TY  - JOUR
AU  - Pérez-Ramírez, Elisa
AU  - Cano-Gómez, Cristina
AU  - Llorente, Francisco
AU  - Vodica, Ani
AU  - Veljović, Ljubiša
AU  - Toklikishvilli, Natela
AU  - Sherifi, Kurtesh
AU  - Sghaier, Soufien
AU  - Omani, Amel
AU  - Kustura, Aida
AU  - Krstevski, Kiril
AU  - Karayel-Hacioglu, Ilke
AU  - Hagag, Naglaa Mohamed
AU  - Hage, Jeanne El
AU  - Davdyan, Hasmik
AU  - Bintarif, Mohd Saddam
AU  - Adžić, Bojan
AU  - Abouchoaib, Nabil
AU  - Jiménez-Clavero, Miguel Ángel
AU  - Fernández-Pinero, Jovita
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2019
AB  - The increasing incidence of West Nile virus (WNV) in the Euro-Mediterranean area warrants the implementation of effective surveillance programs in animals. A crucial step in the fight against the disease is the evaluation of the capacity of the veterinary labs to accurately detect the infection in animal populations. In this context, the animal virology network of the MediLabSecure project organized an external quality assessment (EQA) to evaluate the WNV molecular and serological diagnostic capacities of beneficiary veterinary labs. Laboratories from 17 Mediterranean and Black Sea countries participated. The results of the triplex real time RT-PCR for simultaneous detection and differentiation of WNV lineage 1 (L1), lineage 2 (L2) and Usutu virus (USUV) were highly satisfactory, especially for L1 and L2, with detection rates of 97.9% and 100%, respectively. For USUV, 75% of the labs reported correct results. More limitations were observed for the generic detection of flaviviruses using conventional reverse-transcription polymerase chain reaction (RT-PCR), since only 46.1% reported correct results in the whole panel. As regards the serological panel, the results were excellent for the generic detection of WNV antibodies. More variability was observed for the specific detection of IgM antibodies with a higher percentage of incorrect results mainly in samples with low titers. This EQA provides a good overview of the WNV (and USUV) diagnostic performance of the involved veterinary labs and demonstrates that the implemented training program was successful in upgrading their diagnostic capacities.
PB  - MDPI AG
T2  - Pathogens
T1  - Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions
VL  - 9
IS  - 12
SP  - 1
EP  - 20
DO  - 10.3390/pathogens9121038
ER  - 

@article{
author = "Pérez-Ramírez, Elisa and Cano-Gómez, Cristina and Llorente, Francisco and Vodica, Ani and Veljović, Ljubiša and Toklikishvilli, Natela and Sherifi, Kurtesh and Sghaier, Soufien and Omani, Amel and Kustura, Aida and Krstevski, Kiril and Karayel-Hacioglu, Ilke and Hagag, Naglaa Mohamed and Hage, Jeanne El and Davdyan, Hasmik and Bintarif, Mohd Saddam and Adžić, Bojan and Abouchoaib, Nabil and Jiménez-Clavero, Miguel Ángel and Fernández-Pinero, Jovita",
year = "2020",
abstract = "The increasing incidence of West Nile virus (WNV) in the Euro-Mediterranean area warrants the implementation of effective surveillance programs in animals. A crucial step in the fight against the disease is the evaluation of the capacity of the veterinary labs to accurately detect the infection in animal populations. In this context, the animal virology network of the MediLabSecure project organized an external quality assessment (EQA) to evaluate the WNV molecular and serological diagnostic capacities of beneficiary veterinary labs. Laboratories from 17 Mediterranean and Black Sea countries participated. The results of the triplex real time RT-PCR for simultaneous detection and differentiation of WNV lineage 1 (L1), lineage 2 (L2) and Usutu virus (USUV) were highly satisfactory, especially for L1 and L2, with detection rates of 97.9% and 100%, respectively. For USUV, 75% of the labs reported correct results. More limitations were observed for the generic detection of flaviviruses using conventional reverse-transcription polymerase chain reaction (RT-PCR), since only 46.1% reported correct results in the whole panel. As regards the serological panel, the results were excellent for the generic detection of WNV antibodies. More variability was observed for the specific detection of IgM antibodies with a higher percentage of incorrect results mainly in samples with low titers. This EQA provides a good overview of the WNV (and USUV) diagnostic performance of the involved veterinary labs and demonstrates that the implemented training program was successful in upgrading their diagnostic capacities.",
publisher = "MDPI AG",
journal = "Pathogens",
title = "Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions",
volume = "9",
number = "12",
pages = "1-20",
doi = "10.3390/pathogens9121038"
}

Pérez-Ramírez, E., Cano-Gómez, C., Llorente, F., Vodica, A., Veljović, L., Toklikishvilli, N., Sherifi, K., Sghaier, S., Omani, A., Kustura, A., Krstevski, K., Karayel-Hacioglu, I., Hagag, N. M., Hage, J. E., Davdyan, H., Bintarif, M. S., Adžić, B., Abouchoaib, N., Jiménez-Clavero, M. Á.,& Fernández-Pinero, J.. (2020). Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions. in Pathogens
MDPI AG., 9(12), 1-20.
https://doi.org/10.3390/pathogens9121038

Pérez-Ramírez E, Cano-Gómez C, Llorente F, Vodica A, Veljović L, Toklikishvilli N, Sherifi K, Sghaier S, Omani A, Kustura A, Krstevski K, Karayel-Hacioglu I, Hagag NM, Hage JE, Davdyan H, Bintarif MS, Adžić B, Abouchoaib N, Jiménez-Clavero MÁ, Fernández-Pinero J. Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions. in Pathogens. 2020;9(12):1-20.
doi:10.3390/pathogens9121038 .

Pérez-Ramírez, Elisa, Cano-Gómez, Cristina, Llorente, Francisco, Vodica, Ani, Veljović, Ljubiša, Toklikishvilli, Natela, Sherifi, Kurtesh, Sghaier, Soufien, Omani, Amel, Kustura, Aida, Krstevski, Kiril, Karayel-Hacioglu, Ilke, Hagag, Naglaa Mohamed, Hage, Jeanne El, Davdyan, Hasmik, Bintarif, Mohd Saddam, Adžić, Bojan, Abouchoaib, Nabil, Jiménez-Clavero, Miguel Ángel, Fernández-Pinero, Jovita, "Evaluation of west nile virus diagnostic capacities in veterinary laboratories of the mediterranean and black sea regions" in Pathogens, 9, no. 12 (2020):1-20,
https://doi.org/10.3390/pathogens9121038 . .