TY  - JOUR
AU  - Debeljak, Zoran
AU  - Boufana, Belgees
AU  - Interisano, Maria
AU  - Vidanović, Dejan
AU  - Kulišić, Zoran
AU  - Casulli, Adriano
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1404
AB  - Cystic echinococcosis (CE) caused by Echinococcus granulosus sensu stricto (s.s.) is a cosmopolitan zoonotic infection which is endemic in Serbia where it is subject to mandatory reporting. However, information on the incidence of the disease in humans and prevalence of hydatid infection in livestock remains limited. We used sequenced data of the cytochrome c oxidase subunit 1 (cox 1) mitochondrial gene to examine the genetic diversity and population structure of E. granulosus (s.s.) from intermediate hosts from Serbia. We also compared our generated nucleotide sequences with those reported for neighbouring European countries. Echinococcus canadensis was molecularly confirmed from pig and human hydatid isolates. E. granulosus (G1) was confirmed from sheep and cattle hydatid isolates as well as the first molecular confirmation in Serbia of E. granulosus G2 in sheep and E. granulosus G3 in sheep and cattle hydatid isolates. The Serbian E. granulosus (s.s.) parsimony network displayed 2 main haplotypes (SB02 and SB05) which together with the neutrality indices were suggestive of bottleneck and/or balancing selection. Haplotype analysis showed the presence of the common E. granulosus haplotype described from other worldwide regions. Investigation of the pairwise fixation (Fst) index suggested that Serbian populations of E. granulosus (s.s.) from sheep and cattle hosts showed moderate genetic differentiation. Six of the Serbian haplotypes (SB02-SB07) were shared with haplotypes from Bulgaria, Hungary and/or Romania. Further studies using a larger number of hydatid isolates from various locations across Serbia will provide more information on the genetic structure of E. granulosus (s.s.) within this region.
PB  - Elsevier Science Bv, Amsterdam
T2  - Veterinary Parasitology
T1  - First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia
VL  - 223
SP  - 57
EP  - 62
DO  - 10.1016/j.vetpar.2016.04.007
ER  - 

@article{
author = "Debeljak, Zoran and Boufana, Belgees and Interisano, Maria and Vidanović, Dejan and Kulišić, Zoran and Casulli, Adriano",
year = "2016",
abstract = "Cystic echinococcosis (CE) caused by Echinococcus granulosus sensu stricto (s.s.) is a cosmopolitan zoonotic infection which is endemic in Serbia where it is subject to mandatory reporting. However, information on the incidence of the disease in humans and prevalence of hydatid infection in livestock remains limited. We used sequenced data of the cytochrome c oxidase subunit 1 (cox 1) mitochondrial gene to examine the genetic diversity and population structure of E. granulosus (s.s.) from intermediate hosts from Serbia. We also compared our generated nucleotide sequences with those reported for neighbouring European countries. Echinococcus canadensis was molecularly confirmed from pig and human hydatid isolates. E. granulosus (G1) was confirmed from sheep and cattle hydatid isolates as well as the first molecular confirmation in Serbia of E. granulosus G2 in sheep and E. granulosus G3 in sheep and cattle hydatid isolates. The Serbian E. granulosus (s.s.) parsimony network displayed 2 main haplotypes (SB02 and SB05) which together with the neutrality indices were suggestive of bottleneck and/or balancing selection. Haplotype analysis showed the presence of the common E. granulosus haplotype described from other worldwide regions. Investigation of the pairwise fixation (Fst) index suggested that Serbian populations of E. granulosus (s.s.) from sheep and cattle hosts showed moderate genetic differentiation. Six of the Serbian haplotypes (SB02-SB07) were shared with haplotypes from Bulgaria, Hungary and/or Romania. Further studies using a larger number of hydatid isolates from various locations across Serbia will provide more information on the genetic structure of E. granulosus (s.s.) within this region.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Veterinary Parasitology",
title = "First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia",
volume = "223",
pages = "57-62",
doi = "10.1016/j.vetpar.2016.04.007"
}

Debeljak, Z., Boufana, B., Interisano, M., Vidanović, D., Kulišić, Z.,& Casulli, A.. (2016). First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia. in Veterinary Parasitology
Elsevier Science Bv, Amsterdam., 223, 57-62.
https://doi.org/10.1016/j.vetpar.2016.04.007

Debeljak Z, Boufana B, Interisano M, Vidanović D, Kulišić Z, Casulli A. First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia. in Veterinary Parasitology. 2016;223:57-62.
doi:10.1016/j.vetpar.2016.04.007 .

Debeljak, Zoran, Boufana, Belgees, Interisano, Maria, Vidanović, Dejan, Kulišić, Zoran, Casulli, Adriano, "First insights into the genetic diversity of Echinococcus granulosus sensu stricto (s.s.) in Serbia" in Veterinary Parasitology, 223 (2016):57-62,
https://doi.org/10.1016/j.vetpar.2016.04.007 . .

TY  - CONF
AU  - Zivojinović, M.
AU  - Sofronić-Milosavljević, Ljiljana
AU  - Cvetković, J.
AU  - Pozio, E.
AU  - Interisano, M.
AU  - Plavsić, B.
AU  - Radojičić, Sonja
AU  - Kulišić, Zoran
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1023
AB  - Trichinella infections are endemic in the Balkan region of Europe. Though trichinellosis and agents thereof are serious problems for human health and animal husbandry, only a limited number of Trichinella isolates from Serbia have been identified at the species level so far. The aim of the present study was the surveillance and monitoring of Trichinella in domestic pigs and wild animals from the endemic district of Branicevo. Investigations performed during the 2009-2010 period revealed Trichinella infections in 344 out of 282,960(0.12%) domestic pigs. Among wildlife, Trichinella infections were detected in 11 out of 94 (11.7%) wild boars (Sus scrofa), 7 out of 57 (12.3%) red foxes (Vulpes vulpes), 7 out of 13 (53.8%) golden jackals (Canis aureus), and in all three examined wolves (Canis lupus). Trichinella spiralis and Trichinella britovi were the only two species identified. T. britovi was identified in 31% of isolates from wildlife of the Branicevo district and T. spiralis was found in 53% of wild animals; mixed infections were observed in 16% of the animals examined. Findings form the basis of an information campaign for veterinary services, pig owners and the hunters associations about the risk of the transmission of these zoonotic agents. The application of control programs as established at the Veterinary Specialist Institute of Pozarevac resulted in a decline in Trichinella infections among domestic pigs and the absence of human trichinellosis in the last three years in the Branicevo district.
PB  - Elsevier Science Bv, Amsterdam
C3  - Veterinary Parasitology
T1  - Trichinella infections in different host species of an endemic district of Serbia
VL  - 194
IS  - 2-4
SP  - 136
EP  - 138
DO  - 10.1016/j.vetpar.2013.01.039
ER  - 

@conference{
author = "Zivojinović, M. and Sofronić-Milosavljević, Ljiljana and Cvetković, J. and Pozio, E. and Interisano, M. and Plavsić, B. and Radojičić, Sonja and Kulišić, Zoran",
year = "2013",
abstract = "Trichinella infections are endemic in the Balkan region of Europe. Though trichinellosis and agents thereof are serious problems for human health and animal husbandry, only a limited number of Trichinella isolates from Serbia have been identified at the species level so far. The aim of the present study was the surveillance and monitoring of Trichinella in domestic pigs and wild animals from the endemic district of Branicevo. Investigations performed during the 2009-2010 period revealed Trichinella infections in 344 out of 282,960(0.12%) domestic pigs. Among wildlife, Trichinella infections were detected in 11 out of 94 (11.7%) wild boars (Sus scrofa), 7 out of 57 (12.3%) red foxes (Vulpes vulpes), 7 out of 13 (53.8%) golden jackals (Canis aureus), and in all three examined wolves (Canis lupus). Trichinella spiralis and Trichinella britovi were the only two species identified. T. britovi was identified in 31% of isolates from wildlife of the Branicevo district and T. spiralis was found in 53% of wild animals; mixed infections were observed in 16% of the animals examined. Findings form the basis of an information campaign for veterinary services, pig owners and the hunters associations about the risk of the transmission of these zoonotic agents. The application of control programs as established at the Veterinary Specialist Institute of Pozarevac resulted in a decline in Trichinella infections among domestic pigs and the absence of human trichinellosis in the last three years in the Branicevo district.",
publisher = "Elsevier Science Bv, Amsterdam",
journal = "Veterinary Parasitology",
title = "Trichinella infections in different host species of an endemic district of Serbia",
volume = "194",
number = "2-4",
pages = "136-138",
doi = "10.1016/j.vetpar.2013.01.039"
}

Zivojinović, M., Sofronić-Milosavljević, L., Cvetković, J., Pozio, E., Interisano, M., Plavsić, B., Radojičić, S.,& Kulišić, Z.. (2013). Trichinella infections in different host species of an endemic district of Serbia. in Veterinary Parasitology
Elsevier Science Bv, Amsterdam., 194(2-4), 136-138.
https://doi.org/10.1016/j.vetpar.2013.01.039

Zivojinović M, Sofronić-Milosavljević L, Cvetković J, Pozio E, Interisano M, Plavsić B, Radojičić S, Kulišić Z. Trichinella infections in different host species of an endemic district of Serbia. in Veterinary Parasitology. 2013;194(2-4):136-138.
doi:10.1016/j.vetpar.2013.01.039 .

Zivojinović, M., Sofronić-Milosavljević, Ljiljana, Cvetković, J., Pozio, E., Interisano, M., Plavsić, B., Radojičić, Sonja, Kulišić, Zoran, "Trichinella infections in different host species of an endemic district of Serbia" in Veterinary Parasitology, 194, no. 2-4 (2013):136-138,
https://doi.org/10.1016/j.vetpar.2013.01.039 . .