TY  - JOUR
AU  - Vučević, Danijela B.
AU  - Borozan, Sunčica
AU  - Radenković, Ana S.
AU  - Radosavljević, Milica I.
AU  - Mladenović, Dušan R.
AU  - Jorgačević, Bojan Z.
AU  - Samardžić, Janko M.
AU  - Vesković, Milena N.
AU  - Vukičević, Dušan V.
AU  - Radosavljević, Tatjana S.
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1861
AB  - Galectin-3 (Gal-3) has increasingly been recognized as a modulator of inflammation, oxidative/nitrosative stress, fibrogenesis, and tissue remodeling. The objective of the current pilot study was to investigate the influence of Gal-3 on cardiac and renal antioxidant capacity using biochemical and histopathological examinations. Two groups (n=7 each) of male mice were tested: 1. control (CON) group (wild type of C57BL/6 mice) and 2. GAL-3-/- group (galectin-3-/- knockout mice). After overnight fasting, mice were sacrificed by exsanguination in ketamine (100mg/kg intraperitoneally). Then, cardiac and renal tissue samples were taken to determine the parameters of oxidative/nitrosative stress and antioxidant capacity. The levels of malondialdehyde and nitrites+nitrates was not significantly different in the GAL-3-/- group vs. the CON group. The total superoxide dismutase activity in the renal tissue of the GAL-3-/- mice was significantly lower compared to the CON group. Cardiac and renal catalase and glutathione S-transferase activity was significantly reduced in the GAL-3-/- group vs. the CON group, respectively. A significant decrease in glutathione level was also registered in hearts of the GAL-3-/- group vs. the CON group. Our findings indicate that Gal-3 deficiency does not lead to lipid peroxidation and nitrosative stress in cardiac and renal tissue in mice. However, the lack of this beta-galactoside-binding lectin does reduce antioxidant capacity in both of the investigated tissues.
PB  - Institute of Systematics and Evolution of Animals
T2  - Folia Biologica (Poland)
T1  - Galectin-3 deficiency reduces cardiac and renal antioxidant capacity in mice
VL  - 68
IS  - 2
SP  - 73
EP  - 80
DO  - 10.3409/fb_68-2.09
ER  - 

@article{
author = "Vučević, Danijela B. and Borozan, Sunčica and Radenković, Ana S. and Radosavljević, Milica I. and Mladenović, Dušan R. and Jorgačević, Bojan Z. and Samardžić, Janko M. and Vesković, Milena N. and Vukičević, Dušan V. and Radosavljević, Tatjana S.",
year = "2020",
abstract = "Galectin-3 (Gal-3) has increasingly been recognized as a modulator of inflammation, oxidative/nitrosative stress, fibrogenesis, and tissue remodeling. The objective of the current pilot study was to investigate the influence of Gal-3 on cardiac and renal antioxidant capacity using biochemical and histopathological examinations. Two groups (n=7 each) of male mice were tested: 1. control (CON) group (wild type of C57BL/6 mice) and 2. GAL-3-/- group (galectin-3-/- knockout mice). After overnight fasting, mice were sacrificed by exsanguination in ketamine (100mg/kg intraperitoneally). Then, cardiac and renal tissue samples were taken to determine the parameters of oxidative/nitrosative stress and antioxidant capacity. The levels of malondialdehyde and nitrites+nitrates was not significantly different in the GAL-3-/- group vs. the CON group. The total superoxide dismutase activity in the renal tissue of the GAL-3-/- mice was significantly lower compared to the CON group. Cardiac and renal catalase and glutathione S-transferase activity was significantly reduced in the GAL-3-/- group vs. the CON group, respectively. A significant decrease in glutathione level was also registered in hearts of the GAL-3-/- group vs. the CON group. Our findings indicate that Gal-3 deficiency does not lead to lipid peroxidation and nitrosative stress in cardiac and renal tissue in mice. However, the lack of this beta-galactoside-binding lectin does reduce antioxidant capacity in both of the investigated tissues.",
publisher = "Institute of Systematics and Evolution of Animals",
journal = "Folia Biologica (Poland)",
title = "Galectin-3 deficiency reduces cardiac and renal antioxidant capacity in mice",
volume = "68",
number = "2",
pages = "73-80",
doi = "10.3409/fb_68-2.09"
}

Vučević, D. B., Borozan, S., Radenković, A. S., Radosavljević, M. I., Mladenović, D. R., Jorgačević, B. Z., Samardžić, J. M., Vesković, M. N., Vukičević, D. V.,& Radosavljević, T. S.. (2020). Galectin-3 deficiency reduces cardiac and renal antioxidant capacity in mice. in Folia Biologica (Poland)
Institute of Systematics and Evolution of Animals., 68(2), 73-80.
https://doi.org/10.3409/fb_68-2.09

Vučević DB, Borozan S, Radenković AS, Radosavljević MI, Mladenović DR, Jorgačević BZ, Samardžić JM, Vesković MN, Vukičević DV, Radosavljević TS. Galectin-3 deficiency reduces cardiac and renal antioxidant capacity in mice. in Folia Biologica (Poland). 2020;68(2):73-80.
doi:10.3409/fb_68-2.09 .

Vučević, Danijela B., Borozan, Sunčica, Radenković, Ana S., Radosavljević, Milica I., Mladenović, Dušan R., Jorgačević, Bojan Z., Samardžić, Janko M., Vesković, Milena N., Vukičević, Dušan V., Radosavljević, Tatjana S., "Galectin-3 deficiency reduces cardiac and renal antioxidant capacity in mice" in Folia Biologica (Poland), 68, no. 2 (2020):73-80,
https://doi.org/10.3409/fb_68-2.09 . .