TY  - JOUR
AU  - Sukara, Ratko
AU  - Juwaid, Salem
AU  - Ćirović, Duško
AU  - Penezić, Aleksandra
AU  - Mihaljica, Darko
AU  - Veinović, Gorana
AU  - Radojičić, Sonja
AU  - Hodzić, Adnan
AU  - Duscher, Gerhard Georg
AU  - Tomanović, Snežana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1767
AB  - Human activities such as deforestation, urbanization, and environmental pollution lead to a reduction in the spatial boundary between wild animals, domestic animals and humans. These activities increase the risk for the emergence of pathogens from the sylvatic cycle in the population of domestic animals and humans. Foxes are recognized as potential reservoirs for a number of bacterial pathogens of medical and public health concern. The aim of the present study was to investigate the prevalence and spatial distribution of bacterial tick-borne pathogens from the Anaplasmataceae family, Borrelia burgdorferi sensu lato (s.l.), Rickettsia spp., Coxiella burnetii, Francisella tularensis, Bartonella spp., in the red fox population from Serbia and to discuss the obtained results from the epidemiological point of view. Legally hunted red foxes (Vulpes vulpes) from 14 localities in Serbia were included in the study and spleen samples from 129 animals were tested with conventional PCR assays for the presence of bacterial tick-borne pathogens. DNA of Candidatus Neoehrlichia sp. (FU98), Borrelia burgdorferi sensu stricto, Borrelia lusitaniae, and Borrelia garinii was detected in 6 (4.7%), 1 (0.8%), 2 (1.6%) and 1 (0.8%) animals, respectively. Co-infection by Candidatus Neoehrlichia sp. (FU98) and B. garinii was detected in one animal. All samples were negative for other tested bacterial tick-borne pathogens. The results of the present study indicate the potential role of foxes in natural cycles of Candidatus Neoehrlichia sp. (FU98) and causative agents of Lyme borreliosis in the investigated areas. Further research is required to elucidate the role of foxes in the epidemiology of these and other tick-borne zoonotic pathogens in the Republic of Serbia.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia
VL  - 69
IS  - 3
SP  - 312
EP  - 324
DO  - 10.2478/acve-2019-0026
ER  - 

@article{
author = "Sukara, Ratko and Juwaid, Salem and Ćirović, Duško and Penezić, Aleksandra and Mihaljica, Darko and Veinović, Gorana and Radojičić, Sonja and Hodzić, Adnan and Duscher, Gerhard Georg and Tomanović, Snežana",
year = "2019",
abstract = "Human activities such as deforestation, urbanization, and environmental pollution lead to a reduction in the spatial boundary between wild animals, domestic animals and humans. These activities increase the risk for the emergence of pathogens from the sylvatic cycle in the population of domestic animals and humans. Foxes are recognized as potential reservoirs for a number of bacterial pathogens of medical and public health concern. The aim of the present study was to investigate the prevalence and spatial distribution of bacterial tick-borne pathogens from the Anaplasmataceae family, Borrelia burgdorferi sensu lato (s.l.), Rickettsia spp., Coxiella burnetii, Francisella tularensis, Bartonella spp., in the red fox population from Serbia and to discuss the obtained results from the epidemiological point of view. Legally hunted red foxes (Vulpes vulpes) from 14 localities in Serbia were included in the study and spleen samples from 129 animals were tested with conventional PCR assays for the presence of bacterial tick-borne pathogens. DNA of Candidatus Neoehrlichia sp. (FU98), Borrelia burgdorferi sensu stricto, Borrelia lusitaniae, and Borrelia garinii was detected in 6 (4.7%), 1 (0.8%), 2 (1.6%) and 1 (0.8%) animals, respectively. Co-infection by Candidatus Neoehrlichia sp. (FU98) and B. garinii was detected in one animal. All samples were negative for other tested bacterial tick-borne pathogens. The results of the present study indicate the potential role of foxes in natural cycles of Candidatus Neoehrlichia sp. (FU98) and causative agents of Lyme borreliosis in the investigated areas. Further research is required to elucidate the role of foxes in the epidemiology of these and other tick-borne zoonotic pathogens in the Republic of Serbia.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia",
volume = "69",
number = "3",
pages = "312-324",
doi = "10.2478/acve-2019-0026"
}

Sukara, R., Juwaid, S., Ćirović, D., Penezić, A., Mihaljica, D., Veinović, G., Radojičić, S., Hodzić, A., Duscher, G. G.,& Tomanović, S.. (2019). Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3), 312-324.
https://doi.org/10.2478/acve-2019-0026

Sukara R, Juwaid S, Ćirović D, Penezić A, Mihaljica D, Veinović G, Radojičić S, Hodzić A, Duscher GG, Tomanović S. Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia. in Acta Veterinaria-Beograd. 2019;69(3):312-324.
doi:10.2478/acve-2019-0026 .

Sukara, Ratko, Juwaid, Salem, Ćirović, Duško, Penezić, Aleksandra, Mihaljica, Darko, Veinović, Gorana, Radojičić, Sonja, Hodzić, Adnan, Duscher, Gerhard Georg, Tomanović, Snežana, "Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia" in Acta Veterinaria-Beograd, 69, no. 3 (2019):312-324,
https://doi.org/10.2478/acve-2019-0026 . .

TY  - JOUR
AU  - Janjić, Filip
AU  - Sarvan, Darko
AU  - Tomanović, Snežana
AU  - Cuk, Jelena
AU  - Krstić, Vanja
AU  - Radonjić, Vladimir
AU  - Kovačević-Filipović, Milica
AU  - Ajtić, Jelena
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1783
AB  - Belgrade, the capital of the Republic of Serbia, is an endemic location for canine babesiosis caused by Babesia canis. This tick-borne disease occurs seasonally in regions with moderate continental climate. However, recent clinical data show that 1) some cases of canine babesiosis have been recorded during the winter, and 2) canine babesiosis is spreading to the northern parts of Europe with a colder climate, which is a region previously free of this disease. Our study investigates the occurrence of canine babesiosis in different seasons over 2013-2016, and explores a short-term link between meteorological parameters and number of diagnosed cases of canine babesiosis in Belgrade. It also looks into possible long-term correlations that span one year before the onset of the disease. Based on 872 recorded cases over four years, our results show a bimodal seasonal distribution of canine babesiosis, with a pronounced peak in the spring, and a less conspicuous one in the autumn. Throughout the year, even over the coldest and warmest periods, there is a broad range of temperatures and relative humidifies when the disease is recorded. Over one year prior to the spring and autumn onset of the disease, we found a noticeable impact of temperature and relative humidity, and to a lesser extent, of atmospheric pressure and cloud cover, on the number of diagnosed cases. These findings imply short-term and long-term relationships between occurrence of acute canine babesiosis and certain meteorological parameters, and they open further questions that need to be investigated in order to understand the epidemiology of this disease.
PB  - Elsevier Gmbh, Munich
T2  - Ticks and Tick-Borne Diseases
T1  - A short-term and long-term relationship between occurrence of acute canine babesiosis and meteorological parameters in Belgrade, Serbia
VL  - 10
IS  - 6
SP  - UNSP 101273
DO  - 10.1016/j.ttbdis.2019.101273
ER  - 

@article{
author = "Janjić, Filip and Sarvan, Darko and Tomanović, Snežana and Cuk, Jelena and Krstić, Vanja and Radonjić, Vladimir and Kovačević-Filipović, Milica and Ajtić, Jelena",
year = "2019",
abstract = "Belgrade, the capital of the Republic of Serbia, is an endemic location for canine babesiosis caused by Babesia canis. This tick-borne disease occurs seasonally in regions with moderate continental climate. However, recent clinical data show that 1) some cases of canine babesiosis have been recorded during the winter, and 2) canine babesiosis is spreading to the northern parts of Europe with a colder climate, which is a region previously free of this disease. Our study investigates the occurrence of canine babesiosis in different seasons over 2013-2016, and explores a short-term link between meteorological parameters and number of diagnosed cases of canine babesiosis in Belgrade. It also looks into possible long-term correlations that span one year before the onset of the disease. Based on 872 recorded cases over four years, our results show a bimodal seasonal distribution of canine babesiosis, with a pronounced peak in the spring, and a less conspicuous one in the autumn. Throughout the year, even over the coldest and warmest periods, there is a broad range of temperatures and relative humidifies when the disease is recorded. Over one year prior to the spring and autumn onset of the disease, we found a noticeable impact of temperature and relative humidity, and to a lesser extent, of atmospheric pressure and cloud cover, on the number of diagnosed cases. These findings imply short-term and long-term relationships between occurrence of acute canine babesiosis and certain meteorological parameters, and they open further questions that need to be investigated in order to understand the epidemiology of this disease.",
publisher = "Elsevier Gmbh, Munich",
journal = "Ticks and Tick-Borne Diseases",
title = "A short-term and long-term relationship between occurrence of acute canine babesiosis and meteorological parameters in Belgrade, Serbia",
volume = "10",
number = "6",
pages = "UNSP 101273",
doi = "10.1016/j.ttbdis.2019.101273"
}

Janjić, F., Sarvan, D., Tomanović, S., Cuk, J., Krstić, V., Radonjić, V., Kovačević-Filipović, M.,& Ajtić, J.. (2019). A short-term and long-term relationship between occurrence of acute canine babesiosis and meteorological parameters in Belgrade, Serbia. in Ticks and Tick-Borne Diseases
Elsevier Gmbh, Munich., 10(6), UNSP 101273.
https://doi.org/10.1016/j.ttbdis.2019.101273

Janjić F, Sarvan D, Tomanović S, Cuk J, Krstić V, Radonjić V, Kovačević-Filipović M, Ajtić J. A short-term and long-term relationship between occurrence of acute canine babesiosis and meteorological parameters in Belgrade, Serbia. in Ticks and Tick-Borne Diseases. 2019;10(6):UNSP 101273.
doi:10.1016/j.ttbdis.2019.101273 .

Janjić, Filip, Sarvan, Darko, Tomanović, Snežana, Cuk, Jelena, Krstić, Vanja, Radonjić, Vladimir, Kovačević-Filipović, Milica, Ajtić, Jelena, "A short-term and long-term relationship between occurrence of acute canine babesiosis and meteorological parameters in Belgrade, Serbia" in Ticks and Tick-Borne Diseases, 10, no. 6 (2019):UNSP 101273,
https://doi.org/10.1016/j.ttbdis.2019.101273 . .

TY  - JOUR
AU  - Bogunović, Danica
AU  - Stević, Nataša
AU  - Sidi-Boumedine, Karim
AU  - Mišić, Dušan
AU  - Tomanović, Snežana
AU  - Kulišić, Zoran
AU  - Magaš, Vladimir
AU  - Radojičić, Sonja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1589
AB  - Q fever is a zoonotic disease caused by Coxiella burnetii, a gram-negative coccobacillus, which has been detected in a wide range of animal species, mostly domestic ruminants, but also in wild mammals, pets, birds, reptiles, arthropods (especially ticks), as well as in humans. Although the exposure to domestic animals in rural areas is regarded as the most common cause of the disease in humans, recent studies have shown that the role of pets in the epidemiology of Q fever has been increasingly growing. Although the primary route of infection is inhalation, it is presumed that among animals the infection circulates through ticks and that they are responsible for heterospecific transmission, as well as spatial dispersion among vertebrates. The aim of this study was to determine the presence and prevalence of C. burnetii in ticks removed from stray dogs, as well as to examine the distribution of tick species parasitizing dogs on the territory of Belgrade city. A PCR protocol targeting IS1111 repetitive transposon-like region of C. burnetii was used for the detection of C. burnetii DNA in ticks and the results were confirmed by sequence analysis. In total, 316 ticks were collected from 51 stray dogs - 40 females (78.43%) and 11 males (21.57%). Three species of ticks were identified: Rhipicephalus sanguineus (72.15%), Ixodes ricinus (27.53%) and Dermacentor reticulatus (0.32%). Out of 316 examined ticks, C. burnetii DNA was detected only in the brown dog tick R. sanguineus, with a total prevalence of 10.53% (24/228). The high prevalence of C. burnetii in R. sanguineus, which is primarily a dog tick, indicates the importance of dogs in the epidemiology of Q fever in the territory of Belgrade.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia)
VL  - 68
IS  - 3
SP  - 257
EP  - 268
DO  - 10.2478/acve-2018-0023
ER  - 

@article{
author = "Bogunović, Danica and Stević, Nataša and Sidi-Boumedine, Karim and Mišić, Dušan and Tomanović, Snežana and Kulišić, Zoran and Magaš, Vladimir and Radojičić, Sonja",
year = "2018",
abstract = "Q fever is a zoonotic disease caused by Coxiella burnetii, a gram-negative coccobacillus, which has been detected in a wide range of animal species, mostly domestic ruminants, but also in wild mammals, pets, birds, reptiles, arthropods (especially ticks), as well as in humans. Although the exposure to domestic animals in rural areas is regarded as the most common cause of the disease in humans, recent studies have shown that the role of pets in the epidemiology of Q fever has been increasingly growing. Although the primary route of infection is inhalation, it is presumed that among animals the infection circulates through ticks and that they are responsible for heterospecific transmission, as well as spatial dispersion among vertebrates. The aim of this study was to determine the presence and prevalence of C. burnetii in ticks removed from stray dogs, as well as to examine the distribution of tick species parasitizing dogs on the territory of Belgrade city. A PCR protocol targeting IS1111 repetitive transposon-like region of C. burnetii was used for the detection of C. burnetii DNA in ticks and the results were confirmed by sequence analysis. In total, 316 ticks were collected from 51 stray dogs - 40 females (78.43%) and 11 males (21.57%). Three species of ticks were identified: Rhipicephalus sanguineus (72.15%), Ixodes ricinus (27.53%) and Dermacentor reticulatus (0.32%). Out of 316 examined ticks, C. burnetii DNA was detected only in the brown dog tick R. sanguineus, with a total prevalence of 10.53% (24/228). The high prevalence of C. burnetii in R. sanguineus, which is primarily a dog tick, indicates the importance of dogs in the epidemiology of Q fever in the territory of Belgrade.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia)",
volume = "68",
number = "3",
pages = "257-268",
doi = "10.2478/acve-2018-0023"
}

Bogunović, D., Stević, N., Sidi-Boumedine, K., Mišić, D., Tomanović, S., Kulišić, Z., Magaš, V.,& Radojičić, S.. (2018). Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia). in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(3), 257-268.
https://doi.org/10.2478/acve-2018-0023

Bogunović D, Stević N, Sidi-Boumedine K, Mišić D, Tomanović S, Kulišić Z, Magaš V, Radojičić S. Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia). in Acta Veterinaria-Beograd. 2018;68(3):257-268.
doi:10.2478/acve-2018-0023 .

Bogunović, Danica, Stević, Nataša, Sidi-Boumedine, Karim, Mišić, Dušan, Tomanović, Snežana, Kulišić, Zoran, Magaš, Vladimir, Radojičić, Sonja, "Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia)" in Acta Veterinaria-Beograd, 68, no. 3 (2018):257-268,
https://doi.org/10.2478/acve-2018-0023 . .

TY  - JOUR
AU  - Sukara, Ratko
AU  - Chochlakis, Dimosthenis
AU  - Ćirović, Duško
AU  - Penezić, Aleksandra
AU  - Mihaljica, Darko
AU  - Čakić, Sanja
AU  - Valčić, Miroslav
AU  - Tselentis, Yannis
AU  - Psaroulaki, Anna
AU  - Tomanović, Snežana
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1668
AB  - The golden jackal (Canis aureus) is a medium-sized canid species native to Europe. This species is characterized by rapid large-scale expansion. A similar trend is also observed in Serbia, where the species is now distributed in more than a half of the territory. Although jackals prefer habitats in human-dominated landscapes, these animals have not been studied well enough from an eco-epidemiological point of view, and little is known about their potential for carrying zoonotic pathogens. In a study conducted during a three-year period (01/2010-02/2013), a total of 216 hunted or road-killed golden jackals were collected from 10 localities in Serbia. Ticks, when present, were removed, and after necropsy, spleen samples were collected from each animal. All tick and spleen samples were tested for the DNA of bacterial and protozoan tick-borne pathogens (Borrelia species, Bartonella species, Rickettsia species, Anaplasma species, Coxiella burnetii, Francisella species and Babesia species) by multiplex real-time PCR, conventional PCR and sequencing analyses. The DNA of Babesia canis was detected in nine out of 216 (4.2%) spleen samples, and two samples (0.9%) tested positive for Anaplasma phagocytophilum. In 118 ticks collected from jackals, the DNA of two Babesia species (Ba. canis and Ba. microti), three Borrelia species (Bo. garinii, Bo. valaisiana, and Bo. lusitaniae) and A. marginale was detected. From the aspect of public health surveillance, the potential role of the golden jackal in the maintenance of vector-borne zoonotic pathogens in Serbia must be considered, and further eco-epidemiological studies should be performed to determine the precise role of this animal species in zoonotic disease transmission cycles.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Ticks and Tick-Borne Diseases
T1  - Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia
VL  - 9
IS  - 5
SP  - 1090
EP  - 1097
DO  - 10.1016/j.ttbdis.2018.04.003
ER  - 

@article{
author = "Sukara, Ratko and Chochlakis, Dimosthenis and Ćirović, Duško and Penezić, Aleksandra and Mihaljica, Darko and Čakić, Sanja and Valčić, Miroslav and Tselentis, Yannis and Psaroulaki, Anna and Tomanović, Snežana",
year = "2018",
abstract = "The golden jackal (Canis aureus) is a medium-sized canid species native to Europe. This species is characterized by rapid large-scale expansion. A similar trend is also observed in Serbia, where the species is now distributed in more than a half of the territory. Although jackals prefer habitats in human-dominated landscapes, these animals have not been studied well enough from an eco-epidemiological point of view, and little is known about their potential for carrying zoonotic pathogens. In a study conducted during a three-year period (01/2010-02/2013), a total of 216 hunted or road-killed golden jackals were collected from 10 localities in Serbia. Ticks, when present, were removed, and after necropsy, spleen samples were collected from each animal. All tick and spleen samples were tested for the DNA of bacterial and protozoan tick-borne pathogens (Borrelia species, Bartonella species, Rickettsia species, Anaplasma species, Coxiella burnetii, Francisella species and Babesia species) by multiplex real-time PCR, conventional PCR and sequencing analyses. The DNA of Babesia canis was detected in nine out of 216 (4.2%) spleen samples, and two samples (0.9%) tested positive for Anaplasma phagocytophilum. In 118 ticks collected from jackals, the DNA of two Babesia species (Ba. canis and Ba. microti), three Borrelia species (Bo. garinii, Bo. valaisiana, and Bo. lusitaniae) and A. marginale was detected. From the aspect of public health surveillance, the potential role of the golden jackal in the maintenance of vector-borne zoonotic pathogens in Serbia must be considered, and further eco-epidemiological studies should be performed to determine the precise role of this animal species in zoonotic disease transmission cycles.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Ticks and Tick-Borne Diseases",
title = "Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia",
volume = "9",
number = "5",
pages = "1090-1097",
doi = "10.1016/j.ttbdis.2018.04.003"
}

Sukara, R., Chochlakis, D., Ćirović, D., Penezić, A., Mihaljica, D., Čakić, S., Valčić, M., Tselentis, Y., Psaroulaki, A.,& Tomanović, S.. (2018). Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia. in Ticks and Tick-Borne Diseases
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 9(5), 1090-1097.
https://doi.org/10.1016/j.ttbdis.2018.04.003

Sukara R, Chochlakis D, Ćirović D, Penezić A, Mihaljica D, Čakić S, Valčić M, Tselentis Y, Psaroulaki A, Tomanović S. Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia. in Ticks and Tick-Borne Diseases. 2018;9(5):1090-1097.
doi:10.1016/j.ttbdis.2018.04.003 .

Sukara, Ratko, Chochlakis, Dimosthenis, Ćirović, Duško, Penezić, Aleksandra, Mihaljica, Darko, Čakić, Sanja, Valčić, Miroslav, Tselentis, Yannis, Psaroulaki, Anna, Tomanović, Snežana, "Golden jackals (Canis aureus) as hosts for ticks and tick-borne pathogens in Serbia" in Ticks and Tick-Borne Diseases, 9, no. 5 (2018):1090-1097,
https://doi.org/10.1016/j.ttbdis.2018.04.003 . .