TY  - JOUR
AU  - Stojić, Milica
AU  - Fratrić, Natalija
AU  - Kovacić, Marijana
AU  - Ilić, Vesna
AU  - Gvozdić, Dragan
AU  - Savić, Olivera
AU  - Doković, Radojica
AU  - Valčić, Olivera
PY  - 2017
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1519
AB  - Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P<0.001 and P<0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin<10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer
VL  - 67
IS  - 4
SP  - 508
EP  - 524
DO  - 10.1515/acve-2017-0041
ER  - 

@article{
author = "Stojić, Milica and Fratrić, Natalija and Kovacić, Marijana and Ilić, Vesna and Gvozdić, Dragan and Savić, Olivera and Doković, Radojica and Valčić, Olivera",
year = "2017",
abstract = "Failure of passive transfer (FPT) of immunoglobulins (IgG) is associated with increased morbidity and mortality of calves. In this study we evaluated the digital Brix refractometer usefulness for the assessment of FPT. A number of 16 colostrum samples from the first milking (2-3h post-partum) of Holstein-Friesian dairy heifers and 29 blood sera of 3-6 days old calves were analyzed with a digital Brix refractometer. Total proteins were determined by the biuret reaction. Colostral IgG were determined by radial immunodiffusion (RID), and colostral whey and blood serum. globulin (composed almost entirely of IgG) were determined by agarose protein gel electrophoresis (APE) and densitometry. Colostral % Brix score was 25.5 +/- 3.4%. Concentrations of colostrum IgG and colostral whey. globulin were 130 +/- 33 g/L and 100 +/- 24 g/L respectively. The concentration of total proteins in colostral whey was 134 +/- 30 g/L. The correlations between Brix values and the concentrations of IgG determined with RID and the concentrations of. globulin determined with APE were positive and highly significant (P<0.001 and P<0.01). The concentration of serum proteins of new-born calves was 57.75 +/- 11.8 g/L, the concentration of. globulin was 14.4 +/- 7.8 g/L, and the Brix score was 8.6 +/- 1.0%. FPT (serum. globulin<10 g/L) was detected in 34.5% (10/29) calves. Brix score correlated with the concentration of blood serum. globulins in all examined calves. The results have confirmed that digital Brix refractometry allows the producers to use this technique in order to estimate colostral and calf serum IgG, thereby monitoring both colostrum quality and success of passive transfer.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer",
volume = "67",
number = "4",
pages = "508-524",
doi = "10.1515/acve-2017-0041"
}

Stojić, M., Fratrić, N., Kovacić, M., Ilić, V., Gvozdić, D., Savić, O., Doković, R.,& Valčić, O.. (2017). Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 67(4), 508-524.
https://doi.org/10.1515/acve-2017-0041

Stojić M, Fratrić N, Kovacić M, Ilić V, Gvozdić D, Savić O, Doković R, Valčić O. Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer. in Acta Veterinaria-Beograd. 2017;67(4):508-524.
doi:10.1515/acve-2017-0041 .

Stojić, Milica, Fratrić, Natalija, Kovacić, Marijana, Ilić, Vesna, Gvozdić, Dragan, Savić, Olivera, Doković, Radojica, Valčić, Olivera, "Brix refractometry of colostrum from primiparous dairy cows and new-born calf blood serum in the evaluation of failure of passive transfer" in Acta Veterinaria-Beograd, 67, no. 4 (2017):508-524,
https://doi.org/10.1515/acve-2017-0041 . .