TY  - JOUR
AU  - Bošković Cabrol, Marija
AU  - Glišić, Milica
AU  - Baltić, Milan Ž.
AU  - Jovanović, Dragoljub
AU  - Silađi, Čaba
AU  - Simunović, Stefan
AU  - Tomašević, Igor
AU  - Raymundo, Anabela
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2543
AB  - This study aimed to evaluate the effect of the chlorophyll-deficient microalgae mutants, honey (yellow) and white Chlorella vulgaris, (3%) on the nutritional, physicochemical, microbiological, and sensory characteristics of frankfurters. The presence of microalgae resulted in increased PUFA content and higher PUFA/SFA ratio, but lower n-6/n-3 ratio and lipid indices (P < 0.05). C. vulgaris inclusion in frankfurters increased (P < 0.05) Na, K, Ca, P, and Zn and improved the Na/K ratio, but lowered Mn, and in the case of white C. vulgaris, Cu content, compared to the control. The higher protein content decreased water release from emulsions elaborated with microalgae. White C. vulgaris inclusion decreased cohesiveness and springiness of the frankfurters. Due to the presence of pigment, microalgae inclusion led to a decrease in redness and an increase in yellowness of frankfurters. The presence of microalgae resulted in lower (P < 0.05) bacterial counts and did not affect TBARs during storage. The addition of microalgae in frankfurters produced acceptable sensory characteristics but resulted in lower scores compared to reference products.
PB  - Elsevier
T2  - Meat Science
T1  - White and honey Chlorella vulgaris: Sustainable ingredients with the potential to improve nutritional value of pork frankfurters without compromising quality
VL  - 198
SP  - 109123
DO  - 10.1016/j.meatsci.2023.109123
ER  - 

@article{
author = "Bošković Cabrol, Marija and Glišić, Milica and Baltić, Milan Ž. and Jovanović, Dragoljub and Silađi, Čaba and Simunović, Stefan and Tomašević, Igor and Raymundo, Anabela",
year = "2023",
abstract = "This study aimed to evaluate the effect of the chlorophyll-deficient microalgae mutants, honey (yellow) and white Chlorella vulgaris, (3%) on the nutritional, physicochemical, microbiological, and sensory characteristics of frankfurters. The presence of microalgae resulted in increased PUFA content and higher PUFA/SFA ratio, but lower n-6/n-3 ratio and lipid indices (P < 0.05). C. vulgaris inclusion in frankfurters increased (P < 0.05) Na, K, Ca, P, and Zn and improved the Na/K ratio, but lowered Mn, and in the case of white C. vulgaris, Cu content, compared to the control. The higher protein content decreased water release from emulsions elaborated with microalgae. White C. vulgaris inclusion decreased cohesiveness and springiness of the frankfurters. Due to the presence of pigment, microalgae inclusion led to a decrease in redness and an increase in yellowness of frankfurters. The presence of microalgae resulted in lower (P < 0.05) bacterial counts and did not affect TBARs during storage. The addition of microalgae in frankfurters produced acceptable sensory characteristics but resulted in lower scores compared to reference products.",
publisher = "Elsevier",
journal = "Meat Science",
title = "White and honey Chlorella vulgaris: Sustainable ingredients with the potential to improve nutritional value of pork frankfurters without compromising quality",
volume = "198",
pages = "109123",
doi = "10.1016/j.meatsci.2023.109123"
}

Bošković Cabrol, M., Glišić, M., Baltić, M. Ž., Jovanović, D., Silađi, Č., Simunović, S., Tomašević, I.,& Raymundo, A.. (2023). White and honey Chlorella vulgaris: Sustainable ingredients with the potential to improve nutritional value of pork frankfurters without compromising quality. in Meat Science
Elsevier., 198, 109123.
https://doi.org/10.1016/j.meatsci.2023.109123

Bošković Cabrol M, Glišić M, Baltić MŽ, Jovanović D, Silađi Č, Simunović S, Tomašević I, Raymundo A. White and honey Chlorella vulgaris: Sustainable ingredients with the potential to improve nutritional value of pork frankfurters without compromising quality. in Meat Science. 2023;198:109123.
doi:10.1016/j.meatsci.2023.109123 .

Bošković Cabrol, Marija, Glišić, Milica, Baltić, Milan Ž., Jovanović, Dragoljub, Silađi, Čaba, Simunović, Stefan, Tomašević, Igor, Raymundo, Anabela, "White and honey Chlorella vulgaris: Sustainable ingredients with the potential to improve nutritional value of pork frankfurters without compromising quality" in Meat Science, 198 (2023):109123,
https://doi.org/10.1016/j.meatsci.2023.109123 . .

TY  - JOUR
AU  - Bošković Cabrol, Marija
AU  - Martins, Joana C.
AU  - Malhão, Leonardo P.
AU  - Alfaia, Cristina M.
AU  - Prates, José A. M.
AU  - Almeida, André M.
AU  - Lordelo, Madalena
AU  - Raymundo, Anabela
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2454
AB  - The incorporation of sustainable protein sources in animal feeding is a growing trend. So far, no study has investigated in vitro digestion of meat, from broilers fed microalgae, in a human model. This research aimed to evaluate the effect of incorporating Chlorella vulgaris in the broilers diet on human protein digestibility, and mineral bioaccessibility. The study used 240 male Ross 308 broilers randomly allocated to groups fed a control diet or a diet where soybean meal was replaced with 10% (CV10%), 15% (CV15%), or 20% (CV15%) of C. vulgaris for 40 days. The microalga supplementation increased the protein and lowered the fat content in the muscle. Results on the percentages of amino acids highlighted that arginine and threonine proportions increased and lysine and cysteine proportions decreased with microalga inclusion. CV15% and CV20% meat had higher amount of K, Ca, Mg, P, and Fe in raw breasts, improving the nutrient composition of the meat. Cooking caused a decrease in Na and K and an increase in other minerals. CV20% had higher bioaccessibility of K, Ca, Mg, P, and Mg, compared to the control. Replacing soybean meal in broiler feed with higher concentrations of C. vugaris could improve the digestibility of meat protein and minerals.
PB  - MDPI
T2  - Foods
T1  - Digestibility of Meat Mineral and Proteins from Broilers Fed with Graded Levels of Chlorella vulgaris
VL  - 11
IS  - 9
SP  - 1345
DO  - 10.3390/foods11091345
ER  - 

@article{
author = "Bošković Cabrol, Marija and Martins, Joana C. and Malhão, Leonardo P. and Alfaia, Cristina M. and Prates, José A. M. and Almeida, André M. and Lordelo, Madalena and Raymundo, Anabela",
year = "2022",
abstract = "The incorporation of sustainable protein sources in animal feeding is a growing trend. So far, no study has investigated in vitro digestion of meat, from broilers fed microalgae, in a human model. This research aimed to evaluate the effect of incorporating Chlorella vulgaris in the broilers diet on human protein digestibility, and mineral bioaccessibility. The study used 240 male Ross 308 broilers randomly allocated to groups fed a control diet or a diet where soybean meal was replaced with 10% (CV10%), 15% (CV15%), or 20% (CV15%) of C. vulgaris for 40 days. The microalga supplementation increased the protein and lowered the fat content in the muscle. Results on the percentages of amino acids highlighted that arginine and threonine proportions increased and lysine and cysteine proportions decreased with microalga inclusion. CV15% and CV20% meat had higher amount of K, Ca, Mg, P, and Fe in raw breasts, improving the nutrient composition of the meat. Cooking caused a decrease in Na and K and an increase in other minerals. CV20% had higher bioaccessibility of K, Ca, Mg, P, and Mg, compared to the control. Replacing soybean meal in broiler feed with higher concentrations of C. vugaris could improve the digestibility of meat protein and minerals.",
publisher = "MDPI",
journal = "Foods",
title = "Digestibility of Meat Mineral and Proteins from Broilers Fed with Graded Levels of Chlorella vulgaris",
volume = "11",
number = "9",
pages = "1345",
doi = "10.3390/foods11091345"
}

Bošković Cabrol, M., Martins, J. C., Malhão, L. P., Alfaia, C. M., Prates, J. A. M., Almeida, A. M., Lordelo, M.,& Raymundo, A.. (2022). Digestibility of Meat Mineral and Proteins from Broilers Fed with Graded Levels of Chlorella vulgaris. in Foods
MDPI., 11(9), 1345.
https://doi.org/10.3390/foods11091345

Bošković Cabrol M, Martins JC, Malhão LP, Alfaia CM, Prates JAM, Almeida AM, Lordelo M, Raymundo A. Digestibility of Meat Mineral and Proteins from Broilers Fed with Graded Levels of Chlorella vulgaris. in Foods. 2022;11(9):1345.
doi:10.3390/foods11091345 .

Bošković Cabrol, Marija, Martins, Joana C., Malhão, Leonardo P., Alfaia, Cristina M., Prates, José A. M., Almeida, André M., Lordelo, Madalena, Raymundo, Anabela, "Digestibility of Meat Mineral and Proteins from Broilers Fed with Graded Levels of Chlorella vulgaris" in Foods, 11, no. 9 (2022):1345,
https://doi.org/10.3390/foods11091345 . .

TY  - JOUR
AU  - Bošković Cabrol, Marija
AU  - Martins, Joana C.
AU  - Malhão, Leonardo P.
AU  - Alves, Susana P.
AU  - Bessa, Rui J. B.
AU  - Almeida, André M.
AU  - Raymundo, Anabela
AU  - Lordelo, Madalena
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2465
AB  - Alternative feed ingredients, such as microalgae, may be more sustainable in comparison to conventional feedstuffs that need large amounts of arable land and are often imported. This study evaluates the effects of Chlorella vulgaris various inclusion levels in the diet of broiler chickens on performance, carcass yield, organ measurements, breast meat quality, fatty acids profile, and antioxidant capacity. A total of two hundred forty 5 d old male Ross 308 broilers were randomly allotted to 4 groups (6 replicates of 10 birds each). Each group received either a control diet or a diet where soybean meal was replaced with 10% (CV10%), 15% (CV15%), or 20% C. vulgaris for 40 d. Performance parameters, carcass and meat traits were evaluated. Compared to the control group, birds supplemented with C. vulgaris (15% and 20%) had lower body weight, weight gain, and feed intake (P < 0.0001), whereas no differences were observed between the control and CV10% groups (P > 0.05). Feed conversion ratio did not differ between control and CV groups. Diets containing C. vulgaris significantly increased ileal digesta viscosity, weight and size of several gastrointestinal compartments, as well as breast muscle yield (P < 0.0001). Incorporation of C. vulgaris resulted in yellower breast muscle (P < 0.0001), with significantly increased chlorophyll a (P < 0.05), chlorophyll b, and total carotenoids contents (P < 0.0001). Inclusion of C. vulgaris decreased bacterial count in meat samples in comparison to controls (P < 0.0001). A 20% C. vulgaris inclusion resulted in higher water holding capacity (P < 0.05) and lower cooking loss (P < 0.05). As dietary C. vulgaris increased, concentrations of DHA + EPA (P < 0.05) and n-3 PUFA (P < 0.0001) increased in breast meat, while the n-6/n-3 PUFA ratio decreased (P < 0.0001). Sensory analysis showed that breast meat from the CV10% group had the highest acceptance score. Overall, dietary concentrations of C. vulgaris of up to 20% improve breast meat quality, whereas 10% of C. vulgaris inclusion is recommended.
PB  - Elsevier
T2  - Poultry Science
T1  - Partial replacement of soybean meal with Chlorella vulgaris in broiler diets influences performance and improves breast meat quality and fatty acid composition
VL  - 101
IS  - 8
SP  - 101955
DO  - 10.1016/j.psj.2022.101955
ER  - 

@article{
author = "Bošković Cabrol, Marija and Martins, Joana C. and Malhão, Leonardo P. and Alves, Susana P. and Bessa, Rui J. B. and Almeida, André M. and Raymundo, Anabela and Lordelo, Madalena",
year = "2022",
abstract = "Alternative feed ingredients, such as microalgae, may be more sustainable in comparison to conventional feedstuffs that need large amounts of arable land and are often imported. This study evaluates the effects of Chlorella vulgaris various inclusion levels in the diet of broiler chickens on performance, carcass yield, organ measurements, breast meat quality, fatty acids profile, and antioxidant capacity. A total of two hundred forty 5 d old male Ross 308 broilers were randomly allotted to 4 groups (6 replicates of 10 birds each). Each group received either a control diet or a diet where soybean meal was replaced with 10% (CV10%), 15% (CV15%), or 20% C. vulgaris for 40 d. Performance parameters, carcass and meat traits were evaluated. Compared to the control group, birds supplemented with C. vulgaris (15% and 20%) had lower body weight, weight gain, and feed intake (P < 0.0001), whereas no differences were observed between the control and CV10% groups (P > 0.05). Feed conversion ratio did not differ between control and CV groups. Diets containing C. vulgaris significantly increased ileal digesta viscosity, weight and size of several gastrointestinal compartments, as well as breast muscle yield (P < 0.0001). Incorporation of C. vulgaris resulted in yellower breast muscle (P < 0.0001), with significantly increased chlorophyll a (P < 0.05), chlorophyll b, and total carotenoids contents (P < 0.0001). Inclusion of C. vulgaris decreased bacterial count in meat samples in comparison to controls (P < 0.0001). A 20% C. vulgaris inclusion resulted in higher water holding capacity (P < 0.05) and lower cooking loss (P < 0.05). As dietary C. vulgaris increased, concentrations of DHA + EPA (P < 0.05) and n-3 PUFA (P < 0.0001) increased in breast meat, while the n-6/n-3 PUFA ratio decreased (P < 0.0001). Sensory analysis showed that breast meat from the CV10% group had the highest acceptance score. Overall, dietary concentrations of C. vulgaris of up to 20% improve breast meat quality, whereas 10% of C. vulgaris inclusion is recommended.",
publisher = "Elsevier",
journal = "Poultry Science",
title = "Partial replacement of soybean meal with Chlorella vulgaris in broiler diets influences performance and improves breast meat quality and fatty acid composition",
volume = "101",
number = "8",
pages = "101955",
doi = "10.1016/j.psj.2022.101955"
}

Bošković Cabrol, M., Martins, J. C., Malhão, L. P., Alves, S. P., Bessa, R. J. B., Almeida, A. M., Raymundo, A.,& Lordelo, M.. (2022). Partial replacement of soybean meal with Chlorella vulgaris in broiler diets influences performance and improves breast meat quality and fatty acid composition. in Poultry Science
Elsevier., 101(8), 101955.
https://doi.org/10.1016/j.psj.2022.101955

Bošković Cabrol M, Martins JC, Malhão LP, Alves SP, Bessa RJB, Almeida AM, Raymundo A, Lordelo M. Partial replacement of soybean meal with Chlorella vulgaris in broiler diets influences performance and improves breast meat quality and fatty acid composition. in Poultry Science. 2022;101(8):101955.
doi:10.1016/j.psj.2022.101955 .

Bošković Cabrol, Marija, Martins, Joana C., Malhão, Leonardo P., Alves, Susana P., Bessa, Rui J. B., Almeida, André M., Raymundo, Anabela, Lordelo, Madalena, "Partial replacement of soybean meal with Chlorella vulgaris in broiler diets influences performance and improves breast meat quality and fatty acid composition" in Poultry Science, 101, no. 8 (2022):101955,
https://doi.org/10.1016/j.psj.2022.101955 . .

TY  - CONF
AU  - Bošković Cabrol, Marija
AU  - Glišić, Milica
AU  - Baltić, Milan Ž.
AU  - Jovanović, Dragoljub
AU  - Silađi, Čaba
AU  - Simunović, Stefan
AU  - Tomašević, Igor
AU  - Raymundo, Anabela
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2569
AB  - Microalgae have been proven to be a promising functional and sustainable ingredient in different food matrices, including some meat products [1,2,3,4,5,6]. However, the coloring of meat products containing microalgae Spirulina maxima and Chlorella vulgaris in a dark green could lead to consumers` rejection of these products. To overcome the color barrier, we used white and yellow chlorophyll-deficient C. vulgaris mutants, commercially available (Honey Chlorella), in designing acceptable products. The study aimed to analyze the nutritional composition (chemical, fatty, amino acid, and mineral composition) of pork frankfurters enriched with 3% of yellow and white C. vulgaris. The inclusion of these microalgae resulted in a significant (P < 0.05) increase in protein (0.8- 0.85%) and ash, while moisture and fat content decreased. Following current Regulation (EC) No 1924/2006 and the amended Commission Regulation (EU) No 1047/2012, the ˝high protein˝ content claims can be assumed for microalgae enriched frankfurters. Carbohydrates increased with microalgae addition due to the increase in fibers. The sum of total essential amino acids and essential/nonessential amino acid ratio was higher (P < 0.05) in groups containing microalgae. In addition, white C. vulgaris samples had better Lys:Arg ratio compared to the control and honey C. vulgaris groups. Frankfurters with microalgae had significantly more (P < 0.05) n-3 PUFAs, mainly alfa-linolenic, gamma-linolenic, and eicosatrienoic acid, along with significantly lower amounts (P < 0.05) of SFAs, mainly palmitic and stearic acid. C. vulgaris mutants resulted in increased total PUFA, lower PUFA/SFA ratio, n−6/n−3 ratios, lower atherogenic index (AI), and thrombogenicity index (IT) compared to reference frankfurters. C. vulgaris inclusion in formulations increased (P < 0.05) Na, K, Ca, P and Zn, but lowered Mn compared to control frankfurters. In addition, frankfurters fortified with white C. vulgaris resulted in higher iron content and lower copper content compared to the control. Moreover, microalgae enrichment lowered the Na/K ratio by 34.74% and 16.42%, respectively, resulting in a significantly better ratio closer to the recommended one. Using microalgae to create innovative meat products can bring potential health benefits to consumers due to the enhanced nutritional profile of these products.
C3  - AlgaEurope 2022 Conference, 13-15 December 2022, Rome
T1  - Nutritional properties of frankfurters with added chlorophyll-deficient Chlorella vulgaris mutants
SP  - 88
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2569
ER  - 

@conference{
author = "Bošković Cabrol, Marija and Glišić, Milica and Baltić, Milan Ž. and Jovanović, Dragoljub and Silađi, Čaba and Simunović, Stefan and Tomašević, Igor and Raymundo, Anabela",
year = "2022",
abstract = "Microalgae have been proven to be a promising functional and sustainable ingredient in different food matrices, including some meat products [1,2,3,4,5,6]. However, the coloring of meat products containing microalgae Spirulina maxima and Chlorella vulgaris in a dark green could lead to consumers` rejection of these products. To overcome the color barrier, we used white and yellow chlorophyll-deficient C. vulgaris mutants, commercially available (Honey Chlorella), in designing acceptable products. The study aimed to analyze the nutritional composition (chemical, fatty, amino acid, and mineral composition) of pork frankfurters enriched with 3% of yellow and white C. vulgaris. The inclusion of these microalgae resulted in a significant (P < 0.05) increase in protein (0.8- 0.85%) and ash, while moisture and fat content decreased. Following current Regulation (EC) No 1924/2006 and the amended Commission Regulation (EU) No 1047/2012, the ˝high protein˝ content claims can be assumed for microalgae enriched frankfurters. Carbohydrates increased with microalgae addition due to the increase in fibers. The sum of total essential amino acids and essential/nonessential amino acid ratio was higher (P < 0.05) in groups containing microalgae. In addition, white C. vulgaris samples had better Lys:Arg ratio compared to the control and honey C. vulgaris groups. Frankfurters with microalgae had significantly more (P < 0.05) n-3 PUFAs, mainly alfa-linolenic, gamma-linolenic, and eicosatrienoic acid, along with significantly lower amounts (P < 0.05) of SFAs, mainly palmitic and stearic acid. C. vulgaris mutants resulted in increased total PUFA, lower PUFA/SFA ratio, n−6/n−3 ratios, lower atherogenic index (AI), and thrombogenicity index (IT) compared to reference frankfurters. C. vulgaris inclusion in formulations increased (P < 0.05) Na, K, Ca, P and Zn, but lowered Mn compared to control frankfurters. In addition, frankfurters fortified with white C. vulgaris resulted in higher iron content and lower copper content compared to the control. Moreover, microalgae enrichment lowered the Na/K ratio by 34.74% and 16.42%, respectively, resulting in a significantly better ratio closer to the recommended one. Using microalgae to create innovative meat products can bring potential health benefits to consumers due to the enhanced nutritional profile of these products.",
journal = "AlgaEurope 2022 Conference, 13-15 December 2022, Rome",
title = "Nutritional properties of frankfurters with added chlorophyll-deficient Chlorella vulgaris mutants",
pages = "88-90",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2569"
}

Bošković Cabrol, M., Glišić, M., Baltić, M. Ž., Jovanović, D., Silađi, Č., Simunović, S., Tomašević, I.,& Raymundo, A.. (2022). Nutritional properties of frankfurters with added chlorophyll-deficient Chlorella vulgaris mutants. in AlgaEurope 2022 Conference, 13-15 December 2022, Rome, 88-90.
https://hdl.handle.net/21.15107/rcub_veterinar_2569

Bošković Cabrol M, Glišić M, Baltić MŽ, Jovanović D, Silađi Č, Simunović S, Tomašević I, Raymundo A. Nutritional properties of frankfurters with added chlorophyll-deficient Chlorella vulgaris mutants. in AlgaEurope 2022 Conference, 13-15 December 2022, Rome. 2022;:88-90.
https://hdl.handle.net/21.15107/rcub_veterinar_2569 .

Bošković Cabrol, Marija, Glišić, Milica, Baltić, Milan Ž., Jovanović, Dragoljub, Silađi, Čaba, Simunović, Stefan, Tomašević, Igor, Raymundo, Anabela, "Nutritional properties of frankfurters with added chlorophyll-deficient Chlorella vulgaris mutants" in AlgaEurope 2022 Conference, 13-15 December 2022, Rome (2022):88-90,
https://hdl.handle.net/21.15107/rcub_veterinar_2569 .