TY  - JOUR
AU  - Gajić, Bojan
AU  - Stevanović, Jevrosima
AU  - Radulović, Željko
AU  - Kulišić, Zoran
AU  - Vejnović, Branislav
AU  - Glavinić, Uroš
AU  - Stanimirović, Zoran
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1422
AB  - In the present study, amplification refractory mutation system (ARMS) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were used for identification of recently described Serbia 1 (S1) and Peshter 1 (P1) mitochondrial haplotypes of Varroa destructor. Based on single nucleotide polymorphisms (SNPs) within cytochrome oxidase 1 (cox1) and cytochrome b (cytb) gene sequences, a total of 64 adult V. destructor females were analyzed from locations where the S1 and P1 haplotypes had been detected previously. Results of haplotype identification obtained by ARMS and PCR-RFLP methods were completely consistent with the sequencing data. Furthermore, in some analyzed samples the occurrence of site heteroplasmy at haplotype-defining sites was detected, as it was confirmed by double peaks in the sequence chromatograms. Neither mites with simultaneous nucleotide variability, nor those with combined SNP and heteroplasmy in cox1 and cytb were found. Given that this is the first occurrence of site heteroplasmy in V. destructor, the origin of this phenomenon and possible specific traits of heteroplasmic mites have yet to be determined.
PB  - Springer, Dordrecht
T2  - Experimental and Applied Acarology
T1  - Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods
VL  - 70
IS  - 3
SP  - 287
EP  - 297
DO  - 10.1007/s10493-016-0086-6
ER  - 

@article{
author = "Gajić, Bojan and Stevanović, Jevrosima and Radulović, Željko and Kulišić, Zoran and Vejnović, Branislav and Glavinić, Uroš and Stanimirović, Zoran",
year = "2016",
abstract = "In the present study, amplification refractory mutation system (ARMS) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods were used for identification of recently described Serbia 1 (S1) and Peshter 1 (P1) mitochondrial haplotypes of Varroa destructor. Based on single nucleotide polymorphisms (SNPs) within cytochrome oxidase 1 (cox1) and cytochrome b (cytb) gene sequences, a total of 64 adult V. destructor females were analyzed from locations where the S1 and P1 haplotypes had been detected previously. Results of haplotype identification obtained by ARMS and PCR-RFLP methods were completely consistent with the sequencing data. Furthermore, in some analyzed samples the occurrence of site heteroplasmy at haplotype-defining sites was detected, as it was confirmed by double peaks in the sequence chromatograms. Neither mites with simultaneous nucleotide variability, nor those with combined SNP and heteroplasmy in cox1 and cytb were found. Given that this is the first occurrence of site heteroplasmy in V. destructor, the origin of this phenomenon and possible specific traits of heteroplasmic mites have yet to be determined.",
publisher = "Springer, Dordrecht",
journal = "Experimental and Applied Acarology",
title = "Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods",
volume = "70",
number = "3",
pages = "287-297",
doi = "10.1007/s10493-016-0086-6"
}

Gajić, B., Stevanović, J., Radulović, Ž., Kulišić, Z., Vejnović, B., Glavinić, U.,& Stanimirović, Z.. (2016). Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods. in Experimental and Applied Acarology
Springer, Dordrecht., 70(3), 287-297.
https://doi.org/10.1007/s10493-016-0086-6

Gajić B, Stevanović J, Radulović Ž, Kulišić Z, Vejnović B, Glavinić U, Stanimirović Z. Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods. in Experimental and Applied Acarology. 2016;70(3):287-297.
doi:10.1007/s10493-016-0086-6 .

Gajić, Bojan, Stevanović, Jevrosima, Radulović, Željko, Kulišić, Zoran, Vejnović, Branislav, Glavinić, Uroš, Stanimirović, Zoran, "Haplotype identification and detection of mitochondrial DNA heteroplasmy in Varroa destructor mites using ARMS and PCR-RFLP methods" in Experimental and Applied Acarology, 70, no. 3 (2016):287-297,
https://doi.org/10.1007/s10493-016-0086-6 . .

TY  - JOUR
AU  - Obrenović, Sonja
AU  - Ristanović, Elizabeta
AU  - Cekanac, Radovan
AU  - Radulović, Željko
AU  - Ilić, Vesna
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1179
AB  - In this study the seroprevalence of anti-Borrelia burgdorferi IgG antibodies in populations of naturally exposed dogs in the Belgrade area was determined. Serum samples of 215 dogs were tested with in-house made indirect immunofluorescent-antibody assay (IFA), enzyme-linked immunosorbent assay (ELISA), and Western blot assay (WB). Sera were from 34 pet dogs suspected of having Lyme borreliosis, 41 hunting dogs, 125 shelter dogs, and 15 clinically healthy pet dogs. Results of WB were used as a standard against which the performances of ELISA and IFA were evaluated. ELISA was significantly more sensitive than IFA (84.8 vs 67.9%), whereas the specificities of these tests were just slightly different (94.4% vs 92.6 %). Borrelia burgdorferi seroprevalence of 24.7% among tested dogs was recorded by WB test. The highest seroprevalence of 31.2% was found in the group of shelter dogs, while hunting and pet dogs showed lower seroprevalence of 19.5% and 17.6%, but the difference was statistically insignificant. These results are the first report on the seroprevalence of IgG antibodies to B. burgdorferi in dogs in the Belgrade area and could be of importance in the assessment of the risk of infection with B. burgdorferi in human population.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Seroprevalence of igg antibodies against borrelia burgdorferi in dogs in Belgrade area, Serbia
VL  - 65
IS  - 1
SP  - 99
EP  - 110
DO  - 10.1515/acve-2015-0008
ER  - 

@article{
author = "Obrenović, Sonja and Ristanović, Elizabeta and Cekanac, Radovan and Radulović, Željko and Ilić, Vesna",
year = "2015",
abstract = "In this study the seroprevalence of anti-Borrelia burgdorferi IgG antibodies in populations of naturally exposed dogs in the Belgrade area was determined. Serum samples of 215 dogs were tested with in-house made indirect immunofluorescent-antibody assay (IFA), enzyme-linked immunosorbent assay (ELISA), and Western blot assay (WB). Sera were from 34 pet dogs suspected of having Lyme borreliosis, 41 hunting dogs, 125 shelter dogs, and 15 clinically healthy pet dogs. Results of WB were used as a standard against which the performances of ELISA and IFA were evaluated. ELISA was significantly more sensitive than IFA (84.8 vs 67.9%), whereas the specificities of these tests were just slightly different (94.4% vs 92.6 %). Borrelia burgdorferi seroprevalence of 24.7% among tested dogs was recorded by WB test. The highest seroprevalence of 31.2% was found in the group of shelter dogs, while hunting and pet dogs showed lower seroprevalence of 19.5% and 17.6%, but the difference was statistically insignificant. These results are the first report on the seroprevalence of IgG antibodies to B. burgdorferi in dogs in the Belgrade area and could be of importance in the assessment of the risk of infection with B. burgdorferi in human population.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Seroprevalence of igg antibodies against borrelia burgdorferi in dogs in Belgrade area, Serbia",
volume = "65",
number = "1",
pages = "99-110",
doi = "10.1515/acve-2015-0008"
}

Obrenović, S., Ristanović, E., Cekanac, R., Radulović, Ž.,& Ilić, V.. (2015). Seroprevalence of igg antibodies against borrelia burgdorferi in dogs in Belgrade area, Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 65(1), 99-110.
https://doi.org/10.1515/acve-2015-0008

Obrenović S, Ristanović E, Cekanac R, Radulović Ž, Ilić V. Seroprevalence of igg antibodies against borrelia burgdorferi in dogs in Belgrade area, Serbia. in Acta Veterinaria-Beograd. 2015;65(1):99-110.
doi:10.1515/acve-2015-0008 .

Obrenović, Sonja, Ristanović, Elizabeta, Cekanac, Radovan, Radulović, Željko, Ilić, Vesna, "Seroprevalence of igg antibodies against borrelia burgdorferi in dogs in Belgrade area, Serbia" in Acta Veterinaria-Beograd, 65, no. 1 (2015):99-110,
https://doi.org/10.1515/acve-2015-0008 . .

TY  - JOUR
AU  - Gajić, Bojan
AU  - Radulović, Željko
AU  - Stevanović, Jevrosima
AU  - Kulišić, Zoran
AU  - Vučićević, Miloš
AU  - Simeunović, Predrag
AU  - Stanimirović, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1050
AB  - Only two mitochondrial haplotypes (Korea and Japan) of Varroa destructor, the ectoparasitic honey bee mite, are known to be capable of infesting and successfully reproducing in Apis mellifera colonies worldwide. Varroa destructor (then called Varroa jacobsoni) was observed in Serbia for the first time in 1976. In order to obtain insight into the genetic variability of the mites parasitizing A. mellifera we analyzed 45 adult female mites sampled from nine localities dispersed throughout Serbia. Four fragments within cox1, atp6, cox3 and cytb mtDNA genes were sequenced. The Korea haplotype of V. destructor was found to be present at all localities, but also two new haplotypes (Serbia 1 and Peshter 1) were revealed, based on cox1 and cytb sequence variability. The simultaneous occurrence of Korea and Serbia 1 haplotypes was observed at five localities, whereas Peshter 1 haplotype was identifed at only one place.
PB  - Springer, Dordrecht
T2  - Experimental and Applied Acarology
T1  - Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis
VL  - 61
IS  - 1
SP  - 97
EP  - 105
DO  - 10.1007/s10493-013-9683-9
ER  - 

@article{
author = "Gajić, Bojan and Radulović, Željko and Stevanović, Jevrosima and Kulišić, Zoran and Vučićević, Miloš and Simeunović, Predrag and Stanimirović, Zoran",
year = "2013",
abstract = "Only two mitochondrial haplotypes (Korea and Japan) of Varroa destructor, the ectoparasitic honey bee mite, are known to be capable of infesting and successfully reproducing in Apis mellifera colonies worldwide. Varroa destructor (then called Varroa jacobsoni) was observed in Serbia for the first time in 1976. In order to obtain insight into the genetic variability of the mites parasitizing A. mellifera we analyzed 45 adult female mites sampled from nine localities dispersed throughout Serbia. Four fragments within cox1, atp6, cox3 and cytb mtDNA genes were sequenced. The Korea haplotype of V. destructor was found to be present at all localities, but also two new haplotypes (Serbia 1 and Peshter 1) were revealed, based on cox1 and cytb sequence variability. The simultaneous occurrence of Korea and Serbia 1 haplotypes was observed at five localities, whereas Peshter 1 haplotype was identifed at only one place.",
publisher = "Springer, Dordrecht",
journal = "Experimental and Applied Acarology",
title = "Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis",
volume = "61",
number = "1",
pages = "97-105",
doi = "10.1007/s10493-013-9683-9"
}

Gajić, B., Radulović, Ž., Stevanović, J., Kulišić, Z., Vučićević, M., Simeunović, P.,& Stanimirović, Z.. (2013). Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis. in Experimental and Applied Acarology
Springer, Dordrecht., 61(1), 97-105.
https://doi.org/10.1007/s10493-013-9683-9

Gajić B, Radulović Ž, Stevanović J, Kulišić Z, Vučićević M, Simeunović P, Stanimirović Z. Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis. in Experimental and Applied Acarology. 2013;61(1):97-105.
doi:10.1007/s10493-013-9683-9 .

Gajić, Bojan, Radulović, Željko, Stevanović, Jevrosima, Kulišić, Zoran, Vučićević, Miloš, Simeunović, Predrag, Stanimirović, Zoran, "Variability of the honey bee mite Varroa destructor in Serbia, based on mtDNA analysis" in Experimental and Applied Acarology, 61, no. 1 (2013):97-105,
https://doi.org/10.1007/s10493-013-9683-9 . .