TY  - JOUR
AU  - Radovanović, Anita
AU  - Roksandić, Dragutin
AU  - Šimić, Marija
AU  - Marković, Danica
AU  - Gledić, Dušan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/941
AB  - The effects of propylthyouracil (PTU) induced hypothyroidism of rats during pregnancy and lactation on offspring ovarian development and maturation were studied. Thyroid hormones and thyroid stimulating hormone (TSH) concentrations were determined using the radioimmunoassay method in order to verify the hypothyroid status of treated mothers and their two months old pups. The ovaries of the offspring were processed for light microscopy analysis on the day of the first estrus after the 60th day of age. Histological analysis including follicle count was performed on serial sections stained with haematoxyline/eosin and on semithin sections stained with methylene blue. A significant increase of serum TSH and decrease in T3 and T4 levels was observed in treated mothers compared to controls. The levels of measured hormones in the control and PTU-treated two months old rats were not significantly different. Ten percent of 60-dayold treated females did not reach estrus and they were sacrificed in diestrus. The secondary interstitial cells were the dominant structures in the ovaries. The number of healthy growing and early antral follicles was markedly decreased. Ovaries of treated rats contained relatively few antral follicles, significantly more atretic antral follicles and a decreased number of corpora lutea, compared to controls. These results indicate that lack of thyroid hormones during prenatal and early postnatal development impair ovarian development in rats.
AB  - U okviru ovog rada izučavan je uticaj hipotireoidizma majki izazvanog propiltiouracilom (PTU) tokom graviditeta i laktacije na razvoj i sazrevanje jajnika pacova. U cilju određivanja tireoidnog statusa majki i mladunaca, merena je koncentracija tireoidnih hormona i tireostimulirajućeg hormona (TSH) u serumu. Jajnici potomaka hipotireoidnih majki i kontrolne grupe sprovedeni su za analizu svetlosnom mikroskopijom na dan prvog estrusa posle 60. dana starosti. Analizirani su serijski isečci jajnika bojeni hematoksilinom i eozinom, kao i polutanki preseci bojeni metilenskim plavim. Koncentracija T4 i T3 značajno je smanjena u serumu tretiranih majki, dok je nivo TSH značajno povišen u odnosu na kontrole. Kod dva meseca starih kontrolnih i tretiranih ženskih potomaka nije bilo značajne razlike u nivou svih određivanih hormona. Deset procenata tretiranih životinja nije normalno ušlo u estrus do 60. dana, i žrtvovane su u fazi diestrusa. Sekundarne intersticijalne ćelije su dominantne strukture u jajnicima tretiranih pacova. Ukupan broj aktiviranih folikula je značajno smanjen uz izrazitu atreziju folikula, a smanjen je i broj žutih tela. Rezultati pokazuju da hipotireoidizam izazvan u toku graviditeta i laktacije dovodi do promena u razviću jajnika.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effects of induced maternal hypothyroidism on the ovarian development of offspring rats
T1  - Uticaj indukovanog hipotireoidizma majki na razvoj jajnika mladunaca pacova
VL  - 62
IS  - 5-6
SP  - 483
EP  - 493
DO  - 10.2298/AVB1206483R
ER  - 

@article{
author = "Radovanović, Anita and Roksandić, Dragutin and Šimić, Marija and Marković, Danica and Gledić, Dušan",
year = "2012",
abstract = "The effects of propylthyouracil (PTU) induced hypothyroidism of rats during pregnancy and lactation on offspring ovarian development and maturation were studied. Thyroid hormones and thyroid stimulating hormone (TSH) concentrations were determined using the radioimmunoassay method in order to verify the hypothyroid status of treated mothers and their two months old pups. The ovaries of the offspring were processed for light microscopy analysis on the day of the first estrus after the 60th day of age. Histological analysis including follicle count was performed on serial sections stained with haematoxyline/eosin and on semithin sections stained with methylene blue. A significant increase of serum TSH and decrease in T3 and T4 levels was observed in treated mothers compared to controls. The levels of measured hormones in the control and PTU-treated two months old rats were not significantly different. Ten percent of 60-dayold treated females did not reach estrus and they were sacrificed in diestrus. The secondary interstitial cells were the dominant structures in the ovaries. The number of healthy growing and early antral follicles was markedly decreased. Ovaries of treated rats contained relatively few antral follicles, significantly more atretic antral follicles and a decreased number of corpora lutea, compared to controls. These results indicate that lack of thyroid hormones during prenatal and early postnatal development impair ovarian development in rats., U okviru ovog rada izučavan je uticaj hipotireoidizma majki izazvanog propiltiouracilom (PTU) tokom graviditeta i laktacije na razvoj i sazrevanje jajnika pacova. U cilju određivanja tireoidnog statusa majki i mladunaca, merena je koncentracija tireoidnih hormona i tireostimulirajućeg hormona (TSH) u serumu. Jajnici potomaka hipotireoidnih majki i kontrolne grupe sprovedeni su za analizu svetlosnom mikroskopijom na dan prvog estrusa posle 60. dana starosti. Analizirani su serijski isečci jajnika bojeni hematoksilinom i eozinom, kao i polutanki preseci bojeni metilenskim plavim. Koncentracija T4 i T3 značajno je smanjena u serumu tretiranih majki, dok je nivo TSH značajno povišen u odnosu na kontrole. Kod dva meseca starih kontrolnih i tretiranih ženskih potomaka nije bilo značajne razlike u nivou svih određivanih hormona. Deset procenata tretiranih životinja nije normalno ušlo u estrus do 60. dana, i žrtvovane su u fazi diestrusa. Sekundarne intersticijalne ćelije su dominantne strukture u jajnicima tretiranih pacova. Ukupan broj aktiviranih folikula je značajno smanjen uz izrazitu atreziju folikula, a smanjen je i broj žutih tela. Rezultati pokazuju da hipotireoidizam izazvan u toku graviditeta i laktacije dovodi do promena u razviću jajnika.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effects of induced maternal hypothyroidism on the ovarian development of offspring rats, Uticaj indukovanog hipotireoidizma majki na razvoj jajnika mladunaca pacova",
volume = "62",
number = "5-6",
pages = "483-493",
doi = "10.2298/AVB1206483R"
}

Radovanović, A., Roksandić, D., Šimić, M., Marković, D.,& Gledić, D.. (2012). Effects of induced maternal hypothyroidism on the ovarian development of offspring rats. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 483-493.
https://doi.org/10.2298/AVB1206483R

Radovanović A, Roksandić D, Šimić M, Marković D, Gledić D. Effects of induced maternal hypothyroidism on the ovarian development of offspring rats. in Acta Veterinaria-Beograd. 2012;62(5-6):483-493.
doi:10.2298/AVB1206483R .

Radovanović, Anita, Roksandić, Dragutin, Šimić, Marija, Marković, Danica, Gledić, Dušan, "Effects of induced maternal hypothyroidism on the ovarian development of offspring rats" in Acta Veterinaria-Beograd, 62, no. 5-6 (2012):483-493,
https://doi.org/10.2298/AVB1206483R . .

TY  - JOUR
AU  - Roksandić, Dragutin
AU  - Šimić, Marija
AU  - Radovanović, Anita
AU  - Gledić, Dušan
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/395
AB  - In this paper, the effect of hypothyroidism on body mass and spleen mass of rats was examined during the prenatal and early juvenile periods. Hypothyroidism was induced by the application of propylthiouracil (PTU) in drinking water to the mothers from the first day of gravidity and during lactation, and the offspring were sacrificed on the 14th and 21st days after birth. The body mass of the juvenile rats was measured just before they were sacrificed. The concentrations of triiodothyronine (T3) and thyroxine (T4) in blood serum were determined in control and treated juvenile rats. The results indicate that PTU leads to a reduction in T3 and T4 serum concentrations in treated juvenile rats. Treated juvenile rats had a bigger body mass and spleen mass in comparison with control animals. These data indicate that hypothyroidism induced in the prenatal and early juvenile period leads to an increase in the body mass and spleen mass and disrupts the normal development of the spleen in the course of the examined period. .
AB  - U ovom radu ispitivan je uticaj hipotireoidizma na telesnu masu i masu slezine pacova u prenatalnom i ranom juvenilnom periodu. Hipotireoidizam je indukovan aplikacijom propiltiouracila (PTU) u vodu za piće majkama od prvog dana graviditeta i tokom laktacije, a mladunci su žrtvovani 14. i 21. dana nakon rođenja. Tretirani mladunci su imali veću telesnu masu i masu slezine u odnosu na kontrolne mladunče. U krvnom serumu određena je koncentracija trijodtironina (T3) i tiroksina (TJ kontrolnim i tretiranim mladuncima. Rezultati ukazuju da PTU uzrokuje smanjenje koncentracije T3 i 74 u serumu tretiranih mladunaca. Ovi podaci ukazuju da hipotireoidizam indukovan u prenatalnom i ranom juvenilnom periodu dovodi do povećanja telesne mase i mase slezine i remeti pravilno razviće slezine tokom perioda ispitivanja. .
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Body mass, spleen mass and level of thyroid hormones in juvenile hypothyroid rats
T1  - Telesna masa, masa slezine i nivo tireoidnih hormona kod juvenilnih hipotireoidnih pacova
VL  - 60
IS  - 3-4
SP  - 153
EP  - 162
DO  - 10.2298/VETGL0604153R
ER  - 

@article{
author = "Roksandić, Dragutin and Šimić, Marija and Radovanović, Anita and Gledić, Dušan",
year = "2006",
abstract = "In this paper, the effect of hypothyroidism on body mass and spleen mass of rats was examined during the prenatal and early juvenile periods. Hypothyroidism was induced by the application of propylthiouracil (PTU) in drinking water to the mothers from the first day of gravidity and during lactation, and the offspring were sacrificed on the 14th and 21st days after birth. The body mass of the juvenile rats was measured just before they were sacrificed. The concentrations of triiodothyronine (T3) and thyroxine (T4) in blood serum were determined in control and treated juvenile rats. The results indicate that PTU leads to a reduction in T3 and T4 serum concentrations in treated juvenile rats. Treated juvenile rats had a bigger body mass and spleen mass in comparison with control animals. These data indicate that hypothyroidism induced in the prenatal and early juvenile period leads to an increase in the body mass and spleen mass and disrupts the normal development of the spleen in the course of the examined period. ., U ovom radu ispitivan je uticaj hipotireoidizma na telesnu masu i masu slezine pacova u prenatalnom i ranom juvenilnom periodu. Hipotireoidizam je indukovan aplikacijom propiltiouracila (PTU) u vodu za piće majkama od prvog dana graviditeta i tokom laktacije, a mladunci su žrtvovani 14. i 21. dana nakon rođenja. Tretirani mladunci su imali veću telesnu masu i masu slezine u odnosu na kontrolne mladunče. U krvnom serumu određena je koncentracija trijodtironina (T3) i tiroksina (TJ kontrolnim i tretiranim mladuncima. Rezultati ukazuju da PTU uzrokuje smanjenje koncentracije T3 i 74 u serumu tretiranih mladunaca. Ovi podaci ukazuju da hipotireoidizam indukovan u prenatalnom i ranom juvenilnom periodu dovodi do povećanja telesne mase i mase slezine i remeti pravilno razviće slezine tokom perioda ispitivanja. .",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Body mass, spleen mass and level of thyroid hormones in juvenile hypothyroid rats, Telesna masa, masa slezine i nivo tireoidnih hormona kod juvenilnih hipotireoidnih pacova",
volume = "60",
number = "3-4",
pages = "153-162",
doi = "10.2298/VETGL0604153R"
}

Roksandić, D., Šimić, M., Radovanović, A.,& Gledić, D.. (2006). Body mass, spleen mass and level of thyroid hormones in juvenile hypothyroid rats. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(3-4), 153-162.
https://doi.org/10.2298/VETGL0604153R

Roksandić D, Šimić M, Radovanović A, Gledić D. Body mass, spleen mass and level of thyroid hormones in juvenile hypothyroid rats. in Veterinarski Glasnik. 2006;60(3-4):153-162.
doi:10.2298/VETGL0604153R .

Roksandić, Dragutin, Šimić, Marija, Radovanović, Anita, Gledić, Dušan, "Body mass, spleen mass and level of thyroid hormones in juvenile hypothyroid rats" in Veterinarski Glasnik, 60, no. 3-4 (2006):153-162,
https://doi.org/10.2298/VETGL0604153R . .