TY  - CONF
AU  - Maletić, Milan
AU  - Blagojević, Jovan
AU  - Vakanjac, Slobodanka
AU  - Stanišić, Ljubodrag
AU  - Magaš, Vladimir
AU  - Đurić, Miloje
AU  - Radinović, Miodrag
AU  - Đorđević, Milan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3278
AB  - Fiziologija mlečnih krava kao i mnogi egzogeni i endogeni faktori utiču na
ekspresiju spoljašnjih znakova estrusa, a uz loš menadžment stada i neobučeno osoblje stopa detekcije estrusa je vrlo često ispod 50%. Ekonomska održivost
proizvodnje u direktnoj je korelaciji sa reproduktivnom efikasnošću. Skraćenje
međutelidbenog intervala uz optimalnu proizvodnju mleka je prioritet na farmama
mlečnih krava. Pravovremena detekcija estrusa je preduslov dobre reprodukcije,
pri čemu primena hormonskih protokola sa fiksnim osemenjavanjem predstavlja
dobru alternativu detekciji estrusa. Na velikim farmskim sistemima u intenzivnoj
proizvodnji mlečnih krava gde je uočavanje estrusa otežano, uvođenje protokola za sinhronizaciju estrusa i ovulacije, kao i protokola resinhronizacije za drugo i
ostala osemenjavanja omogućavaju dobro upravljanje stadom, smanjenja indeksa osemenjavanja, skraćenja međutelidbenog intervala i racionalno poslovanje
farme. Sa tim u vezi u ovom radu predstavićemo najčešće korišćene protokole resinhronizacije estrusa i ovulacije i njihovu uspešnost kod krava koje su predhodno
jednom ili više puta osemenjene.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023
T1  - Uspešnost primene protokola resinhronizacije estrusa i ovulacije kod krava
SP  - 21
EP  - 30
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3278
ER  - 

@conference{
author = "Maletić, Milan and Blagojević, Jovan and Vakanjac, Slobodanka and Stanišić, Ljubodrag and Magaš, Vladimir and Đurić, Miloje and Radinović, Miodrag and Đorđević, Milan",
year = "2023",
abstract = "Fiziologija mlečnih krava kao i mnogi egzogeni i endogeni faktori utiču na
ekspresiju spoljašnjih znakova estrusa, a uz loš menadžment stada i neobučeno osoblje stopa detekcije estrusa je vrlo često ispod 50%. Ekonomska održivost
proizvodnje u direktnoj je korelaciji sa reproduktivnom efikasnošću. Skraćenje
međutelidbenog intervala uz optimalnu proizvodnju mleka je prioritet na farmama
mlečnih krava. Pravovremena detekcija estrusa je preduslov dobre reprodukcije,
pri čemu primena hormonskih protokola sa fiksnim osemenjavanjem predstavlja
dobru alternativu detekciji estrusa. Na velikim farmskim sistemima u intenzivnoj
proizvodnji mlečnih krava gde je uočavanje estrusa otežano, uvođenje protokola za sinhronizaciju estrusa i ovulacije, kao i protokola resinhronizacije za drugo i
ostala osemenjavanja omogućavaju dobro upravljanje stadom, smanjenja indeksa osemenjavanja, skraćenja međutelidbenog intervala i racionalno poslovanje
farme. Sa tim u vezi u ovom radu predstavićemo najčešće korišćene protokole resinhronizacije estrusa i ovulacije i njihovu uspešnost kod krava koje su predhodno
jednom ili više puta osemenjene.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023",
title = "Uspešnost primene protokola resinhronizacije estrusa i ovulacije kod krava",
pages = "21-30",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3278"
}

Maletić, M., Blagojević, J., Vakanjac, S., Stanišić, L., Magaš, V., Đurić, M., Radinović, M.,& Đorđević, M.. (2023). Uspešnost primene protokola resinhronizacije estrusa i ovulacije kod krava. in 14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 21-30.
https://hdl.handle.net/21.15107/rcub_veterinar_3278

Maletić M, Blagojević J, Vakanjac S, Stanišić L, Magaš V, Đurić M, Radinović M, Đorđević M. Uspešnost primene protokola resinhronizacije estrusa i ovulacije kod krava. in 14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023. 2023;:21-30.
https://hdl.handle.net/21.15107/rcub_veterinar_3278 .

Maletić, Milan, Blagojević, Jovan, Vakanjac, Slobodanka, Stanišić, Ljubodrag, Magaš, Vladimir, Đurić, Miloje, Radinović, Miodrag, Đorđević, Milan, "Uspešnost primene protokola resinhronizacije estrusa i ovulacije kod krava" in 14. Naučni simpozijum Reprodukcija životinja, Divčibare, 12 - 15. oktobar 2023 (2023):21-30,
https://hdl.handle.net/21.15107/rcub_veterinar_3278 .

TY  - JOUR
AU  - Tomanić, Dragana
AU  - Božin, Biljana
AU  - Čabarkapa, Ivana
AU  - Kladar, Nebojša
AU  - Radinović, Miodrag
AU  - Maletić, Milan
AU  - Kovačević, Zorana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2458
AB  - Mastitis is one of the most common and costly diseases affecting dairy cows worldwide. Since antibiotic resistance has become a global threat to both animal and human health, it is becoming more urgent to continuously search for new therapeutical alternatives for the control and treatment of bovine mastitis. Hence, our research aimed to test the therapeutic use of two essential oils (EOs) based on their chemical composition, antibacterial and antioxidant potential. The present study was conducted by collecting milk samples from the cows diagnosed with clinical or subclinical mastitis with the aim of isolating and identifying bacterial strains. The antioxidant potential of essential oils of Menthae piperitae (MP) and Melissa officinalis (MO) was evaluated in several in vitro assays. In the MP EO, a total of 38 compounds were identified, with menthol as the dominant compound, whereas in MO EO 51 compounds were identified. Furthermore, the values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) have been used to quantitatively measure the antibacterial activity of each essential oil. In accordance with which, MP EO samples exhibited a higher degree of antibacterial activity than MO EO. Thus, EOs have been shown to be promising alternatives to antibiotics because of their  availability,  biodegradability,  and lower risk of  side effects as compared with conventional, antimicrobial treatment. Nevertheless,  further  clinical  studies  are  needed  to  test  the  potential  role  of   EOs  in  treating mastitis in dairy cows.
PB  - Vaculty of Veterinary Medicine, University of Belgrade
T2  - Acta Veterinaria
T1  - Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens
T1  - Hemijski sastav, antioksidativni potencijal i antibakterijska aktivnost dva različita etarska ulja protiv uzročnika mastitisa
VL  - 72
IS  - 1
SP  - 45
EP  - 58
DO  - 10.2478/acve-2022-0004
ER  - 

@article{
author = "Tomanić, Dragana and Božin, Biljana and Čabarkapa, Ivana and Kladar, Nebojša and Radinović, Miodrag and Maletić, Milan and Kovačević, Zorana",
year = "2022",
abstract = "Mastitis is one of the most common and costly diseases affecting dairy cows worldwide. Since antibiotic resistance has become a global threat to both animal and human health, it is becoming more urgent to continuously search for new therapeutical alternatives for the control and treatment of bovine mastitis. Hence, our research aimed to test the therapeutic use of two essential oils (EOs) based on their chemical composition, antibacterial and antioxidant potential. The present study was conducted by collecting milk samples from the cows diagnosed with clinical or subclinical mastitis with the aim of isolating and identifying bacterial strains. The antioxidant potential of essential oils of Menthae piperitae (MP) and Melissa officinalis (MO) was evaluated in several in vitro assays. In the MP EO, a total of 38 compounds were identified, with menthol as the dominant compound, whereas in MO EO 51 compounds were identified. Furthermore, the values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) have been used to quantitatively measure the antibacterial activity of each essential oil. In accordance with which, MP EO samples exhibited a higher degree of antibacterial activity than MO EO. Thus, EOs have been shown to be promising alternatives to antibiotics because of their  availability,  biodegradability,  and lower risk of  side effects as compared with conventional, antimicrobial treatment. Nevertheless,  further  clinical  studies  are  needed  to  test  the  potential  role  of   EOs  in  treating mastitis in dairy cows.",
publisher = "Vaculty of Veterinary Medicine, University of Belgrade",
journal = "Acta Veterinaria",
title = "Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens, Hemijski sastav, antioksidativni potencijal i antibakterijska aktivnost dva različita etarska ulja protiv uzročnika mastitisa",
volume = "72",
number = "1",
pages = "45-58",
doi = "10.2478/acve-2022-0004"
}

Tomanić, D., Božin, B., Čabarkapa, I., Kladar, N., Radinović, M., Maletić, M.,& Kovačević, Z.. (2022). Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens. in Acta Veterinaria
Vaculty of Veterinary Medicine, University of Belgrade., 72(1), 45-58.
https://doi.org/10.2478/acve-2022-0004

Tomanić D, Božin B, Čabarkapa I, Kladar N, Radinović M, Maletić M, Kovačević Z. Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens. in Acta Veterinaria. 2022;72(1):45-58.
doi:10.2478/acve-2022-0004 .

Tomanić, Dragana, Božin, Biljana, Čabarkapa, Ivana, Kladar, Nebojša, Radinović, Miodrag, Maletić, Milan, Kovačević, Zorana, "Chemical composition, antioxidant and antibacterial activity of two different essential oils against mastitis associated pathogens" in Acta Veterinaria, 72, no. 1 (2022):45-58,
https://doi.org/10.2478/acve-2022-0004 . .

TY  - JOUR
AU  - Đorđević, Milan
AU  - Blagojević, Jovan
AU  - Radinović, Miodrag
AU  - Cincović, Marko
AU  - Maletić, Jelena
AU  - Kreszinger, Mario
AU  - Maletić, Milan
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2832
AB  - The reproductive efficiency of the cows was monitored after two resynchronization protocols: Ovsynch (OVS) and Double Ovsynch (DOS). The research initially included 70 HF cows who entered the first synchronization protocol - Presynch. Cows that did not conceive after the first synchronization were divided into two groups and introduced to two resynchronization protocols. In the first group of cows (n=35), the DOS protocol began with the application of GnRH on day 22 after the Presynch TAI (Timed Artificial Insemination), and seven days later pregnancy check-up was done and PGF2α was applied only to non-pregnant cows (n=23), which remained in the study. In the second group of cows, the OVS protocol started on day 32 after Presynch TAI only in non-pregnant animals (n=20). Progesterone (P4) concentration was determined at the time of application of GnRH1, PGF2α and GnRH2 in both groups of cows, and then 30 days after Resynch TAI, ultrasound pregnancy diagnosis was done. A higher percentage of pregnant cows were recorded in the OVS group compared to the DOS group (45% and 35%, respectively). The concentration of P4 in the serum of cows in the DOS group during the first measurement (GnRH1) was significantly higher than the value in cows that did not conceive (p<0.05), while in the third measurement (GnRH2) the average concentration of P4 in conceiving cows was significantly lower (p<0.001) compared to cows that did not coincive. The open days period was significantly longer in pregnant cows that were resynchronized using the DOS protocol compared to cows from the OVS protocol. In conclusion, the OVS protocol of estrus resynchronization in dairy cows proved to be more successful than the DOS protocol. However, considering the advantages the OVS, it is needed to determine which day of the sexual cycle is the best to start resynchronization.
PB  - Sciendo
T2  - Acta Veterinaria
T1  - Efficiency of two protocols of resynchronization of estrus and ovulation in high-producing dairy cows at peak lactation
VL  - 72
VL  - 3
SP  - 362
SP  - 374
DO  - 10.2478/acve-2022-0029
ER  - 

@article{
author = "Đorđević, Milan and Blagojević, Jovan and Radinović, Miodrag and Cincović, Marko and Maletić, Jelena and Kreszinger, Mario and Maletić, Milan",
year = "2022",
abstract = "The reproductive efficiency of the cows was monitored after two resynchronization protocols: Ovsynch (OVS) and Double Ovsynch (DOS). The research initially included 70 HF cows who entered the first synchronization protocol - Presynch. Cows that did not conceive after the first synchronization were divided into two groups and introduced to two resynchronization protocols. In the first group of cows (n=35), the DOS protocol began with the application of GnRH on day 22 after the Presynch TAI (Timed Artificial Insemination), and seven days later pregnancy check-up was done and PGF2α was applied only to non-pregnant cows (n=23), which remained in the study. In the second group of cows, the OVS protocol started on day 32 after Presynch TAI only in non-pregnant animals (n=20). Progesterone (P4) concentration was determined at the time of application of GnRH1, PGF2α and GnRH2 in both groups of cows, and then 30 days after Resynch TAI, ultrasound pregnancy diagnosis was done. A higher percentage of pregnant cows were recorded in the OVS group compared to the DOS group (45% and 35%, respectively). The concentration of P4 in the serum of cows in the DOS group during the first measurement (GnRH1) was significantly higher than the value in cows that did not conceive (p<0.05), while in the third measurement (GnRH2) the average concentration of P4 in conceiving cows was significantly lower (p<0.001) compared to cows that did not coincive. The open days period was significantly longer in pregnant cows that were resynchronized using the DOS protocol compared to cows from the OVS protocol. In conclusion, the OVS protocol of estrus resynchronization in dairy cows proved to be more successful than the DOS protocol. However, considering the advantages the OVS, it is needed to determine which day of the sexual cycle is the best to start resynchronization.",
publisher = "Sciendo",
journal = "Acta Veterinaria",
title = "Efficiency of two protocols of resynchronization of estrus and ovulation in high-producing dairy cows at peak lactation",
volume = "72, 3",
pages = "362-374",
doi = "10.2478/acve-2022-0029"
}

Đorđević, M., Blagojević, J., Radinović, M., Cincović, M., Maletić, J., Kreszinger, M.,& Maletić, M.. (2022). Efficiency of two protocols of resynchronization of estrus and ovulation in high-producing dairy cows at peak lactation. in Acta Veterinaria
Sciendo., 72, 362.
https://doi.org/10.2478/acve-2022-0029

Đorđević M, Blagojević J, Radinović M, Cincović M, Maletić J, Kreszinger M, Maletić M. Efficiency of two protocols of resynchronization of estrus and ovulation in high-producing dairy cows at peak lactation. in Acta Veterinaria. 2022;72:362.
doi:10.2478/acve-2022-0029 .

Đorđević, Milan, Blagojević, Jovan, Radinović, Miodrag, Cincović, Marko, Maletić, Jelena, Kreszinger, Mario, Maletić, Milan, "Efficiency of two protocols of resynchronization of estrus and ovulation in high-producing dairy cows at peak lactation" in Acta Veterinaria, 72 (2022):362,
https://doi.org/10.2478/acve-2022-0029 . .

TY  - JOUR
AU  - Kovačević, Zorana
AU  - Kladar, Nebojša
AU  - Čabarkapa, Ivana
AU  - Radinović, Miodrag
AU  - Maletić, Milan
AU  - Erdeljan, Mihajlo
AU  - Božin, Biljana
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2390
AB  - Mastitis represents a heavy burden for the dairy sector worldwide with high economic and animal welfare impact. Antibiotic treatment is an important component of mastitis control programs. However, emergence and transfer of antimicrobial-resistant (AMR) bacteria is becoming a growing concern. Therefore, the development of novel agents is required for prevention and treatment of mastitis. Hence, our aim was to assess the antibacterial properties of two essential oils (EOs) obtained from oregano (Origanum vulgare L., Lamiaceae) and mountain savory (Satureja montana L., Lamiaceae) against mastitis-associated bacteria in Serbia. The chemical composition and antioxidant potential of these EOs were also evaluated. The present study was conducted on strains derived from aseptic milk samples collected from Holstein-Friesian cows with clinical or subclinical mastitis, during the morning milking. Clinical mastitis was assessed by clinical examination, while subclinical mastitis was confirmed using somatic cell count in the milk samples. The microdilution method was used to determine the antibacterial activity, while antioxidant potential of the EOs was evaluated in several in vitro assays. The values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were used to quantitatively measure the antibacterial activity of each EO. MIC/MBC ranged from 0.78/6.25 and 0.39/0.78 mg/mL for oregano and mountain savory, respectively. A total of 25 compounds were identified in the oregano EO, while 47 were identified in winter savory EO, among which aromatic oxygenated monoterpenes were the most abundant compounds. The tested EOs have shown promising antimicrobial activity and could be considered as one of the treatment approaches in mastitis-affected cows.
PB  - MDPI
T2  - Antibiotics
T1  - New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives
VL  - 10
IS  - 12
DO  - 10.3390/antibiotics10121460
ER  - 

@article{
author = "Kovačević, Zorana and Kladar, Nebojša and Čabarkapa, Ivana and Radinović, Miodrag and Maletić, Milan and Erdeljan, Mihajlo and Božin, Biljana",
year = "2021",
abstract = "Mastitis represents a heavy burden for the dairy sector worldwide with high economic and animal welfare impact. Antibiotic treatment is an important component of mastitis control programs. However, emergence and transfer of antimicrobial-resistant (AMR) bacteria is becoming a growing concern. Therefore, the development of novel agents is required for prevention and treatment of mastitis. Hence, our aim was to assess the antibacterial properties of two essential oils (EOs) obtained from oregano (Origanum vulgare L., Lamiaceae) and mountain savory (Satureja montana L., Lamiaceae) against mastitis-associated bacteria in Serbia. The chemical composition and antioxidant potential of these EOs were also evaluated. The present study was conducted on strains derived from aseptic milk samples collected from Holstein-Friesian cows with clinical or subclinical mastitis, during the morning milking. Clinical mastitis was assessed by clinical examination, while subclinical mastitis was confirmed using somatic cell count in the milk samples. The microdilution method was used to determine the antibacterial activity, while antioxidant potential of the EOs was evaluated in several in vitro assays. The values of minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) were used to quantitatively measure the antibacterial activity of each EO. MIC/MBC ranged from 0.78/6.25 and 0.39/0.78 mg/mL for oregano and mountain savory, respectively. A total of 25 compounds were identified in the oregano EO, while 47 were identified in winter savory EO, among which aromatic oxygenated monoterpenes were the most abundant compounds. The tested EOs have shown promising antimicrobial activity and could be considered as one of the treatment approaches in mastitis-affected cows.",
publisher = "MDPI",
journal = "Antibiotics",
title = "New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives",
volume = "10",
number = "12",
doi = "10.3390/antibiotics10121460"
}

Kovačević, Z., Kladar, N., Čabarkapa, I., Radinović, M., Maletić, M., Erdeljan, M.,& Božin, B.. (2021). New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives. in Antibiotics
MDPI., 10(12).
https://doi.org/10.3390/antibiotics10121460

Kovačević Z, Kladar N, Čabarkapa I, Radinović M, Maletić M, Erdeljan M, Božin B. New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives. in Antibiotics. 2021;10(12).
doi:10.3390/antibiotics10121460 .

Kovačević, Zorana, Kladar, Nebojša, Čabarkapa, Ivana, Radinović, Miodrag, Maletić, Milan, Erdeljan, Mihajlo, Božin, Biljana, "New Perspective of Origanum vulgare L. and Satureja montana L. Essential Oils as Bovine Mastitis Treatment Alternatives" in Antibiotics, 10, no. 12 (2021),
https://doi.org/10.3390/antibiotics10121460 . .

TY  - JOUR
AU  - Babić, Milijana
AU  - Pajić, Marija
AU  - Radinović, Miodrag
AU  - Boboš, Stanko
AU  - Bulajić, Snežana
AU  - Nikolić, Aleksandra
AU  - Velebit, Branko
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1784
AB  - The aim of this study was to determine the effects of different temperatures and storage time on Staphylococcus aureus growth, sea gene expression, and synthesis of staphylococcal enterotoxin A (SEA) in the pasteurized and UHT-pasteurized milk. Pasteurized and UHT-pasteurized milk were inoculated with 3.98 log(10) CFU/mL of S. aureus (ATCC 13565). Inoculated milk samples were stored at 8 degrees C, 15 degrees C, and 22 degrees C for 24, 48, and 72 h, respectively. SEA synthesis was detected with a fully automated miniVIDAS instrument using the Enzyme-Linked Fluorescent Assay (ELFA) technology. The patterns of gene regulation were detected by quantitative reverse transcriptase PCR. The 2(-Delta Delta CT) method has been used as a relative quantification strategy for gene expression responses data analysis. The results indicated that growth rate, sea gene expression, and SEA synthesis were influenced by type of milk, storage time, and temperature. Incubation of milk at different temperatures (15 degrees C and 22 degrees C) and times was used to simulate inadequate transport and storage conditions. Storage of pasteurized milk at 22 degrees C for 24 h significantly upregulated the expression of sea gene compared with milk stored at 8 degrees C, which coincides with the achieved S. aureus number of 10(5) CFU/mL and detected amount of SEA. In addition, storage of UHT-pasteurized milk at 22 degrees C for 24 h and at 15 degrees C for 48 h significantly upregulated the sea gene expression compared with milk stored at 8 degrees C, which coincides with the detected amount of SEA and the dynamics of S. aureus number change. It can, therefore, be concluded that implementing good hygiene practices to avoid pre- and post-heat treatment milk contamination and maintaining the cold chain at temperature <8 degrees C throughout the entire dairy production chain are of paramount importance to decrease the risk of staphylococcal food poisoning.
PB  - Mary Ann Liebert, Inc, New Rochelle
T2  - Foodborne Pathogens and Disease
T1  - Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk
VL  - 16
IS  - 4
SP  - 282
EP  - 289
DO  - 10.1089/fpd.2018.2544
ER  - 

@article{
author = "Babić, Milijana and Pajić, Marija and Radinović, Miodrag and Boboš, Stanko and Bulajić, Snežana and Nikolić, Aleksandra and Velebit, Branko",
year = "2019",
abstract = "The aim of this study was to determine the effects of different temperatures and storage time on Staphylococcus aureus growth, sea gene expression, and synthesis of staphylococcal enterotoxin A (SEA) in the pasteurized and UHT-pasteurized milk. Pasteurized and UHT-pasteurized milk were inoculated with 3.98 log(10) CFU/mL of S. aureus (ATCC 13565). Inoculated milk samples were stored at 8 degrees C, 15 degrees C, and 22 degrees C for 24, 48, and 72 h, respectively. SEA synthesis was detected with a fully automated miniVIDAS instrument using the Enzyme-Linked Fluorescent Assay (ELFA) technology. The patterns of gene regulation were detected by quantitative reverse transcriptase PCR. The 2(-Delta Delta CT) method has been used as a relative quantification strategy for gene expression responses data analysis. The results indicated that growth rate, sea gene expression, and SEA synthesis were influenced by type of milk, storage time, and temperature. Incubation of milk at different temperatures (15 degrees C and 22 degrees C) and times was used to simulate inadequate transport and storage conditions. Storage of pasteurized milk at 22 degrees C for 24 h significantly upregulated the expression of sea gene compared with milk stored at 8 degrees C, which coincides with the achieved S. aureus number of 10(5) CFU/mL and detected amount of SEA. In addition, storage of UHT-pasteurized milk at 22 degrees C for 24 h and at 15 degrees C for 48 h significantly upregulated the sea gene expression compared with milk stored at 8 degrees C, which coincides with the detected amount of SEA and the dynamics of S. aureus number change. It can, therefore, be concluded that implementing good hygiene practices to avoid pre- and post-heat treatment milk contamination and maintaining the cold chain at temperature <8 degrees C throughout the entire dairy production chain are of paramount importance to decrease the risk of staphylococcal food poisoning.",
publisher = "Mary Ann Liebert, Inc, New Rochelle",
journal = "Foodborne Pathogens and Disease",
title = "Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk",
volume = "16",
number = "4",
pages = "282-289",
doi = "10.1089/fpd.2018.2544"
}

Babić, M., Pajić, M., Radinović, M., Boboš, S., Bulajić, S., Nikolić, A.,& Velebit, B.. (2019). Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk. in Foodborne Pathogens and Disease
Mary Ann Liebert, Inc, New Rochelle., 16(4), 282-289.
https://doi.org/10.1089/fpd.2018.2544

Babić M, Pajić M, Radinović M, Boboš S, Bulajić S, Nikolić A, Velebit B. Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk. in Foodborne Pathogens and Disease. 2019;16(4):282-289.
doi:10.1089/fpd.2018.2544 .

Babić, Milijana, Pajić, Marija, Radinović, Miodrag, Boboš, Stanko, Bulajić, Snežana, Nikolić, Aleksandra, Velebit, Branko, "Effects of Temperature Abuse on the Growth and Staphylococcal Enterotoxin A Gene (sea) Expression of Staphylococcus aureus in Milk" in Foodborne Pathogens and Disease, 16, no. 4 (2019):282-289,
https://doi.org/10.1089/fpd.2018.2544 . .

TY  - JOUR
AU  - Pajić, Marija
AU  - Boboš, Stanko
AU  - Velebit, Branko
AU  - Rašić, Zoran
AU  - Katić, Vera
AU  - Radinović, Miodrag
AU  - Nikolić, Aleksandra
AU  - Simonović, Dušan
AU  - Babić, Milijana
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1355
AB  - Staphylococcus aureus is known worldwide as a frequent cause of mastitis in dairy cattle. Due to the production of heath resistant enterotoxins, this pathogen is also a major cause of food poisoning among humans, with symptoms of often severe vomiting and diarrhea. The aim of our study was to determine the prevalence of enterotoxinproducing strains of S. aureus originating from samples of cows with subclinical and clinical mastitis in the Republic of Serbia. Furthermore, we analyzed the type of staphylococcal enterotoxin they produce and phylogenetic relatedness among the S. aureus isolates recovered from milk in this study. Production of staphylococcal enterotoxins A, B, C, D and E was determined by commercial immunoenzyme assay VIDAS® SET2, and presence of corresponding genes encoding enterotoxin synthesis in positive isolates confirmed by Polymerase Chain Reaction. Enterotoxin production was determined in 5 out of 75 (6.67%) isolates of S. aureus and all of them produced staphylococcal enterotoxins C. After analyzing the nucleotide sequence of the gene encoding the synthesis of staphylococcal protein A, S. aureus isolates were assigned into 2 phylogenetic groups, including 7 clusters. All S. aureus isolates with the presence of sec gene formed one cluster even dough they originated from milk samples from different farms.
AB  - Širom sveta S. aureus poznat je kao čest uzročnik mastitisa krava. Takođe predstavlja i glavni uzrok trovanja hranom nakon konzumiranja hrane kontaminirane njegovim enterotoksinima. Cilj ovog istraživanja bio je da se ispita prevalencija enterotoksinprodukujućih sojeva S. aureus poreklom iz vimena krava sa subkliničkim i kliničkim mastitisom u Srbiji, da se odredi tip enterotoksina koji produkuju i da se odredi filogenetska srodnost ovih izolata. Za određivanje sposobnosti sinteze stafilokoknih enterotoksina A, B, C, D i E korišćen je VIDAS® SET2 immunoenzimski test i utvrđeno je da 5 od 75 (6,67%) izolata S. aureus sintetiše enterotoksine. Lančanom reakcijom polimeraze na prisustvo odgovarajućih gena, utvrđeno je da svih 5 izolata poseduju gen za sintezu enterotoksina C. Analizom nukleotidnih sekvenci gena za sintezu stafilokoknog proteina A, izolati S. aureus grupisani su filogenetski u 2 grupe, odnosno u 7 klastera. Svi izolati S. aureus kod kojih je dokazano prisustvo gena za sintezu enterotoksina spadaju u isti klaster.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows
T1  - Prevalencija i molekularna karakterizacija enterotoksin-produkujućih sojeva S. aureus izolovanih iz vimena krava u Srbiji
VL  - 66
IS  - 4
SP  - 466
EP  - 477
DO  - 10.1515/acve-2016-0040
ER  - 

@article{
author = "Pajić, Marija and Boboš, Stanko and Velebit, Branko and Rašić, Zoran and Katić, Vera and Radinović, Miodrag and Nikolić, Aleksandra and Simonović, Dušan and Babić, Milijana",
year = "2016",
abstract = "Staphylococcus aureus is known worldwide as a frequent cause of mastitis in dairy cattle. Due to the production of heath resistant enterotoxins, this pathogen is also a major cause of food poisoning among humans, with symptoms of often severe vomiting and diarrhea. The aim of our study was to determine the prevalence of enterotoxinproducing strains of S. aureus originating from samples of cows with subclinical and clinical mastitis in the Republic of Serbia. Furthermore, we analyzed the type of staphylococcal enterotoxin they produce and phylogenetic relatedness among the S. aureus isolates recovered from milk in this study. Production of staphylococcal enterotoxins A, B, C, D and E was determined by commercial immunoenzyme assay VIDAS® SET2, and presence of corresponding genes encoding enterotoxin synthesis in positive isolates confirmed by Polymerase Chain Reaction. Enterotoxin production was determined in 5 out of 75 (6.67%) isolates of S. aureus and all of them produced staphylococcal enterotoxins C. After analyzing the nucleotide sequence of the gene encoding the synthesis of staphylococcal protein A, S. aureus isolates were assigned into 2 phylogenetic groups, including 7 clusters. All S. aureus isolates with the presence of sec gene formed one cluster even dough they originated from milk samples from different farms., Širom sveta S. aureus poznat je kao čest uzročnik mastitisa krava. Takođe predstavlja i glavni uzrok trovanja hranom nakon konzumiranja hrane kontaminirane njegovim enterotoksinima. Cilj ovog istraživanja bio je da se ispita prevalencija enterotoksinprodukujućih sojeva S. aureus poreklom iz vimena krava sa subkliničkim i kliničkim mastitisom u Srbiji, da se odredi tip enterotoksina koji produkuju i da se odredi filogenetska srodnost ovih izolata. Za određivanje sposobnosti sinteze stafilokoknih enterotoksina A, B, C, D i E korišćen je VIDAS® SET2 immunoenzimski test i utvrđeno je da 5 od 75 (6,67%) izolata S. aureus sintetiše enterotoksine. Lančanom reakcijom polimeraze na prisustvo odgovarajućih gena, utvrđeno je da svih 5 izolata poseduju gen za sintezu enterotoksina C. Analizom nukleotidnih sekvenci gena za sintezu stafilokoknog proteina A, izolati S. aureus grupisani su filogenetski u 2 grupe, odnosno u 7 klastera. Svi izolati S. aureus kod kojih je dokazano prisustvo gena za sintezu enterotoksina spadaju u isti klaster.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows, Prevalencija i molekularna karakterizacija enterotoksin-produkujućih sojeva S. aureus izolovanih iz vimena krava u Srbiji",
volume = "66",
number = "4",
pages = "466-477",
doi = "10.1515/acve-2016-0040"
}

Pajić, M., Boboš, S., Velebit, B., Rašić, Z., Katić, V., Radinović, M., Nikolić, A., Simonović, D.,& Babić, M.. (2016). Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(4), 466-477.
https://doi.org/10.1515/acve-2016-0040

Pajić M, Boboš S, Velebit B, Rašić Z, Katić V, Radinović M, Nikolić A, Simonović D, Babić M. Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows. in Acta Veterinaria-Beograd. 2016;66(4):466-477.
doi:10.1515/acve-2016-0040 .

Pajić, Marija, Boboš, Stanko, Velebit, Branko, Rašić, Zoran, Katić, Vera, Radinović, Miodrag, Nikolić, Aleksandra, Simonović, Dušan, Babić, Milijana, "Prevalence and molecular characterization of enterotoxin-producing strains of Staphylococcus aureus isolated from Serbian dairy cows" in Acta Veterinaria-Beograd, 66, no. 4 (2016):466-477,
https://doi.org/10.1515/acve-2016-0040 . .