TY  - JOUR
AU  - Kosanović, Maja
AU  - Cvetković, Jelena
AU  - Gruden-Movsesijan, Alisa
AU  - Vasilev, Saša
AU  - Milanović, Svetlana
AU  - Ilić, Natasa
AU  - Sofronic-Milosavljević, Ljiljana
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1785
AB  - Aims Extracellular vesicles (EVs) represent a newly discovered but universal communication tool between cells or organisms. However, few data exist on nematode EVs and none for Trichinella spiralis. Here, we aimed to investigate whether T spiralis muscle larvae produce EVs, whether they carry immunomodulatory proteins and whether they have a role in immunomodulation as a component of excretory-secretory muscle larvae products (ES L1). Methods and results EVs were enriched from conditioned medium of T spiralis muscle larvae. Transmission electron microscopy images showed T spiralis EVs to be 30-80 nm in size, and Western blot confirmed the presence of two out of three glycoproteins with the immunodominant epitope characteristic for muscle larvae of the genus Trichinella. Using a peripheral blood mononuclear cell (PBMC) stimulation assay, it was shown that these EVs elevated production of IL10 and IL6. Conclusion T spiralis muscle larvae produce EVs. Those EVs carry immunomodulatory proteins and have the capacity independently to induce regulatory responses in the same way as the T spiralis excretory-secretory muscle larvae products from which they were isolated.
PB  - Wiley, Hoboken
T2  - Parasite Immunology
T1  - Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties
VL  - 41
IS  - 10
SP  - e12665
DO  - 10.1111/pim.12665
ER  - 

@article{
author = "Kosanović, Maja and Cvetković, Jelena and Gruden-Movsesijan, Alisa and Vasilev, Saša and Milanović, Svetlana and Ilić, Natasa and Sofronic-Milosavljević, Ljiljana",
year = "2019",
abstract = "Aims Extracellular vesicles (EVs) represent a newly discovered but universal communication tool between cells or organisms. However, few data exist on nematode EVs and none for Trichinella spiralis. Here, we aimed to investigate whether T spiralis muscle larvae produce EVs, whether they carry immunomodulatory proteins and whether they have a role in immunomodulation as a component of excretory-secretory muscle larvae products (ES L1). Methods and results EVs were enriched from conditioned medium of T spiralis muscle larvae. Transmission electron microscopy images showed T spiralis EVs to be 30-80 nm in size, and Western blot confirmed the presence of two out of three glycoproteins with the immunodominant epitope characteristic for muscle larvae of the genus Trichinella. Using a peripheral blood mononuclear cell (PBMC) stimulation assay, it was shown that these EVs elevated production of IL10 and IL6. Conclusion T spiralis muscle larvae produce EVs. Those EVs carry immunomodulatory proteins and have the capacity independently to induce regulatory responses in the same way as the T spiralis excretory-secretory muscle larvae products from which they were isolated.",
publisher = "Wiley, Hoboken",
journal = "Parasite Immunology",
title = "Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties",
volume = "41",
number = "10",
pages = "e12665",
doi = "10.1111/pim.12665"
}

Kosanović, M., Cvetković, J., Gruden-Movsesijan, A., Vasilev, S., Milanović, S., Ilić, N.,& Sofronic-Milosavljević, L.. (2019). Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties. in Parasite Immunology
Wiley, Hoboken., 41(10), e12665.
https://doi.org/10.1111/pim.12665

Kosanović M, Cvetković J, Gruden-Movsesijan A, Vasilev S, Milanović S, Ilić N, Sofronic-Milosavljević L. Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties. in Parasite Immunology. 2019;41(10):e12665.
doi:10.1111/pim.12665 .

Kosanović, Maja, Cvetković, Jelena, Gruden-Movsesijan, Alisa, Vasilev, Saša, Milanović, Svetlana, Ilić, Natasa, Sofronic-Milosavljević, Ljiljana, "Trichinella spiralis muscle larvae release extracellular vesicles with immunomodulatory properties" in Parasite Immunology, 41, no. 10 (2019):e12665,
https://doi.org/10.1111/pim.12665 . .