TY  - JOUR
AU  - Savić Radovanović, Radoslava
AU  - Rajić Savić, Nataša
AU  - Ranin, Lazar
AU  - Smitran, Aleksandra
AU  - Vučković Opavski, Nataša
AU  - Milosavljević Tepavčević, Andreja
AU  - Ranin, Jovana
AU  - Gajić, Ina
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2697
AB  - Due to its ubiquity, ability to form biofilms, and acquire resistance mechanisms, Pseudomonas spp. become one of the major
challenge for healthcare settings and food industry. The aims of this study were to assess the biofilm production of Pseudomonas
spp. recovered from clinical and food specimens and to evaluate their antimicrobial resistance. A total of 108
isolates of Pseudomonas spp. were included in the study, 48 being clinical isolates recovered from patients admitted to four
tertiary care hospitals throughout Serbia and 60 were isolated from the bulk tank milk samples and meat carcasses. Biofilm
production was analyzed by microtiter plate assay. Antimicrobial susceptibility was evaluated by disk diffusion method
according to the CLSI guidelines, while class A and B β-lactamases encoding genes were screened by PCR. A total of 98
(90.7%) strains were biofilm producers (moderate producer: 68, 69.4%; strong producer: 8, 8.2%). Although a slightly higher
percentage of clinical isolates were biofilm producers (91.7%) compared to food isolates (90%), statistical significance was
not observed (P > 0.05). The proportion of carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates was significantly
higher among clinical (42%) isolates compared to food (1.7%) Pseudomonads (P < 0.05). The blaPER and blaNDM genes were
found in ESBL (seven isolates) and MBL (two isolates) production, respectively. In the present study, we confirmed that
biofilm formation was highly present in both clinical and food Pseudomonas spp. irrespective of the prior existence of resistance
genes. Additionally, clinical settings pose a major reservoir of MDR Pseudomonas spp. and especially CRPA isolates.
PB  - Springer
T2  - Current Microbiology
T1  - Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp.
VL  - 77
SP  - 4045
EP  - 4052
DO  - 10.1007/s00284-020-02236-4
ER  - 

@article{
author = "Savić Radovanović, Radoslava and Rajić Savić, Nataša and Ranin, Lazar and Smitran, Aleksandra and Vučković Opavski, Nataša and Milosavljević Tepavčević, Andreja and Ranin, Jovana and Gajić, Ina",
year = "2020",
abstract = "Due to its ubiquity, ability to form biofilms, and acquire resistance mechanisms, Pseudomonas spp. become one of the major
challenge for healthcare settings and food industry. The aims of this study were to assess the biofilm production of Pseudomonas
spp. recovered from clinical and food specimens and to evaluate their antimicrobial resistance. A total of 108
isolates of Pseudomonas spp. were included in the study, 48 being clinical isolates recovered from patients admitted to four
tertiary care hospitals throughout Serbia and 60 were isolated from the bulk tank milk samples and meat carcasses. Biofilm
production was analyzed by microtiter plate assay. Antimicrobial susceptibility was evaluated by disk diffusion method
according to the CLSI guidelines, while class A and B β-lactamases encoding genes were screened by PCR. A total of 98
(90.7%) strains were biofilm producers (moderate producer: 68, 69.4%; strong producer: 8, 8.2%). Although a slightly higher
percentage of clinical isolates were biofilm producers (91.7%) compared to food isolates (90%), statistical significance was
not observed (P > 0.05). The proportion of carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates was significantly
higher among clinical (42%) isolates compared to food (1.7%) Pseudomonads (P < 0.05). The blaPER and blaNDM genes were
found in ESBL (seven isolates) and MBL (two isolates) production, respectively. In the present study, we confirmed that
biofilm formation was highly present in both clinical and food Pseudomonas spp. irrespective of the prior existence of resistance
genes. Additionally, clinical settings pose a major reservoir of MDR Pseudomonas spp. and especially CRPA isolates.",
publisher = "Springer",
journal = "Current Microbiology",
title = "Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp.",
volume = "77",
pages = "4045-4052",
doi = "10.1007/s00284-020-02236-4"
}

Savić Radovanović, R., Rajić Savić, N., Ranin, L., Smitran, A., Vučković Opavski, N., Milosavljević Tepavčević, A., Ranin, J.,& Gajić, I.. (2020). Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp.. in Current Microbiology
Springer., 77, 4045-4052.
https://doi.org/10.1007/s00284-020-02236-4

Savić Radovanović R, Rajić Savić N, Ranin L, Smitran A, Vučković Opavski N, Milosavljević Tepavčević A, Ranin J, Gajić I. Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp.. in Current Microbiology. 2020;77:4045-4052.
doi:10.1007/s00284-020-02236-4 .

Savić Radovanović, Radoslava, Rajić Savić, Nataša, Ranin, Lazar, Smitran, Aleksandra, Vučković Opavski, Nataša, Milosavljević Tepavčević, Andreja, Ranin, Jovana, Gajić, Ina, "Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp." in Current Microbiology, 77 (2020):4045-4052,
https://doi.org/10.1007/s00284-020-02236-4 . .

TY  - JOUR
AU  - Ivanović, Jelena
AU  - Janjić, Jelena
AU  - Mitrović, Radmila
AU  - Krnjaić, Dejan
AU  - Bošković, Marija
AU  - Ranin, Lazar
AU  - Baltić, Milan Ž.
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2128
AB  - Evropska agencija za bezbednost hrane (EFSA - European Food Safety Authority)
objavila je u 2011. godini preporuke za inspekciju trupova svinja. Preporuke se odnose
na najznačajnije biološke opasnosti po zdravlje ljudi koje se mogu naći u mesu.
Pored Salmonella spp., Toxoplasma gondii i Trichinella spp., kao biološka opasnost
navodi se i Yersinia enterocolitica. Yersinia enterocolitica, kao i druge bakterije iz familije
Enterobacteriaceae, ima sposobnost opstanka u spoljašnjoj sredini, u koju dospeva
preko inficiranih životinja. U Evropi, svinje su najčešći asimptomatski nosioci
patogenih sojeva Y. enterocolitica za ljude, posebno soja biotipa 4 (serotipa O:3) i ne
tako učestalog biotipa 2 (serotip O:9 i O:27). Uzročnici su najčešće lokalizovani u
oralnoj duplji, posebno u tonzilama, submaksilarnim limfnim čvorovima, crevima i
fecesu svinja. Pravilan postupak sa trupovima svinja, nakon klanja, može znatno da
smanji nivo kontaminacije mesa svinja. Učestalost Y. enterocolitica kod svinja, kao
rezervoara ovog patogena, veoma varira. Slučajevi jersinioze kod ljudi zabeleženi su,
kako u Evropi, tako i u Japanu, SAD, Nigeriji i Brazilu.
AB  - The European Food Safety Authority (EFSA - European Food Safety Authority)
published in 2011. the recommendations of the inspection of carcasses of pigs. Recommendations
are related to the most significant biological hazards to human
health that can be found in meat. In addition to Salmonella spp., Toxoplasma gondii
and Trichinella spp., as a biological hazard states and Yersinia enterocolitica. Yersinia
enterocolitica, and other bacteria of the Enterobacteriaceae family having ability to
survive in the environment, in which accrues through the infected animals. In Europe,
pigs are the most common asymptomatic carriers of pathogenic strains of Y.
enterocolitica to humans, especially biotype 4 (serotype O: 3) and not so frequent
biotype 2 (serotype O: 9 and O: 27). The causes are usually localized in the oral cavity,
especially in the tonsils, submaxillary lymph nodes, intestines and feces of pigs.
The proper treatment of pig carcasses after slaughter, can significantly reduce the
level of contamination of pig meat. The incidence of Y. enterocolitica in pigs, as reservoirs
of this pathogen varies. Yersiniosis cases in humans have been reported,
both in Europe and in Japan, the United States, Nigeria and Brazil.
PB  - Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“
T2  - Veterinarski žurnal Republike Srpske
T1  - Yersinia enterocolitica u mesu svinja - rizik za bezbednost hrane
T1  - Yersinia enterocolitica in the pig meat: A risk for food safety
VL  - 16
IS  - 1
SP  - 104
EP  - 120
DO  - 10.7251/VETJ1601105I
ER  - 

@article{
author = "Ivanović, Jelena and Janjić, Jelena and Mitrović, Radmila and Krnjaić, Dejan and Bošković, Marija and Ranin, Lazar and Baltić, Milan Ž.",
year = "2016",
abstract = "Evropska agencija za bezbednost hrane (EFSA - European Food Safety Authority)
objavila je u 2011. godini preporuke za inspekciju trupova svinja. Preporuke se odnose
na najznačajnije biološke opasnosti po zdravlje ljudi koje se mogu naći u mesu.
Pored Salmonella spp., Toxoplasma gondii i Trichinella spp., kao biološka opasnost
navodi se i Yersinia enterocolitica. Yersinia enterocolitica, kao i druge bakterije iz familije
Enterobacteriaceae, ima sposobnost opstanka u spoljašnjoj sredini, u koju dospeva
preko inficiranih životinja. U Evropi, svinje su najčešći asimptomatski nosioci
patogenih sojeva Y. enterocolitica za ljude, posebno soja biotipa 4 (serotipa O:3) i ne
tako učestalog biotipa 2 (serotip O:9 i O:27). Uzročnici su najčešće lokalizovani u
oralnoj duplji, posebno u tonzilama, submaksilarnim limfnim čvorovima, crevima i
fecesu svinja. Pravilan postupak sa trupovima svinja, nakon klanja, može znatno da
smanji nivo kontaminacije mesa svinja. Učestalost Y. enterocolitica kod svinja, kao
rezervoara ovog patogena, veoma varira. Slučajevi jersinioze kod ljudi zabeleženi su,
kako u Evropi, tako i u Japanu, SAD, Nigeriji i Brazilu., The European Food Safety Authority (EFSA - European Food Safety Authority)
published in 2011. the recommendations of the inspection of carcasses of pigs. Recommendations
are related to the most significant biological hazards to human
health that can be found in meat. In addition to Salmonella spp., Toxoplasma gondii
and Trichinella spp., as a biological hazard states and Yersinia enterocolitica. Yersinia
enterocolitica, and other bacteria of the Enterobacteriaceae family having ability to
survive in the environment, in which accrues through the infected animals. In Europe,
pigs are the most common asymptomatic carriers of pathogenic strains of Y.
enterocolitica to humans, especially biotype 4 (serotype O: 3) and not so frequent
biotype 2 (serotype O: 9 and O: 27). The causes are usually localized in the oral cavity,
especially in the tonsils, submaxillary lymph nodes, intestines and feces of pigs.
The proper treatment of pig carcasses after slaughter, can significantly reduce the
level of contamination of pig meat. The incidence of Y. enterocolitica in pigs, as reservoirs
of this pathogen varies. Yersiniosis cases in humans have been reported,
both in Europe and in Japan, the United States, Nigeria and Brazil.",
publisher = "Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“",
journal = "Veterinarski žurnal Republike Srpske",
title = "Yersinia enterocolitica u mesu svinja - rizik za bezbednost hrane, Yersinia enterocolitica in the pig meat: A risk for food safety",
volume = "16",
number = "1",
pages = "104-120",
doi = "10.7251/VETJ1601105I"
}

Ivanović, J., Janjić, J., Mitrović, R., Krnjaić, D., Bošković, M., Ranin, L.,& Baltić, M. Ž.. (2016). Yersinia enterocolitica u mesu svinja - rizik za bezbednost hrane. in Veterinarski žurnal Republike Srpske
Banja Luka : PI Veterinary Institute Republic of Srpska „Dr. Vaso Butozan“., 16(1), 104-120.
https://doi.org/10.7251/VETJ1601105I

Ivanović J, Janjić J, Mitrović R, Krnjaić D, Bošković M, Ranin L, Baltić MŽ. Yersinia enterocolitica u mesu svinja - rizik za bezbednost hrane. in Veterinarski žurnal Republike Srpske. 2016;16(1):104-120.
doi:10.7251/VETJ1601105I .

Ivanović, Jelena, Janjić, Jelena, Mitrović, Radmila, Krnjaić, Dejan, Bošković, Marija, Ranin, Lazar, Baltić, Milan Ž., "Yersinia enterocolitica u mesu svinja - rizik za bezbednost hrane" in Veterinarski žurnal Republike Srpske, 16, no. 1 (2016):104-120,
https://doi.org/10.7251/VETJ1601105I . .