TY  - JOUR
AU  - Đorđević, Jasna
AU  - Ledina, Tijana
AU  - Golob, Majda
AU  - Mohar Lorbeg, Petra
AU  - Čanžek Majhenič, Andreja
AU  - Bogovič Matijašić, Bojana
AU  - Bulajić, Snežana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2713
AB  - Enterococci represent a significant part of the non-starter LAB microbiota of artisanal cheeses produced mainly from raw milk. Common approaches to safety evaluation of enterococci isolates include assessment of antimicrobial resistance and virulence potential. Hence, a collection of 47 (n = 22, Serbia; n = 25, Slovenia) dairy enterococcal isolates, of which E. faecalis (n = 28), E. faecium (n = 11), E. durans (n = 5), E. casseliflavus (n = 2), and E. gallinarum (n = 1), was analyzed. The susceptibility to 12 antimicrobials was tested using a broth microdilution method, and the presence of the selected antimicrobial resistance and virulence genes was investigated using PCR. Isolates were resistant to tetracycline (TET) (25.5%), erythromycin (ERY) (17.0%), gentamycin and chloramphenicol (CHL) (∼6%). No resistance to ampicillin (AMP), ciprofloxacin (CIP), daptomycin (DAP), linezolid (LZD), teicoplanin (TEI), tigecycline (TGC) and vancomycin (VAN) was detected. Among all the resistance determinants analyzed, ermB gene was detected most frequently. All 10 virulence genes analyzed were detected with a distribution of cpd (72.3%), cob and ccf (70.2%), gelE (68.1%), hyl (59.6%), agg (53.2%) and esp (46.8%). The genes encoding cytolysin (cylA, cylM and cylB) were amplified to a lesser extent (21.3%, 21.3% and 12.8%, respectively). However, due to the limited number of enterococci isolates analyzed in the present study, further studies are still required in order to better document the safety status of dairy enterococci.
PB  - SAGE Publications
T2  - Food Science and Technology International
T1  - Safety evaluation of enterococci isolated from raw milk and artisanal cheeses made in Slovenia and Serbia
SP  - 1
EP  - 11
DO  - 10.1177/10820132221117870
ER  - 

@article{
author = "Đorđević, Jasna and Ledina, Tijana and Golob, Majda and Mohar Lorbeg, Petra and Čanžek Majhenič, Andreja and Bogovič Matijašić, Bojana and Bulajić, Snežana",
year = "2022",
abstract = "Enterococci represent a significant part of the non-starter LAB microbiota of artisanal cheeses produced mainly from raw milk. Common approaches to safety evaluation of enterococci isolates include assessment of antimicrobial resistance and virulence potential. Hence, a collection of 47 (n = 22, Serbia; n = 25, Slovenia) dairy enterococcal isolates, of which E. faecalis (n = 28), E. faecium (n = 11), E. durans (n = 5), E. casseliflavus (n = 2), and E. gallinarum (n = 1), was analyzed. The susceptibility to 12 antimicrobials was tested using a broth microdilution method, and the presence of the selected antimicrobial resistance and virulence genes was investigated using PCR. Isolates were resistant to tetracycline (TET) (25.5%), erythromycin (ERY) (17.0%), gentamycin and chloramphenicol (CHL) (∼6%). No resistance to ampicillin (AMP), ciprofloxacin (CIP), daptomycin (DAP), linezolid (LZD), teicoplanin (TEI), tigecycline (TGC) and vancomycin (VAN) was detected. Among all the resistance determinants analyzed, ermB gene was detected most frequently. All 10 virulence genes analyzed were detected with a distribution of cpd (72.3%), cob and ccf (70.2%), gelE (68.1%), hyl (59.6%), agg (53.2%) and esp (46.8%). The genes encoding cytolysin (cylA, cylM and cylB) were amplified to a lesser extent (21.3%, 21.3% and 12.8%, respectively). However, due to the limited number of enterococci isolates analyzed in the present study, further studies are still required in order to better document the safety status of dairy enterococci.",
publisher = "SAGE Publications",
journal = "Food Science and Technology International",
title = "Safety evaluation of enterococci isolated from raw milk and artisanal cheeses made in Slovenia and Serbia",
pages = "1-11",
doi = "10.1177/10820132221117870"
}

Đorđević, J., Ledina, T., Golob, M., Mohar Lorbeg, P., Čanžek Majhenič, A., Bogovič Matijašić, B.,& Bulajić, S.. (2022). Safety evaluation of enterococci isolated from raw milk and artisanal cheeses made in Slovenia and Serbia. in Food Science and Technology International
SAGE Publications., 1-11.
https://doi.org/10.1177/10820132221117870

Đorđević J, Ledina T, Golob M, Mohar Lorbeg P, Čanžek Majhenič A, Bogovič Matijašić B, Bulajić S. Safety evaluation of enterococci isolated from raw milk and artisanal cheeses made in Slovenia and Serbia. in Food Science and Technology International. 2022;:1-11.
doi:10.1177/10820132221117870 .

Đorđević, Jasna, Ledina, Tijana, Golob, Majda, Mohar Lorbeg, Petra, Čanžek Majhenič, Andreja, Bogovič Matijašić, Bojana, Bulajić, Snežana, "Safety evaluation of enterococci isolated from raw milk and artisanal cheeses made in Slovenia and Serbia" in Food Science and Technology International (2022):1-11,
https://doi.org/10.1177/10820132221117870 . .