TY  - CONF
AU  - Vasilev, Saša
AU  - Majstorović, Ivana
AU  - Vučević, Dragana
AU  - Gašić, Sonja
AU  - Vasilijić, S.
AU  - Bufan, Biljana
AU  - Ćupić, Vitomir
AU  - Colić, M.
PY  - 2005
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/333
PB  - Elsevier Ireland Ltd, Clare
C3  - Toxicology Letters
T1  - The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro
VL  - 158
SP  - S229
EP  - S229
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_333
ER  - 

@conference{
author = "Vasilev, Saša and Majstorović, Ivana and Vučević, Dragana and Gašić, Sonja and Vasilijić, S. and Bufan, Biljana and Ćupić, Vitomir and Colić, M.",
year = "2005",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Toxicology Letters",
title = "The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro",
volume = "158",
pages = "S229-S229",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_333"
}

Vasilev, S., Majstorović, I., Vučević, D., Gašić, S., Vasilijić, S., Bufan, B., Ćupić, V.,& Colić, M.. (2005). The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro. in Toxicology Letters
Elsevier Ireland Ltd, Clare., 158, S229-S229.
https://hdl.handle.net/21.15107/rcub_veterinar_333

Vasilev S, Majstorović I, Vučević D, Gašić S, Vasilijić S, Bufan B, Ćupić V, Colić M. The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro. in Toxicology Letters. 2005;158:S229-S229.
https://hdl.handle.net/21.15107/rcub_veterinar_333 .

Vasilev, Saša, Majstorović, Ivana, Vučević, Dragana, Gašić, Sonja, Vasilijić, S., Bufan, Biljana, Ćupić, Vitomir, Colić, M., "The effect of NCX 4016 and NCX 4040, two nitric oxide-donating aspirin derivatives, on apoptosis of neutrophil granulocytes in vitro" in Toxicology Letters, 158 (2005):S229-S229,
https://hdl.handle.net/21.15107/rcub_veterinar_333 .

TY  - JOUR
AU  - Ćupić, Vitomir
AU  - Colić, M
AU  - Jandrić, D.
AU  - Milojković, B
AU  - Varagić, Vladislav M.
PY  - 2003
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/224
AB  - In our previous experiments, we demonstrated that xylazine, an alpha(2)-adrenergic agonist, stimulated proliferation of thymocytes triggered by concanavalin A. In contrast, higher concentrations of xylazine were inhibitory. In this work, we studied the mechanisms involved in immunosuppression of xylazine and found that the compound at concentrations between 100 muM and 500 muM induced apoptosis of rat thymocytes in vitro. In addition, xylazine at concentrations higher than 50 muM also induced apoptosis of a thymocyte hybridoma (BWRT8) and increased apoptosis of the line triggered by T cell receptor (TCR) cross-linking. Apoptosis was confirmed by morphological analysis staining with merocyanine 540 and propidium iodide and in cases of BWRT8 by fragmentation of DNA. The mechanisms of xylazine-induced apoptosis of the BWRT8 hybridoma were further examined. We demonstrated that the process in both nonactivated and activated (TCR cross-linking) BWRT8 cells was not prevented by yohimbine (a selective alpha-adrenergic antagonist) and by antibodies to Fas and Fas-L. In contrast, cell death was completely blocked by a caspase inhibitor Z-Val-Ala-Asp (OMe)-CH2F. Cyclosporine, a calcineurin blocker partly inhibited the xylazine-induced apoptosis of activated BWRT8 cells.
PB  - Prous Science, Sau-Thomson Reuters, Barcelona
T2  - Methods and Findings in Experimental and Clinical Pharmacology
T1  - Xylazine, an alpha(2)-adrenergic agonist, induces apoptosis of rat thymocytes and a thymocyte hybridoma line in vitro
VL  - 25
IS  - 1
SP  - 5
EP  - 10
DO  - 10.1358/mf.2003.25.1.772541
ER  - 

@article{
author = "Ćupić, Vitomir and Colić, M and Jandrić, D. and Milojković, B and Varagić, Vladislav M.",
year = "2003",
abstract = "In our previous experiments, we demonstrated that xylazine, an alpha(2)-adrenergic agonist, stimulated proliferation of thymocytes triggered by concanavalin A. In contrast, higher concentrations of xylazine were inhibitory. In this work, we studied the mechanisms involved in immunosuppression of xylazine and found that the compound at concentrations between 100 muM and 500 muM induced apoptosis of rat thymocytes in vitro. In addition, xylazine at concentrations higher than 50 muM also induced apoptosis of a thymocyte hybridoma (BWRT8) and increased apoptosis of the line triggered by T cell receptor (TCR) cross-linking. Apoptosis was confirmed by morphological analysis staining with merocyanine 540 and propidium iodide and in cases of BWRT8 by fragmentation of DNA. The mechanisms of xylazine-induced apoptosis of the BWRT8 hybridoma were further examined. We demonstrated that the process in both nonactivated and activated (TCR cross-linking) BWRT8 cells was not prevented by yohimbine (a selective alpha-adrenergic antagonist) and by antibodies to Fas and Fas-L. In contrast, cell death was completely blocked by a caspase inhibitor Z-Val-Ala-Asp (OMe)-CH2F. Cyclosporine, a calcineurin blocker partly inhibited the xylazine-induced apoptosis of activated BWRT8 cells.",
publisher = "Prous Science, Sau-Thomson Reuters, Barcelona",
journal = "Methods and Findings in Experimental and Clinical Pharmacology",
title = "Xylazine, an alpha(2)-adrenergic agonist, induces apoptosis of rat thymocytes and a thymocyte hybridoma line in vitro",
volume = "25",
number = "1",
pages = "5-10",
doi = "10.1358/mf.2003.25.1.772541"
}

Ćupić, V., Colić, M., Jandrić, D., Milojković, B.,& Varagić, V. M.. (2003). Xylazine, an alpha(2)-adrenergic agonist, induces apoptosis of rat thymocytes and a thymocyte hybridoma line in vitro. in Methods and Findings in Experimental and Clinical Pharmacology
Prous Science, Sau-Thomson Reuters, Barcelona., 25(1), 5-10.
https://doi.org/10.1358/mf.2003.25.1.772541

Ćupić V, Colić M, Jandrić D, Milojković B, Varagić VM. Xylazine, an alpha(2)-adrenergic agonist, induces apoptosis of rat thymocytes and a thymocyte hybridoma line in vitro. in Methods and Findings in Experimental and Clinical Pharmacology. 2003;25(1):5-10.
doi:10.1358/mf.2003.25.1.772541 .

Ćupić, Vitomir, Colić, M, Jandrić, D., Milojković, B, Varagić, Vladislav M., "Xylazine, an alpha(2)-adrenergic agonist, induces apoptosis of rat thymocytes and a thymocyte hybridoma line in vitro" in Methods and Findings in Experimental and Clinical Pharmacology, 25, no. 1 (2003):5-10,
https://doi.org/10.1358/mf.2003.25.1.772541 . .

TY  - JOUR
AU  - Ćupić, Vitomir
AU  - Colić, M
AU  - Pavicić, L
AU  - Vučević, Dragana
AU  - Varagić, Vladislav M.
PY  - 2001
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/179
AB  - Xylazine is an adrenergic alpha (2) agonist, which is used in veterinary medicine as a sedative and anesthetic agent. In this work we found that xylazine administered in vivo at a dose of 2.5 mg/kg enhanced spleen cell proliferation and interleukin 2 (IL-2) production in cultures stimulated with concanavalin A (Con A), whereas doses of 10 and 25 mg/kg were inhibitory. A similar stimulatory (10 muM) and inhibitory (50-500 muM) effect on splenocyte proliferation and IL-2 production was observed in vitro. Clonidine. another alpha (2) adrenergic agonist, only had a stimulatory proliferative effect on splenocytes. Yohimbine, an alpha (2) adrenergic antagonist, abrogated the stimulatory action of both clonidine and xylazine, but not the suppressive proliferative activity of xylazine in vitro. The inhibited proliferation of splenocytes to Con A correlated with increased apoptosis of T cells. The apoptosis was not blocked by yohimbine or antibodies to Fas and Fas-L. N-Nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) synthase. enhanced proliferation of splenocytes to Con Al partly abrogated the inhibitory effect of xylazine in the proliferation assay. and, only at high concentration (1000 muM), partly suppressed apoptosis of lymphocytes. The enhancing effect of L-NAME on the Con A-induced proliferation of splenocytes correlated with decreased NO production. However, decreased NO production observed in cultures with xylazine was followed by both decreased lymphocyte proliferation and apoptosis. Cumulatively, these results suggest that the immunosuppressive properties of xylazine on splenocytes in vitro are due to increased apoptosis of lymphocytes, predominantly involve NO-independent pathways, and are probably independent of its action through alpha (2) adrenoreceptors.
PB  - Elsevier, Amsterdam
T2  - Journal of Neuroimmunology
T1  - Immunomodulatory effect of xylazine, an alpha(2) adrenergic agonist, on rat spleen cells in culture
VL  - 113
IS  - 1
SP  - 19
EP  - 29
DO  - 10.1016/S0165-5728(00)00370-2
ER  - 

@article{
author = "Ćupić, Vitomir and Colić, M and Pavicić, L and Vučević, Dragana and Varagić, Vladislav M.",
year = "2001",
abstract = "Xylazine is an adrenergic alpha (2) agonist, which is used in veterinary medicine as a sedative and anesthetic agent. In this work we found that xylazine administered in vivo at a dose of 2.5 mg/kg enhanced spleen cell proliferation and interleukin 2 (IL-2) production in cultures stimulated with concanavalin A (Con A), whereas doses of 10 and 25 mg/kg were inhibitory. A similar stimulatory (10 muM) and inhibitory (50-500 muM) effect on splenocyte proliferation and IL-2 production was observed in vitro. Clonidine. another alpha (2) adrenergic agonist, only had a stimulatory proliferative effect on splenocytes. Yohimbine, an alpha (2) adrenergic antagonist, abrogated the stimulatory action of both clonidine and xylazine, but not the suppressive proliferative activity of xylazine in vitro. The inhibited proliferation of splenocytes to Con A correlated with increased apoptosis of T cells. The apoptosis was not blocked by yohimbine or antibodies to Fas and Fas-L. N-Nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) synthase. enhanced proliferation of splenocytes to Con Al partly abrogated the inhibitory effect of xylazine in the proliferation assay. and, only at high concentration (1000 muM), partly suppressed apoptosis of lymphocytes. The enhancing effect of L-NAME on the Con A-induced proliferation of splenocytes correlated with decreased NO production. However, decreased NO production observed in cultures with xylazine was followed by both decreased lymphocyte proliferation and apoptosis. Cumulatively, these results suggest that the immunosuppressive properties of xylazine on splenocytes in vitro are due to increased apoptosis of lymphocytes, predominantly involve NO-independent pathways, and are probably independent of its action through alpha (2) adrenoreceptors.",
publisher = "Elsevier, Amsterdam",
journal = "Journal of Neuroimmunology",
title = "Immunomodulatory effect of xylazine, an alpha(2) adrenergic agonist, on rat spleen cells in culture",
volume = "113",
number = "1",
pages = "19-29",
doi = "10.1016/S0165-5728(00)00370-2"
}

Ćupić, V., Colić, M., Pavicić, L., Vučević, D.,& Varagić, V. M.. (2001). Immunomodulatory effect of xylazine, an alpha(2) adrenergic agonist, on rat spleen cells in culture. in Journal of Neuroimmunology
Elsevier, Amsterdam., 113(1), 19-29.
https://doi.org/10.1016/S0165-5728(00)00370-2

Ćupić V, Colić M, Pavicić L, Vučević D, Varagić VM. Immunomodulatory effect of xylazine, an alpha(2) adrenergic agonist, on rat spleen cells in culture. in Journal of Neuroimmunology. 2001;113(1):19-29.
doi:10.1016/S0165-5728(00)00370-2 .

Ćupić, Vitomir, Colić, M, Pavicić, L, Vučević, Dragana, Varagić, Vladislav M., "Immunomodulatory effect of xylazine, an alpha(2) adrenergic agonist, on rat spleen cells in culture" in Journal of Neuroimmunology, 113, no. 1 (2001):19-29,
https://doi.org/10.1016/S0165-5728(00)00370-2 . .

TY  - JOUR
AU  - Colić, M
AU  - Ćupić, Vitomir
AU  - Pavicić, L
AU  - Vučević, Dragana
AU  - Varagić, Vladislav M.
PY  - 2000
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/137
AB  - The effect of xylazine, an alpha (2)-adrenergic agonist, on proliferation of rat thymocytes in vivo and in vitro was examined. It was found that the agonist administered to rats in vivo at doses of 2.5 mg/kg and 5 mg/kg stimulated thymocyte proliferation to suboptimal (0.625 mug/ml) concentrations of concanavalin A (Con A). A similar effect was confirmed in vitro when lower concentrations of xylazine (5 muM) were added to cultures of thymic cells from intact animals in the presence of both suboptimal and optimal (2.5 mug/ml) Con A concentrations. Higher doses in vivo (25 mg/kg) and in vitro (50 muM, 100 muM and 250 muM) significantly inhibited proliferation of thymocytes to Con A. The phenomenon was followed by a decrease in interleukin-2 (IL-2) production (in vivo and in vitro) and down-regulation of IL-2 receptor alpha (IL-2R alpha) expression (in vitro). The exogenous IL-2 completely restored the inhibitory effect of xylazine in vivo was observed. Stimulatory effect of xylazine on proliferation of thymocytes was probably mediated through alpha (2)-adrenoreceptors since it was blocked by yohimbine, an alpha (2)-adrenoreceptor antagonist. It seems that the pathways involved in inhibition of thymocyte proliferation by xylazine are more complex because the xylazine-suppressed thymocyte proliferation was potentiated by yohimine.
PB  - Prous Science, Sau-Thomson Reuters, Barcelona
T2  - Methods and Findings in Experimental and Clinical Pharmacology
T1  - Xylazine, an alpha(2)-adrenergic agonist, modulates proliferation of rat thymocytes in vivo and in vitro
VL  - 22
IS  - 7
SP  - 557
EP  - 562
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_137
ER  - 

@article{
author = "Colić, M and Ćupić, Vitomir and Pavicić, L and Vučević, Dragana and Varagić, Vladislav M.",
year = "2000",
abstract = "The effect of xylazine, an alpha (2)-adrenergic agonist, on proliferation of rat thymocytes in vivo and in vitro was examined. It was found that the agonist administered to rats in vivo at doses of 2.5 mg/kg and 5 mg/kg stimulated thymocyte proliferation to suboptimal (0.625 mug/ml) concentrations of concanavalin A (Con A). A similar effect was confirmed in vitro when lower concentrations of xylazine (5 muM) were added to cultures of thymic cells from intact animals in the presence of both suboptimal and optimal (2.5 mug/ml) Con A concentrations. Higher doses in vivo (25 mg/kg) and in vitro (50 muM, 100 muM and 250 muM) significantly inhibited proliferation of thymocytes to Con A. The phenomenon was followed by a decrease in interleukin-2 (IL-2) production (in vivo and in vitro) and down-regulation of IL-2 receptor alpha (IL-2R alpha) expression (in vitro). The exogenous IL-2 completely restored the inhibitory effect of xylazine in vivo was observed. Stimulatory effect of xylazine on proliferation of thymocytes was probably mediated through alpha (2)-adrenoreceptors since it was blocked by yohimbine, an alpha (2)-adrenoreceptor antagonist. It seems that the pathways involved in inhibition of thymocyte proliferation by xylazine are more complex because the xylazine-suppressed thymocyte proliferation was potentiated by yohimine.",
publisher = "Prous Science, Sau-Thomson Reuters, Barcelona",
journal = "Methods and Findings in Experimental and Clinical Pharmacology",
title = "Xylazine, an alpha(2)-adrenergic agonist, modulates proliferation of rat thymocytes in vivo and in vitro",
volume = "22",
number = "7",
pages = "557-562",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_137"
}

Colić, M., Ćupić, V., Pavicić, L., Vučević, D.,& Varagić, V. M.. (2000). Xylazine, an alpha(2)-adrenergic agonist, modulates proliferation of rat thymocytes in vivo and in vitro. in Methods and Findings in Experimental and Clinical Pharmacology
Prous Science, Sau-Thomson Reuters, Barcelona., 22(7), 557-562.
https://hdl.handle.net/21.15107/rcub_veterinar_137

Colić M, Ćupić V, Pavicić L, Vučević D, Varagić VM. Xylazine, an alpha(2)-adrenergic agonist, modulates proliferation of rat thymocytes in vivo and in vitro. in Methods and Findings in Experimental and Clinical Pharmacology. 2000;22(7):557-562.
https://hdl.handle.net/21.15107/rcub_veterinar_137 .

Colić, M, Ćupić, Vitomir, Pavicić, L, Vučević, Dragana, Varagić, Vladislav M., "Xylazine, an alpha(2)-adrenergic agonist, modulates proliferation of rat thymocytes in vivo and in vitro" in Methods and Findings in Experimental and Clinical Pharmacology, 22, no. 7 (2000):557-562,
https://hdl.handle.net/21.15107/rcub_veterinar_137 .