TY  - CONF
AU  - Vasilev, Saša
AU  - Mirilović, Milorad
AU  - Ignjatović, Marija
AU  - Milakara, Emina
AU  - Mitić, Ivana
AU  - Plavšić, Budimir
AU  - Plavša, Dragana
AU  - Bošković, Tamara
AU  - Vasilev, Dragan
AU  - Karabasil, Nedjeljko
AU  - Sabljić, Ljiljana
AU  - Ilić, Nataša
AU  - Živojinović, Milena
AU  - Sofronić Milosavljević, Ljiljana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2486
AB  - U Srbiji trihineloza je jedna od najznačajnijih zoonoza koje se prenose hranom. Prvi izveštaj o nalazu kod svinja je iz 1918. godine, a prvi slučaj kod ljudi 1923. godine u Zemunu. Detekcija prisustva trihinele je prvobitno vršena trihineloskopijom. Od 1984. uvedena je veštačka digestija. Savremeni sistemi proizvodnje svinjskog mesa, mere kontrole, metoda veštačke digestije eliminisali su meso svinja sa velikih farmi kao izvor trihineloze. Prvi put u Srbiji Nacionalna referentna laboratorija za trihinelozu (NRLT) organizovala je 2017. godine kontrolu kvaliteta rada za detekciju larvi trihinele u mesu metodom magnetne mešalice (MSM). Svi učesnici (veterinarski instituti) uspešno su prošli testiranje. Članovi tima NRLT napravili su (2019. godine) novi cELISA test za otkrivanje infekcije trihinelom kod životinja i ljudi i Svetska komisija za trihinelozu ga je prepoznala kao koristan za međunarodnu upotrebu. Epidemiološki podaci iz poslednjih decenija ukazuju da je broj obolelih ljudi kao i broj zaraženih životinja značajno smanjen. Svinjsko meso je godinama najčešći izvor infekcije ljudi u Srbiji. Slučajevi se javljaju u porodičnim epidemijama i rizik je povezan sa konzumacijom svinjskog mesa u kome se nalaze larve Trichinella, a uzgajaju se bez primene higijenskih pravila i životinje nisu veterinarski testirane (epidemija u Obrenovcu i Parizu, Francuska). U većini slučajeva T. spiralis je bila etiološki agens infekcije, ali smo u 2016. godini imali veliku epidemiju izazvanu konzumacijom mesa divljih svinja koje je sadržalo larve T britovi (Čajetina, 123 osobe). Važno je: 1. Lovce i potrošače mesa svinja i mesa divljači u Srbiji edukovati o riziku u vezi sa konzumiranjem neproverenog mesa. 2. Kontrola sistema kvaliteta kod veterinarskih subjekata koji rade testiranje na prisustvo larvi trihinele u uzorcima mesa, kao i redovno učešće u PT.
AB  - In Serbia Trichinellosis is one of the most important foodborne zoonotic diseases. The first report of swine infection was in 1918 and first human case was in 1923. From 1984 artificial digestion was adopted for use in preventing human trichinellosis. Modern pork production systems, implemented control measures, artificial digestion method have eliminated farm pork as a source for trichinellosis. For the first time in Serbia National Reference Laboratory for trichinellosis (NRLT) organized in 2017 proficiency test (PT) for the detection of Trichinella larvae in meat by Magnetic Stirrer Method (MSM). All participants (veterinary institutes) successfully passed the testing. Members of NRLT team made (in 2019) new cELISA test for detection of Trichinella infection in animals and humans and the ICT recognized the test as useful for international use. Epidemiological data from the last decades indicate that the number of human cases as well as the number of infected animals has decreased significantly. Over the years, pork was the most frequent source of human trichinellosis. Cases generally occurred in family outbreaks and risk is linked to untested backyard pork consumption. Meat and meat products offered to relatives and friends may be source of infection with Trichinella when backyard pigs are raised without any compliance with hygienic rules and animals are not veterinary tested. In most numbers of outbreaks T. spiralis were the etiological agent of infection but in 2016 we had a large outbreak provoked by consumption of wild boar meat containing T britovi larvae. It is important that: 1. Hunters and consumers of backyard pigs and wild game meat should be educated about the risk associated with consumption of untested meat. 2. Control of Trichinella QA system in veterinary subjects (testing for Trichinella presence in meat samples) and regularly participation in PTs are needed to achieve safe food for consumers.
PB  - Veterinarska komora Republike Srpske
C3  - 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina)
T1  - Trichinella infection in Serbia
T1  - Infekcija trihinelom u Srbiji
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2486
ER  - 

@conference{
author = "Vasilev, Saša and Mirilović, Milorad and Ignjatović, Marija and Milakara, Emina and Mitić, Ivana and Plavšić, Budimir and Plavša, Dragana and Bošković, Tamara and Vasilev, Dragan and Karabasil, Nedjeljko and Sabljić, Ljiljana and Ilić, Nataša and Živojinović, Milena and Sofronić Milosavljević, Ljiljana",
year = "2022",
abstract = "U Srbiji trihineloza je jedna od najznačajnijih zoonoza koje se prenose hranom. Prvi izveštaj o nalazu kod svinja je iz 1918. godine, a prvi slučaj kod ljudi 1923. godine u Zemunu. Detekcija prisustva trihinele je prvobitno vršena trihineloskopijom. Od 1984. uvedena je veštačka digestija. Savremeni sistemi proizvodnje svinjskog mesa, mere kontrole, metoda veštačke digestije eliminisali su meso svinja sa velikih farmi kao izvor trihineloze. Prvi put u Srbiji Nacionalna referentna laboratorija za trihinelozu (NRLT) organizovala je 2017. godine kontrolu kvaliteta rada za detekciju larvi trihinele u mesu metodom magnetne mešalice (MSM). Svi učesnici (veterinarski instituti) uspešno su prošli testiranje. Članovi tima NRLT napravili su (2019. godine) novi cELISA test za otkrivanje infekcije trihinelom kod životinja i ljudi i Svetska komisija za trihinelozu ga je prepoznala kao koristan za međunarodnu upotrebu. Epidemiološki podaci iz poslednjih decenija ukazuju da je broj obolelih ljudi kao i broj zaraženih životinja značajno smanjen. Svinjsko meso je godinama najčešći izvor infekcije ljudi u Srbiji. Slučajevi se javljaju u porodičnim epidemijama i rizik je povezan sa konzumacijom svinjskog mesa u kome se nalaze larve Trichinella, a uzgajaju se bez primene higijenskih pravila i životinje nisu veterinarski testirane (epidemija u Obrenovcu i Parizu, Francuska). U većini slučajeva T. spiralis je bila etiološki agens infekcije, ali smo u 2016. godini imali veliku epidemiju izazvanu konzumacijom mesa divljih svinja koje je sadržalo larve T britovi (Čajetina, 123 osobe). Važno je: 1. Lovce i potrošače mesa svinja i mesa divljači u Srbiji edukovati o riziku u vezi sa konzumiranjem neproverenog mesa. 2. Kontrola sistema kvaliteta kod veterinarskih subjekata koji rade testiranje na prisustvo larvi trihinele u uzorcima mesa, kao i redovno učešće u PT., In Serbia Trichinellosis is one of the most important foodborne zoonotic diseases. The first report of swine infection was in 1918 and first human case was in 1923. From 1984 artificial digestion was adopted for use in preventing human trichinellosis. Modern pork production systems, implemented control measures, artificial digestion method have eliminated farm pork as a source for trichinellosis. For the first time in Serbia National Reference Laboratory for trichinellosis (NRLT) organized in 2017 proficiency test (PT) for the detection of Trichinella larvae in meat by Magnetic Stirrer Method (MSM). All participants (veterinary institutes) successfully passed the testing. Members of NRLT team made (in 2019) new cELISA test for detection of Trichinella infection in animals and humans and the ICT recognized the test as useful for international use. Epidemiological data from the last decades indicate that the number of human cases as well as the number of infected animals has decreased significantly. Over the years, pork was the most frequent source of human trichinellosis. Cases generally occurred in family outbreaks and risk is linked to untested backyard pork consumption. Meat and meat products offered to relatives and friends may be source of infection with Trichinella when backyard pigs are raised without any compliance with hygienic rules and animals are not veterinary tested. In most numbers of outbreaks T. spiralis were the etiological agent of infection but in 2016 we had a large outbreak provoked by consumption of wild boar meat containing T britovi larvae. It is important that: 1. Hunters and consumers of backyard pigs and wild game meat should be educated about the risk associated with consumption of untested meat. 2. Control of Trichinella QA system in veterinary subjects (testing for Trichinella presence in meat samples) and regularly participation in PTs are needed to achieve safe food for consumers.",
publisher = "Veterinarska komora Republike Srpske",
journal = "27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina)",
title = "Trichinella infection in Serbia, Infekcija trihinelom u Srbiji",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2486"
}

Vasilev, S., Mirilović, M., Ignjatović, M., Milakara, E., Mitić, I., Plavšić, B., Plavša, D., Bošković, T., Vasilev, D., Karabasil, N., Sabljić, L., Ilić, N., Živojinović, M.,& Sofronić Milosavljević, L.. (2022). Trichinella infection in Serbia. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina)
Veterinarska komora Republike Srpske..
https://hdl.handle.net/21.15107/rcub_veterinar_2486

Vasilev S, Mirilović M, Ignjatović M, Milakara E, Mitić I, Plavšić B, Plavša D, Bošković T, Vasilev D, Karabasil N, Sabljić L, Ilić N, Živojinović M, Sofronić Milosavljević L. Trichinella infection in Serbia. in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina). 2022;.
https://hdl.handle.net/21.15107/rcub_veterinar_2486 .

Vasilev, Saša, Mirilović, Milorad, Ignjatović, Marija, Milakara, Emina, Mitić, Ivana, Plavšić, Budimir, Plavša, Dragana, Bošković, Tamara, Vasilev, Dragan, Karabasil, Nedjeljko, Sabljić, Ljiljana, Ilić, Nataša, Živojinović, Milena, Sofronić Milosavljević, Ljiljana, "Trichinella infection in Serbia" in 27. godišnje savjetovanje doktora veterinarske medicine Republike Srpske (Bosna i Hercegovina) (2022),
https://hdl.handle.net/21.15107/rcub_veterinar_2486 .

TY  - JOUR
AU  - Ilić, Nataša
AU  - Gruden-Movsesijan, Alisa
AU  - Živojinović, Milena
AU  - Sofronić-Milosavljević, Ljiljana
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2182
AB  - Western blot (Wb) is considered to be the gold standard test for Trichinella infection serology, since this method allows specifi c Trichinella antigens to be distinguished from cross-reactive antigens. This is not the case with widely used antibody assay techniques - indirect immunofl uorescence and ELISA - which are sensitive, but subject to crossreactions that make the interpretation of weakly positive results diffi cult. Application of Trichinella spiralis muscle larvae excretory-secretory (ES) antigens for the specifi c antibody detection in ELISA resulted in improved specifi city compared to that of crude worm extract that was previously in use, but since production of ES has not yet been standardized, differences among laboratories occur. For this reason, the Wb profi le of serum samples from different T. spiralis infected host species: human, horse, swine and dog, was investigated in the Serbian National Reference Laboratory for Trichinellosis (NRLT). The common feature of the obtained Wb profi les was the appearance of a triad of bands with molecular masses (Mw) of 45, 49, and 53 kDa. The very same triad was recognized by a monoclonal antibody (mAb) 7C2C5 specifi c for an immunodominant epitope unique to the muscle larvae stage of all species in the genus Trichinella. Inhibition studies confi rmed that mAb and anti-Trichinella antibodies from sera competed for the same parasite epitope. Based on the obtained results, the NRLT introduced the recognition of the above mention triad as the basis for specifi c anti-Trichinella antibodies detection in the sera of infected hosts.
AB  - Western blot (Wb) metoda se smatra zlatnim standardom u serološkoj dijagnostici trihineloze obzirom da ova metoda omogućava razlikovanje specifičnih Trichinella antigena od onih koji daju ukrštenu reakciju. Ovo nije slučaj sa drugim, u praksi češće korišćenim tehnikama baziranim na detekciji antitela – indirektna imunofl uorescencija i ELISA, koje su senzitivne ali podložne unakrsnoj reaktivnosti što otežava interpretaciju slabo pozitivnih rezultata. Primena ekskretorno-sekretornog (ES) antigena larvi u mišićima, Trichinella spiralis u detekciji specifičnih antitela u ELISA testu, rezultirala je povećanom specifičnošću testa u odnosu na prethodni koji je bio baziran na primeni totalnog solubilnog ekstrakta parazita. Ipak, obzirom da proizvodnja ES antigena još uvek nije standardizovana, postoje izvesne razlike između rezultata dobijenih u različitim laboratorijama. Iz ovog razloga, u Nacionalnoj Referentnoj Laboratoriji Srbije za Trihinelozu (NRLT) ispitivali smo Wb profi l uzoraka seruma različitih vrsta domaćina infi ciranih sa T. spiralis, i to ljudi, konja, svinja i pasa. Zajedničko svojstvo dobijenih Wb profi la bila je pojava tripleta traka koje odgovaraju proteinima sa molekulskim masama (Mw) od 45, 49, 53 kDa. Isti triplet prepoznaju i monoklonska antitela (mAt) 7C2C5 specifična za imunodominantni epitop karakterističan za stadijum mišićnih larvi svih vrsta parazita iz genusa Trichinella. Eksperimentalne studije inhibicije su pokazale da postoji kompeticija između mAt i anti-Trichinella antitela iz seruma za isti epitop parazita. Na osnovu dobijenih rezultata, NRLT uvodi prepoznavanje pomenutog tripleta traka kao osnovu za detekciju specifičnih anti-Trichinella antitela u serumima infi ciranih domaćina.
T2  - Acta Veterinaria-Beograd
T1  - Characteristic band pattern in western blots for specific detection of anti- antibodies in different host species
T1  - Obrazac traka u western blot analizi karakterističan za detekciju specifičnih anti-trichinella spiralis antitela kod različitih vrsta domaćina
VL  - 64
IS  - 1
SP  - 33
EP  - 43
DO  - 10.2478/acve-2014-0004
ER  - 

@article{
author = "Ilić, Nataša and Gruden-Movsesijan, Alisa and Živojinović, Milena and Sofronić-Milosavljević, Ljiljana",
year = "2014",
abstract = "Western blot (Wb) is considered to be the gold standard test for Trichinella infection serology, since this method allows specifi c Trichinella antigens to be distinguished from cross-reactive antigens. This is not the case with widely used antibody assay techniques - indirect immunofl uorescence and ELISA - which are sensitive, but subject to crossreactions that make the interpretation of weakly positive results diffi cult. Application of Trichinella spiralis muscle larvae excretory-secretory (ES) antigens for the specifi c antibody detection in ELISA resulted in improved specifi city compared to that of crude worm extract that was previously in use, but since production of ES has not yet been standardized, differences among laboratories occur. For this reason, the Wb profi le of serum samples from different T. spiralis infected host species: human, horse, swine and dog, was investigated in the Serbian National Reference Laboratory for Trichinellosis (NRLT). The common feature of the obtained Wb profi les was the appearance of a triad of bands with molecular masses (Mw) of 45, 49, and 53 kDa. The very same triad was recognized by a monoclonal antibody (mAb) 7C2C5 specifi c for an immunodominant epitope unique to the muscle larvae stage of all species in the genus Trichinella. Inhibition studies confi rmed that mAb and anti-Trichinella antibodies from sera competed for the same parasite epitope. Based on the obtained results, the NRLT introduced the recognition of the above mention triad as the basis for specifi c anti-Trichinella antibodies detection in the sera of infected hosts., Western blot (Wb) metoda se smatra zlatnim standardom u serološkoj dijagnostici trihineloze obzirom da ova metoda omogućava razlikovanje specifičnih Trichinella antigena od onih koji daju ukrštenu reakciju. Ovo nije slučaj sa drugim, u praksi češće korišćenim tehnikama baziranim na detekciji antitela – indirektna imunofl uorescencija i ELISA, koje su senzitivne ali podložne unakrsnoj reaktivnosti što otežava interpretaciju slabo pozitivnih rezultata. Primena ekskretorno-sekretornog (ES) antigena larvi u mišićima, Trichinella spiralis u detekciji specifičnih antitela u ELISA testu, rezultirala je povećanom specifičnošću testa u odnosu na prethodni koji je bio baziran na primeni totalnog solubilnog ekstrakta parazita. Ipak, obzirom da proizvodnja ES antigena još uvek nije standardizovana, postoje izvesne razlike između rezultata dobijenih u različitim laboratorijama. Iz ovog razloga, u Nacionalnoj Referentnoj Laboratoriji Srbije za Trihinelozu (NRLT) ispitivali smo Wb profi l uzoraka seruma različitih vrsta domaćina infi ciranih sa T. spiralis, i to ljudi, konja, svinja i pasa. Zajedničko svojstvo dobijenih Wb profi la bila je pojava tripleta traka koje odgovaraju proteinima sa molekulskim masama (Mw) od 45, 49, 53 kDa. Isti triplet prepoznaju i monoklonska antitela (mAt) 7C2C5 specifična za imunodominantni epitop karakterističan za stadijum mišićnih larvi svih vrsta parazita iz genusa Trichinella. Eksperimentalne studije inhibicije su pokazale da postoji kompeticija između mAt i anti-Trichinella antitela iz seruma za isti epitop parazita. Na osnovu dobijenih rezultata, NRLT uvodi prepoznavanje pomenutog tripleta traka kao osnovu za detekciju specifičnih anti-Trichinella antitela u serumima infi ciranih domaćina.",
journal = "Acta Veterinaria-Beograd",
title = "Characteristic band pattern in western blots for specific detection of anti- antibodies in different host species, Obrazac traka u western blot analizi karakterističan za detekciju specifičnih anti-trichinella spiralis antitela kod različitih vrsta domaćina",
volume = "64",
number = "1",
pages = "33-43",
doi = "10.2478/acve-2014-0004"
}

Ilić, N., Gruden-Movsesijan, A., Živojinović, M.,& Sofronić-Milosavljević, L.. (2014). Characteristic band pattern in western blots for specific detection of anti- antibodies in different host species. in Acta Veterinaria-Beograd, 64(1), 33-43.
https://doi.org/10.2478/acve-2014-0004

Ilić N, Gruden-Movsesijan A, Živojinović M, Sofronić-Milosavljević L. Characteristic band pattern in western blots for specific detection of anti- antibodies in different host species. in Acta Veterinaria-Beograd. 2014;64(1):33-43.
doi:10.2478/acve-2014-0004 .

Ilić, Nataša, Gruden-Movsesijan, Alisa, Živojinović, Milena, Sofronić-Milosavljević, Ljiljana, "Characteristic band pattern in western blots for specific detection of anti- antibodies in different host species" in Acta Veterinaria-Beograd, 64, no. 1 (2014):33-43,
https://doi.org/10.2478/acve-2014-0004 . .