TY  - JOUR
AU  - Ninković, Milan
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Bugarski, Dejan
AU  - Stević, Nataša
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3869
AB  - Mycoplasma bovis (M. bovis) is a significant pathogen responsible for highly transmissible
mastitis in cattle globally. It primarily spreads through colostrum, milk, and semen. Cows with
persistent infections act as carriers, intermittently releasing the pathogen, making their milk a pivotal
factor in infection transmission. Given the limited seroprevalence surveys in Serbia, this study aimed
to detect M. bovis presence in bulk tank milk (BTM), determine route shedding, and evaluate infection
risks. BTM samples were collected from 115 dairy farms across Serbia, with M. bovis DNA detected
in 11 out of the 115 samples by real-time PCR. Additionally, M. bovis was detected in 1.30% of nasal
swabs sampled from apparently healthy animals. A univariate analysis of the risk factors associated
with M. bovis presence in the BTM samples revealed correlations with factors such as the breed, farm
seropositivity, pre-milking and post-milking disinfection practices, farm type, cow population, milk
yield, number of cows in the BTM samples, and parity. Seropositive farms exhibited the highest
likelihood of M. bovis presence in milk. Moreover, pre- and post-milking disinfection practices and
highly productive cows yielding over 8000 L of milk were identified as risk factors for PCR-positive
BTM. In a multivariable mixed regression analysis, a risk factor for the presence of M. bovis infection
in the BTM sample was the Holstein breed. These findings underscore a relatively high prevalence
of M. bovis in BTM within Serbian dairy farms, suggesting a potential risk for M. bovis spreading
through milk and oral route of calves’ infection.
PB  - MDPI
T2  - Pathogens
T1  - Presence of Mycoplasma bovis in bulk tank milk and associated risk factor analysis in Serbian dairy farms
VL  - 13
IS  - 4
SP  - 302
DO  - 10.3390/pathogens13040302
ER  - 

@article{
author = "Ninković, Milan and Milićević, Vesna and Radojičić, Sonja and Bugarski, Dejan and Stević, Nataša",
year = "2024",
abstract = "Mycoplasma bovis (M. bovis) is a significant pathogen responsible for highly transmissible
mastitis in cattle globally. It primarily spreads through colostrum, milk, and semen. Cows with
persistent infections act as carriers, intermittently releasing the pathogen, making their milk a pivotal
factor in infection transmission. Given the limited seroprevalence surveys in Serbia, this study aimed
to detect M. bovis presence in bulk tank milk (BTM), determine route shedding, and evaluate infection
risks. BTM samples were collected from 115 dairy farms across Serbia, with M. bovis DNA detected
in 11 out of the 115 samples by real-time PCR. Additionally, M. bovis was detected in 1.30% of nasal
swabs sampled from apparently healthy animals. A univariate analysis of the risk factors associated
with M. bovis presence in the BTM samples revealed correlations with factors such as the breed, farm
seropositivity, pre-milking and post-milking disinfection practices, farm type, cow population, milk
yield, number of cows in the BTM samples, and parity. Seropositive farms exhibited the highest
likelihood of M. bovis presence in milk. Moreover, pre- and post-milking disinfection practices and
highly productive cows yielding over 8000 L of milk were identified as risk factors for PCR-positive
BTM. In a multivariable mixed regression analysis, a risk factor for the presence of M. bovis infection
in the BTM sample was the Holstein breed. These findings underscore a relatively high prevalence
of M. bovis in BTM within Serbian dairy farms, suggesting a potential risk for M. bovis spreading
through milk and oral route of calves’ infection.",
publisher = "MDPI",
journal = "Pathogens",
title = "Presence of Mycoplasma bovis in bulk tank milk and associated risk factor analysis in Serbian dairy farms",
volume = "13",
number = "4",
pages = "302",
doi = "10.3390/pathogens13040302"
}

Ninković, M., Milićević, V., Radojičić, S., Bugarski, D.,& Stević, N.. (2024). Presence of Mycoplasma bovis in bulk tank milk and associated risk factor analysis in Serbian dairy farms. in Pathogens
MDPI., 13(4), 302.
https://doi.org/10.3390/pathogens13040302

Ninković M, Milićević V, Radojičić S, Bugarski D, Stević N. Presence of Mycoplasma bovis in bulk tank milk and associated risk factor analysis in Serbian dairy farms. in Pathogens. 2024;13(4):302.
doi:10.3390/pathogens13040302 .

Ninković, Milan, Milićević, Vesna, Radojičić, Sonja, Bugarski, Dejan, Stević, Nataša, "Presence of Mycoplasma bovis in bulk tank milk and associated risk factor analysis in Serbian dairy farms" in Pathogens, 13, no. 4 (2024):302,
https://doi.org/10.3390/pathogens13040302 . .

TY  - CONF
AU  - Radojičić, Sonja
AU  - Stević, Nataša
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3719
AB  - Svake godine svedočimo sve očiglednijim promenama klime, koje
pokazuju svoj poseban uticaj na epizootiološki trougao i njegove
determinante: prijemčivu vrstu, patogene mikroorganizme i okruženje,
odnosno sredinu u kojoj se ova interakcija ostvaruje. U fokusu svetske
naučne javnosti su istraživanja promena klimatskih obrazaca čiji se
uticaj sve češće dovodi u vezu sa interakcijama koje postoje između
patogena i domaćina. Klimatske promene koje nastaju kao posledica
jednog od najmarkantnijih fenomena poznatog kao El NINO (El Niño –
Southern Oscillation (ENSO), imaju kratkoročne, ali izrazite efekte na
vremenske prilike čije su posledice odstupanja od uobičajenih klimatskih
obrazaca. One podrazumevaju poplave, suše, promene spoljašnje
temperature u smeru sve toplijih leta i kao posledicu sve masovnije
i češće šumske požare, ali i izbijanja epidemija i epizootija. Ciklična
pojava ovakvih anomalija u prošlosti, dovodi se u vezu sa epizootijama
zaraznih bolesti poput kuge kopitara, groznice zapadnog Nila, groznice
doline Rift, hantavirusnih infekcija, malarije, kuge, kolere i nastajanjem
povoljnih ekoloških uslova za širenje areala vektora prenosioca
i rezervoara zaraznih bolesti. Na koji način podizanje temperature
vode u Tihom i Indijskom okeanu (ENSO), uz druge efekte čiji kombinovаni
i često nejasni uticaji na promene klime, ugrožavaju ne samo
biodiverzitet, dovode do epizootija, epidemija, i širenja areala prirodno
žarišnih infekcija, izazovi su sa kojima se susreće savremena nauka.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
T1  - Uticaj klimatskih promena na epizootiološke determinante, pojavu i širenje zaraznih bolesti
SP  - 99
EP  - 106
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3719
ER  - 

@conference{
author = "Radojičić, Sonja and Stević, Nataša",
year = "2024",
abstract = "Svake godine svedočimo sve očiglednijim promenama klime, koje
pokazuju svoj poseban uticaj na epizootiološki trougao i njegove
determinante: prijemčivu vrstu, patogene mikroorganizme i okruženje,
odnosno sredinu u kojoj se ova interakcija ostvaruje. U fokusu svetske
naučne javnosti su istraživanja promena klimatskih obrazaca čiji se
uticaj sve češće dovodi u vezu sa interakcijama koje postoje između
patogena i domaćina. Klimatske promene koje nastaju kao posledica
jednog od najmarkantnijih fenomena poznatog kao El NINO (El Niño –
Southern Oscillation (ENSO), imaju kratkoročne, ali izrazite efekte na
vremenske prilike čije su posledice odstupanja od uobičajenih klimatskih
obrazaca. One podrazumevaju poplave, suše, promene spoljašnje
temperature u smeru sve toplijih leta i kao posledicu sve masovnije
i češće šumske požare, ali i izbijanja epidemija i epizootija. Ciklična
pojava ovakvih anomalija u prošlosti, dovodi se u vezu sa epizootijama
zaraznih bolesti poput kuge kopitara, groznice zapadnog Nila, groznice
doline Rift, hantavirusnih infekcija, malarije, kuge, kolere i nastajanjem
povoljnih ekoloških uslova za širenje areala vektora prenosioca
i rezervoara zaraznih bolesti. Na koji način podizanje temperature
vode u Tihom i Indijskom okeanu (ENSO), uz druge efekte čiji kombinovаni
i često nejasni uticaji na promene klime, ugrožavaju ne samo
biodiverzitet, dovode do epizootija, epidemija, i širenja areala prirodno
žarišnih infekcija, izazovi su sa kojima se susreće savremena nauka.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024",
title = "Uticaj klimatskih promena na epizootiološke determinante, pojavu i širenje zaraznih bolesti",
pages = "99-106",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3719"
}

Radojičić, S.,& Stević, N.. (2024). Uticaj klimatskih promena na epizootiološke determinante, pojavu i širenje zaraznih bolesti. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 99-106.
https://hdl.handle.net/21.15107/rcub_veterinar_3719

Radojičić S, Stević N. Uticaj klimatskih promena na epizootiološke determinante, pojavu i širenje zaraznih bolesti. in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024. 2024;:99-106.
https://hdl.handle.net/21.15107/rcub_veterinar_3719 .

Radojičić, Sonja, Stević, Nataša, "Uticaj klimatskih promena na epizootiološke determinante, pojavu i širenje zaraznih bolesti" in XLV seminar za inovacije znanja veterinara, Beograd, 23. februar 2024 (2024):99-106,
https://hdl.handle.net/21.15107/rcub_veterinar_3719 .

TY  - JOUR
AU  - Zelenović, Mladen
AU  - Marinković, Darko
AU  - Stević, Nataša
AU  - Stanojević, Slavoljub
AU  - Aničić, Milan
AU  - Milićević, Vesna
AU  - Valčić, Olivera
AU  - Radojičić, Sonja
PY  - 2024
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3790
AB  - Brucella ovis infects sheep and causes a clinical or subclinical disease characterized by
genital lesions and reduced fertility in rams, placentitis and abortions in ewes, and
increased perinatal mortality in lambs. As part of this study, analyses were conducted
on a sheep farm near Belgrade (Serbia). Of the serological tests, indirect ELISA was
performed. A total of 94 blood sera were analyzed, 33 from rams and 61 from ewes.
The results showed 23 (69.7%) positive findings in rams and 2 (3.3%) positive findings
in ewes, with an overall prevalence of 25.4% bounded by a 95% confidence interval.
Bruce-ladder multiplex PCR and Real time PCR were performed on 19 serologically
positive rams and two serologically positive ewes. The results indicate a slightly
higher sensitivity of Real time PCR compared to conventional PCR in diagnosing
B. ovis from the reproductive tissues of rams. However, the differences in sensitivity
between different nucleic acid extraction protocols were not significant. Most rams
showed a positive PCR result in only one sample of reproductive tissue, suggesting the
recommendation to take multiple samples from each animal. Further research is needed
to bring the sensitivity of molecular tests in diagnosing ram epididymitis closer to the
sensitivity of serological tests.
PB  - Belgrade : Faculty of Veterinary Medicine
T2  - Acta Veterinaria
T1  - Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis
VL  - 74
IS  - 1
SP  - 133
EP  - 144
DO  - 10.2478/acve-2024-0010
ER  - 

@article{
author = "Zelenović, Mladen and Marinković, Darko and Stević, Nataša and Stanojević, Slavoljub and Aničić, Milan and Milićević, Vesna and Valčić, Olivera and Radojičić, Sonja",
year = "2024",
abstract = "Brucella ovis infects sheep and causes a clinical or subclinical disease characterized by
genital lesions and reduced fertility in rams, placentitis and abortions in ewes, and
increased perinatal mortality in lambs. As part of this study, analyses were conducted
on a sheep farm near Belgrade (Serbia). Of the serological tests, indirect ELISA was
performed. A total of 94 blood sera were analyzed, 33 from rams and 61 from ewes.
The results showed 23 (69.7%) positive findings in rams and 2 (3.3%) positive findings
in ewes, with an overall prevalence of 25.4% bounded by a 95% confidence interval.
Bruce-ladder multiplex PCR and Real time PCR were performed on 19 serologically
positive rams and two serologically positive ewes. The results indicate a slightly
higher sensitivity of Real time PCR compared to conventional PCR in diagnosing
B. ovis from the reproductive tissues of rams. However, the differences in sensitivity
between different nucleic acid extraction protocols were not significant. Most rams
showed a positive PCR result in only one sample of reproductive tissue, suggesting the
recommendation to take multiple samples from each animal. Further research is needed
to bring the sensitivity of molecular tests in diagnosing ram epididymitis closer to the
sensitivity of serological tests.",
publisher = "Belgrade : Faculty of Veterinary Medicine",
journal = "Acta Veterinaria",
title = "Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis",
volume = "74",
number = "1",
pages = "133-144",
doi = "10.2478/acve-2024-0010"
}

Zelenović, M., Marinković, D., Stević, N., Stanojević, S., Aničić, M., Milićević, V., Valčić, O.,& Radojičić, S.. (2024). Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis. in Acta Veterinaria
Belgrade : Faculty of Veterinary Medicine., 74(1), 133-144.
https://doi.org/10.2478/acve-2024-0010

Zelenović M, Marinković D, Stević N, Stanojević S, Aničić M, Milićević V, Valčić O, Radojičić S. Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis. in Acta Veterinaria. 2024;74(1):133-144.
doi:10.2478/acve-2024-0010 .

Zelenović, Mladen, Marinković, Darko, Stević, Nataša, Stanojević, Slavoljub, Aničić, Milan, Milićević, Vesna, Valčić, Olivera, Radojičić, Sonja, "Reliability of molecular tests in diagnosing ovine brucellosis caused by Brucella ovis" in Acta Veterinaria, 74, no. 1 (2024):133-144,
https://doi.org/10.2478/acve-2024-0010 . .

TY  - JOUR
AU  - Glišić, Dimitrije
AU  - Milićević, Vesna
AU  - Krnjaić, Dejan
AU  - Toplak, Ivan
AU  - Prodanović, Radiša
AU  - Gallardo, Carmina
AU  - Radojičić, Sonja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2947
AB  - This study provides the frst comprehensive report on the molecular characteristics of African swine fever virus (ASFV) variants
in Serbia between 2019 and 2022. Since its frst observation in July 2019, the disease has been found in wild boar and domestic
swine. The study involved the analysis of 95 ASFV-positive samples collected from 12 infected administrative districts in Serbia.
Partial four genomic regions were genetically characterized, including B646L, E183L, B602L, and the intergenic region (IGR)
between the I73R-I329L genes. The results of the study suggest that multiple ASFV strains belonging to genotype II are circulating
in Serbia, as evidenced by the analysis of the IGR between I73R-I329L genes that showed the most diferences. Furthermore,
the phylogenetic analysis of the B602L gene showed three diferent clades within the CVR I group of ASFV strains. Regarding
the IGR, 98.4% were grouped into IGR II, with only one positive sample grouped into the IGR III group. These fndings provide
essential insights into the molecular characteristics of ASFV variants in Serbia and contribute to the knowledge of circulating
strains of ASFV in Europe. However, further research is necessary to gain a better understanding of ASFV spread and evolution
PB  - Springer
T2  - Veterinary Research Communications
T1  - Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia
VL  - 47
IS  - 4
SP  - 1925
EP  - 1936
DO  - 10.1007/s11259-023-10145-7
ER  - 

@article{
author = "Glišić, Dimitrije and Milićević, Vesna and Krnjaić, Dejan and Toplak, Ivan and Prodanović, Radiša and Gallardo, Carmina and Radojičić, Sonja",
year = "2023",
abstract = "This study provides the frst comprehensive report on the molecular characteristics of African swine fever virus (ASFV) variants
in Serbia between 2019 and 2022. Since its frst observation in July 2019, the disease has been found in wild boar and domestic
swine. The study involved the analysis of 95 ASFV-positive samples collected from 12 infected administrative districts in Serbia.
Partial four genomic regions were genetically characterized, including B646L, E183L, B602L, and the intergenic region (IGR)
between the I73R-I329L genes. The results of the study suggest that multiple ASFV strains belonging to genotype II are circulating
in Serbia, as evidenced by the analysis of the IGR between I73R-I329L genes that showed the most diferences. Furthermore,
the phylogenetic analysis of the B602L gene showed three diferent clades within the CVR I group of ASFV strains. Regarding
the IGR, 98.4% were grouped into IGR II, with only one positive sample grouped into the IGR III group. These fndings provide
essential insights into the molecular characteristics of ASFV variants in Serbia and contribute to the knowledge of circulating
strains of ASFV in Europe. However, further research is necessary to gain a better understanding of ASFV spread and evolution",
publisher = "Springer",
journal = "Veterinary Research Communications",
title = "Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia",
volume = "47",
number = "4",
pages = "1925-1936",
doi = "10.1007/s11259-023-10145-7"
}

Glišić, D., Milićević, V., Krnjaić, D., Toplak, I., Prodanović, R., Gallardo, C.,& Radojičić, S.. (2023). Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia. in Veterinary Research Communications
Springer., 47(4), 1925-1936.
https://doi.org/10.1007/s11259-023-10145-7

Glišić D, Milićević V, Krnjaić D, Toplak I, Prodanović R, Gallardo C, Radojičić S. Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia. in Veterinary Research Communications. 2023;47(4):1925-1936.
doi:10.1007/s11259-023-10145-7 .

Glišić, Dimitrije, Milićević, Vesna, Krnjaić, Dejan, Toplak, Ivan, Prodanović, Radiša, Gallardo, Carmina, Radojičić, Sonja, "Genetic analysis reveals multiple intergenic region and central variable region in the African swine fever virus variants circulating in Serbia" in Veterinary Research Communications, 47, no. 4 (2023):1925-1936,
https://doi.org/10.1007/s11259-023-10145-7 . .

TY  - CONF
AU  - Glišić, Dimitrije
AU  - Milićević, Vesna
AU  - Krnjaić, Dejan
AU  - Prodanović, Radiša
AU  - Toplak, Ivan
AU  - Radojičić, Sonja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3208
AB  - Afrička kuga svinja (AKS) je virusno hemoragično oboljenje domaćih i divljih
svinja visokog stepena letaliteta, koje uzrokuje enormne ekonomske gubitke u
industriji svinja. Uzročnik afričke kuge je istoimeni virus, jedini pripadnik familije
Asfarviride i roda Asfivirus (Alonso i sar., 2018). Bolest je prvi put zabeležena u Srbiji
2019. godine u populaciji domaćih svinja, a od 2020. godine i u populaciji divljih svinja.
Od tada do danas broj svinja je smanjen za 7% što je više od prosečnog pada broja
svinja u Evropi od 4%. Genom virusa AKS je dvolančana linearna DNK dužine 170-190
kbp u zavisnosti od genotipa, podgenotipa i broja ponavljajućih segmenata i kodira
sintezu 170 virusnih proteina (Blome i sar., 2020). Održavanje virusa u prirodi zavisi
od faktora sredine, prisustva prijemčivih jedinki (divljih svinja) i otpornosti virusa.
Jedan od ključnih uslova za održavanje i cirkulaciju virusa je i visoka prevalencija
infekcije kod domaćih svinja (>5%) jer se na taj način omogućava prelivanje infekcije
na divlje svinje i zatvaranje kruga prenošenja (Aguilar-Vega i sar., 2023). Letalitet kod
divljih svinja inficiranih visoko virulentnim sojem iznosi 100%. Virus ostaje infektivan
3 meseca u leševima divljih svinja koje, ukoliko nisu uklonjene, dugotrajno
predstavljaju izvor infekcije. Brzina širenja infekcije u populaciji divljih svinja u Evropi
iznosi 4-17 km godišnje (Anette i sar., 2020). Širenje AKS na veće razdaljine uslovljeno
je antropogenim faktorom. Čovek kontaktom sa zaraženim ili uginulim divljim
svinjama, hranjenjem svinja kontaminiranim proizvodima animalnog porekla,
transportom zaraženih domaćih svinja iz jednog područija u drugo predstavlja
mehanički vektor za širenje virusa. Na afričkom kontinentu zabeležena su 24 genotipa
virusa koja cirkulišu između populacija bradavičastih svinja, mekih krpelja iz roda
Ornithodorus spp. i domaćih svinja. Van Afrike zabeleženi su samo genotip I i genotip
II virusa AKS. Za trenutnu epizootiju odgovoran je genotip II virusa. Autori Gallardo i
sar. (2023) su na osnovu analize genoma virusa AKS zabaležili 24 genogrupe u okviru genotipa II virusa na tlu Evrope. Na osnovu analize B646L i E183L gena opisan je jedan
genotip virusa (genotip II). Daljom analizom B602L gena definisana su dva
podgenotipa (CVR1 i 2) sa većim brojem varijacija koje zavise od broja tačkastih
mutacija. Na osnovu analize intergenskog segmenta (I73L - I329L), mogu se opisati
četiri podgenotipa (I-IV) (Gallardo i sar., 2023).
Laboratorijska dijagnostika afričke kuge se vrši primenom molekularnih metoda.
Cilj ovog rada je bila analiza cikulišućih sojeva virusa AKS u Srbiji. Trijažna testiranja
su vršena real-time PCR testom. Dok je dalja analiza vršena primenom klasičnog PCR
testa i Sanger sekvenciranja. Za detaljnu karakterizaciju lokalnih sojeva virusa AKS
odabrano je 95 uzoraka poreklom od domacih i divljih svinja obolelih od AKS u
periodu od 2019-2023 godine. Amplifikovana su i sekvencirana četiri segmenta
virusnog genoma (B646L, E183L, B602L i intergenski region između I73L - I329L). Na
osnovu analize ovih delova genoma utvrđeno je prisustvo više različitih sojeva virusa
u Srbiji. Istovremena cirkulacija nekoliko sojeva može biti posledica spontanih
mutacija ili reintrodukcije virusa iz drugih zaraženih područja. Utvrđivanje promena
u genskom materijalu smatraju se veoma značajnim informacijama jer mogu ukazivati
na adaptaciju virusa i modifikaciju virulentnosti. Praćenje promena u genomu virusa,
porsedno daje uvid u strukturu virusnih proteina. Dugotrajna cirkulacija virusa u
populaciji divljih svinja može dovesti do pojave srednje i niskovirulentnih sojeva što
predstavlja izazov za eradikaciju bolesti. Kod domaćih svinja, srednje i niskovirulentni
sojevi mogu izazvati atipične kliničke znakove i produženi tok bolesti što otežava
postavljanje sumnje, odlaže dijagnostiku i povećava rizik od širenja bolesti.
Potencijalne promene u konzerviranom delu genoma mogu otežati dijagnostiku
virusa. Stoga su izolacija i genska karakterizacija virusa afričke kuge svinja od
izuzetnog značaja za razumevanje epizootiologije ove bolesti. Poznavanje genskih
karakteristika lokalnih sojeva virusa omogućava praćenje njihovog evolutivnog
razvoja i identifikaciju novih sojeva. Na osnovu ovih podataka treba vršiti procenu
efikasnosti preduzetih mera za suzbijanje i kontrolu bolesti. Usled nedostatka
komercijalno dostupne vackcine, primena rigoroznih biosigurnostih mera je od
najvećeg značaja za sprečavanje unosa uzročnika u populaciju domaćih svinja. Stroge
kontrole na granicama su neophodne radi sprečavanja reintrodukcije virusa iz drugih
zaraženih područija, kao i brza reakcija u aktivnim žarištima radi sprečavanja daljeg
širenja bolesti i većih ekonomskih gubitaka.
AB  - African Swine Fever (ASF) is a highly lethal viral hemorrhagic disease affecting
domestic and wild pigs, causing significant economic losses in the swine industry. The
causative agent of ASF is a virus of the Asfarviridae family and Asfivirus genus (Alonso
et al., 2018). The disease was first recorded in Serbia in 2019 in domestic pig
populations, and since 2020, it has also been found in wild pig populations. Since then,
the number of pigs has decreased by 7%, which is higher than the average decline in
pig numbers in Europe (4%). The genome of the ASF virus is a double-stranded linear
DNA, ranging from 170-190 kbp in length, depending on the genotype, subgenotype,
and number of repetitive segments, encoding the synthesis of 170 viral proteins
(Blome et al., 2020). The maintenance of the virus in the environment depends on
environmental factors, the presence of susceptible individuals (wild pigs), and the
virus's ability to survive in that environment. A key condition for the maintenance and
circulation of the virus is a high prevalence of infection in domestic pigs (>5%), as it
enables the spill-over of infection to wild pigs, completing the transmission cycle
(Aguilar-Vega et al., 2023). The case fatality rate in wild pigs infected with highly
virulent strains is 100%. The virus remains infectious for 3 months in carcasses of wild
pigs, which, if not removed, serve as a long-term source of infection. The spread of
infection in the wild pig population in Europe is estimated at 4-17 km per year, with
anthropogenic factors playing a significant role in long-distance transmission (Anette
et al., 2020). Human contact with infected or dead wild pigs, feeding pigs with
contaminated animal-derived products, and the transport of infected domestic pigs
from one area to another act as mechanical vectors for virus spread. In Africa, 24
genotypes of the virus have been recorded, circulating among populations of bush
pigs, soft ticks of the Ornithodorus spp. genus, and domestic pigs. Outside of Africa,
only genotype I and genotype II of the ASF virus have been identified. The current epidemic is caused by the genotype II virus. Based on the genome analysis of the ASF
virus Gallardo et al. (2023), identified 24 genogroups within the genotype II virus in
Europe. From the analysis of the B646L and E183L genes, one virus strain (genotype
II) was described. Further analysis of the B602L gene defined two subgenotypes
(CVR1 and 2) with a higher number of variations depending on the number of point
mutations. Based on the analysis of the intergenic segment (I73L - I329L), four
subgenotypes (I-IV) can be described (Gallardo et al., 2023). Laboratory diagnostics of
African Swine Fever are conducted using molecular methods. The aim of this study
was to analyze circulating strains of ASF virus in Serbia. Screening tests were
performed using real-time PCR, while further analysis was conducted using
conventional PCR and Sanger sequencing. For a detailed characterization of local
strains of ASF virus, 95 samples from diseased domestic and wild pigs with ASF
between 2019 and 2023 were selected. Four segments of the viral genome (B646L,
E183L, B602L, and the intergenic region between I73L - I329L) were amplified and
sequenced. Based on the analysis of these genome segments, the presence of multiple
different strains of the virus was identified in Serbia. Simultaneous circulation of
several strains may result from spontaneous mutations or virus reintroduction from
other infected areas. Determining changes in the genetic material is considered highly
significant as they can indicate virus adaptation and modification of virulence.
Monitoring changes in the virus genome provides insight into the structure of viral
proteins. Prolonged circulation of the virus in the wild pig population can lead to the
emergence of moderately and low-virulent strains, posing challenges for disease
eradication. In domestic pigs, these strains can cause atypical clinical signs and
prolonged disease courses, complicating suspicion, delaying diagnosis, and increasing
the risk of disease spread. Potential changes in conserved regions of the genome can
hinder virus diagnostics. Therefore, the isolation and genetic characterization of ASF
virus strains are of utmost importance for understanding the epizootiology of this
disease. Knowledge of the genetic characteristics of local virus strains allows for
monitoring their evolutionary development and identification of new strains. Based
on these data, an assessment of the effectiveness of control measures should be
conducted. Due to the lack of commercially available vaccines, the implementation of
rigorous biosecurity measures is of paramount importance to prevent the
introduction of the pathogen into the domestic pig population. Strict controls at
borders are necessary to prevent the reintroduction of the virus from other infected
areas, along with swift responses in active outbreak areas to prevent further disease
spread and mitigate significant economic losses.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Genska varijabilnost virusa afričke kuge svinja u Srbiji
T1  - Genetic variability of African Swine Fever virus in Serbia
SP  - 24
EP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3208
ER  - 

@conference{
author = "Glišić, Dimitrije and Milićević, Vesna and Krnjaić, Dejan and Prodanović, Radiša and Toplak, Ivan and Radojičić, Sonja",
year = "2023",
abstract = "Afrička kuga svinja (AKS) je virusno hemoragično oboljenje domaćih i divljih
svinja visokog stepena letaliteta, koje uzrokuje enormne ekonomske gubitke u
industriji svinja. Uzročnik afričke kuge je istoimeni virus, jedini pripadnik familije
Asfarviride i roda Asfivirus (Alonso i sar., 2018). Bolest je prvi put zabeležena u Srbiji
2019. godine u populaciji domaćih svinja, a od 2020. godine i u populaciji divljih svinja.
Od tada do danas broj svinja je smanjen za 7% što je više od prosečnog pada broja
svinja u Evropi od 4%. Genom virusa AKS je dvolančana linearna DNK dužine 170-190
kbp u zavisnosti od genotipa, podgenotipa i broja ponavljajućih segmenata i kodira
sintezu 170 virusnih proteina (Blome i sar., 2020). Održavanje virusa u prirodi zavisi
od faktora sredine, prisustva prijemčivih jedinki (divljih svinja) i otpornosti virusa.
Jedan od ključnih uslova za održavanje i cirkulaciju virusa je i visoka prevalencija
infekcije kod domaćih svinja (>5%) jer se na taj način omogućava prelivanje infekcije
na divlje svinje i zatvaranje kruga prenošenja (Aguilar-Vega i sar., 2023). Letalitet kod
divljih svinja inficiranih visoko virulentnim sojem iznosi 100%. Virus ostaje infektivan
3 meseca u leševima divljih svinja koje, ukoliko nisu uklonjene, dugotrajno
predstavljaju izvor infekcije. Brzina širenja infekcije u populaciji divljih svinja u Evropi
iznosi 4-17 km godišnje (Anette i sar., 2020). Širenje AKS na veće razdaljine uslovljeno
je antropogenim faktorom. Čovek kontaktom sa zaraženim ili uginulim divljim
svinjama, hranjenjem svinja kontaminiranim proizvodima animalnog porekla,
transportom zaraženih domaćih svinja iz jednog područija u drugo predstavlja
mehanički vektor za širenje virusa. Na afričkom kontinentu zabeležena su 24 genotipa
virusa koja cirkulišu između populacija bradavičastih svinja, mekih krpelja iz roda
Ornithodorus spp. i domaćih svinja. Van Afrike zabeleženi su samo genotip I i genotip
II virusa AKS. Za trenutnu epizootiju odgovoran je genotip II virusa. Autori Gallardo i
sar. (2023) su na osnovu analize genoma virusa AKS zabaležili 24 genogrupe u okviru genotipa II virusa na tlu Evrope. Na osnovu analize B646L i E183L gena opisan je jedan
genotip virusa (genotip II). Daljom analizom B602L gena definisana su dva
podgenotipa (CVR1 i 2) sa većim brojem varijacija koje zavise od broja tačkastih
mutacija. Na osnovu analize intergenskog segmenta (I73L - I329L), mogu se opisati
četiri podgenotipa (I-IV) (Gallardo i sar., 2023).
Laboratorijska dijagnostika afričke kuge se vrši primenom molekularnih metoda.
Cilj ovog rada je bila analiza cikulišućih sojeva virusa AKS u Srbiji. Trijažna testiranja
su vršena real-time PCR testom. Dok je dalja analiza vršena primenom klasičnog PCR
testa i Sanger sekvenciranja. Za detaljnu karakterizaciju lokalnih sojeva virusa AKS
odabrano je 95 uzoraka poreklom od domacih i divljih svinja obolelih od AKS u
periodu od 2019-2023 godine. Amplifikovana su i sekvencirana četiri segmenta
virusnog genoma (B646L, E183L, B602L i intergenski region između I73L - I329L). Na
osnovu analize ovih delova genoma utvrđeno je prisustvo više različitih sojeva virusa
u Srbiji. Istovremena cirkulacija nekoliko sojeva može biti posledica spontanih
mutacija ili reintrodukcije virusa iz drugih zaraženih područja. Utvrđivanje promena
u genskom materijalu smatraju se veoma značajnim informacijama jer mogu ukazivati
na adaptaciju virusa i modifikaciju virulentnosti. Praćenje promena u genomu virusa,
porsedno daje uvid u strukturu virusnih proteina. Dugotrajna cirkulacija virusa u
populaciji divljih svinja može dovesti do pojave srednje i niskovirulentnih sojeva što
predstavlja izazov za eradikaciju bolesti. Kod domaćih svinja, srednje i niskovirulentni
sojevi mogu izazvati atipične kliničke znakove i produženi tok bolesti što otežava
postavljanje sumnje, odlaže dijagnostiku i povećava rizik od širenja bolesti.
Potencijalne promene u konzerviranom delu genoma mogu otežati dijagnostiku
virusa. Stoga su izolacija i genska karakterizacija virusa afričke kuge svinja od
izuzetnog značaja za razumevanje epizootiologije ove bolesti. Poznavanje genskih
karakteristika lokalnih sojeva virusa omogućava praćenje njihovog evolutivnog
razvoja i identifikaciju novih sojeva. Na osnovu ovih podataka treba vršiti procenu
efikasnosti preduzetih mera za suzbijanje i kontrolu bolesti. Usled nedostatka
komercijalno dostupne vackcine, primena rigoroznih biosigurnostih mera je od
najvećeg značaja za sprečavanje unosa uzročnika u populaciju domaćih svinja. Stroge
kontrole na granicama su neophodne radi sprečavanja reintrodukcije virusa iz drugih
zaraženih područija, kao i brza reakcija u aktivnim žarištima radi sprečavanja daljeg
širenja bolesti i većih ekonomskih gubitaka., African Swine Fever (ASF) is a highly lethal viral hemorrhagic disease affecting
domestic and wild pigs, causing significant economic losses in the swine industry. The
causative agent of ASF is a virus of the Asfarviridae family and Asfivirus genus (Alonso
et al., 2018). The disease was first recorded in Serbia in 2019 in domestic pig
populations, and since 2020, it has also been found in wild pig populations. Since then,
the number of pigs has decreased by 7%, which is higher than the average decline in
pig numbers in Europe (4%). The genome of the ASF virus is a double-stranded linear
DNA, ranging from 170-190 kbp in length, depending on the genotype, subgenotype,
and number of repetitive segments, encoding the synthesis of 170 viral proteins
(Blome et al., 2020). The maintenance of the virus in the environment depends on
environmental factors, the presence of susceptible individuals (wild pigs), and the
virus's ability to survive in that environment. A key condition for the maintenance and
circulation of the virus is a high prevalence of infection in domestic pigs (>5%), as it
enables the spill-over of infection to wild pigs, completing the transmission cycle
(Aguilar-Vega et al., 2023). The case fatality rate in wild pigs infected with highly
virulent strains is 100%. The virus remains infectious for 3 months in carcasses of wild
pigs, which, if not removed, serve as a long-term source of infection. The spread of
infection in the wild pig population in Europe is estimated at 4-17 km per year, with
anthropogenic factors playing a significant role in long-distance transmission (Anette
et al., 2020). Human contact with infected or dead wild pigs, feeding pigs with
contaminated animal-derived products, and the transport of infected domestic pigs
from one area to another act as mechanical vectors for virus spread. In Africa, 24
genotypes of the virus have been recorded, circulating among populations of bush
pigs, soft ticks of the Ornithodorus spp. genus, and domestic pigs. Outside of Africa,
only genotype I and genotype II of the ASF virus have been identified. The current epidemic is caused by the genotype II virus. Based on the genome analysis of the ASF
virus Gallardo et al. (2023), identified 24 genogroups within the genotype II virus in
Europe. From the analysis of the B646L and E183L genes, one virus strain (genotype
II) was described. Further analysis of the B602L gene defined two subgenotypes
(CVR1 and 2) with a higher number of variations depending on the number of point
mutations. Based on the analysis of the intergenic segment (I73L - I329L), four
subgenotypes (I-IV) can be described (Gallardo et al., 2023). Laboratory diagnostics of
African Swine Fever are conducted using molecular methods. The aim of this study
was to analyze circulating strains of ASF virus in Serbia. Screening tests were
performed using real-time PCR, while further analysis was conducted using
conventional PCR and Sanger sequencing. For a detailed characterization of local
strains of ASF virus, 95 samples from diseased domestic and wild pigs with ASF
between 2019 and 2023 were selected. Four segments of the viral genome (B646L,
E183L, B602L, and the intergenic region between I73L - I329L) were amplified and
sequenced. Based on the analysis of these genome segments, the presence of multiple
different strains of the virus was identified in Serbia. Simultaneous circulation of
several strains may result from spontaneous mutations or virus reintroduction from
other infected areas. Determining changes in the genetic material is considered highly
significant as they can indicate virus adaptation and modification of virulence.
Monitoring changes in the virus genome provides insight into the structure of viral
proteins. Prolonged circulation of the virus in the wild pig population can lead to the
emergence of moderately and low-virulent strains, posing challenges for disease
eradication. In domestic pigs, these strains can cause atypical clinical signs and
prolonged disease courses, complicating suspicion, delaying diagnosis, and increasing
the risk of disease spread. Potential changes in conserved regions of the genome can
hinder virus diagnostics. Therefore, the isolation and genetic characterization of ASF
virus strains are of utmost importance for understanding the epizootiology of this
disease. Knowledge of the genetic characteristics of local virus strains allows for
monitoring their evolutionary development and identification of new strains. Based
on these data, an assessment of the effectiveness of control measures should be
conducted. Due to the lack of commercially available vaccines, the implementation of
rigorous biosecurity measures is of paramount importance to prevent the
introduction of the pathogen into the domestic pig population. Strict controls at
borders are necessary to prevent the reintroduction of the virus from other infected
areas, along with swift responses in active outbreak areas to prevent further disease
spread and mitigate significant economic losses.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Genska varijabilnost virusa afričke kuge svinja u Srbiji, Genetic variability of African Swine Fever virus in Serbia",
pages = "24-28",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3208"
}

Glišić, D., Milićević, V., Krnjaić, D., Prodanović, R., Toplak, I.,& Radojičić, S.. (2023). Genska varijabilnost virusa afričke kuge svinja u Srbiji. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 24-28.
https://hdl.handle.net/21.15107/rcub_veterinar_3208

Glišić D, Milićević V, Krnjaić D, Prodanović R, Toplak I, Radojičić S. Genska varijabilnost virusa afričke kuge svinja u Srbiji. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:24-28.
https://hdl.handle.net/21.15107/rcub_veterinar_3208 .

Glišić, Dimitrije, Milićević, Vesna, Krnjaić, Dejan, Prodanović, Radiša, Toplak, Ivan, Radojičić, Sonja, "Genska varijabilnost virusa afričke kuge svinja u Srbiji" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):24-28,
https://hdl.handle.net/21.15107/rcub_veterinar_3208 .

TY  - CONF
AU  - Stević, Nataša
AU  - Kosović, Elena
AU  - Radovanović, Tamara
AU  - Radojičić, Sonja
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3209
AB  - Krpeljski encefalitis je veoma značajna zoonoza od koje u Evropi i Aziji godišnje
oboli oko 12000 ljudi. Uzročnik krpeljskog encefalitisa je arbovirus iz familije
Flaviviridae, rod Flavivirus. Postoje tri klasična subtipa virusa krpeljskog encefalitisa
(dalekoistočni, sibirski i evropski), a nedavno su otkrivena još dva (bajkalski i
himalajski). Za uspešnu cirkulaciju i opstanak virusa u prirodi neophodna je
interakcija između vektora i rezervoara. U Evropi oboljenje prenose krpelji vrste
Ixodes ricinus, a u Aziji krpelji vrste Ixodes persulcatus. Glavni rezervoari su mali
glodari, ali i druge životinje. U poslednje vreme je sve više slučajeva oboljenja ljudi
nastalih kao posledica konzumiranja sirovog mleka i mlečnih proizvoda. Bolest kod
ljudi može proticati asimptomatski ili kao oboljenje u kome su ispoljeni neurološki
simptomi različite težine u zavisnosti od subtipa virusa koji je izazvao oboljenje. Mogu
ostati dugotrajne posledice, a u nekim slučajevima može doći i do fatalnog ishoda.
Veliki broj vrsta životinja je prijemčiv, ali se teška klinička slika retko sreće i opisana
je kod pasa, konja, majmuna i veoma retko preživara. Domaći preživari ne ispoljavaju
kliničku sliku, ali izlučuju virus mlekom. Antitela protiv krpeljskog encefalitisa
otkrivena su i kod divljih preživara i karnivora bez specifičnih neuroloških simptoma.
Iako je u pitanju bolest obavezna za prijavljivanje, zbog nedostatka rutinske
dijagnostike, u Srbiji nema podataka o broju slučajeva obolelih ljudi.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Krpeljski encefalitis
SP  - 29
EP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3209
ER  - 

@conference{
author = "Stević, Nataša and Kosović, Elena and Radovanović, Tamara and Radojičić, Sonja",
year = "2023",
abstract = "Krpeljski encefalitis je veoma značajna zoonoza od koje u Evropi i Aziji godišnje
oboli oko 12000 ljudi. Uzročnik krpeljskog encefalitisa je arbovirus iz familije
Flaviviridae, rod Flavivirus. Postoje tri klasična subtipa virusa krpeljskog encefalitisa
(dalekoistočni, sibirski i evropski), a nedavno su otkrivena još dva (bajkalski i
himalajski). Za uspešnu cirkulaciju i opstanak virusa u prirodi neophodna je
interakcija između vektora i rezervoara. U Evropi oboljenje prenose krpelji vrste
Ixodes ricinus, a u Aziji krpelji vrste Ixodes persulcatus. Glavni rezervoari su mali
glodari, ali i druge životinje. U poslednje vreme je sve više slučajeva oboljenja ljudi
nastalih kao posledica konzumiranja sirovog mleka i mlečnih proizvoda. Bolest kod
ljudi može proticati asimptomatski ili kao oboljenje u kome su ispoljeni neurološki
simptomi različite težine u zavisnosti od subtipa virusa koji je izazvao oboljenje. Mogu
ostati dugotrajne posledice, a u nekim slučajevima može doći i do fatalnog ishoda.
Veliki broj vrsta životinja je prijemčiv, ali se teška klinička slika retko sreće i opisana
je kod pasa, konja, majmuna i veoma retko preživara. Domaći preživari ne ispoljavaju
kliničku sliku, ali izlučuju virus mlekom. Antitela protiv krpeljskog encefalitisa
otkrivena su i kod divljih preživara i karnivora bez specifičnih neuroloških simptoma.
Iako je u pitanju bolest obavezna za prijavljivanje, zbog nedostatka rutinske
dijagnostike, u Srbiji nema podataka o broju slučajeva obolelih ljudi.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Krpeljski encefalitis",
pages = "29-34",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3209"
}

Stević, N., Kosović, E., Radovanović, T.,& Radojičić, S.. (2023). Krpeljski encefalitis. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 29-34.
https://hdl.handle.net/21.15107/rcub_veterinar_3209

Stević N, Kosović E, Radovanović T, Radojičić S. Krpeljski encefalitis. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:29-34.
https://hdl.handle.net/21.15107/rcub_veterinar_3209 .

Stević, Nataša, Kosović, Elena, Radovanović, Tamara, Radojičić, Sonja, "Krpeljski encefalitis" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):29-34,
https://hdl.handle.net/21.15107/rcub_veterinar_3209 .

TY  - JOUR
AU  - Sapundžić Zurovac, Zorana
AU  - Žutić, Jadranka
AU  - Stević, Nataša
AU  - Milićević, Vesna
AU  - Radojičić, Marina
AU  - Stanojević, Slavoljub
AU  - Radojičić, Sonja
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2510
AB  - Brucellosis is one of the most important bacterial zoonotic diseases worldwide, characterized in domestic animals by long-term reproductive disorders. As known, wild boars (Sus scrofa) are
natural hosts for Brucella suis biovar 2, in which the infection passes in inapparent form, increasing
the pathogen transmission risk to domestic pigs, other domestic animals and humans. So far, no
studies regarding brucellosis in wild boars in Serbia have been published. During the hunting
season 2020/2021, 480 sera of wild boars living in Serbia were collected and tested for the presence
of anti-Brucella antibodies. For the serological survey, the Rose Bengal Test (RBT) and competitive
enzyme-linked immunosorbent assay (c-ELISA) were used. Of the 480 sera, 45 sera tested positive,
indicating the acquired Brucella seroprevalence in wild boars of 9.4%. The greatest numbers of
Brucella seropositive animals were detected in the eastern parts of the country and in one of the
central districts, i.e., Pomoravski, Branicevski, Borski and Juznobanatski. This study provides the
first data regarding brucellosis in the wild boar population in Serbia, revealing the seroprevalence of
Brucella, thus indicating that wild boars as natural hosts and/or vectors of Brucella likely present a
risk for the infection of other animals.
PB  - MDPI
T2  - Veterinary Sciences
T1  - First Report of Brucella Seroprevalence in Wild Boar Population in Serbia
VL  - 9
IS  - 10
SP  - 575
DO  - 10.3390/vetsci9100575
ER  - 

@article{
author = "Sapundžić Zurovac, Zorana and Žutić, Jadranka and Stević, Nataša and Milićević, Vesna and Radojičić, Marina and Stanojević, Slavoljub and Radojičić, Sonja",
year = "2022",
abstract = "Brucellosis is one of the most important bacterial zoonotic diseases worldwide, characterized in domestic animals by long-term reproductive disorders. As known, wild boars (Sus scrofa) are
natural hosts for Brucella suis biovar 2, in which the infection passes in inapparent form, increasing
the pathogen transmission risk to domestic pigs, other domestic animals and humans. So far, no
studies regarding brucellosis in wild boars in Serbia have been published. During the hunting
season 2020/2021, 480 sera of wild boars living in Serbia were collected and tested for the presence
of anti-Brucella antibodies. For the serological survey, the Rose Bengal Test (RBT) and competitive
enzyme-linked immunosorbent assay (c-ELISA) were used. Of the 480 sera, 45 sera tested positive,
indicating the acquired Brucella seroprevalence in wild boars of 9.4%. The greatest numbers of
Brucella seropositive animals were detected in the eastern parts of the country and in one of the
central districts, i.e., Pomoravski, Branicevski, Borski and Juznobanatski. This study provides the
first data regarding brucellosis in the wild boar population in Serbia, revealing the seroprevalence of
Brucella, thus indicating that wild boars as natural hosts and/or vectors of Brucella likely present a
risk for the infection of other animals.",
publisher = "MDPI",
journal = "Veterinary Sciences",
title = "First Report of Brucella Seroprevalence in Wild Boar Population in Serbia",
volume = "9",
number = "10",
pages = "575",
doi = "10.3390/vetsci9100575"
}

Sapundžić Zurovac, Z., Žutić, J., Stević, N., Milićević, V., Radojičić, M., Stanojević, S.,& Radojičić, S.. (2022). First Report of Brucella Seroprevalence in Wild Boar Population in Serbia. in Veterinary Sciences
MDPI., 9(10), 575.
https://doi.org/10.3390/vetsci9100575

Sapundžić Zurovac Z, Žutić J, Stević N, Milićević V, Radojičić M, Stanojević S, Radojičić S. First Report of Brucella Seroprevalence in Wild Boar Population in Serbia. in Veterinary Sciences. 2022;9(10):575.
doi:10.3390/vetsci9100575 .

Sapundžić Zurovac, Zorana, Žutić, Jadranka, Stević, Nataša, Milićević, Vesna, Radojičić, Marina, Stanojević, Slavoljub, Radojičić, Sonja, "First Report of Brucella Seroprevalence in Wild Boar Population in Serbia" in Veterinary Sciences, 9, no. 10 (2022):575,
https://doi.org/10.3390/vetsci9100575 . .

TY  - JOUR
AU  - Stanojević, Slavoljub
AU  - Radojičić, Sonja
AU  - Mišić, Dušan
AU  - Srejić, Damjan
AU  - Vasiljević, Dragan V.
AU  - Prokić, Kristina
AU  - Ilić, Nevenka
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2630
AB  - It has long been known that coronaviruses cause various infectious diseases in animals. Although SARS-CoV-2 is genetically related to viruses isolated from Rhinolophus bats, the exact origin, mode of transmission, and how the human species has become the epidemiological reservoir of the virus have not yet been established with certainty. Although the main route of transmission is human-to-human, there are considerable numbers of reported cases of infection in animal species, predominantly among pet animals. The aim of this retrospective study was to assess SARS-CoV-2 seropositivity in dogs and cats during the COVID-19 pandemic in Šumadija District, Serbia. We used serology to identify household contacts of pet animals with infected pet owners and the degree of association. The study presented in this paper is also the first study of this type in Serbia. The results of a retrospective serosurvey, which was conducted in dogs and cats with different exposure risk factors, were analyzed to find the possible modes of transmission between humans and animals. The relative frequency of SARS-CoV-2 infection in dogs was 1.45% bounded with a 95% confidence interval (CI) of 0.0007–7.73%, while in cats, it was 5.56% (95% CI: 0.77–4.13%). The relative frequency of SARS-CoV-2 infection in pet owners was 11% (95% CI: 6.25–18.63%). In pets that were in close contact with COVID-19 positive owners, the seropositivity was found to be 9%. Out of a total of five stray dogs and cats tested, seropositivity was observed in two animals. Detected SARS-CoV-2 infection in pets shows that these animals are susceptible to infection and that the most common means of virus transmission to pets is through contact with diseased owners. However, the presence of infection in stray dogs and cats is not clear and needs further research.
PB  - Elsevier B.V.
T2  - Preventive Veterinary Medicine
T1  - Frequency of SARS-CoV-2 infection in dogs and cats: Results of a retrospective serological survey in Šumadija District, Serbia
VL  - 208
DO  - 10.1016/j.prevetmed.2022.105755
ER  - 

@article{
author = "Stanojević, Slavoljub and Radojičić, Sonja and Mišić, Dušan and Srejić, Damjan and Vasiljević, Dragan V. and Prokić, Kristina and Ilić, Nevenka",
year = "2022",
abstract = "It has long been known that coronaviruses cause various infectious diseases in animals. Although SARS-CoV-2 is genetically related to viruses isolated from Rhinolophus bats, the exact origin, mode of transmission, and how the human species has become the epidemiological reservoir of the virus have not yet been established with certainty. Although the main route of transmission is human-to-human, there are considerable numbers of reported cases of infection in animal species, predominantly among pet animals. The aim of this retrospective study was to assess SARS-CoV-2 seropositivity in dogs and cats during the COVID-19 pandemic in Šumadija District, Serbia. We used serology to identify household contacts of pet animals with infected pet owners and the degree of association. The study presented in this paper is also the first study of this type in Serbia. The results of a retrospective serosurvey, which was conducted in dogs and cats with different exposure risk factors, were analyzed to find the possible modes of transmission between humans and animals. The relative frequency of SARS-CoV-2 infection in dogs was 1.45% bounded with a 95% confidence interval (CI) of 0.0007–7.73%, while in cats, it was 5.56% (95% CI: 0.77–4.13%). The relative frequency of SARS-CoV-2 infection in pet owners was 11% (95% CI: 6.25–18.63%). In pets that were in close contact with COVID-19 positive owners, the seropositivity was found to be 9%. Out of a total of five stray dogs and cats tested, seropositivity was observed in two animals. Detected SARS-CoV-2 infection in pets shows that these animals are susceptible to infection and that the most common means of virus transmission to pets is through contact with diseased owners. However, the presence of infection in stray dogs and cats is not clear and needs further research.",
publisher = "Elsevier B.V.",
journal = "Preventive Veterinary Medicine",
title = "Frequency of SARS-CoV-2 infection in dogs and cats: Results of a retrospective serological survey in Šumadija District, Serbia",
volume = "208",
doi = "10.1016/j.prevetmed.2022.105755"
}

Stanojević, S., Radojičić, S., Mišić, D., Srejić, D., Vasiljević, D. V., Prokić, K.,& Ilić, N.. (2022). Frequency of SARS-CoV-2 infection in dogs and cats: Results of a retrospective serological survey in Šumadija District, Serbia. in Preventive Veterinary Medicine
Elsevier B.V.., 208.
https://doi.org/10.1016/j.prevetmed.2022.105755

Stanojević S, Radojičić S, Mišić D, Srejić D, Vasiljević DV, Prokić K, Ilić N. Frequency of SARS-CoV-2 infection in dogs and cats: Results of a retrospective serological survey in Šumadija District, Serbia. in Preventive Veterinary Medicine. 2022;208.
doi:10.1016/j.prevetmed.2022.105755 .

Stanojević, Slavoljub, Radojičić, Sonja, Mišić, Dušan, Srejić, Damjan, Vasiljević, Dragan V., Prokić, Kristina, Ilić, Nevenka, "Frequency of SARS-CoV-2 infection in dogs and cats: Results of a retrospective serological survey in Šumadija District, Serbia" in Preventive Veterinary Medicine, 208 (2022),
https://doi.org/10.1016/j.prevetmed.2022.105755 . .

TY  - CONF
AU  - Valčić, Miroslav
AU  - Radojičić, Sonja
AU  - Stević, Nataša
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3196
AB  - Epizootiološke determinante predstavljaju osnov za poznavanje epizootioloških
karakteristika pojedinih zaraznih i parazitskih oboljenja, kao i poremećaje
zdravlja nezarazne i neparazitske etiologije. U osnovi, radi se o tri determiante,
a to su:
1. Prijemčiva vrsta,
2. Uzročni faktor i
3. Spoljašnji faktor.
Region Srbije u kome se nalazi Stara planina je izuzetno interesantan sa epizootiološkog
aspekta. To se naročito odnosi na površinu koju zahvata opština
Dimitrovgad, koja obuhvata delove Stare planine i urbanu sredinu, u čijoj neposrednoj
blizini se nalazi jedan od najznačajnijih puteva koji spajaju Evropu
sa Istokom kao i na bogatstvo flore i faune kakvo se retko gde sreće u regionu.
U odnosu na prijemčive vrste, radi se o regionu u kome se nalazi veći broj vrsta
domaćih životinja čija tehnologija uzgoja značajno varira. U odnosu na uzročni
faktor, preliminarna ispitivanja su dokazala da na izgled izolovan region, može
da predstavlja značajan rezervoar mikroorganizama koji mogu da izazovu
epizootije. Istovremeno, a u sadejstvu sa spoljašnjim faktorom, naročito prometom
između regiona sveta gde se još uvek pojavljuju egzotične zarazne bolesti
životinja, postoji rizik od unosa uzročnika zaraznih bolesti koje se smatraju egzotičnim
za našu zemlju i Evropu. Raznovrsnost i variranje elemenata spoljašnje sredine kao epizootiološkog faktora,
čine da je procena epizootiološke situacije u regionu Stare planine teška,
a analiza rizika od pojave epizootija relativno kompleksna.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021
T1  - Epizootiološke determinante regiona Stara planina (Srbija) i njihov uticaj na procenu rizika od pojave epizootija
SP  - 271
EP  - 283
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3196
ER  - 

@conference{
author = "Valčić, Miroslav and Radojičić, Sonja and Stević, Nataša",
year = "2021",
abstract = "Epizootiološke determinante predstavljaju osnov za poznavanje epizootioloških
karakteristika pojedinih zaraznih i parazitskih oboljenja, kao i poremećaje
zdravlja nezarazne i neparazitske etiologije. U osnovi, radi se o tri determiante,
a to su:
1. Prijemčiva vrsta,
2. Uzročni faktor i
3. Spoljašnji faktor.
Region Srbije u kome se nalazi Stara planina je izuzetno interesantan sa epizootiološkog
aspekta. To se naročito odnosi na površinu koju zahvata opština
Dimitrovgad, koja obuhvata delove Stare planine i urbanu sredinu, u čijoj neposrednoj
blizini se nalazi jedan od najznačajnijih puteva koji spajaju Evropu
sa Istokom kao i na bogatstvo flore i faune kakvo se retko gde sreće u regionu.
U odnosu na prijemčive vrste, radi se o regionu u kome se nalazi veći broj vrsta
domaćih životinja čija tehnologija uzgoja značajno varira. U odnosu na uzročni
faktor, preliminarna ispitivanja su dokazala da na izgled izolovan region, može
da predstavlja značajan rezervoar mikroorganizama koji mogu da izazovu
epizootije. Istovremeno, a u sadejstvu sa spoljašnjim faktorom, naročito prometom
između regiona sveta gde se još uvek pojavljuju egzotične zarazne bolesti
životinja, postoji rizik od unosa uzročnika zaraznih bolesti koje se smatraju egzotičnim
za našu zemlju i Evropu. Raznovrsnost i variranje elemenata spoljašnje sredine kao epizootiološkog faktora,
čine da je procena epizootiološke situacije u regionu Stare planine teška,
a analiza rizika od pojave epizootija relativno kompleksna.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021",
title = "Epizootiološke determinante regiona Stara planina (Srbija) i njihov uticaj na procenu rizika od pojave epizootija",
pages = "271-283",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3196"
}

Valčić, M., Radojičić, S.,& Stević, N.. (2021). Epizootiološke determinante regiona Stara planina (Srbija) i njihov uticaj na procenu rizika od pojave epizootija. in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021
Beograd : Srpsko veterinarsko društvo., 271-283.
https://hdl.handle.net/21.15107/rcub_veterinar_3196

Valčić M, Radojičić S, Stević N. Epizootiološke determinante regiona Stara planina (Srbija) i njihov uticaj na procenu rizika od pojave epizootija. in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021. 2021;:271-283.
https://hdl.handle.net/21.15107/rcub_veterinar_3196 .

Valčić, Miroslav, Radojičić, Sonja, Stević, Nataša, "Epizootiološke determinante regiona Stara planina (Srbija) i njihov uticaj na procenu rizika od pojave epizootija" in Treći simpozijum Zaštita agrobiodiverziteta i očuvanje autohtonih rasa domaćih životinja, Dimitrovgrad, 25 - 27. jun 2021 (2021):271-283,
https://hdl.handle.net/21.15107/rcub_veterinar_3196 .

TY  - CONF
AU  - Radojičić, Sonja
AU  - Stević, Nataša
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3372
AB  - Kao jedno od najvećih dostignuća savremene medicine, vakcinacija danas
prevazilazi svoj prvobitni koncept zaštite životinja i ljudi od infektivnih bolesti. Hvaljena i osporavana, vakcinacija predstavlja najvažniju preventivnu meru kojom se
svakodnevno spašavaju životi ljudi i životinja. Jedno od novih polja u kojima bi
imunološki preparati mogli da preuzmu primat je kontrola reproduktivnog statusa
životinja. Delujući u smislu povećanja reproduktivnih performansi ili u smeru kontracepcije, vakcine omogućavaju jednostavniju i lakšu manipulaciju, kao i humaniju kontrolu brojnosti određenih populacija životinja. Takozvane ‘’kontraceptivne
vakcine’’ omogućavaju neinvazivnu sterilizaciju kućnih ljubimaca, pasa i mačaka
lutalica, ali upotreba ovakvih vakcina može da nađe i primenu u terapiji različitih
poremećaja u kojima je neophodna kontrola lučenja polnih hormona kao što su
sindrom policističnih jajnika ili endometrioza. Sa druge strane, uz pomoć vakcina kojima se povećava fertilitet životinja koje se gaje za ishranu ljudi, mogu se
ostvariti značajni ekonomski dobici i izbeći upotreba hormona poštujući koncepte
‘’Jedinstvenog zdravlja’’. Imajući u vidu ogromne potencijale koje će biološki preparati namenjeni za kontrolu reprodukcije, ali i terapiju određenih kliničkih slučajeva, imati u budućnosti, neophodno je naglasiti potrebu rigorozne kontrole upotrebe ovakvih preparata u cilju sprečavanja širenja dezinformacija i mogućih zloupotreba.
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
C3  - 12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 7 - 10. oktobar 2021
T1  - Razvoj i primena imunoloških preparata u kontroli reproduktivnih performasi životinja
SP  - 19
EP  - 25
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3372
ER  - 

@conference{
author = "Radojičić, Sonja and Stević, Nataša",
year = "2021",
abstract = "Kao jedno od najvećih dostignuća savremene medicine, vakcinacija danas
prevazilazi svoj prvobitni koncept zaštite životinja i ljudi od infektivnih bolesti. Hvaljena i osporavana, vakcinacija predstavlja najvažniju preventivnu meru kojom se
svakodnevno spašavaju životi ljudi i životinja. Jedno od novih polja u kojima bi
imunološki preparati mogli da preuzmu primat je kontrola reproduktivnog statusa
životinja. Delujući u smislu povećanja reproduktivnih performansi ili u smeru kontracepcije, vakcine omogućavaju jednostavniju i lakšu manipulaciju, kao i humaniju kontrolu brojnosti određenih populacija životinja. Takozvane ‘’kontraceptivne
vakcine’’ omogućavaju neinvazivnu sterilizaciju kućnih ljubimaca, pasa i mačaka
lutalica, ali upotreba ovakvih vakcina može da nađe i primenu u terapiji različitih
poremećaja u kojima je neophodna kontrola lučenja polnih hormona kao što su
sindrom policističnih jajnika ili endometrioza. Sa druge strane, uz pomoć vakcina kojima se povećava fertilitet životinja koje se gaje za ishranu ljudi, mogu se
ostvariti značajni ekonomski dobici i izbeći upotreba hormona poštujući koncepte
‘’Jedinstvenog zdravlja’’. Imajući u vidu ogromne potencijale koje će biološki preparati namenjeni za kontrolu reprodukcije, ali i terapiju određenih kliničkih slučajeva, imati u budućnosti, neophodno je naglasiti potrebu rigorozne kontrole upotrebe ovakvih preparata u cilju sprečavanja širenja dezinformacija i mogućih zloupotreba.",
publisher = "Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 7 - 10. oktobar 2021",
title = "Razvoj i primena imunoloških preparata u kontroli reproduktivnih performasi životinja",
pages = "19-25",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3372"
}

Radojičić, S.,& Stević, N.. (2021). Razvoj i primena imunoloških preparata u kontroli reproduktivnih performasi životinja. in 12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 7 - 10. oktobar 2021
Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila., 19-25.
https://hdl.handle.net/21.15107/rcub_veterinar_3372

Radojičić S, Stević N. Razvoj i primena imunoloških preparata u kontroli reproduktivnih performasi životinja. in 12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 7 - 10. oktobar 2021. 2021;:19-25.
https://hdl.handle.net/21.15107/rcub_veterinar_3372 .

Radojičić, Sonja, Stević, Nataša, "Razvoj i primena imunoloških preparata u kontroli reproduktivnih performasi životinja" in 12. Naučni simpozijum Reprodukcija domaćih životinja, Divčibare, 7 - 10. oktobar 2021 (2021):19-25,
https://hdl.handle.net/21.15107/rcub_veterinar_3372 .

TY  - JOUR
AU  - Stanojević, Slavoljub
AU  - Ponjavić, Mirza
AU  - Stanojević, Slobodan
AU  - Stevanović, Aleksandar
AU  - Radojičić, Sonja
PY  - 2021
AB  - As a response to the pandemic caused by SARS-Cov-2 virus, on 15 March 2020, the Republic of Serbia introduced comprehensive anti-epidemic measures to curb COVID-19. After a slowdown in the epidemic, on 6 May 2020, the regulatory authorities decided to relax the implemented measures. However, the epidemiological situation soon worsened again. As of 7 February 2021, a total of 406,352 cases of SARSCov-2 infection have been reported in Serbia, 4,112 deaths caused by COVID-19. In order to better understand the epidemic dynamics and predict possible outcomes, we have developed an adaptive mathematical model SEAIHRDS (S-susceptible, E-exposed, A-asymptomatic, I-infected, H-hospitalized, R-recovered, d-dead due to COVID-19 infection, S-susceptible). The model can be used to simulate various scenarios of the implemented intervention measures and calculate possible epidemic outcomes, including the necessary hospital capacities. Considering promising results regarding the development of a vaccine against COVID-19, the model is extended to simulate vaccination among different population strata. The findings from various simulation scenarios have shown that, with implementation of strict measures of contact reduction, it is possible to control COVID-19 and reduce number of deaths. The findings also show that limiting effective contacts within the most susceptible population strata merits a special attention. However, the findings also show that the disease has a potential to remain in the population for a long time, likely with a seasonal pattern. If a vaccine, with efficacy equal or higher than 65%, becomes available it could help to significantly slow down or completely stop circulation of the virus in human population. The effects of vaccination depend primarily on: 1. Efficacy of available vaccine(s), 2. Prioritization of the population categories for vaccination, and 3. Overall vaccination coverage of the population, assuming that the vaccine(s) develop solid immunity in vaccinated individuals. With expected basic reproduction number of Ro=2.46 and vaccine efficacy of 68%, an 87% coverage would be sufficient to stop the virus circulation.
PB  - Elsevier
T2  - Microbial Risk Analysis
T1  - Simulation and prediction of spread of COVID-19 in The Republic of Serbia by SEAIHRDS model of disease transmission
SP  - 100161
DO  - 10.1016/j.mran.2021.100161
ER  - 

@article{
author = "Stanojević, Slavoljub and Ponjavić, Mirza and Stanojević, Slobodan and Stevanović, Aleksandar and Radojičić, Sonja",
year = "2021",
abstract = "As a response to the pandemic caused by SARS-Cov-2 virus, on 15 March 2020, the Republic of Serbia introduced comprehensive anti-epidemic measures to curb COVID-19. After a slowdown in the epidemic, on 6 May 2020, the regulatory authorities decided to relax the implemented measures. However, the epidemiological situation soon worsened again. As of 7 February 2021, a total of 406,352 cases of SARSCov-2 infection have been reported in Serbia, 4,112 deaths caused by COVID-19. In order to better understand the epidemic dynamics and predict possible outcomes, we have developed an adaptive mathematical model SEAIHRDS (S-susceptible, E-exposed, A-asymptomatic, I-infected, H-hospitalized, R-recovered, d-dead due to COVID-19 infection, S-susceptible). The model can be used to simulate various scenarios of the implemented intervention measures and calculate possible epidemic outcomes, including the necessary hospital capacities. Considering promising results regarding the development of a vaccine against COVID-19, the model is extended to simulate vaccination among different population strata. The findings from various simulation scenarios have shown that, with implementation of strict measures of contact reduction, it is possible to control COVID-19 and reduce number of deaths. The findings also show that limiting effective contacts within the most susceptible population strata merits a special attention. However, the findings also show that the disease has a potential to remain in the population for a long time, likely with a seasonal pattern. If a vaccine, with efficacy equal or higher than 65%, becomes available it could help to significantly slow down or completely stop circulation of the virus in human population. The effects of vaccination depend primarily on: 1. Efficacy of available vaccine(s), 2. Prioritization of the population categories for vaccination, and 3. Overall vaccination coverage of the population, assuming that the vaccine(s) develop solid immunity in vaccinated individuals. With expected basic reproduction number of Ro=2.46 and vaccine efficacy of 68%, an 87% coverage would be sufficient to stop the virus circulation.",
publisher = "Elsevier",
journal = "Microbial Risk Analysis",
title = "Simulation and prediction of spread of COVID-19 in The Republic of Serbia by SEAIHRDS model of disease transmission",
pages = "100161",
doi = "10.1016/j.mran.2021.100161"
}

Stanojević, S., Ponjavić, M., Stanojević, S., Stevanović, A.,& Radojičić, S.. (2021). Simulation and prediction of spread of COVID-19 in The Republic of Serbia by SEAIHRDS model of disease transmission. in Microbial Risk Analysis
Elsevier., 100161.
https://doi.org/10.1016/j.mran.2021.100161

Stanojević S, Ponjavić M, Stanojević S, Stevanović A, Radojičić S. Simulation and prediction of spread of COVID-19 in The Republic of Serbia by SEAIHRDS model of disease transmission. in Microbial Risk Analysis. 2021;:100161.
doi:10.1016/j.mran.2021.100161 .

Stanojević, Slavoljub, Ponjavić, Mirza, Stanojević, Slobodan, Stevanović, Aleksandar, Radojičić, Sonja, "Simulation and prediction of spread of COVID-19 in The Republic of Serbia by SEAIHRDS model of disease transmission" in Microbial Risk Analysis (2021):100161,
https://doi.org/10.1016/j.mran.2021.100161 . .

TY  - JOUR
AU  - Radojičić, Sonja
AU  - Živulj, Aleksandar
AU  - Petrovic, Tamas
AU  - Nišavić, Jakov
AU  - Milićević, Vesna
AU  - Sipetic-Grujicic, Sandra
AU  - Mišić, Dusan
AU  - Korzeniowska, Malgorzata
AU  - Stanojević, Slavoljub
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2298
AB  - West Nile virus (WNV) is an arthropod-born pathogen, which is transmitted from wild birds through mosquitoes to humans and animals. At the end of the 20th century, the first West Nile fever (WNF) outbreaks among humans in urban environments in Eastern Europe and the United States were reported. The disease continued to spread to other parts of the continents. In Serbia, the largest number of WNV-infected people was recorded in 2018. This research used spatial statistics to identify clusters of WNV infection in humans and animals in South Banat County, Serbia. The occurrence of WNV infection and risk factors were analyzed using a negative binomial regression model. Our research indicated that climatic factors were the main determinant of WNV distribution and were predictors of endemicity. Precipitation and water levels of rivers had an important influence on mosquito abundance and affected the habitats of wild birds, which are important for maintaining the virus in nature. We found that the maximum temperature of the warmest part of the year and the annual temperature range; and hydrographic variables, e.g., the presence of rivers and water streams were the best environmental predictors of WNF outbreaks in South Banat County.
PB  - MDPI
T2  - Animals
T1  - Spatiotemporal Analysis of West Nile Virus Epidemic in South Banat District, Serbia, 2017–2019
VL  - 11
IS  - 10
SP  - 2951
DO  - 10.3390/ani11102951
ER  - 

@article{
author = "Radojičić, Sonja and Živulj, Aleksandar and Petrovic, Tamas and Nišavić, Jakov and Milićević, Vesna and Sipetic-Grujicic, Sandra and Mišić, Dusan and Korzeniowska, Malgorzata and Stanojević, Slavoljub",
year = "2021",
abstract = "West Nile virus (WNV) is an arthropod-born pathogen, which is transmitted from wild birds through mosquitoes to humans and animals. At the end of the 20th century, the first West Nile fever (WNF) outbreaks among humans in urban environments in Eastern Europe and the United States were reported. The disease continued to spread to other parts of the continents. In Serbia, the largest number of WNV-infected people was recorded in 2018. This research used spatial statistics to identify clusters of WNV infection in humans and animals in South Banat County, Serbia. The occurrence of WNV infection and risk factors were analyzed using a negative binomial regression model. Our research indicated that climatic factors were the main determinant of WNV distribution and were predictors of endemicity. Precipitation and water levels of rivers had an important influence on mosquito abundance and affected the habitats of wild birds, which are important for maintaining the virus in nature. We found that the maximum temperature of the warmest part of the year and the annual temperature range; and hydrographic variables, e.g., the presence of rivers and water streams were the best environmental predictors of WNF outbreaks in South Banat County.",
publisher = "MDPI",
journal = "Animals",
title = "Spatiotemporal Analysis of West Nile Virus Epidemic in South Banat District, Serbia, 2017–2019",
volume = "11",
number = "10",
pages = "2951",
doi = "10.3390/ani11102951"
}

Radojičić, S., Živulj, A., Petrovic, T., Nišavić, J., Milićević, V., Sipetic-Grujicic, S., Mišić, D., Korzeniowska, M.,& Stanojević, S.. (2021). Spatiotemporal Analysis of West Nile Virus Epidemic in South Banat District, Serbia, 2017–2019. in Animals
MDPI., 11(10), 2951.
https://doi.org/10.3390/ani11102951

Radojičić S, Živulj A, Petrovic T, Nišavić J, Milićević V, Sipetic-Grujicic S, Mišić D, Korzeniowska M, Stanojević S. Spatiotemporal Analysis of West Nile Virus Epidemic in South Banat District, Serbia, 2017–2019. in Animals. 2021;11(10):2951.
doi:10.3390/ani11102951 .

Radojičić, Sonja, Živulj, Aleksandar, Petrovic, Tamas, Nišavić, Jakov, Milićević, Vesna, Sipetic-Grujicic, Sandra, Mišić, Dusan, Korzeniowska, Malgorzata, Stanojević, Slavoljub, "Spatiotemporal Analysis of West Nile Virus Epidemic in South Banat District, Serbia, 2017–2019" in Animals, 11, no. 10 (2021):2951,
https://doi.org/10.3390/ani11102951 . .

TY  - JOUR
AU  - Milićević, Vesna
AU  - Kureljušić, Branislav
AU  - Veljović, Ljubiša
AU  - Valčić, Miroslav
AU  - Stević, Nataša
AU  - Savić, Božidar
AU  - Radojičić, Sonja
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1793
AB  - Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The performance of seven molecular methods for the detection of prrsv
VL  - 70
IS  - 1
SP  - 51
EP  - 57
DO  - 10.2478/acve-2020-0003
ER  - 

@article{
author = "Milićević, Vesna and Kureljušić, Branislav and Veljović, Ljubiša and Valčić, Miroslav and Stević, Nataša and Savić, Božidar and Radojičić, Sonja",
year = "2020",
abstract = "Porcine Reproductive and Respiratory Syndrome is a viral disease of swine characterized by reproductive failure of breeding animals and respiratory disorders in all categories. The first PRRS case in Serbia was recorded in 2001 after illegal import of boar semen. PRRS is economically the most important disease due to significant direct and indirect losses. Today, for routine diagnosis of PRRS in infected herds serological methods (ELISA) and molecular methods are used. Although modern diagnostic techniques are very robust, exceptional diversity of the viral strains is often the obstacle for an accurate diagnosis. To estimate the performance of seven different methods for PRRSV genome detection, twenty samples were used. However, none of the methods was able to detect all PRRSV strains. The best sensitivity was obtained by combining two methods. Until today, there is no absolutely accurate test which enables the detection of all circulating strains.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The performance of seven molecular methods for the detection of prrsv",
volume = "70",
number = "1",
pages = "51-57",
doi = "10.2478/acve-2020-0003"
}

Milićević, V., Kureljušić, B., Veljović, L., Valčić, M., Stević, N., Savić, B.,& Radojičić, S.. (2020). The performance of seven molecular methods for the detection of prrsv. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(1), 51-57.
https://doi.org/10.2478/acve-2020-0003

Milićević V, Kureljušić B, Veljović L, Valčić M, Stević N, Savić B, Radojičić S. The performance of seven molecular methods for the detection of prrsv. in Acta Veterinaria-Beograd. 2020;70(1):51-57.
doi:10.2478/acve-2020-0003 .

Milićević, Vesna, Kureljušić, Branislav, Veljović, Ljubiša, Valčić, Miroslav, Stević, Nataša, Savić, Božidar, Radojičić, Sonja, "The performance of seven molecular methods for the detection of prrsv" in Acta Veterinaria-Beograd, 70, no. 1 (2020):51-57,
https://doi.org/10.2478/acve-2020-0003 . .

TY  - GEN
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav,
AU  - Stević, Nataša
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2632
AB  - Poslednjih godina je evidentno pogoršanje epizootiološke situacije naročito opasnih zaraznih bolesti, ne samo u Republici Srbiji i regionu Balkanskog poluostrva već i u celom svetu. Afrička kuga svinja
postepeno, ali sigurno osvaja evropski kontinent pa su u 2019. godini
Slovačka i Srbija po prvi put zabeležile pojavu ove bolesti. Sa druge
strane, neprekidno prisustvo slinavke i šapa, boginja ovaca i boginja
koza, kao i širenje kuge malih preživara u Turskoj i van njenih granica,
pokazatelji su sve većih zahteva za dobrom organizacijom veterinarske struke naše zemlje. Uz bolest plavog jezika, nodularni dermatitis,
afričku kugu svinja i bolest Zapadnog Nila, veterinari su suočeni sa
pojavom sve većeg broja novih zaraznih bolesti za koje su potrebna
znanja kako bi se one na vreme prepoznale i njihovo širenje zaustavilo. Evidentan je porast broja slučajeva tuberkuloze, bruceloze i drugih
bolesti za koje se verovalo da su potpuno pod kontrolom. Veterinari
su pod stalnim pritiskom pojave ne samo naročito opasnih zaraznih
bolesti koje prete regionu, već i onih za koje se verovalo da su na pragu iskorenjivanja. Izbijanje jedne od najopasnijih zoonoza, groznice
doline Rift na francuskom ostrvu Majote, važnoj turističkoj destinaciji,
ukazuje na to da ne treba zanemariti ni rizik širenja bolesti uveženim
slučajevima, ne samo kod ljudi, već i kod životinja.
AB  - EMERGING DISEASES SIGNIFICANT FOR SERBIA AND THE REGION
Over the last few years, there has been an apparent deterioration of epizootiological
situation especially in case of emerging diseases, not only in the Republic of Serbia and
the Balkan peninsula region, but also worldwide. African swine fever has been gradually
but surely spreading across Europe. The disease was recorded for the first time in Serbia
22 XLI INOVACIJE ZNANJA VETERINARA
Beograd, 2020
and Slovakia, in 2019. On the other hand, continuous presence of foot and mouth disease,
capripox and spreading of peste des petit ruminants in and out of Turkey, clearly indicates
the importance of good organisation of veterinary profession in our country. Along with blue
tongue disease, nodular dermatitis, African swine fever, West Nile fever, veterinarians are
facing increasing number of new contagious diseases which requires knowledge to detect
them on time and prevent their spreading. Cases of tuberculosis, brucelosis and other
diseases, that have been assumed as kept under control, have significantly increased.
Veterinarians are under constant pressure not only by a potential outbreak of emerging
diseases that might impose the threat to the region, but also of those considered to have
been eradicated. Outbreak of one of the most dangerous zoonosis, Rift Valley fever on
the French island of Majote, an important touristic destination, shows that we should not
neglect the risk of spreading the disease by imported cases, not only in humans but also
in animals.
PB  - Fakultet veterinarske medicine, Beograd Centar za izdavačku delatnost i promet učila
PB  - Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila
T2  - XLI SEMINAR ZA INOVACIJE ZNANJA VETERINARA
T1  - Naročito opasne zarazne bolesti životinja od značaja za Srbiju i region
SP  - 11
EP  - 22
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2632
ER  - 

@misc{
author = "Radojičić, Sonja and Valčić, Miroslav, and Stević, Nataša",
year = "2020",
abstract = "Poslednjih godina je evidentno pogoršanje epizootiološke situacije naročito opasnih zaraznih bolesti, ne samo u Republici Srbiji i regionu Balkanskog poluostrva već i u celom svetu. Afrička kuga svinja
postepeno, ali sigurno osvaja evropski kontinent pa su u 2019. godini
Slovačka i Srbija po prvi put zabeležile pojavu ove bolesti. Sa druge
strane, neprekidno prisustvo slinavke i šapa, boginja ovaca i boginja
koza, kao i širenje kuge malih preživara u Turskoj i van njenih granica,
pokazatelji su sve većih zahteva za dobrom organizacijom veterinarske struke naše zemlje. Uz bolest plavog jezika, nodularni dermatitis,
afričku kugu svinja i bolest Zapadnog Nila, veterinari su suočeni sa
pojavom sve većeg broja novih zaraznih bolesti za koje su potrebna
znanja kako bi se one na vreme prepoznale i njihovo širenje zaustavilo. Evidentan je porast broja slučajeva tuberkuloze, bruceloze i drugih
bolesti za koje se verovalo da su potpuno pod kontrolom. Veterinari
su pod stalnim pritiskom pojave ne samo naročito opasnih zaraznih
bolesti koje prete regionu, već i onih za koje se verovalo da su na pragu iskorenjivanja. Izbijanje jedne od najopasnijih zoonoza, groznice
doline Rift na francuskom ostrvu Majote, važnoj turističkoj destinaciji,
ukazuje na to da ne treba zanemariti ni rizik širenja bolesti uveženim
slučajevima, ne samo kod ljudi, već i kod životinja., EMERGING DISEASES SIGNIFICANT FOR SERBIA AND THE REGION
Over the last few years, there has been an apparent deterioration of epizootiological
situation especially in case of emerging diseases, not only in the Republic of Serbia and
the Balkan peninsula region, but also worldwide. African swine fever has been gradually
but surely spreading across Europe. The disease was recorded for the first time in Serbia
22 XLI INOVACIJE ZNANJA VETERINARA
Beograd, 2020
and Slovakia, in 2019. On the other hand, continuous presence of foot and mouth disease,
capripox and spreading of peste des petit ruminants in and out of Turkey, clearly indicates
the importance of good organisation of veterinary profession in our country. Along with blue
tongue disease, nodular dermatitis, African swine fever, West Nile fever, veterinarians are
facing increasing number of new contagious diseases which requires knowledge to detect
them on time and prevent their spreading. Cases of tuberculosis, brucelosis and other
diseases, that have been assumed as kept under control, have significantly increased.
Veterinarians are under constant pressure not only by a potential outbreak of emerging
diseases that might impose the threat to the region, but also of those considered to have
been eradicated. Outbreak of one of the most dangerous zoonosis, Rift Valley fever on
the French island of Majote, an important touristic destination, shows that we should not
neglect the risk of spreading the disease by imported cases, not only in humans but also
in animals.",
publisher = "Fakultet veterinarske medicine, Beograd Centar za izdavačku delatnost i promet učila, Beograd : Fakultet veterinarske medicine, Centar za izdavačku delatnost i promet učila",
journal = "XLI SEMINAR ZA INOVACIJE ZNANJA VETERINARA",
title = "Naročito opasne zarazne bolesti životinja od značaja za Srbiju i region",
pages = "11-22",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2632"
}

Radojičić, S., Valčić, M.,& Stević, N.. (2020). Naročito opasne zarazne bolesti životinja od značaja za Srbiju i region. in XLI SEMINAR ZA INOVACIJE ZNANJA VETERINARA
Fakultet veterinarske medicine, Beograd Centar za izdavačku delatnost i promet učila., 11-22.
https://hdl.handle.net/21.15107/rcub_veterinar_2632

Radojičić S, Valčić M, Stević N. Naročito opasne zarazne bolesti životinja od značaja za Srbiju i region. in XLI SEMINAR ZA INOVACIJE ZNANJA VETERINARA. 2020;:11-22.
https://hdl.handle.net/21.15107/rcub_veterinar_2632 .

Radojičić, Sonja, Valčić, Miroslav,, Stević, Nataša, "Naročito opasne zarazne bolesti životinja od značaja za Srbiju i region" in XLI SEMINAR ZA INOVACIJE ZNANJA VETERINARA (2020):11-22,
https://hdl.handle.net/21.15107/rcub_veterinar_2632 .

TY  - JOUR
AU  - Milovanović, Milovan
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Hoffmann, Bernd
AU  - Dietze, Klaas
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1795
AB  - Background The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive sampling. Methods Samples for this study were collected from dairy cows vaccinated against LSD in an area without reported LSD virus circulation. Paired serum and milk (individual and bulk) samples were tested by ELISA without and with modifications of the sample incubation time for the milk samples. For the evaluation of the test specificity, 352 milk samples from a milk repository in Germany were used as negative control. Receiver operating characteristic analysis was performed for determination of the Youden index and determination of the most suitable cut-off value for maximum specificity. Results From 154 analyzed serum samples from Serbia, 75 were detected as positive in the ELISA. Sensitivity and specificity of the ELISA test for milk samples reached values of 88 to 91% using Youden criteria. A cut-off of 10 was determined aiming for maximum specificity. This cut-off value was used for further analysis. Using the protocol for serum, out of 154 milk samples, 38 were detected as positive, number of positive detected milk samples increase up to 48 with modified protocol. Milk samples from Germany reacted negative, except two samples that had borderline results using modified protocol. Significant statistical difference (p < 0.05) was observed between two incubation protocols. The detection of LSD-specific antibodies from bulk milk samples (pools of 2-10 individuals) came along with a reduced sensitivity over the sample of individual animals. Conclusions Results show that the detection of capripoxvirus specific antibodies in milk samples using the commercially available ELISA from IDvet is feasible and can represent a helpful tool for LSDV monitoring programs.
PB  - BMC, London
T2  - Virology Journal
T1  - Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA
VL  - 17
IS  - 1
SP  - 28
DO  - 10.1186/s12985-020-01298-x
ER  - 

@article{
author = "Milovanović, Milovan and Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Hoffmann, Bernd and Dietze, Klaas",
year = "2020",
abstract = "Background The detection of antibodies against capripoxvirus has become easier with a commercially available ELISA validated for serum and plasma. In order to explore its suitability for immunological investigations on alternative samples, this study targeted milk as sample matrix available through non-invasive sampling. Methods Samples for this study were collected from dairy cows vaccinated against LSD in an area without reported LSD virus circulation. Paired serum and milk (individual and bulk) samples were tested by ELISA without and with modifications of the sample incubation time for the milk samples. For the evaluation of the test specificity, 352 milk samples from a milk repository in Germany were used as negative control. Receiver operating characteristic analysis was performed for determination of the Youden index and determination of the most suitable cut-off value for maximum specificity. Results From 154 analyzed serum samples from Serbia, 75 were detected as positive in the ELISA. Sensitivity and specificity of the ELISA test for milk samples reached values of 88 to 91% using Youden criteria. A cut-off of 10 was determined aiming for maximum specificity. This cut-off value was used for further analysis. Using the protocol for serum, out of 154 milk samples, 38 were detected as positive, number of positive detected milk samples increase up to 48 with modified protocol. Milk samples from Germany reacted negative, except two samples that had borderline results using modified protocol. Significant statistical difference (p < 0.05) was observed between two incubation protocols. The detection of LSD-specific antibodies from bulk milk samples (pools of 2-10 individuals) came along with a reduced sensitivity over the sample of individual animals. Conclusions Results show that the detection of capripoxvirus specific antibodies in milk samples using the commercially available ELISA from IDvet is feasible and can represent a helpful tool for LSDV monitoring programs.",
publisher = "BMC, London",
journal = "Virology Journal",
title = "Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA",
volume = "17",
number = "1",
pages = "28",
doi = "10.1186/s12985-020-01298-x"
}

Milovanović, M., Milićević, V., Radojičić, S., Valčić, M., Hoffmann, B.,& Dietze, K.. (2020). Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA. in Virology Journal
BMC, London., 17(1), 28.
https://doi.org/10.1186/s12985-020-01298-x

Milovanović M, Milićević V, Radojičić S, Valčić M, Hoffmann B, Dietze K. Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA. in Virology Journal. 2020;17(1):28.
doi:10.1186/s12985-020-01298-x .

Milovanović, Milovan, Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Hoffmann, Bernd, Dietze, Klaas, "Suitability of individual and bulk milk samples to investigate the humoral immune response to lumpy skin disease vaccination by ELISA" in Virology Journal, 17, no. 1 (2020):28,
https://doi.org/10.1186/s12985-020-01298-x . .

TY  - JOUR
AU  - Matović, Kazimir
AU  - Vidanović, Dejan
AU  - Manić, Marija
AU  - Stojiljković, Marko
AU  - Radojičić, Sonja
AU  - Debeljak, Zoran
AU  - Šekler, Milanko
AU  - Ćirić, Jelena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1797
AB  - A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992-2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009-2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection.
PB  - Elsevier, Amsterdam
T2  - Saudi Journal of Biological Sciences
T1  - Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia
VL  - 27
IS  - 1
SP  - 518
EP  - 523
DO  - 10.1016/j.sjbs.2019.11.012
ER  - 

@article{
author = "Matović, Kazimir and Vidanović, Dejan and Manić, Marija and Stojiljković, Marko and Radojičić, Sonja and Debeljak, Zoran and Šekler, Milanko and Ćirić, Jelena",
year = "2020",
abstract = "A total of 7386 samples of adult honey bees from different areas of Serbia (fifteen regions and 79 municipalities) were selected for light microscopy analysis for Nosema species during 1992-2017. A selection of honey bee samples from colonies positive for microsporidian spores during 2009-2011, 2015 and 2017 were then subjected to molecular diagnosis by multiplex PCR using specific primers for a region of the 16S rRNA gene of Nosema species. The prevalence of microsporidian spore-positive bee colonies ranged between 14.4% in 2013 and 65.4% in 1992. PCR results show that Nosema ceranae is not the only Nosema species to infect honey bees in Serbia. Mixed N. apis/N. ceranae infections were detected in the two honey bee samples examined by mPCR during 2017. The beekeeping management of disease prevention, such as replacement of combs and queens and hygienic handling of colonies are useful in the prevention of Nosema infection.",
publisher = "Elsevier, Amsterdam",
journal = "Saudi Journal of Biological Sciences",
title = "Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia",
volume = "27",
number = "1",
pages = "518-523",
doi = "10.1016/j.sjbs.2019.11.012"
}

Matović, K., Vidanović, D., Manić, M., Stojiljković, M., Radojičić, S., Debeljak, Z., Šekler, M.,& Ćirić, J.. (2020). Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia. in Saudi Journal of Biological Sciences
Elsevier, Amsterdam., 27(1), 518-523.
https://doi.org/10.1016/j.sjbs.2019.11.012

Matović K, Vidanović D, Manić M, Stojiljković M, Radojičić S, Debeljak Z, Šekler M, Ćirić J. Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia. in Saudi Journal of Biological Sciences. 2020;27(1):518-523.
doi:10.1016/j.sjbs.2019.11.012 .

Matović, Kazimir, Vidanović, Dejan, Manić, Marija, Stojiljković, Marko, Radojičić, Sonja, Debeljak, Zoran, Šekler, Milanko, Ćirić, Jelena, "Twenty-five-year study of Nosema spp. in honey bees (Apis mellifera) in Serbia" in Saudi Journal of Biological Sciences, 27, no. 1 (2020):518-523,
https://doi.org/10.1016/j.sjbs.2019.11.012 . .

TY  - JOUR
AU  - Milovanović, Milovan
AU  - Milićević, Vesna
AU  - Valčić, Miroslav
AU  - Stević, Nataša
AU  - Nišavić, Jakov
AU  - Radojičić, Sonja
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1781
AB  - Infection of sheep by the ORF virus (ORFV) is very common in Serbia. ORFV is an economically important viral disease, distributed worldwide. Phylogenetic analysis based on the B2L gene of Serbian ORFV strains from two outbreaks that occurred in Serbia in 2016 is presented in this paper. Crust formation around the lips, nostrils, and udder was noted in all animals from the first outbreak, whilst in the second outbreak, all animals showed swollen and cyanotic lips and muzzle, with no visible crusts. Virus isolation was conducted using Vero cells. Cytopathic effects were evident on the third passage. However, all examined samples were positive using PCR. Phylogenetic analysis of the partial gene sequences (terminal gene regions were not included) encoding B2L gene of Serbian ORFV isolates showed 97.33-100.00% nucleotide and 92.86-100.00% amino acid similarity between each other. However, the viruses were divided into two clusters within the previously recognized Group 2, together with viruses from Croatia, Greece, Finland, China, South Korea and North America. This study is the first report of phylogenetic analysis of ORFV from Serbia and contributes to the data available in the GenBank database. The results of our investigation showed genetic diversity between ORFV strains in Serbia.
PB  - Univ Agriculture, Fac Veterinary Science, Faisalabad
T2  - Pakistan Veterinary Journal
T1  - Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia
VL  - 39
IS  - 3
SP  - 433
EP  - 437
DO  - 10.29261/pakvetj/2019.034
ER  - 

@article{
author = "Milovanović, Milovan and Milićević, Vesna and Valčić, Miroslav and Stević, Nataša and Nišavić, Jakov and Radojičić, Sonja",
year = "2019",
abstract = "Infection of sheep by the ORF virus (ORFV) is very common in Serbia. ORFV is an economically important viral disease, distributed worldwide. Phylogenetic analysis based on the B2L gene of Serbian ORFV strains from two outbreaks that occurred in Serbia in 2016 is presented in this paper. Crust formation around the lips, nostrils, and udder was noted in all animals from the first outbreak, whilst in the second outbreak, all animals showed swollen and cyanotic lips and muzzle, with no visible crusts. Virus isolation was conducted using Vero cells. Cytopathic effects were evident on the third passage. However, all examined samples were positive using PCR. Phylogenetic analysis of the partial gene sequences (terminal gene regions were not included) encoding B2L gene of Serbian ORFV isolates showed 97.33-100.00% nucleotide and 92.86-100.00% amino acid similarity between each other. However, the viruses were divided into two clusters within the previously recognized Group 2, together with viruses from Croatia, Greece, Finland, China, South Korea and North America. This study is the first report of phylogenetic analysis of ORFV from Serbia and contributes to the data available in the GenBank database. The results of our investigation showed genetic diversity between ORFV strains in Serbia.",
publisher = "Univ Agriculture, Fac Veterinary Science, Faisalabad",
journal = "Pakistan Veterinary Journal",
title = "Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia",
volume = "39",
number = "3",
pages = "433-437",
doi = "10.29261/pakvetj/2019.034"
}

Milovanović, M., Milićević, V., Valčić, M., Stević, N., Nišavić, J.,& Radojičić, S.. (2019). Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia. in Pakistan Veterinary Journal
Univ Agriculture, Fac Veterinary Science, Faisalabad., 39(3), 433-437.
https://doi.org/10.29261/pakvetj/2019.034

Milovanović M, Milićević V, Valčić M, Stević N, Nišavić J, Radojičić S. Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia. in Pakistan Veterinary Journal. 2019;39(3):433-437.
doi:10.29261/pakvetj/2019.034 .

Milovanović, Milovan, Milićević, Vesna, Valčić, Miroslav, Stević, Nataša, Nišavić, Jakov, Radojičić, Sonja, "Detection and Phylogenetic Analysis of B2L Gene of ORF Virus from Clinical Cases of Sheep in Serbia" in Pakistan Veterinary Journal, 39, no. 3 (2019):433-437,
https://doi.org/10.29261/pakvetj/2019.034 . .

TY  - CONF
AU  - Valčić, Miroslav A.
AU  - Radojičić, Sonja
AU  - Stević, Nataša
AU  - Milovanoviić, Milovan
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3432
PB  - Požarevac : Veterinarski specijalistički institut
C3  - 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
T1  - Varijacije u kliničkoj slici afričke kuge svinja – iskustva i očekivanja u slučaju epizootije
SP  - 29
EP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3432
ER  - 

@conference{
author = "Valčić, Miroslav A. and Radojičić, Sonja and Stević, Nataša and Milovanoviić, Milovan",
year = "2019",
publisher = "Požarevac : Veterinarski specijalistički institut",
journal = "17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019",
title = "Varijacije u kliničkoj slici afričke kuge svinja – iskustva i očekivanja u slučaju epizootije",
pages = "29-38",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3432"
}

Valčić, M. A., Radojičić, S., Stević, N.,& Milovanoviić, M.. (2019). Varijacije u kliničkoj slici afričke kuge svinja – iskustva i očekivanja u slučaju epizootije. in 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
Požarevac : Veterinarski specijalistički institut., 29-38.
https://hdl.handle.net/21.15107/rcub_veterinar_3432

Valčić MA, Radojičić S, Stević N, Milovanoviić M. Varijacije u kliničkoj slici afričke kuge svinja – iskustva i očekivanja u slučaju epizootije. in 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019. 2019;:29-38.
https://hdl.handle.net/21.15107/rcub_veterinar_3432 .

Valčić, Miroslav A., Radojičić, Sonja, Stević, Nataša, Milovanoviić, Milovan, "Varijacije u kliničkoj slici afričke kuge svinja – iskustva i očekivanja u slučaju epizootije" in 17. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019 (2019):29-38,
https://hdl.handle.net/21.15107/rcub_veterinar_3432 .

TY  - JOUR
AU  - Živojinović, Milena
AU  - Dobrosavljević, Ivan
AU  - Kulišić, Zoran
AU  - Radojičić, Sonja
AU  - Bošković, Tamara
AU  - Plavšić, Budimir
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1707
AB  - Background. Trichinellosis is zoonotic disease caused by parasites of the genus Trichinella, which have a cosmopolitan distribution. In the Balkan region, including Serbia, trichinellosis is endemic. Although trichinellosis shows a decreasing incidence in Serbia, this disease remains a serious problem for human health and animal husbandry. Scope and Approach. To prevent and control Trichinella spp. infections in domestic and sylvatic animals, risk analysis for these zoonotic parasites should be conducted. In Serbia, a limited number of Trichinella have been identified to the species level so far. An institutional, multi-sectoral surveillance system, supported by an adequate legal framework for the detection, surveillance, prevention, control and reporting of this infection in animals and humans, harmonized with the EU legislation, is a priority. Key Findings and Conclusions. One of the main goals of the Serbian veterinary and public health services should be progressive improvement of animal rearing practices, food safety, and hunters' and consumers' education through the "One-health" approach.
AB  - Uvod. Trihineloza je zoonotska bolest uzrokovana parazitima roda Trichinella koji imaju kosmopolitsku rasprostranjenost. U regionu Balkana, uključujući Srbiju, trihineloza je endemska. Iako trihineloza pokazuje trend smanjenja učestalosti u Srbiji, ova bolest ostaje ozbiljan problem za zdravlje ljudi i stočarstvo. Cilj i pristup. Za sprečavanje i kontrolu infekcije domaćih i silvatičnih životinja trihinelama, treba sprovesti analizu rizika za ove zoonotske parazite. U Srbiji je do sada identifikovan ograničeni broj trihinela na nivou vrste. U tom smislu, priorit je institucionalni, multisektorski sistem nadzora, podržan odgovarajućim pravnim okvirom za otkrivanje, nadzor, prevenciju, kontrolu i izveštavanje o ovoj infekciji kod životinja i ljudi, usklađen sa zakonodavstvom EU. Ključni nalazi i zaključak. Jedan od glavnih ciljeva veterinarske i zdravstvene službe trebalo bi biti progresivno unapređenje prakse uzgoja životinja, bezbednost hrane i obrazovanja lovaca i potrošača kroz pristup "One-Health".
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Trichinellosis in Serbia and possibilities to improve control measures
T1  - Trihineloza u Srbiji i mogućnost unapređenja kontrolnih mera
VL  - 73
IS  - 2
SP  - 108
EP  - 115
DO  - 10.2298/VETGL190412018Z
ER  - 

@article{
author = "Živojinović, Milena and Dobrosavljević, Ivan and Kulišić, Zoran and Radojičić, Sonja and Bošković, Tamara and Plavšić, Budimir",
year = "2019",
abstract = "Background. Trichinellosis is zoonotic disease caused by parasites of the genus Trichinella, which have a cosmopolitan distribution. In the Balkan region, including Serbia, trichinellosis is endemic. Although trichinellosis shows a decreasing incidence in Serbia, this disease remains a serious problem for human health and animal husbandry. Scope and Approach. To prevent and control Trichinella spp. infections in domestic and sylvatic animals, risk analysis for these zoonotic parasites should be conducted. In Serbia, a limited number of Trichinella have been identified to the species level so far. An institutional, multi-sectoral surveillance system, supported by an adequate legal framework for the detection, surveillance, prevention, control and reporting of this infection in animals and humans, harmonized with the EU legislation, is a priority. Key Findings and Conclusions. One of the main goals of the Serbian veterinary and public health services should be progressive improvement of animal rearing practices, food safety, and hunters' and consumers' education through the "One-health" approach., Uvod. Trihineloza je zoonotska bolest uzrokovana parazitima roda Trichinella koji imaju kosmopolitsku rasprostranjenost. U regionu Balkana, uključujući Srbiju, trihineloza je endemska. Iako trihineloza pokazuje trend smanjenja učestalosti u Srbiji, ova bolest ostaje ozbiljan problem za zdravlje ljudi i stočarstvo. Cilj i pristup. Za sprečavanje i kontrolu infekcije domaćih i silvatičnih životinja trihinelama, treba sprovesti analizu rizika za ove zoonotske parazite. U Srbiji je do sada identifikovan ograničeni broj trihinela na nivou vrste. U tom smislu, priorit je institucionalni, multisektorski sistem nadzora, podržan odgovarajućim pravnim okvirom za otkrivanje, nadzor, prevenciju, kontrolu i izveštavanje o ovoj infekciji kod životinja i ljudi, usklađen sa zakonodavstvom EU. Ključni nalazi i zaključak. Jedan od glavnih ciljeva veterinarske i zdravstvene službe trebalo bi biti progresivno unapređenje prakse uzgoja životinja, bezbednost hrane i obrazovanja lovaca i potrošača kroz pristup "One-Health".",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Trichinellosis in Serbia and possibilities to improve control measures, Trihineloza u Srbiji i mogućnost unapređenja kontrolnih mera",
volume = "73",
number = "2",
pages = "108-115",
doi = "10.2298/VETGL190412018Z"
}

Živojinović, M., Dobrosavljević, I., Kulišić, Z., Radojičić, S., Bošković, T.,& Plavšić, B.. (2019). Trichinellosis in Serbia and possibilities to improve control measures. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 73(2), 108-115.
https://doi.org/10.2298/VETGL190412018Z

Živojinović M, Dobrosavljević I, Kulišić Z, Radojičić S, Bošković T, Plavšić B. Trichinellosis in Serbia and possibilities to improve control measures. in Veterinarski Glasnik. 2019;73(2):108-115.
doi:10.2298/VETGL190412018Z .

Živojinović, Milena, Dobrosavljević, Ivan, Kulišić, Zoran, Radojičić, Sonja, Bošković, Tamara, Plavšić, Budimir, "Trichinellosis in Serbia and possibilities to improve control measures" in Veterinarski Glasnik, 73, no. 2 (2019):108-115,
https://doi.org/10.2298/VETGL190412018Z . .

TY  - JOUR
AU  - Milovanović, Milovan
AU  - Dietze, Klaas
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Moritz, Tom
AU  - Hoffmann, Bernd
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1732
AB  - BackgroundIn the presented study we investigated the development of the humoral immune response against LSDV during the process of re-vaccination of cattle over a time span of 5 months. In addition, the performance of different serological techniques for antibody detection against LSDV was compared. For sample collection, an area without previous LSD outbreak reports in Serbia was selected. Seventy-nine cattle from twenty farms vaccinated in 2016 and re-vaccinated in 2017 were included in the study. Two farms from the same area with good calving management were selected for investigation of passive LSDV antibody transfer from vaccinated mothers to new-borne calves.ResultsAll investigated cattle were healthy on the day of vaccination and during the whole study. Swelling at the injection site or other side effects of vaccination did not occur after re-vaccination in the study.Detection of LSD-specific antibodies was performed with the standard serological methods VNT and IFAT as well as a commercially available Capripox double antigen multi-species-ELISA. Capripoxvirus-specific antibodies were detected 46 to 47weeks after vaccination in 2016, with VNT in 35.06% and with IFAT and ELISA in 33.77%. A secondary response was observed in all three tests 1 month after re-vaccination with a significant increase in seropositive animals compared to the results before re-vaccination. With all applied serological methods, the number of animals testing positive was significantly higher at 1 and 5 months post re-vaccination than before re-vaccination. No significant statistical difference (p>0.05) was observed between the results of all three tests used. The sensitivity and specificity of ELISA was estimated to be Se-ELISA 91% and Sp(ELISA) 87% calculated by the results of VNT and Se-ELISA 88% and Sp(ELISA) 76% calculated by the results of IFAT. Passive antibody transfer from vaccinated mothers to new-born calves was investigated at 14days after birth. Discrepancies for the detection of LSDV specific antibodies between cows and newborn calves at the age of 14days were observed in VNT and IFAT, but not in ELISA.ConclusionOf all tests used the commercially available ELISA shows to be the most useful for high throughput analysis compared to VNT or IFAT.
PB  - BMC, London
T2  - BMC Veterinary Research
T1  - Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests
VL  - 15
SP  - 80
DO  - 10.1186/s12917-019-1831-y
ER  - 

@article{
author = "Milovanović, Milovan and Dietze, Klaas and Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Moritz, Tom and Hoffmann, Bernd",
year = "2019",
abstract = "BackgroundIn the presented study we investigated the development of the humoral immune response against LSDV during the process of re-vaccination of cattle over a time span of 5 months. In addition, the performance of different serological techniques for antibody detection against LSDV was compared. For sample collection, an area without previous LSD outbreak reports in Serbia was selected. Seventy-nine cattle from twenty farms vaccinated in 2016 and re-vaccinated in 2017 were included in the study. Two farms from the same area with good calving management were selected for investigation of passive LSDV antibody transfer from vaccinated mothers to new-borne calves.ResultsAll investigated cattle were healthy on the day of vaccination and during the whole study. Swelling at the injection site or other side effects of vaccination did not occur after re-vaccination in the study.Detection of LSD-specific antibodies was performed with the standard serological methods VNT and IFAT as well as a commercially available Capripox double antigen multi-species-ELISA. Capripoxvirus-specific antibodies were detected 46 to 47weeks after vaccination in 2016, with VNT in 35.06% and with IFAT and ELISA in 33.77%. A secondary response was observed in all three tests 1 month after re-vaccination with a significant increase in seropositive animals compared to the results before re-vaccination. With all applied serological methods, the number of animals testing positive was significantly higher at 1 and 5 months post re-vaccination than before re-vaccination. No significant statistical difference (p>0.05) was observed between the results of all three tests used. The sensitivity and specificity of ELISA was estimated to be Se-ELISA 91% and Sp(ELISA) 87% calculated by the results of VNT and Se-ELISA 88% and Sp(ELISA) 76% calculated by the results of IFAT. Passive antibody transfer from vaccinated mothers to new-born calves was investigated at 14days after birth. Discrepancies for the detection of LSDV specific antibodies between cows and newborn calves at the age of 14days were observed in VNT and IFAT, but not in ELISA.ConclusionOf all tests used the commercially available ELISA shows to be the most useful for high throughput analysis compared to VNT or IFAT.",
publisher = "BMC, London",
journal = "BMC Veterinary Research",
title = "Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests",
volume = "15",
pages = "80",
doi = "10.1186/s12917-019-1831-y"
}

Milovanović, M., Dietze, K., Milićević, V., Radojičić, S., Valčić, M., Moritz, T.,& Hoffmann, B.. (2019). Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests. in BMC Veterinary Research
BMC, London., 15, 80.
https://doi.org/10.1186/s12917-019-1831-y

Milovanović M, Dietze K, Milićević V, Radojičić S, Valčić M, Moritz T, Hoffmann B. Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests. in BMC Veterinary Research. 2019;15:80.
doi:10.1186/s12917-019-1831-y .

Milovanović, Milovan, Dietze, Klaas, Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Moritz, Tom, Hoffmann, Bernd, "Humoral immune response to repeated lumpy skin disease virus vaccination and performance of serological tests" in BMC Veterinary Research, 15 (2019):80,
https://doi.org/10.1186/s12917-019-1831-y . .

TY  - JOUR
AU  - Sukara, Ratko
AU  - Juwaid, Salem
AU  - Ćirović, Duško
AU  - Penezić, Aleksandra
AU  - Mihaljica, Darko
AU  - Veinović, Gorana
AU  - Radojičić, Sonja
AU  - Hodzić, Adnan
AU  - Duscher, Gerhard Georg
AU  - Tomanović, Snežana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1767
AB  - Human activities such as deforestation, urbanization, and environmental pollution lead to a reduction in the spatial boundary between wild animals, domestic animals and humans. These activities increase the risk for the emergence of pathogens from the sylvatic cycle in the population of domestic animals and humans. Foxes are recognized as potential reservoirs for a number of bacterial pathogens of medical and public health concern. The aim of the present study was to investigate the prevalence and spatial distribution of bacterial tick-borne pathogens from the Anaplasmataceae family, Borrelia burgdorferi sensu lato (s.l.), Rickettsia spp., Coxiella burnetii, Francisella tularensis, Bartonella spp., in the red fox population from Serbia and to discuss the obtained results from the epidemiological point of view. Legally hunted red foxes (Vulpes vulpes) from 14 localities in Serbia were included in the study and spleen samples from 129 animals were tested with conventional PCR assays for the presence of bacterial tick-borne pathogens. DNA of Candidatus Neoehrlichia sp. (FU98), Borrelia burgdorferi sensu stricto, Borrelia lusitaniae, and Borrelia garinii was detected in 6 (4.7%), 1 (0.8%), 2 (1.6%) and 1 (0.8%) animals, respectively. Co-infection by Candidatus Neoehrlichia sp. (FU98) and B. garinii was detected in one animal. All samples were negative for other tested bacterial tick-borne pathogens. The results of the present study indicate the potential role of foxes in natural cycles of Candidatus Neoehrlichia sp. (FU98) and causative agents of Lyme borreliosis in the investigated areas. Further research is required to elucidate the role of foxes in the epidemiology of these and other tick-borne zoonotic pathogens in the Republic of Serbia.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia
VL  - 69
IS  - 3
SP  - 312
EP  - 324
DO  - 10.2478/acve-2019-0026
ER  - 

@article{
author = "Sukara, Ratko and Juwaid, Salem and Ćirović, Duško and Penezić, Aleksandra and Mihaljica, Darko and Veinović, Gorana and Radojičić, Sonja and Hodzić, Adnan and Duscher, Gerhard Georg and Tomanović, Snežana",
year = "2019",
abstract = "Human activities such as deforestation, urbanization, and environmental pollution lead to a reduction in the spatial boundary between wild animals, domestic animals and humans. These activities increase the risk for the emergence of pathogens from the sylvatic cycle in the population of domestic animals and humans. Foxes are recognized as potential reservoirs for a number of bacterial pathogens of medical and public health concern. The aim of the present study was to investigate the prevalence and spatial distribution of bacterial tick-borne pathogens from the Anaplasmataceae family, Borrelia burgdorferi sensu lato (s.l.), Rickettsia spp., Coxiella burnetii, Francisella tularensis, Bartonella spp., in the red fox population from Serbia and to discuss the obtained results from the epidemiological point of view. Legally hunted red foxes (Vulpes vulpes) from 14 localities in Serbia were included in the study and spleen samples from 129 animals were tested with conventional PCR assays for the presence of bacterial tick-borne pathogens. DNA of Candidatus Neoehrlichia sp. (FU98), Borrelia burgdorferi sensu stricto, Borrelia lusitaniae, and Borrelia garinii was detected in 6 (4.7%), 1 (0.8%), 2 (1.6%) and 1 (0.8%) animals, respectively. Co-infection by Candidatus Neoehrlichia sp. (FU98) and B. garinii was detected in one animal. All samples were negative for other tested bacterial tick-borne pathogens. The results of the present study indicate the potential role of foxes in natural cycles of Candidatus Neoehrlichia sp. (FU98) and causative agents of Lyme borreliosis in the investigated areas. Further research is required to elucidate the role of foxes in the epidemiology of these and other tick-borne zoonotic pathogens in the Republic of Serbia.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia",
volume = "69",
number = "3",
pages = "312-324",
doi = "10.2478/acve-2019-0026"
}

Sukara, R., Juwaid, S., Ćirović, D., Penezić, A., Mihaljica, D., Veinović, G., Radojičić, S., Hodzić, A., Duscher, G. G.,& Tomanović, S.. (2019). Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3), 312-324.
https://doi.org/10.2478/acve-2019-0026

Sukara R, Juwaid S, Ćirović D, Penezić A, Mihaljica D, Veinović G, Radojičić S, Hodzić A, Duscher GG, Tomanović S. Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia. in Acta Veterinaria-Beograd. 2019;69(3):312-324.
doi:10.2478/acve-2019-0026 .

Sukara, Ratko, Juwaid, Salem, Ćirović, Duško, Penezić, Aleksandra, Mihaljica, Darko, Veinović, Gorana, Radojičić, Sonja, Hodzić, Adnan, Duscher, Gerhard Georg, Tomanović, Snežana, "Candidatus neoehrlichia sp. (fu98) and borrelia burgdorferi sensu lato in red foxes (vulpes vulpes) from Serbia" in Acta Veterinaria-Beograd, 69, no. 3 (2019):312-324,
https://doi.org/10.2478/acve-2019-0026 . .

TY  - CONF
AU  - Milovanović, Milovan
AU  - Milićević, Vesna
AU  - Radojičić, Sonja
AU  - Valčić, Miroslav
AU  - Dietze, Klaas
AU  - Hoffmann, Bernd
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2753
AB  - The commercially available ID Screen® Capripox double antigen ELISA from IDvet® validated and
authorized for serum and plasma, recently improved the options for assessing the humoral immune
response against lumpy skin disease (LSD). In this study, the suitability of milk (individual and bulk)
and colostrum as samples for this ELISA was investigated.
Samples (serum, milk and colostrum) were collected three times (before, one and five months post
re-vaccination) in LSDV-field strain free regions in Serbia. Test specificity was confirmed by
analyzing of 352 individual milk samples from Germany.
From 154 collected serum samples from Serbia, 75 were detected as positive by the Capripox
ELISA. Milk samples were tested using protocol for serum and with a modified protocol (increased
incubation time). Obtained results of milk and colostrum samples were analyzed with reduced cutoff value. Out of 154 milk samples, 38 samples were detected as positive using the protocol for
serum, whereas with modified protocol the number of positive sample increased up to 48. Only
two milk samples from Germany had border line results with the modified protocol. Sensitivity and
specificity of the ELISA for milk samples reached values in-between 88% to 91%, with significant
statistical difference (p<0.05) between both protocols. In addition, antibody detection was
possible in bulk milk and colostrum samples.
In principle, the commercially available Capripox ELISA, developed for antibody detection in serum
and plasma, shows to be suitable for detection of LSD-specific antibodies in milk samples and can
be a helpful tool in LSDV monitoring programs.
C3  - 13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019
T1  - Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination
SP  - 204
EP  - 204
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2753
ER  - 

@conference{
author = "Milovanović, Milovan and Milićević, Vesna and Radojičić, Sonja and Valčić, Miroslav and Dietze, Klaas and Hoffmann, Bernd",
year = "2019",
abstract = "The commercially available ID Screen® Capripox double antigen ELISA from IDvet® validated and
authorized for serum and plasma, recently improved the options for assessing the humoral immune
response against lumpy skin disease (LSD). In this study, the suitability of milk (individual and bulk)
and colostrum as samples for this ELISA was investigated.
Samples (serum, milk and colostrum) were collected three times (before, one and five months post
re-vaccination) in LSDV-field strain free regions in Serbia. Test specificity was confirmed by
analyzing of 352 individual milk samples from Germany.
From 154 collected serum samples from Serbia, 75 were detected as positive by the Capripox
ELISA. Milk samples were tested using protocol for serum and with a modified protocol (increased
incubation time). Obtained results of milk and colostrum samples were analyzed with reduced cutoff value. Out of 154 milk samples, 38 samples were detected as positive using the protocol for
serum, whereas with modified protocol the number of positive sample increased up to 48. Only
two milk samples from Germany had border line results with the modified protocol. Sensitivity and
specificity of the ELISA for milk samples reached values in-between 88% to 91%, with significant
statistical difference (p<0.05) between both protocols. In addition, antibody detection was
possible in bulk milk and colostrum samples.
In principle, the commercially available Capripox ELISA, developed for antibody detection in serum
and plasma, shows to be suitable for detection of LSD-specific antibodies in milk samples and can
be a helpful tool in LSDV monitoring programs.",
journal = "13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019",
title = "Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination",
pages = "204-204",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2753"
}

Milovanović, M., Milićević, V., Radojičić, S., Valčić, M., Dietze, K.,& Hoffmann, B.. (2019). Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination. in 13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019, 204-204.
https://hdl.handle.net/21.15107/rcub_veterinar_2753

Milovanović M, Milićević V, Radojičić S, Valčić M, Dietze K, Hoffmann B. Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination. in 13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019. 2019;:204-204.
https://hdl.handle.net/21.15107/rcub_veterinar_2753 .

Milovanović, Milovan, Milićević, Vesna, Radojičić, Sonja, Valčić, Miroslav, Dietze, Klaas, Hoffmann, Bernd, "Suitability of individual and bulk milk samples to investigate the humoral immune responses to LSD vaccination" in 13th Epizoone Annual Meeting ‚‚Breaking Walls" Berlin, Germany, 26-28 August 2019 (2019):204-204,
https://hdl.handle.net/21.15107/rcub_veterinar_2753 .

TY  - JOUR
AU  - Bogunović, Danica
AU  - Stević, Nataša
AU  - Sidi-Boumedine, Karim
AU  - Mišić, Dušan
AU  - Tomanović, Snežana
AU  - Kulišić, Zoran
AU  - Magaš, Vladimir
AU  - Radojičić, Sonja
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1589
AB  - Q fever is a zoonotic disease caused by Coxiella burnetii, a gram-negative coccobacillus, which has been detected in a wide range of animal species, mostly domestic ruminants, but also in wild mammals, pets, birds, reptiles, arthropods (especially ticks), as well as in humans. Although the exposure to domestic animals in rural areas is regarded as the most common cause of the disease in humans, recent studies have shown that the role of pets in the epidemiology of Q fever has been increasingly growing. Although the primary route of infection is inhalation, it is presumed that among animals the infection circulates through ticks and that they are responsible for heterospecific transmission, as well as spatial dispersion among vertebrates. The aim of this study was to determine the presence and prevalence of C. burnetii in ticks removed from stray dogs, as well as to examine the distribution of tick species parasitizing dogs on the territory of Belgrade city. A PCR protocol targeting IS1111 repetitive transposon-like region of C. burnetii was used for the detection of C. burnetii DNA in ticks and the results were confirmed by sequence analysis. In total, 316 ticks were collected from 51 stray dogs - 40 females (78.43%) and 11 males (21.57%). Three species of ticks were identified: Rhipicephalus sanguineus (72.15%), Ixodes ricinus (27.53%) and Dermacentor reticulatus (0.32%). Out of 316 examined ticks, C. burnetii DNA was detected only in the brown dog tick R. sanguineus, with a total prevalence of 10.53% (24/228). The high prevalence of C. burnetii in R. sanguineus, which is primarily a dog tick, indicates the importance of dogs in the epidemiology of Q fever in the territory of Belgrade.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia)
VL  - 68
IS  - 3
SP  - 257
EP  - 268
DO  - 10.2478/acve-2018-0023
ER  - 

@article{
author = "Bogunović, Danica and Stević, Nataša and Sidi-Boumedine, Karim and Mišić, Dušan and Tomanović, Snežana and Kulišić, Zoran and Magaš, Vladimir and Radojičić, Sonja",
year = "2018",
abstract = "Q fever is a zoonotic disease caused by Coxiella burnetii, a gram-negative coccobacillus, which has been detected in a wide range of animal species, mostly domestic ruminants, but also in wild mammals, pets, birds, reptiles, arthropods (especially ticks), as well as in humans. Although the exposure to domestic animals in rural areas is regarded as the most common cause of the disease in humans, recent studies have shown that the role of pets in the epidemiology of Q fever has been increasingly growing. Although the primary route of infection is inhalation, it is presumed that among animals the infection circulates through ticks and that they are responsible for heterospecific transmission, as well as spatial dispersion among vertebrates. The aim of this study was to determine the presence and prevalence of C. burnetii in ticks removed from stray dogs, as well as to examine the distribution of tick species parasitizing dogs on the territory of Belgrade city. A PCR protocol targeting IS1111 repetitive transposon-like region of C. burnetii was used for the detection of C. burnetii DNA in ticks and the results were confirmed by sequence analysis. In total, 316 ticks were collected from 51 stray dogs - 40 females (78.43%) and 11 males (21.57%). Three species of ticks were identified: Rhipicephalus sanguineus (72.15%), Ixodes ricinus (27.53%) and Dermacentor reticulatus (0.32%). Out of 316 examined ticks, C. burnetii DNA was detected only in the brown dog tick R. sanguineus, with a total prevalence of 10.53% (24/228). The high prevalence of C. burnetii in R. sanguineus, which is primarily a dog tick, indicates the importance of dogs in the epidemiology of Q fever in the territory of Belgrade.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia)",
volume = "68",
number = "3",
pages = "257-268",
doi = "10.2478/acve-2018-0023"
}

Bogunović, D., Stević, N., Sidi-Boumedine, K., Mišić, D., Tomanović, S., Kulišić, Z., Magaš, V.,& Radojičić, S.. (2018). Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia). in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(3), 257-268.
https://doi.org/10.2478/acve-2018-0023

Bogunović D, Stević N, Sidi-Boumedine K, Mišić D, Tomanović S, Kulišić Z, Magaš V, Radojičić S. Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia). in Acta Veterinaria-Beograd. 2018;68(3):257-268.
doi:10.2478/acve-2018-0023 .

Bogunović, Danica, Stević, Nataša, Sidi-Boumedine, Karim, Mišić, Dušan, Tomanović, Snežana, Kulišić, Zoran, Magaš, Vladimir, Radojičić, Sonja, "Molecular evidence of q fever agent coxiella burnetii in ixodid ticks collected from stray dogs in Belgrade (Serbia)" in Acta Veterinaria-Beograd, 68, no. 3 (2018):257-268,
https://doi.org/10.2478/acve-2018-0023 . .

TY  - JOUR
AU  - Gavrilović, A.
AU  - Gavrilović, P.
AU  - Radojičić, Sonja
AU  - Krnjaić, Dejan
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1570
AB  - Bluetongue (BT) is a viral non-contagious disease of ruminants which is transmitted by insects of the genus Culicoides. In recent years, BT has been a serious threat to livestock and to the economies of European countries. In Serbia the disease appeared for the first time in 2001, and after a 12 year period of freedom, it broke out again in 2014. Considering the actuality of this infectious disease, especially the need for prompt and rapid diagnostics, the aim of this paper was to determine the possibility of detecting the serological response in sheep and cattle with manifested clinical signs of the disease using two different methods: double recognition enzyme-linked immunosorbent assay (sELISA) and competitive enzyme-linked immunosorbent assay (cELISA). A total of 105 blood serum samples of cattle and sheep, which had exhibited clinical signs of BT during 2014, were taken for examination from a serum bank. Out of 74 blood serum samples of sheep and 31 blood serum samples of cattle, 52 samples of sheep and 18 samples of cattle tested positive using sELISA, while 50 samples of sheep and 18 samples of cattle gave positive reactions with cELISA. The results confirm the high sensitivity of sELISA which detected 4% more seropositive sheep in comparison with cELISA. Using Cohens kappa statistical analysis, almost perfect agreement was determined between the results (k>0,81) obtained by cELISA and sELISA.
PB  - Hellenic Veterinary Medical Soc, Athens
T2  - Journal of The Hellenic Veterinary Medical Society
T1  - Comparative examination of the serological response to bluetongue virus in diseased ruminants by competitive and double recognition enzime-linked immunosorbent assays
VL  - 69
IS  - 3
SP  - 1088
EP  - 1093
DO  - 10.12681/jhvms.18880
ER  - 

@article{
author = "Gavrilović, A. and Gavrilović, P. and Radojičić, Sonja and Krnjaić, Dejan",
year = "2018",
abstract = "Bluetongue (BT) is a viral non-contagious disease of ruminants which is transmitted by insects of the genus Culicoides. In recent years, BT has been a serious threat to livestock and to the economies of European countries. In Serbia the disease appeared for the first time in 2001, and after a 12 year period of freedom, it broke out again in 2014. Considering the actuality of this infectious disease, especially the need for prompt and rapid diagnostics, the aim of this paper was to determine the possibility of detecting the serological response in sheep and cattle with manifested clinical signs of the disease using two different methods: double recognition enzyme-linked immunosorbent assay (sELISA) and competitive enzyme-linked immunosorbent assay (cELISA). A total of 105 blood serum samples of cattle and sheep, which had exhibited clinical signs of BT during 2014, were taken for examination from a serum bank. Out of 74 blood serum samples of sheep and 31 blood serum samples of cattle, 52 samples of sheep and 18 samples of cattle tested positive using sELISA, while 50 samples of sheep and 18 samples of cattle gave positive reactions with cELISA. The results confirm the high sensitivity of sELISA which detected 4% more seropositive sheep in comparison with cELISA. Using Cohens kappa statistical analysis, almost perfect agreement was determined between the results (k>0,81) obtained by cELISA and sELISA.",
publisher = "Hellenic Veterinary Medical Soc, Athens",
journal = "Journal of The Hellenic Veterinary Medical Society",
title = "Comparative examination of the serological response to bluetongue virus in diseased ruminants by competitive and double recognition enzime-linked immunosorbent assays",
volume = "69",
number = "3",
pages = "1088-1093",
doi = "10.12681/jhvms.18880"
}

Gavrilović, A., Gavrilović, P., Radojičić, S.,& Krnjaić, D.. (2018). Comparative examination of the serological response to bluetongue virus in diseased ruminants by competitive and double recognition enzime-linked immunosorbent assays. in Journal of The Hellenic Veterinary Medical Society
Hellenic Veterinary Medical Soc, Athens., 69(3), 1088-1093.
https://doi.org/10.12681/jhvms.18880

Gavrilović A, Gavrilović P, Radojičić S, Krnjaić D. Comparative examination of the serological response to bluetongue virus in diseased ruminants by competitive and double recognition enzime-linked immunosorbent assays. in Journal of The Hellenic Veterinary Medical Society. 2018;69(3):1088-1093.
doi:10.12681/jhvms.18880 .

Gavrilović, A., Gavrilović, P., Radojičić, Sonja, Krnjaić, Dejan, "Comparative examination of the serological response to bluetongue virus in diseased ruminants by competitive and double recognition enzime-linked immunosorbent assays" in Journal of The Hellenic Veterinary Medical Society, 69, no. 3 (2018):1088-1093,
https://doi.org/10.12681/jhvms.18880 . .

TY  - JOUR
AU  - Aničić, Milan
AU  - Vučićević, Ivana
AU  - Vasković, Nikola
AU  - Radojičić, Sonja
AU  - Juntes, Polona
AU  - Nešić, Slađan
AU  - Aleksić-Kovačević, Sanja
AU  - Marinković, Darko
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1616
AB  - Canine distemper virus (CDV) is a worldwide distributed RNA virus that can cause severe disease in carnivore and non-carnivore species. Red foxes are highly susceptible and may act as a reservoir of the virus. As in other wild species, distemper in red foxes can manifest as acute, systemic and chronic nervous form. In the present study, we detected antibodies against CDV among red foxes in Western Serbia, and analyzed histopathologically and immunohistochemically for CDV nuclear protein antigen (CDV-NP) brain samples derived from seropositive animals. Seroprevalence of CDV antibodies was 36.8%. Histopathological changes included gliosis, neuronal degeneration, satellitosis, mononuclear inflammation, demyelination and presence of inclusion bodies. Immunostaining showed a diffuse presence of CDV-NP antigen, mainly in the cytoplasm of astrocytes and neurons. Results of this work contribute to the opinion that red foxes act as a potential reservoir of CDV and underline the importance of routine vaccination of dogs that could come in close contact with these animals. Potential active surveillance program would give a better insight in the degree of CDV infection in wildlife.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Histopathological characteristics and expression of cdv-np antigen in the brain of serologically positive spontaneously infected red foxes (vulpes vulpes) in western Serbia
VL  - 68
IS  - 4
SP  - 434
EP  - 444
DO  - 10.2478/acve-2018-0035
ER  - 

@article{
author = "Aničić, Milan and Vučićević, Ivana and Vasković, Nikola and Radojičić, Sonja and Juntes, Polona and Nešić, Slađan and Aleksić-Kovačević, Sanja and Marinković, Darko",
year = "2018",
abstract = "Canine distemper virus (CDV) is a worldwide distributed RNA virus that can cause severe disease in carnivore and non-carnivore species. Red foxes are highly susceptible and may act as a reservoir of the virus. As in other wild species, distemper in red foxes can manifest as acute, systemic and chronic nervous form. In the present study, we detected antibodies against CDV among red foxes in Western Serbia, and analyzed histopathologically and immunohistochemically for CDV nuclear protein antigen (CDV-NP) brain samples derived from seropositive animals. Seroprevalence of CDV antibodies was 36.8%. Histopathological changes included gliosis, neuronal degeneration, satellitosis, mononuclear inflammation, demyelination and presence of inclusion bodies. Immunostaining showed a diffuse presence of CDV-NP antigen, mainly in the cytoplasm of astrocytes and neurons. Results of this work contribute to the opinion that red foxes act as a potential reservoir of CDV and underline the importance of routine vaccination of dogs that could come in close contact with these animals. Potential active surveillance program would give a better insight in the degree of CDV infection in wildlife.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Histopathological characteristics and expression of cdv-np antigen in the brain of serologically positive spontaneously infected red foxes (vulpes vulpes) in western Serbia",
volume = "68",
number = "4",
pages = "434-444",
doi = "10.2478/acve-2018-0035"
}

Aničić, M., Vučićević, I., Vasković, N., Radojičić, S., Juntes, P., Nešić, S., Aleksić-Kovačević, S.,& Marinković, D.. (2018). Histopathological characteristics and expression of cdv-np antigen in the brain of serologically positive spontaneously infected red foxes (vulpes vulpes) in western Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(4), 434-444.
https://doi.org/10.2478/acve-2018-0035

Aničić M, Vučićević I, Vasković N, Radojičić S, Juntes P, Nešić S, Aleksić-Kovačević S, Marinković D. Histopathological characteristics and expression of cdv-np antigen in the brain of serologically positive spontaneously infected red foxes (vulpes vulpes) in western Serbia. in Acta Veterinaria-Beograd. 2018;68(4):434-444.
doi:10.2478/acve-2018-0035 .

Aničić, Milan, Vučićević, Ivana, Vasković, Nikola, Radojičić, Sonja, Juntes, Polona, Nešić, Slađan, Aleksić-Kovačević, Sanja, Marinković, Darko, "Histopathological characteristics and expression of cdv-np antigen in the brain of serologically positive spontaneously infected red foxes (vulpes vulpes) in western Serbia" in Acta Veterinaria-Beograd, 68, no. 4 (2018):434-444,
https://doi.org/10.2478/acve-2018-0035 . .