TY  - JOUR
AU  - Ristanić, Marko
AU  - Zorc, Minja
AU  - Glavinić, Uroš
AU  - Stevanović, Jevrosima
AU  - Blagojević, Jovan
AU  - Maletić, Milan
AU  - Stanimirović, Zoran
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3706
AB  - To improve the genomic evaluation of milk-related traits in Holstein-Friesian (HF) cattle it
is essential to identify the associated candidate genes. Novel SNP-based analyses, such as the genetic
mapping of inherited diseases, GWAS, and genomic selection, have led to a new era of research.
The aim of this study was to analyze the association of each individual SNP in Serbian HF cattle
with milk production traits and inbreeding levels. The SNP 60 K chip Axiom Bovine BovMDv3
was deployed for the genotyping of 334 HF cows. The obtained genomic results, together with the
collected phenotypic data, were used for a GWAS. Moreover, the identification of ROH segments
was performed and served for inbreeding coefficient evaluation and ROH island detection. Using a
GWAS, a polymorphism, rs110619097 (located in the intron of the CTNNA3 gene), was detected to be
significantly (p < 0.01) associated with the milk protein concentration in the first lactation (adjusted to
305 days). The average genomic inbreeding value (FROH) was 0.079. ROH islands were discovered
in proximity to genes associated with milk production traits and genomic regions under selection
pressure for other economically important traits of dairy cattle. The findings of this pilot study
provide useful information for a better understanding of the genetic architecture of milk production
traits in Serbian HF dairy cows and can be used to improve lactation performances in Serbian HF
cattle breeding programs.
PB  - MDPI
T2  - Animals
T1  - Genome-wide analysis of milk production traits and selection signatures in Serbian Holstein-Friesian Cattle
VL  - 14
IS  - 5
SP  - 669
DO  - 10.3390/ani14050669
ER  - 

@article{
author = "Ristanić, Marko and Zorc, Minja and Glavinić, Uroš and Stevanović, Jevrosima and Blagojević, Jovan and Maletić, Milan and Stanimirović, Zoran",
year = "2024",
abstract = "To improve the genomic evaluation of milk-related traits in Holstein-Friesian (HF) cattle it
is essential to identify the associated candidate genes. Novel SNP-based analyses, such as the genetic
mapping of inherited diseases, GWAS, and genomic selection, have led to a new era of research.
The aim of this study was to analyze the association of each individual SNP in Serbian HF cattle
with milk production traits and inbreeding levels. The SNP 60 K chip Axiom Bovine BovMDv3
was deployed for the genotyping of 334 HF cows. The obtained genomic results, together with the
collected phenotypic data, were used for a GWAS. Moreover, the identification of ROH segments
was performed and served for inbreeding coefficient evaluation and ROH island detection. Using a
GWAS, a polymorphism, rs110619097 (located in the intron of the CTNNA3 gene), was detected to be
significantly (p < 0.01) associated with the milk protein concentration in the first lactation (adjusted to
305 days). The average genomic inbreeding value (FROH) was 0.079. ROH islands were discovered
in proximity to genes associated with milk production traits and genomic regions under selection
pressure for other economically important traits of dairy cattle. The findings of this pilot study
provide useful information for a better understanding of the genetic architecture of milk production
traits in Serbian HF dairy cows and can be used to improve lactation performances in Serbian HF
cattle breeding programs.",
publisher = "MDPI",
journal = "Animals",
title = "Genome-wide analysis of milk production traits and selection signatures in Serbian Holstein-Friesian Cattle",
volume = "14",
number = "5",
pages = "669",
doi = "10.3390/ani14050669"
}

Ristanić, M., Zorc, M., Glavinić, U., Stevanović, J., Blagojević, J., Maletić, M.,& Stanimirović, Z.. (2024). Genome-wide analysis of milk production traits and selection signatures in Serbian Holstein-Friesian Cattle. in Animals
MDPI., 14(5), 669.
https://doi.org/10.3390/ani14050669

Ristanić M, Zorc M, Glavinić U, Stevanović J, Blagojević J, Maletić M, Stanimirović Z. Genome-wide analysis of milk production traits and selection signatures in Serbian Holstein-Friesian Cattle. in Animals. 2024;14(5):669.
doi:10.3390/ani14050669 .

Ristanić, Marko, Zorc, Minja, Glavinić, Uroš, Stevanović, Jevrosima, Blagojević, Jovan, Maletić, Milan, Stanimirović, Zoran, "Genome-wide analysis of milk production traits and selection signatures in Serbian Holstein-Friesian Cattle" in Animals, 14, no. 5 (2024):669,
https://doi.org/10.3390/ani14050669 . .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Džogović, Danica
AU  - Jelisić, Stefan
AU  - Ristanić, Marko
AU  - Zorc, Minja
AU  - Aleksić, Nevenka
AU  - Stanimirović, Zoran
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2953
AB  - Nosema ceranae, a microsporidium species, is among the most common causes of bee diseases. The positive effect of Agaricus bisporus mushroom extract on the survival and immunity of Nosema-infected bees has been reported recently. The effect could be achieved by stimulating the expression of immune-related genes, but also by suppressing nosemosis. The aim of this work was to determine the effect of A. bisporus extract on the oxidative status of bees infected with N. ceranae. In a cage experiment on newly hatched bees, the effect of aqueous extract of champignon (A. bisporus, strain A15) was investigated. Six groups were formed: three groups were infected and received A. bisporus extract through food at different times (days 1, 3, and 6 after hatching), one group received the extract but was not infected (treatment control), one was only infected with Nosema (positive control) and one was neither infected nor received the extract (negative control). The effects were examined on samples taken on days 7 and 15 of the study. The activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) and the concentrations of malondialdehyde (MDA) were determined. In comparison to the positive control, the enzyme activities and MDA concentrations were significantly lower in the groups fed with the mushroom extract supplement. In the negative control, the level of oxidative stress was lower than in the positive control. In comparison with the other groups, the values mostly did not differ significantly. The oxidative status of bees infected with N. ceranae was significantly better if they were fed with the A. bisporus extract.
PB  - University of Belgrade, Faculty of Veterinary Medicine
T2  - Veterinarski Glasnik
T1  - Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract
VL  - 77
IS  - 1
SP  - 35
EP  - 50
DO  - 10.2298/VETGL220715013G
ER  - 

@article{
author = "Glavinić, Uroš and Džogović, Danica and Jelisić, Stefan and Ristanić, Marko and Zorc, Minja and Aleksić, Nevenka and Stanimirović, Zoran",
year = "2023",
abstract = "Nosema ceranae, a microsporidium species, is among the most common causes of bee diseases. The positive effect of Agaricus bisporus mushroom extract on the survival and immunity of Nosema-infected bees has been reported recently. The effect could be achieved by stimulating the expression of immune-related genes, but also by suppressing nosemosis. The aim of this work was to determine the effect of A. bisporus extract on the oxidative status of bees infected with N. ceranae. In a cage experiment on newly hatched bees, the effect of aqueous extract of champignon (A. bisporus, strain A15) was investigated. Six groups were formed: three groups were infected and received A. bisporus extract through food at different times (days 1, 3, and 6 after hatching), one group received the extract but was not infected (treatment control), one was only infected with Nosema (positive control) and one was neither infected nor received the extract (negative control). The effects were examined on samples taken on days 7 and 15 of the study. The activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD) and glutathione S-transferase (GST) and the concentrations of malondialdehyde (MDA) were determined. In comparison to the positive control, the enzyme activities and MDA concentrations were significantly lower in the groups fed with the mushroom extract supplement. In the negative control, the level of oxidative stress was lower than in the positive control. In comparison with the other groups, the values mostly did not differ significantly. The oxidative status of bees infected with N. ceranae was significantly better if they were fed with the A. bisporus extract.",
publisher = "University of Belgrade, Faculty of Veterinary Medicine",
journal = "Veterinarski Glasnik",
title = "Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract",
volume = "77",
number = "1",
pages = "35-50",
doi = "10.2298/VETGL220715013G"
}

Glavinić, U., Džogović, D., Jelisić, S., Ristanić, M., Zorc, M., Aleksić, N.,& Stanimirović, Z.. (2023). Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract. in Veterinarski Glasnik
University of Belgrade, Faculty of Veterinary Medicine., 77(1), 35-50.
https://doi.org/10.2298/VETGL220715013G

Glavinić U, Džogović D, Jelisić S, Ristanić M, Zorc M, Aleksić N, Stanimirović Z. Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract. in Veterinarski Glasnik. 2023;77(1):35-50.
doi:10.2298/VETGL220715013G .

Glavinić, Uroš, Džogović, Danica, Jelisić, Stefan, Ristanić, Marko, Zorc, Minja, Aleksić, Nevenka, Stanimirović, Zoran, "Oxidative status of honey bees infected with Nosema ceranae microsporidium and supplemented with Agaricus bisporus mushroom extract" in Veterinarski Glasnik, 77, no. 1 (2023):35-50,
https://doi.org/10.2298/VETGL220715013G . .

TY  - JOUR
AU  - Tesovnik, Tanja
AU  - Zorc, Minja
AU  - Ristanić, Marko
AU  - Glavinić, Uroš
AU  - Stevanović, Jevrosima
AU  - Narat, Mojca
AU  - Stanimirović, Zoran
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1798
AB  - During their lifetime honey bees (Apis mellifera) rarely experience optimal conditions. Sometimes, a simultaneous action of multiple stressors, natural and chemical, results in even greater effect than of any stressor alone. Therefore, integrative investigations of different factors affecting honey bees have to be carried out. In this study, adult honey bees exposed to thiamethoxam in larval and/or adult stage and infected with Nosema ceranae were examined. Newly emerged bees from colonies, non-treated or treated with thiamethoxam, were organized in six groups and kept in cages. Thiamethoxam treated bees were further exposed to either thiamethoxam or Nosema (groups TT and TN), or simultaneously to both (group TTN). Newly emerged bees from non-treated colonies were exposed to Nosema (group CN). From both, treated and non-treated colonies two groups were organized and further fed only with sugar solution (groups C and TC). Here, we present the expression profile of 19 genes in adult worker honey bees comprising those involved in immune, detoxification, development and apoptosis response. Results showed that gene expression patterns changed with time and depended on the treatment. In group TC at the time of emergence the majority of tested genes were downregulated, among which nine were significantly altered. The same gene pattern was observed on day six, where the only significantly upregulated gene was defensin-1. On day nine most of analyzed genes in all experimental groups showed upregulation compared to control group, where upregulation of antimicrobial peptide genes abaecin, defensin-1 and defensin-2 was significant in groups TT and TTN. On day 15 we observed a similar pattern of expression in groups TC and TT exposed to thiamethoxam only, where most of the detoxification genes were downregulated. Additionally RNA loads of Nosema and honey bee viruses were recorded. We detected a synergistic interaction of thiamethoxam and Nosema, reflected in lowest honey bee survival.
PB  - Elsevier Sci Ltd, Oxford
T2  - Environmental Pollution
T1  - Exposure of honey bee larvae to thiamethoxam and its interaction with Nosema ceranae infection in adult honey bees
VL  - 256
SP  - 113443
DO  - 10.1016/j.envpol.2019.113443
ER  - 

@article{
author = "Tesovnik, Tanja and Zorc, Minja and Ristanić, Marko and Glavinić, Uroš and Stevanović, Jevrosima and Narat, Mojca and Stanimirović, Zoran",
year = "2020",
abstract = "During their lifetime honey bees (Apis mellifera) rarely experience optimal conditions. Sometimes, a simultaneous action of multiple stressors, natural and chemical, results in even greater effect than of any stressor alone. Therefore, integrative investigations of different factors affecting honey bees have to be carried out. In this study, adult honey bees exposed to thiamethoxam in larval and/or adult stage and infected with Nosema ceranae were examined. Newly emerged bees from colonies, non-treated or treated with thiamethoxam, were organized in six groups and kept in cages. Thiamethoxam treated bees were further exposed to either thiamethoxam or Nosema (groups TT and TN), or simultaneously to both (group TTN). Newly emerged bees from non-treated colonies were exposed to Nosema (group CN). From both, treated and non-treated colonies two groups were organized and further fed only with sugar solution (groups C and TC). Here, we present the expression profile of 19 genes in adult worker honey bees comprising those involved in immune, detoxification, development and apoptosis response. Results showed that gene expression patterns changed with time and depended on the treatment. In group TC at the time of emergence the majority of tested genes were downregulated, among which nine were significantly altered. The same gene pattern was observed on day six, where the only significantly upregulated gene was defensin-1. On day nine most of analyzed genes in all experimental groups showed upregulation compared to control group, where upregulation of antimicrobial peptide genes abaecin, defensin-1 and defensin-2 was significant in groups TT and TTN. On day 15 we observed a similar pattern of expression in groups TC and TT exposed to thiamethoxam only, where most of the detoxification genes were downregulated. Additionally RNA loads of Nosema and honey bee viruses were recorded. We detected a synergistic interaction of thiamethoxam and Nosema, reflected in lowest honey bee survival.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Environmental Pollution",
title = "Exposure of honey bee larvae to thiamethoxam and its interaction with Nosema ceranae infection in adult honey bees",
volume = "256",
pages = "113443",
doi = "10.1016/j.envpol.2019.113443"
}

Tesovnik, T., Zorc, M., Ristanić, M., Glavinić, U., Stevanović, J., Narat, M.,& Stanimirović, Z.. (2020). Exposure of honey bee larvae to thiamethoxam and its interaction with Nosema ceranae infection in adult honey bees. in Environmental Pollution
Elsevier Sci Ltd, Oxford., 256, 113443.
https://doi.org/10.1016/j.envpol.2019.113443

Tesovnik T, Zorc M, Ristanić M, Glavinić U, Stevanović J, Narat M, Stanimirović Z. Exposure of honey bee larvae to thiamethoxam and its interaction with Nosema ceranae infection in adult honey bees. in Environmental Pollution. 2020;256:113443.
doi:10.1016/j.envpol.2019.113443 .

Tesovnik, Tanja, Zorc, Minja, Ristanić, Marko, Glavinić, Uroš, Stevanović, Jevrosima, Narat, Mojca, Stanimirović, Zoran, "Exposure of honey bee larvae to thiamethoxam and its interaction with Nosema ceranae infection in adult honey bees" in Environmental Pollution, 256 (2020):113443,
https://doi.org/10.1016/j.envpol.2019.113443 . .

TY  - JOUR
AU  - Glavinić, Uroš
AU  - Tesovnik, Tanja
AU  - Stevanović, Jevrosima
AU  - Zorc, Minja
AU  - Cizelj, Ivanka
AU  - Stanimirović, Zoran
AU  - Narat, Mojca
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1739
AB  - Among numerous factors that contribute to honey bee colony losses and problems in beekeeping, pesticides and Nosema ceranae have been often reported. In contrast to insecticides, whose effects on bees have been widely studied, fungicides did not attract considerable attention. Prochloraz, an imidazole fungicide widely used in agriculture, was detected in honey and pollen stored inside hives and has been already proven to alter immune gene expression of honey bees at different developmental stages. The aim of this study was to simulate the realistic conditions of migratory beekeeping, where colonies, both uninfected and infected with N. ceranae, are frequently transported to the vicinity of crop fields treated with prochloraz. We investigated the combined effect of prochloraz and N. ceranae on honey bees that faced fungicide during the larval stage through food consumption and microsporidium infection afterwards. The most pronounced changes in gene expression were observed in newly emerged Nosema-free bees originating from colonies previously contaminated with prochloraz. As exclusively upregulation was registered, prochloraz alone most likely acts as a challenge that induces activation of immune pathways in newly emerged bees. The combination of both stressors (prochloraz and Nosema infection) exerted the greatest effect on sixday-old honey bees. Among ten genes with significantly altered expression, half were upregulated and half downregulated. N. ceranae as a sole stressor had the weakest effects on immune gene expression modulation with only three genes significantly dysregulated. In conclusion, food contaminated with prochloraz consumed in larval stage could present a threat to the development of immunity and detoxification mechanisms in honey bees.
PB  - Peerj Inc, London
T2  - PEERJ
T1  - Response of adult honey bees treated in larval stage with prochloraz to infection with Nosema ceranae
VL  - 7
SP  - e6325
DO  - 10.7717/peerj.6325
ER  - 

@article{
author = "Glavinić, Uroš and Tesovnik, Tanja and Stevanović, Jevrosima and Zorc, Minja and Cizelj, Ivanka and Stanimirović, Zoran and Narat, Mojca",
year = "2019",
abstract = "Among numerous factors that contribute to honey bee colony losses and problems in beekeeping, pesticides and Nosema ceranae have been often reported. In contrast to insecticides, whose effects on bees have been widely studied, fungicides did not attract considerable attention. Prochloraz, an imidazole fungicide widely used in agriculture, was detected in honey and pollen stored inside hives and has been already proven to alter immune gene expression of honey bees at different developmental stages. The aim of this study was to simulate the realistic conditions of migratory beekeeping, where colonies, both uninfected and infected with N. ceranae, are frequently transported to the vicinity of crop fields treated with prochloraz. We investigated the combined effect of prochloraz and N. ceranae on honey bees that faced fungicide during the larval stage through food consumption and microsporidium infection afterwards. The most pronounced changes in gene expression were observed in newly emerged Nosema-free bees originating from colonies previously contaminated with prochloraz. As exclusively upregulation was registered, prochloraz alone most likely acts as a challenge that induces activation of immune pathways in newly emerged bees. The combination of both stressors (prochloraz and Nosema infection) exerted the greatest effect on sixday-old honey bees. Among ten genes with significantly altered expression, half were upregulated and half downregulated. N. ceranae as a sole stressor had the weakest effects on immune gene expression modulation with only three genes significantly dysregulated. In conclusion, food contaminated with prochloraz consumed in larval stage could present a threat to the development of immunity and detoxification mechanisms in honey bees.",
publisher = "Peerj Inc, London",
journal = "PEERJ",
title = "Response of adult honey bees treated in larval stage with prochloraz to infection with Nosema ceranae",
volume = "7",
pages = "e6325",
doi = "10.7717/peerj.6325"
}

Glavinić, U., Tesovnik, T., Stevanović, J., Zorc, M., Cizelj, I., Stanimirović, Z.,& Narat, M.. (2019). Response of adult honey bees treated in larval stage with prochloraz to infection with Nosema ceranae. in PEERJ
Peerj Inc, London., 7, e6325.
https://doi.org/10.7717/peerj.6325

Glavinić U, Tesovnik T, Stevanović J, Zorc M, Cizelj I, Stanimirović Z, Narat M. Response of adult honey bees treated in larval stage with prochloraz to infection with Nosema ceranae. in PEERJ. 2019;7:e6325.
doi:10.7717/peerj.6325 .

Glavinić, Uroš, Tesovnik, Tanja, Stevanović, Jevrosima, Zorc, Minja, Cizelj, Ivanka, Stanimirović, Zoran, Narat, Mojca, "Response of adult honey bees treated in larval stage with prochloraz to infection with Nosema ceranae" in PEERJ, 7 (2019):e6325,
https://doi.org/10.7717/peerj.6325 . .