TY  - JOUR
AU  - Marković, Aleksandra
AU  - Tubić Vukajlović, Jovana
AU  - Grujičić, Darko
AU  - Radović Jakovljević, Marina
AU  - Stanković, Milan
AU  - Djordjević, Katarina
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Milošević-Djordjević, Olivera
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2244
AB  - The study was designed to evaluate antigenotoxic effect of methanol Teucrium arduini and Teucrium flavum extracts against mitomycin C (MMC)-induced chromosome and DNA damage in vitro. Cytokinesis-block micronucleus (CBMN) and comet assays were used to investigate effect of plant extracts in different concentrations (125, 250, 500 and 1000 µg/mL) on human peripheral blood lymphocytes (PBLs). The obtained results showed that the all tested concentrations of T. arduini and the highest concentration of T. flavum significantly reduced the MMC-induced micronucleus (MN) frequency in comparison to positive control (only MMC). There were significantly negative correlations between the extracts concentrations and MN frequencies (Pearson, r = −0.905, p = 0.0001 for T. arduini; r = −0.861, p = 0.0001 for T. flavum). The extracts of both plants further lowered the MMC-decreased nuclear division index (NDI) in a dose dependent-manner (Pearson, r = −0.837, p = 0.001 for T. arduini; r = −0.598, p = 0.040 for T. flavum), but significantly only in the highest concentration (1000 µg/mL). Comet assay showed that extracts reduced MMC-increased genetic damage index (GDI), significantly in the concentrations of 500 and 1000 μg/mL, in comparison with positive control. Based on our results, it can be concluded that methanol T. arduini and T. flavum extracts possess protective proapoptotic and antigenotoxic effect which is indication of their medicinal relevance and use in treatment.
AB  - The study was designed to evaluate antigenotoxic effect of methanol Teucrium arduini and Teucrium flavum extracts against mitomycin C (MMC)-induced chromosome and DNA damage in vitro. Cytokinesis-block micronucleus (CBMN) and comet assays were used to investigate effect of plant extracts in different concentrations (125, 250, 500 and 1000 µg/mL) on human peripheral blood lymphocytes (PBLs). The obtained results showed that the all tested concentrations of T. arduini and the highest concentration of T. flavum significantly reduced the MMC-induced micronucleus (MN) frequency in comparison to positive control (only MMC). There were significantly negative correlations between the extracts concentrations and MN frequencies (Pearson, r = −0.905, p = 0.0001 for T. arduini; r = −0.861, p = 0.0001 for T. flavum). The extracts of both plants further lowered the MMC-decreased nuclear division index (NDI) in a dose dependent-manner (Pearson, r = −0.837, p = 0.001 for T. arduini; r = −0.598, p = 0.040 for T. flavum), but significantly only in the highest concentration (1000 µg/mL). Comet assay showed that extracts reduced MMC-increased genetic damage index (GDI), significantly in the concentrations of 500 and 1000 μg/mL, in comparison with positive control. Based on our results, it can be concluded that methanol T. arduini and T. flavum extracts possess protective proapoptotic and antigenotoxic effect which is indication of their medicinal relevance and use in treatment.
PB  - Taylor and Francis
T2  - Drug and Chemical Toxicology
T1  - Methanol extracts of Teucrium arduini L. and Teucrium flavum L. induce protective effect against mitomycin C in human lymphocytes in vitro
VL  - 45
IS  - 2
SP  - 940
EP  - 945
DO  - 10.1080/01480545.2020.1802477
ER  - 

@article{
author = "Marković, Aleksandra and Tubić Vukajlović, Jovana and Grujičić, Darko and Radović Jakovljević, Marina and Stanković, Milan and Djordjević, Katarina and Đelić, Ninoslav and Radaković, Milena and Milošević-Djordjević, Olivera",
year = "2022",
abstract = "The study was designed to evaluate antigenotoxic effect of methanol Teucrium arduini and Teucrium flavum extracts against mitomycin C (MMC)-induced chromosome and DNA damage in vitro. Cytokinesis-block micronucleus (CBMN) and comet assays were used to investigate effect of plant extracts in different concentrations (125, 250, 500 and 1000 µg/mL) on human peripheral blood lymphocytes (PBLs). The obtained results showed that the all tested concentrations of T. arduini and the highest concentration of T. flavum significantly reduced the MMC-induced micronucleus (MN) frequency in comparison to positive control (only MMC). There were significantly negative correlations between the extracts concentrations and MN frequencies (Pearson, r = −0.905, p = 0.0001 for T. arduini; r = −0.861, p = 0.0001 for T. flavum). The extracts of both plants further lowered the MMC-decreased nuclear division index (NDI) in a dose dependent-manner (Pearson, r = −0.837, p = 0.001 for T. arduini; r = −0.598, p = 0.040 for T. flavum), but significantly only in the highest concentration (1000 µg/mL). Comet assay showed that extracts reduced MMC-increased genetic damage index (GDI), significantly in the concentrations of 500 and 1000 μg/mL, in comparison with positive control. Based on our results, it can be concluded that methanol T. arduini and T. flavum extracts possess protective proapoptotic and antigenotoxic effect which is indication of their medicinal relevance and use in treatment., The study was designed to evaluate antigenotoxic effect of methanol Teucrium arduini and Teucrium flavum extracts against mitomycin C (MMC)-induced chromosome and DNA damage in vitro. Cytokinesis-block micronucleus (CBMN) and comet assays were used to investigate effect of plant extracts in different concentrations (125, 250, 500 and 1000 µg/mL) on human peripheral blood lymphocytes (PBLs). The obtained results showed that the all tested concentrations of T. arduini and the highest concentration of T. flavum significantly reduced the MMC-induced micronucleus (MN) frequency in comparison to positive control (only MMC). There were significantly negative correlations between the extracts concentrations and MN frequencies (Pearson, r = −0.905, p = 0.0001 for T. arduini; r = −0.861, p = 0.0001 for T. flavum). The extracts of both plants further lowered the MMC-decreased nuclear division index (NDI) in a dose dependent-manner (Pearson, r = −0.837, p = 0.001 for T. arduini; r = −0.598, p = 0.040 for T. flavum), but significantly only in the highest concentration (1000 µg/mL). Comet assay showed that extracts reduced MMC-increased genetic damage index (GDI), significantly in the concentrations of 500 and 1000 μg/mL, in comparison with positive control. Based on our results, it can be concluded that methanol T. arduini and T. flavum extracts possess protective proapoptotic and antigenotoxic effect which is indication of their medicinal relevance and use in treatment.",
publisher = "Taylor and Francis",
journal = "Drug and Chemical Toxicology",
title = "Methanol extracts of Teucrium arduini L. and Teucrium flavum L. induce protective effect against mitomycin C in human lymphocytes in vitro",
volume = "45",
number = "2",
pages = "940-945",
doi = "10.1080/01480545.2020.1802477"
}

Marković, A., Tubić Vukajlović, J., Grujičić, D., Radović Jakovljević, M., Stanković, M., Djordjević, K., Đelić, N., Radaković, M.,& Milošević-Djordjević, O.. (2022). Methanol extracts of Teucrium arduini L. and Teucrium flavum L. induce protective effect against mitomycin C in human lymphocytes in vitro. in Drug and Chemical Toxicology
Taylor and Francis., 45(2), 940-945.
https://doi.org/10.1080/01480545.2020.1802477

Marković A, Tubić Vukajlović J, Grujičić D, Radović Jakovljević M, Stanković M, Djordjević K, Đelić N, Radaković M, Milošević-Djordjević O. Methanol extracts of Teucrium arduini L. and Teucrium flavum L. induce protective effect against mitomycin C in human lymphocytes in vitro. in Drug and Chemical Toxicology. 2022;45(2):940-945.
doi:10.1080/01480545.2020.1802477 .

Marković, Aleksandra, Tubić Vukajlović, Jovana, Grujičić, Darko, Radović Jakovljević, Marina, Stanković, Milan, Djordjević, Katarina, Đelić, Ninoslav, Radaković, Milena, Milošević-Djordjević, Olivera, "Methanol extracts of Teucrium arduini L. and Teucrium flavum L. induce protective effect against mitomycin C in human lymphocytes in vitro" in Drug and Chemical Toxicology, 45, no. 2 (2022):940-945,
https://doi.org/10.1080/01480545.2020.1802477 . .

TY  - JOUR
AU  - Tubić Vukajlović, Jovana
AU  - Kosanić, Marijana
AU  - Ranković, Branislav
AU  - Stanojković, Tatjana
AU  - Marković, Aleksandra
AU  - Grujičić, Darko
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Milošević-Djordjević, Olivera
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2318
AB  - Bioactivity of acetone extract of Leccinum scabrum was evaluated. Antioxidant activity was evaluated by free radical scavenging activity, reducing power and determination of total phenolic content. The microdilution plate method was used to detect antimicrobial activity. Cytotoxic activity was tested by MTT assay. Genotoxic potential was evaluated by comet assay. Results showed a stronger free radical scavenging activity, but the reducing power was less pronounced. The total content of phenols in the extract was 14.91 µg PE/mg. The minimum inhibitory concentration related to the tested microorganisms fluctuated in the range 0.039-10 mg/mL. The most sensitive was Bacillus cereus. A strong cytotoxic activity was revealed with IC50 values ranging between 73.64 and 200.00 µg/mL, among which HeLa was the most sensitive. Results showed that the extract was not genotoxic and it decreased the H2O2-induced GDI values in comparison to the positive control. L. scabrum possesses bioactivities such as antioxidant, antimicrobial, cytotoxic, no genotoxic and protective against H2O2, and in the highest tested concentration it showed a synergistic activity.
AB  - Studiul prezintă evaluarea activității biologice a extractului în acetonă obținut din Leccinum scabrum. Activitatea antioxidantă a fost evaluată prin determinarea capacității de chelatare a radicalilor liberi, a capacității reducătoare și a conținutului total în fenoli. Activitatea antimicrobiană a fost testată prin metoda microdiluției, activitatea citotoxică prin metoda MTT, iar potențialul genotoxic a fost determinat prin calcularea indexului de deteriorare genetică. Conținutul total de fenoli a fost 14,91 μg PE/mg. Concentrația minimă inhibitorie a variat în intervalul 0,039 - 10 mg/mL. Activitatea citotoxică pronunțată a fost corelată cu valorile IC50 în domeniul 73,64 - 200,00 μg/mL. Rezultatele au arătat că extractul nu este genotoxic și au scăzut valorile indexului de deteriorare genetică-indus de H2O2 , în comparație cu controlul pozitiv. L. scabrum posedă activități antioxidantă, antimicrobiană, citotoxică șı protectoare împotriva H2O2.
PB  - Societatea de Științe Farmaceutice din România
T2  - Farmacia
T1  - Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum
VL  - 69
IS  - 5
SP  - 974
EP  - 979
DO  - 10.31925/FARMACIA.2021.5.22
ER  - 

@article{
author = "Tubić Vukajlović, Jovana and Kosanić, Marijana and Ranković, Branislav and Stanojković, Tatjana and Marković, Aleksandra and Grujičić, Darko and Đelić, Ninoslav and Radaković, Milena and Milošević-Djordjević, Olivera",
year = "2021",
abstract = "Bioactivity of acetone extract of Leccinum scabrum was evaluated. Antioxidant activity was evaluated by free radical scavenging activity, reducing power and determination of total phenolic content. The microdilution plate method was used to detect antimicrobial activity. Cytotoxic activity was tested by MTT assay. Genotoxic potential was evaluated by comet assay. Results showed a stronger free radical scavenging activity, but the reducing power was less pronounced. The total content of phenols in the extract was 14.91 µg PE/mg. The minimum inhibitory concentration related to the tested microorganisms fluctuated in the range 0.039-10 mg/mL. The most sensitive was Bacillus cereus. A strong cytotoxic activity was revealed with IC50 values ranging between 73.64 and 200.00 µg/mL, among which HeLa was the most sensitive. Results showed that the extract was not genotoxic and it decreased the H2O2-induced GDI values in comparison to the positive control. L. scabrum possesses bioactivities such as antioxidant, antimicrobial, cytotoxic, no genotoxic and protective against H2O2, and in the highest tested concentration it showed a synergistic activity., Studiul prezintă evaluarea activității biologice a extractului în acetonă obținut din Leccinum scabrum. Activitatea antioxidantă a fost evaluată prin determinarea capacității de chelatare a radicalilor liberi, a capacității reducătoare și a conținutului total în fenoli. Activitatea antimicrobiană a fost testată prin metoda microdiluției, activitatea citotoxică prin metoda MTT, iar potențialul genotoxic a fost determinat prin calcularea indexului de deteriorare genetică. Conținutul total de fenoli a fost 14,91 μg PE/mg. Concentrația minimă inhibitorie a variat în intervalul 0,039 - 10 mg/mL. Activitatea citotoxică pronunțată a fost corelată cu valorile IC50 în domeniul 73,64 - 200,00 μg/mL. Rezultatele au arătat că extractul nu este genotoxic și au scăzut valorile indexului de deteriorare genetică-indus de H2O2 , în comparație cu controlul pozitiv. L. scabrum posedă activități antioxidantă, antimicrobiană, citotoxică șı protectoare împotriva H2O2.",
publisher = "Societatea de Științe Farmaceutice din România",
journal = "Farmacia",
title = "Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum",
volume = "69",
number = "5",
pages = "974-979",
doi = "10.31925/FARMACIA.2021.5.22"
}

Tubić Vukajlović, J., Kosanić, M., Ranković, B., Stanojković, T., Marković, A., Grujičić, D., Đelić, N., Radaković, M.,& Milošević-Djordjević, O.. (2021). Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum. in Farmacia
Societatea de Științe Farmaceutice din România., 69(5), 974-979.
https://doi.org/10.31925/FARMACIA.2021.5.22

Tubić Vukajlović J, Kosanić M, Ranković B, Stanojković T, Marković A, Grujičić D, Đelić N, Radaković M, Milošević-Djordjević O. Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum. in Farmacia. 2021;69(5):974-979.
doi:10.31925/FARMACIA.2021.5.22 .

Tubić Vukajlović, Jovana, Kosanić, Marijana, Ranković, Branislav, Stanojković, Tatjana, Marković, Aleksandra, Grujičić, Darko, Đelić, Ninoslav, Radaković, Milena, Milošević-Djordjević, Olivera, "Evaluation of biological activities of acetone extract of the mushroom leccinum scabrum" in Farmacia, 69, no. 5 (2021):974-979,
https://doi.org/10.31925/FARMACIA.2021.5.22 . .

TY  - CONF
AU  - Tubić, Jovana
AU  - Marković, Aleksandra
AU  - Ranković, Branislav
AU  - Kosanić, Marijana
AU  - Đelić, Ninoslav
AU  - Radaković, Milena
AU  - Grujičić, Darko
AU  - Milošević-Đorđević, Olivera
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2610
PB  - Beograd : Srpsko biološko društvo
C3  - Drugi kongres biologa Srbije, Kladovo, 25–30.09.2018.
T1  - Komet test u detekciji antigenotoksičnog efekta etanolnog ekstrakta gljive Lactarius piperatus
SP  - 135
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2610
ER  - 

@conference{
author = "Tubić, Jovana and Marković, Aleksandra and Ranković, Branislav and Kosanić, Marijana and Đelić, Ninoslav and Radaković, Milena and Grujičić, Darko and Milošević-Đorđević, Olivera",
year = "2018",
publisher = "Beograd : Srpsko biološko društvo",
journal = "Drugi kongres biologa Srbije, Kladovo, 25–30.09.2018.",
title = "Komet test u detekciji antigenotoksičnog efekta etanolnog ekstrakta gljive Lactarius piperatus",
pages = "135-135",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2610"
}

Tubić, J., Marković, A., Ranković, B., Kosanić, M., Đelić, N., Radaković, M., Grujičić, D.,& Milošević-Đorđević, O.. (2018). Komet test u detekciji antigenotoksičnog efekta etanolnog ekstrakta gljive Lactarius piperatus. in Drugi kongres biologa Srbije, Kladovo, 25–30.09.2018.
Beograd : Srpsko biološko društvo., 135-135.
https://hdl.handle.net/21.15107/rcub_veterinar_2610

Tubić J, Marković A, Ranković B, Kosanić M, Đelić N, Radaković M, Grujičić D, Milošević-Đorđević O. Komet test u detekciji antigenotoksičnog efekta etanolnog ekstrakta gljive Lactarius piperatus. in Drugi kongres biologa Srbije, Kladovo, 25–30.09.2018.. 2018;:135-135.
https://hdl.handle.net/21.15107/rcub_veterinar_2610 .

Tubić, Jovana, Marković, Aleksandra, Ranković, Branislav, Kosanić, Marijana, Đelić, Ninoslav, Radaković, Milena, Grujičić, Darko, Milošević-Đorđević, Olivera, "Komet test u detekciji antigenotoksičnog efekta etanolnog ekstrakta gljive Lactarius piperatus" in Drugi kongres biologa Srbije, Kladovo, 25–30.09.2018. (2018):135-135,
https://hdl.handle.net/21.15107/rcub_veterinar_2610 .