
https://doi.org/10.2298/VETGL1502003K . .