@article{
author = "Zelenović, Mladen and Marinković, Darko and Stević, Nataša and Stanojević, Slavoljub and Aničić, Milan and Milićević, Vesna and Valčić, Olivera and Radojičić, Sonja",
year = "2024",
abstract = "Brucella ovis infects sheep and causes a clinical or subclinical disease characterized by
genital lesions and reduced fertility in rams, placentitis and abortions in ewes, and
increased perinatal mortality in lambs. As part of this study, analyses were conducted
on a sheep farm near Belgrade (Serbia). Of the serological tests, indirect ELISA was
performed. A total of 94 blood sera were analyzed, 33 from rams and 61 from ewes.
The results showed 23 (69.7%) positive findings in rams and 2 (3.3%) positive findings
in ewes, with an overall prevalence of 25.4% bounded by a 95% confidence interval.
Bruce-ladder multiplex PCR and Real time PCR were performed on 19 serologically
positive rams and two serologically positive ewes. The results indicate a slightly
higher sensitivity of Real time PCR compared to conventional PCR in diagnosing
B. ovis from the reproductive tissues of rams. However, the differences in sensitivity
between different nucleic acid extraction protocols were not significant. Most rams
showed a positive PCR result in only one sample of reproductive tissue, suggesting the
recommendation to take multiple samples from each animal. Further research is needed
to bring the sensitivity of molecular tests in diagnosing ram epididymitis closer to the
sensitivity of serological tests.",
publisher = "Belgrade : Faculty of Veterinary Medicine",
journal = "Acta Veterinaria",
title = "Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis",
volume = "74",
number = "1",
pages = "133-144",
doi = "10.2478/acve-2024-0010"
}
