TY  - JOUR
AU  - Betić, Nikola
AU  - Karabasil, Nedjeljko
AU  - Djurković-Djaković, Olgica
AU  - Ćirković, Vladimir
AU  - Bobić, Branko
AU  - Branković Lazić, Ivana
AU  - Djordjević, Vesna
AU  - Klun, Ivana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2464
AB  - Consumption of Toxoplasma gondii contaminated pork is a major risk factor for human infection. We thus conducted a cross-sectional survey on the seroprevalence of T. gondii infection in a representative sample of slaughter pigs from throughout Serbia and examined the influence of farm biosecurity-related risk factors on infection. In addition, direct detection of the parasite (by mouse bioassay) or its DNA was performed in the hearts of a subset of seropositive sows. The overall seroprevalence in the sample of 825 pigs as determined by the modified agglutination test (MAT) was 16.5%. Older age and inadequate rodent control were independent infection risk factors for pigs. In a subset of 581 pigs with complete biosecurity-related data, in addition to older age, smallholders’ finishing type farms (as opposed to farrow-to-finish), multispecies farming, and origin from Western and Central and South-Eastern Serbia (vs. the Northern region), all increased the risk of infection, while the absence of disinfection boot-dips in front of each barn and Belgrade district origin (vs. the Northern region) were associated with a 62% and 75% lower risk of infection, respectively. Evidence of viable parasites was obtained in 13 (41.9%) of the 31 bioassayed sow hearts, of which by isolation of brain cysts in seven, by detection of T. gondii DNA in an additional four, and by serology in another two. Recovery of brain cysts mostly (5/7) from sows with a MAT titre of ≥1:100 indicates the risk for consumers. These results highlight the public health risk from pork consumption and point to mandatory use of professional rodent control services, abstaining from multispecies farming, keeping disinfection boot-dips clean and freshly refilled, as well as strict implementation of zoo-hygienic measures on smallholders’ farms as specific farm biosecurity measures needed for its reduction.
PB  - MDPI
T2  - Microorganisms
T1  - Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures
VL  - 10
IS  - 5
SP  - 1069
DO  - 10.3390/microorganisms10051069
ER  - 

@article{
author = "Betić, Nikola and Karabasil, Nedjeljko and Djurković-Djaković, Olgica and Ćirković, Vladimir and Bobić, Branko and Branković Lazić, Ivana and Djordjević, Vesna and Klun, Ivana",
year = "2022",
abstract = "Consumption of Toxoplasma gondii contaminated pork is a major risk factor for human infection. We thus conducted a cross-sectional survey on the seroprevalence of T. gondii infection in a representative sample of slaughter pigs from throughout Serbia and examined the influence of farm biosecurity-related risk factors on infection. In addition, direct detection of the parasite (by mouse bioassay) or its DNA was performed in the hearts of a subset of seropositive sows. The overall seroprevalence in the sample of 825 pigs as determined by the modified agglutination test (MAT) was 16.5%. Older age and inadequate rodent control were independent infection risk factors for pigs. In a subset of 581 pigs with complete biosecurity-related data, in addition to older age, smallholders’ finishing type farms (as opposed to farrow-to-finish), multispecies farming, and origin from Western and Central and South-Eastern Serbia (vs. the Northern region), all increased the risk of infection, while the absence of disinfection boot-dips in front of each barn and Belgrade district origin (vs. the Northern region) were associated with a 62% and 75% lower risk of infection, respectively. Evidence of viable parasites was obtained in 13 (41.9%) of the 31 bioassayed sow hearts, of which by isolation of brain cysts in seven, by detection of T. gondii DNA in an additional four, and by serology in another two. Recovery of brain cysts mostly (5/7) from sows with a MAT titre of ≥1:100 indicates the risk for consumers. These results highlight the public health risk from pork consumption and point to mandatory use of professional rodent control services, abstaining from multispecies farming, keeping disinfection boot-dips clean and freshly refilled, as well as strict implementation of zoo-hygienic measures on smallholders’ farms as specific farm biosecurity measures needed for its reduction.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures",
volume = "10",
number = "5",
pages = "1069",
doi = "10.3390/microorganisms10051069"
}

Betić, N., Karabasil, N., Djurković-Djaković, O., Ćirković, V., Bobić, B., Branković Lazić, I., Djordjević, V.,& Klun, I.. (2022). Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures. in Microorganisms
MDPI., 10(5), 1069.
https://doi.org/10.3390/microorganisms10051069

Betić N, Karabasil N, Djurković-Djaković O, Ćirković V, Bobić B, Branković Lazić I, Djordjević V, Klun I. Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures. in Microorganisms. 2022;10(5):1069.
doi:10.3390/microorganisms10051069 .

Betić, Nikola, Karabasil, Nedjeljko, Djurković-Djaković, Olgica, Ćirković, Vladimir, Bobić, Branko, Branković Lazić, Ivana, Djordjević, Vesna, Klun, Ivana, "Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures" in Microorganisms, 10, no. 5 (2022):1069,
https://doi.org/10.3390/microorganisms10051069 . .

TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Dimitrijević, Sanda
AU  - Katić-Radivojević, Sofija
AU  - Klun, Ivana
AU  - Bobić, Branko
AU  - Đurković-Đaković, Olgica
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/540
AB  - To identify areas of risk for canine-related zoonoses in Serbia, the aim of this Study was to provide baseline knowledge about intestinal parasites in 15 1 dogs (65 household pets, 75 stray and I I military working dogs) from Belgrade. The following parasites, with their respective prevalences, were detected: Giardia duodenalis (14.6%), Ancylostomatidae (24.5%), Toxocara canis (30.5%), Trichuris vulpis (47.0%) and Taenia-type helminths (6.6%). Of all examined dogs, 75.5% (114/151) were found to harbour at least one parasite species. Of these, mixed infections with Up to four species per dog occurred in 44.7% (51/114). Infections with all detected species were significantly higher (p < 0.05) in military working (100%) and stray dogs (93.3%) versus household pets (50.8%). Among all parasites, agents with zoonotic potential including Giardia, Ancylostomatidae and Toxocara were detected in 58.3% (88/151) of all examined dogs with a significant difference (p < 0.05) among the subgroups (100%, 62.7% and 46.2% for military working dogs, stray dogs and household pets, respectively). The high prevalence of zoonotic parasites registered in the dog population from a highly urban area in south-eastern Europe indicates a potential risk to human health. Thus, veterinarians should play an important role in helping to prevent or minimise zoonotic transmission.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - High prevalence of intestinal zoonotic parasites in dogs from Belgrade, Serbia - Short communication
VL  - 56
IS  - 3
SP  - 335
EP  - 340
DO  - 10.1556/AVet.56.2008.3.7
ER  - 

@article{
author = "Nikolić, Aleksandra and Dimitrijević, Sanda and Katić-Radivojević, Sofija and Klun, Ivana and Bobić, Branko and Đurković-Đaković, Olgica",
year = "2008",
abstract = "To identify areas of risk for canine-related zoonoses in Serbia, the aim of this Study was to provide baseline knowledge about intestinal parasites in 15 1 dogs (65 household pets, 75 stray and I I military working dogs) from Belgrade. The following parasites, with their respective prevalences, were detected: Giardia duodenalis (14.6%), Ancylostomatidae (24.5%), Toxocara canis (30.5%), Trichuris vulpis (47.0%) and Taenia-type helminths (6.6%). Of all examined dogs, 75.5% (114/151) were found to harbour at least one parasite species. Of these, mixed infections with Up to four species per dog occurred in 44.7% (51/114). Infections with all detected species were significantly higher (p < 0.05) in military working (100%) and stray dogs (93.3%) versus household pets (50.8%). Among all parasites, agents with zoonotic potential including Giardia, Ancylostomatidae and Toxocara were detected in 58.3% (88/151) of all examined dogs with a significant difference (p < 0.05) among the subgroups (100%, 62.7% and 46.2% for military working dogs, stray dogs and household pets, respectively). The high prevalence of zoonotic parasites registered in the dog population from a highly urban area in south-eastern Europe indicates a potential risk to human health. Thus, veterinarians should play an important role in helping to prevent or minimise zoonotic transmission.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "High prevalence of intestinal zoonotic parasites in dogs from Belgrade, Serbia - Short communication",
volume = "56",
number = "3",
pages = "335-340",
doi = "10.1556/AVet.56.2008.3.7"
}

Nikolić, A., Dimitrijević, S., Katić-Radivojević, S., Klun, I., Bobić, B.,& Đurković-Đaković, O.. (2008). High prevalence of intestinal zoonotic parasites in dogs from Belgrade, Serbia - Short communication. in Acta Veterinaria Hungarica
Akademiai Kiado Zrt, Budapest., 56(3), 335-340.
https://doi.org/10.1556/AVet.56.2008.3.7

Nikolić A, Dimitrijević S, Katić-Radivojević S, Klun I, Bobić B, Đurković-Đaković O. High prevalence of intestinal zoonotic parasites in dogs from Belgrade, Serbia - Short communication. in Acta Veterinaria Hungarica. 2008;56(3):335-340.
doi:10.1556/AVet.56.2008.3.7 .

Nikolić, Aleksandra, Dimitrijević, Sanda, Katić-Radivojević, Sofija, Klun, Ivana, Bobić, Branko, Đurković-Đaković, Olgica, "High prevalence of intestinal zoonotic parasites in dogs from Belgrade, Serbia - Short communication" in Acta Veterinaria Hungarica, 56, no. 3 (2008):335-340,
https://doi.org/10.1556/AVet.56.2008.3.7 . .

TY  - JOUR
AU  - Nikolić, Aleksandra
AU  - Dimitrijević, Sanda
AU  - Đurković-Đaković, Olgica
AU  - Bobić, Branko
AU  - Maksimović-Mihajlović, Olga
PY  - 2002
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/212
AB  - Giardia infection is an ongoing problem in Serbia, since 8,0% of the children in the Belgrade area are infected. In the light of the zoonotic potential of Giardia infection, a total of 248 companion animals (167 dogs and 81 cats) were examined for giardiasis in the same urban area. The overall rates of infection in dogs and cats were 14.4% (24/167) and 22.2 % (18/81), respectively. Significantly (p lt 0.05, p lt 0.01) higher infection rates were found in stray (18.7%) and farm (36.4%) dogs than in household pets (7.4%). Furthermore, the rate of infection was significantly (p lt 0.01) higher in domestic cats than in household dogs (22.2%vs. 7.4%). The owners and all 16 of their family members of five out of the six household pet dogs excreting Giardia were Giardia-negative, while both the owner of one dog and the only other family member were Giardia-positive. The data demonstrate that potential animal reservoirs (dogs and cats) exist in urban Serbia and underline the possibility of transmission between dog and man in close contact, thus having important implications for the epidemiology of giardiasis in urban areas.
AB  - Kako nalaz 8% inficirane dece u Beogradu pokazuje da Giardia predstavlja važan zdravstveni problem u Srbiji, a postoje indicije da je đardijoza zoonoza, u cilju provere pretpostavki o mogućoj ulozi pasa i mačaka urbanih sredina kao potencijalnih rezervoara infekcije ljudi, u studiji je ispitano 248 ovih životinja (167 pasa i 81 mačaka) sa područja grada Beograda. Ustanovljena je prevalenca infekcije od 14,4% (24/167) kod pasa i 22,2% (18/81) kod mačaka. Značajno su češće (p lt 0,05, p lt 0,01) bill inficirani kako psi lutalice (18,6%) i psi savojnefarme (36,4%), tako i kućne mačke (14,4%) u odnosu na pse kućne ljubimce (7,4%). Kod šest pasa kućnih ljubimaca koji su bili Giardia-pozitivni, pet vlasnika i 16 članova njihovih porodica su bili Giardia-negativni, dok je u jednim slučaju ustanovljena istovremena infekcija psa, vlasnika i preostalog člana te porodice. Rezultati ove studije doprinose boljem razumevanju epidemiologije ove patogene protpzoe na našim prostorima, dokazujući postojanje značajnih izvora Giardia infekcije kod pasa i mačaka u urbanim uslovima, koji zbog bliskih kontakta kućnih ljubimca i ljudi, mogu biti odgovorni za humanu infekciju.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Giardiasis in dogs and cats in the Belgrade area
T1  - Đardijoza pasa i mačaka u Beogradu
VL  - 52
IS  - 1
SP  - 43
EP  - 48
DO  - 10.2298/AVB0201043N
ER  - 

@article{
author = "Nikolić, Aleksandra and Dimitrijević, Sanda and Đurković-Đaković, Olgica and Bobić, Branko and Maksimović-Mihajlović, Olga",
year = "2002",
abstract = "Giardia infection is an ongoing problem in Serbia, since 8,0% of the children in the Belgrade area are infected. In the light of the zoonotic potential of Giardia infection, a total of 248 companion animals (167 dogs and 81 cats) were examined for giardiasis in the same urban area. The overall rates of infection in dogs and cats were 14.4% (24/167) and 22.2 % (18/81), respectively. Significantly (p lt 0.05, p lt 0.01) higher infection rates were found in stray (18.7%) and farm (36.4%) dogs than in household pets (7.4%). Furthermore, the rate of infection was significantly (p lt 0.01) higher in domestic cats than in household dogs (22.2%vs. 7.4%). The owners and all 16 of their family members of five out of the six household pet dogs excreting Giardia were Giardia-negative, while both the owner of one dog and the only other family member were Giardia-positive. The data demonstrate that potential animal reservoirs (dogs and cats) exist in urban Serbia and underline the possibility of transmission between dog and man in close contact, thus having important implications for the epidemiology of giardiasis in urban areas., Kako nalaz 8% inficirane dece u Beogradu pokazuje da Giardia predstavlja važan zdravstveni problem u Srbiji, a postoje indicije da je đardijoza zoonoza, u cilju provere pretpostavki o mogućoj ulozi pasa i mačaka urbanih sredina kao potencijalnih rezervoara infekcije ljudi, u studiji je ispitano 248 ovih životinja (167 pasa i 81 mačaka) sa područja grada Beograda. Ustanovljena je prevalenca infekcije od 14,4% (24/167) kod pasa i 22,2% (18/81) kod mačaka. Značajno su češće (p lt 0,05, p lt 0,01) bill inficirani kako psi lutalice (18,6%) i psi savojnefarme (36,4%), tako i kućne mačke (14,4%) u odnosu na pse kućne ljubimce (7,4%). Kod šest pasa kućnih ljubimaca koji su bili Giardia-pozitivni, pet vlasnika i 16 članova njihovih porodica su bili Giardia-negativni, dok je u jednim slučaju ustanovljena istovremena infekcija psa, vlasnika i preostalog člana te porodice. Rezultati ove studije doprinose boljem razumevanju epidemiologije ove patogene protpzoe na našim prostorima, dokazujući postojanje značajnih izvora Giardia infekcije kod pasa i mačaka u urbanim uslovima, koji zbog bliskih kontakta kućnih ljubimca i ljudi, mogu biti odgovorni za humanu infekciju.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Giardiasis in dogs and cats in the Belgrade area, Đardijoza pasa i mačaka u Beogradu",
volume = "52",
number = "1",
pages = "43-48",
doi = "10.2298/AVB0201043N"
}

Nikolić, A., Dimitrijević, S., Đurković-Đaković, O., Bobić, B.,& Maksimović-Mihajlović, O.. (2002). Giardiasis in dogs and cats in the Belgrade area. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 52(1), 43-48.
https://doi.org/10.2298/AVB0201043N

Nikolić A, Dimitrijević S, Đurković-Đaković O, Bobić B, Maksimović-Mihajlović O. Giardiasis in dogs and cats in the Belgrade area. in Acta Veterinaria-Beograd. 2002;52(1):43-48.
doi:10.2298/AVB0201043N .

Nikolić, Aleksandra, Dimitrijević, Sanda, Đurković-Đaković, Olgica, Bobić, Branko, Maksimović-Mihajlović, Olga, "Giardiasis in dogs and cats in the Belgrade area" in Acta Veterinaria-Beograd, 52, no. 1 (2002):43-48,
https://doi.org/10.2298/AVB0201043N . .

TY  - JOUR
AU  - Vuković, D.
AU  - Đurković-Đaković, Olgica
AU  - Kovačević, Sanja
AU  - Bobić, Branko
AU  - Nikolíc, A.
AU  - Todorović, V.
AU  - Babić, D.
PY  - 1997
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/84
AB  - Objective: To characterize the antitoxoplasma activity of clindamycin in a murine model of acute toxoplasmosis. Methods: Rates of survival and mean survival times of Swiss Webster mice infected intraperitoneally with 106-102 tachyzoites of the RH strain of Toxoplasma gondii treated with clindamycin or sulfamethoxazole (positive control) or untreated (negative control) were compared. Survivors were submitted to examination of untreated brain tissue preparations, intraperitoneal and peroral subinoculations of brain tissue homogenates into fresh mice, and to pathohistology, including immunohistochemistry, of brain and lungs. Results: The effect of clindamycin treatment (400 mg/kg/day) on infected Swiss Webster mice was inoculum size dependent, ranging from no survivals in animals infected with 106 parasites, to 100% survivals with an inoculum of 102. Treatment initiated 24 h before and at time of infection prolonged mean survival times comparably to sulfamethoxazole, and significantly when compared to untreated controls. In contrast, treatment initiated 48 h postinfection with an inoculum of 106 did not postpone death. In the clindamycin-treated survivors, there was no biological or histologic evidence for the persistence of toxoplasma. Conclusions: The results obtained show that at an appropriate parasite dose/drug dose ratio, clindamycin is strongly toxoplasmacidal in a murine model of acute toxoplasmosis.
PB  - Blackwell Publishing Ltd
T2  - Clinical Microbiology and Infection
T1  - Effect of clindamycin in a model of acute murine toxoplasmosis
VL  - 3
IS  - 1
SP  - 89
EP  - 94
DO  - 10.1111/j.1469-0691.1997.tb00256.x
ER  - 

@article{
author = "Vuković, D. and Đurković-Đaković, Olgica and Kovačević, Sanja and Bobić, Branko and Nikolíc, A. and Todorović, V. and Babić, D.",
year = "1997",
abstract = "Objective: To characterize the antitoxoplasma activity of clindamycin in a murine model of acute toxoplasmosis. Methods: Rates of survival and mean survival times of Swiss Webster mice infected intraperitoneally with 106-102 tachyzoites of the RH strain of Toxoplasma gondii treated with clindamycin or sulfamethoxazole (positive control) or untreated (negative control) were compared. Survivors were submitted to examination of untreated brain tissue preparations, intraperitoneal and peroral subinoculations of brain tissue homogenates into fresh mice, and to pathohistology, including immunohistochemistry, of brain and lungs. Results: The effect of clindamycin treatment (400 mg/kg/day) on infected Swiss Webster mice was inoculum size dependent, ranging from no survivals in animals infected with 106 parasites, to 100% survivals with an inoculum of 102. Treatment initiated 24 h before and at time of infection prolonged mean survival times comparably to sulfamethoxazole, and significantly when compared to untreated controls. In contrast, treatment initiated 48 h postinfection with an inoculum of 106 did not postpone death. In the clindamycin-treated survivors, there was no biological or histologic evidence for the persistence of toxoplasma. Conclusions: The results obtained show that at an appropriate parasite dose/drug dose ratio, clindamycin is strongly toxoplasmacidal in a murine model of acute toxoplasmosis.",
publisher = "Blackwell Publishing Ltd",
journal = "Clinical Microbiology and Infection",
title = "Effect of clindamycin in a model of acute murine toxoplasmosis",
volume = "3",
number = "1",
pages = "89-94",
doi = "10.1111/j.1469-0691.1997.tb00256.x"
}

Vuković, D., Đurković-Đaković, O., Kovačević, S., Bobić, B., Nikolíc, A., Todorović, V.,& Babić, D.. (1997). Effect of clindamycin in a model of acute murine toxoplasmosis. in Clinical Microbiology and Infection
Blackwell Publishing Ltd., 3(1), 89-94.
https://doi.org/10.1111/j.1469-0691.1997.tb00256.x

Vuković D, Đurković-Đaković O, Kovačević S, Bobić B, Nikolíc A, Todorović V, Babić D. Effect of clindamycin in a model of acute murine toxoplasmosis. in Clinical Microbiology and Infection. 1997;3(1):89-94.
doi:10.1111/j.1469-0691.1997.tb00256.x .

Vuković, D., Đurković-Đaković, Olgica, Kovačević, Sanja, Bobić, Branko, Nikolíc, A., Todorović, V., Babić, D., "Effect of clindamycin in a model of acute murine toxoplasmosis" in Clinical Microbiology and Infection, 3, no. 1 (1997):89-94,
https://doi.org/10.1111/j.1469-0691.1997.tb00256.x . .