TY  - JOUR
AU  - Tešić, M.
AU  - Nedić, Drago
AU  - Baltić, Milan Ž.
AU  - Božić, Dragica
AU  - Stojiljković, Lj.
AU  - Plavšić, B.
AU  - Tajdić, Nada
AU  - Mirilović, Milorad
AU  - Rajković, M.
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/819
AB  - Trichinellosis is a disease that affects both humans and animals, caused by a parasite from the Trichinellidae family and Trichinella genus. Humans get infected by consuming infected and inadequately thermally treated meat from domestic or wild animals containing cocooned infective larvae of T. spiralis. Aside from health problems caused by trichinellosis as a zoonosis, there is no doubt that it represents a serious economic problem for swine meat producers. The research in this paper has been performed in Serbia, in a region which is located at the confluence of two large rivers, and it is the geographical location which makes it an endemic area for the presence of T. spiralis. Epidemiological data pertains to the period of 1995-2003, but also to the period 2003-2009, when the implementation of the program for control and eradication of trichinellosis was complete. The diagnostic testing of cadavers of slaughtered swine for the presence of T. spiralis in the period of 1995-2003 included 41.04% of the slaughtered swine, out of which 0.4281% were positive, and the amount of economic loss was 95301000 dinars or about one million EUR. The infection was confirmed in 432 patients during the research period. The preparation of the program for the control and eradication of trichinellosis has been made in accordance with applicable laws and technological standards, with a clear determination of input and output using a cost-benefit analysis. The effects of its application show a reduction in the number of swine which are positive for the presence of T. spiralis by a factor of three times (p lt 0.01), and the present net value (PNV) and the benefit/cost ratio (B/C) show economic and epidemiological justification.
AB  - Trihineloza je zajedničko oboljenje ljudi i životinja koje izaziva parazit koji pripada familiji Trichinellidae i rodu Trichinella. Čovek se zarazi tako što konzumira zaraženo, nedovoljno termički obrađeno meso domaćih i divljih životinja u kojima se nalazi učaurena infektivna larva T. spiralis. Pored zdravstvenih problema koje izaziva trihineloza kao zoonoza, nesumljivo je da ona predstavlja i ozbiljan ekonomski problem za proizvođača svinjskog mesa. Ova istraživanja su izvršena u Srbiji na teritoriji jednog regiona koji se nalazi u slivu dve velike reke, i zbog takvog geografskog položaja predstavlja endemsko područje za prisustvo T. spiralis. Epidemiološki podaci se odnose na period 1995-2003. godine, kao i na period 2003- 2009. godine kada je izvršena implementacija programa kontrole i eradikacije trihineloze. Dijagnostičkim ispitivanjem trupova zaklanih svinja na prisustvo T. spiralis u periodu 1995-2003. godina obuhvaćeno je 41.04% zaklanih svinja od čega je bilo 0.4281% pozitivnih svinja, a visina ekonomskih gubitaka iznosila je 95 301 000 dinara ili oko milion EUR-a. U toku posmatranog perioda obolele su 432 osobe. Izrada programa za kontrolu i eradikaciju trihineloze izvršena je na osnovu važećih zakona i tehnoloških standarda, uz jasnu determinaciju 'inputa' i 'outputa' pomoću 'cost-benefit' analize. Efekti njegove primene pokazuju smanjenje broja pozitivnih svinja na prisustvo T. spiralis za tri puta (p lt 0,01), a neto sadašnja vrednost (PNV) i odnos koristi i troškova (B/C) pokazuju ekonomsku i epidemiološku opravdanost.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effects of the application of trichinellosis control program in an endemic area in Serbia
T1  - Efekti primene programa kontrole trihineloze na endemskom području u Srbiji
VL  - 61
IS  - 1
SP  - 77
EP  - 87
DO  - 10.2298/AVB1101077T
ER  - 

@article{
author = "Tešić, M. and Nedić, Drago and Baltić, Milan Ž. and Božić, Dragica and Stojiljković, Lj. and Plavšić, B. and Tajdić, Nada and Mirilović, Milorad and Rajković, M.",
year = "2011",
abstract = "Trichinellosis is a disease that affects both humans and animals, caused by a parasite from the Trichinellidae family and Trichinella genus. Humans get infected by consuming infected and inadequately thermally treated meat from domestic or wild animals containing cocooned infective larvae of T. spiralis. Aside from health problems caused by trichinellosis as a zoonosis, there is no doubt that it represents a serious economic problem for swine meat producers. The research in this paper has been performed in Serbia, in a region which is located at the confluence of two large rivers, and it is the geographical location which makes it an endemic area for the presence of T. spiralis. Epidemiological data pertains to the period of 1995-2003, but also to the period 2003-2009, when the implementation of the program for control and eradication of trichinellosis was complete. The diagnostic testing of cadavers of slaughtered swine for the presence of T. spiralis in the period of 1995-2003 included 41.04% of the slaughtered swine, out of which 0.4281% were positive, and the amount of economic loss was 95301000 dinars or about one million EUR. The infection was confirmed in 432 patients during the research period. The preparation of the program for the control and eradication of trichinellosis has been made in accordance with applicable laws and technological standards, with a clear determination of input and output using a cost-benefit analysis. The effects of its application show a reduction in the number of swine which are positive for the presence of T. spiralis by a factor of three times (p lt 0.01), and the present net value (PNV) and the benefit/cost ratio (B/C) show economic and epidemiological justification., Trihineloza je zajedničko oboljenje ljudi i životinja koje izaziva parazit koji pripada familiji Trichinellidae i rodu Trichinella. Čovek se zarazi tako što konzumira zaraženo, nedovoljno termički obrađeno meso domaćih i divljih životinja u kojima se nalazi učaurena infektivna larva T. spiralis. Pored zdravstvenih problema koje izaziva trihineloza kao zoonoza, nesumljivo je da ona predstavlja i ozbiljan ekonomski problem za proizvođača svinjskog mesa. Ova istraživanja su izvršena u Srbiji na teritoriji jednog regiona koji se nalazi u slivu dve velike reke, i zbog takvog geografskog položaja predstavlja endemsko područje za prisustvo T. spiralis. Epidemiološki podaci se odnose na period 1995-2003. godine, kao i na period 2003- 2009. godine kada je izvršena implementacija programa kontrole i eradikacije trihineloze. Dijagnostičkim ispitivanjem trupova zaklanih svinja na prisustvo T. spiralis u periodu 1995-2003. godina obuhvaćeno je 41.04% zaklanih svinja od čega je bilo 0.4281% pozitivnih svinja, a visina ekonomskih gubitaka iznosila je 95 301 000 dinara ili oko milion EUR-a. U toku posmatranog perioda obolele su 432 osobe. Izrada programa za kontrolu i eradikaciju trihineloze izvršena je na osnovu važećih zakona i tehnoloških standarda, uz jasnu determinaciju 'inputa' i 'outputa' pomoću 'cost-benefit' analize. Efekti njegove primene pokazuju smanjenje broja pozitivnih svinja na prisustvo T. spiralis za tri puta (p lt 0,01), a neto sadašnja vrednost (PNV) i odnos koristi i troškova (B/C) pokazuju ekonomsku i epidemiološku opravdanost.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effects of the application of trichinellosis control program in an endemic area in Serbia, Efekti primene programa kontrole trihineloze na endemskom području u Srbiji",
volume = "61",
number = "1",
pages = "77-87",
doi = "10.2298/AVB1101077T"
}

Tešić, M., Nedić, D., Baltić, M. Ž., Božić, D., Stojiljković, Lj., Plavšić, B., Tajdić, N., Mirilović, M.,& Rajković, M.. (2011). Effects of the application of trichinellosis control program in an endemic area in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(1), 77-87.
https://doi.org/10.2298/AVB1101077T

Tešić M, Nedić D, Baltić MŽ, Božić D, Stojiljković L, Plavšić B, Tajdić N, Mirilović M, Rajković M. Effects of the application of trichinellosis control program in an endemic area in Serbia. in Acta Veterinaria-Beograd. 2011;61(1):77-87.
doi:10.2298/AVB1101077T .

Tešić, M., Nedić, Drago, Baltić, Milan Ž., Božić, Dragica, Stojiljković, Lj., Plavšić, B., Tajdić, Nada, Mirilović, Milorad, Rajković, M., "Effects of the application of trichinellosis control program in an endemic area in Serbia" in Acta Veterinaria-Beograd, 61, no. 1 (2011):77-87,
https://doi.org/10.2298/AVB1101077T . .

TY  - JOUR
AU  - Nedić, Drago
AU  - Tešić, M.
AU  - Baltić, Milan Ž.
AU  - Plavšić, B.
AU  - Tajdić, Nada
AU  - Mirilović, Milorad
AU  - Rajković, M.
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/806
AB  - Classical swine fever (CSF) has been present in Serbia for a long period of time as an endemic disease, and it occurs with lesser or greater intensity from year to year. Since it is a highly contagious disease of domestic and wild pigs, the outbreaks of the disease causes severe consequences for animal welfare, livestock production and economic losses to the national economies in countries where it occurs. Therefore, CSF has a great epizootiological and economic importance to the swine production, primarily because of trade restrictions for live pigs and pork products. CSF eradication and control programmes are generally based on vaccination strategies in countries with endemic occurrence of CSF or non-vaccination policies in countries which successfully eradicated or are free of CSF. Whatever the general eradication strategy is in place, in the case of an outbreak of CSF the stamping-out method is used for suppression and eradication of the disease, with strict implementation of specific veterinary-sanitary measures in the infected and surveillance zones, clearly defined by legal provisions. In the period before 2006, there were certain regions in Serbia where CSF occurred almost every year, including regions where pig farming was not the primary branch of animal husbandry. Pig identification and implementation of vaccination, as well as the control of trade of pigs from infected areas to other parts of the country were not very successful. Due to the serious losses inflicted by the disease, a new Programme of CSF control was adopted in 2006, which inter alia included the obligatory identification of pigs, registration of pig holdings and free of charge vaccination of all susceptible animals in the country, as well as the implementation of other statutory measures, including obligatory evidence of veterinary activities in the national Veterinary information system. The results obtained in the period after the implementation of the Programme of CSF control (2007, 2008 and 2009) indicate a positive impact on the reduction of outbreaks of CSF, as well as on the reduction of prevalence (p lt 0.001). Also, the effectiveness of vaccination of pigs in 2007 compared to the period before and after the implementation of the Programme was the highest (97.78%), as well as the costs of its implementation.
AB  - Klasična kuga svinja (KKS) je prisutna u Srbiji prisutna duži vremenski period i sa manjim ili većim intenzitetom javlja se iz godine u godinu. Kako je u pitanju infektivna bolest svinja, a polazeći od karaktera njenog širenja sama pojava bolesti nanosi velike ekonomske štete za privredu svake zemlje. Stoga KKS ima veliki epizootiološki i ekonomski značaj za svinjarsku proizvodnju, a pre svega za stvaranje nesmetane mogućnosti odvijanja prometa svinja i proizvoda od svinjskog mesa. Program kontrole KKS se može izvoditi vakcinacijom ili biti bez nje. Kod pojave bolesti se i u jednom i drugom slučaju za suzbijanje i iskorenjivanje primenjuje metod 'stamping-out', i sprovode se određene veterinarsko-sanitarne mere u zaraženom i ugroženom dvorištu koje su decidno definisane zakonskim propisima. U proteklom periodu u Srbiji su postojali određeni regioni gde se KKS javljala skoro svake godine, a naročito je bila zabrinjavajuća pojava širenja bolesti u regione gde svinjarstvo nije primarna grana stočarstva. Kontrola prometa svinja iz zaraženih područja u druge delove zemlje bila je na niskom nivou. Zbog ozbiljnih gubitaka koje je nanosila pojava bolesti, donet je novi Program kontrole KKS koji između ostalog podrazumeva obavezno obeležavanje svinja i besplatnu vakcinaciju svih prijemčivih životinja u zemlji, kao i sprovođenje drugih zakonom propisanih mera. Dobijeni rezultati u periodu posle sprovođenja Programa kontrole KKS (2008. i 2009. godine) ukazuju na pozitivan uticaj ovih mera na smanjenje pojave žarišta KKS, kao i na smanjenje prevalance (p lt 0,001). Takođe, efikasnost sprovođenja vakcinacije svinja bila je najveća (97,78%) u 2007. godini, u odnosu na period pre i posle sprovođenja Programa.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Management and control program for suppression and eradication of classical swine fever in Serbia
T1  - Menadžment i kontrola programa suzbijanja i eradikacije klasične kuge svinja u Srbiji
VL  - 61
IS  - 2-3
SP  - 295
EP  - 307
DO  - 10.2298/AVB1103295N
ER  - 

@article{
author = "Nedić, Drago and Tešić, M. and Baltić, Milan Ž. and Plavšić, B. and Tajdić, Nada and Mirilović, Milorad and Rajković, M.",
year = "2011",
abstract = "Classical swine fever (CSF) has been present in Serbia for a long period of time as an endemic disease, and it occurs with lesser or greater intensity from year to year. Since it is a highly contagious disease of domestic and wild pigs, the outbreaks of the disease causes severe consequences for animal welfare, livestock production and economic losses to the national economies in countries where it occurs. Therefore, CSF has a great epizootiological and economic importance to the swine production, primarily because of trade restrictions for live pigs and pork products. CSF eradication and control programmes are generally based on vaccination strategies in countries with endemic occurrence of CSF or non-vaccination policies in countries which successfully eradicated or are free of CSF. Whatever the general eradication strategy is in place, in the case of an outbreak of CSF the stamping-out method is used for suppression and eradication of the disease, with strict implementation of specific veterinary-sanitary measures in the infected and surveillance zones, clearly defined by legal provisions. In the period before 2006, there were certain regions in Serbia where CSF occurred almost every year, including regions where pig farming was not the primary branch of animal husbandry. Pig identification and implementation of vaccination, as well as the control of trade of pigs from infected areas to other parts of the country were not very successful. Due to the serious losses inflicted by the disease, a new Programme of CSF control was adopted in 2006, which inter alia included the obligatory identification of pigs, registration of pig holdings and free of charge vaccination of all susceptible animals in the country, as well as the implementation of other statutory measures, including obligatory evidence of veterinary activities in the national Veterinary information system. The results obtained in the period after the implementation of the Programme of CSF control (2007, 2008 and 2009) indicate a positive impact on the reduction of outbreaks of CSF, as well as on the reduction of prevalence (p lt 0.001). Also, the effectiveness of vaccination of pigs in 2007 compared to the period before and after the implementation of the Programme was the highest (97.78%), as well as the costs of its implementation., Klasična kuga svinja (KKS) je prisutna u Srbiji prisutna duži vremenski period i sa manjim ili većim intenzitetom javlja se iz godine u godinu. Kako je u pitanju infektivna bolest svinja, a polazeći od karaktera njenog širenja sama pojava bolesti nanosi velike ekonomske štete za privredu svake zemlje. Stoga KKS ima veliki epizootiološki i ekonomski značaj za svinjarsku proizvodnju, a pre svega za stvaranje nesmetane mogućnosti odvijanja prometa svinja i proizvoda od svinjskog mesa. Program kontrole KKS se može izvoditi vakcinacijom ili biti bez nje. Kod pojave bolesti se i u jednom i drugom slučaju za suzbijanje i iskorenjivanje primenjuje metod 'stamping-out', i sprovode se određene veterinarsko-sanitarne mere u zaraženom i ugroženom dvorištu koje su decidno definisane zakonskim propisima. U proteklom periodu u Srbiji su postojali određeni regioni gde se KKS javljala skoro svake godine, a naročito je bila zabrinjavajuća pojava širenja bolesti u regione gde svinjarstvo nije primarna grana stočarstva. Kontrola prometa svinja iz zaraženih područja u druge delove zemlje bila je na niskom nivou. Zbog ozbiljnih gubitaka koje je nanosila pojava bolesti, donet je novi Program kontrole KKS koji između ostalog podrazumeva obavezno obeležavanje svinja i besplatnu vakcinaciju svih prijemčivih životinja u zemlji, kao i sprovođenje drugih zakonom propisanih mera. Dobijeni rezultati u periodu posle sprovođenja Programa kontrole KKS (2008. i 2009. godine) ukazuju na pozitivan uticaj ovih mera na smanjenje pojave žarišta KKS, kao i na smanjenje prevalance (p lt 0,001). Takođe, efikasnost sprovođenja vakcinacije svinja bila je najveća (97,78%) u 2007. godini, u odnosu na period pre i posle sprovođenja Programa.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Management and control program for suppression and eradication of classical swine fever in Serbia, Menadžment i kontrola programa suzbijanja i eradikacije klasične kuge svinja u Srbiji",
volume = "61",
number = "2-3",
pages = "295-307",
doi = "10.2298/AVB1103295N"
}

Nedić, D., Tešić, M., Baltić, M. Ž., Plavšić, B., Tajdić, N., Mirilović, M.,& Rajković, M.. (2011). Management and control program for suppression and eradication of classical swine fever in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(2-3), 295-307.
https://doi.org/10.2298/AVB1103295N

Nedić D, Tešić M, Baltić MŽ, Plavšić B, Tajdić N, Mirilović M, Rajković M. Management and control program for suppression and eradication of classical swine fever in Serbia. in Acta Veterinaria-Beograd. 2011;61(2-3):295-307.
doi:10.2298/AVB1103295N .

Nedić, Drago, Tešić, M., Baltić, Milan Ž., Plavšić, B., Tajdić, Nada, Mirilović, Milorad, Rajković, M., "Management and control program for suppression and eradication of classical swine fever in Serbia" in Acta Veterinaria-Beograd, 61, no. 2-3 (2011):295-307,
https://doi.org/10.2298/AVB1103295N . .

TY  - JOUR
AU  - Šekler, Milanko
AU  - Ašanin, Ružica
AU  - Krnjaić, Dejan
AU  - Palić, T.
AU  - Milić, Nenad
AU  - Jovanović, Tanja
AU  - Kovačević, Dragana
AU  - Plavšić, B.
AU  - Stojanović, Dragica
AU  - Vidanović, Dejan
AU  - Ašanin, N.
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/652
AB  - Infections caused by the avian influenza virus have been known for a long time and they are present, to a smaller or greater extent, in both extensive and intensive poultry production in many parts of the world. Epidemiological investigations have established a definite significance of the population of wild birds in maintaining and spreading this infection. Avian influenza is a zoonosis, and the virus has a great potential for causing mortality in humans, in particular its subtypes H5 and H7, which is why it has lately been provoking much attention among scientists and experts, as well as the general public. The objective of the work was to catch a certain number of wild birds in several locations in the Republic of Serbia, to identify them, and to collect samples of their blood serum for the determination of specific antibodies against the avian influenza virus. Birds were caught in ten locations in a manner that was safe for the birds themselves, as well as for the staff that did the catching. The birds were caught in especially produced nets, and in some cases in special traps. The caught wild birds were identified using the methods described in reference literature. All the names of the wild birds were coordinated with the valid Serbian nomenclature of European birds, prepared by prominent ornithologist and bird lover Milan Ružić. Following catching and identification, blood samples were taken from the birds from the wing vein (in bigger birds) or from the leg vein (in smaller birds). In taking blood samples from wild birds, all the principles of asepsis and antisepsis were followed in order to prevent any possibility of infection. After that, the birds were returned to their natural habitat, to the same locations in which they were caught. Serums were isolated from the taken blood samples and they were stored at -20ºC until the final examinations. A total of 46 species of wild birds were identified among a total of 259 birds from which 259 samples of blood serum were isolated. The following were used for the detection of the presence of specific antibodies against the avian influenza virus in blood serum samples of wild birds: agar gel precipitation (AGP), the hemagglutination inhibition test (HI) for subtypes H5 and H7, the cELISA test with antigen for the A type avian infleunza virus, and the cELISA test with antigen for subtype H5 of the avian influenza virus. Due to the fact that about 360 different species of wild birds live in the Republic of Serbia, the number of 46 identified species covered by these investigations account for 12.77% of the total number of bird species present in Serbia, which is considered a good sample. Specific antibodies against the A type avian influenza virus were established in serum samples of only 9 of the 259 birds covered by examinations using the cELISA test. Of the 46 identified wild bird species, 6 belonged to birds that live exclusively in water habitats and are considered a reservoir of the avian influenza virus (white stork, mallard, mute swan, common pochard, common goldeneye, and Eurasian coot). Among the listed species, particular attention was drawn to 4 species of wild birds of the order Anseriformes and the family Anatidae (mallard, mute swan, common pochard, common goldeneye) of which there were 30 birds among the total of 259 examined. In the 30 blood serum samples of the listed bird species, specific antibodies against the A type avian influenza virus were established in 9 (30%) serum samples using cELISA. Specific antibodies against the avian influenza virus subtype H5 were established in 3 serum samples of mute swans (one serum sample originated from a mute swan which was tagged in Poland) and in one blood serum sample of a common pochard, or a total of 4 (13.33%) serum samples, using the hemagglutination inhibition test. Specific antibodies against the avian inluenza virus subtype H7 were established in 3 (10%) blood serum samples, in two serum samples from mallards and one sample from a mute swan, using the hemagglutination inhibition test. Specific antibodies against the avian influenza virus type A were not established in any examined bird species using the AGP test. In the population of wild bird species in the Republic of Serbia covered by these investigations, specific antibodies against the avian influenza virus were established only in serum samples of birds of the family Anatidae. Specific antibodies against the avian influenza virus type A established in 3 (6.52%) species of wild birds, and against subtypes H5 and H7 in 2 (4.34%) of the total of 46 examined species. The sensitivity of the cELISA test for the avian influenza virus subtype H5 and the hemagglutination inhibition test for subtype H5 amounted to 100%.
AB  - Infekcije izazvane virusom avijarne influence, su već odavno poznate i prisutne u manjem ili većem obimu, kako u ekstenzivnoj, tako i u intenzivnoj živinarskoj proizvodnji, u mnogim delovima sveta. Epidemiološkim ispitivanjima je utvrđen nesumnjiv značaj populacije divljih ptica u očuvanju i širenju ove infekcije. Avijarna influenca je zoonoza, a virus ima veliki potencijal da izazove visoku smrtnost kod ljudi, posebno njegovi podtipovi H5 i H7, tako da u novije vreme izaziva veliku pažnju, kako naučne i stručne, tako i najšire javnosti. Cilj ovog rada je bio da se na nekoliko lokacija u Republici Srbiji uhvati određeni broj divljih ptica, izvrši njihova identifikacija i prikupe uzorci krvnog seruma radi otkrivanja specifičnih antitela protiv virusa avijarne influence. Hvatanje ptica vršeno je na deset lokacija na bezbedan način, kako za same ptice, tako i za osoblje koje ih je hvatalo. Hvatanje ptica obavljano je posebnim za te svrhe proizvedenim mrežama, a u nekim slučajevima i posebnim zamkama (klopkama). Za identifikaciju uhvaćenih divljih ptica korišćene su metode koje su opisane u stručnoj literaturi. Svi nazivi divljih ptica usklađeni su sa važećom srpskom nomenklaturom ptica Evrope. Nakon hvatanja i identifikacije, pticama je uzimana krv iz krilne vene (kod većih ptica) ili iz nožne vene (kod malih ptica). Prilikom uzimanja krvi od divljih ptica poštovani su svi principi asepse i antisepse, kako bi se sprečila svaka mogućnost infekcije. Nakon toga, ptice su vraćane u prirodu, na iste lokacije na kojima su i uhvaćene. Od uzetih uzoraka krvi izdvojeni su serumi koji su ostavljani na - 20ºC i čuvani do konačnog ispitivanja. Identifikovano je 46 vrsta divljih ptica sa ukupno 259 jedinki od kojih je izdvojeno 259 uzoraka krvnog seruma. Za otkrivanje prisustva specifičnih antitela protiv virusa avijarne influence u uzorcima krvnog seruma divljih ptica korišćeni su agar gel precipitacija (AGP), test inhibicije hemaglutinacije (IH) za podtipove H5 i H7, cELISA test sa antigenom A tipa virusa avijarne influence i cELISA sa antigenom podtipa H5 virusa avijarne influence. S obzirom na činjenicu da na teritoriji Republike Srbije živi oko 360 različitih vrsta divljih ptica, broj od 46 identifikovanih vrsta obuhvaćenih ispitivanjem, činio je 12,77% od ukupnog broja prisutnih vrsta ptica u Srbiji, što se smatra dobrim uzorkom. Specifična antitela protiv A tipa virusa avijarne influence ustanovljena su u uzorcima seruma samo 9 od 259 jedinki koje su bile obuhvaćene ispitivanjem primenom cELISA testa. U identifikovanih 46 vrsta divljih ptica 6 je pripadalo pticama koje žive isključivo u vodenim staništima i smatraju se rezervoarom virusa avijarne influence (bela roda, patka gluvara, labud grbac, riđoglava patka, patka dupljašica i liska). Od navedenih vrsta posebnu pažnju privukle su 4 vrste divljih ptica iz reda Anseriformes i familije Anatidae (patka gluvara, labud grbac, riđoglava patka, patka dupljašica) kojima je od ukupno 259 ptica pripadalo 30 jedinki. U 30 uzoraka krvnog seruma navedenih vrsta ptica, specifična antitela protiv A tipa virusa avijarne influence utvrđena su u 9 (30%) uzoraka seruma, primenom cELISA. Specifična antitela protiv podtipa H5 virusa avijarne influence su ustanovljena u 3 uzorka seruma labudova grbaca (jedan uzorak seruma je poticao od labuda grbca koji je prstenovan u Poljskoj) i u jednom uzorku krvnog seruma riđoglave patke, ili ukupno u 4 (13,33%) uzorka seruma, primenom testa inhibicije hemaglutinacije. Specifična antitela protiv podtipa H7 virusa avijarne influence utvrđena su u 3 (10%) uzorka krvnog seruma i to u dva seruma pataka gluvara i u jednom serumu labuda grbca, primenom inhibicije hemaglutinacije. Specifična antitela protiv A tipa virusa avijarne influence nisu ustanovljena ni kod jedne ispitivane vrste ptice, primenom AGP testa. U populaciji divljih vrta ptica u Republici Srbiji obuhvaćenih ovim ispitivanjem, specifična antitela protiv virusa avijarne influence ustanovljena su samo u uzorcima seruma ptica iz familije Anatidae. Specifična antitela protiv A tipa virusa avijarne influence su otkrivena kod 3 (6,52%) vrste divljih ptica, a protiv podtipova H5 i H7 kod 2 (4,34%) od ukupno 46 vrsta koje su ispitivane. Senzitivnost cELISA testa za podtip H5 virusa avijarne influence i testa inhibicije hemaglutinacije za isti podtip iznosila je 100%.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Examination of presence of specific antibodies against avian influenza virus in some species of wild birds
T1  - Ispitivanje prisustva specifičnih antitela protiv virusa avijarne influence kod nekih vrsta divljih ptica
VL  - 59
IS  - 4
SP  - 381
EP  - 403
DO  - 10.2298/AVB0904381S
ER  - 

@article{
author = "Šekler, Milanko and Ašanin, Ružica and Krnjaić, Dejan and Palić, T. and Milić, Nenad and Jovanović, Tanja and Kovačević, Dragana and Plavšić, B. and Stojanović, Dragica and Vidanović, Dejan and Ašanin, N.",
year = "2009",
abstract = "Infections caused by the avian influenza virus have been known for a long time and they are present, to a smaller or greater extent, in both extensive and intensive poultry production in many parts of the world. Epidemiological investigations have established a definite significance of the population of wild birds in maintaining and spreading this infection. Avian influenza is a zoonosis, and the virus has a great potential for causing mortality in humans, in particular its subtypes H5 and H7, which is why it has lately been provoking much attention among scientists and experts, as well as the general public. The objective of the work was to catch a certain number of wild birds in several locations in the Republic of Serbia, to identify them, and to collect samples of their blood serum for the determination of specific antibodies against the avian influenza virus. Birds were caught in ten locations in a manner that was safe for the birds themselves, as well as for the staff that did the catching. The birds were caught in especially produced nets, and in some cases in special traps. The caught wild birds were identified using the methods described in reference literature. All the names of the wild birds were coordinated with the valid Serbian nomenclature of European birds, prepared by prominent ornithologist and bird lover Milan Ružić. Following catching and identification, blood samples were taken from the birds from the wing vein (in bigger birds) or from the leg vein (in smaller birds). In taking blood samples from wild birds, all the principles of asepsis and antisepsis were followed in order to prevent any possibility of infection. After that, the birds were returned to their natural habitat, to the same locations in which they were caught. Serums were isolated from the taken blood samples and they were stored at -20ºC until the final examinations. A total of 46 species of wild birds were identified among a total of 259 birds from which 259 samples of blood serum were isolated. The following were used for the detection of the presence of specific antibodies against the avian influenza virus in blood serum samples of wild birds: agar gel precipitation (AGP), the hemagglutination inhibition test (HI) for subtypes H5 and H7, the cELISA test with antigen for the A type avian infleunza virus, and the cELISA test with antigen for subtype H5 of the avian influenza virus. Due to the fact that about 360 different species of wild birds live in the Republic of Serbia, the number of 46 identified species covered by these investigations account for 12.77% of the total number of bird species present in Serbia, which is considered a good sample. Specific antibodies against the A type avian influenza virus were established in serum samples of only 9 of the 259 birds covered by examinations using the cELISA test. Of the 46 identified wild bird species, 6 belonged to birds that live exclusively in water habitats and are considered a reservoir of the avian influenza virus (white stork, mallard, mute swan, common pochard, common goldeneye, and Eurasian coot). Among the listed species, particular attention was drawn to 4 species of wild birds of the order Anseriformes and the family Anatidae (mallard, mute swan, common pochard, common goldeneye) of which there were 30 birds among the total of 259 examined. In the 30 blood serum samples of the listed bird species, specific antibodies against the A type avian influenza virus were established in 9 (30%) serum samples using cELISA. Specific antibodies against the avian influenza virus subtype H5 were established in 3 serum samples of mute swans (one serum sample originated from a mute swan which was tagged in Poland) and in one blood serum sample of a common pochard, or a total of 4 (13.33%) serum samples, using the hemagglutination inhibition test. Specific antibodies against the avian inluenza virus subtype H7 were established in 3 (10%) blood serum samples, in two serum samples from mallards and one sample from a mute swan, using the hemagglutination inhibition test. Specific antibodies against the avian influenza virus type A were not established in any examined bird species using the AGP test. In the population of wild bird species in the Republic of Serbia covered by these investigations, specific antibodies against the avian influenza virus were established only in serum samples of birds of the family Anatidae. Specific antibodies against the avian influenza virus type A established in 3 (6.52%) species of wild birds, and against subtypes H5 and H7 in 2 (4.34%) of the total of 46 examined species. The sensitivity of the cELISA test for the avian influenza virus subtype H5 and the hemagglutination inhibition test for subtype H5 amounted to 100%., Infekcije izazvane virusom avijarne influence, su već odavno poznate i prisutne u manjem ili većem obimu, kako u ekstenzivnoj, tako i u intenzivnoj živinarskoj proizvodnji, u mnogim delovima sveta. Epidemiološkim ispitivanjima je utvrđen nesumnjiv značaj populacije divljih ptica u očuvanju i širenju ove infekcije. Avijarna influenca je zoonoza, a virus ima veliki potencijal da izazove visoku smrtnost kod ljudi, posebno njegovi podtipovi H5 i H7, tako da u novije vreme izaziva veliku pažnju, kako naučne i stručne, tako i najšire javnosti. Cilj ovog rada je bio da se na nekoliko lokacija u Republici Srbiji uhvati određeni broj divljih ptica, izvrši njihova identifikacija i prikupe uzorci krvnog seruma radi otkrivanja specifičnih antitela protiv virusa avijarne influence. Hvatanje ptica vršeno je na deset lokacija na bezbedan način, kako za same ptice, tako i za osoblje koje ih je hvatalo. Hvatanje ptica obavljano je posebnim za te svrhe proizvedenim mrežama, a u nekim slučajevima i posebnim zamkama (klopkama). Za identifikaciju uhvaćenih divljih ptica korišćene su metode koje su opisane u stručnoj literaturi. Svi nazivi divljih ptica usklađeni su sa važećom srpskom nomenklaturom ptica Evrope. Nakon hvatanja i identifikacije, pticama je uzimana krv iz krilne vene (kod većih ptica) ili iz nožne vene (kod malih ptica). Prilikom uzimanja krvi od divljih ptica poštovani su svi principi asepse i antisepse, kako bi se sprečila svaka mogućnost infekcije. Nakon toga, ptice su vraćane u prirodu, na iste lokacije na kojima su i uhvaćene. Od uzetih uzoraka krvi izdvojeni su serumi koji su ostavljani na - 20ºC i čuvani do konačnog ispitivanja. Identifikovano je 46 vrsta divljih ptica sa ukupno 259 jedinki od kojih je izdvojeno 259 uzoraka krvnog seruma. Za otkrivanje prisustva specifičnih antitela protiv virusa avijarne influence u uzorcima krvnog seruma divljih ptica korišćeni su agar gel precipitacija (AGP), test inhibicije hemaglutinacije (IH) za podtipove H5 i H7, cELISA test sa antigenom A tipa virusa avijarne influence i cELISA sa antigenom podtipa H5 virusa avijarne influence. S obzirom na činjenicu da na teritoriji Republike Srbije živi oko 360 različitih vrsta divljih ptica, broj od 46 identifikovanih vrsta obuhvaćenih ispitivanjem, činio je 12,77% od ukupnog broja prisutnih vrsta ptica u Srbiji, što se smatra dobrim uzorkom. Specifična antitela protiv A tipa virusa avijarne influence ustanovljena su u uzorcima seruma samo 9 od 259 jedinki koje su bile obuhvaćene ispitivanjem primenom cELISA testa. U identifikovanih 46 vrsta divljih ptica 6 je pripadalo pticama koje žive isključivo u vodenim staništima i smatraju se rezervoarom virusa avijarne influence (bela roda, patka gluvara, labud grbac, riđoglava patka, patka dupljašica i liska). Od navedenih vrsta posebnu pažnju privukle su 4 vrste divljih ptica iz reda Anseriformes i familije Anatidae (patka gluvara, labud grbac, riđoglava patka, patka dupljašica) kojima je od ukupno 259 ptica pripadalo 30 jedinki. U 30 uzoraka krvnog seruma navedenih vrsta ptica, specifična antitela protiv A tipa virusa avijarne influence utvrđena su u 9 (30%) uzoraka seruma, primenom cELISA. Specifična antitela protiv podtipa H5 virusa avijarne influence su ustanovljena u 3 uzorka seruma labudova grbaca (jedan uzorak seruma je poticao od labuda grbca koji je prstenovan u Poljskoj) i u jednom uzorku krvnog seruma riđoglave patke, ili ukupno u 4 (13,33%) uzorka seruma, primenom testa inhibicije hemaglutinacije. Specifična antitela protiv podtipa H7 virusa avijarne influence utvrđena su u 3 (10%) uzorka krvnog seruma i to u dva seruma pataka gluvara i u jednom serumu labuda grbca, primenom inhibicije hemaglutinacije. Specifična antitela protiv A tipa virusa avijarne influence nisu ustanovljena ni kod jedne ispitivane vrste ptice, primenom AGP testa. U populaciji divljih vrta ptica u Republici Srbiji obuhvaćenih ovim ispitivanjem, specifična antitela protiv virusa avijarne influence ustanovljena su samo u uzorcima seruma ptica iz familije Anatidae. Specifična antitela protiv A tipa virusa avijarne influence su otkrivena kod 3 (6,52%) vrste divljih ptica, a protiv podtipova H5 i H7 kod 2 (4,34%) od ukupno 46 vrsta koje su ispitivane. Senzitivnost cELISA testa za podtip H5 virusa avijarne influence i testa inhibicije hemaglutinacije za isti podtip iznosila je 100%.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Examination of presence of specific antibodies against avian influenza virus in some species of wild birds, Ispitivanje prisustva specifičnih antitela protiv virusa avijarne influence kod nekih vrsta divljih ptica",
volume = "59",
number = "4",
pages = "381-403",
doi = "10.2298/AVB0904381S"
}

Šekler, M., Ašanin, R., Krnjaić, D., Palić, T., Milić, N., Jovanović, T., Kovačević, D., Plavšić, B., Stojanović, D., Vidanović, D.,& Ašanin, N.. (2009). Examination of presence of specific antibodies against avian influenza virus in some species of wild birds. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(4), 381-403.
https://doi.org/10.2298/AVB0904381S

Šekler M, Ašanin R, Krnjaić D, Palić T, Milić N, Jovanović T, Kovačević D, Plavšić B, Stojanović D, Vidanović D, Ašanin N. Examination of presence of specific antibodies against avian influenza virus in some species of wild birds. in Acta Veterinaria-Beograd. 2009;59(4):381-403.
doi:10.2298/AVB0904381S .

Šekler, Milanko, Ašanin, Ružica, Krnjaić, Dejan, Palić, T., Milić, Nenad, Jovanović, Tanja, Kovačević, Dragana, Plavšić, B., Stojanović, Dragica, Vidanović, Dejan, Ašanin, N., "Examination of presence of specific antibodies against avian influenza virus in some species of wild birds" in Acta Veterinaria-Beograd, 59, no. 4 (2009):381-403,
https://doi.org/10.2298/AVB0904381S . .