Pajić, Marija

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orcid::0000-0001-9756-2461
  • Pajić, Marija (7)
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Author's Bibliography

Looking for added value of milk products of autochthonous sheep breeds

Becskei, Zsolt; Paskaš, Snežana; Savić, Mila; Tarić, Elmin; Gáspárdy, András; Pajić, Marija; Rašeta, Mladen; Lazarova, Mariya

(DAGENE - International Association for the Conservation of Animal Breeds in the Danube region, 2023)

TY  - JOUR
AU  - Becskei, Zsolt
AU  - Paskaš, Snežana
AU  - Savić, Mila
AU  - Tarić, Elmin
AU  - Gáspárdy, András
AU  - Pajić, Marija
AU  - Rašeta, Mladen
AU  - Lazarova, Mariya
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3379
AB  - In order to determine the added value of the product, characterization of sheep milk
and cheese samples from Sjenica sheep were assessed and compared to British milk
sheep. The analyses were carried out according to standard chemical procedures. A
comparative study showed that the milk from the Sjenica sheep was higher in fat and
protein and had a lower casein/fat ratio. It also had higher total and somatic cell
counts than British milk sheep. Sjenica cheese had significantly higher dry matter
and fat content. However, both cheeses were classified as soft full-fat cheese with
brine. The study showed that sheep's milk contains many essential components for
cheesemaking and distinguishes sheep's cheese from other types of cheese, but
standardisation of cheese production is still needed. To preserve the autochthonous
sheep breeds of Serbia, promoting cheese production with added value is crucial and
together with increased production could contribute to the sustainable development
of rural areas
PB  - DAGENE - International Association for the Conservation of Animal Breeds in the Danube region
T2  - Danubian Animal Genetic Resources
T1  - Looking for added value of milk products of autochthonous sheep breeds
VL  - 8
IS  - 1
SP  - 29
EP  - 36
DO  - 10.59913/dagr.2023.12257
ER  - 
@article{
author = "Becskei, Zsolt and Paskaš, Snežana and Savić, Mila and Tarić, Elmin and Gáspárdy, András and Pajić, Marija and Rašeta, Mladen and Lazarova, Mariya",
year = "2023",
abstract = "In order to determine the added value of the product, characterization of sheep milk
and cheese samples from Sjenica sheep were assessed and compared to British milk
sheep. The analyses were carried out according to standard chemical procedures. A
comparative study showed that the milk from the Sjenica sheep was higher in fat and
protein and had a lower casein/fat ratio. It also had higher total and somatic cell
counts than British milk sheep. Sjenica cheese had significantly higher dry matter
and fat content. However, both cheeses were classified as soft full-fat cheese with
brine. The study showed that sheep's milk contains many essential components for
cheesemaking and distinguishes sheep's cheese from other types of cheese, but
standardisation of cheese production is still needed. To preserve the autochthonous
sheep breeds of Serbia, promoting cheese production with added value is crucial and
together with increased production could contribute to the sustainable development
of rural areas",
publisher = "DAGENE - International Association for the Conservation of Animal Breeds in the Danube region",
journal = "Danubian Animal Genetic Resources",
title = "Looking for added value of milk products of autochthonous sheep breeds",
volume = "8",
number = "1",
pages = "29-36",
doi = "10.59913/dagr.2023.12257"
}
Becskei, Z., Paskaš, S., Savić, M., Tarić, E., Gáspárdy, A., Pajić, M., Rašeta, M.,& Lazarova, M.. (2023). Looking for added value of milk products of autochthonous sheep breeds. in Danubian Animal Genetic Resources
DAGENE - International Association for the Conservation of Animal Breeds in the Danube region., 8(1), 29-36.
https://doi.org/10.59913/dagr.2023.12257
Becskei Z, Paskaš S, Savić M, Tarić E, Gáspárdy A, Pajić M, Rašeta M, Lazarova M. Looking for added value of milk products of autochthonous sheep breeds. in Danubian Animal Genetic Resources. 2023;8(1):29-36.
doi:10.59913/dagr.2023.12257 .
Becskei, Zsolt, Paskaš, Snežana, Savić, Mila, Tarić, Elmin, Gáspárdy, András, Pajić, Marija, Rašeta, Mladen, Lazarova, Mariya, "Looking for added value of milk products of autochthonous sheep breeds" in Danubian Animal Genetic Resources, 8, no. 1 (2023):29-36,
https://doi.org/10.59913/dagr.2023.12257 . .

Proizvodnja magarećeg mleka – mogućnosti i izazovi

Pajić, Marija; Čobanović, Ksenija; Erdeljan, Mihajlo; Bečkei, Žolt; Čorba, Čaba; Kukurić, Tijana; Rekecki, Tibor

(Banja Luka : Veterinarska komora Republike Srpske, 2023)

TY  - CONF
AU  - Pajić, Marija
AU  - Čobanović, Ksenija
AU  - Erdeljan, Mihajlo
AU  - Bečkei, Žolt
AU  - Čorba, Čaba
AU  - Kukurić, Tijana
AU  - Rekecki, Tibor
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3081
AB  - Козметичко и лековито дејство магарећег млека познато је хиљадама година.
Циљ овог истраживања био је да се утврди хемијски састав и хигијенска
исправност стадног млека балканских магарица добијеног ручном мужом са
три фарме магараца у Војводини, како би се сазнало више о нутритивним
својствима и безбедности магарећег млека. Осим тога, ово истраживање треба
да допринесе бољем разумевању проблема и изазова на фармама магараца и
да укаже на основне потребе потрошача магарећег млека. Домаћи балкански
магарац припада породици Equidae која спада у ред копитара (Perissodactyla),
узгајаних у брдско-планинским пределима Балканског полуострва. Магареће
млеко је комплексни раствор стотине компоненти које се могу поделити на
протеине, масти, угљене хидрате, минерале и витамине. Магареће млеко је по
свом хемијском саставу најсличније кобиљем и мајчином млеку, а значајно се
разликује од крављег, овчијег и козјег млека. У потрази за природним и
здравим начином живота људи се све више враћају традиционалним лековима
и храни, због чега се данас све чешће користи магареће млеко. Због тога се
отварају многе могућности, али и изазови које треба превазићи.
AB  - Cosmetic and health effects of donkey milk have been known for thousands of
years. The aim of this research was to determine the chemical composition and
hygienic suitability of the mixed milk of Balkan donkeys obtained by hand milking
from three donkey farms in Vojvodina, with the intention of learning more about
the nutritional properties and safety of donkey milk. In addition, this research
shall contribute to a better understanding of problems and challenges on donkey
farms and indicate the basic needs of donkey milk consumers. The native Balkan
donkey belongs to the Equidae family which is in the order of odd-toed ungulates
(Perissodactyla), bred in the hilly and mountainous areas of the Balkan Peninsula.
Donkey milk is a complex solution with hundreds of components that can be
divided into proteins, fats, carbohydrates, minerals and vitamins. In terms of its
chemical composition, donkey milk is most similar to mare milk and mother’s
milk, and is significantly different from cow’s, sheep’s and goat’s milk. In search
of a natural healthy lifestyle, people are rediscovering more and more traditional
medicines and foods, which is why donkey milk is nowdays used more and more
frequently. This opens up many opportunities, but also challenges that need to be
overcome.
PB  - Banja Luka : Veterinarska komora Republike Srpske
C3  - 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023
T1  - Proizvodnja magarećeg mleka – mogućnosti i izazovi
T1  - Donkey milk production – possibilities and challenges
SP  - 140
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3081
ER  - 
@conference{
author = "Pajić, Marija and Čobanović, Ksenija and Erdeljan, Mihajlo and Bečkei, Žolt and Čorba, Čaba and Kukurić, Tijana and Rekecki, Tibor",
year = "2023",
abstract = "Козметичко и лековито дејство магарећег млека познато је хиљадама година.
Циљ овог истраживања био је да се утврди хемијски састав и хигијенска
исправност стадног млека балканских магарица добијеног ручном мужом са
три фарме магараца у Војводини, како би се сазнало више о нутритивним
својствима и безбедности магарећег млека. Осим тога, ово истраживање треба
да допринесе бољем разумевању проблема и изазова на фармама магараца и
да укаже на основне потребе потрошача магарећег млека. Домаћи балкански
магарац припада породици Equidae која спада у ред копитара (Perissodactyla),
узгајаних у брдско-планинским пределима Балканског полуострва. Магареће
млеко је комплексни раствор стотине компоненти које се могу поделити на
протеине, масти, угљене хидрате, минерале и витамине. Магареће млеко је по
свом хемијском саставу најсличније кобиљем и мајчином млеку, а значајно се
разликује од крављег, овчијег и козјег млека. У потрази за природним и
здравим начином живота људи се све више враћају традиционалним лековима
и храни, због чега се данас све чешће користи магареће млеко. Због тога се
отварају многе могућности, али и изазови које треба превазићи., Cosmetic and health effects of donkey milk have been known for thousands of
years. The aim of this research was to determine the chemical composition and
hygienic suitability of the mixed milk of Balkan donkeys obtained by hand milking
from three donkey farms in Vojvodina, with the intention of learning more about
the nutritional properties and safety of donkey milk. In addition, this research
shall contribute to a better understanding of problems and challenges on donkey
farms and indicate the basic needs of donkey milk consumers. The native Balkan
donkey belongs to the Equidae family which is in the order of odd-toed ungulates
(Perissodactyla), bred in the hilly and mountainous areas of the Balkan Peninsula.
Donkey milk is a complex solution with hundreds of components that can be
divided into proteins, fats, carbohydrates, minerals and vitamins. In terms of its
chemical composition, donkey milk is most similar to mare milk and mother’s
milk, and is significantly different from cow’s, sheep’s and goat’s milk. In search
of a natural healthy lifestyle, people are rediscovering more and more traditional
medicines and foods, which is why donkey milk is nowdays used more and more
frequently. This opens up many opportunities, but also challenges that need to be
overcome.",
publisher = "Banja Luka : Veterinarska komora Republike Srpske",
journal = "28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023",
title = "Proizvodnja magarećeg mleka – mogućnosti i izazovi, Donkey milk production – possibilities and challenges",
pages = "140-141",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3081"
}
Pajić, M., Čobanović, K., Erdeljan, M., Bečkei, Ž., Čorba, Č., Kukurić, T.,& Rekecki, T.. (2023). Proizvodnja magarećeg mleka – mogućnosti i izazovi. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023
Banja Luka : Veterinarska komora Republike Srpske., 140-141.
https://hdl.handle.net/21.15107/rcub_veterinar_3081
Pajić M, Čobanović K, Erdeljan M, Bečkei Ž, Čorba Č, Kukurić T, Rekecki T. Proizvodnja magarećeg mleka – mogućnosti i izazovi. in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023. 2023;:140-141.
https://hdl.handle.net/21.15107/rcub_veterinar_3081 .
Pajić, Marija, Čobanović, Ksenija, Erdeljan, Mihajlo, Bečkei, Žolt, Čorba, Čaba, Kukurić, Tijana, Rekecki, Tibor, "Proizvodnja magarećeg mleka – mogućnosti i izazovi" in 28. Godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina), Trebinje, 15 - 17. jun 2023 (2023):140-141,
https://hdl.handle.net/21.15107/rcub_veterinar_3081 .

Assessment of water buffalo milk and traditional milk products in a sustainable production system

Becskei, Zsolt; Savić, Mila; Ćirković, Dragan; Rašeta, Mladen; Puvača, Nikola; Pajić, Marija; Đorđević, Sonja; Paskaš, Snežana

(MDPI, 2020)


                                            

                                            
Becskei, Z., Savić, M., Ćirković, D., Rašeta, M., Puvača, N., Pajić, M., Đorđević, S.,& Paskaš, S.. (2020). Assessment of water buffalo milk and traditional milk products in a sustainable production system. in Sustainability (Switzerland)
MDPI., 12(16).
https://hdl.handle.net/21.15107/rcub_veterinar_1977
Becskei Z, Savić M, Ćirković D, Rašeta M, Puvača N, Pajić M, Đorđević S, Paskaš S. Assessment of water buffalo milk and traditional milk products in a sustainable production system. in Sustainability (Switzerland). 2020;12(16).
https://hdl.handle.net/21.15107/rcub_veterinar_1977 .
Becskei, Zsolt, Savić, Mila, Ćirković, Dragan, Rašeta, Mladen, Puvača, Nikola, Pajić, Marija, Đorđević, Sonja, Paskaš, Snežana, "Assessment of water buffalo milk and traditional milk products in a sustainable production system" in Sustainability (Switzerland), 12, no. 16 (2020),
https://hdl.handle.net/21.15107/rcub_veterinar_1977 .

Consumer attitudes towards goat milk and goat milk products in Vojvodina

Paskaš, Snežana; Miočinović, Jelena; Lopičić-Vasić, Tijana; Mugoša, Izabela; Pajić, Marija; Becskei, Zsolt

(Hrvatska Mljekarska Udruga (Croatian Dairy Society), 2020)

TY  - JOUR
AU  - Paskaš, Snežana
AU  - Miočinović, Jelena
AU  - Lopičić-Vasić, Tijana
AU  - Mugoša, Izabela
AU  - Pajić, Marija
AU  - Becskei, Zsolt
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1848
AB  - This study presents the results of a survey conducted in the Province of Vojvodina, Serbia to investigate consumers’ attitudes towards goat milk and goat milk products. Furthermore, the factors affecting consumer perception and subjective norms with respect to purchase were examined. The quantitative random survey was conducted and the data were collected using a structured questionnaire. The findings from this study revealed significantly lower acceptance of goat milk and dairy products compared to bovine milk and dairy products. Of total 440 respondents, 24.2 % had never consumed goat milk with significant positive impact (p<0.05) of age on consumption was found. Among, respondents, mostly the young population (18-25 years) does not consume goat milk. The study identified the odour (42.3 %) and taste (22.5 %) as the main barriers for consuming goat milk. In regard to purchasing, more than half of interviewed respondents do not buy goat milk and dairy products (67 % and 70.5 %, respectively) while the rest respondents expressed very low purchasing frequency. Health benefits represent the most important reason for potential consuming goat milk and dairy products (66.5 %). On the other hand, the main motives for purchasing bovine milk and dairy products are taste and quality (55.5 %). In particular, the oldest surveyed population (40-55 years) seemed to be the most positive group toward goat milk and the most interested in the product’s nutritional value and its health impact. The main category of goat dairy products purchased among surveyed respondents was cheese. These findings suggest that for better positioning on the market and for producing price-competitive products goat milk and dairy products should be more clarified as either quality products or products with added value or as products with unique health benefits.
PB  - Hrvatska Mljekarska Udruga (Croatian Dairy Society)
T2  - Mljekarstvo
T1  - Consumer attitudes towards goat milk and goat milk products in Vojvodina
VL  - 70
IS  - 3
SP  - 171
EP  - 183
DO  - 10.15567/mljekarstvo.2020.0304
ER  - 
@article{
author = "Paskaš, Snežana and Miočinović, Jelena and Lopičić-Vasić, Tijana and Mugoša, Izabela and Pajić, Marija and Becskei, Zsolt",
year = "2020",
abstract = "This study presents the results of a survey conducted in the Province of Vojvodina, Serbia to investigate consumers’ attitudes towards goat milk and goat milk products. Furthermore, the factors affecting consumer perception and subjective norms with respect to purchase were examined. The quantitative random survey was conducted and the data were collected using a structured questionnaire. The findings from this study revealed significantly lower acceptance of goat milk and dairy products compared to bovine milk and dairy products. Of total 440 respondents, 24.2 % had never consumed goat milk with significant positive impact (p<0.05) of age on consumption was found. Among, respondents, mostly the young population (18-25 years) does not consume goat milk. The study identified the odour (42.3 %) and taste (22.5 %) as the main barriers for consuming goat milk. In regard to purchasing, more than half of interviewed respondents do not buy goat milk and dairy products (67 % and 70.5 %, respectively) while the rest respondents expressed very low purchasing frequency. Health benefits represent the most important reason for potential consuming goat milk and dairy products (66.5 %). On the other hand, the main motives for purchasing bovine milk and dairy products are taste and quality (55.5 %). In particular, the oldest surveyed population (40-55 years) seemed to be the most positive group toward goat milk and the most interested in the product’s nutritional value and its health impact. The main category of goat dairy products purchased among surveyed respondents was cheese. These findings suggest that for better positioning on the market and for producing price-competitive products goat milk and dairy products should be more clarified as either quality products or products with added value or as products with unique health benefits.",
publisher = "Hrvatska Mljekarska Udruga (Croatian Dairy Society)",
journal = "Mljekarstvo",
title = "Consumer attitudes towards goat milk and goat milk products in Vojvodina",
volume = "70",
number = "3",
pages = "171-183",
doi = "10.15567/mljekarstvo.2020.0304"
}
Paskaš, S., Miočinović, J., Lopičić-Vasić, T., Mugoša, I., Pajić, M.,& Becskei, Z.. (2020). Consumer attitudes towards goat milk and goat milk products in Vojvodina. in Mljekarstvo
Hrvatska Mljekarska Udruga (Croatian Dairy Society)., 70(3), 171-183.
https://doi.org/10.15567/mljekarstvo.2020.0304
Paskaš S, Miočinović J, Lopičić-Vasić T, Mugoša I, Pajić M, Becskei Z. Consumer attitudes towards goat milk and goat milk products in Vojvodina. in Mljekarstvo. 2020;70(3):171-183.
doi:10.15567/mljekarstvo.2020.0304 .
Paskaš, Snežana, Miočinović, Jelena, Lopičić-Vasić, Tijana, Mugoša, Izabela, Pajić, Marija, Becskei, Zsolt, "Consumer attitudes towards goat milk and goat milk products in Vojvodina" in Mljekarstvo, 70, no. 3 (2020):171-183,
https://doi.org/10.15567/mljekarstvo.2020.0304 . .
8
2
11

Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk

Babić, Milijana; Pajić, Marija; Radinović, Miodrag; Boboš, Stanko; Bulajić, Snežana; Nikolić, Aleksandra; Velebit, Branko

(Mary Ann Liebert, Inc, New Rochelle, 2019)

TY  - JOUR
AU  - Babić, Milijana
AU  - Pajić, Marija
AU  - Radinović, Miodrag
AU  - Boboš, Stanko
AU  - Bulajić, Snežana
AU  - Nikolić, Aleksandra
AU  - Velebit, Branko
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1784
AB  - The aim of this study was to determine the effects of different temperatures and storage time on Staphylococcus aureus growth, sea gene expression, and synthesis of staphylococcal enterotoxin A (SEA) in the pasteurized and UHT-pasteurized milk. Pasteurized and UHT-pasteurized milk were inoculated with 3.98 log(10) CFU/mL of S. aureus (ATCC 13565). Inoculated milk samples were stored at 8 degrees C, 15 degrees C, and 22 degrees C for 24, 48, and 72 h, respectively. SEA synthesis was detected with a fully automated miniVIDAS instrument using the Enzyme-Linked Fluorescent Assay (ELFA) technology. The patterns of gene regulation were detected by quantitative reverse transcriptase PCR. The 2(-Delta Delta CT) method has been used as a relative quantification strategy for gene expression responses data analysis. The results indicated that growth rate, sea gene expression, and SEA synthesis were influenced by type of milk, storage time, and temperature. Incubation of milk at different temperatures (15 degrees C and 22 degrees C) and times was used to simulate inadequate transport and storage conditions. Storage of pasteurized milk at 22 degrees C for 24 h significantly upregulated the expression of sea gene compared with milk stored at 8 degrees C, which coincides with the achieved S. aureus number of 10(5) CFU/mL and detected amount of SEA. In addition, storage of UHT-pasteurized milk at 22 degrees C for 24 h and at 15 degrees C for 48 h significantly upregulated the sea gene expression compared with milk stored at 8 degrees C, which coincides with the detected amount of SEA and the dynamics of S. aureus number change. It can, therefore, be concluded that implementing good hygiene practices to avoid pre- and post-heat treatment milk contamination and maintaining the cold chain at temperature <8 degrees C throughout the entire dairy production chain are of paramount importance to decrease the risk of staphylococcal food poisoning.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Foodborne Pathogens and Disease
T1  - Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk
VL  - 16
IS  - 4
SP  - 282
EP  - 289
DO  - 10.1089/fpd.2018.2544
ER  - 
@article{
author = "Babić, Milijana and Pajić, Marija and Radinović, Miodrag and Boboš, Stanko and Bulajić, Snežana and Nikolić, Aleksandra and Velebit, Branko",
year = "2019",
abstract = "The aim of this study was to determine the effects of different temperatures and storage time on Staphylococcus aureus growth, sea gene expression, and synthesis of staphylococcal enterotoxin A (SEA) in the pasteurized and UHT-pasteurized milk. Pasteurized and UHT-pasteurized milk were inoculated with 3.98 log(10) CFU/mL of S. aureus (ATCC 13565). Inoculated milk samples were stored at 8 degrees C, 15 degrees C, and 22 degrees C for 24, 48, and 72 h, respectively. SEA synthesis was detected with a fully automated miniVIDAS instrument using the Enzyme-Linked Fluorescent Assay (ELFA) technology. The patterns of gene regulation were detected by quantitative reverse transcriptase PCR. The 2(-Delta Delta CT) method has been used as a relative quantification strategy for gene expression responses data analysis. The results indicated that growth rate, sea gene expression, and SEA synthesis were influenced by type of milk, storage time, and temperature. Incubation of milk at different temperatures (15 degrees C and 22 degrees C) and times was used to simulate inadequate transport and storage conditions. Storage of pasteurized milk at 22 degrees C for 24 h significantly upregulated the expression of sea gene compared with milk stored at 8 degrees C, which coincides with the achieved S. aureus number of 10(5) CFU/mL and detected amount of SEA. In addition, storage of UHT-pasteurized milk at 22 degrees C for 24 h and at 15 degrees C for 48 h significantly upregulated the sea gene expression compared with milk stored at 8 degrees C, which coincides with the detected amount of SEA and the dynamics of S. aureus number change. It can, therefore, be concluded that implementing good hygiene practices to avoid pre- and post-heat treatment milk contamination and maintaining the cold chain at temperature <8 degrees C throughout the entire dairy production chain are of paramount importance to decrease the risk of staphylococcal food poisoning.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Foodborne Pathogens and Disease",
title = "Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk",
volume = "16",
number = "4",
pages = "282-289",
doi = "10.1089/fpd.2018.2544"
}
Babić, M., Pajić, M., Radinović, M., Boboš, S., Bulajić, S., Nikolić, A.,& Velebit, B.. (2019). Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk. in Foodborne Pathogens and Disease
Mary Ann Liebert, Inc, New Rochelle., 16(4), 282-289.
https://doi.org/10.1089/fpd.2018.2544
Babić M, Pajić M, Radinović M, Boboš S, Bulajić S, Nikolić A, Velebit B. Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk. in Foodborne Pathogens and Disease. 2019;16(4):282-289.
doi:10.1089/fpd.2018.2544 .
Babić, Milijana, Pajić, Marija, Radinović, Miodrag, Boboš, Stanko, Bulajić, Snežana, Nikolić, Aleksandra, Velebit, Branko, "Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk" in Foodborne Pathogens and Disease, 16, no. 4 (2019):282-289,
https://doi.org/10.1089/fpd.2018.2544 . .
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Expression of toxic shock syndrome toxin-1 gene of Staphylococcus aureus in milk: Proof of concept

Babić, Milijana; Pajić, Marija; Nikolić, Aleksandra; Teodorović, Vlado; Mirilović, Milorad; Milojević, Lazar; Velebit, Branko

(Croatian Dairy Union, Zagreb, 2018)

TY  - JOUR
AU  - Babić, Milijana
AU  - Pajić, Marija
AU  - Nikolić, Aleksandra
AU  - Teodorović, Vlado
AU  - Mirilović, Milorad
AU  - Milojević, Lazar
AU  - Velebit, Branko
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1647
AB  - The aim of this study was to assess the expression of a toxic shock syndrome toxin-1 (TSST-1) gene of Staphylococcus aureus in different types of milk, depending on inadequate temperature and storage conditions. Pasteurized and UHT milk were inoculated with monotoxic TSST-1 strain of S. aureus and growth kinetics was determined by the drop plate method using Baird-Parker agar medium in accordance with EN ISO 6888-1. The patterns of gene regulation were detected by quantitative reverse transcriptase PCR. Relative quantification method for statistical significance testing was used to detect significant gene expression responses. The results of experiments showed the dependence of the growth rate and consequent up-regulation of TSST-1 encoding gene on storage time-temperature and type of milk. In contaminated pasteurized and UHT milk kept at both 15 degrees C and 22 degrees C S. aureus significantly increased its virulent potential over time. This effect was a bit more emphasized in UHT milk serving as a proof of concept. Possible explanation could be a presence of lactic acid bacteria in pasteurized milk which is known to have down-regulatory effect on TSST-1 gene. Maintenance of the milk storage temperature below 8 degrees C and employment of microbiological control measures in hygienic practices, from milk producer through retailer and on to the consumer is of utmost importance to decrease risk of non-emetic staphylococcal poisoning.
PB  - Croatian Dairy Union, Zagreb
T2  - Mljekarstvo
T1  - Expression of toxic shock syndrome toxin-1 gene of Staphylococcus aureus in milk: Proof of concept
VL  - 68
IS  - 1
SP  - 12
EP  - 20
DO  - 10.15567/mljekarstvo.2018.0102
ER  - 
@article{
author = "Babić, Milijana and Pajić, Marija and Nikolić, Aleksandra and Teodorović, Vlado and Mirilović, Milorad and Milojević, Lazar and Velebit, Branko",
year = "2018",
abstract = "The aim of this study was to assess the expression of a toxic shock syndrome toxin-1 (TSST-1) gene of Staphylococcus aureus in different types of milk, depending on inadequate temperature and storage conditions. Pasteurized and UHT milk were inoculated with monotoxic TSST-1 strain of S. aureus and growth kinetics was determined by the drop plate method using Baird-Parker agar medium in accordance with EN ISO 6888-1. The patterns of gene regulation were detected by quantitative reverse transcriptase PCR. Relative quantification method for statistical significance testing was used to detect significant gene expression responses. The results of experiments showed the dependence of the growth rate and consequent up-regulation of TSST-1 encoding gene on storage time-temperature and type of milk. In contaminated pasteurized and UHT milk kept at both 15 degrees C and 22 degrees C S. aureus significantly increased its virulent potential over time. This effect was a bit more emphasized in UHT milk serving as a proof of concept. Possible explanation could be a presence of lactic acid bacteria in pasteurized milk which is known to have down-regulatory effect on TSST-1 gene. Maintenance of the milk storage temperature below 8 degrees C and employment of microbiological control measures in hygienic practices, from milk producer through retailer and on to the consumer is of utmost importance to decrease risk of non-emetic staphylococcal poisoning.",
publisher = "Croatian Dairy Union, Zagreb",
journal = "Mljekarstvo",
title = "Expression of toxic shock syndrome toxin-1 gene of Staphylococcus aureus in milk: Proof of concept",
volume = "68",
number = "1",
pages = "12-20",
doi = "10.15567/mljekarstvo.2018.0102"
}
Babić, M., Pajić, M., Nikolić, A., Teodorović, V., Mirilović, M., Milojević, L.,& Velebit, B.. (2018). Expression of toxic shock syndrome toxin-1 gene of Staphylococcus aureus in milk: Proof of concept. in Mljekarstvo
Croatian Dairy Union, Zagreb., 68(1), 12-20.
https://doi.org/10.15567/mljekarstvo.2018.0102
Babić M, Pajić M, Nikolić A, Teodorović V, Mirilović M, Milojević L, Velebit B. Expression of toxic shock syndrome toxin-1 gene of Staphylococcus aureus in milk: Proof of concept. in Mljekarstvo. 2018;68(1):12-20.
doi:10.15567/mljekarstvo.2018.0102 .
Babić, Milijana, Pajić, Marija, Nikolić, Aleksandra, Teodorović, Vlado, Mirilović, Milorad, Milojević, Lazar, Velebit, Branko, "Expression of toxic shock syndrome toxin-1 gene of Staphylococcus aureus in milk: Proof of concept" in Mljekarstvo, 68, no. 1 (2018):12-20,
https://doi.org/10.15567/mljekarstvo.2018.0102 . .
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Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows

Pajić, Marija; Boboš, Stanko; Velebit, Branko; Rašić, Zoran; Katić, Vera; Radinović, Miodrag; Nikolić, Aleksandra; Simonović, Dušan; Babić, Milijana

(Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd, 2016)

TY  - JOUR
AU  - Pajić, Marija
AU  - Boboš, Stanko
AU  - Velebit, Branko
AU  - Rašić, Zoran
AU  - Katić, Vera
AU  - Radinović, Miodrag
AU  - Nikolić, Aleksandra
AU  - Simonović, Dušan
AU  - Babić, Milijana
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1355
AB  - Staphylococcus aureus is known worldwide as a frequent cause of mastitis in dairy cattle. Due to the production of heath resistant enterotoxins, this pathogen is also a major cause of food poisoning among humans, with symptoms of often severe vomiting and diarrhea. The aim of our study was to determine the prevalence of enterotoxinproducing strains of S. aureus originating from samples of cows with subclinical and clinical mastitis in the Republic of Serbia. Furthermore, we analyzed the type of staphylococcal enterotoxin they produce and phylogenetic relatedness among the S. aureus isolates recovered from milk in this study. Production of staphylococcal enterotoxins A, B, C, D and E was determined by commercial immunoenzyme assay VIDAS® SET2, and presence of corresponding genes encoding enterotoxin synthesis in positive isolates confirmed by Polymerase Chain Reaction. Enterotoxin production was determined in 5 out of 75 (6.67%) isolates of S. aureus and all of them produced staphylococcal enterotoxins C. After analyzing the nucleotide sequence of the gene encoding the synthesis of staphylococcal protein A, S. aureus isolates were assigned into 2 phylogenetic groups, including 7 clusters. All S. aureus isolates with the presence of sec gene formed one cluster even dough they originated from milk samples from different farms.
AB  - Širom sveta S. aureus poznat je kao čest uzročnik mastitisa krava. Takođe predstavlja i glavni uzrok trovanja hranom nakon konzumiranja hrane kontaminirane njegovim enterotoksinima. Cilj ovog istraživanja bio je da se ispita prevalencija enterotoksinprodukujućih sojeva S. aureus poreklom iz vimena krava sa subkliničkim i kliničkim mastitisom u Srbiji, da se odredi tip enterotoksina koji produkuju i da se odredi filogenetska srodnost ovih izolata. Za određivanje sposobnosti sinteze stafilokoknih enterotoksina A, B, C, D i E korišćen je VIDAS® SET2 immunoenzimski test i utvrđeno je da 5 od 75 (6,67%) izolata S. aureus sintetiše enterotoksine. Lančanom reakcijom polimeraze na prisustvo odgovarajućih gena, utvrđeno je da svih 5 izolata poseduju gen za sintezu enterotoksina C. Analizom nukleotidnih sekvenci gena za sintezu stafilokoknog proteina A, izolati S. aureus grupisani su filogenetski u 2 grupe, odnosno u 7 klastera. Svi izolati S. aureus kod kojih je dokazano prisustvo gena za sintezu enterotoksina spadaju u isti klaster.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows
T1  - Prevalencija i molekularna karakterizacija enterotoksin-produkujućih sojeva S. aureus izolovanih iz vimena krava u Srbiji
VL  - 66
IS  - 4
SP  - 466
EP  - 477
DO  - 10.1515/acve-2016-0040
ER  - 
@article{
author = "Pajić, Marija and Boboš, Stanko and Velebit, Branko and Rašić, Zoran and Katić, Vera and Radinović, Miodrag and Nikolić, Aleksandra and Simonović, Dušan and Babić, Milijana",
year = "2016",
abstract = "Staphylococcus aureus is known worldwide as a frequent cause of mastitis in dairy cattle. Due to the production of heath resistant enterotoxins, this pathogen is also a major cause of food poisoning among humans, with symptoms of often severe vomiting and diarrhea. The aim of our study was to determine the prevalence of enterotoxinproducing strains of S. aureus originating from samples of cows with subclinical and clinical mastitis in the Republic of Serbia. Furthermore, we analyzed the type of staphylococcal enterotoxin they produce and phylogenetic relatedness among the S. aureus isolates recovered from milk in this study. Production of staphylococcal enterotoxins A, B, C, D and E was determined by commercial immunoenzyme assay VIDAS® SET2, and presence of corresponding genes encoding enterotoxin synthesis in positive isolates confirmed by Polymerase Chain Reaction. Enterotoxin production was determined in 5 out of 75 (6.67%) isolates of S. aureus and all of them produced staphylococcal enterotoxins C. After analyzing the nucleotide sequence of the gene encoding the synthesis of staphylococcal protein A, S. aureus isolates were assigned into 2 phylogenetic groups, including 7 clusters. All S. aureus isolates with the presence of sec gene formed one cluster even dough they originated from milk samples from different farms., Širom sveta S. aureus poznat je kao čest uzročnik mastitisa krava. Takođe predstavlja i glavni uzrok trovanja hranom nakon konzumiranja hrane kontaminirane njegovim enterotoksinima. Cilj ovog istraživanja bio je da se ispita prevalencija enterotoksinprodukujućih sojeva S. aureus poreklom iz vimena krava sa subkliničkim i kliničkim mastitisom u Srbiji, da se odredi tip enterotoksina koji produkuju i da se odredi filogenetska srodnost ovih izolata. Za određivanje sposobnosti sinteze stafilokoknih enterotoksina A, B, C, D i E korišćen je VIDAS® SET2 immunoenzimski test i utvrđeno je da 5 od 75 (6,67%) izolata S. aureus sintetiše enterotoksine. Lančanom reakcijom polimeraze na prisustvo odgovarajućih gena, utvrđeno je da svih 5 izolata poseduju gen za sintezu enterotoksina C. Analizom nukleotidnih sekvenci gena za sintezu stafilokoknog proteina A, izolati S. aureus grupisani su filogenetski u 2 grupe, odnosno u 7 klastera. Svi izolati S. aureus kod kojih je dokazano prisustvo gena za sintezu enterotoksina spadaju u isti klaster.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows, Prevalencija i molekularna karakterizacija enterotoksin-produkujućih sojeva S. aureus izolovanih iz vimena krava u Srbiji",
volume = "66",
number = "4",
pages = "466-477",
doi = "10.1515/acve-2016-0040"
}
Pajić, M., Boboš, S., Velebit, B., Rašić, Z., Katić, V., Radinović, M., Nikolić, A., Simonović, D.,& Babić, M.. (2016). Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(4), 466-477.
https://doi.org/10.1515/acve-2016-0040
Pajić M, Boboš S, Velebit B, Rašić Z, Katić V, Radinović M, Nikolić A, Simonović D, Babić M. Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows. in Acta Veterinaria-Beograd. 2016;66(4):466-477.
doi:10.1515/acve-2016-0040 .
Pajić, Marija, Boboš, Stanko, Velebit, Branko, Rašić, Zoran, Katić, Vera, Radinović, Miodrag, Nikolić, Aleksandra, Simonović, Dušan, Babić, Milijana, "Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows" in Acta Veterinaria-Beograd, 66, no. 4 (2016):466-477,
https://doi.org/10.1515/acve-2016-0040 . .
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