TY  - JOUR
AU  - Dmitrić, Marko
AU  - Vidanović, Dejan
AU  - Matović, Kazimir
AU  - Šekler, Milanko
AU  - Sarić, Ljubiša
AU  - Arsić, Milos
AU  - Karabasil, Nedjeljko
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1641
AB  - The microbiological standard method for the detection of Salmonella spp. is time-consuming, and consequently there is a need for an alternative rapid methodology for its detection. In this study, two open-formula real-time PCR methods for detecting the inv A and ttr gene Salmonella spp. have been successfully optimized and in-house validated. Different DNA extraction procedures were used (boiling, Chelex resin and standard I - Biorad). The relative sensitivity and false positive ratio for the alternative methods were 100% and 0.0%, respectively. The relative trueness was in range from 96.8% to 99.2%. No false negative results were detected. The lowest C-t values were obtained using the protocol for detecting the ttr gene after DNA extraction by the Chelex. The results were compared in a large number of food and environmental samples to those of the SRPS EN ISO 6579:2008 standard method and commercial kit (IQ check((R))Salmonella II kit, Bio-Rad, USA). Practical applicationsThe whole procedure of real-time PCR methods in this study was approximately 20 hr, in contrast to 4-5 days of analysis time for the standard method (SRPS EN ISO 6579:2008). The real-time PCR method also provides a high level of sensitivity and specificity with a low risk of cross-contamination. The implementation of the method for routine analysis will help improve safety in the food production chain, by providing results compatible with the ISO standard, but more rapidly.
PB  - Wiley, Hoboken
T2  - Journal of Food Processing and Preservation
T1  - In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food
VL  - 42
IS  - 2
SP  - e13455
DO  - 10.1111/jfpp.13455
ER  - 

@article{
author = "Dmitrić, Marko and Vidanović, Dejan and Matović, Kazimir and Šekler, Milanko and Sarić, Ljubiša and Arsić, Milos and Karabasil, Nedjeljko",
year = "2018",
abstract = "The microbiological standard method for the detection of Salmonella spp. is time-consuming, and consequently there is a need for an alternative rapid methodology for its detection. In this study, two open-formula real-time PCR methods for detecting the inv A and ttr gene Salmonella spp. have been successfully optimized and in-house validated. Different DNA extraction procedures were used (boiling, Chelex resin and standard I - Biorad). The relative sensitivity and false positive ratio for the alternative methods were 100% and 0.0%, respectively. The relative trueness was in range from 96.8% to 99.2%. No false negative results were detected. The lowest C-t values were obtained using the protocol for detecting the ttr gene after DNA extraction by the Chelex. The results were compared in a large number of food and environmental samples to those of the SRPS EN ISO 6579:2008 standard method and commercial kit (IQ check((R))Salmonella II kit, Bio-Rad, USA). Practical applicationsThe whole procedure of real-time PCR methods in this study was approximately 20 hr, in contrast to 4-5 days of analysis time for the standard method (SRPS EN ISO 6579:2008). The real-time PCR method also provides a high level of sensitivity and specificity with a low risk of cross-contamination. The implementation of the method for routine analysis will help improve safety in the food production chain, by providing results compatible with the ISO standard, but more rapidly.",
publisher = "Wiley, Hoboken",
journal = "Journal of Food Processing and Preservation",
title = "In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food",
volume = "42",
number = "2",
pages = "e13455",
doi = "10.1111/jfpp.13455"
}

Dmitrić, M., Vidanović, D., Matović, K., Šekler, M., Sarić, L., Arsić, M.,& Karabasil, N.. (2018). In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food. in Journal of Food Processing and Preservation
Wiley, Hoboken., 42(2), e13455.
https://doi.org/10.1111/jfpp.13455

Dmitrić M, Vidanović D, Matović K, Šekler M, Sarić L, Arsić M, Karabasil N. In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food. in Journal of Food Processing and Preservation. 2018;42(2):e13455.
doi:10.1111/jfpp.13455 .

Dmitrić, Marko, Vidanović, Dejan, Matović, Kazimir, Šekler, Milanko, Sarić, Ljubiša, Arsić, Milos, Karabasil, Nedjeljko, "In-house validation of real-time PCR methods for detecting the INV A and TTR genes of Salmonella spp. in food" in Journal of Food Processing and Preservation, 42, no. 2 (2018):e13455,
https://doi.org/10.1111/jfpp.13455 . .