TY  - JOUR
AU  - Vasić, A.
AU  - Oșlobanu, L.E.
AU  - Marinov, M.
AU  - Crivei, L.A.
AU  - Rățoi, I.A.
AU  - Aniță, A.
AU  - Aniță, D.
AU  - Doroșencu, A.
AU  - Alexe, V.
AU  - Raileanu, Cristian
AU  - Simeunović, Predrag
AU  - Raileanu, Cristian
AU  - Falcuța, E.
AU  - Prioteasa, F.L.
AU  - Bojkovski, Jovan
AU  - Pavlović, I.
AU  - Mathis, A.
AU  - Tews, B.A.
AU  - Savuta, Gheorghe
AU  - Veronesi, E.
AU  - Silaghi, Cornelia
AU  - Cimpan, A.
AU  - Ciucă, L.
AU  - Cojkić, A.
AU  - Drašković, Vladimir
AU  - Đurić, M.
AU  - Glavinić, Uroš
AU  - Ivanescu, M.L.
AU  - Kavran, M.
AU  - Lupu, A.
AU  - Mindru, Raluca
AU  - Porea, Daniela
AU  - Radanović, Oliver
AU  - Ristanić, Marko
AU  - Roman, Constantin
AU  - Stanišić, Ljubodrag
AU  - Zdravković, Nemanja
AU  - Vaselek, S.
AU  - SCOPES, AMSAR training group
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1720
AB  - West Nile virus (WNV) is a zoonotic flavivirus whose transmission cycle in nature includes wild birds as amplifying hosts and ornithophilic mosquito vectors. Bridge vectors can transmit WNV to mammal species potentially causing West Nile Fever. Wild bird migration is a mode of WNV introduction into new areas. The Danube Delta Biosphere Reserve (DDBR) is a major stopover of wild birds migrating between Europe and Africa. The aim of this study was to investigate the presence of WNV in the DDBR during the 2016 transmission season in wild birds and mosquitoes. Blood from 68 wild birds (nine different species) trapped at four different locations was analyzed by competitive ELISA and Virus Neutralization Test (VNT), revealing positive results in 8/68 (11.8%) of the wild birds by ELISA of which six samples (three from juvenile birds) were confirmed seropositive by VNT. Mosquitoes (n = 6523, 5 genera) were trapped with CDC Mini Light traps at two locations and in one location resting mosquitoes were caught. The presence of WNV RNA was tested in 134 pools by reverse transcription quantitative PCR (RT-qPCR). None of the pools was positive for WNV-specific RNA. Based on the obtained results, WNV was circulating in the DDBR during 2016.
PB  - MDPI AG
T2  - Tropical Medicine and Infectious Disease
T1  - Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016
VL  - 4
IS  - 3
SP  - 116
DO  - 10.3390/tropicalmed4030116
ER  - 

@article{
author = "Vasić, A. and Oșlobanu, L.E. and Marinov, M. and Crivei, L.A. and Rățoi, I.A. and Aniță, A. and Aniță, D. and Doroșencu, A. and Alexe, V. and Raileanu, Cristian and Simeunović, Predrag and Raileanu, Cristian and Falcuța, E. and Prioteasa, F.L. and Bojkovski, Jovan and Pavlović, I. and Mathis, A. and Tews, B.A. and Savuta, Gheorghe and Veronesi, E. and Silaghi, Cornelia and Cimpan, A. and Ciucă, L. and Cojkić, A. and Drašković, Vladimir and Đurić, M. and Glavinić, Uroš and Ivanescu, M.L. and Kavran, M. and Lupu, A. and Mindru, Raluca and Porea, Daniela and Radanović, Oliver and Ristanić, Marko and Roman, Constantin and Stanišić, Ljubodrag and Zdravković, Nemanja and Vaselek, S. and SCOPES, AMSAR training group",
year = "2019",
abstract = "West Nile virus (WNV) is a zoonotic flavivirus whose transmission cycle in nature includes wild birds as amplifying hosts and ornithophilic mosquito vectors. Bridge vectors can transmit WNV to mammal species potentially causing West Nile Fever. Wild bird migration is a mode of WNV introduction into new areas. The Danube Delta Biosphere Reserve (DDBR) is a major stopover of wild birds migrating between Europe and Africa. The aim of this study was to investigate the presence of WNV in the DDBR during the 2016 transmission season in wild birds and mosquitoes. Blood from 68 wild birds (nine different species) trapped at four different locations was analyzed by competitive ELISA and Virus Neutralization Test (VNT), revealing positive results in 8/68 (11.8%) of the wild birds by ELISA of which six samples (three from juvenile birds) were confirmed seropositive by VNT. Mosquitoes (n = 6523, 5 genera) were trapped with CDC Mini Light traps at two locations and in one location resting mosquitoes were caught. The presence of WNV RNA was tested in 134 pools by reverse transcription quantitative PCR (RT-qPCR). None of the pools was positive for WNV-specific RNA. Based on the obtained results, WNV was circulating in the DDBR during 2016.",
publisher = "MDPI AG",
journal = "Tropical Medicine and Infectious Disease",
title = "Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016",
volume = "4",
number = "3",
pages = "116",
doi = "10.3390/tropicalmed4030116"
}

Vasić, A., Oșlobanu, L.E., Marinov, M., Crivei, L.A., Rățoi, I.A., Aniță, A., Aniță, D., Doroșencu, A., Alexe, V., Raileanu, C., Simeunović, P., Raileanu, C., Falcuța, E., Prioteasa, F.L., Bojkovski, J., Pavlović, I., Mathis, A., Tews, B.A., Savuta, G., Veronesi, E., Silaghi, C., Cimpan, A., Ciucă, L., Cojkić, A., Drašković, V., Đurić, M., Glavinić, U., Ivanescu, M.L., Kavran, M., Lupu, A., Mindru, R., Porea, D., Radanović, O., Ristanić, M., Roman, C., Stanišić, L., Zdravković, N., Vaselek, S.,& SCOPES, A. t. g.. (2019). Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016. in Tropical Medicine and Infectious Disease
MDPI AG., 4(3), 116.
https://doi.org/10.3390/tropicalmed4030116

Vasić A, Oșlobanu L, Marinov M, Crivei L, Rățoi I, Aniță A, Aniță D, Doroșencu A, Alexe V, Raileanu C, Simeunović P, Raileanu C, Falcuța E, Prioteasa F, Bojkovski J, Pavlović I, Mathis A, Tews B, Savuta G, Veronesi E, Silaghi C, Cimpan A, Ciucă L, Cojkić A, Drašković V, Đurić M, Glavinić U, Ivanescu M, Kavran M, Lupu A, Mindru R, Porea D, Radanović O, Ristanić M, Roman C, Stanišić L, Zdravković N, Vaselek S, SCOPES ATG. Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016. in Tropical Medicine and Infectious Disease. 2019;4(3):116.
doi:10.3390/tropicalmed4030116 .

Vasić, A., Oșlobanu, L.E., Marinov, M., Crivei, L.A., Rățoi, I.A., Aniță, A., Aniță, D., Doroșencu, A., Alexe, V., Raileanu, Cristian, Simeunović, Predrag, Raileanu, Cristian, Falcuța, E., Prioteasa, F.L., Bojkovski, Jovan, Pavlović, I., Mathis, A., Tews, B.A., Savuta, Gheorghe, Veronesi, E., Silaghi, Cornelia, Cimpan, A., Ciucă, L., Cojkić, A., Drašković, Vladimir, Đurić, M., Glavinić, Uroš, Ivanescu, M.L., Kavran, M., Lupu, A., Mindru, Raluca, Porea, Daniela, Radanović, Oliver, Ristanić, Marko, Roman, Constantin, Stanišić, Ljubodrag, Zdravković, Nemanja, Vaselek, S., SCOPES, AMSAR training group, "Evidence of West Nile virus (WNV) circulation in wild birds and WNV RNA negativity in mosquitoes of the Danube Delta Biosphere Reserve, Romania, 2016" in Tropical Medicine and Infectious Disease, 4, no. 3 (2019):116,
https://doi.org/10.3390/tropicalmed4030116 . .

TY  - JOUR
AU  - Veronesi, E.
AU  - Paslaru, A.
AU  - Silaghi, Cornelia
AU  - Tobler, K.
AU  - Glavinić, Uroš
AU  - Torgerson, P.
AU  - Mathis, A.
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1597
AB  - West Nile virus (WNV) is continuously spreading in Eastern and Southern Europe. However, the extent of vector competence of Aedes japonicus (Theobald, 1901) is controversial. In this work, we elucidated the dynamics of virus growth in this invasive mosquito species. Females of Ae. japonicus were reared from eggs collected in the field in Switzerland and fed on bovine blood spiked with two WNV lineage 1 strains (FIN, Italy; NY99, USA). Fully engorged females were incubated for 14 days under a fluctuating temperature regime of 24 ± 7 °C (average 24 °C), 45–90% relative humidity, which is realistic for a Central European mid-summer day. Infection, dissemination, and transmission rates were assessed from individual mosquitoes by analyzing the abdomen, legs and wings, and saliva for the presence of viral RNA. Saliva was also investigated for the presence of infectious virus particles. Overall, 302 females were exposed to WNV strain FIN and 293 to strain NY99. A higher infection rate was observed for NY99 (57.4%) compared to FIN (30.4%) (p = 0.003). There was no statistical evidence that the dissemination rate (viral RNA in legs and wings) was different between females infected with FIN (57.1%) compared to NY99 (35.5%) (p = 0.16). Viral RNA load of FIN compared to NY99 was significantly higher in the hemocoel (p = 0.031) of exposed females but not at other sites (legs and wings, saliva). This is the first study describing the vector competence parameters for two WNV strains in a European population of Ae. japonicus. The high dissemination and transmission rates for WNV under a realistic temperature regime in Ae. japonicus together with recent findings on its opportunistic feeding behavior (mammals and birds) indicate its potential role in WNV transmission in Central Europe where it is highly abundant.
PB  - Springer Verlag
T2  - Parasitology Research
T1  - Experimental evaluation of infection, dissemination, and transmission rates for two West Nile virus strains in European Aedes japonicus under a fluctuating temperature regime
VL  - 117
IS  - 6
SP  - 1925
EP  - 1932
DO  - 10.1007/s00436-018-5886-7
ER  - 

@article{
author = "Veronesi, E. and Paslaru, A. and Silaghi, Cornelia and Tobler, K. and Glavinić, Uroš and Torgerson, P. and Mathis, A.",
year = "2018",
abstract = "West Nile virus (WNV) is continuously spreading in Eastern and Southern Europe. However, the extent of vector competence of Aedes japonicus (Theobald, 1901) is controversial. In this work, we elucidated the dynamics of virus growth in this invasive mosquito species. Females of Ae. japonicus were reared from eggs collected in the field in Switzerland and fed on bovine blood spiked with two WNV lineage 1 strains (FIN, Italy; NY99, USA). Fully engorged females were incubated for 14 days under a fluctuating temperature regime of 24 ± 7 °C (average 24 °C), 45–90% relative humidity, which is realistic for a Central European mid-summer day. Infection, dissemination, and transmission rates were assessed from individual mosquitoes by analyzing the abdomen, legs and wings, and saliva for the presence of viral RNA. Saliva was also investigated for the presence of infectious virus particles. Overall, 302 females were exposed to WNV strain FIN and 293 to strain NY99. A higher infection rate was observed for NY99 (57.4%) compared to FIN (30.4%) (p = 0.003). There was no statistical evidence that the dissemination rate (viral RNA in legs and wings) was different between females infected with FIN (57.1%) compared to NY99 (35.5%) (p = 0.16). Viral RNA load of FIN compared to NY99 was significantly higher in the hemocoel (p = 0.031) of exposed females but not at other sites (legs and wings, saliva). This is the first study describing the vector competence parameters for two WNV strains in a European population of Ae. japonicus. The high dissemination and transmission rates for WNV under a realistic temperature regime in Ae. japonicus together with recent findings on its opportunistic feeding behavior (mammals and birds) indicate its potential role in WNV transmission in Central Europe where it is highly abundant.",
publisher = "Springer Verlag",
journal = "Parasitology Research",
title = "Experimental evaluation of infection, dissemination, and transmission rates for two West Nile virus strains in European Aedes japonicus under a fluctuating temperature regime",
volume = "117",
number = "6",
pages = "1925-1932",
doi = "10.1007/s00436-018-5886-7"
}

Veronesi, E., Paslaru, A., Silaghi, C., Tobler, K., Glavinić, U., Torgerson, P.,& Mathis, A.. (2018). Experimental evaluation of infection, dissemination, and transmission rates for two West Nile virus strains in European Aedes japonicus under a fluctuating temperature regime. in Parasitology Research
Springer Verlag., 117(6), 1925-1932.
https://doi.org/10.1007/s00436-018-5886-7

Veronesi E, Paslaru A, Silaghi C, Tobler K, Glavinić U, Torgerson P, Mathis A. Experimental evaluation of infection, dissemination, and transmission rates for two West Nile virus strains in European Aedes japonicus under a fluctuating temperature regime. in Parasitology Research. 2018;117(6):1925-1932.
doi:10.1007/s00436-018-5886-7 .

Veronesi, E., Paslaru, A., Silaghi, Cornelia, Tobler, K., Glavinić, Uroš, Torgerson, P., Mathis, A., "Experimental evaluation of infection, dissemination, and transmission rates for two West Nile virus strains in European Aedes japonicus under a fluctuating temperature regime" in Parasitology Research, 117, no. 6 (2018):1925-1932,
https://doi.org/10.1007/s00436-018-5886-7 . .