TY  - JOUR
AU  - Imatdinov, Almaz R.
AU  - Kazakova, Anna S.
AU  - Šekler, Milanko
AU  - Morozova, Diana Yu
AU  - Lyska, Valentina M.
AU  - Titov, Ilya A.
AU  - Sevskikh, Timofey
AU  - Sereda, Alexey D.
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2407
AB  - Three recombinant plasmid constructs, expressing chimeric proteins containing human ubiquitin fused to an ectodomain of one of the potentially protective proteins (p30, p54 and CD2v) of the attenuated MK-200 strain of African swine fever virus (ASFV), were created as potential inductors of specific antiviral cellular immunity. Three-time immunization of pigs with the mixture of these plasmids led to the formation of virus-specific cytotoxic T-lymphocytes (CTL), but did not induce production of virus-specific antibodies. After challenge with the homologous parental virulent ASFV strain M-78 at a dose of 103 HAD50, all five animals (four immunized pigs and one naïve) fell between the 4th and 7th days post infection. The obtained results demonstrated that induction of CTL did not protect pigs against challenge with the virulent ASFV. Balanced activation of CTL and antibody-mediated cellular mechanisms should be investigated.
AB  - Kao potencijalni induktori specifičnog antivirusnog celularnog imuniteta, kreirane su 3 rekombinantne plazmidne konstrukcije, koje predstavljaju himerne proteine, koje sadrže humani ubikvitin, na čijem ektodomenu je inkorporiran po jedan od sledećih potencijalno zaštićenih proteina (p30, p54 i CD2v), a koji potiču od atenuiranog soja afričke kuge svinja MK - 200. Navedenim plazmidima je izvršena trostruka imunizacija svinja, koja je kod njih dovela do posledičnog formiranja virus specifičnih citotoksičnih T limfocita (CTL), ali bez uticaja na indukciju sinteze virus specifičnih antitela. Nakon veštačke infekcije ovih imunizovanih svinja i to homolognim virulentnim sojem virusa afričke kuge svinja M - 78, u dozi od 103  HAD50, svih 5 svinja (4 imunizovane svinje i 1 neimunizovana - kontrolna) su uginule između 4 i 7 dana nakon infi ciranja. Dobijeni rezultati su pokazali da indukcija samo celularnog imunog odgovora, odnosno citotoksičnih T limfocita, ne štiti svinje od infekcije virulentnim sojem virusa afričke kuge svinja. Izbalansiranu aktivaciju citotoksičnih T limfocita i antitelima posredovane ćelijske imunske mehanizme trebalo bi dalje istražiti.
T2  - Acta Veterinaria-Beograd
T1  - Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus
T1  - Imunizacija svinja sa rekombinantnim plazmidima koji sadrže gene ubikvitarnih p30, p54 i cd2v proteina virusa afričke kuge svinja
VL  - 70
IS  - 1
SP  - 92
EP  - 109
DO  - 10.2478/acve-2020-0007
ER  - 

@article{
author = "Imatdinov, Almaz R. and Kazakova, Anna S. and Šekler, Milanko and Morozova, Diana Yu and Lyska, Valentina M. and Titov, Ilya A. and Sevskikh, Timofey and Sereda, Alexey D.",
year = "2020",
abstract = "Three recombinant plasmid constructs, expressing chimeric proteins containing human ubiquitin fused to an ectodomain of one of the potentially protective proteins (p30, p54 and CD2v) of the attenuated MK-200 strain of African swine fever virus (ASFV), were created as potential inductors of specific antiviral cellular immunity. Three-time immunization of pigs with the mixture of these plasmids led to the formation of virus-specific cytotoxic T-lymphocytes (CTL), but did not induce production of virus-specific antibodies. After challenge with the homologous parental virulent ASFV strain M-78 at a dose of 103 HAD50, all five animals (four immunized pigs and one naïve) fell between the 4th and 7th days post infection. The obtained results demonstrated that induction of CTL did not protect pigs against challenge with the virulent ASFV. Balanced activation of CTL and antibody-mediated cellular mechanisms should be investigated., Kao potencijalni induktori specifičnog antivirusnog celularnog imuniteta, kreirane su 3 rekombinantne plazmidne konstrukcije, koje predstavljaju himerne proteine, koje sadrže humani ubikvitin, na čijem ektodomenu je inkorporiran po jedan od sledećih potencijalno zaštićenih proteina (p30, p54 i CD2v), a koji potiču od atenuiranog soja afričke kuge svinja MK - 200. Navedenim plazmidima je izvršena trostruka imunizacija svinja, koja je kod njih dovela do posledičnog formiranja virus specifičnih citotoksičnih T limfocita (CTL), ali bez uticaja na indukciju sinteze virus specifičnih antitela. Nakon veštačke infekcije ovih imunizovanih svinja i to homolognim virulentnim sojem virusa afričke kuge svinja M - 78, u dozi od 103  HAD50, svih 5 svinja (4 imunizovane svinje i 1 neimunizovana - kontrolna) su uginule između 4 i 7 dana nakon infi ciranja. Dobijeni rezultati su pokazali da indukcija samo celularnog imunog odgovora, odnosno citotoksičnih T limfocita, ne štiti svinje od infekcije virulentnim sojem virusa afričke kuge svinja. Izbalansiranu aktivaciju citotoksičnih T limfocita i antitelima posredovane ćelijske imunske mehanizme trebalo bi dalje istražiti.",
journal = "Acta Veterinaria-Beograd",
title = "Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus, Imunizacija svinja sa rekombinantnim plazmidima koji sadrže gene ubikvitarnih p30, p54 i cd2v proteina virusa afričke kuge svinja",
volume = "70",
number = "1",
pages = "92-109",
doi = "10.2478/acve-2020-0007"
}

Imatdinov, A. R., Kazakova, A. S., Šekler, M., Morozova, D. Y., Lyska, V. M., Titov, I. A., Sevskikh, T.,& Sereda, A. D.. (2020). Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus. in Acta Veterinaria-Beograd, 70(1), 92-109.
https://doi.org/10.2478/acve-2020-0007

Imatdinov AR, Kazakova AS, Šekler M, Morozova DY, Lyska VM, Titov IA, Sevskikh T, Sereda AD. Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus. in Acta Veterinaria-Beograd. 2020;70(1):92-109.
doi:10.2478/acve-2020-0007 .

Imatdinov, Almaz R., Kazakova, Anna S., Šekler, Milanko, Morozova, Diana Yu, Lyska, Valentina M., Titov, Ilya A., Sevskikh, Timofey, Sereda, Alexey D., "Immunization of Pigs with Recombinant Plasmids Containing Genes of Ubiquitinated p30, p54 and CD2v Proteins of African Swine Fever Virus" in Acta Veterinaria-Beograd, 70, no. 1 (2020):92-109,
https://doi.org/10.2478/acve-2020-0007 . .