TY  - JOUR
AU  - Velebit, Branko
AU  - Milojević, Lazar
AU  - Baltić, Tatjana
AU  - Grković, Nevena
AU  - Gummalla, Sanjay
AU  - Velebit, Marina
AU  - Škoko, Ines
AU  - Mojsova, Sandra
AU  - Putnik, Predrag
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2836
AB  - In this study, the effectiveness of cold atmospheric plasma (CAP) in inactivating murine norovirus (MNV/human norovirus surrogate) and hepatitis A virus (HAV) on aerosol-inoculated dark red Willamette raspberries was explored. Pulsed positive corona discharge system fed by synthetic air was used for the production of CAP. Raspberries were treated for 1, 3, 5, 7, and 10 min at 25 W. Application of CAP enabled a 4 log10 infectivity reduction in <5 min for MNV and approximately 10 min for HAV (from starting level of 6.91 and 7.84 log10 PFU/mL, respectively). Viral genome copies reduction of 3.18 log10 for MNV and 4.32 for HAV were found from starting level of 5.76 and 6.47 log10 gc/μL, respectively. CAP treatment did not result in significant degradation of fruit color, an important quality attribute. The study demonstrated CAP as an efficient post-harvest decontamination method to reduce viral load in raspberries without significantly affecting its quality parameters. Industrial relevance: Due to the fast-processing paces required in the raspberry industry, it is difficult to assure the complete microbiological safety of this fruit. Cold atmospheric plasma is a practical, environmentally-friendly, non-thermal tool for the effective reduction of microbial pathogens. The model developed in this study demonstrated that CAP treatment of fresh raspberries not only inactivated hazardous enteric viruses in a short time (10 min) but also unaffected fruit color stability. The simplicity of described CAP design and low-cost inputs (air and electricity) enable the commercial application of inexpensive plasma chambers for continuous surface decontamination of large volumes of raspberries without bringing processing to a standstill.
PB  - Elsevier
T2  - Innovative Food Science and Emerging Technologies
T1  - Efficacy of cold atmospheric plasma for inactivation of viruses on raspberries
VL  - 81
SP  - 103121
DO  - 10.1016/j.ifset.2022.103121
ER  - 

@article{
author = "Velebit, Branko and Milojević, Lazar and Baltić, Tatjana and Grković, Nevena and Gummalla, Sanjay and Velebit, Marina and Škoko, Ines and Mojsova, Sandra and Putnik, Predrag",
year = "2022",
abstract = "In this study, the effectiveness of cold atmospheric plasma (CAP) in inactivating murine norovirus (MNV/human norovirus surrogate) and hepatitis A virus (HAV) on aerosol-inoculated dark red Willamette raspberries was explored. Pulsed positive corona discharge system fed by synthetic air was used for the production of CAP. Raspberries were treated for 1, 3, 5, 7, and 10 min at 25 W. Application of CAP enabled a 4 log10 infectivity reduction in <5 min for MNV and approximately 10 min for HAV (from starting level of 6.91 and 7.84 log10 PFU/mL, respectively). Viral genome copies reduction of 3.18 log10 for MNV and 4.32 for HAV were found from starting level of 5.76 and 6.47 log10 gc/μL, respectively. CAP treatment did not result in significant degradation of fruit color, an important quality attribute. The study demonstrated CAP as an efficient post-harvest decontamination method to reduce viral load in raspberries without significantly affecting its quality parameters. Industrial relevance: Due to the fast-processing paces required in the raspberry industry, it is difficult to assure the complete microbiological safety of this fruit. Cold atmospheric plasma is a practical, environmentally-friendly, non-thermal tool for the effective reduction of microbial pathogens. The model developed in this study demonstrated that CAP treatment of fresh raspberries not only inactivated hazardous enteric viruses in a short time (10 min) but also unaffected fruit color stability. The simplicity of described CAP design and low-cost inputs (air and electricity) enable the commercial application of inexpensive plasma chambers for continuous surface decontamination of large volumes of raspberries without bringing processing to a standstill.",
publisher = "Elsevier",
journal = "Innovative Food Science and Emerging Technologies",
title = "Efficacy of cold atmospheric plasma for inactivation of viruses on raspberries",
volume = "81",
pages = "103121",
doi = "10.1016/j.ifset.2022.103121"
}

Velebit, B., Milojević, L., Baltić, T., Grković, N., Gummalla, S., Velebit, M., Škoko, I., Mojsova, S.,& Putnik, P.. (2022). Efficacy of cold atmospheric plasma for inactivation of viruses on raspberries. in Innovative Food Science and Emerging Technologies
Elsevier., 81, 103121.
https://doi.org/10.1016/j.ifset.2022.103121

Velebit B, Milojević L, Baltić T, Grković N, Gummalla S, Velebit M, Škoko I, Mojsova S, Putnik P. Efficacy of cold atmospheric plasma for inactivation of viruses on raspberries. in Innovative Food Science and Emerging Technologies. 2022;81:103121.
doi:10.1016/j.ifset.2022.103121 .

Velebit, Branko, Milojević, Lazar, Baltić, Tatjana, Grković, Nevena, Gummalla, Sanjay, Velebit, Marina, Škoko, Ines, Mojsova, Sandra, Putnik, Predrag, "Efficacy of cold atmospheric plasma for inactivation of viruses on raspberries" in Innovative Food Science and Emerging Technologies, 81 (2022):103121,
https://doi.org/10.1016/j.ifset.2022.103121 . .