TY  - JOUR
AU  - Vasilev, S.
AU  - Suvajdžić, Branko
AU  - Mitic, I.
AU  - Sabljić, Lj.
AU  - Karabasil, Nedjeljko
AU  - Sofronić Milosavljević, Lj.
AU  - Vasilev, Dragan
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2495
AB  - Trichinellosis is an important worldwide foodborne zoonosis. The gold standard test to detect Trichinella spp. larvae in muscle samples of animals intended for human consumption is the artificial digestion method. Handling and dispensing of conventional pepsin powder present significant safety risks for analysts. The use of pepsin powder that is resistant to aerosolization should alleviate these safety concerns. The aim of this study was to compare the efficacy of an aerosol-resistant pepsin powder to conventional pepsin powder in the artificial digestion method. Proficiency samples of pork diaphragm containing specific numbers of viable Trichinella spiralis larvae were tested in two laboratories. The results revealed that aerosol-resistant pepsin was simple, effective and convenient to use, and showed good solubility and larval recovery that met the requirements of the European Union regulation EU 2015/1375. Overall, the efficacy of the aerosol-resistant pepsin was comparable to the conventional pepsin and safer for analysts.
PB  - Cambridge University Press
T2  - Journal of Helminthology
T1  - Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat
VL  - 96
SP  - e71
DO  - 10.1017/S0022149X2200061X
ER  - 

@article{
author = "Vasilev, S. and Suvajdžić, Branko and Mitic, I. and Sabljić, Lj. and Karabasil, Nedjeljko and Sofronić Milosavljević, Lj. and Vasilev, Dragan",
year = "2022",
abstract = "Trichinellosis is an important worldwide foodborne zoonosis. The gold standard test to detect Trichinella spp. larvae in muscle samples of animals intended for human consumption is the artificial digestion method. Handling and dispensing of conventional pepsin powder present significant safety risks for analysts. The use of pepsin powder that is resistant to aerosolization should alleviate these safety concerns. The aim of this study was to compare the efficacy of an aerosol-resistant pepsin powder to conventional pepsin powder in the artificial digestion method. Proficiency samples of pork diaphragm containing specific numbers of viable Trichinella spiralis larvae were tested in two laboratories. The results revealed that aerosol-resistant pepsin was simple, effective and convenient to use, and showed good solubility and larval recovery that met the requirements of the European Union regulation EU 2015/1375. Overall, the efficacy of the aerosol-resistant pepsin was comparable to the conventional pepsin and safer for analysts.",
publisher = "Cambridge University Press",
journal = "Journal of Helminthology",
title = "Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat",
volume = "96",
pages = "e71",
doi = "10.1017/S0022149X2200061X"
}

Vasilev, S., Suvajdžić, B., Mitic, I., Sabljić, Lj., Karabasil, N., Sofronić Milosavljević, Lj.,& Vasilev, D.. (2022). Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat. in Journal of Helminthology
Cambridge University Press., 96, e71.
https://doi.org/10.1017/S0022149X2200061X

Vasilev S, Suvajdžić B, Mitic I, Sabljić L, Karabasil N, Sofronić Milosavljević L, Vasilev D. Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat. in Journal of Helminthology. 2022;96:e71.
doi:10.1017/S0022149X2200061X .

Vasilev, S., Suvajdžić, Branko, Mitic, I., Sabljić, Lj., Karabasil, Nedjeljko, Sofronić Milosavljević, Lj., Vasilev, Dragan, "Efficacy of an aerosol-resistant pepsin powder used in artificial digestion for the detection of Trichinella larvae in meat" in Journal of Helminthology, 96 (2022):e71,
https://doi.org/10.1017/S0022149X2200061X . .