TY  - JOUR
AU  - Kos, Andrea
AU  - Papić, Bojan
AU  - Golob, Majda
AU  - Avberšek, Jana
AU  - Kušar, Darja
AU  - Ledina, Tijana
AU  - Đorđević, Jasna
AU  - Bulajić, Snežana
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3429
AB  - The potential risk to human and animal health provides a rationale for research on
methicillin-resistant staphylococci (MRS) and mammaliicocci (MRM) in dairy herds. Here, we
aimed to estimate their occurrence in the bulk tank milk (BTM) samples collected in 2019–2021 from
283 bovine dairy farms in the Belgrade district. We used whole-genome sequencing to characterize
the obtained isolates and assess their genetic relatedness. A total of 70 MRS/MRM were recovered,
most frequently Staphylococcus haemolyticus and Mammaliicoccus sciuri. Five clusters of 2–4 genetically
related isolates were identified and epidemiological data indicated transmission through, e.g., farm
visits by personnel or milk collection trucks. Most MRSA isolates belonged to the typical livestockassociated
lineage ST398-t034. One MRSA isolate (ST152-t355) harbored the PVL-encoding genes.
Since MRS/MRM isolates obtained in this study frequently harbored genes conferring multidrug
resistance (MDR), this argues for their role as reservoirs for the spread of antimicrobial resistance
genes. The pipeline milking system and total bacterial count >100,000 CFU/mL were significantly
associated with higher occurrences of MRS/MRM. Our study confirms that BTM can be a zoonotic
source of MRS, including MDR strains. This highlights the urgent need for good agricultural practices
and the continuous monitoring of MRS/MRM in dairy farms.
PB  - MDPI
T2  - Antibiotics
T1  - Genomic insights into methicillin-resistant staphylococci and mammaliicocci from bulk tank milk of dairy farms in Serbia
VL  - 12
IS  - 10
SP  - 1529
DO  - 10.3390/antibiotics12101529
ER  - 

@article{
author = "Kos, Andrea and Papić, Bojan and Golob, Majda and Avberšek, Jana and Kušar, Darja and Ledina, Tijana and Đorđević, Jasna and Bulajić, Snežana",
year = "2023",
abstract = "The potential risk to human and animal health provides a rationale for research on
methicillin-resistant staphylococci (MRS) and mammaliicocci (MRM) in dairy herds. Here, we
aimed to estimate their occurrence in the bulk tank milk (BTM) samples collected in 2019–2021 from
283 bovine dairy farms in the Belgrade district. We used whole-genome sequencing to characterize
the obtained isolates and assess their genetic relatedness. A total of 70 MRS/MRM were recovered,
most frequently Staphylococcus haemolyticus and Mammaliicoccus sciuri. Five clusters of 2–4 genetically
related isolates were identified and epidemiological data indicated transmission through, e.g., farm
visits by personnel or milk collection trucks. Most MRSA isolates belonged to the typical livestockassociated
lineage ST398-t034. One MRSA isolate (ST152-t355) harbored the PVL-encoding genes.
Since MRS/MRM isolates obtained in this study frequently harbored genes conferring multidrug
resistance (MDR), this argues for their role as reservoirs for the spread of antimicrobial resistance
genes. The pipeline milking system and total bacterial count >100,000 CFU/mL were significantly
associated with higher occurrences of MRS/MRM. Our study confirms that BTM can be a zoonotic
source of MRS, including MDR strains. This highlights the urgent need for good agricultural practices
and the continuous monitoring of MRS/MRM in dairy farms.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Genomic insights into methicillin-resistant staphylococci and mammaliicocci from bulk tank milk of dairy farms in Serbia",
volume = "12",
number = "10",
pages = "1529",
doi = "10.3390/antibiotics12101529"
}

Kos, A., Papić, B., Golob, M., Avberšek, J., Kušar, D., Ledina, T., Đorđević, J.,& Bulajić, S.. (2023). Genomic insights into methicillin-resistant staphylococci and mammaliicocci from bulk tank milk of dairy farms in Serbia. in Antibiotics
MDPI., 12(10), 1529.
https://doi.org/10.3390/antibiotics12101529

Kos A, Papić B, Golob M, Avberšek J, Kušar D, Ledina T, Đorđević J, Bulajić S. Genomic insights into methicillin-resistant staphylococci and mammaliicocci from bulk tank milk of dairy farms in Serbia. in Antibiotics. 2023;12(10):1529.
doi:10.3390/antibiotics12101529 .

Kos, Andrea, Papić, Bojan, Golob, Majda, Avberšek, Jana, Kušar, Darja, Ledina, Tijana, Đorđević, Jasna, Bulajić, Snežana, "Genomic insights into methicillin-resistant staphylococci and mammaliicocci from bulk tank milk of dairy farms in Serbia" in Antibiotics, 12, no. 10 (2023):1529,
https://doi.org/10.3390/antibiotics12101529 . .

TY  - JOUR
AU  - Đorđević, Jasna
AU  - Ledina, Tijana
AU  - Golob, Majda
AU  - Mohar Lorbeg, Petra
AU  - Čanžek Majhenič, Andreja
AU  - Bogovič Matijašić, Bojana
AU  - Bulajić, Snežana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2713
AB  - Enterococci represent a significant part of the non-starter LAB microbiota of artisanal cheeses produced mainly from raw milk. Common approaches to safety evaluation of enterococci isolates include assessment of antimicrobial resistance and virulence potential. Hence, a collection of 47 (n = 22, Serbia; n = 25, Slovenia) dairy enterococcal isolates, of which E. faecalis (n = 28), E. faecium (n = 11), E. durans (n = 5), E. casseliflavus (n = 2), and E. gallinarum (n = 1), was analyzed. The susceptibility to 12 antimicrobials was tested using a broth microdilution method, and the presence of the selected antimicrobial resistance and virulence genes was investigated using PCR. Isolates were resistant to tetracycline (TET) (25.5%), erythromycin (ERY) (17.0%), gentamycin and chloramphenicol (CHL) (∼6%). No resistance to ampicillin (AMP), ciprofloxacin (CIP), daptomycin (DAP), linezolid (LZD), teicoplanin (TEI), tigecycline (TGC) and vancomycin (VAN) was detected. Among all the resistance determinants analyzed, ermB gene was detected most frequently. All 10 virulence genes analyzed were detected with a distribution of cpd (72.3%), cob and ccf (70.2%), gelE (68.1%), hyl (59.6%), agg (53.2%) and esp (46.8%). The genes encoding cytolysin (cylA, cylM and cylB) were amplified to a lesser extent (21.3%, 21.3% and 12.8%, respectively). However, due to the limited number of enterococci isolates analyzed in the present study, further studies are still required in order to better document the safety status of dairy enterococci.
PB  - SAGE Publications
T2  - Food Science and Technology International
T1  - Safety evaluation of enterococci isolated from raw milk and artisanal cheeses made in Slovenia and Serbia
SP  - 1
EP  - 11
DO  - 10.1177/10820132221117870
ER  - 

@article{
author = "Đorđević, Jasna and Ledina, Tijana and Golob, Majda and Mohar Lorbeg, Petra and Čanžek Majhenič, Andreja and Bogovič Matijašić, Bojana and Bulajić, Snežana",
year = "2022",
abstract = "Enterococci represent a significant part of the non-starter LAB microbiota of artisanal cheeses produced mainly from raw milk. Common approaches to safety evaluation of enterococci isolates include assessment of antimicrobial resistance and virulence potential. Hence, a collection of 47 (n = 22, Serbia; n = 25, Slovenia) dairy enterococcal isolates, of which E. faecalis (n = 28), E. faecium (n = 11), E. durans (n = 5), E. casseliflavus (n = 2), and E. gallinarum (n = 1), was analyzed. The susceptibility to 12 antimicrobials was tested using a broth microdilution method, and the presence of the selected antimicrobial resistance and virulence genes was investigated using PCR. Isolates were resistant to tetracycline (TET) (25.5%), erythromycin (ERY) (17.0%), gentamycin and chloramphenicol (CHL) (∼6%). No resistance to ampicillin (AMP), ciprofloxacin (CIP), daptomycin (DAP), linezolid (LZD), teicoplanin (TEI), tigecycline (TGC) and vancomycin (VAN) was detected. Among all the resistance determinants analyzed, ermB gene was detected most frequently. All 10 virulence genes analyzed were detected with a distribution of cpd (72.3%), cob and ccf (70.2%), gelE (68.1%), hyl (59.6%), agg (53.2%) and esp (46.8%). The genes encoding cytolysin (cylA, cylM and cylB) were amplified to a lesser extent (21.3%, 21.3% and 12.8%, respectively). However, due to the limited number of enterococci isolates analyzed in the present study, further studies are still required in order to better document the safety status of dairy enterococci.",
publisher = "SAGE Publications",
journal = "Food Science and Technology International",
title = "Safety evaluation of enterococci isolated from raw milk and artisanal cheeses made in Slovenia and Serbia",
pages = "1-11",
doi = "10.1177/10820132221117870"
}

Đorđević, J., Ledina, T., Golob, M., Mohar Lorbeg, P., Čanžek Majhenič, A., Bogovič Matijašić, B.,& Bulajić, S.. (2022). Safety evaluation of enterococci isolated from raw milk and artisanal cheeses made in Slovenia and Serbia. in Food Science and Technology International
SAGE Publications., 1-11.
https://doi.org/10.1177/10820132221117870

Đorđević J, Ledina T, Golob M, Mohar Lorbeg P, Čanžek Majhenič A, Bogovič Matijašić B, Bulajić S. Safety evaluation of enterococci isolated from raw milk and artisanal cheeses made in Slovenia and Serbia. in Food Science and Technology International. 2022;:1-11.
doi:10.1177/10820132221117870 .

Đorđević, Jasna, Ledina, Tijana, Golob, Majda, Mohar Lorbeg, Petra, Čanžek Majhenič, Andreja, Bogovič Matijašić, Bojana, Bulajić, Snežana, "Safety evaluation of enterococci isolated from raw milk and artisanal cheeses made in Slovenia and Serbia" in Food Science and Technology International (2022):1-11,
https://doi.org/10.1177/10820132221117870 . .