@article{
author = "Bajagić, Bojana and Mrkun, Janko and Kirovski, Danijela and Savić, Đorđe and Budimir, Draženko and Maletić, Jelena and Maletić, Milan",
year = "2021",
abstract = "diagnoses and their accuracy to predict pregnancy. The study was performed on 51
postpartum cross breed dairy cows with pathological puerperium. Artificial insemination
(AI) was done in spontaneous estrus. Endometrium samples were taken by cytotape
(CT), cervical mucus (CM) samples by Metricheck and smears for crystallization degree
were obtained at AI day. Blood serum samples for progesterone (P4) concentration
determination were taken at AI day and 17 days later. Cows were examined by
ultrasound 35 days after AI to confirm pregnancy. Percent (%) of polymorphnuclear
leukocytes (PMNs) taken by CT were expressed by ROC curve (with cut-off point set
at 1%). Out of the total of 51 cows involved in the study, 13.73% true positive and
41.17% true negative cows were found (54.90%). The content of CM sampled with
Metricheck was altered in 11 cows (21.57%), and one of them was pregnant (1.96%).
An average macroscopic CM value for pregnant cows was 0.06±0.24 vs. 0.39±0.79
for non-pregnant (p<0.05). An average value of CM crystallization in pregnant cows
was 2.50±1.04 vs. 1.85±1.15 in non-pregnant (p<0.05). P4 concentration was not
statistically different at AI day, but was statistically significantly higher 17 days later
in pregnant cows (p = 0.002). CT alterations may be a reliable diagnostic tool for
SCE diagnosis, while macroscopic CM alterations may be used for CE detection. P4
determination is beneficial in pregnancy diagnosis while CM crystallization degree is
helpful in determination of best time for AI., Cilj studije bio je procena različitih metoda za dijagnostiku supkliničkog endometritisa
(SKE) i njihove pouzdanosti za predviđanje graviditeta. U studiju je bila uključena 51
postpartalna mlečna krava mešane rase sa patološkim puerperijem. Veštačko osemenjavanje
(VO) rađeno je u spontanom estrusu. Na dan VO uzeti su uzorci endometrijuma
dobijeni cytotape (CT) tehnikom, kao i uzorci cervikalne sluzi (CS) dobijeni
upotrebom Metričeka. Razmaz CS, urađen isti dan, korišten je za ispitivanje stepena
kristalizacije. Uzorci krvi za određivanje koncentracije progesterona (P4) uzeti su na
dan VO i 17 dana kasnije. Krave su pregledane ultrazvukom 35 dana nakon VO radi
potvrđivanja graviditeta. Procenat (%) polimorfonuklearnih leukocita (PMNs) dobijenih CT tehnikom je izražen ROC krivom (granična vrednost je određena na 1%).
Od ukupno 54,90% krava uključenih u studiju, utvrđeno je 13,73% stvarno pozitivnih
i 41,17% stvarno negativnih krava. Izmenjen sadržaj CS uzorkovane Metričekom
utvrđen je kod jedanaest krava (21,57%), ali samo jedna (1,96%) je bila gravidna. Prosečna
makroskopska vrednost CS kod gravidnih krava je bila 0,06±0,24 u odnosu na
0,39±0,79 kod negravidnih (p<0,05). Prosečna vrednost kristalizacije CS kod gravidnih
krava je bila 2,50±1,04 u odnosu na 1,85±1,15 kod negravidnih (p<0,05). Koncentracija
P4 nije se statistički razlikovala na dan VO, ali je bila statistički značajno
veća 17 dana kasnije kod gravidnih životinja (p = 0.002). Promene utvrđene CT mogu
biti indikator SKE, a promene CS dobijene Metričekom mogu biti indikator kliničkog
endometritisa. Određivanje P4 korisno je u dijagnostici graviditeta, a kristalizacija CS
može se koristiti kao pomoćni metod za određivanje najpovoljnijeg vremena za VO.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria-Beograd",
title = "Assessment of different diagnostic methods for the identification of subclinical endometritis in dairy cows with pathological puerperium and their reliability to conceive, Procena različitih dijagnostičkih metoda za identifikaciju supkliničkog endometritisa mlečnih krava sa patološkim puerperijumom i njihova pouzdanost u predikciji graviditeta",
volume = "71",
number = "4",
pages = "462-476",
doi = "10.2478/acve-2021-0038"
}
