TY  - JOUR
AU  - Kiskároly, Ferenc
AU  - Morić, Ivana
AU  - Dokić, Lidija
AU  - Vasiljević, Branka
AU  - Senerović, Lidija
AU  - Mišić, Dušan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1480
AB  - The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specific primers to identify Salmonella enterica species and its serotypes that are most frequently isolated from poultry samples in Vojvodina. Using the slide agglutination test, 64 and 33 out of 107 Salmonella isolates were identified as S. Infantis and S. Enteritidis, respectively, while ten isolates were identified as eight different Salmonella serovars. Using the same isolates, presence of 993-bp (bcfC gene), 636-bp (steB gene) and 293-bp (sdf locus) amplicons in multiplex PCR unambiguously identified 31 isolates as S. Enteritidis. Two isolates identified as Enteritidis in slide agglutination test were not identified as such in PCR-based approach since they both were missing 293bp long PCR product. Thirty-nine isolates produced a 727-bp amplicon in the specific simplex PCR, and thus were identified as S. Infantis. The greatest discrepancy in comparison to the results of conventional serotyping has been observed in the case of S. Infantis, since 25 more isolates were noted as S. Infantis by conventional serotyping. Seven isolates, with unexpected PCR profiles stayed unidentified by molecular typing, although they were serotyped as S. Typhimurium (1) and S. Infantis (6). S. Gallinarum serovar has to be additionally confirmed, since it shares the same PCR profile with S. Livingstone. Clearly, PCR-based identification has to be thoroughly checked, verified and adapted if it is to be applied as the routine identification protocol.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Development of PCR-based identification of salmonella enterica serovars
VL  - 67
IS  - 2
SP  - 277
EP  - 290
DO  - 10.1515/acve-2017-0022
ER  - 

@article{
author = "Kiskároly, Ferenc and Morić, Ivana and Dokić, Lidija and Vasiljević, Branka and Senerović, Lidija and Mišić, Dušan",
year = "2017",
abstract = "The aim of the study was to evaluate and adapt the PCR-based protocol that utilizes the developed serotype-specific primers to identify Salmonella enterica species and its serotypes that are most frequently isolated from poultry samples in Vojvodina. Using the slide agglutination test, 64 and 33 out of 107 Salmonella isolates were identified as S. Infantis and S. Enteritidis, respectively, while ten isolates were identified as eight different Salmonella serovars. Using the same isolates, presence of 993-bp (bcfC gene), 636-bp (steB gene) and 293-bp (sdf locus) amplicons in multiplex PCR unambiguously identified 31 isolates as S. Enteritidis. Two isolates identified as Enteritidis in slide agglutination test were not identified as such in PCR-based approach since they both were missing 293bp long PCR product. Thirty-nine isolates produced a 727-bp amplicon in the specific simplex PCR, and thus were identified as S. Infantis. The greatest discrepancy in comparison to the results of conventional serotyping has been observed in the case of S. Infantis, since 25 more isolates were noted as S. Infantis by conventional serotyping. Seven isolates, with unexpected PCR profiles stayed unidentified by molecular typing, although they were serotyped as S. Typhimurium (1) and S. Infantis (6). S. Gallinarum serovar has to be additionally confirmed, since it shares the same PCR profile with S. Livingstone. Clearly, PCR-based identification has to be thoroughly checked, verified and adapted if it is to be applied as the routine identification protocol.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Development of PCR-based identification of salmonella enterica serovars",
volume = "67",
number = "2",
pages = "277-290",
doi = "10.1515/acve-2017-0022"
}

Kiskároly, F., Morić, I., Dokić, L., Vasiljević, B., Senerović, L.,& Mišić, D.. (2017). Development of PCR-based identification of salmonella enterica serovars. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(2), 277-290.
https://doi.org/10.1515/acve-2017-0022

Kiskároly F, Morić I, Dokić L, Vasiljević B, Senerović L, Mišić D. Development of PCR-based identification of salmonella enterica serovars. in Acta Veterinaria-Beograd. 2017;67(2):277-290.
doi:10.1515/acve-2017-0022 .

Kiskároly, Ferenc, Morić, Ivana, Dokić, Lidija, Vasiljević, Branka, Senerović, Lidija, Mišić, Dušan, "Development of PCR-based identification of salmonella enterica serovars" in Acta Veterinaria-Beograd, 67, no. 2 (2017):277-290,
https://doi.org/10.1515/acve-2017-0022 . .