TY  - JOUR
AU  - Vasić, Ana
AU  - Zdravković, Nemanja
AU  - Anita, Dragos
AU  - Bojkovski, Jovan
AU  - Marinov, Mihai
AU  - Mathis, Alexander
AU  - Niculaua, Marius
AU  - Oslobanu, Elena Luanda
AU  - Pavlović, Ivan
AU  - Petrić, Dusan
AU  - Pflueger, Valentin
AU  - Pudar, Dubravka
AU  - Savuta, Gheorghe
AU  - Simeunović, Predrag
AU  - Veronesi, Eva
AU  - Silaghi, Cornelia
AU  - Anita, Adriana
AU  - Anton, Ioana Alexandra
AU  - Cimpan, Andrei
AU  - Ciuca, Lavinia
AU  - Crivei, Luciana
AU  - Cojkić, Aleksandar
AU  - Davitkov, Darko
AU  - Drašković, Vladimir
AU  - Gajić, Bojan
AU  - Glavinić, Uroš
AU  - Ivanescu, Maria-Larisa
AU  - Kavran, Mihaela
AU  - Lupu, Andrei-Cristian
AU  - Mindru, Raluca
AU  - Porea, Daniela
AU  - Prodanović, Radiša
AU  - Radanović, Oliver
AU  - Raileanu, Cristian
AU  - Raileanu, Stefan
AU  - Ristanić, Marko
AU  - Roman, Constantin
AU  - Stanišić, Ljubodrag
AU  - Vaselek, Slavica
AU  - Đurić, Miloje
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1740
AB  - BackgroundCulicoides (Diptera: Ceratopogonidae) is a genus of small biting midges (also known as no-see ums) that currently includes 1368 described species. They are proven or suspected vectors for important pathogens affecting animals such as bluetongue virus (BTV) and Schmallenberg virus (SBV). Currently little information is available on the species of Culicoides present in Serbia. Thus, the aim of this study was to examine species diversity, host preference and the presence of BTV and SBV RNA in Culicoides from the Stara Planina Nature Park in south-eastern Serbia.ResultsIn total 19,887 individual Culicoides were collected during three nights of trapping at two farm sites and pooled into six groups (Obsoletus group, Pulicaris group, Others group and further each group according to the blood-feeding status to freshly engorged and non-engorged). Species identification was done on subsamples of 592 individual Culicoides specimens by morphological and molecular methods (MALDI-TOF mass spectrometry and PCR/sequencing). At least 22 Culicoides species were detected. Four animal species (cow, sheep, goat and common blackbird) as well as humans were identified as hosts of Culicoides biting midges. The screening of 8291 Culicoides specimens in 99 pools for the presence of BTV and SBV RNA by reverse-transcription quantitative PCR were negative.ConclusionsThe biodiversity of Culicoides species in the natural reserve Stara Planina was high with at least 22 species present. The presence of C. imicola Kieffer was not recorded in this area. Culicoides showed opportunistic feeding behaviour as determined by host preference. The absence of SBV and BTV viral RNA correlates with the absence of clinical disease in the field during the time of sampling. These data are the direct outcome of a training programme within the Institutional Partnership Project AMSAR: Arbovirus monitoring, research and surveillance-capacity building on mosquitoes and biting midges funded by the programme SCOPES of the Swiss National Science Foundation.
PB  - BMC, London
T2  - Parasites & Vectors
T1  - Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia
VL  - 12
SP  - 61
DO  - 10.1186/s13071-019-3292-3
ER  - 

@article{
author = "Vasić, Ana and Zdravković, Nemanja and Anita, Dragos and Bojkovski, Jovan and Marinov, Mihai and Mathis, Alexander and Niculaua, Marius and Oslobanu, Elena Luanda and Pavlović, Ivan and Petrić, Dusan and Pflueger, Valentin and Pudar, Dubravka and Savuta, Gheorghe and Simeunović, Predrag and Veronesi, Eva and Silaghi, Cornelia and Anita, Adriana and Anton, Ioana Alexandra and Cimpan, Andrei and Ciuca, Lavinia and Crivei, Luciana and Cojkić, Aleksandar and Davitkov, Darko and Drašković, Vladimir and Gajić, Bojan and Glavinić, Uroš and Ivanescu, Maria-Larisa and Kavran, Mihaela and Lupu, Andrei-Cristian and Mindru, Raluca and Porea, Daniela and Prodanović, Radiša and Radanović, Oliver and Raileanu, Cristian and Raileanu, Stefan and Ristanić, Marko and Roman, Constantin and Stanišić, Ljubodrag and Vaselek, Slavica and Đurić, Miloje",
year = "2019",
abstract = "BackgroundCulicoides (Diptera: Ceratopogonidae) is a genus of small biting midges (also known as no-see ums) that currently includes 1368 described species. They are proven or suspected vectors for important pathogens affecting animals such as bluetongue virus (BTV) and Schmallenberg virus (SBV). Currently little information is available on the species of Culicoides present in Serbia. Thus, the aim of this study was to examine species diversity, host preference and the presence of BTV and SBV RNA in Culicoides from the Stara Planina Nature Park in south-eastern Serbia.ResultsIn total 19,887 individual Culicoides were collected during three nights of trapping at two farm sites and pooled into six groups (Obsoletus group, Pulicaris group, Others group and further each group according to the blood-feeding status to freshly engorged and non-engorged). Species identification was done on subsamples of 592 individual Culicoides specimens by morphological and molecular methods (MALDI-TOF mass spectrometry and PCR/sequencing). At least 22 Culicoides species were detected. Four animal species (cow, sheep, goat and common blackbird) as well as humans were identified as hosts of Culicoides biting midges. The screening of 8291 Culicoides specimens in 99 pools for the presence of BTV and SBV RNA by reverse-transcription quantitative PCR were negative.ConclusionsThe biodiversity of Culicoides species in the natural reserve Stara Planina was high with at least 22 species present. The presence of C. imicola Kieffer was not recorded in this area. Culicoides showed opportunistic feeding behaviour as determined by host preference. The absence of SBV and BTV viral RNA correlates with the absence of clinical disease in the field during the time of sampling. These data are the direct outcome of a training programme within the Institutional Partnership Project AMSAR: Arbovirus monitoring, research and surveillance-capacity building on mosquitoes and biting midges funded by the programme SCOPES of the Swiss National Science Foundation.",
publisher = "BMC, London",
journal = "Parasites & Vectors",
title = "Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia",
volume = "12",
pages = "61",
doi = "10.1186/s13071-019-3292-3"
}

Vasić, A., Zdravković, N., Anita, D., Bojkovski, J., Marinov, M., Mathis, A., Niculaua, M., Oslobanu, E. L., Pavlović, I., Petrić, D., Pflueger, V., Pudar, D., Savuta, G., Simeunović, P., Veronesi, E., Silaghi, C., Anita, A., Anton, I. A., Cimpan, A., Ciuca, L., Crivei, L., Cojkić, A., Davitkov, D., Drašković, V., Gajić, B., Glavinić, U., Ivanescu, M., Kavran, M., Lupu, A., Mindru, R., Porea, D., Prodanović, R., Radanović, O., Raileanu, C., Raileanu, S., Ristanić, M., Roman, C., Stanišić, L., Vaselek, S.,& Đurić, M.. (2019). Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia. in Parasites & Vectors
BMC, London., 12, 61.
https://doi.org/10.1186/s13071-019-3292-3

Vasić A, Zdravković N, Anita D, Bojkovski J, Marinov M, Mathis A, Niculaua M, Oslobanu EL, Pavlović I, Petrić D, Pflueger V, Pudar D, Savuta G, Simeunović P, Veronesi E, Silaghi C, Anita A, Anton IA, Cimpan A, Ciuca L, Crivei L, Cojkić A, Davitkov D, Drašković V, Gajić B, Glavinić U, Ivanescu M, Kavran M, Lupu A, Mindru R, Porea D, Prodanović R, Radanović O, Raileanu C, Raileanu S, Ristanić M, Roman C, Stanišić L, Vaselek S, Đurić M. Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia. in Parasites & Vectors. 2019;12:61.
doi:10.1186/s13071-019-3292-3 .

Vasić, Ana, Zdravković, Nemanja, Anita, Dragos, Bojkovski, Jovan, Marinov, Mihai, Mathis, Alexander, Niculaua, Marius, Oslobanu, Elena Luanda, Pavlović, Ivan, Petrić, Dusan, Pflueger, Valentin, Pudar, Dubravka, Savuta, Gheorghe, Simeunović, Predrag, Veronesi, Eva, Silaghi, Cornelia, Anita, Adriana, Anton, Ioana Alexandra, Cimpan, Andrei, Ciuca, Lavinia, Crivei, Luciana, Cojkić, Aleksandar, Davitkov, Darko, Drašković, Vladimir, Gajić, Bojan, Glavinić, Uroš, Ivanescu, Maria-Larisa, Kavran, Mihaela, Lupu, Andrei-Cristian, Mindru, Raluca, Porea, Daniela, Prodanović, Radiša, Radanović, Oliver, Raileanu, Cristian, Raileanu, Stefan, Ristanić, Marko, Roman, Constantin, Stanišić, Ljubodrag, Vaselek, Slavica, Đurić, Miloje, "Species diversity, host preference and arbovirus detection of Culicoides (Diptera: Ceratopogonidae) in south-eastern Serbia" in Parasites & Vectors, 12 (2019):61,
https://doi.org/10.1186/s13071-019-3292-3 . .

TY  - JOUR
AU  - Petrović, Tamaš
AU  - Šekler, Milanko
AU  - Petrić, Dusan
AU  - Lazić, Sava
AU  - Debeljak, Zoran
AU  - Vidanović, Dejan
AU  - Ignjatović-Čupina, Aleksandra
AU  - Lazić, Gospava
AU  - Lupulović, Diana
AU  - Kolarević, Migo
AU  - Plavsić, Budimir
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1632
AB  - Studies conducted during the past few years have confirmed active West Nile virus (WNV) circulation in Serbia. Based on these studies and the epidemiological situation, the Veterinary Directorate of the Ministry of Agriculture and Environmental Protection launched national WNV surveillance programmes in 2014 and 2015. The programmes encompassed the territory of Serbia and were conducted by the veterinary service in collaboration with entomologists and ornithologists. The objective of the programmes was early detection of WNV and timely reporting to the public health service and local authorities to increase both clinical and mosquito control preparedness. The WNV surveillance programmes were based on direct and indirect surveillance of the presence of WNV by the serological testing of initially seronegative sentinel horses and chickens as well as through viral detection in pooled mosquito and wild bird samples. The most intense WNV circulation was observed in all seven districts of Vojvodina Province (northern Serbia) and Belgrade City, where most of the positive samples were detected among sentinel animals, mosquitoes and wild birds. The West Nile virus surveillance programmes in 2014 and 2015 showed satisfactory results in their capacity to indicate the spatial distribution of the risk for humans and their sensitivity to early detect viral circulation at the enzootic level. Most of the human cases were preceded by the detection of WNV circulation as part of the surveillance programmes. According to the existing data, it can be reasonably assumed that WNV infection, now an endemic infection in Serbia, will continue to present a significant problem for the veterinary service and public health.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Methodology and results of integrated WNV surveillance programmes in Serbia
VL  - 13
IS  - 4
SP  - e0195439
DO  - 10.1371/journal.pone.0195439
ER  - 

@article{
author = "Petrović, Tamaš and Šekler, Milanko and Petrić, Dusan and Lazić, Sava and Debeljak, Zoran and Vidanović, Dejan and Ignjatović-Čupina, Aleksandra and Lazić, Gospava and Lupulović, Diana and Kolarević, Migo and Plavsić, Budimir",
year = "2018",
abstract = "Studies conducted during the past few years have confirmed active West Nile virus (WNV) circulation in Serbia. Based on these studies and the epidemiological situation, the Veterinary Directorate of the Ministry of Agriculture and Environmental Protection launched national WNV surveillance programmes in 2014 and 2015. The programmes encompassed the territory of Serbia and were conducted by the veterinary service in collaboration with entomologists and ornithologists. The objective of the programmes was early detection of WNV and timely reporting to the public health service and local authorities to increase both clinical and mosquito control preparedness. The WNV surveillance programmes were based on direct and indirect surveillance of the presence of WNV by the serological testing of initially seronegative sentinel horses and chickens as well as through viral detection in pooled mosquito and wild bird samples. The most intense WNV circulation was observed in all seven districts of Vojvodina Province (northern Serbia) and Belgrade City, where most of the positive samples were detected among sentinel animals, mosquitoes and wild birds. The West Nile virus surveillance programmes in 2014 and 2015 showed satisfactory results in their capacity to indicate the spatial distribution of the risk for humans and their sensitivity to early detect viral circulation at the enzootic level. Most of the human cases were preceded by the detection of WNV circulation as part of the surveillance programmes. According to the existing data, it can be reasonably assumed that WNV infection, now an endemic infection in Serbia, will continue to present a significant problem for the veterinary service and public health.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Methodology and results of integrated WNV surveillance programmes in Serbia",
volume = "13",
number = "4",
pages = "e0195439",
doi = "10.1371/journal.pone.0195439"
}

Petrović, T., Šekler, M., Petrić, D., Lazić, S., Debeljak, Z., Vidanović, D., Ignjatović-Čupina, A., Lazić, G., Lupulović, D., Kolarević, M.,& Plavsić, B.. (2018). Methodology and results of integrated WNV surveillance programmes in Serbia. in PLoS One
Public Library Science, San Francisco., 13(4), e0195439.
https://doi.org/10.1371/journal.pone.0195439

Petrović T, Šekler M, Petrić D, Lazić S, Debeljak Z, Vidanović D, Ignjatović-Čupina A, Lazić G, Lupulović D, Kolarević M, Plavsić B. Methodology and results of integrated WNV surveillance programmes in Serbia. in PLoS One. 2018;13(4):e0195439.
doi:10.1371/journal.pone.0195439 .

Petrović, Tamaš, Šekler, Milanko, Petrić, Dusan, Lazić, Sava, Debeljak, Zoran, Vidanović, Dejan, Ignjatović-Čupina, Aleksandra, Lazić, Gospava, Lupulović, Diana, Kolarević, Migo, Plavsić, Budimir, "Methodology and results of integrated WNV surveillance programmes in Serbia" in PLoS One, 13, no. 4 (2018):e0195439,
https://doi.org/10.1371/journal.pone.0195439 . .