Vidaković Knežević, Suzana

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  • Vidaković Knežević, Suzana (2)
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Author's Bibliography

Effects of selected essential oils on Listeria monocytogenes in biofilms and in a model food system

Vidaković Knežević, Suzana; Knežević, Slobodan; Vranešević, Jelena; Kravić, Snežana Ž.; Lakićević, Brankica; Kocić-Tanackov, Sunčica; Karabasil, Nedjeljko

(MDPI, 2023)

TY  - JOUR
AU  - Vidaković Knežević, Suzana
AU  - Knežević, Slobodan
AU  - Vranešević, Jelena
AU  - Kravić, Snežana Ž.
AU  - Lakićević, Brankica
AU  - Kocić-Tanackov, Sunčica
AU  - Karabasil, Nedjeljko
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2940
AB  - The composition of 18 essential oils was determined using gas chromatography–mass spectrometry, and their antilisterial activity was evaluated by the disk diffusion method, followed by the determination of the minimum inhibitory and minimum bactericidal concentrations. The most active essential oils were oregano, thyme, cinnamon, winter savory, and clove, with MIC values ranging from 0.09 to 1.78 µL/mL. We investigated the biofilm-forming potential of Listeria monocytogenes on polystyrene at 5 °C, 15 °C, and 37 °C in three different media. The formation of biofilm was found to be dependent on the temperature and the availability of nutrients. After treatment with selected essential oils, the reduction in biofilm biomass was in the range of 32.61% and 78.62%. Micromorphological changes in the L. monocytogenes treated by oregano and thyme essential oils were observed in the form of impaired cell integrity and cell lyses by using scanning electron microscope. Oregano and thyme essential oils (MIC and 2MIC) significantly (p < 0.05) reduced the population of L. monocytogenes in minced pork meat during storage at 4 °C. In conclusion, the obtained results indicated the good activity of some selected essential oils on L. monocytogenes, with bacteriostatic, bactericidal, and antibiofilm effects at very low concentrations.
PB  - MDPI
T2  - Foods
T1  - Effects of selected essential oils on Listeria monocytogenes in biofilms and in a model food system
VL  - 12
IS  - 10
SP  - 1930
DO  - 10.3390/foods12101930
ER  - 
@article{
author = "Vidaković Knežević, Suzana and Knežević, Slobodan and Vranešević, Jelena and Kravić, Snežana Ž. and Lakićević, Brankica and Kocić-Tanackov, Sunčica and Karabasil, Nedjeljko",
year = "2023",
abstract = "The composition of 18 essential oils was determined using gas chromatography–mass spectrometry, and their antilisterial activity was evaluated by the disk diffusion method, followed by the determination of the minimum inhibitory and minimum bactericidal concentrations. The most active essential oils were oregano, thyme, cinnamon, winter savory, and clove, with MIC values ranging from 0.09 to 1.78 µL/mL. We investigated the biofilm-forming potential of Listeria monocytogenes on polystyrene at 5 °C, 15 °C, and 37 °C in three different media. The formation of biofilm was found to be dependent on the temperature and the availability of nutrients. After treatment with selected essential oils, the reduction in biofilm biomass was in the range of 32.61% and 78.62%. Micromorphological changes in the L. monocytogenes treated by oregano and thyme essential oils were observed in the form of impaired cell integrity and cell lyses by using scanning electron microscope. Oregano and thyme essential oils (MIC and 2MIC) significantly (p < 0.05) reduced the population of L. monocytogenes in minced pork meat during storage at 4 °C. In conclusion, the obtained results indicated the good activity of some selected essential oils on L. monocytogenes, with bacteriostatic, bactericidal, and antibiofilm effects at very low concentrations.",
publisher = "MDPI",
journal = "Foods",
title = "Effects of selected essential oils on Listeria monocytogenes in biofilms and in a model food system",
volume = "12",
number = "10",
pages = "1930",
doi = "10.3390/foods12101930"
}
Vidaković Knežević, S., Knežević, S., Vranešević, J., Kravić, S. Ž., Lakićević, B., Kocić-Tanackov, S.,& Karabasil, N.. (2023). Effects of selected essential oils on Listeria monocytogenes in biofilms and in a model food system. in Foods
MDPI., 12(10), 1930.
https://doi.org/10.3390/foods12101930
Vidaković Knežević S, Knežević S, Vranešević J, Kravić SŽ, Lakićević B, Kocić-Tanackov S, Karabasil N. Effects of selected essential oils on Listeria monocytogenes in biofilms and in a model food system. in Foods. 2023;12(10):1930.
doi:10.3390/foods12101930 .
Vidaković Knežević, Suzana, Knežević, Slobodan, Vranešević, Jelena, Kravić, Snežana Ž., Lakićević, Brankica, Kocić-Tanackov, Sunčica, Karabasil, Nedjeljko, "Effects of selected essential oils on Listeria monocytogenes in biofilms and in a model food system" in Foods, 12, no. 10 (2023):1930,
https://doi.org/10.3390/foods12101930 . .
1
3

Dobra uzorkivačka praksa – preduslov pouzdanog pregleda mesa na prisustvo larvi Trichinella spp. u mesu divljih svinja

Vranešević, Jelena; Vidaković Knežević, Suzana; Pelić, Miloš; Aleksić Radojković, Jelena; Plavša, Nada; Ljubojević Pelić, Dragana

(Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze, 2022)

TY  - CONF
AU  - Vranešević, Jelena
AU  - Vidaković Knežević, Suzana
AU  - Pelić, Miloš
AU  - Aleksić Radojković, Jelena
AU  - Plavša, Nada
AU  - Ljubojević Pelić, Dragana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2801
AB  - Дивље свиње могу бити резервоар инфекције Trichinellа spp. за домаће
животиње, али и директан извор трихинелозе људи. У највећем броју држава
Европе, трихинелоза се ретко јавља, док се на подручју некадашње Југославије
(изузев Македоније и Словеније) јавља у виду спорадичних или масовних
епидемија сваке године због чега се територије Србије, Босне и Херцеговине и
Хрватске сматрају ендемским подручјем трихинелозе. Законом је прописан, у
свим земљама региона, обавезан преглед меса дивљих животиња које су
намењене за конзумирање. У нашој лабораторији се акредитованом методом
„Микробиологија ланца хране – Откривање ларви Trichinella у месу методом
вештачке дигестије“ (SRPS EN ISO 18743:2016) годишње прегледа преко 200
узорака меса дивљих свиња. Приликом пријема узорaка у лабораторију се
дешавало да узорци буду неадекватни (≈ 10%), самим тим поуздан преглед меса
на присуство Trichinella spp. није било могуће спровести. Како би се у што већој
мери смањио број неадекватних узорака, наша лабораторија је издала „Упутство
за узорковање, транспорт и складиштење узорака дивљих свиња за испитивање
присуства ларви Trichinella spp. - метода вештачке дигестије“ које је достављено
ловачким удружењима који достављају узорке меса дивљих свиња нашој
лабораторији. Након тога, проценат неадекватних узорака се сваке године
смањује, па је тако за 2018. годину он износио 3,14%, за 2019. годину 1,85%, за
2020. годину 0,42%. У 2021. години ниједан пристигао узорак у нашу
лабораторију није био неадекватан.
AB  - Wild boars could be a reservoir of Trichinella spp. for domestic animals, but also a
direct source of human trichinelosis. In most European countries, trichinelosis is rare,
while in the area of the former Yugoslavia (except Macedonia and Slovenia) it occurs
in the form of sporadic or mass epidemics every year, which is why the territories of
Serbia, Bosnia and Herzegovina and Croatia are considered endemic to trichinelosis.
The law prescribes, in all countries of the region, a mandatory inspection of the meat
of wild animals that are intended for consumption. In our laboratory, over 200 samples
of wild boar meat are examined annually with the accredited method "Microbiology
of the food chain - Detection of Trichinella larvae in meat by artificial digestion"
(SRPS EN ISO 18743: 2016). When receiving samples in the laboratory, it happened
that the samples were inadequate (≈ 10%), thus a reliable finding for the presence of
Trichinella spp. was not possible. In order to reduce the number of inadequate samples
as much as possible, our laboratory has issued "Instruction for sampling, transport and
storage of wild boar samples for testing the presence of Trichinella spp. - method of
artificial digestion” which was delivered to hunting societies which deliver samples
of wild boar meat to our laboratory. After that, the percentage of inadequate samples
decreases every year, so in 2018 it was 3.14%, in 2019 1.85%, in 2020 0.42%. In
2021, not a single sample arrived at our laboratory was inadequate.
PB  - Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze
C3  - XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022.
T1  - Dobra uzorkivačka praksa – preduslov pouzdanog pregleda mesa na prisustvo larvi Trichinella spp. u mesu divljih svinja
T1  - Good sampling practice-prerequisite of a reliable examination of presence of Trichinella spp. in the wild boar meat
SP  - 124
EP  - 125
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2801
ER  - 
@conference{
author = "Vranešević, Jelena and Vidaković Knežević, Suzana and Pelić, Miloš and Aleksić Radojković, Jelena and Plavša, Nada and Ljubojević Pelić, Dragana",
year = "2022",
abstract = "Дивље свиње могу бити резервоар инфекције Trichinellа spp. за домаће
животиње, али и директан извор трихинелозе људи. У највећем броју држава
Европе, трихинелоза се ретко јавља, док се на подручју некадашње Југославије
(изузев Македоније и Словеније) јавља у виду спорадичних или масовних
епидемија сваке године због чега се територије Србије, Босне и Херцеговине и
Хрватске сматрају ендемским подручјем трихинелозе. Законом је прописан, у
свим земљама региона, обавезан преглед меса дивљих животиња које су
намењене за конзумирање. У нашој лабораторији се акредитованом методом
„Микробиологија ланца хране – Откривање ларви Trichinella у месу методом
вештачке дигестије“ (SRPS EN ISO 18743:2016) годишње прегледа преко 200
узорака меса дивљих свиња. Приликом пријема узорaка у лабораторију се
дешавало да узорци буду неадекватни (≈ 10%), самим тим поуздан преглед меса
на присуство Trichinella spp. није било могуће спровести. Како би се у што већој
мери смањио број неадекватних узорака, наша лабораторија је издала „Упутство
за узорковање, транспорт и складиштење узорака дивљих свиња за испитивање
присуства ларви Trichinella spp. - метода вештачке дигестије“ које је достављено
ловачким удружењима који достављају узорке меса дивљих свиња нашој
лабораторији. Након тога, проценат неадекватних узорака се сваке године
смањује, па је тако за 2018. годину он износио 3,14%, за 2019. годину 1,85%, за
2020. годину 0,42%. У 2021. години ниједан пристигао узорак у нашу
лабораторију није био неадекватан., Wild boars could be a reservoir of Trichinella spp. for domestic animals, but also a
direct source of human trichinelosis. In most European countries, trichinelosis is rare,
while in the area of the former Yugoslavia (except Macedonia and Slovenia) it occurs
in the form of sporadic or mass epidemics every year, which is why the territories of
Serbia, Bosnia and Herzegovina and Croatia are considered endemic to trichinelosis.
The law prescribes, in all countries of the region, a mandatory inspection of the meat
of wild animals that are intended for consumption. In our laboratory, over 200 samples
of wild boar meat are examined annually with the accredited method "Microbiology
of the food chain - Detection of Trichinella larvae in meat by artificial digestion"
(SRPS EN ISO 18743: 2016). When receiving samples in the laboratory, it happened
that the samples were inadequate (≈ 10%), thus a reliable finding for the presence of
Trichinella spp. was not possible. In order to reduce the number of inadequate samples
as much as possible, our laboratory has issued "Instruction for sampling, transport and
storage of wild boar samples for testing the presence of Trichinella spp. - method of
artificial digestion” which was delivered to hunting societies which deliver samples
of wild boar meat to our laboratory. After that, the percentage of inadequate samples
decreases every year, so in 2018 it was 3.14%, in 2019 1.85%, in 2020 0.42%. In
2021, not a single sample arrived at our laboratory was inadequate.",
publisher = "Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze",
journal = "XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022.",
title = "Dobra uzorkivačka praksa – preduslov pouzdanog pregleda mesa na prisustvo larvi Trichinella spp. u mesu divljih svinja, Good sampling practice-prerequisite of a reliable examination of presence of Trichinella spp. in the wild boar meat",
pages = "124-125",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2801"
}
Vranešević, J., Vidaković Knežević, S., Pelić, M., Aleksić Radojković, J., Plavša, N.,& Ljubojević Pelić, D.. (2022). Dobra uzorkivačka praksa – preduslov pouzdanog pregleda mesa na prisustvo larvi Trichinella spp. u mesu divljih svinja. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022.
Beograd : Srpsko veterinarsko društvo, Sekcija za zoonoze., 124-125.
https://hdl.handle.net/21.15107/rcub_veterinar_2801
Vranešević J, Vidaković Knežević S, Pelić M, Aleksić Radojković J, Plavša N, Ljubojević Pelić D. Dobra uzorkivačka praksa – preduslov pouzdanog pregleda mesa na prisustvo larvi Trichinella spp. u mesu divljih svinja. in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022.. 2022;:124-125.
https://hdl.handle.net/21.15107/rcub_veterinar_2801 .
Vranešević, Jelena, Vidaković Knežević, Suzana, Pelić, Miloš, Aleksić Radojković, Jelena, Plavša, Nada, Ljubojević Pelić, Dragana, "Dobra uzorkivačka praksa – preduslov pouzdanog pregleda mesa na prisustvo larvi Trichinella spp. u mesu divljih svinja" in XXIV Simpozijum epizootiologa i epidemiologa (XXIV Epizootiološki dani), Subotica, 27 - 29. april 2022. (2022):124-125,
https://hdl.handle.net/21.15107/rcub_veterinar_2801 .