TY  - CONF
AU  - Galić, Ivan
AU  - Stančić, Ivan
AU  - Apić, Jelena
AU  - Maletić, Milan
AU  - Barna, Tomislav
AU  - Vračar, Vuk
AU  - Rodić, Stevan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3268
AB  - Reprodukcija svinja danas predstavlja osetljivo polje delovanja veterinarske službe koje
značajno utiče na profitabilnost svinjarske proizvodnje. Bez obzira na ovu činjenicu, neki
standardni pregledi razređenog semena nerastova kroz duži vremenski period predstavljaju
nezamenljiv način kontrole kvaliteta dobijenog ejakulata. Cito-morfološki pregled izvodi se u
svrhu procene učestalosti pojavljivanja abnormalno građenih spermatozoida. Citoplazmaske kapi
javljaju se na srednjem delu spermatozoida, a po mestu lokacije, dele se na proksimalne i distalne.
Distalne citoplazmatske kapi ukazuju na nezrelost spermatozoida, rano uvođenje mladih
nerastova u reproduktivnu eksploataciju, prekomerno iskorišćavanje priplodnjaka ili pak
posledično pojavljivanje ovakvih spermatozoida kao odgovor na ambijentalne oscilacije ili druge
zdravstvene probleme vezane za nerasta. Ovaj rad predstavlja istraživanje koje je sprovedeno na
nerastovima (n=30), durok i landras rasne pripadnosti. Eksperimentalna grupa životinja dobijala
je peroralno zajedno sa kompletnom smešom za ishranu, dodatak preparata sa antioksidativnim
potencijalom. Za potrebe bojenja spermatozoida korišćena je modifikovana metoda ‚‚u jednom
koraku” (Mortimer, 1994; Jovičin i sar., 2012). Primena dvofaktorske ANOVE za ponovljena
merenja pokazala je da nema statistički značajne razlike između kontrolne i eksperimentalne
grupe nerastova u nalazima citoplazmatskih kapi spermatozoida (%) (F(7, 112)= 1,65, p= 0,13) u
odabranim vremenskim tačkama. Iako nije zabeležena statistički značajna razlika, ovakav
rezultat, odnosno konstanta u broju ovakvih spermatozoida nerastova označava i ujednačenost
uslova pod kojim je ekseriment sproveden.
PB  - Požarevac : Sitograf RM
C3  - 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023
T1  - Citoplazmatske kapi spermatozoida nerastova – abnormalnost ili fiziološka pojava?
SP  - 174
EP  - 181
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3268
ER  - 

@conference{
author = "Galić, Ivan and Stančić, Ivan and Apić, Jelena and Maletić, Milan and Barna, Tomislav and Vračar, Vuk and Rodić, Stevan",
year = "2023",
abstract = "Reprodukcija svinja danas predstavlja osetljivo polje delovanja veterinarske službe koje
značajno utiče na profitabilnost svinjarske proizvodnje. Bez obzira na ovu činjenicu, neki
standardni pregledi razređenog semena nerastova kroz duži vremenski period predstavljaju
nezamenljiv način kontrole kvaliteta dobijenog ejakulata. Cito-morfološki pregled izvodi se u
svrhu procene učestalosti pojavljivanja abnormalno građenih spermatozoida. Citoplazmaske kapi
javljaju se na srednjem delu spermatozoida, a po mestu lokacije, dele se na proksimalne i distalne.
Distalne citoplazmatske kapi ukazuju na nezrelost spermatozoida, rano uvođenje mladih
nerastova u reproduktivnu eksploataciju, prekomerno iskorišćavanje priplodnjaka ili pak
posledično pojavljivanje ovakvih spermatozoida kao odgovor na ambijentalne oscilacije ili druge
zdravstvene probleme vezane za nerasta. Ovaj rad predstavlja istraživanje koje je sprovedeno na
nerastovima (n=30), durok i landras rasne pripadnosti. Eksperimentalna grupa životinja dobijala
je peroralno zajedno sa kompletnom smešom za ishranu, dodatak preparata sa antioksidativnim
potencijalom. Za potrebe bojenja spermatozoida korišćena je modifikovana metoda ‚‚u jednom
koraku” (Mortimer, 1994; Jovičin i sar., 2012). Primena dvofaktorske ANOVE za ponovljena
merenja pokazala je da nema statistički značajne razlike između kontrolne i eksperimentalne
grupe nerastova u nalazima citoplazmatskih kapi spermatozoida (%) (F(7, 112)= 1,65, p= 0,13) u
odabranim vremenskim tačkama. Iako nije zabeležena statistički značajna razlika, ovakav
rezultat, odnosno konstanta u broju ovakvih spermatozoida nerastova označava i ujednačenost
uslova pod kojim je ekseriment sproveden.",
publisher = "Požarevac : Sitograf RM",
journal = "20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023",
title = "Citoplazmatske kapi spermatozoida nerastova – abnormalnost ili fiziološka pojava?",
pages = "174-181",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3268"
}

Galić, I., Stančić, I., Apić, J., Maletić, M., Barna, T., Vračar, V.,& Rodić, S.. (2023). Citoplazmatske kapi spermatozoida nerastova – abnormalnost ili fiziološka pojava?. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023
Požarevac : Sitograf RM., 174-181.
https://hdl.handle.net/21.15107/rcub_veterinar_3268

Galić I, Stančić I, Apić J, Maletić M, Barna T, Vračar V, Rodić S. Citoplazmatske kapi spermatozoida nerastova – abnormalnost ili fiziološka pojava?. in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023. 2023;:174-181.
https://hdl.handle.net/21.15107/rcub_veterinar_3268 .

Galić, Ivan, Stančić, Ivan, Apić, Jelena, Maletić, Milan, Barna, Tomislav, Vračar, Vuk, Rodić, Stevan, "Citoplazmatske kapi spermatozoida nerastova – abnormalnost ili fiziološka pojava?" in 20. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 8 - 9. jun 2023 (2023):174-181,
https://hdl.handle.net/21.15107/rcub_veterinar_3268 .

TY  - CONF
AU  - Galić, Ivan
AU  - Stančić, Ivan
AU  - Maletić, Milan
AU  - Apić, Jelena
AU  - Barna, Tomislav
AU  - Rodić, Stevan
AU  - Ristevski, Dragan
PY  - 2023
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3214
AB  - Nizak antioksidativni kapacitet spermatozoida nerastova, često može biti i jedan
od glavnih razloga lošeg kvaliteta semena. Iz ove činjenice proizilazi i veoma velika
podložnost spermatozoida oksidativnom stresu i različitim štetnim efektima. Kada
proizvodnja reaktivnih vrsta kiseonika (ROS) nadmaši unutrašnju antioksidativnu
zaštitu, dolazi do probijanja sistema biološke odbrane, što za posledicu ima oštećenje
ćelije. Narušena redoks homeostaza ćelije, usled prekomernog stvaranja, ali i
neadekvatnog uklanjanja slobodnih radikala dovodi do oksidativnog stresa. Napad
reaktivnih vrsta kiseonika dovodi do smanjene pokretljivosti spermatozoida,
smanjene vitalnosti spermatozoida i povećanja morfoloških abnormalnosti
spermatozoida sa štetnim efektima po kapacitaciju spermatozoida i akrozomalnu
reakciju. Sa druge strane, niske koncentracije reaktivnih vrsta kiseonika igraju važnu
ulogu u fiziološkim procesima kao što su kapacitacija spermatozoida, reakcija
akrozoma i fertilizacija. Veća koncentracija antioksidativnih molekula u spermalnoj
plazmi, kompenzuje nizak antioksidativni kapacitet samih spermatozoida nerastova.
Sistem antioksidativne zaštite sastoji se od enzima koji uklanjaju slobodne radikale,
neenzimskih komponenti, specifičnih oksidoreduktaza i proteina. U membrani,
citoplazmi i organelama spermatozoida i spermalnoj plazmi, nalaze se enzimi
supreroksid dismutaza, glutation peroksidaza, glutation reduktaza, katalaza, među
kojima se superoksid dismutaza smatra najvažnijim antioksidativnim enzimom. Cilj
ovog rada je da na osnovu pregleda literature prikaže negativan efekat oksidativnog
stresa, ali i sistem antioksidativne zaštite koju poseduje sprmalna plazma nerastova.
PB  - Beograd : Srpsko veterinarsko društvo
C3  - 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
T1  - Negativan efekat oksidativnog stresa na plodnost priplodnih nerastova
SP  - 69
EP  - 77
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3214
ER  - 

@conference{
author = "Galić, Ivan and Stančić, Ivan and Maletić, Milan and Apić, Jelena and Barna, Tomislav and Rodić, Stevan and Ristevski, Dragan",
year = "2023",
abstract = "Nizak antioksidativni kapacitet spermatozoida nerastova, često može biti i jedan
od glavnih razloga lošeg kvaliteta semena. Iz ove činjenice proizilazi i veoma velika
podložnost spermatozoida oksidativnom stresu i različitim štetnim efektima. Kada
proizvodnja reaktivnih vrsta kiseonika (ROS) nadmaši unutrašnju antioksidativnu
zaštitu, dolazi do probijanja sistema biološke odbrane, što za posledicu ima oštećenje
ćelije. Narušena redoks homeostaza ćelije, usled prekomernog stvaranja, ali i
neadekvatnog uklanjanja slobodnih radikala dovodi do oksidativnog stresa. Napad
reaktivnih vrsta kiseonika dovodi do smanjene pokretljivosti spermatozoida,
smanjene vitalnosti spermatozoida i povećanja morfoloških abnormalnosti
spermatozoida sa štetnim efektima po kapacitaciju spermatozoida i akrozomalnu
reakciju. Sa druge strane, niske koncentracije reaktivnih vrsta kiseonika igraju važnu
ulogu u fiziološkim procesima kao što su kapacitacija spermatozoida, reakcija
akrozoma i fertilizacija. Veća koncentracija antioksidativnih molekula u spermalnoj
plazmi, kompenzuje nizak antioksidativni kapacitet samih spermatozoida nerastova.
Sistem antioksidativne zaštite sastoji se od enzima koji uklanjaju slobodne radikale,
neenzimskih komponenti, specifičnih oksidoreduktaza i proteina. U membrani,
citoplazmi i organelama spermatozoida i spermalnoj plazmi, nalaze se enzimi
supreroksid dismutaza, glutation peroksidaza, glutation reduktaza, katalaza, među
kojima se superoksid dismutaza smatra najvažnijim antioksidativnim enzimom. Cilj
ovog rada je da na osnovu pregleda literature prikaže negativan efekat oksidativnog
stresa, ali i sistem antioksidativne zaštite koju poseduje sprmalna plazma nerastova.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023",
title = "Negativan efekat oksidativnog stresa na plodnost priplodnih nerastova",
pages = "69-77",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3214"
}

Galić, I., Stančić, I., Maletić, M., Apić, J., Barna, T., Rodić, S.,& Ristevski, D.. (2023). Negativan efekat oksidativnog stresa na plodnost priplodnih nerastova. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023
Beograd : Srpsko veterinarsko društvo., 69-77.
https://hdl.handle.net/21.15107/rcub_veterinar_3214

Galić I, Stančić I, Maletić M, Apić J, Barna T, Rodić S, Ristevski D. Negativan efekat oksidativnog stresa na plodnost priplodnih nerastova. in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023. 2023;:69-77.
https://hdl.handle.net/21.15107/rcub_veterinar_3214 .

Galić, Ivan, Stančić, Ivan, Maletić, Milan, Apić, Jelena, Barna, Tomislav, Rodić, Stevan, Ristevski, Dragan, "Negativan efekat oksidativnog stresa na plodnost priplodnih nerastova" in 34. Savetovanje veterinara Srbije, Zlatibor, 07 - 10. septembar 2023 (2023):69-77,
https://hdl.handle.net/21.15107/rcub_veterinar_3214 .

TY  - JOUR
AU  - Barna, Tomislav
AU  - Apić, Jelena
AU  - Milovanović, Aleksandar
AU  - Maksimović, Nevena
AU  - Mašić, Aleksandar
AU  - Lazarević, Mmarina
AU  - Pavlović, Miloš
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2247
AB  - The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA), cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ± 100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%) in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters, as well as ratio of live sperm cells population with damaged acrosome. © 2021 TÜBİTAK.
PB  - Scientific Technical Research Council Turkey-Tubitak, Ankara
T2  - Turkish Journal of Veterinary and Animal Sciences
T1  - Quality and fertility of extended boar semen after prolonged transport
VL  - 45
IS  - 5
SP  - 920
EP  - 929
DO  - 10.3906/VET-2012-63
ER  - 

@article{
author = "Barna, Tomislav and Apić, Jelena and Milovanović, Aleksandar and Maksimović, Nevena and Mašić, Aleksandar and Lazarević, Mmarina and Pavlović, Miloš",
year = "2021",
abstract = "The aim of this study was to analyse semen quality parameters of fresh-extended porcine semen imported from Denmark through prolonged importing regulations and transport and to analyse fertility performance in sows inseminated four days post semen collection. Semen was analysed immediately upon the arrival (4 days post collection) using computer-assisted sperm analyses (CASA), cytomorphology and flow cytometry, and sows were inseminated later the same day. The mean sperm concentration was 1718.09 ± 100.11 × 106, with average of 1193.40 ± 90.58 × 106 motile cells (69%) and 756.04 ± 70.57 × 106 progressive motile spermatozoa (43.58%) in a dose, which was almost twice lower in value than producer certified-declared number of 2 billion of motile cells. Acrosome and sperm membrane integrity test indicated high percentage of total damaged acrosome. Mean values of semen samples analysed by sperm chromatin structure assay (SCSA) showed relatively high degree of chromatin damage. Membrane permeability test showed high percentage of sperm with damaged membrane. Prolonged transport negatively affected the sperm quality in terms of both motility and chromatin structure stability. Fertility of sows was affected by semen quality in terms of concentration and sperm motility parameters, as well as ratio of live sperm cells population with damaged acrosome. © 2021 TÜBİTAK.",
publisher = "Scientific Technical Research Council Turkey-Tubitak, Ankara",
journal = "Turkish Journal of Veterinary and Animal Sciences",
title = "Quality and fertility of extended boar semen after prolonged transport",
volume = "45",
number = "5",
pages = "920-929",
doi = "10.3906/VET-2012-63"
}

Barna, T., Apić, J., Milovanović, A., Maksimović, N., Mašić, A., Lazarević, M.,& Pavlović, M.. (2021). Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences
Scientific Technical Research Council Turkey-Tubitak, Ankara., 45(5), 920-929.
https://doi.org/10.3906/VET-2012-63

Barna T, Apić J, Milovanović A, Maksimović N, Mašić A, Lazarević M, Pavlović M. Quality and fertility of extended boar semen after prolonged transport. in Turkish Journal of Veterinary and Animal Sciences. 2021;45(5):920-929.
doi:10.3906/VET-2012-63 .

Barna, Tomislav, Apić, Jelena, Milovanović, Aleksandar, Maksimović, Nevena, Mašić, Aleksandar, Lazarević, Mmarina, Pavlović, Miloš, "Quality and fertility of extended boar semen after prolonged transport" in Turkish Journal of Veterinary and Animal Sciences, 45, no. 5 (2021):920-929,
https://doi.org/10.3906/VET-2012-63 . .

TY  - GEN
AU  - Apić, Jelena
AU  - Galić, Ivan
AU  - Stančić, Ivan
AU  - Barna, Tomislav
AU  - Vakanjac, Slobodanka
AU  - Milovanović, Aleksandar
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3160
AB  - Današnje savremeno svinjarstvo ne može da se zamisli bez primene biotehnološke
metode veštačkog osemenjavanja. Efikasno reproduktivno iskorišćavanje, genetski
superiornih nerastova, zahteva formiranje maksimalnog broja inseminacionih doza po
ejakulatu, odnosno po nerastu godišnje. Rezultati brojnih istraživanja dokazuju da bi
proteini spermalne plazme mogli imati značajan uticaj na variranje stepena progresivne
pokretlјivosti spermatozoida i održavanje fertilizacionog kapaciteta razređene
sperme pojedinih nerastova. U naše istraživanje je bilo uključeno 15 nerastova rase
švedski landras (5) veliki jorkšir (5) i durok (5) približne starosti od 18 do 24 meseca,
u punoj proizvodnoj ekploataciji. Od svakog nerasta su uzeta po tri ejakulata (ukupno
45 ejakulata), u razmaku od mesec dana, u hladnijem delu godine (oktobar-novembar-
decembar). Kod svih nerastova je utvrđivan procenat ukupnih proteina u spermalnoj
plazmi. Na osnovu dobijenih rezulata sadržaja ukupnih proteina u spermalnoj plazmi,
ispitivani nerastovi su podeljeni u dve grupe, grupa (N)-(niski): nerastovi sa niskim
sadržajem proteina (≤ 2,0) i grupa (V)-(visoki): nerastovi sa visokim sadržajem proteina
(≥ 2,1). Poređenjem parametara kvaliteta razređenog semena između ove dve grupe,
utvrđeno je da su prosečne vrednosti svih ispitivanih parametara fertilizacionog potencijala
razređenog semena bile statistički značajno veće u grupi V u odnosu na grupu
N, (p<0,01). Tako su prosečne vrednosti za ukupan broj spermatozoida u dozi iznosile
6,293×109 (V) i 3,595×109 (N), ukupan broj pokretnih spermatozoida u dozi 4,771×109
(V) i 1,325×109(N), procenat ukupno pokretnih spermatozoida 74,20 (V) i 36,26 (N),
ukupan broj progresivno pokretnih spermatozoida 2,982×109 (V) i 731,47×106(N), a
procenat progresivno pokretnih spermatozoida je iznosio 47,90 (V) i 21,42 (N). Nisu
ustanovljene statistički značajne (p>0,05) razlike u prosečom sadržaju proteina u spermalnoj
plazmi, između ispitivanih rasa nerastova. Ova vrednost je iznosila 2,43 procenta
kod rase landras, 2,45 kod rase veliki jorkšir i 1,83 kod rase durok. Prosečna vrednost
sadržaja proteina u spermalnoj plazmi nije značajno (p>0,05) varirala ni u zavisnosti od starosti ispitivanih nerastova. Navedene činjenice, ukazuju da sadržaj proteina u
spermalnoj plazmi, može biti efikasan marker fertilizacionog potencijala nerastova, na
osnovu koga se oni mogu selekcionisati za upotrebu u veštačkom osemenjavanju.
PB  - Beograd : Srpsko veterinarsko društvo
T2  - 32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021
T1  - Proteini spermalne plazme nerastova kao genetski markeri kvaliteta semena
SP  - 92
EP  - 103
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3160
ER  - 

@misc{
author = "Apić, Jelena and Galić, Ivan and Stančić, Ivan and Barna, Tomislav and Vakanjac, Slobodanka and Milovanović, Aleksandar",
year = "2021",
abstract = "Današnje savremeno svinjarstvo ne može da se zamisli bez primene biotehnološke
metode veštačkog osemenjavanja. Efikasno reproduktivno iskorišćavanje, genetski
superiornih nerastova, zahteva formiranje maksimalnog broja inseminacionih doza po
ejakulatu, odnosno po nerastu godišnje. Rezultati brojnih istraživanja dokazuju da bi
proteini spermalne plazme mogli imati značajan uticaj na variranje stepena progresivne
pokretlјivosti spermatozoida i održavanje fertilizacionog kapaciteta razređene
sperme pojedinih nerastova. U naše istraživanje je bilo uključeno 15 nerastova rase
švedski landras (5) veliki jorkšir (5) i durok (5) približne starosti od 18 do 24 meseca,
u punoj proizvodnoj ekploataciji. Od svakog nerasta su uzeta po tri ejakulata (ukupno
45 ejakulata), u razmaku od mesec dana, u hladnijem delu godine (oktobar-novembar-
decembar). Kod svih nerastova je utvrđivan procenat ukupnih proteina u spermalnoj
plazmi. Na osnovu dobijenih rezulata sadržaja ukupnih proteina u spermalnoj plazmi,
ispitivani nerastovi su podeljeni u dve grupe, grupa (N)-(niski): nerastovi sa niskim
sadržajem proteina (≤ 2,0) i grupa (V)-(visoki): nerastovi sa visokim sadržajem proteina
(≥ 2,1). Poređenjem parametara kvaliteta razređenog semena između ove dve grupe,
utvrđeno je da su prosečne vrednosti svih ispitivanih parametara fertilizacionog potencijala
razređenog semena bile statistički značajno veće u grupi V u odnosu na grupu
N, (p<0,01). Tako su prosečne vrednosti za ukupan broj spermatozoida u dozi iznosile
6,293×109 (V) i 3,595×109 (N), ukupan broj pokretnih spermatozoida u dozi 4,771×109
(V) i 1,325×109(N), procenat ukupno pokretnih spermatozoida 74,20 (V) i 36,26 (N),
ukupan broj progresivno pokretnih spermatozoida 2,982×109 (V) i 731,47×106(N), a
procenat progresivno pokretnih spermatozoida je iznosio 47,90 (V) i 21,42 (N). Nisu
ustanovljene statistički značajne (p>0,05) razlike u prosečom sadržaju proteina u spermalnoj
plazmi, između ispitivanih rasa nerastova. Ova vrednost je iznosila 2,43 procenta
kod rase landras, 2,45 kod rase veliki jorkšir i 1,83 kod rase durok. Prosečna vrednost
sadržaja proteina u spermalnoj plazmi nije značajno (p>0,05) varirala ni u zavisnosti od starosti ispitivanih nerastova. Navedene činjenice, ukazuju da sadržaj proteina u
spermalnoj plazmi, može biti efikasan marker fertilizacionog potencijala nerastova, na
osnovu koga se oni mogu selekcionisati za upotrebu u veštačkom osemenjavanju.",
publisher = "Beograd : Srpsko veterinarsko društvo",
journal = "32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021",
title = "Proteini spermalne plazme nerastova kao genetski markeri kvaliteta semena",
pages = "92-103",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3160"
}

Apić, J., Galić, I., Stančić, I., Barna, T., Vakanjac, S.,& Milovanović, A.. (2021). Proteini spermalne plazme nerastova kao genetski markeri kvaliteta semena. in 32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021
Beograd : Srpsko veterinarsko društvo., 92-103.
https://hdl.handle.net/21.15107/rcub_veterinar_3160

Apić J, Galić I, Stančić I, Barna T, Vakanjac S, Milovanović A. Proteini spermalne plazme nerastova kao genetski markeri kvaliteta semena. in 32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021. 2021;:92-103.
https://hdl.handle.net/21.15107/rcub_veterinar_3160 .

Apić, Jelena, Galić, Ivan, Stančić, Ivan, Barna, Tomislav, Vakanjac, Slobodanka, Milovanović, Aleksandar, "Proteini spermalne plazme nerastova kao genetski markeri kvaliteta semena" in 32. Savetovanje veterinara Srbije, Zlatibor, 9 – 12. septembar 2021 (2021):92-103,
https://hdl.handle.net/21.15107/rcub_veterinar_3160 .

TY  - CONF
AU  - Milovanoviić, Aleksandar
AU  - Barna, Tomislav
AU  - Apić, Jelena
AU  - Stojanov, Igor
AU  - Lazarević, Miodrag
AU  - Maksimović, Nevena
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3426
AB  - U ovom izlaganju će biti prikazan uticaj zračenja semena nerastova crvenim delom
svetlosnog spektra neposredno pre osemenjavanja na veličinu legla krmača. Korišćene su LED
lampe ugrađene u klima boks koje emituju spektar crvene boje, talasne dužine od 620 do 630
nm, sa režimom rada od 10 minuta svetlosti, 10 minuta odmora i 10 minuta dodatnog zračenja.
Osemenjavanja su sprovedena u periodu od aprila do maja 2021. godine, na farmi od 1
200 priplodnih krmača. U kontrolnoj grupi su bile 174 krmače, sa prosečnih 4,36 prašenja, a u
oglednoj 64, sa paritetom od 4,14. Nazimice nisu bile uključene u statističku obradu.
U oglednoj grupi je registrovan veći broj živorođene prasadi za 0,87 (14,05 prema
13,17), ali bez statističke značajnosti, dok je u istoj grupi, zabeležen znatno manji broj avitalne
i mrtvorođene prasadi (3,55 prema 4,68; p<0,05). Broj mumificirane prasadi u leglima je bio
identičan (0,45).
Zračenje semena crvenim svetlosnim zracima, neposredno pre osemenjavanja imalo je
pozitivan efekat na broj živorođene prasadi u leglu, ali samo u numeričkoj vrednosti, dok je
jasna statistička razlika uočena u pogledu njihove vitalnosti.
Ovaj ogled je u toku i očekuje se analiza znatno većeg broja prašenja, a time i jasniji
uvid u efekte ovakvog tretmana semena na oprasivost.
AB  - In this presentation, the influence of irradiation of boar semen by red light spectrum
immediately before insemination on the litter size in sows will be presented. LED lamps that
emit a red color spectrum with a wavelength of 620-630 nm built into the air conditioning box
were used. A mode of operation was: 10 minutes of light, 10 minutes of rest and 10 minutes of
additional irradiation.
Inseminations were carried out in the period from April to May 2021, on a farm of 1,200
breeding sows. Control group consisted of 174 sows with an average of 4.36 parities, and
experimental group of 64 sows, with a parity of 4.14. Gillts were excluded from the statistics.
In the experimental group, a higher number of live-born piglets was observed by 0.87
(14.05 vs. 13.17) but with no statistical significance, while in the same group, a significantly
lower number of avital and stillborn piglets was also observed (3.55 vs. 4.68; p <0.05 ). The
number of mummified piglets in the litters was identical (0.45 piglets) for both groups.
Semen irradiation with red light rays immediately before insemination had a positive
effect on the number of live-born piglets in the litter, but only in numerical value, while a clear
statistical difference was observed in terms of vitality of piglets in litters.
This trial is in progress and a significantly larger number of farrowings and a clearer
insight into the efficiency of such semen treatment on the fertility is expected.
PB  - Požarevac : Sitograf RM : Veterinarski specijalistički institut
C3  - 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021
T1  - Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača
T1  - Influence of red light spectrum irradiation of boar semen on litter size in sows
SP  - 71
EP  - 72
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3426
ER  - 

@conference{
author = "Milovanoviić, Aleksandar and Barna, Tomislav and Apić, Jelena and Stojanov, Igor and Lazarević, Miodrag and Maksimović, Nevena",
year = "2021",
abstract = "U ovom izlaganju će biti prikazan uticaj zračenja semena nerastova crvenim delom
svetlosnog spektra neposredno pre osemenjavanja na veličinu legla krmača. Korišćene su LED
lampe ugrađene u klima boks koje emituju spektar crvene boje, talasne dužine od 620 do 630
nm, sa režimom rada od 10 minuta svetlosti, 10 minuta odmora i 10 minuta dodatnog zračenja.
Osemenjavanja su sprovedena u periodu od aprila do maja 2021. godine, na farmi od 1
200 priplodnih krmača. U kontrolnoj grupi su bile 174 krmače, sa prosečnih 4,36 prašenja, a u
oglednoj 64, sa paritetom od 4,14. Nazimice nisu bile uključene u statističku obradu.
U oglednoj grupi je registrovan veći broj živorođene prasadi za 0,87 (14,05 prema
13,17), ali bez statističke značajnosti, dok je u istoj grupi, zabeležen znatno manji broj avitalne
i mrtvorođene prasadi (3,55 prema 4,68; p<0,05). Broj mumificirane prasadi u leglima je bio
identičan (0,45).
Zračenje semena crvenim svetlosnim zracima, neposredno pre osemenjavanja imalo je
pozitivan efekat na broj živorođene prasadi u leglu, ali samo u numeričkoj vrednosti, dok je
jasna statistička razlika uočena u pogledu njihove vitalnosti.
Ovaj ogled je u toku i očekuje se analiza znatno većeg broja prašenja, a time i jasniji
uvid u efekte ovakvog tretmana semena na oprasivost., In this presentation, the influence of irradiation of boar semen by red light spectrum
immediately before insemination on the litter size in sows will be presented. LED lamps that
emit a red color spectrum with a wavelength of 620-630 nm built into the air conditioning box
were used. A mode of operation was: 10 minutes of light, 10 minutes of rest and 10 minutes of
additional irradiation.
Inseminations were carried out in the period from April to May 2021, on a farm of 1,200
breeding sows. Control group consisted of 174 sows with an average of 4.36 parities, and
experimental group of 64 sows, with a parity of 4.14. Gillts were excluded from the statistics.
In the experimental group, a higher number of live-born piglets was observed by 0.87
(14.05 vs. 13.17) but with no statistical significance, while in the same group, a significantly
lower number of avital and stillborn piglets was also observed (3.55 vs. 4.68; p <0.05 ). The
number of mummified piglets in the litters was identical (0.45 piglets) for both groups.
Semen irradiation with red light rays immediately before insemination had a positive
effect on the number of live-born piglets in the litter, but only in numerical value, while a clear
statistical difference was observed in terms of vitality of piglets in litters.
This trial is in progress and a significantly larger number of farrowings and a clearer
insight into the efficiency of such semen treatment on the fertility is expected.",
publisher = "Požarevac : Sitograf RM : Veterinarski specijalistički institut",
journal = "18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021",
title = "Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača, Influence of red light spectrum irradiation of boar semen on litter size in sows",
pages = "71-72",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3426"
}

Milovanoviić, A., Barna, T., Apić, J., Stojanov, I., Lazarević, M.,& Maksimović, N.. (2021). Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača. in 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021
Požarevac : Sitograf RM : Veterinarski specijalistički institut., 71-72.
https://hdl.handle.net/21.15107/rcub_veterinar_3426

Milovanoviić A, Barna T, Apić J, Stojanov I, Lazarević M, Maksimović N. Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača. in 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021. 2021;:71-72.
https://hdl.handle.net/21.15107/rcub_veterinar_3426 .

Milovanoviić, Aleksandar, Barna, Tomislav, Apić, Jelena, Stojanov, Igor, Lazarević, Miodrag, Maksimović, Nevena, "Uticaj zračenja semena nerastova crvenim delom svetlosnog spektra na veličinu legla krmača" in 18. Simpozijum Zdravstvena zaštita, selekcija i reprodukcija svinja, Beograd, 15 - 16 oktobar, 2021 (2021):71-72,
https://hdl.handle.net/21.15107/rcub_veterinar_3426 .

TY  - JOUR
AU  - Stojanov, Igor
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Prodanov-Radulović, Jasna
AU  - Apić, Jelena
AU  - Stojanović, Dragica
AU  - Maksimović, Nevena
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2385
AB  - The aim of the study was to determine whether the bacteria from the environment and from the mucous membrane of the boar prepuce have antimicrobial resistance and whether the result obtained is similar/same to the bacteria that can be found in native boar semen. The study addresses the problem of the presence of primarily resistant bacterial strains in the boar sperm, which, due to their reduced sensitivity, cannot be suppressed by antibiotics used in the semen dilution agent, as well as to emphasize the importance of microbiological monitoring of the boar mucous membranes and ambient surfaces before and during their exploitation. Such an examination could contribute to the interchangeable design of the dilution agent for the boar semen relative to the antibiotic content.Resistant strains of bacteria from prepuce swabs and swabs taken from the facility, as well as from native boar semen were isolated. The presence of these bacteria affected the quality of the semen. In conclusion, it should be pointed out that bacterial monitoring of the prepuce and surface of the facility can indicate possible problems related to the quality of semen, and that the design of the dilution agent for boar semen should be adjusted to the established resistance of isolated bacteria.
AB  - Cilj ispitivanja je da se utvrdi da li bakterije iz okoline i sa sluznici prepucijma poseduju antimikrobnu rezistenciju i da li je dobijeni nalaz sličan/isti sa bakterijama koje se mogu naći u nativnom semenu nerasta. Ispitivanje treba da odgovori na problem prisustva, pre svega rezistentnih bakterijskih sojeva u spermi nerasta koji zbog svoje smanjene osteljivosti ne mogu biti suprimirani antibioticima, koji se nalaze u razređivaču za seme, kao i da istakne značaj mikrobiološkog monitoringa sluznice nerasta i ambijentalnih površina pre i tokom njihove eksploatacije. Ovakvo ispitivanje bi moglo da doprinese izmenjivom dizajniranju razređivača za seme nerasta u odnosu na sadržaj antibiotika. Izolovani su rezistentni sojevi bakterija iz briseva prepucijuma i briseva uzetih iz objekta kao i iz nativnog semena nerasta. Prisustvo ovih bakterija uticalo na kvalitet semena. Kao zaključak treba istaći da bakterijski monitoring prepucijuma i površina u objektu može ukazati na moguće probleme vezane za kvalitet semena nerasta kao i da se dizajniranje razređivača za seme nerasta prilagodi utvrđenoj rezistenciji izolovanih bakterija.
PB  - Faculty of Veterinary Medicine
PB  - Sciendo
T2  - Acta Veterinaria-Beograd
T1  - Antimicrobial resistance as a problem for the quality of Boar Semen
T1  - Antimikrobna rezistencija kao problem kvaliteta semena nerasta
VL  - 70
IS  - 1
SP  - 136
EP  - 146
DO  - 10.2478/acve-2020-0010
ER  - 

@article{
author = "Stojanov, Igor and Milovanović, Aleksandar and Barna, Tomislav and Prodanov-Radulović, Jasna and Apić, Jelena and Stojanović, Dragica and Maksimović, Nevena",
year = "2020",
abstract = "The aim of the study was to determine whether the bacteria from the environment and from the mucous membrane of the boar prepuce have antimicrobial resistance and whether the result obtained is similar/same to the bacteria that can be found in native boar semen. The study addresses the problem of the presence of primarily resistant bacterial strains in the boar sperm, which, due to their reduced sensitivity, cannot be suppressed by antibiotics used in the semen dilution agent, as well as to emphasize the importance of microbiological monitoring of the boar mucous membranes and ambient surfaces before and during their exploitation. Such an examination could contribute to the interchangeable design of the dilution agent for the boar semen relative to the antibiotic content.Resistant strains of bacteria from prepuce swabs and swabs taken from the facility, as well as from native boar semen were isolated. The presence of these bacteria affected the quality of the semen. In conclusion, it should be pointed out that bacterial monitoring of the prepuce and surface of the facility can indicate possible problems related to the quality of semen, and that the design of the dilution agent for boar semen should be adjusted to the established resistance of isolated bacteria., Cilj ispitivanja je da se utvrdi da li bakterije iz okoline i sa sluznici prepucijma poseduju antimikrobnu rezistenciju i da li je dobijeni nalaz sličan/isti sa bakterijama koje se mogu naći u nativnom semenu nerasta. Ispitivanje treba da odgovori na problem prisustva, pre svega rezistentnih bakterijskih sojeva u spermi nerasta koji zbog svoje smanjene osteljivosti ne mogu biti suprimirani antibioticima, koji se nalaze u razređivaču za seme, kao i da istakne značaj mikrobiološkog monitoringa sluznice nerasta i ambijentalnih površina pre i tokom njihove eksploatacije. Ovakvo ispitivanje bi moglo da doprinese izmenjivom dizajniranju razređivača za seme nerasta u odnosu na sadržaj antibiotika. Izolovani su rezistentni sojevi bakterija iz briseva prepucijuma i briseva uzetih iz objekta kao i iz nativnog semena nerasta. Prisustvo ovih bakterija uticalo na kvalitet semena. Kao zaključak treba istaći da bakterijski monitoring prepucijuma i površina u objektu može ukazati na moguće probleme vezane za kvalitet semena nerasta kao i da se dizajniranje razređivača za seme nerasta prilagodi utvrđenoj rezistenciji izolovanih bakterija.",
publisher = "Faculty of Veterinary Medicine, Sciendo",
journal = "Acta Veterinaria-Beograd",
title = "Antimicrobial resistance as a problem for the quality of Boar Semen, Antimikrobna rezistencija kao problem kvaliteta semena nerasta",
volume = "70",
number = "1",
pages = "136-146",
doi = "10.2478/acve-2020-0010"
}

Stojanov, I., Milovanović, A., Barna, T., Prodanov-Radulović, J., Apić, J., Stojanović, D.,& Maksimović, N.. (2020). Antimicrobial resistance as a problem for the quality of Boar Semen. in Acta Veterinaria-Beograd
Faculty of Veterinary Medicine., 70(1), 136-146.
https://doi.org/10.2478/acve-2020-0010

Stojanov I, Milovanović A, Barna T, Prodanov-Radulović J, Apić J, Stojanović D, Maksimović N. Antimicrobial resistance as a problem for the quality of Boar Semen. in Acta Veterinaria-Beograd. 2020;70(1):136-146.
doi:10.2478/acve-2020-0010 .

Stojanov, Igor, Milovanović, Aleksandar, Barna, Tomislav, Prodanov-Radulović, Jasna, Apić, Jelena, Stojanović, Dragica, Maksimović, Nevena, "Antimicrobial resistance as a problem for the quality of Boar Semen" in Acta Veterinaria-Beograd, 70, no. 1 (2020):136-146,
https://doi.org/10.2478/acve-2020-0010 . .

TY  - CONF
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Apić, Jelena
AU  - Stojanov, Igor
AU  - Lazarević, Miodrag
AU  - Vasiljević, Teodora
AU  - Antić, Danko
AU  - Mašić, Aleksandar
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/3435
PB  - Požarevac : Veterinarski specijalistički institut
C3  - 17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
T1  - Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati
SP  - 142
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_3435
ER  - 

@conference{
author = "Milovanović, Aleksandar and Barna, Tomislav and Apić, Jelena and Stojanov, Igor and Lazarević, Miodrag and Vasiljević, Teodora and Antić, Danko and Mašić, Aleksandar",
year = "2019",
publisher = "Požarevac : Veterinarski specijalistički institut",
journal = "17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019",
title = "Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati",
pages = "142-143",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_3435"
}

Milovanović, A., Barna, T., Apić, J., Stojanov, I., Lazarević, M., Vasiljević, T., Antić, D.,& Mašić, A.. (2019). Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati. in 17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019
Požarevac : Veterinarski specijalistički institut., 142-143.
https://hdl.handle.net/21.15107/rcub_veterinar_3435

Milovanović A, Barna T, Apić J, Stojanov I, Lazarević M, Vasiljević T, Antić D, Mašić A. Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati. in 17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019. 2019;:142-143.
https://hdl.handle.net/21.15107/rcub_veterinar_3435 .

Milovanović, Aleksandar, Barna, Tomislav, Apić, Jelena, Stojanov, Igor, Lazarević, Miodrag, Vasiljević, Teodora, Antić, Danko, Mašić, Aleksandar, "Primena imunostimulatora u korekciji plodnosti krmača i odgoju prasadi u ranoj fazi - preliminarni rezultati" in 17. Savetovanje Zdravstvena zaštita, selekcija i reprodukcija svinja, Srebrno jezero, 30. maj - 1. jun, 2019 (2019):142-143,
https://hdl.handle.net/21.15107/rcub_veterinar_3435 .

TY  - JOUR
AU  - Suluburić, Adam
AU  - Milanović, Svetlana
AU  - Vranješ-Đurić, Sanja
AU  - Jovanović, Ivan B.
AU  - Barna, Tomislav
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Szenci, Otto
AU  - Gvozdić, Dragan
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1486
AB  - Early embryonic development may be negatively affected by insufficient progesterone (P4) production. Therefore, the aim of our study was to increase P4 by gonadotropin-releasing hormone (GnRH) and/or human chorionic gonadotropin (hCG) treatments after inducing oestrus by prostaglandin (PG) treatment. Lactating Simmental dairy cows (n = 110), between 1 to 5 lactations, with an average milk production of 6,500 l/305 days, at 40-80 days postpartum were used and grouped as follows: (1) PG + GnRH treatment at AI (GnRH group), (2) PG + hCG treatment at day 7 after AI (hCG group), (3) PG + GnRH at AI + hCG treatment at day 7 after AI (GnRH/hCG group), and (4) spontaneous oestrus (C: control group). All animals were double inseminated (at the time of oestrus detection and 12 +/- 2 h thereafter). Blood serum and milk samples were collected at the day of observed oestrus (day 0), and 14, 21 and 28 days after AI. Serum P4 was determined using a commercial radioimmunoassay (RIA) test (INEP, Zemun), and milk P4 was determined using enzyme-linked immunoassay (ELISA) test (NIV Novi Sad). Pregnancy status was confirmed by ultrasonography between days 28 and 35 after AI. Differences of serum or milk P4 medians, pregnancy (and calving) rate were determined using Dunns Multiple Comparison Tests and Z test, respectively. Serum P4 medians were significantly higher at days 14, 21 and 28 after AI in the hCG-treated animals, indicating increased luteal activity, with a similar tendency in whole milk P4 values. Treatment with hCG during the early luteal phase significantly contributed to the maintenance of gestation at days 28-35 after AI, and also increased the calving rate in Simmental dairy cows.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Veterinaria Hungarica
T1  - Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase
VL  - 65
IS  - 3
SP  - 446
EP  - 458
DO  - 10.1556/004.2017.042
ER  - 

@article{
author = "Suluburić, Adam and Milanović, Svetlana and Vranješ-Đurić, Sanja and Jovanović, Ivan B. and Barna, Tomislav and Stojić, Milica and Fratrić, Natalija and Szenci, Otto and Gvozdić, Dragan",
year = "2017",
abstract = "Early embryonic development may be negatively affected by insufficient progesterone (P4) production. Therefore, the aim of our study was to increase P4 by gonadotropin-releasing hormone (GnRH) and/or human chorionic gonadotropin (hCG) treatments after inducing oestrus by prostaglandin (PG) treatment. Lactating Simmental dairy cows (n = 110), between 1 to 5 lactations, with an average milk production of 6,500 l/305 days, at 40-80 days postpartum were used and grouped as follows: (1) PG + GnRH treatment at AI (GnRH group), (2) PG + hCG treatment at day 7 after AI (hCG group), (3) PG + GnRH at AI + hCG treatment at day 7 after AI (GnRH/hCG group), and (4) spontaneous oestrus (C: control group). All animals were double inseminated (at the time of oestrus detection and 12 +/- 2 h thereafter). Blood serum and milk samples were collected at the day of observed oestrus (day 0), and 14, 21 and 28 days after AI. Serum P4 was determined using a commercial radioimmunoassay (RIA) test (INEP, Zemun), and milk P4 was determined using enzyme-linked immunoassay (ELISA) test (NIV Novi Sad). Pregnancy status was confirmed by ultrasonography between days 28 and 35 after AI. Differences of serum or milk P4 medians, pregnancy (and calving) rate were determined using Dunns Multiple Comparison Tests and Z test, respectively. Serum P4 medians were significantly higher at days 14, 21 and 28 after AI in the hCG-treated animals, indicating increased luteal activity, with a similar tendency in whole milk P4 values. Treatment with hCG during the early luteal phase significantly contributed to the maintenance of gestation at days 28-35 after AI, and also increased the calving rate in Simmental dairy cows.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Veterinaria Hungarica",
title = "Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase",
volume = "65",
number = "3",
pages = "446-458",
doi = "10.1556/004.2017.042"
}

Suluburić, A., Milanović, S., Vranješ-Đurić, S., Jovanović, I. B., Barna, T., Stojić, M., Fratrić, N., Szenci, O.,& Gvozdić, D.. (2017). Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase. in Acta Veterinaria Hungarica
Akademiai Kiado Rt, Budapest., 65(3), 446-458.
https://doi.org/10.1556/004.2017.042

Suluburić A, Milanović S, Vranješ-Đurić S, Jovanović IB, Barna T, Stojić M, Fratrić N, Szenci O, Gvozdić D. Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase. in Acta Veterinaria Hungarica. 2017;65(3):446-458.
doi:10.1556/004.2017.042 .

Suluburić, Adam, Milanović, Svetlana, Vranješ-Đurić, Sanja, Jovanović, Ivan B., Barna, Tomislav, Stojić, Milica, Fratrić, Natalija, Szenci, Otto, Gvozdić, Dragan, "Progesterone concentration, pregnancy and calving rate in simmental dairy cows after oestrus synchronisation and HCG treatment during the early luteal phase" in Acta Veterinaria Hungarica, 65, no. 3 (2017):446-458,
https://doi.org/10.1556/004.2017.042 . .

TY  - JOUR
AU  - Apić, Jelena
AU  - Stančić, Ivan
AU  - Vakanjac, Slobodanka
AU  - Radović, I.
AU  - Milovanović, A.
AU  - Barna, Tomislav
AU  - Maletić, Milan
PY  - 2016
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1333
AB  - The aim of the present study was to investigate the influence of the protein content of seminal plasma on the motility, viability and acrosome integrity of spermatozoa in extended semen stored for 3 days. A total of 32 semen samples (from four boars) with high (4 mg/ml) and 32 semen samples (from four boars) with low (2 mg/ml) protein content were investigated. The semen samples were diluted by BTS at a ratio of 1:4, and stored for 72 h at 17oC. The percentages of live sperm (LS), live sperm with damaged acrosome (LDA) and total sperm with damaged acrosome (TDA) were detected by flow cytometry. Sperm progressive motility (PM) was detected using CASA. After 72 h of storage, the percentage of LS and PM was significantly (P  lt  0.01) higher, and the LDA and TDA were significantly (P  lt  0.01) lower in samples with high protein content than in the samples with low protein content (LS = 66 vs. 44%, PM = 64 vs. 48%, LDA = 15 vs. 21% and TDA = 29 vs. 45%, respectively). When comparing the difference between 0 and 72 h of storage, the percentage decrease in LS and PM, while increase in LDA and TDA were significantly higher in the samples with low (LS: 75 to 44%; PM: 68 to 48%; LDA: 11 to 21% and TDA: 23 to 45%) than in the samples with high protein content (LS: 78 to 66%; PM: 70 to 64%; LDA: 9 to 15% and TDA: 17 to 29%). We concluded that protein content in seminal plasma has a significant influence on progressive motility, viability and acrosome integrity in diluted semen stored for 3 days.
PB  - Colegio Brasileiro de Reproducao Animal
T2  - Animal Reproduction
T1  - Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days
VL  - 13
IS  - 1
SP  - 36
EP  - 41
DO  - 10.4322/1984-3143-AR792
ER  - 

@article{
author = "Apić, Jelena and Stančić, Ivan and Vakanjac, Slobodanka and Radović, I. and Milovanović, A. and Barna, Tomislav and Maletić, Milan",
year = "2016",
abstract = "The aim of the present study was to investigate the influence of the protein content of seminal plasma on the motility, viability and acrosome integrity of spermatozoa in extended semen stored for 3 days. A total of 32 semen samples (from four boars) with high (4 mg/ml) and 32 semen samples (from four boars) with low (2 mg/ml) protein content were investigated. The semen samples were diluted by BTS at a ratio of 1:4, and stored for 72 h at 17oC. The percentages of live sperm (LS), live sperm with damaged acrosome (LDA) and total sperm with damaged acrosome (TDA) were detected by flow cytometry. Sperm progressive motility (PM) was detected using CASA. After 72 h of storage, the percentage of LS and PM was significantly (P  lt  0.01) higher, and the LDA and TDA were significantly (P  lt  0.01) lower in samples with high protein content than in the samples with low protein content (LS = 66 vs. 44%, PM = 64 vs. 48%, LDA = 15 vs. 21% and TDA = 29 vs. 45%, respectively). When comparing the difference between 0 and 72 h of storage, the percentage decrease in LS and PM, while increase in LDA and TDA were significantly higher in the samples with low (LS: 75 to 44%; PM: 68 to 48%; LDA: 11 to 21% and TDA: 23 to 45%) than in the samples with high protein content (LS: 78 to 66%; PM: 70 to 64%; LDA: 9 to 15% and TDA: 17 to 29%). We concluded that protein content in seminal plasma has a significant influence on progressive motility, viability and acrosome integrity in diluted semen stored for 3 days.",
publisher = "Colegio Brasileiro de Reproducao Animal",
journal = "Animal Reproduction",
title = "Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days",
volume = "13",
number = "1",
pages = "36-41",
doi = "10.4322/1984-3143-AR792"
}

Apić, J., Stančić, I., Vakanjac, S., Radović, I., Milovanović, A., Barna, T.,& Maletić, M.. (2016). Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days. in Animal Reproduction
Colegio Brasileiro de Reproducao Animal., 13(1), 36-41.
https://doi.org/10.4322/1984-3143-AR792

Apić J, Stančić I, Vakanjac S, Radović I, Milovanović A, Barna T, Maletić M. Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days. in Animal Reproduction. 2016;13(1):36-41.
doi:10.4322/1984-3143-AR792 .

Apić, Jelena, Stančić, Ivan, Vakanjac, Slobodanka, Radović, I., Milovanović, A., Barna, Tomislav, Maletić, Milan, "Influence of the protein content of boar seminal plasma on spermatozoa viability, motility and acrosome integrity in diluted semen stored for 3 days" in Animal Reproduction, 13, no. 1 (2016):36-41,
https://doi.org/10.4322/1984-3143-AR792 . .

TY  - JOUR
AU  - Apić, Jelena
AU  - Vakanjac, Slobodanka
AU  - Radović, Ivan
AU  - Jotanović, Stoja
AU  - Živkov-Baloš, Milica
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Stanković, Branislav
AU  - Jajić, Igor
AU  - Maletić, Milan
PY  - 2015
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1276
AB  - Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17 degrees C for 3 days. Progressive motility was significantly higher (p<0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p<0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p<0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>= 65% progressive motility), was also significantly (p<0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%). These results show that seminal plasma proteins play an important role in maintaining the sperm progressive motility of diluted semen in vitro stored for 3 days.
PB  - Slovak Univ Agriculture Nitra, Nitra
T2  - Journal of Microbiology Biotechnology and Food Sciences
T1  - Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days
VL  - 4
SP  - 1
EP  - 3
DO  - 10.15414/jmbfs.2015.4.special2.1-3
ER  - 

@article{
author = "Apić, Jelena and Vakanjac, Slobodanka and Radović, Ivan and Jotanović, Stoja and Živkov-Baloš, Milica and Milovanović, Aleksandar and Barna, Tomislav and Stanković, Branislav and Jajić, Igor and Maletić, Milan",
year = "2015",
abstract = "Recently, it was frequently demonstrated that fertility of sows after artificially inseminated is lower than after mating. This is associated with a reduced fertilization capacity of overdiluted insemination doses. The aim of this study was to investigate the sperm motility in the semen samples, forming from the ejaculates with high or low protein content, stored in vitro on 17 degrees C for 3 days. Progressive motility was significantly higher (p<0.01) in the ejaculates with high, compared to the ejaculates with low protein content (82% vs. 76%). After 3 days of storage, in the1:4 dilution proportion, the average progressive motility was significantly (p<0.01) decreased in relation to this value in native semen from the boars with high (82% to 64%), as well from the boars with low protein content in seminal plasma (76% to 48%). However, the average diluted semen progressive motility was significantly greater (p<0.01) in the boars with high (64%), compared to the boars with low protein content in seminal plasma (48%). The number of good diluted semen samples (>= 65% progressive motility), was also significantly (p<0.01) greater in the boars with high (41%), compared to the boars with low protein content in seminal plasma (12%). These results show that seminal plasma proteins play an important role in maintaining the sperm progressive motility of diluted semen in vitro stored for 3 days.",
publisher = "Slovak Univ Agriculture Nitra, Nitra",
journal = "Journal of Microbiology Biotechnology and Food Sciences",
title = "Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days",
volume = "4",
pages = "1-3",
doi = "10.15414/jmbfs.2015.4.special2.1-3"
}

Apić, J., Vakanjac, S., Radović, I., Jotanović, S., Živkov-Baloš, M., Milovanović, A., Barna, T., Stanković, B., Jajić, I.,& Maletić, M.. (2015). Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days. in Journal of Microbiology Biotechnology and Food Sciences
Slovak Univ Agriculture Nitra, Nitra., 4, 1-3.
https://doi.org/10.15414/jmbfs.2015.4.special2.1-3

Apić J, Vakanjac S, Radović I, Jotanović S, Živkov-Baloš M, Milovanović A, Barna T, Stanković B, Jajić I, Maletić M. Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days. in Journal of Microbiology Biotechnology and Food Sciences. 2015;4:1-3.
doi:10.15414/jmbfs.2015.4.special2.1-3 .

Apić, Jelena, Vakanjac, Slobodanka, Radović, Ivan, Jotanović, Stoja, Živkov-Baloš, Milica, Milovanović, Aleksandar, Barna, Tomislav, Stanković, Branislav, Jajić, Igor, Maletić, Milan, "Effect of proteine content in boar seminal plasma on the sperm motility in diluted semen stored for 3 days" in Journal of Microbiology Biotechnology and Food Sciences, 4 (2015):1-3,
https://doi.org/10.15414/jmbfs.2015.4.special2.1-3 . .

TY  - JOUR
AU  - Milovanović, Aleksandar
AU  - Maksimović, N.
AU  - Barna, Tomislav
AU  - Lazarević, Miodrag
AU  - Delić, N.
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1025
AB  - This paper describes a procedure of laparoscopic insemination (LAI) performed in 42 Ile de France ewes with deep-frozen rams' semen. Frozen semen doses was imported from France, originating from two rams (A and B), and used intermittently in three LAI cycles on different females. The ewes were examined ultrasonographically for pregnancy, two months following insemination and data of lambing were recorded at the end. Total achieved lambing rate was 61.90% (26/42) and total number of lambs was 49 (prolificacy 1.88; 1.16 lambs per sheep included in LAI protocol). Three labs from 2 ewes were stillborn and one dyed at 10th day after lambing. Lambing rates for rams A and B were 53.85% (14/26) and 75.00% (12/16), respectively. Introduction of laparoscopic insemination of sheep with imported deep-frozen semen of top quality rams, gaining technical experience and achieving conception rate over 65% could make this technique acceptable in improving genetic potential of top quality sheep flocks.
AB  - U radu je opisan postupak laparoskopskog osemenjavanja (LAO) primenjen kod 42 ovce rase Ile de France duboko zamrznutim semenom. Tokom tri ciklusa osemenjavanja naizmenično je korišćeno seme dva ovna (A i B), uvezeno iz Francuske. Nakon dva meseca od osemenjavanja ovce su pregledane na sjagnjenost ultrasonografskom metodom i analizirani su podaci o jagnjenju. Ostvarena je koncepcija od 61,90% (26/42) i dobijeno je ukupno 49 jaganjaca (indeks jagnjena 1,88 po ojagnjenoj ovci, odnosno, 1,16 jagnjeta po ovci uključenoj u protokol LAO). Tri jagnjeta od dve ovce bila su mrtvorođena i jedno jagnje je uginulo 10. dana nakon jagnjenja. Sjagnjenost od semena ovna A iznosila je 58,85% (14/26) a od ovna B 75,00% (12/16). Uvođenje laparoskopskog osemenjavanja ovaca, uvoz duboko-zamrznutog semena vrhunskih ovnova, sticanje iskustva i ostvarenje koncepcije od preko 65% može ovu tehniku učiniti prihvatljivom u popravljanju genetskog potencijala kvalitetnih stada ovaca.
PB  - Institut za stočarstvo, Beograd
T2  - Biotechnology in Animal Husbandry
T1  - Laparoscopic insemination of sheep in Republic of Serbia
T1  - Laparoskopsko osemenjavanje ovaca u Republici Srbiji
VL  - 29
IS  - 3
SP  - 449
EP  - 456
DO  - 10.2298/BAH1303449M
ER  - 

@article{
author = "Milovanović, Aleksandar and Maksimović, N. and Barna, Tomislav and Lazarević, Miodrag and Delić, N.",
year = "2013",
abstract = "This paper describes a procedure of laparoscopic insemination (LAI) performed in 42 Ile de France ewes with deep-frozen rams' semen. Frozen semen doses was imported from France, originating from two rams (A and B), and used intermittently in three LAI cycles on different females. The ewes were examined ultrasonographically for pregnancy, two months following insemination and data of lambing were recorded at the end. Total achieved lambing rate was 61.90% (26/42) and total number of lambs was 49 (prolificacy 1.88; 1.16 lambs per sheep included in LAI protocol). Three labs from 2 ewes were stillborn and one dyed at 10th day after lambing. Lambing rates for rams A and B were 53.85% (14/26) and 75.00% (12/16), respectively. Introduction of laparoscopic insemination of sheep with imported deep-frozen semen of top quality rams, gaining technical experience and achieving conception rate over 65% could make this technique acceptable in improving genetic potential of top quality sheep flocks., U radu je opisan postupak laparoskopskog osemenjavanja (LAO) primenjen kod 42 ovce rase Ile de France duboko zamrznutim semenom. Tokom tri ciklusa osemenjavanja naizmenično je korišćeno seme dva ovna (A i B), uvezeno iz Francuske. Nakon dva meseca od osemenjavanja ovce su pregledane na sjagnjenost ultrasonografskom metodom i analizirani su podaci o jagnjenju. Ostvarena je koncepcija od 61,90% (26/42) i dobijeno je ukupno 49 jaganjaca (indeks jagnjena 1,88 po ojagnjenoj ovci, odnosno, 1,16 jagnjeta po ovci uključenoj u protokol LAO). Tri jagnjeta od dve ovce bila su mrtvorođena i jedno jagnje je uginulo 10. dana nakon jagnjenja. Sjagnjenost od semena ovna A iznosila je 58,85% (14/26) a od ovna B 75,00% (12/16). Uvođenje laparoskopskog osemenjavanja ovaca, uvoz duboko-zamrznutog semena vrhunskih ovnova, sticanje iskustva i ostvarenje koncepcije od preko 65% može ovu tehniku učiniti prihvatljivom u popravljanju genetskog potencijala kvalitetnih stada ovaca.",
publisher = "Institut za stočarstvo, Beograd",
journal = "Biotechnology in Animal Husbandry",
title = "Laparoscopic insemination of sheep in Republic of Serbia, Laparoskopsko osemenjavanje ovaca u Republici Srbiji",
volume = "29",
number = "3",
pages = "449-456",
doi = "10.2298/BAH1303449M"
}

Milovanović, A., Maksimović, N., Barna, T., Lazarević, M.,& Delić, N.. (2013). Laparoscopic insemination of sheep in Republic of Serbia. in Biotechnology in Animal Husbandry
Institut za stočarstvo, Beograd., 29(3), 449-456.
https://doi.org/10.2298/BAH1303449M

Milovanović A, Maksimović N, Barna T, Lazarević M, Delić N. Laparoscopic insemination of sheep in Republic of Serbia. in Biotechnology in Animal Husbandry. 2013;29(3):449-456.
doi:10.2298/BAH1303449M .

Milovanović, Aleksandar, Maksimović, N., Barna, Tomislav, Lazarević, Miodrag, Delić, N., "Laparoscopic insemination of sheep in Republic of Serbia" in Biotechnology in Animal Husbandry, 29, no. 3 (2013):449-456,
https://doi.org/10.2298/BAH1303449M . .

TY  - JOUR
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Milanov, Dubravka
AU  - Lazarević, Miodrag
PY  - 2013
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1072
AB  - In this article we presented procedures and results of boar semen quality control performed at the Scientific Veterinary Institute 'Novi Sad' based on continuous cooperation with the farms' centers for boar semen production. The data obtained by computer analysis (CASA-computer assisted sperm analysis), flow cytometry and cyto-morphologic examination were used for semen quality evaluation. The selected parameters were compared with the reproductive results in sows, such as: farrowing rate, number of piglets per litter, ratio of piglets born alive and stillborn piglets). Semen quality evaluation based on spermatozoa progressive motility, sperm concentration, morphological characteristics and chromatine structure damage were used to give recommendations for semen processing, dilution degree, prospective therapy of boars, or, at least, their culing. Analysis of semen was complemented with seasonal bacterial cultivation and controls in cases of sudden drop on semen quality. Separate files containing semen quality graphs and reproductive indicators for easier monitoring were created for every boar. Systematic semen analyses performed by the use of several modern methods, along with periodic bacteriological control, offer possibilities for reliable assessment of boars' semen quality.
AB  - U ovom radu su opisani postupak i rezultati kontrole kvaliteta semena nerastova u Naučnom institutu za veterinarstvo 'Novi Sad' na osnovu kontinuirane saradnje sa farmskim centrima za proizvodnju semena nerastova. U analizi kvaliteta semena je korišćena kompjuterska analiza (CASA-computer assysted sperm analysis), protočna citometrija i cito-morfološki pregled. Odabrani parametri kvaliteta semena su upoređivani sa reproduktivnim parametrima krmača kao što su oprasivost, broj prasadi po leglu, odnos živorođene i mrtvorođene prasadi. Ocena kvaliteta semena na osnovu progresivne pokretljivosti, koncentracije spermatozoida, morfoloških odlika i oštećenja hromatina je korišćena za davanje preporuka o načinu pripreme semena, određivanje stepena razređenja ili sprovođenje eventualne terapije nerastova, odnosno, njihovog isključenja iz priploda. Ove analize semena se dopunjavaju sezonskim bakteriološkim pregledom i kontrolom u slučaju pada kvaliteta semena. Za svakog nerasta je otvorena kartoteka kvaliteta semena sa grafičkim prikazom i reproduktivnim pokazateljima, radi lakšeg praćenja. Kontinuirana sistematska analiza kvaliteta semena, kombinovana sa više savremenih metoda, dopunjena povremenim bakteriološkim pregledom, daje mogućnost pouzdane procene kvaliteta semena nerastova.
PB  - Naučni institut za veterinarstvo "Novi Sad", Novi Sad
T2  - Arhiv veterinarske medicine
T1  - Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control
T1  - Model saradnje repro-centara i laboratorije za reprodukciju u kontroli kvaliteta semena nerastova
VL  - 6
IS  - 1
SP  - 57
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_1072
ER  - 

@article{
author = "Milovanović, Aleksandar and Barna, Tomislav and Milanov, Dubravka and Lazarević, Miodrag",
year = "2013",
abstract = "In this article we presented procedures and results of boar semen quality control performed at the Scientific Veterinary Institute 'Novi Sad' based on continuous cooperation with the farms' centers for boar semen production. The data obtained by computer analysis (CASA-computer assisted sperm analysis), flow cytometry and cyto-morphologic examination were used for semen quality evaluation. The selected parameters were compared with the reproductive results in sows, such as: farrowing rate, number of piglets per litter, ratio of piglets born alive and stillborn piglets). Semen quality evaluation based on spermatozoa progressive motility, sperm concentration, morphological characteristics and chromatine structure damage were used to give recommendations for semen processing, dilution degree, prospective therapy of boars, or, at least, their culing. Analysis of semen was complemented with seasonal bacterial cultivation and controls in cases of sudden drop on semen quality. Separate files containing semen quality graphs and reproductive indicators for easier monitoring were created for every boar. Systematic semen analyses performed by the use of several modern methods, along with periodic bacteriological control, offer possibilities for reliable assessment of boars' semen quality., U ovom radu su opisani postupak i rezultati kontrole kvaliteta semena nerastova u Naučnom institutu za veterinarstvo 'Novi Sad' na osnovu kontinuirane saradnje sa farmskim centrima za proizvodnju semena nerastova. U analizi kvaliteta semena je korišćena kompjuterska analiza (CASA-computer assysted sperm analysis), protočna citometrija i cito-morfološki pregled. Odabrani parametri kvaliteta semena su upoređivani sa reproduktivnim parametrima krmača kao što su oprasivost, broj prasadi po leglu, odnos živorođene i mrtvorođene prasadi. Ocena kvaliteta semena na osnovu progresivne pokretljivosti, koncentracije spermatozoida, morfoloških odlika i oštećenja hromatina je korišćena za davanje preporuka o načinu pripreme semena, određivanje stepena razređenja ili sprovođenje eventualne terapije nerastova, odnosno, njihovog isključenja iz priploda. Ove analize semena se dopunjavaju sezonskim bakteriološkim pregledom i kontrolom u slučaju pada kvaliteta semena. Za svakog nerasta je otvorena kartoteka kvaliteta semena sa grafičkim prikazom i reproduktivnim pokazateljima, radi lakšeg praćenja. Kontinuirana sistematska analiza kvaliteta semena, kombinovana sa više savremenih metoda, dopunjena povremenim bakteriološkim pregledom, daje mogućnost pouzdane procene kvaliteta semena nerastova.",
publisher = "Naučni institut za veterinarstvo "Novi Sad", Novi Sad",
journal = "Arhiv veterinarske medicine",
title = "Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control, Model saradnje repro-centara i laboratorije za reprodukciju u kontroli kvaliteta semena nerastova",
volume = "6",
number = "1",
pages = "57-70",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_1072"
}

Milovanović, A., Barna, T., Milanov, D.,& Lazarević, M.. (2013). Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control. in Arhiv veterinarske medicine
Naučni institut za veterinarstvo "Novi Sad", Novi Sad., 6(1), 57-70.
https://hdl.handle.net/21.15107/rcub_veterinar_1072

Milovanović A, Barna T, Milanov D, Lazarević M. Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control. in Arhiv veterinarske medicine. 2013;6(1):57-70.
https://hdl.handle.net/21.15107/rcub_veterinar_1072 .

Milovanović, Aleksandar, Barna, Tomislav, Milanov, Dubravka, Lazarević, Miodrag, "Model for cooperation between boar studs and laboratories for reproduction in boars' semen quality control" in Arhiv veterinarske medicine, 6, no. 1 (2013):57-70,
https://hdl.handle.net/21.15107/rcub_veterinar_1072 .

TY  - JOUR
AU  - Lazarević, Miodrag
AU  - Milovanović, Aleksandar
AU  - Barna, Tomislav
AU  - Miljas, N.
AU  - Milanov, Dubravka
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/917
AB  - On the basis of our investigations it was possible to conclude that intrauterine treatment of sows with puerperal uterine infections with sterile YCW (Yeast Cell Wall) resulted in significant clinical improvement. The percent of recidivism was the lowest (10%) in groups of sows treated with 10 and 20 g of YCW. The degree of bacterial CFU (Colony Forming Units) reduction in samples of sows uterine flushings following instillation of YCW (5, 10 and 20 g) was wery high and ranged from 1361 to 1444 times, while in sows treated with Lotagen 2% solution (100 mL) this parametar was only 32. At the moment of weaning, piglets from sows treated with 10 and 20 g of YCW were heavier when compared to the control and Lotagen group and their DBWG (Daily Body Weight Gain) was higher when compared to the Lotagen and control group. Treatment of sows by IU instillation of YCW did not influence the number of piglets in the next breeding cycle.
AB  - Na osnovu rezultata naših istraživanja bilo je moguće zaključiti da tretman krmača obolelih od puerperalnih infekcija materice, sterilnim preparatom dobijenim iz zida kvasca (YCW), ima za posledicu značajan klinički napredak. Procenat krmača sa recidivom je bio najniži (10%) u grupi tretiranoj sa 10 i 20g YCW. Stepen redukcije broja bakterijskih kolonija (CFU) u uzorcima dobijenih iz materice krmača posle tretmana YCW preparatom je bio veoma visok i kretao se u opsegu od 1361 do 1444 puta. Kod obolelih krmača, tretiranih rastvorom Lotagena (2%), vrednost ovog parametra je bila svega 32. U momentu zalučenja, prasad krmača tretiranih YCW preparatom u količini od 10 i 20 g bila su teža u poređenju sa prasadima krmača grupe tretirane Lotagenom i prasadima kontrolnih (neteretiranih) krmača. Osim toga i njihovi prosečni dnevni prirasti su bili veći. Tretman krmača intrauterinom instilacijom preparata na bazi YCW nije negativno uticao na broj prasadi u sledećem reproduktivnom ciklusu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution
T1  - Terapija endometritisa krmača intrauterinom instilacijom preparata na bazi zida kvasca
VL  - 62
IS  - 5-6
SP  - 611
EP  - 626
DO  - 10.2298/AVB1206611L
ER  - 

@article{
author = "Lazarević, Miodrag and Milovanović, Aleksandar and Barna, Tomislav and Miljas, N. and Milanov, Dubravka",
year = "2012",
abstract = "On the basis of our investigations it was possible to conclude that intrauterine treatment of sows with puerperal uterine infections with sterile YCW (Yeast Cell Wall) resulted in significant clinical improvement. The percent of recidivism was the lowest (10%) in groups of sows treated with 10 and 20 g of YCW. The degree of bacterial CFU (Colony Forming Units) reduction in samples of sows uterine flushings following instillation of YCW (5, 10 and 20 g) was wery high and ranged from 1361 to 1444 times, while in sows treated with Lotagen 2% solution (100 mL) this parametar was only 32. At the moment of weaning, piglets from sows treated with 10 and 20 g of YCW were heavier when compared to the control and Lotagen group and their DBWG (Daily Body Weight Gain) was higher when compared to the Lotagen and control group. Treatment of sows by IU instillation of YCW did not influence the number of piglets in the next breeding cycle., Na osnovu rezultata naših istraživanja bilo je moguće zaključiti da tretman krmača obolelih od puerperalnih infekcija materice, sterilnim preparatom dobijenim iz zida kvasca (YCW), ima za posledicu značajan klinički napredak. Procenat krmača sa recidivom je bio najniži (10%) u grupi tretiranoj sa 10 i 20g YCW. Stepen redukcije broja bakterijskih kolonija (CFU) u uzorcima dobijenih iz materice krmača posle tretmana YCW preparatom je bio veoma visok i kretao se u opsegu od 1361 do 1444 puta. Kod obolelih krmača, tretiranih rastvorom Lotagena (2%), vrednost ovog parametra je bila svega 32. U momentu zalučenja, prasad krmača tretiranih YCW preparatom u količini od 10 i 20 g bila su teža u poređenju sa prasadima krmača grupe tretirane Lotagenom i prasadima kontrolnih (neteretiranih) krmača. Osim toga i njihovi prosečni dnevni prirasti su bili veći. Tretman krmača intrauterinom instilacijom preparata na bazi YCW nije negativno uticao na broj prasadi u sledećem reproduktivnom ciklusu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution, Terapija endometritisa krmača intrauterinom instilacijom preparata na bazi zida kvasca",
volume = "62",
number = "5-6",
pages = "611-626",
doi = "10.2298/AVB1206611L"
}

Lazarević, M., Milovanović, A., Barna, T., Miljas, N.,& Milanov, D.. (2012). Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 611-626.
https://doi.org/10.2298/AVB1206611L

Lazarević M, Milovanović A, Barna T, Miljas N, Milanov D. Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution. in Acta Veterinaria-Beograd. 2012;62(5-6):611-626.
doi:10.2298/AVB1206611L .

Lazarević, Miodrag, Milovanović, Aleksandar, Barna, Tomislav, Miljas, N., Milanov, Dubravka, "Endometritis therapy in sows by intra uterine instillation of yeast cell wall solution" in Acta Veterinaria-Beograd, 62, no. 5-6 (2012):611-626,
https://doi.org/10.2298/AVB1206611L . .

TY  - JOUR
AU  - Gvozdić, Dragan
AU  - Stančić, Ivan
AU  - Savović, Milan
AU  - Stančić, Blagoje
AU  - Božić, Aleksandar
AU  - Milanović, Svetlana
AU  - Jovanović, Ivan B.
AU  - Barna, Tomislav
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/845
AB  - The aim of this work was to determine incidence of calving and postcalving reproduction disorders and their effect on the major parameters of cows reproductive efficiency at dairy farm in the R. Serbia. Total number of 1835 high-milking dairy cows (av. milk production >8500L/305d of lactation) records has been reviewed during one year period, at the large dairy farm in area of Vojvodina (R. Serbia). Normal calving and puerperal period without health problems was observed at 583 cows (27,6%), while different abnormalities during and after calving were recorded in 1252 cows (72,4%). The highest percentages of registered peripartal disorders were dystocia and endometritis (62% and 64%, respectively), affecting almost the same percentage of cows. Peripartal disorders had statistically significant influence (P lt 0.05) on the increase of number of services/conception (3,5 vs. 2,7, reproductive disorders vs. healthy cows, respectively), prolongation of the interval from first postpartal insemination till conception (110 vs. 57 days, reproductive disorders vs. healthy cows, respectively), and increase of days open period (214 vs. 146 days, reproductive disorders vs. healthy cows, respectively). This results have confirmed increased incidence of reproduction disorders during calving and postcalving period that significantly reduces reproductive efficiency in high milking dairy cows after parturition.
AB  - Istraživanja u mnogim zemljama, tokom poslednjih decenija, pokazuju permanentan pad reproduktivne efikasnosti krava visoke mlečnosti. Cilj ovog rada je da se utvrdi da li i u kojoj meri, peripartalni poremećaji imaju uticaja na osnovne parametre reproduktivne efikasnosti krava u našim proizvodnim uslovima. Istraživanjem je obuhvaćeno ukupno 1835 krava, tokom jedne godine, na jednoj velikoj farmi sa visokom proizvodnjom mleka (prosečna proizvodnja >8500L/305 dana laktacije) u regionu Vojvodine (Republika Srbija). Od ukupnog broja ispitivanih krava, telenje je prošlo bez poremećaja kod 583 krave (27,6%), a različiti poremećaji tokom i posle telenja su ustanovljeni kod 1.252 krave (72,4%). Najčešće ustanovljeni peripartalni poremećaji kod visoko mlečnih krava su teško teljenje (62%) i endometritis (64%). Peripartalni poremećaji imaju statistički značajan uticaj (P lt 0,05) na povećanje indeksa osemenjavanja (3,5 prema 2,7), produženje intervala od prvog do fertilnog osemenjavanja (57 prema 110 dana), kao i na produženje trajanja servis perioda (146 prema 214 dana), u poređenju sa kravama bez poremećaja. Dobijeni rezultati pokazuju da na farmama visoko mlečnih krava povišena učestalost peripartalnih reproduktivnih poremećaja koji značajno utiču na smanjenje reproduktivne efikasnosti krava nakon partusa.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Savremena poljoprivreda
T1  - Reproductive efficiency in high-milking dairy cows after calving
T1  - Reproduktivna efikasnost visoko-mlečnih krava posle telenja
VL  - 60
IS  - 1-2
SP  - 86
EP  - 97
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_845
ER  - 

@article{
author = "Gvozdić, Dragan and Stančić, Ivan and Savović, Milan and Stančić, Blagoje and Božić, Aleksandar and Milanović, Svetlana and Jovanović, Ivan B. and Barna, Tomislav",
year = "2011",
abstract = "The aim of this work was to determine incidence of calving and postcalving reproduction disorders and their effect on the major parameters of cows reproductive efficiency at dairy farm in the R. Serbia. Total number of 1835 high-milking dairy cows (av. milk production >8500L/305d of lactation) records has been reviewed during one year period, at the large dairy farm in area of Vojvodina (R. Serbia). Normal calving and puerperal period without health problems was observed at 583 cows (27,6%), while different abnormalities during and after calving were recorded in 1252 cows (72,4%). The highest percentages of registered peripartal disorders were dystocia and endometritis (62% and 64%, respectively), affecting almost the same percentage of cows. Peripartal disorders had statistically significant influence (P lt 0.05) on the increase of number of services/conception (3,5 vs. 2,7, reproductive disorders vs. healthy cows, respectively), prolongation of the interval from first postpartal insemination till conception (110 vs. 57 days, reproductive disorders vs. healthy cows, respectively), and increase of days open period (214 vs. 146 days, reproductive disorders vs. healthy cows, respectively). This results have confirmed increased incidence of reproduction disorders during calving and postcalving period that significantly reduces reproductive efficiency in high milking dairy cows after parturition., Istraživanja u mnogim zemljama, tokom poslednjih decenija, pokazuju permanentan pad reproduktivne efikasnosti krava visoke mlečnosti. Cilj ovog rada je da se utvrdi da li i u kojoj meri, peripartalni poremećaji imaju uticaja na osnovne parametre reproduktivne efikasnosti krava u našim proizvodnim uslovima. Istraživanjem je obuhvaćeno ukupno 1835 krava, tokom jedne godine, na jednoj velikoj farmi sa visokom proizvodnjom mleka (prosečna proizvodnja >8500L/305 dana laktacije) u regionu Vojvodine (Republika Srbija). Od ukupnog broja ispitivanih krava, telenje je prošlo bez poremećaja kod 583 krave (27,6%), a različiti poremećaji tokom i posle telenja su ustanovljeni kod 1.252 krave (72,4%). Najčešće ustanovljeni peripartalni poremećaji kod visoko mlečnih krava su teško teljenje (62%) i endometritis (64%). Peripartalni poremećaji imaju statistički značajan uticaj (P lt 0,05) na povećanje indeksa osemenjavanja (3,5 prema 2,7), produženje intervala od prvog do fertilnog osemenjavanja (57 prema 110 dana), kao i na produženje trajanja servis perioda (146 prema 214 dana), u poređenju sa kravama bez poremećaja. Dobijeni rezultati pokazuju da na farmama visoko mlečnih krava povišena učestalost peripartalnih reproduktivnih poremećaja koji značajno utiču na smanjenje reproduktivne efikasnosti krava nakon partusa.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Savremena poljoprivreda",
title = "Reproductive efficiency in high-milking dairy cows after calving, Reproduktivna efikasnost visoko-mlečnih krava posle telenja",
volume = "60",
number = "1-2",
pages = "86-97",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_845"
}

Gvozdić, D., Stančić, I., Savović, M., Stančić, B., Božić, A., Milanović, S., Jovanović, I. B.,& Barna, T.. (2011). Reproductive efficiency in high-milking dairy cows after calving. in Savremena poljoprivreda
Univerzitet u Novom Sadu - Poljoprivredni fakultet, Novi Sad i Naučni institut za ratarstvo i povrtarstvo, Novi Sad., 60(1-2), 86-97.
https://hdl.handle.net/21.15107/rcub_veterinar_845

Gvozdić D, Stančić I, Savović M, Stančić B, Božić A, Milanović S, Jovanović IB, Barna T. Reproductive efficiency in high-milking dairy cows after calving. in Savremena poljoprivreda. 2011;60(1-2):86-97.
https://hdl.handle.net/21.15107/rcub_veterinar_845 .

Gvozdić, Dragan, Stančić, Ivan, Savović, Milan, Stančić, Blagoje, Božić, Aleksandar, Milanović, Svetlana, Jovanović, Ivan B., Barna, Tomislav, "Reproductive efficiency in high-milking dairy cows after calving" in Savremena poljoprivreda, 60, no. 1-2 (2011):86-97,
https://hdl.handle.net/21.15107/rcub_veterinar_845 .

TY  - CONF
AU  - Milovanović, A.
AU  - Barna, Tomislav
AU  - Jovičin, Milovan
AU  - Lazarević, Miodrag
AU  - Nakao, T.
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/714
PB  - Wiley-Blackwell Publishing, Inc, Malden
C3  - Reproduction in Domestic Animals
T1  - Influence of beta-carotene and human chorionic gonadotropin on milk progesterone level and pregnancy rate in Holstein cows
VL  - 45
SP  - 99
EP  - 99
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_714
ER  - 

@conference{
author = "Milovanović, A. and Barna, Tomislav and Jovičin, Milovan and Lazarević, Miodrag and Nakao, T.",
year = "2010",
publisher = "Wiley-Blackwell Publishing, Inc, Malden",
journal = "Reproduction in Domestic Animals",
title = "Influence of beta-carotene and human chorionic gonadotropin on milk progesterone level and pregnancy rate in Holstein cows",
volume = "45",
pages = "99-99",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_714"
}

Milovanović, A., Barna, T., Jovičin, M., Lazarević, M.,& Nakao, T.. (2010). Influence of beta-carotene and human chorionic gonadotropin on milk progesterone level and pregnancy rate in Holstein cows. in Reproduction in Domestic Animals
Wiley-Blackwell Publishing, Inc, Malden., 45, 99-99.
https://hdl.handle.net/21.15107/rcub_veterinar_714

Milovanović A, Barna T, Jovičin M, Lazarević M, Nakao T. Influence of beta-carotene and human chorionic gonadotropin on milk progesterone level and pregnancy rate in Holstein cows. in Reproduction in Domestic Animals. 2010;45:99-99.
https://hdl.handle.net/21.15107/rcub_veterinar_714 .

Milovanović, A., Barna, Tomislav, Jovičin, Milovan, Lazarević, Miodrag, Nakao, T., "Influence of beta-carotene and human chorionic gonadotropin on milk progesterone level and pregnancy rate in Holstein cows" in Reproduction in Domestic Animals, 45 (2010):99-99,
https://hdl.handle.net/21.15107/rcub_veterinar_714 .

TY  - JOUR
AU  - Milovanović, Aleksandar
AU  - Jovičin, Milovan
AU  - Barna, Tomislav
AU  - Golubović, Snežana
AU  - Resanović, Vojislav
AU  - Rodić, Vladimir
AU  - Kitanović, Smiljka
AU  - Lazarević, Miodrag
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/626
AB  - In this work the possibility of producing and using our new EIA (enzyme immunosorbent test) set for direct determination of progesterone concentration in whole milk was analyzed. The test confirmed high values in milk of confirmed gravid cows (13,16± 13,29 ng/ml; n=10) and confirmed non-gravid cows (1,35±0,98 ng/ml; n=8). This progesterone test helps following variations of progesterone concentration in blood and milk cow samples taken every second day. The progesterone concentration of gravid and low values of non-gravid cows are displayed, as well as errors in cycles and mistakes in insemination. The results were compared to the results obtained by the RIA progesterone test done on blood sera samples. Progesterone concentration measured with EIA test in 15 milk samples was compared to the values of progesterone obtained by a commercial EIA test. The differences were not statistically significant (t-test, t-0.1144; p-0.9097). After milking, the samples were stored for 28 days at 4oC, conserved by potassium dichromate, and a loss of progesterone activity by 25,90±15,47% (4,69±2,90 ng/ml) was noticed, but in the final outcome this did not influence the clinical results. Accordingly, milk samples with preservative may be stored in a refrigerator for four weeks.
AB  - U ovom radu prikazani su rezultati primene našeg novog enzimskog imunosorbent testa (EIA) za direktno određivanje koncentracije progesterona u punom mleku krava. Testom su potvrđene visoke koncentracije u mleku kod steonih krava (13,16±13,29 ng/ml; n=10) i niske koncentracije kod nesteonih krava (1,35±0,98 ng/ml; n=8). Ovim progesteronskim testom se u uzorcima mleka mogu pouzdano pratiti variranja u koncentraciji progesterona po danima ciklusa krava, ako se uzorci uzimaju svakog drugog dana. U radu su prikazani i progesteronski profili steonih i nesteonih krava, kao i profili onih sa poremećenim ciklusom i sa greškom u osemenjavanju. Rezultati dobijeni EIA testom su upoređivani sa rezultatima dobijenim komercijalnim RIA-progesteronskim setom iz uzoraka krvnog seruma. Razlike u koncentraciji progesterona u 15 uzoraka mleka krava novoproizvedenim EIA testom i koncentraciji određivanoj komercijalnim EIA testom nisu bile statistički značajne (t-test: t-0,1144; p-0,9097). U uzorcima mleka čuvanim na temperaturi frižidera (+4oC) konzervisanim kalijum bihromatom (do 28. dana nakon muže) ustanovljene su niže koncentracije progesterona za 25,90±15,47% (4,69± 2,90 ng/ml) u odnosu na koncentraciju na dan muže, što u krajnjem ishodu ipak nije uticalo na dobijene kliničke rezultate. Za primenu u EIA testu uzorci mleka sa konzervansom mogu se skladištiti četiri nedelje u frižideru.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Applying enzyme immunotest for determination of progesterone concentration in whole cow 's milk
T1  - Primena enzimskog imunotesta za određivanje koncentracije progesterona u punom kravljem mleku
VL  - 63
IS  - 1-2
SP  - 45
EP  - 59
DO  - 10.2298/VETGL0902045M
ER  - 

@article{
author = "Milovanović, Aleksandar and Jovičin, Milovan and Barna, Tomislav and Golubović, Snežana and Resanović, Vojislav and Rodić, Vladimir and Kitanović, Smiljka and Lazarević, Miodrag",
year = "2009",
abstract = "In this work the possibility of producing and using our new EIA (enzyme immunosorbent test) set for direct determination of progesterone concentration in whole milk was analyzed. The test confirmed high values in milk of confirmed gravid cows (13,16± 13,29 ng/ml; n=10) and confirmed non-gravid cows (1,35±0,98 ng/ml; n=8). This progesterone test helps following variations of progesterone concentration in blood and milk cow samples taken every second day. The progesterone concentration of gravid and low values of non-gravid cows are displayed, as well as errors in cycles and mistakes in insemination. The results were compared to the results obtained by the RIA progesterone test done on blood sera samples. Progesterone concentration measured with EIA test in 15 milk samples was compared to the values of progesterone obtained by a commercial EIA test. The differences were not statistically significant (t-test, t-0.1144; p-0.9097). After milking, the samples were stored for 28 days at 4oC, conserved by potassium dichromate, and a loss of progesterone activity by 25,90±15,47% (4,69±2,90 ng/ml) was noticed, but in the final outcome this did not influence the clinical results. Accordingly, milk samples with preservative may be stored in a refrigerator for four weeks., U ovom radu prikazani su rezultati primene našeg novog enzimskog imunosorbent testa (EIA) za direktno određivanje koncentracije progesterona u punom mleku krava. Testom su potvrđene visoke koncentracije u mleku kod steonih krava (13,16±13,29 ng/ml; n=10) i niske koncentracije kod nesteonih krava (1,35±0,98 ng/ml; n=8). Ovim progesteronskim testom se u uzorcima mleka mogu pouzdano pratiti variranja u koncentraciji progesterona po danima ciklusa krava, ako se uzorci uzimaju svakog drugog dana. U radu su prikazani i progesteronski profili steonih i nesteonih krava, kao i profili onih sa poremećenim ciklusom i sa greškom u osemenjavanju. Rezultati dobijeni EIA testom su upoređivani sa rezultatima dobijenim komercijalnim RIA-progesteronskim setom iz uzoraka krvnog seruma. Razlike u koncentraciji progesterona u 15 uzoraka mleka krava novoproizvedenim EIA testom i koncentraciji određivanoj komercijalnim EIA testom nisu bile statistički značajne (t-test: t-0,1144; p-0,9097). U uzorcima mleka čuvanim na temperaturi frižidera (+4oC) konzervisanim kalijum bihromatom (do 28. dana nakon muže) ustanovljene su niže koncentracije progesterona za 25,90±15,47% (4,69± 2,90 ng/ml) u odnosu na koncentraciju na dan muže, što u krajnjem ishodu ipak nije uticalo na dobijene kliničke rezultate. Za primenu u EIA testu uzorci mleka sa konzervansom mogu se skladištiti četiri nedelje u frižideru.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Applying enzyme immunotest for determination of progesterone concentration in whole cow 's milk, Primena enzimskog imunotesta za određivanje koncentracije progesterona u punom kravljem mleku",
volume = "63",
number = "1-2",
pages = "45-59",
doi = "10.2298/VETGL0902045M"
}

Milovanović, A., Jovičin, M., Barna, T., Golubović, S., Resanović, V., Rodić, V., Kitanović, S.,& Lazarević, M.. (2009). Applying enzyme immunotest for determination of progesterone concentration in whole cow 's milk. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(1-2), 45-59.
https://doi.org/10.2298/VETGL0902045M

Milovanović A, Jovičin M, Barna T, Golubović S, Resanović V, Rodić V, Kitanović S, Lazarević M. Applying enzyme immunotest for determination of progesterone concentration in whole cow 's milk. in Veterinarski Glasnik. 2009;63(1-2):45-59.
doi:10.2298/VETGL0902045M .

Milovanović, Aleksandar, Jovičin, Milovan, Barna, Tomislav, Golubović, Snežana, Resanović, Vojislav, Rodić, Vladimir, Kitanović, Smiljka, Lazarević, Miodrag, "Applying enzyme immunotest for determination of progesterone concentration in whole cow 's milk" in Veterinarski Glasnik, 63, no. 1-2 (2009):45-59,
https://doi.org/10.2298/VETGL0902045M . .