TY  - JOUR
AU  - Prodanov-Radulović, Jasna
AU  - Mirčeta, Jovan
AU  - Djurdjević, Biljana
AU  - Lazić, Sava
AU  - Aleksić-Kovačević, Sanja
AU  - Petrović, Jelena
AU  - Polaček, Vladimir
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2941
AB  - African swine fever (ASF) has been detected in many European countries since its introduction in Georgia in 2007. Serbia suffered its first case of ASF in the domestic pig population in 2019. At
the beginning of 2020, ASF was detected in wild boars in open hunting grounds in the southeastern
region of the country in districts along the country’s borders with Romania and Bulgaria. Since
then, all ASF outbreaks in wild boar were clustered in the population located in the same bordering
areas. Despite the newly implemented biosecurity protocols for hunters in 2019, ASF was detected
for the first time in June 2021 in the wild boar population located in an enclosed hunting ground
in the northeast region of the country. In this study, we reported the first ASF outbreak in a wild
boar population located in an enclosed hunting ground in close proximity to the Serbian–Romanian
border. The epizootiological data on the field investigation of the ASF outbreak, with descriptions of
the clinical signs and gross pathological lesions detected, including the total number as well as the
estimated age, sex, and postmortem interval, were analyzed. Clinical signs were detected only in
nine diseased wild boars, while in total, 149 carcasses were found in the open and enclosed part of
the hunting ground. In addition, 99 carcasses from which samples (parts of spleen or long bones)
were collected for molecular diagnostics (RT-PCR) were confirmed as ASF-positive. The results of
the epidemiological investigations indicate the central role of wild boar movements as well as the
constant risk of human-related activities in the countries bordering area.
PB  - MDPI
T2  - Pathogens
T1  - African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia
VL  - 12
IS  - 5
SP  - 691
DO  - 10.3390/pathogens12050691
ER  - 

@article{
author = "Prodanov-Radulović, Jasna and Mirčeta, Jovan and Djurdjević, Biljana and Lazić, Sava and Aleksić-Kovačević, Sanja and Petrović, Jelena and Polaček, Vladimir",
year = "2023",
abstract = "African swine fever (ASF) has been detected in many European countries since its introduction in Georgia in 2007. Serbia suffered its first case of ASF in the domestic pig population in 2019. At
the beginning of 2020, ASF was detected in wild boars in open hunting grounds in the southeastern
region of the country in districts along the country’s borders with Romania and Bulgaria. Since
then, all ASF outbreaks in wild boar were clustered in the population located in the same bordering
areas. Despite the newly implemented biosecurity protocols for hunters in 2019, ASF was detected
for the first time in June 2021 in the wild boar population located in an enclosed hunting ground
in the northeast region of the country. In this study, we reported the first ASF outbreak in a wild
boar population located in an enclosed hunting ground in close proximity to the Serbian–Romanian
border. The epizootiological data on the field investigation of the ASF outbreak, with descriptions of
the clinical signs and gross pathological lesions detected, including the total number as well as the
estimated age, sex, and postmortem interval, were analyzed. Clinical signs were detected only in
nine diseased wild boars, while in total, 149 carcasses were found in the open and enclosed part of
the hunting ground. In addition, 99 carcasses from which samples (parts of spleen or long bones)
were collected for molecular diagnostics (RT-PCR) were confirmed as ASF-positive. The results of
the epidemiological investigations indicate the central role of wild boar movements as well as the
constant risk of human-related activities in the countries bordering area.",
publisher = "MDPI",
journal = "Pathogens",
title = "African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia",
volume = "12",
number = "5",
pages = "691",
doi = "10.3390/pathogens12050691"
}

Prodanov-Radulović, J., Mirčeta, J., Djurdjević, B., Lazić, S., Aleksić-Kovačević, S., Petrović, J.,& Polaček, V.. (2023). African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia. in Pathogens
MDPI., 12(5), 691.
https://doi.org/10.3390/pathogens12050691

Prodanov-Radulović J, Mirčeta J, Djurdjević B, Lazić S, Aleksić-Kovačević S, Petrović J, Polaček V. African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia. in Pathogens. 2023;12(5):691.
doi:10.3390/pathogens12050691 .

Prodanov-Radulović, Jasna, Mirčeta, Jovan, Djurdjević, Biljana, Lazić, Sava, Aleksić-Kovačević, Sanja, Petrović, Jelena, Polaček, Vladimir, "African Swine Fever Outbreak in an Enclosed Wild Boar Hunting Ground in Serbia" in Pathogens, 12, no. 5 (2023):691,
https://doi.org/10.3390/pathogens12050691 . .

TY  - JOUR
AU  - Đurđević, Biljana
AU  - Polaček, Vladimir
AU  - Pajić, Marko
AU  - Petrović, Tamaš
AU  - Vučićević, Ivana
AU  - Vidanović, Dejan
AU  - Aleksić-Kovačević, Sanja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2706
AB  - In winter 2016/2017, the highly pathogenic avian influenza virus H5N8 was detected in
backyard poultry in Serbia for the first time. The second HPAI outbreak case in backyard poultry
was reported in 2022, caused by subtype H5N1. This is the first study that documents the laboratory
identification and pathology associated with highly pathogenic avian influenza in poultry in Serbia
during the first and second introduction waves. In both cases, the diagnosis was based on real-time
reverse transcriptase PCR. The most common observed lesions included subepicardial hemorrhages,
congestion and hemorrhages in the lungs, and petechial hemorrhages in coelomic and epicardial adipose tissue. Histologically, the observed lesions were mostly nonpurulent encephalitis accompanied
by encephalomalacia, multifocal necrosis in the spleen, pancreas, and kidneys, pulmonary congestion,
and myocardial and pulmonary hemorrhages. In H5N8-infected chickens, immunohistochemical
examination revealed strong positive IHC staining in the brain and lungs. Following these outbreaks,
strict control measures were implemented on farms and backyard holdings to prevent the occurrence
and spread of the disease. Extensive surveillance of birds for avian influenza virus did not detect
any additional cases in poultry. These outbreaks highlight the importance of a rapid detection and
response system in order to quickly suppress outbreaks.
PB  - MDPI
T2  - Animals
T1  - Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia
VL  - 13
IS  - 4
SP  - 700
DO  - 10.3390/ani13040700
ER  - 

@article{
author = "Đurđević, Biljana and Polaček, Vladimir and Pajić, Marko and Petrović, Tamaš and Vučićević, Ivana and Vidanović, Dejan and Aleksić-Kovačević, Sanja",
year = "2023",
abstract = "In winter 2016/2017, the highly pathogenic avian influenza virus H5N8 was detected in
backyard poultry in Serbia for the first time. The second HPAI outbreak case in backyard poultry
was reported in 2022, caused by subtype H5N1. This is the first study that documents the laboratory
identification and pathology associated with highly pathogenic avian influenza in poultry in Serbia
during the first and second introduction waves. In both cases, the diagnosis was based on real-time
reverse transcriptase PCR. The most common observed lesions included subepicardial hemorrhages,
congestion and hemorrhages in the lungs, and petechial hemorrhages in coelomic and epicardial adipose tissue. Histologically, the observed lesions were mostly nonpurulent encephalitis accompanied
by encephalomalacia, multifocal necrosis in the spleen, pancreas, and kidneys, pulmonary congestion,
and myocardial and pulmonary hemorrhages. In H5N8-infected chickens, immunohistochemical
examination revealed strong positive IHC staining in the brain and lungs. Following these outbreaks,
strict control measures were implemented on farms and backyard holdings to prevent the occurrence
and spread of the disease. Extensive surveillance of birds for avian influenza virus did not detect
any additional cases in poultry. These outbreaks highlight the importance of a rapid detection and
response system in order to quickly suppress outbreaks.",
publisher = "MDPI",
journal = "Animals",
title = "Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia",
volume = "13",
number = "4",
pages = "700",
doi = "10.3390/ani13040700"
}

Đurđević, B., Polaček, V., Pajić, M., Petrović, T., Vučićević, I., Vidanović, D.,& Aleksić-Kovačević, S.. (2023). Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia. in Animals
MDPI., 13(4), 700.
https://doi.org/10.3390/ani13040700

Đurđević B, Polaček V, Pajić M, Petrović T, Vučićević I, Vidanović D, Aleksić-Kovačević S. Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia. in Animals. 2023;13(4):700.
doi:10.3390/ani13040700 .

Đurđević, Biljana, Polaček, Vladimir, Pajić, Marko, Petrović, Tamaš, Vučićević, Ivana, Vidanović, Dejan, Aleksić-Kovačević, Sanja, "Highly Pathogenic Avian Influenza H5N8 Outbreak in Backyard Chickens in Serbia" in Animals, 13, no. 4 (2023):700,
https://doi.org/10.3390/ani13040700 . .

TY  - JOUR
AU  - Đurđević, Biljana
AU  - Vučićević, Ivana
AU  - Bogunović, Danica
AU  - Pajić, Marko
AU  - Vejnović, Branislav
AU  - Prodanov-Radulović, Jasna
AU  - Polaček, Vladimir
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2786
AB  - Background The Sarconema eurycerca heart infection may be one of the main causes of death in swans and geese due to
severe heart lesions. To date, there have been no reports of Sarconema eurycerca in mute swans (Cygnus olor) in Serbia and
this parasite has never been morphologically characterized in wild birds from this region.
Methods In spring 2021, eight dead mute swans (Cygnus olor) were collected during avian influenza surveillance on the territory
of the Province of Vojvodina and submitted to Scientific Veterinary Institute “Novi Sad” to determine the cause of death.
The presence of highly pathogenic avian influenza subtype H5N1 was determined in all swans using molecular methods.
Results Necropsy of the swans revealed changes characteristic for highly pathogenic avian influenza infection, such as severe
haemorrhages and necrosis in different organs. Three of the eight swans showed massive subepicardial haemorrhages and
myocarditis with intralesional 2–4 cm long whitish roundworms. Histopathologically, haemorrhages and many adult heart
worms were observed in the myocardium. Based on morphological features of parasites, infection by Sarconema eurycerca
was concluded.
Conclusion According to the available scientific literature, the presence of heart roundworms in swans has not yet been
confirmed in our country. This report is the first published case of cardiac filariasis associated with S. eurycerca infection
in mute swans in the Republic of Serbia.
PB  - Springer
T2  - Acta Parasitologica
T1  - First Report of Sarconema eurycerca Heart Infection in Mute Swans in Serbia
VL  - 67
IS  - 4
SP  - 1773
EP  - 1777
DO  - 10.1007/s11686-022-00616-3
ER  - 

@article{
author = "Đurđević, Biljana and Vučićević, Ivana and Bogunović, Danica and Pajić, Marko and Vejnović, Branislav and Prodanov-Radulović, Jasna and Polaček, Vladimir",
year = "2022",
abstract = "Background The Sarconema eurycerca heart infection may be one of the main causes of death in swans and geese due to
severe heart lesions. To date, there have been no reports of Sarconema eurycerca in mute swans (Cygnus olor) in Serbia and
this parasite has never been morphologically characterized in wild birds from this region.
Methods In spring 2021, eight dead mute swans (Cygnus olor) were collected during avian influenza surveillance on the territory
of the Province of Vojvodina and submitted to Scientific Veterinary Institute “Novi Sad” to determine the cause of death.
The presence of highly pathogenic avian influenza subtype H5N1 was determined in all swans using molecular methods.
Results Necropsy of the swans revealed changes characteristic for highly pathogenic avian influenza infection, such as severe
haemorrhages and necrosis in different organs. Three of the eight swans showed massive subepicardial haemorrhages and
myocarditis with intralesional 2–4 cm long whitish roundworms. Histopathologically, haemorrhages and many adult heart
worms were observed in the myocardium. Based on morphological features of parasites, infection by Sarconema eurycerca
was concluded.
Conclusion According to the available scientific literature, the presence of heart roundworms in swans has not yet been
confirmed in our country. This report is the first published case of cardiac filariasis associated with S. eurycerca infection
in mute swans in the Republic of Serbia.",
publisher = "Springer",
journal = "Acta Parasitologica",
title = "First Report of Sarconema eurycerca Heart Infection in Mute Swans in Serbia",
volume = "67",
number = "4",
pages = "1773-1777",
doi = "10.1007/s11686-022-00616-3"
}

Đurđević, B., Vučićević, I., Bogunović, D., Pajić, M., Vejnović, B., Prodanov-Radulović, J.,& Polaček, V.. (2022). First Report of Sarconema eurycerca Heart Infection in Mute Swans in Serbia. in Acta Parasitologica
Springer., 67(4), 1773-1777.
https://doi.org/10.1007/s11686-022-00616-3

Đurđević B, Vučićević I, Bogunović D, Pajić M, Vejnović B, Prodanov-Radulović J, Polaček V. First Report of Sarconema eurycerca Heart Infection in Mute Swans in Serbia. in Acta Parasitologica. 2022;67(4):1773-1777.
doi:10.1007/s11686-022-00616-3 .

Đurđević, Biljana, Vučićević, Ivana, Bogunović, Danica, Pajić, Marko, Vejnović, Branislav, Prodanov-Radulović, Jasna, Polaček, Vladimir, "First Report of Sarconema eurycerca Heart Infection in Mute Swans in Serbia" in Acta Parasitologica, 67, no. 4 (2022):1773-1777,
https://doi.org/10.1007/s11686-022-00616-3 . .

TY  - CONF
AU  - Vučićević, Ivana
AU  - Đurđević, Biljana
AU  - Nešić, Slađan
AU  - Aleksić-Kovačević, Sanja
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2005
AB  - Usled jesenje migracije ptica ka teritoriji Evrope, postoji kontinui- rani rizik od pojave novih slučajeva visoko patogene avijarne influence (HPAI), kako kod divljih, tako i kod domaćih ptica. Tokom jeseni 2020. godine dijagnostikovano je preko 300 slučajeva HPAI-A(H5) kod divljih ptica i živine u zemljama EU. Broj žarišta HPAI se drastično povećava i dok je proteklih godina glavni uzročnik epidemije bio A(H5N8) podtip virusa, sada je zastupljeno nekoliko podtipova: A(H5N8), A(H5N5) i A(H5N1). Kod infekcije HPAI virusima, tip promena koje se sreću na obdukciji, kao i njihova lokalizacija mogu se donekle razlikovati u zavi- snosti od podtipa virusa i vrste ptica. Promene se javljaju u vidu nekro- za, edema i hemoragija, a najčešće su locirane na plućima, srcu, jetri, slezini, bubrezima, mozgu i koži. Obdukcija ptica sumnjivih na HPAI se obavlja u prostorijama sa biosigurnosnim nivoom 2, noseći, pored standardne zaštitne opreme još i maske N95. Obdukcija se vrši prema standardnim protokolima za obdukciju ptica koji uključuju spoljašnji i unutrašnji pregled leša. Prilikom obdukcije, potrebno je uzeti uzorke tkiva promenjenih organa i ona, koja su namenjena za patohistološ- ku analizu, fiksirati u 10% puferizovanom formalinu najmanje 48 ča- sova. Uzorci ne smeju biti deblji od 0,5 cm, kako bi fiksativ magao da prodre kroz tkivo. Obdukovane leševe ptica treba neškodljivo ukloniti, kao materijal kategorije 1.
PB  - Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021
T1  - Patomorfološke promene kod avijarne influence domaćih i divljih ptica – tehnike obdukcije ptica; pravilno uzorkovanje i slanje materijala za dijagnostiku
SP  - 149
EP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2005
ER  - 

@conference{
author = "Vučićević, Ivana and Đurđević, Biljana and Nešić, Slađan and Aleksić-Kovačević, Sanja",
year = "2021",
abstract = "Usled jesenje migracije ptica ka teritoriji Evrope, postoji kontinui- rani rizik od pojave novih slučajeva visoko patogene avijarne influence (HPAI), kako kod divljih, tako i kod domaćih ptica. Tokom jeseni 2020. godine dijagnostikovano je preko 300 slučajeva HPAI-A(H5) kod divljih ptica i živine u zemljama EU. Broj žarišta HPAI se drastično povećava i dok je proteklih godina glavni uzročnik epidemije bio A(H5N8) podtip virusa, sada je zastupljeno nekoliko podtipova: A(H5N8), A(H5N5) i A(H5N1). Kod infekcije HPAI virusima, tip promena koje se sreću na obdukciji, kao i njihova lokalizacija mogu se donekle razlikovati u zavi- snosti od podtipa virusa i vrste ptica. Promene se javljaju u vidu nekro- za, edema i hemoragija, a najčešće su locirane na plućima, srcu, jetri, slezini, bubrezima, mozgu i koži. Obdukcija ptica sumnjivih na HPAI se obavlja u prostorijama sa biosigurnosnim nivoom 2, noseći, pored standardne zaštitne opreme još i maske N95. Obdukcija se vrši prema standardnim protokolima za obdukciju ptica koji uključuju spoljašnji i unutrašnji pregled leša. Prilikom obdukcije, potrebno je uzeti uzorke tkiva promenjenih organa i ona, koja su namenjena za patohistološ- ku analizu, fiksirati u 10% puferizovanom formalinu najmanje 48 ča- sova. Uzorci ne smeju biti deblji od 0,5 cm, kako bi fiksativ magao da prodre kroz tkivo. Obdukovane leševe ptica treba neškodljivo ukloniti, kao materijal kategorije 1.",
publisher = "Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021",
title = "Patomorfološke promene kod avijarne influence domaćih i divljih ptica – tehnike obdukcije ptica; pravilno uzorkovanje i slanje materijala za dijagnostiku",
pages = "149-158",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2005"
}

Vučićević, I., Đurđević, B., Nešić, S.,& Aleksić-Kovačević, S.. (2021). Patomorfološke promene kod avijarne influence domaćih i divljih ptica – tehnike obdukcije ptica; pravilno uzorkovanje i slanje materijala za dijagnostiku. in XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021
Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 149-158.
https://hdl.handle.net/21.15107/rcub_veterinar_2005

Vučićević I, Đurđević B, Nešić S, Aleksić-Kovačević S. Patomorfološke promene kod avijarne influence domaćih i divljih ptica – tehnike obdukcije ptica; pravilno uzorkovanje i slanje materijala za dijagnostiku. in XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021. 2021;:149-158.
https://hdl.handle.net/21.15107/rcub_veterinar_2005 .

Vučićević, Ivana, Đurđević, Biljana, Nešić, Slađan, Aleksić-Kovačević, Sanja, "Patomorfološke promene kod avijarne influence domaćih i divljih ptica – tehnike obdukcije ptica; pravilno uzorkovanje i slanje materijala za dijagnostiku" in XLII seminar za inovacije znanja veterinara, Beograd, 18 - 19. februar, 2021 (2021):149-158,
https://hdl.handle.net/21.15107/rcub_veterinar_2005 .

TY  - JOUR
AU  - Polaček, Vladimir
AU  - Đurđević, Biljana
AU  - Petrović, Tamaš
AU  - Prodanov-Radulović, Jasna
AU  - Samojlović, Milena
AU  - Vučićević, Ivana
AU  - Aleksić-Kovačević, Sanja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1892
AB  - The classical swine fever virus has the ability to cross the placental barrier, resulting in the infection of fetuses, which may consequently lead to persistent infection in piglets. The aim of this study was to report the lesions in fetuses naturally infected with CSFV during late gestation and clarify the nature of infected cells and the distribution of viral antigen in different tissues. A total of twenty-nine fetuses aged 82, 83 and 95 gestational days originating from three naturally CSFV infected sows were examined in this study. In all tested sows and their fetuses CSFV was detected using RT-PCR method. Immunohistochemistry method was used to detect viral antigen and monoclonal antibody WH303 was used on formalin fixed tissue samples of brain, spleen, heart, tonsils, kidney, ileocecal valve and umbilical cord. The most common lesions in the majority of fetuses were hyperemia, petechial haemorrhages in the skin, lymph nodes and kidneys. With the exception of myocardium, CSF viral antigen was detected in all the examined tissues. WH303 positive cells included endothelial cells, monocytes, macrophages and lymphocytes. The largest number of positive cells was found in kidneys in all of the examined fetuses. Reticular cells, macrophages, lymphocytes and endothelial cells in the spleen were also intensely and widely stained in most of the fetuses. These results showed that CSFV antigen can be detected in formalin-fixed, paraffin-embedded fetal tissue specimens originating from naturally CSFV infected sows by using monoclonal antibody WH303. Fetal kidneys proved to be a very useful organ for diagnosis of the CSF virus. Having that in mind, it is assumed that persistently infected piglets may shed a high amount of viral particles through urine. However, further research is needed to confirm this hypothesis.
AB  - Virus klasične kuge svinja poseduje mogućnost prelaska placentarne barijere, što može dovesti do infekcije fetusa i posledično do nastanka perzistentne infekcije kod prasadi. Cilj ovog istraživanja bio je utvrđivanje lezija koje nastaju kod fetusa prirodno inficiranih virusom klasične kuge svinja tokom  kasne  faze  gestacije, kao  i  prirodu  inficiranih  ćelija  i distribuciju virusnog antigena u različitim tkivima fetusa. Ukupno je ispitano  dvadesetdevet fetusa starosti 82, 83 i 95 dana gestacije, poreklom od tri prirodno inficirane krmače virusom klasične kuge svinja. Prisustvo virusa potvrđeno je kod svih ispitanih krmača i njihovih fetusa upotrebom RT-PCR metode. Za  imunohistohemijsku  detekciju  virusnog antigena  u  tkivnim  isečcima  mozga, slezine, srca, tonzila, bubrega, ileoceklane  valvule i pupčane vrpce primenjeno je monoklonko antitelo WH303. Kod većine  ispitanih  fetusa  ustanovljena je hiperemija i petehijlna krvavljenja na koži, limfnim čvorovima  i  bubrezima. Virusni antigen  je  detektovan  u  svim  ispitanim tkivima  fetusa, izuzev  tkiva srca. Detektovane WH303 pozitivne ćelije obuhvatale su endotelne ćelije, monocite, makrofage i limfocite. Najveći procenat  pozitivnih ćelija na virusni antigen utvrđen je u bubrezima  kod svih ispitanih  fetusa. Pored toga, veliki broj pozitivnih ćelija dokazan je u retikularnim, limfoidnim i endotelnim ćelijama slezine kod većine fetusa. Rezultati dobijeni u ovom istraživanju pokazuju da se upotrebom monoklonskog antitela WH303 može  detektovati antigen virusa klasične kuge svinja u parafinskim isečcima  tkiva  fetusa  prasadi poreklom  od  prirodno inficiranih  krmača. Pored toga, utvrđeno je da su fetalni bubrezi veoma pogodan materijal za dijagnostiku virusa klasične kuge svinja. Na osnovu ovih nalaza postavljena je hipoteza da perzistentno inficirana prasad mogu izlučivati velike količine virusnih čestica putem urina, međutim, potrebna su dodatna istraživanja kako bi se potvrdila ova hipoteza.
PB  - Scientific Veterinary Institute “Novi Sad”
T2  - Archives of Veterinary Medicine
T1  - Classical swine fever virus detection in fetal swine tissues by immunohistochemistry
T1  - Detekcija virusa klasične kuge svinja u fetalnim tkivima prasadi primenom imunohistohemijske metode
VL  - 13
IS  - 1
DO  - 10.46784/e-avm.v13i1.235
ER  - 

@article{
author = "Polaček, Vladimir and Đurđević, Biljana and Petrović, Tamaš and Prodanov-Radulović, Jasna and Samojlović, Milena and Vučićević, Ivana and Aleksić-Kovačević, Sanja",
year = "2020",
abstract = "The classical swine fever virus has the ability to cross the placental barrier, resulting in the infection of fetuses, which may consequently lead to persistent infection in piglets. The aim of this study was to report the lesions in fetuses naturally infected with CSFV during late gestation and clarify the nature of infected cells and the distribution of viral antigen in different tissues. A total of twenty-nine fetuses aged 82, 83 and 95 gestational days originating from three naturally CSFV infected sows were examined in this study. In all tested sows and their fetuses CSFV was detected using RT-PCR method. Immunohistochemistry method was used to detect viral antigen and monoclonal antibody WH303 was used on formalin fixed tissue samples of brain, spleen, heart, tonsils, kidney, ileocecal valve and umbilical cord. The most common lesions in the majority of fetuses were hyperemia, petechial haemorrhages in the skin, lymph nodes and kidneys. With the exception of myocardium, CSF viral antigen was detected in all the examined tissues. WH303 positive cells included endothelial cells, monocytes, macrophages and lymphocytes. The largest number of positive cells was found in kidneys in all of the examined fetuses. Reticular cells, macrophages, lymphocytes and endothelial cells in the spleen were also intensely and widely stained in most of the fetuses. These results showed that CSFV antigen can be detected in formalin-fixed, paraffin-embedded fetal tissue specimens originating from naturally CSFV infected sows by using monoclonal antibody WH303. Fetal kidneys proved to be a very useful organ for diagnosis of the CSF virus. Having that in mind, it is assumed that persistently infected piglets may shed a high amount of viral particles through urine. However, further research is needed to confirm this hypothesis., Virus klasične kuge svinja poseduje mogućnost prelaska placentarne barijere, što može dovesti do infekcije fetusa i posledično do nastanka perzistentne infekcije kod prasadi. Cilj ovog istraživanja bio je utvrđivanje lezija koje nastaju kod fetusa prirodno inficiranih virusom klasične kuge svinja tokom  kasne  faze  gestacije, kao  i  prirodu  inficiranih  ćelija  i distribuciju virusnog antigena u različitim tkivima fetusa. Ukupno je ispitano  dvadesetdevet fetusa starosti 82, 83 i 95 dana gestacije, poreklom od tri prirodno inficirane krmače virusom klasične kuge svinja. Prisustvo virusa potvrđeno je kod svih ispitanih krmača i njihovih fetusa upotrebom RT-PCR metode. Za  imunohistohemijsku  detekciju  virusnog antigena  u  tkivnim  isečcima  mozga, slezine, srca, tonzila, bubrega, ileoceklane  valvule i pupčane vrpce primenjeno je monoklonko antitelo WH303. Kod većine  ispitanih  fetusa  ustanovljena je hiperemija i petehijlna krvavljenja na koži, limfnim čvorovima  i  bubrezima. Virusni antigen  je  detektovan  u  svim  ispitanim tkivima  fetusa, izuzev  tkiva srca. Detektovane WH303 pozitivne ćelije obuhvatale su endotelne ćelije, monocite, makrofage i limfocite. Najveći procenat  pozitivnih ćelija na virusni antigen utvrđen je u bubrezima  kod svih ispitanih  fetusa. Pored toga, veliki broj pozitivnih ćelija dokazan je u retikularnim, limfoidnim i endotelnim ćelijama slezine kod većine fetusa. Rezultati dobijeni u ovom istraživanju pokazuju da se upotrebom monoklonskog antitela WH303 može  detektovati antigen virusa klasične kuge svinja u parafinskim isečcima  tkiva  fetusa  prasadi poreklom  od  prirodno inficiranih  krmača. Pored toga, utvrđeno je da su fetalni bubrezi veoma pogodan materijal za dijagnostiku virusa klasične kuge svinja. Na osnovu ovih nalaza postavljena je hipoteza da perzistentno inficirana prasad mogu izlučivati velike količine virusnih čestica putem urina, međutim, potrebna su dodatna istraživanja kako bi se potvrdila ova hipoteza.",
publisher = "Scientific Veterinary Institute “Novi Sad”",
journal = "Archives of Veterinary Medicine",
title = "Classical swine fever virus detection in fetal swine tissues by immunohistochemistry, Detekcija virusa klasične kuge svinja u fetalnim tkivima prasadi primenom imunohistohemijske metode",
volume = "13",
number = "1",
doi = "10.46784/e-avm.v13i1.235"
}

Polaček, V., Đurđević, B., Petrović, T., Prodanov-Radulović, J., Samojlović, M., Vučićević, I.,& Aleksić-Kovačević, S.. (2020). Classical swine fever virus detection in fetal swine tissues by immunohistochemistry. in Archives of Veterinary Medicine
Scientific Veterinary Institute “Novi Sad”., 13(1).
https://doi.org/10.46784/e-avm.v13i1.235

Polaček V, Đurđević B, Petrović T, Prodanov-Radulović J, Samojlović M, Vučićević I, Aleksić-Kovačević S. Classical swine fever virus detection in fetal swine tissues by immunohistochemistry. in Archives of Veterinary Medicine. 2020;13(1).
doi:10.46784/e-avm.v13i1.235 .

Polaček, Vladimir, Đurđević, Biljana, Petrović, Tamaš, Prodanov-Radulović, Jasna, Samojlović, Milena, Vučićević, Ivana, Aleksić-Kovačević, Sanja, "Classical swine fever virus detection in fetal swine tissues by immunohistochemistry" in Archives of Veterinary Medicine, 13, no. 1 (2020),
https://doi.org/10.46784/e-avm.v13i1.235 . .

TY  - CONF
AU  - Đurđević, Biljana
AU  - Pajić, Marko
AU  - Samojlović, Milena
AU  - Knežević, Slobodan
AU  - Vučićević, Ivana
AU  - Stojnić, Nikola
AU  - Polaček, Vladimir
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3748
AB  - У истраживању које је спроведено у току двогодишњег периода (2017. – 2018.)
анализиран је узрок угинућа појединих заштићених и строго заштићених врста
дивљих птица са територије Војводине. У научном институту за ветеринарство „Нови
Сад“ у поменутом периоду обдуковано је 57 дивљих птица (укупно 9 заштићених
врста). Извршен је детаљан макроскопски преглед свих лешева птица, док су у
појединим случајевима узоркована ткива ради микробиолошких, молекуларних и
токсиколошких анализа.
Већина прегледаних лешева птица била је у очуваном стању и добре кондиције.
Најдоминантније патоморфолошке промене уочене на органима гастроинтестиналног
система обухватале су хиперемију, задебљање и едем слузнице црева са крвављењем.
Бактериолошким прегледом црева утврђене су Escherichia coli и Proteus spp., док су
паразитолошким прегледом установљење ооцисте кокцидија. Код корморана је
уочена велика инфестација адултних облика нематода које су локализоване
првенствено у мукози провентрикулуса, као и у лумену црева и једњака. У вољци и
желуцу заштићених врста орлова утврђен је делимично мацериран садржај ружичасте
боје. Токсиколошком анализом суспектног садржаја констатовано је присуство
отрова карбофурана, указујући на алиментарну интоксикацију. Најчешће промене на
јетри биле су у виду перихепатитиса и фокалних некротичних поља. На органима
респиратоног система најучесталије макроскопске промене обухватале су конгестију
и хеморагије на плућима. Код две птице је утврђен оток инфраорбиталног синуса и
гнојни синузитис. Макроскопским прегледом срца код већине птица нису уочене
патолошке промене. Спорадично су забележене промене на бубрезима у виду едема
и хиперемије. Код неколико врста уочене су фрактуре костију са обимним поткожним
крвним подливима, као и налаз великих крвних угрушака у абдомену указујући на
трауматске озледе. Рањавање ватреним оружјем и налаз сачме утврђен је код три
птице, при чему су на обдукцији констатовани прекид континуитета костију и
мускулатуре.
На основу описаних патоморфолошких промена и резултата лабораторијских анализа
утврђено је да многобројни етиолошки фактори доприносе угинућу дивљих птица.
Међутим, резултати овог прелиминарног истраживања указују да је антропогени
фактор свакако један од честих узрока угинућа заштићених врста птица и с тим у
вези, потребно је предузети одређене мере како би се спречиле нелегалне активности
људи и негативан утицај на њихову популацију.
AB  - In the study conducted during the two-year period (2017 - 2018) the cause of death of
certain protected and strictly protected wild bird species from the territory of Vojvodina
was analysed. During this period, 57 wild birds were brought for post-mortem
examination (a total of 9 protected species) to Scientific veterinary institute "Novi Sad".
A detailed macroscopic examination of all birds was carried out, while in certain cases
samples were taken for microbiological, molecular and toxicological analyzes.
Most of the examined wild bird carcaces were in good preserved state and condition. The
most dominant pathomorphologic changes observed in the organs of the gastrointestinal
system included hyperemia, thickening and edema of the mucous membrane of the
intestines with haemorrhages. Escherichia coli and Proteus spp. were determined by
bacterial analysis of the intestine, while the coccidial oocysts were found by
parasitological examination. A large infestation of adult forms of nematodes was found in
cormorants, which were localized primarily in the mucosa of the proventriculus as well as
in the lumen of the intestine and esophagus. In the crop and gizzard of protected eagle
species, partially macerated content of pink color was determined. Toxicological analysis
of the susceptible content showed the presence of carbofuran poison, indicating an
alimentary intoxication. The most frequent changes in the liver were in the form of
perihepatitis and the occurrence of focal necrotic fields. The most common macroscopic
changes in organs of respiratory system included congestion and haemorrhages in the
lungs. An infraorbital sinus edema and purulent sinusitis have been identified in two birds.
Macroscopic examination of the heart showed no pathological changes in most of the
birds. Kidney changes in the form of edema and congestion were observed sporadically.
Bone fractures with extensive subcutaneous hematomas, as well as the finding of large
blood clots in the abdomen indicated traumatic injuries in several bird species. A buckshot
was found in three birds indicating wounding by firearms, and bone and muscle ruptures
were detected at the necropsy.
Based on the above-mentioned pathomorphological changes and the results of laboratory
analyzes, it can be concluded that numerous etiological factors contribute to mortality of
wild birds. However, the results of this preliminary study indicate that the anthropogenic
factor is certainly one of the common causes of death of protected wild bird species and
in this regard, certain measures need to be taken to prevent people's illegal activities and
the negative impact on wild bird population.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 -10. april 2019
T1  - Analiza patomorfoloških promena i uzroka mortaliteta pojedinih zaštićenih vrsta divljih ptica u Vojvodini
T1  - Analysis of pathomorphological changes and causes of mortality of certian protected wild bird species in Vojvodina
SP  - 162
EP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3748
ER  - 

@conference{
author = "Đurđević, Biljana and Pajić, Marko and Samojlović, Milena and Knežević, Slobodan and Vučićević, Ivana and Stojnić, Nikola and Polaček, Vladimir",
year = "2019",
abstract = "У истраживању које је спроведено у току двогодишњег периода (2017. – 2018.)
анализиран је узрок угинућа појединих заштићених и строго заштићених врста
дивљих птица са територије Војводине. У научном институту за ветеринарство „Нови
Сад“ у поменутом периоду обдуковано је 57 дивљих птица (укупно 9 заштићених
врста). Извршен је детаљан макроскопски преглед свих лешева птица, док су у
појединим случајевима узоркована ткива ради микробиолошких, молекуларних и
токсиколошких анализа.
Већина прегледаних лешева птица била је у очуваном стању и добре кондиције.
Најдоминантније патоморфолошке промене уочене на органима гастроинтестиналног
система обухватале су хиперемију, задебљање и едем слузнице црева са крвављењем.
Бактериолошким прегледом црева утврђене су Escherichia coli и Proteus spp., док су
паразитолошким прегледом установљење ооцисте кокцидија. Код корморана је
уочена велика инфестација адултних облика нематода које су локализоване
првенствено у мукози провентрикулуса, као и у лумену црева и једњака. У вољци и
желуцу заштићених врста орлова утврђен је делимично мацериран садржај ружичасте
боје. Токсиколошком анализом суспектног садржаја констатовано је присуство
отрова карбофурана, указујући на алиментарну интоксикацију. Најчешће промене на
јетри биле су у виду перихепатитиса и фокалних некротичних поља. На органима
респиратоног система најучесталије макроскопске промене обухватале су конгестију
и хеморагије на плућима. Код две птице је утврђен оток инфраорбиталног синуса и
гнојни синузитис. Макроскопским прегледом срца код већине птица нису уочене
патолошке промене. Спорадично су забележене промене на бубрезима у виду едема
и хиперемије. Код неколико врста уочене су фрактуре костију са обимним поткожним
крвним подливима, као и налаз великих крвних угрушака у абдомену указујући на
трауматске озледе. Рањавање ватреним оружјем и налаз сачме утврђен је код три
птице, при чему су на обдукцији констатовани прекид континуитета костију и
мускулатуре.
На основу описаних патоморфолошких промена и резултата лабораторијских анализа
утврђено је да многобројни етиолошки фактори доприносе угинућу дивљих птица.
Међутим, резултати овог прелиминарног истраживања указују да је антропогени
фактор свакако један од честих узрока угинућа заштићених врста птица и с тим у
вези, потребно је предузети одређене мере како би се спречиле нелегалне активности
људи и негативан утицај на њихову популацију., In the study conducted during the two-year period (2017 - 2018) the cause of death of
certain protected and strictly protected wild bird species from the territory of Vojvodina
was analysed. During this period, 57 wild birds were brought for post-mortem
examination (a total of 9 protected species) to Scientific veterinary institute "Novi Sad".
A detailed macroscopic examination of all birds was carried out, while in certain cases
samples were taken for microbiological, molecular and toxicological analyzes.
Most of the examined wild bird carcaces were in good preserved state and condition. The
most dominant pathomorphologic changes observed in the organs of the gastrointestinal
system included hyperemia, thickening and edema of the mucous membrane of the
intestines with haemorrhages. Escherichia coli and Proteus spp. were determined by
bacterial analysis of the intestine, while the coccidial oocysts were found by
parasitological examination. A large infestation of adult forms of nematodes was found in
cormorants, which were localized primarily in the mucosa of the proventriculus as well as
in the lumen of the intestine and esophagus. In the crop and gizzard of protected eagle
species, partially macerated content of pink color was determined. Toxicological analysis
of the susceptible content showed the presence of carbofuran poison, indicating an
alimentary intoxication. The most frequent changes in the liver were in the form of
perihepatitis and the occurrence of focal necrotic fields. The most common macroscopic
changes in organs of respiratory system included congestion and haemorrhages in the
lungs. An infraorbital sinus edema and purulent sinusitis have been identified in two birds.
Macroscopic examination of the heart showed no pathological changes in most of the
birds. Kidney changes in the form of edema and congestion were observed sporadically.
Bone fractures with extensive subcutaneous hematomas, as well as the finding of large
blood clots in the abdomen indicated traumatic injuries in several bird species. A buckshot
was found in three birds indicating wounding by firearms, and bone and muscle ruptures
were detected at the necropsy.
Based on the above-mentioned pathomorphological changes and the results of laboratory
analyzes, it can be concluded that numerous etiological factors contribute to mortality of
wild birds. However, the results of this preliminary study indicate that the anthropogenic
factor is certainly one of the common causes of death of protected wild bird species and
in this regard, certain measures need to be taken to prevent people's illegal activities and
the negative impact on wild bird population.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 -10. april 2019",
title = "Analiza patomorfoloških promena i uzroka mortaliteta pojedinih zaštićenih vrsta divljih ptica u Vojvodini, Analysis of pathomorphological changes and causes of mortality of certian protected wild bird species in Vojvodina",
pages = "162-163",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3748"
}

Đurđević, B., Pajić, M., Samojlović, M., Knežević, S., Vučićević, I., Stojnić, N.,& Polaček, V.. (2019). Analiza patomorfoloških promena i uzroka mortaliteta pojedinih zaštićenih vrsta divljih ptica u Vojvodini. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 -10. april 2019
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 162-163.
https://hdl.handle.net/21.15107/rcub_veterinar_3748

Đurđević B, Pajić M, Samojlović M, Knežević S, Vučićević I, Stojnić N, Polaček V. Analiza patomorfoloških promena i uzroka mortaliteta pojedinih zaštićenih vrsta divljih ptica u Vojvodini. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 -10. april 2019. 2019;:162-163.
https://hdl.handle.net/21.15107/rcub_veterinar_3748 .

Đurđević, Biljana, Pajić, Marko, Samojlović, Milena, Knežević, Slobodan, Vučićević, Ivana, Stojnić, Nikola, Polaček, Vladimir, "Analiza patomorfoloških promena i uzroka mortaliteta pojedinih zaštićenih vrsta divljih ptica u Vojvodini" in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani), Novi Sad, 8 -10. april 2019 (2019):162-163,
https://hdl.handle.net/21.15107/rcub_veterinar_3748 .