@article{
author = "Vujinović, Slobodan and Graber, Hans Ulrich and Vićić, Ivan and Vejnović, Branislav and Stevanović, Oliver and Krnjaić, Dejan and Milivojević, Dušan and Katić, Vera",
year = "2023",
abstract = "This study aimed to determine the prevalence of Staphylococcus aureus subclinical mastitis and to genotype the
S. aureus isolates using the 16S–23S rRNA intergenic spacer (RS-PCR) method. In addition, the genes responsible
for adherence, biofilm formation, host evasion, tissue necrosis, methicillin resistance, and enterotoxin production
of S. aureus were investigated. The overall prevalence of S. aureus subclinical mastitis in lactating cows was 5.4%
(95% confidence interval, CI=4.7–6.1%). An increased risk of S. aureus intramammary infection was observed on
small family farms (odds ratio, OR=4.2, 95% CI=2.6–6.6, P < 0.001) and medium-sized farms (OR=3.5, 95%
CI=2.2–5.7, P < 0.001). The RS-PCR analysis revealed 44 genotypes and genotype variants, of which 15 new
genotypes and five new variants were detected within small and medium-sized farms. S. aureus isolates of new
genotypes and genotype variants carried the clfA gene responsible for adherence at a lower frequency (64.8%)
and enterotoxin-producing genes sea (20.4%), seb (14.8%) and sec (14.8%) at a higher frequency than the other
known genotypes (P < 0.001), and were confirmed to carry the sej and sep genes. The spa gene was detected in all
S. aureus isolates, whereas none harbored bap, ser, or tsst-1 genes. Methicillin-resistant strains of S. aureus (MRSA)
were also detected, with a higher prevalence (19.2%) on large farms with more than 50 cows (P < 0.001). Using
molecular techniques as diagnostic tools provides a better understanding of intramammary staphylococcal infections’ occurrence, spread, and eradication.",
publisher = "Elsevier",
journal = "Comparative Immunology, Microbiology and Infectious Diseases",
title = "Genotypes and virulence factors in Staphylococcus aureus isolated from dairy cows with subclinical mastitis in Serbia",
volume = "101",
pages = "102056",
doi = "10.1016/j.cimid.2023.102056"
}