TY  - JOUR
AU  - Šarić, Ljubiša
AU  - Premović, Tamara
AU  - Šarić, Bojana
AU  - Čabarkapa, Ivana
AU  - Todorić, Olja
AU  - Miljanić, Jelena
AU  - Lazarević, Jasmina
AU  - Karabasil, Nedjeljko
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2556
AB  - The aim of this study was to examine the microbiological quality of raw donkey milk of an indigenous Serbian breed as well as the changes in the microbial populations during storage at 4 °C. In addition, antibacterial activity of donkey milk against E. coli, L. monocytogenes and S. aureus at 15 °C as well as the content of the two main antibacterial proteins lysozyme and lactoferrin were investigated. Microbiological examination of 137 individual milk samples collected over a period of 21 months showed good microbiological quality since foodborne pathogens such as Salmonella spp. and L. monocytogenes were not detected in any of the analyzed samples, while the number of E. coli, Enterobacteriaceae, total coliform bacteria, sulfite-reducing Clostridia and aerobic sporogenic bacteria was below the limit of quantification (<1 cfu mL−1). During the six-days storage at 4 °C, total bacterial counts and the counts of lactic acid bacteria remained at the initial level while pathogenic bacteria were not detected. The strongest antibacterial activity of the tested milk was observed against E. coli, while S. aureus was the least sensitive to milk antibacterial compounds. Although further research is needed to fully elucidate the antibacterial mechanism and synergistic activity of different compounds in donkey milk, the high content lysozyme (2.63 ± 0.03 g L−1) and lactoferrin (15.48 mg L−1) observed in tested milk could contribute to its strong antibacterial activity and extension of the storage period during which it can be safely consumed.
PB  - MDPI
T2  - Animals
T1  - Microbiological Quality of Raw Donkey Milk from Serbia and Its Antibacterial Properties at Pre-Cooling Temperature
VL  - 13
IS  - 3
SP  - 327
DO  - 10.3390/ani13030327
ER  - 

@article{
author = "Šarić, Ljubiša and Premović, Tamara and Šarić, Bojana and Čabarkapa, Ivana and Todorić, Olja and Miljanić, Jelena and Lazarević, Jasmina and Karabasil, Nedjeljko",
year = "2023",
abstract = "The aim of this study was to examine the microbiological quality of raw donkey milk of an indigenous Serbian breed as well as the changes in the microbial populations during storage at 4 °C. In addition, antibacterial activity of donkey milk against E. coli, L. monocytogenes and S. aureus at 15 °C as well as the content of the two main antibacterial proteins lysozyme and lactoferrin were investigated. Microbiological examination of 137 individual milk samples collected over a period of 21 months showed good microbiological quality since foodborne pathogens such as Salmonella spp. and L. monocytogenes were not detected in any of the analyzed samples, while the number of E. coli, Enterobacteriaceae, total coliform bacteria, sulfite-reducing Clostridia and aerobic sporogenic bacteria was below the limit of quantification (<1 cfu mL−1). During the six-days storage at 4 °C, total bacterial counts and the counts of lactic acid bacteria remained at the initial level while pathogenic bacteria were not detected. The strongest antibacterial activity of the tested milk was observed against E. coli, while S. aureus was the least sensitive to milk antibacterial compounds. Although further research is needed to fully elucidate the antibacterial mechanism and synergistic activity of different compounds in donkey milk, the high content lysozyme (2.63 ± 0.03 g L−1) and lactoferrin (15.48 mg L−1) observed in tested milk could contribute to its strong antibacterial activity and extension of the storage period during which it can be safely consumed.",
publisher = "MDPI",
journal = "Animals",
title = "Microbiological Quality of Raw Donkey Milk from Serbia and Its Antibacterial Properties at Pre-Cooling Temperature",
volume = "13",
number = "3",
pages = "327",
doi = "10.3390/ani13030327"
}

Šarić, L., Premović, T., Šarić, B., Čabarkapa, I., Todorić, O., Miljanić, J., Lazarević, J.,& Karabasil, N.. (2023). Microbiological Quality of Raw Donkey Milk from Serbia and Its Antibacterial Properties at Pre-Cooling Temperature. in Animals
MDPI., 13(3), 327.
https://doi.org/10.3390/ani13030327

Šarić L, Premović T, Šarić B, Čabarkapa I, Todorić O, Miljanić J, Lazarević J, Karabasil N. Microbiological Quality of Raw Donkey Milk from Serbia and Its Antibacterial Properties at Pre-Cooling Temperature. in Animals. 2023;13(3):327.
doi:10.3390/ani13030327 .

Šarić, Ljubiša, Premović, Tamara, Šarić, Bojana, Čabarkapa, Ivana, Todorić, Olja, Miljanić, Jelena, Lazarević, Jasmina, Karabasil, Nedjeljko, "Microbiological Quality of Raw Donkey Milk from Serbia and Its Antibacterial Properties at Pre-Cooling Temperature" in Animals, 13, no. 3 (2023):327,
https://doi.org/10.3390/ani13030327 . .