TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Borozan, Sunčica
AU  - Dimitrijević-Srećković, Vesna
AU  - Pajović, Nevena
AU  - Mirilović, Milorad
AU  - Stopper, Helga
AU  - Stanimirović, Zoran
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2811
AB  - Diabetes, a chronic group of medical disorders characterized byhyperglycemia, has become a global pandemic. Some hormones may influence the course and outcome of diabetes, especially if they potentiate the formation of reactive oxygen species (ROS). There is a close relationship between thyroid disorders and diabetes. The main objective of this investigation was to find out whether peripheral blood mononuclear cells (PBMCs) are more prone to DNA damage by triiodothyronine (T3) (0.1, 1 and 10 μM) at various stages of progression through diabetes (obese, prediabetics, and type 2 diabetes mellitus—T2DM persons). In addition, some biochemical parameters of oxidative stress (catalase-CAT, thiobarbituric acid reactive substances—TBARS) and lactate dehydrogenase (LDH) were evaluated. PBMCs from prediabetic and diabetic patients exhibited increased sensitivity for T3 regarding elevated level of DNA damage, inhibition of catalase, and increase of TBARS and LDH. PBMCs from obese patients reacted in the same manner, except for DNA damage. The results of this study should contribute to a better understanding of the role of thyroid hormones in the progression of T2DM.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro
VL  - 23
VL  - 16
SP  - 9072
DO  - 10.3390/ijms23169072
ER  - 

@article{
author = "Đelić, Ninoslav and Borozan, Sunčica and Dimitrijević-Srećković, Vesna and Pajović, Nevena and Mirilović, Milorad and Stopper, Helga and Stanimirović, Zoran",
year = "2022",
abstract = "Diabetes, a chronic group of medical disorders characterized byhyperglycemia, has become a global pandemic. Some hormones may influence the course and outcome of diabetes, especially if they potentiate the formation of reactive oxygen species (ROS). There is a close relationship between thyroid disorders and diabetes. The main objective of this investigation was to find out whether peripheral blood mononuclear cells (PBMCs) are more prone to DNA damage by triiodothyronine (T3) (0.1, 1 and 10 μM) at various stages of progression through diabetes (obese, prediabetics, and type 2 diabetes mellitus—T2DM persons). In addition, some biochemical parameters of oxidative stress (catalase-CAT, thiobarbituric acid reactive substances—TBARS) and lactate dehydrogenase (LDH) were evaluated. PBMCs from prediabetic and diabetic patients exhibited increased sensitivity for T3 regarding elevated level of DNA damage, inhibition of catalase, and increase of TBARS and LDH. PBMCs from obese patients reacted in the same manner, except for DNA damage. The results of this study should contribute to a better understanding of the role of thyroid hormones in the progression of T2DM.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro",
volume = "23, 16",
pages = "9072",
doi = "10.3390/ijms23169072"
}

Đelić, N., Borozan, S., Dimitrijević-Srećković, V., Pajović, N., Mirilović, M., Stopper, H.,& Stanimirović, Z.. (2022). Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro. in International Journal of Molecular Sciences
MDPI., 23, 9072.
https://doi.org/10.3390/ijms23169072

Đelić N, Borozan S, Dimitrijević-Srećković V, Pajović N, Mirilović M, Stopper H, Stanimirović Z. Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro. in International Journal of Molecular Sciences. 2022;23:9072.
doi:10.3390/ijms23169072 .

Đelić, Ninoslav, Borozan, Sunčica, Dimitrijević-Srećković, Vesna, Pajović, Nevena, Mirilović, Milorad, Stopper, Helga, Stanimirović, Zoran, "Oxidative Stress and DNA Damage in Peripheral Blood Mononuclear Cells from Normal, Obese, Prediabetic and Diabetic Persons Exposed to Thyroid Hormone In Vitro" in International Journal of Molecular Sciences, 23 (2022):9072,
https://doi.org/10.3390/ijms23169072 . .

TY  - JOUR
AU  - Milić, Mirta
AU  - Ceppi, Marcello
AU  - Bruzzone, Marco
AU  - Azqueta, Amaya
AU  - Brunborg, Gunnar
AU  - Godschalk, Roger
AU  - Koppen, Gudrun
AU  - Langie, Sabine
AU  - Møller, Peter
AU  - Teixeira, João Paulo
AU  - Alija, Avdulla
AU  - Anderson, Diana
AU  - Andrade, Vanessa
AU  - Andreoli, Cristina
AU  - Asllani, Fisnik
AU  - Bangkoglu, Ezgi Eyluel
AU  - Barančoková, Magdalena
AU  - Basaran, Nursen
AU  - Boutet-Robinet, Elisa
AU  - Buschini, Annamaria
AU  - Cavallo, Delia
AU  - Costa Pereira, Cristiana
AU  - Costa, Carla
AU  - Costa, Solange
AU  - Da Silva, Juliana
AU  - Del Boˊ, Cristian
AU  - Dimitrijević Srećković, Vesna
AU  - Đelić, Ninoslav
AU  - Dobrzyńska, Malgorzata
AU  - Duračková, Zdenka
AU  - Dvořáková, Monika
AU  - Gajski, Goran
AU  - Galati, Serena
AU  - García Lima, Omar
AU  - Giovannelli, Lisa
AU  - Goroshinskaya, Irina A.
AU  - Grindel, Annemarie
AU  - Gutzkow, Kristine B.
AU  - Hernández, Alba
AU  - Hernández, Carlos
AU  - Holven, Kirsten B.
AU  - Ibero-Baraibar, Idoia
AU  - Ottestad, Inger
AU  - Kadioglu, Ela
AU  - Kažimirová, Alena
AU  - Kuznetsova, Elena
AU  - Ladeira, Carina
AU  - Laffon, Blanca
AU  - Lamonaca, Palma
AU  - Lebailly, Pierre
AU  - Louro, Henriqueta
AU  - Mandina Cardoso, Tania
AU  - Marcon, Francesca
AU  - Marcos, Ricard
AU  - Moretti, Massimo
AU  - Moretti, Silvia
AU  - Najafzadeh, Mojgan
AU  - Nemeth, Zsuzsanna
AU  - Neri, Monica
AU  - Novotna, Bozena
AU  - Orlow, Irene
AU  - Paduchova, Zuzana
AU  - Pastor, Susana
AU  - Perdry, Hervé
AU  - Spremo-Potparević, Biljana
AU  - Ramadhani, Dwi
AU  - Riso, Patrizia
AU  - Rohr, Paula
AU  - Rojas, Emilio
AU  - Rossner, Pavel
AU  - Safar, Anna
AU  - Sardas, Semra
AU  - Silva, Maria João
AU  - Sirota, Nikolay
AU  - Smolkova, Bozena
AU  - Staruchova, Marta
AU  - Stetina, Rudolf
AU  - Stopper, Helga
AU  - Surikova, Ekaterina I.
AU  - Ulven, Stine M.
AU  - Ursini, Cinzia Lucia
AU  - Valdiglesias, Vanessa
AU  - Valverde, Mahara
AU  - Vodicka, Pavel
AU  - Volkovova, Katarina
AU  - Wagner, Karl-Heinz
AU  - Živković, Lada
AU  - Dušinská, Maria
AU  - Collins, Andrew R.
AU  - Bonassi, Stefano
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2020
AB  - The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.
PB  - Elsevier
T2  - Mutation Research/Reviews in Mutation Research
T1  - The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
VL  - 787
SP  - 108371
DO  - 10.1016/j.mrrev.2021.108371
ER  - 

@article{
author = "Milić, Mirta and Ceppi, Marcello and Bruzzone, Marco and Azqueta, Amaya and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine and Møller, Peter and Teixeira, João Paulo and Alija, Avdulla and Anderson, Diana and Andrade, Vanessa and Andreoli, Cristina and Asllani, Fisnik and Bangkoglu, Ezgi Eyluel and Barančoková, Magdalena and Basaran, Nursen and Boutet-Robinet, Elisa and Buschini, Annamaria and Cavallo, Delia and Costa Pereira, Cristiana and Costa, Carla and Costa, Solange and Da Silva, Juliana and Del Boˊ, Cristian and Dimitrijević Srećković, Vesna and Đelić, Ninoslav and Dobrzyńska, Malgorzata and Duračková, Zdenka and Dvořáková, Monika and Gajski, Goran and Galati, Serena and García Lima, Omar and Giovannelli, Lisa and Goroshinskaya, Irina A. and Grindel, Annemarie and Gutzkow, Kristine B. and Hernández, Alba and Hernández, Carlos and Holven, Kirsten B. and Ibero-Baraibar, Idoia and Ottestad, Inger and Kadioglu, Ela and Kažimirová, Alena and Kuznetsova, Elena and Ladeira, Carina and Laffon, Blanca and Lamonaca, Palma and Lebailly, Pierre and Louro, Henriqueta and Mandina Cardoso, Tania and Marcon, Francesca and Marcos, Ricard and Moretti, Massimo and Moretti, Silvia and Najafzadeh, Mojgan and Nemeth, Zsuzsanna and Neri, Monica and Novotna, Bozena and Orlow, Irene and Paduchova, Zuzana and Pastor, Susana and Perdry, Hervé and Spremo-Potparević, Biljana and Ramadhani, Dwi and Riso, Patrizia and Rohr, Paula and Rojas, Emilio and Rossner, Pavel and Safar, Anna and Sardas, Semra and Silva, Maria João and Sirota, Nikolay and Smolkova, Bozena and Staruchova, Marta and Stetina, Rudolf and Stopper, Helga and Surikova, Ekaterina I. and Ulven, Stine M. and Ursini, Cinzia Lucia and Valdiglesias, Vanessa and Valverde, Mahara and Vodicka, Pavel and Volkovova, Katarina and Wagner, Karl-Heinz and Živković, Lada and Dušinská, Maria and Collins, Andrew R. and Bonassi, Stefano",
year = "2021",
abstract = "The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.",
publisher = "Elsevier",
journal = "Mutation Research/Reviews in Mutation Research",
title = "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders",
volume = "787",
pages = "108371",
doi = "10.1016/j.mrrev.2021.108371"
}

Milić, M., Ceppi, M., Bruzzone, M., Azqueta, A., Brunborg, G., Godschalk, R., Koppen, G., Langie, S., Møller, P., Teixeira, J. P., Alija, A., Anderson, D., Andrade, V., Andreoli, C., Asllani, F., Bangkoglu, E. E., Barančoková, M., Basaran, N., Boutet-Robinet, E., Buschini, A., Cavallo, D., Costa Pereira, C., Costa, C., Costa, S., Da Silva, J., Del Boˊ, C., Dimitrijević Srećković, V., Đelić, N., Dobrzyńska, M., Duračková, Z., Dvořáková, M., Gajski, G., Galati, S., García Lima, O., Giovannelli, L., Goroshinskaya, I. A., Grindel, A., Gutzkow, K. B., Hernández, A., Hernández, C., Holven, K. B., Ibero-Baraibar, I., Ottestad, I., Kadioglu, E., Kažimirová, A., Kuznetsova, E., Ladeira, C., Laffon, B., Lamonaca, P., Lebailly, P., Louro, H., Mandina Cardoso, T., Marcon, F., Marcos, R., Moretti, M., Moretti, S., Najafzadeh, M., Nemeth, Z., Neri, M., Novotna, B., Orlow, I., Paduchova, Z., Pastor, S., Perdry, H., Spremo-Potparević, B., Ramadhani, D., Riso, P., Rohr, P., Rojas, E., Rossner, P., Safar, A., Sardas, S., Silva, M. J., Sirota, N., Smolkova, B., Staruchova, M., Stetina, R., Stopper, H., Surikova, E. I., Ulven, S. M., Ursini, C. L., Valdiglesias, V., Valverde, M., Vodicka, P., Volkovova, K., Wagner, K., Živković, L., Dušinská, M., Collins, A. R.,& Bonassi, S.. (2021). The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research/Reviews in Mutation Research
Elsevier., 787, 108371.
https://doi.org/10.1016/j.mrrev.2021.108371

Milić M, Ceppi M, Bruzzone M, Azqueta A, Brunborg G, Godschalk R, Koppen G, Langie S, Møller P, Teixeira JP, Alija A, Anderson D, Andrade V, Andreoli C, Asllani F, Bangkoglu EE, Barančoková M, Basaran N, Boutet-Robinet E, Buschini A, Cavallo D, Costa Pereira C, Costa C, Costa S, Da Silva J, Del Boˊ C, Dimitrijević Srećković V, Đelić N, Dobrzyńska M, Duračková Z, Dvořáková M, Gajski G, Galati S, García Lima O, Giovannelli L, Goroshinskaya IA, Grindel A, Gutzkow KB, Hernández A, Hernández C, Holven KB, Ibero-Baraibar I, Ottestad I, Kadioglu E, Kažimirová A, Kuznetsova E, Ladeira C, Laffon B, Lamonaca P, Lebailly P, Louro H, Mandina Cardoso T, Marcon F, Marcos R, Moretti M, Moretti S, Najafzadeh M, Nemeth Z, Neri M, Novotna B, Orlow I, Paduchova Z, Pastor S, Perdry H, Spremo-Potparević B, Ramadhani D, Riso P, Rohr P, Rojas E, Rossner P, Safar A, Sardas S, Silva MJ, Sirota N, Smolkova B, Staruchova M, Stetina R, Stopper H, Surikova EI, Ulven SM, Ursini CL, Valdiglesias V, Valverde M, Vodicka P, Volkovova K, Wagner K, Živković L, Dušinská M, Collins AR, Bonassi S. The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research/Reviews in Mutation Research. 2021;787:108371.
doi:10.1016/j.mrrev.2021.108371 .

Milić, Mirta, Ceppi, Marcello, Bruzzone, Marco, Azqueta, Amaya, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine, Møller, Peter, Teixeira, João Paulo, Alija, Avdulla, Anderson, Diana, Andrade, Vanessa, Andreoli, Cristina, Asllani, Fisnik, Bangkoglu, Ezgi Eyluel, Barančoková, Magdalena, Basaran, Nursen, Boutet-Robinet, Elisa, Buschini, Annamaria, Cavallo, Delia, Costa Pereira, Cristiana, Costa, Carla, Costa, Solange, Da Silva, Juliana, Del Boˊ, Cristian, Dimitrijević Srećković, Vesna, Đelić, Ninoslav, Dobrzyńska, Malgorzata, Duračková, Zdenka, Dvořáková, Monika, Gajski, Goran, Galati, Serena, García Lima, Omar, Giovannelli, Lisa, Goroshinskaya, Irina A., Grindel, Annemarie, Gutzkow, Kristine B., Hernández, Alba, Hernández, Carlos, Holven, Kirsten B., Ibero-Baraibar, Idoia, Ottestad, Inger, Kadioglu, Ela, Kažimirová, Alena, Kuznetsova, Elena, Ladeira, Carina, Laffon, Blanca, Lamonaca, Palma, Lebailly, Pierre, Louro, Henriqueta, Mandina Cardoso, Tania, Marcon, Francesca, Marcos, Ricard, Moretti, Massimo, Moretti, Silvia, Najafzadeh, Mojgan, Nemeth, Zsuzsanna, Neri, Monica, Novotna, Bozena, Orlow, Irene, Paduchova, Zuzana, Pastor, Susana, Perdry, Hervé, Spremo-Potparević, Biljana, Ramadhani, Dwi, Riso, Patrizia, Rohr, Paula, Rojas, Emilio, Rossner, Pavel, Safar, Anna, Sardas, Semra, Silva, Maria João, Sirota, Nikolay, Smolkova, Bozena, Staruchova, Marta, Stetina, Rudolf, Stopper, Helga, Surikova, Ekaterina I., Ulven, Stine M., Ursini, Cinzia Lucia, Valdiglesias, Vanessa, Valverde, Mahara, Vodicka, Pavel, Volkovova, Katarina, Wagner, Karl-Heinz, Živković, Lada, Dušinská, Maria, Collins, Andrew R., Bonassi, Stefano, "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders" in Mutation Research/Reviews in Mutation Research, 787 (2021):108371,
https://doi.org/10.1016/j.mrrev.2021.108371 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Borozan, Sunčica
AU  - Dimirijević-Srećković, Vesna
AU  - Pajović, Nevena
AU  - Vejnović, Branislav
AU  - Borozan, Nevena
AU  - Bankoglu, Ezgi Eylül
AU  - Stopper, Helga
AU  - Stanimirović, Zoran
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2546
AB  - Diabetes represents one of the major health concerns, especially in developed countries. Some hormones such as
the stress hormone adrenaline can induce reactive oxygen species (ROS) and may worsen the diabetes.
Therefore, the main aim of the investigation was to find out whether peripheral blood mononuclear cells
(PBMCs) from normal persons have less DNA damage induced by adrenaline (0.1, 1 and 10 μM) in comparison to
PBMCs from obese, prediabetic and diabetic patients. Also, the biochemical parameters of oxidative stress
(TBARS, catalase) and lactate dehydrogenase were monitored. It was observed that higher concentrations of
adrenaline (1 and 10 μM) induced DNA damage in the obese, prediabetic and diabetic groups. In healthy individuals only the highest concentration of adrenaline caused significant increase in the DNA damage. In
summary, total comet score (TCS) comparison has shown significant differences between groups, and DNA
damaging effects of adrenaline were most evident in diabetic patients. The results of the biochemical analysis
also demonstrate that adrenaline exerts most obvious effects in diabetic individuals which is manifested as
significant change of parameters of oxidative stress. In summary, the obtained results demonstrated that diabetics are more sensitive to genotoxic effects of adrenaline and this effect probably resulted from decreased
antioxidative defence mechanisms in various stages of progression through diabetes. Therefore, these results
could contribute to a better understanding of a role of endocrine factors to damage of cellular biomolecules
which could be useful in finding novel therapeutic approaches and lifestyle changes with an aim to lower the
possibility of diabetes complications.
PB  - Elsevier
T2  - Mutation Research: Genetic Toxicology and Environmental Mutagenesis
T1  - Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro
VL  - 843
SP  - 81
EP  - 89
DO  - 10.1016/j.mrgentox.2019.01.013
ER  - 

@article{
author = "Đelić, Ninoslav and Radaković, Milena and Borozan, Sunčica and Dimirijević-Srećković, Vesna and Pajović, Nevena and Vejnović, Branislav and Borozan, Nevena and Bankoglu, Ezgi Eylül and Stopper, Helga and Stanimirović, Zoran",
year = "2019",
abstract = "Diabetes represents one of the major health concerns, especially in developed countries. Some hormones such as
the stress hormone adrenaline can induce reactive oxygen species (ROS) and may worsen the diabetes.
Therefore, the main aim of the investigation was to find out whether peripheral blood mononuclear cells
(PBMCs) from normal persons have less DNA damage induced by adrenaline (0.1, 1 and 10 μM) in comparison to
PBMCs from obese, prediabetic and diabetic patients. Also, the biochemical parameters of oxidative stress
(TBARS, catalase) and lactate dehydrogenase were monitored. It was observed that higher concentrations of
adrenaline (1 and 10 μM) induced DNA damage in the obese, prediabetic and diabetic groups. In healthy individuals only the highest concentration of adrenaline caused significant increase in the DNA damage. In
summary, total comet score (TCS) comparison has shown significant differences between groups, and DNA
damaging effects of adrenaline were most evident in diabetic patients. The results of the biochemical analysis
also demonstrate that adrenaline exerts most obvious effects in diabetic individuals which is manifested as
significant change of parameters of oxidative stress. In summary, the obtained results demonstrated that diabetics are more sensitive to genotoxic effects of adrenaline and this effect probably resulted from decreased
antioxidative defence mechanisms in various stages of progression through diabetes. Therefore, these results
could contribute to a better understanding of a role of endocrine factors to damage of cellular biomolecules
which could be useful in finding novel therapeutic approaches and lifestyle changes with an aim to lower the
possibility of diabetes complications.",
publisher = "Elsevier",
journal = "Mutation Research: Genetic Toxicology and Environmental Mutagenesis",
title = "Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro",
volume = "843",
pages = "81-89",
doi = "10.1016/j.mrgentox.2019.01.013"
}

Đelić, N., Radaković, M., Borozan, S., Dimirijević-Srećković, V., Pajović, N., Vejnović, B., Borozan, N., Bankoglu, E. E., Stopper, H.,& Stanimirović, Z.. (2019). Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro. in Mutation Research: Genetic Toxicology and Environmental Mutagenesis
Elsevier., 843, 81-89.
https://doi.org/10.1016/j.mrgentox.2019.01.013

Đelić N, Radaković M, Borozan S, Dimirijević-Srećković V, Pajović N, Vejnović B, Borozan N, Bankoglu EE, Stopper H, Stanimirović Z. Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro. in Mutation Research: Genetic Toxicology and Environmental Mutagenesis. 2019;843:81-89.
doi:10.1016/j.mrgentox.2019.01.013 .

Đelić, Ninoslav, Radaković, Milena, Borozan, Sunčica, Dimirijević-Srećković, Vesna, Pajović, Nevena, Vejnović, Branislav, Borozan, Nevena, Bankoglu, Ezgi Eylül, Stopper, Helga, Stanimirović, Zoran, "Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to thyroid hormone in vitro" in Mutation Research: Genetic Toxicology and Environmental Mutagenesis, 843 (2019):81-89,
https://doi.org/10.1016/j.mrgentox.2019.01.013 . .

TY  - JOUR
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Borozan, Sunčica
AU  - Dimirijević-Srecković, Vesna
AU  - Pajović, Nevena
AU  - Vejnović, Branislav
AU  - Borozan, Nevena
AU  - Bankoglu, Ezgi Eylul
AU  - Stopper, Helga
AU  - Stanimirović, Zoran
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1780
AB  - Diabetes represents one of the major health concerns, especially In developed countries. Some hormones such as the stress hormone adrenaline can induce reactive oxygen species (ROS) and may worsen the diabetes. Therefore, the main aim of the investigation was to find out whether peripheral blood mononuclear cells (PBMCs) from normal persons have less DNA damage induced by adrenaline (0.1, 1 and 10 mu M) in comparison to PBMCs from obese, prediabetic and diabetic patients. Also, the biochemical parameters of oxidative stress (TBARS, catalase) and lactate dehydrogenase were monitored. It was observed that higher concentrations of adrenaline (1 and 10 mu M) induced DNA damage in the obese, prediabetic and diabetic groups. In healthy individuals only the highest concentration of adrenaline caused significant Increase in the DNA damage. In summary, total comet score (TCS) comparison has shown significant differences between groups, and DNA damaging effects of adrenaline were most evident in diabetic patients. The results of the biochemical analysis also demonstrate that adrenaline exerts most obvious effects in diabetic individuals which is manifested as significant change of parameters of oxidative stress. In summary, the obtained results demonstrated that diabetics are more sensitive to genotoxic effects of adrenaline and this effect probably resulted from decreased antioxidative defence mechanisms in various stages of progression through diabetes. Therefore, these results could contribute to a better understanding of a role of endocrine factors to damage of cellular biomolecules which could be useful in finding novel therapeutic approaches and lifestyle changes with an aim to lower the possibility of diabetes complications.
PB  - Elsevier, Amsterdam
T2  - Mutation Research-Genetic Toxicology and Environmental Mutagenesis
T1  - Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to adrenaline in vitro
VL  - 843
SP  - 81
EP  - 89
DO  - 10.1016/j.mrgentox.2019.01.013
ER  - 

@article{
author = "Đelić, Ninoslav and Radaković, Milena and Borozan, Sunčica and Dimirijević-Srecković, Vesna and Pajović, Nevena and Vejnović, Branislav and Borozan, Nevena and Bankoglu, Ezgi Eylul and Stopper, Helga and Stanimirović, Zoran",
year = "2019",
abstract = "Diabetes represents one of the major health concerns, especially In developed countries. Some hormones such as the stress hormone adrenaline can induce reactive oxygen species (ROS) and may worsen the diabetes. Therefore, the main aim of the investigation was to find out whether peripheral blood mononuclear cells (PBMCs) from normal persons have less DNA damage induced by adrenaline (0.1, 1 and 10 mu M) in comparison to PBMCs from obese, prediabetic and diabetic patients. Also, the biochemical parameters of oxidative stress (TBARS, catalase) and lactate dehydrogenase were monitored. It was observed that higher concentrations of adrenaline (1 and 10 mu M) induced DNA damage in the obese, prediabetic and diabetic groups. In healthy individuals only the highest concentration of adrenaline caused significant Increase in the DNA damage. In summary, total comet score (TCS) comparison has shown significant differences between groups, and DNA damaging effects of adrenaline were most evident in diabetic patients. The results of the biochemical analysis also demonstrate that adrenaline exerts most obvious effects in diabetic individuals which is manifested as significant change of parameters of oxidative stress. In summary, the obtained results demonstrated that diabetics are more sensitive to genotoxic effects of adrenaline and this effect probably resulted from decreased antioxidative defence mechanisms in various stages of progression through diabetes. Therefore, these results could contribute to a better understanding of a role of endocrine factors to damage of cellular biomolecules which could be useful in finding novel therapeutic approaches and lifestyle changes with an aim to lower the possibility of diabetes complications.",
publisher = "Elsevier, Amsterdam",
journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis",
title = "Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to adrenaline in vitro",
volume = "843",
pages = "81-89",
doi = "10.1016/j.mrgentox.2019.01.013"
}

Đelić, N., Radaković, M., Borozan, S., Dimirijević-Srecković, V., Pajović, N., Vejnović, B., Borozan, N., Bankoglu, E. E., Stopper, H.,& Stanimirović, Z.. (2019). Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to adrenaline in vitro. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis
Elsevier, Amsterdam., 843, 81-89.
https://doi.org/10.1016/j.mrgentox.2019.01.013

Đelić N, Radaković M, Borozan S, Dimirijević-Srecković V, Pajović N, Vejnović B, Borozan N, Bankoglu EE, Stopper H, Stanimirović Z. Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to adrenaline in vitro. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2019;843:81-89.
doi:10.1016/j.mrgentox.2019.01.013 .

Đelić, Ninoslav, Radaković, Milena, Borozan, Sunčica, Dimirijević-Srecković, Vesna, Pajović, Nevena, Vejnović, Branislav, Borozan, Nevena, Bankoglu, Ezgi Eylul, Stopper, Helga, Stanimirović, Zoran, "Oxidative stress and DNA damage in peripheral blood mononuclear cells from normal, obese, prediabetic and diabetic persons exposed to adrenaline in vitro" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 843 (2019):81-89,
https://doi.org/10.1016/j.mrgentox.2019.01.013 . .

TY  - JOUR
AU  - Montag, Gracia
AU  - Bankoglu, Ezgi Eylul
AU  - Bolte, Annika
AU  - Hintzsche, Henning
AU  - Đelić, Ninoslav
AU  - Stopper, Helga
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1789
AB  - The aim of this study was to investigate the effect of the cell differentiation status on the sensitivity to genotoxic insults. For this, we utilized the comet assay to test the DNA damage after treatment with 5 different substances with different mechanism of action in human promyelocytic HL60 cells with or without cell differentiation. A 4-hour MMS treatment induced a significant and concentration-dependent increase in DNA damage for both differentiated and undifferentiated cells, but the difference in sensitivity was only significant at the highest concentration. A 4-hour doxorubicin treatment did not induce DNA damage in differentiated HL60 cells, while it did in undifferentiated cells with its highest tested concentration. A one-hour etoposide treatment caused significant increase in DNA damage concentration dependently in both cell variants. This DNA damage was significantly higher in undifferentiated HL60 cells with several tested concentrations of etoposide. The treatment with the oxidizing substances hydrogen peroxide and potassium bromate yielded significant DNA damage induction in both undifferentiated and differentiated cells with no difference according to the differentiation status. Doxorubicin and etoposide are known to inhibit topoisomerase II. The activity of this enzyme has been shown to be higher in undifferentiated actively proliferating cells than in differentiated cells. This may be of relevance when exposures to topoisomerase-inhibiting compounds or the genotoxicity of compounds with unknown mechanism of action are assessed in routine testing.
PB  - Elsevier, Amsterdam
T2  - Mutation Research-Genetic Toxicology and Environmental Mutagenesis
T1  - Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay
VL  - 845
SP  - UNSP 402972
DO  - 10.1016/j.mrgentox.2018.10.004
ER  - 

@article{
author = "Montag, Gracia and Bankoglu, Ezgi Eylul and Bolte, Annika and Hintzsche, Henning and Đelić, Ninoslav and Stopper, Helga",
year = "2019",
abstract = "The aim of this study was to investigate the effect of the cell differentiation status on the sensitivity to genotoxic insults. For this, we utilized the comet assay to test the DNA damage after treatment with 5 different substances with different mechanism of action in human promyelocytic HL60 cells with or without cell differentiation. A 4-hour MMS treatment induced a significant and concentration-dependent increase in DNA damage for both differentiated and undifferentiated cells, but the difference in sensitivity was only significant at the highest concentration. A 4-hour doxorubicin treatment did not induce DNA damage in differentiated HL60 cells, while it did in undifferentiated cells with its highest tested concentration. A one-hour etoposide treatment caused significant increase in DNA damage concentration dependently in both cell variants. This DNA damage was significantly higher in undifferentiated HL60 cells with several tested concentrations of etoposide. The treatment with the oxidizing substances hydrogen peroxide and potassium bromate yielded significant DNA damage induction in both undifferentiated and differentiated cells with no difference according to the differentiation status. Doxorubicin and etoposide are known to inhibit topoisomerase II. The activity of this enzyme has been shown to be higher in undifferentiated actively proliferating cells than in differentiated cells. This may be of relevance when exposures to topoisomerase-inhibiting compounds or the genotoxicity of compounds with unknown mechanism of action are assessed in routine testing.",
publisher = "Elsevier, Amsterdam",
journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis",
title = "Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay",
volume = "845",
pages = "UNSP 402972",
doi = "10.1016/j.mrgentox.2018.10.004"
}

Montag, G., Bankoglu, E. E., Bolte, A., Hintzsche, H., Đelić, N.,& Stopper, H.. (2019). Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis
Elsevier, Amsterdam., 845, UNSP 402972.
https://doi.org/10.1016/j.mrgentox.2018.10.004

Montag G, Bankoglu EE, Bolte A, Hintzsche H, Đelić N, Stopper H. Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2019;845:UNSP 402972.
doi:10.1016/j.mrgentox.2018.10.004 .

Montag, Gracia, Bankoglu, Ezgi Eylul, Bolte, Annika, Hintzsche, Henning, Đelić, Ninoslav, Stopper, Helga, "Differentiated and exponentially growing HL60 cells exhibit different sensitivity to some genotoxic agents in the comet assay" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 845 (2019):UNSP 402972,
https://doi.org/10.1016/j.mrgentox.2018.10.004 . .