TY  - JOUR
AU  - Ledina, Tijana
AU  - Mohar-Lorbeg, Petra
AU  - Golob, Majda
AU  - Đorđević, Jasna
AU  - Bogović-Matijasić, Bojana
AU  - Bulajić, Snežana
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1635
AB  - The aim of this study was to investigate the presence of tetracycline resistance in lactobacilli isolated from traditional Serbian white brined raw milk cheeses (Homolje, Sjenica, Zlatar). Isolation of presumptive lactobacilli was initially performed using MRS-S agar without tetracycline, or supplemented with 16 and 64 A mu g/mL of tetracycline. Rep-PCR (GTG)(5) genotyping showed a high diversity of the isolates obtained, as examination of 233 isolates resulted in 156 different Rep-PCR fingerprints. Ninety out of 156 (57.69%) of the strains, representatives with different (GTG)(5) fingerprints, were identified by MALDI-TOF MS as lactobacilli, while 66 out of 156 (42.31%) strains were identified as members of other LAB genera. All except one out of 90 Lactobacillus isolates further tested by microdilution method, demonstrated unimodal distribution of tetracycline MIC values which were equal to or lower from the breakpoint MIC values (EFSA in EFSA J 10: 1-10, 2012. Only one Lb. paracasei isolate showed the presence of tet(M) gene, while the other analyzed tet genes [tet(A), tet(B), tet(C) tet(K), tet(L), tet(O) and tet(W)] were not detected in any of the isolates. The results of this study indicates that lactobacilli from traditional Serbian raw milk cheeses do not present considerable tetracycline resistance reservoirs. For final conclusions about the safety of these autochthonous cheeses regarding the possible tetracycline resistance transferability, the assessment of the entire cheese microbiota is needed.
PB  - Springer India, New Delhi
T2  - Journal of Food Science and Technology-Mysore
T1  - Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses
VL  - 55
IS  - 4
SP  - 1426
EP  - 1434
DO  - 10.1007/s13197-018-3057-6
ER  - 

@article{
author = "Ledina, Tijana and Mohar-Lorbeg, Petra and Golob, Majda and Đorđević, Jasna and Bogović-Matijasić, Bojana and Bulajić, Snežana",
year = "2018",
abstract = "The aim of this study was to investigate the presence of tetracycline resistance in lactobacilli isolated from traditional Serbian white brined raw milk cheeses (Homolje, Sjenica, Zlatar). Isolation of presumptive lactobacilli was initially performed using MRS-S agar without tetracycline, or supplemented with 16 and 64 A mu g/mL of tetracycline. Rep-PCR (GTG)(5) genotyping showed a high diversity of the isolates obtained, as examination of 233 isolates resulted in 156 different Rep-PCR fingerprints. Ninety out of 156 (57.69%) of the strains, representatives with different (GTG)(5) fingerprints, were identified by MALDI-TOF MS as lactobacilli, while 66 out of 156 (42.31%) strains were identified as members of other LAB genera. All except one out of 90 Lactobacillus isolates further tested by microdilution method, demonstrated unimodal distribution of tetracycline MIC values which were equal to or lower from the breakpoint MIC values (EFSA in EFSA J 10: 1-10, 2012. Only one Lb. paracasei isolate showed the presence of tet(M) gene, while the other analyzed tet genes [tet(A), tet(B), tet(C) tet(K), tet(L), tet(O) and tet(W)] were not detected in any of the isolates. The results of this study indicates that lactobacilli from traditional Serbian raw milk cheeses do not present considerable tetracycline resistance reservoirs. For final conclusions about the safety of these autochthonous cheeses regarding the possible tetracycline resistance transferability, the assessment of the entire cheese microbiota is needed.",
publisher = "Springer India, New Delhi",
journal = "Journal of Food Science and Technology-Mysore",
title = "Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses",
volume = "55",
number = "4",
pages = "1426-1434",
doi = "10.1007/s13197-018-3057-6"
}

Ledina, T., Mohar-Lorbeg, P., Golob, M., Đorđević, J., Bogović-Matijasić, B.,& Bulajić, S.. (2018). Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses. in Journal of Food Science and Technology-Mysore
Springer India, New Delhi., 55(4), 1426-1434.
https://doi.org/10.1007/s13197-018-3057-6

Ledina T, Mohar-Lorbeg P, Golob M, Đorđević J, Bogović-Matijasić B, Bulajić S. Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses. in Journal of Food Science and Technology-Mysore. 2018;55(4):1426-1434.
doi:10.1007/s13197-018-3057-6 .

Ledina, Tijana, Mohar-Lorbeg, Petra, Golob, Majda, Đorđević, Jasna, Bogović-Matijasić, Bojana, Bulajić, Snežana, "Tetracycline resistance in lactobacilli isolated from Serbian traditional raw milk cheeses" in Journal of Food Science and Technology-Mysore, 55, no. 4 (2018):1426-1434,
https://doi.org/10.1007/s13197-018-3057-6 . .