TY  - JOUR
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Polaček, Vladimir
AU  - Vasković, Nikola
AU  - Ašanin, Ružica
AU  - Milić, Nenad
AU  - Nišavić, Jakov
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/945
AB  - Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from four samples. The identification of isolated strains was done by using the hemagglutination and hemagglutination-inhibition tests. The nucleic acid of the ND virus was identified in all the four samples It was confirmed that all the isolated strains were velogenic strains. Analysis of the nucleotide sequences of the gene encoding the F cleavage site of the fusion F protein showed the presence of motifs 112RRQKRFIG119, characteristic for the velogenic strains of the ND virus. Phylogenetic analysis of the F gene sequences revealed that all isolated strains of the virus belong to class II and genotype VIId.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Application of standard and molecular methods for the diagnosis of Newcastle disease
VL  - 64
IS  - 4
SP  - 1433
EP  - 1437
DO  - 10.2298/ABS1204433V
ER  - 

@article{
author = "Vidanović, Dejan and Šekler, Milanko and Polaček, Vladimir and Vasković, Nikola and Ašanin, Ružica and Milić, Nenad and Nišavić, Jakov",
year = "2012",
abstract = "Four pooled samples of whole poultry carcasses with their internal organs were used to determine the presence of Newcastle disease (ND) virus. Samples were collected from one epizootiological area in the Republic of Serbia during January 2007. Newcastle disease virus strains were isolated from four samples. The identification of isolated strains was done by using the hemagglutination and hemagglutination-inhibition tests. The nucleic acid of the ND virus was identified in all the four samples It was confirmed that all the isolated strains were velogenic strains. Analysis of the nucleotide sequences of the gene encoding the F cleavage site of the fusion F protein showed the presence of motifs 112RRQKRFIG119, characteristic for the velogenic strains of the ND virus. Phylogenetic analysis of the F gene sequences revealed that all isolated strains of the virus belong to class II and genotype VIId.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Application of standard and molecular methods for the diagnosis of Newcastle disease",
volume = "64",
number = "4",
pages = "1433-1437",
doi = "10.2298/ABS1204433V"
}

Vidanović, D., Šekler, M., Polaček, V., Vasković, N., Ašanin, R., Milić, N.,& Nišavić, J.. (2012). Application of standard and molecular methods for the diagnosis of Newcastle disease. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 64(4), 1433-1437.
https://doi.org/10.2298/ABS1204433V

Vidanović D, Šekler M, Polaček V, Vasković N, Ašanin R, Milić N, Nišavić J. Application of standard and molecular methods for the diagnosis of Newcastle disease. in Archives of Biological Sciences. 2012;64(4):1433-1437.
doi:10.2298/ABS1204433V .

Vidanović, Dejan, Šekler, Milanko, Polaček, Vladimir, Vasković, Nikola, Ašanin, Ružica, Milić, Nenad, Nišavić, Jakov, "Application of standard and molecular methods for the diagnosis of Newcastle disease" in Archives of Biological Sciences, 64, no. 4 (2012):1433-1437,
https://doi.org/10.2298/ABS1204433V . .

TY  - JOUR
AU  - Ašanin, Jelena
AU  - Aksentijević, Ksenija
AU  - Žutić, Milenko
AU  - Katić, Vera
AU  - Krnjaić, Dejan
AU  - Milić, Nenad
AU  - Ašanin, Ružica
AU  - Mišić, Dušan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/854
AB  - The objective of this work was to identify isolated Staphylococcus species and to investigate their sensitivity to some antibacterial drugs. The material used for these investigations were Staphylococcus isolates originating from milk samples. A total of 25 strains of Staphylococcus isolates were examined, including 24 from milk samples from cows with mastitis, and one strain was isolated from a milk sample from a cow following treatment for mastitis. For primary identification, catalase and oxidase tests were used, as well as the free coagulase test. Following the preliminary tests, the isolated strains were identified using commercial systems ID32 STAPH (bioMérieux, France) and the BBL Crystal Gram-Positive ID Kit (Becton Dickinson, USA) according to the enclosed instructions. The Staphylococcus isolates were examined for sensitivity to the following: oxacillin, penicillin, cefoxitin, gentamicin, erythromycin, chloramphenicol, tetracycline, ciprofloxacin, sulfametoxazol/trimetoprim, and vacomycin using the disk diffusion method and the broth microdilution method as recommended by the Clinical and Laboratory Strandards Institute - CLSI(2003), and the results were interpreted according to CLSI recommendations from 2008 and 2010. Antibiogram disks manufactured by Becton Dickinson (USA) were used, and the broth microdilution method was applied using pure antibiotic substances from different manufacturers: erythromycin, chloramphenicol, cefoxitin, gentamicin, oxacillin, tetracycline (Sigma Aldrich, USA), sulfametoxazol (Fluka, USA), penicillin (Calbiochem, Germany), vancomycin (Abbott laboratories, USA), ciprofloxacin and trimetoprim (Zdravlje A.D., Serbia). All 25 strains were catalase positive and oxidase negative. Of the 25 strains, 19 were coagulase positive and 6 were coagulase negative.With the implementation of the disk diffusion method on 19 strains of S. aureus, 17 were established to be resistant to penicillin (89.5%), and 2 strains to gentamicin (10.5%). The investigation of 3 strains of S. xylosus using the disk diffusion method showed that one strain was resistant to tetracycline (33.3%) and to oxacillin (33.3%), while another strain was found to be resistant to penicillin (33.3%). The third strain of S. xylosus was sensitive to all the examined antibiotics. Two strains of S. simulans and one strain of S. haemolyticus were not found to be resistant to any of the examined antibiotics using the disk diffusion method. The implementation of the broth microdilution method yielded in 13 strains of S. aureus resistance to penicillin (68.4%) with MIC values from 0.5 to 4 μg/m, in 2 strains to gentamicin (10.5%) with MIC values of 32 μg/ml, and intermediary sensitivity to chloramphenicol was established in 9 strains of S. aureus (47.4%) with MIC values of 16 μg/ml and to vancomycin in 1 strain of S. aureus (5.3%) with MIC values of 4 μg/ml.
AB  - Cilj ovog rada je bila identifikacija izolovanih vrsta stafilokoka i ispitivanje njihove osetljivosti na neke antibakterijske lekove. Kao materijal u ovom ispitivanju korišćeni su izolati stafilokoka poreklom iz uzoraka mleka. Ukupno je ispitano 25 sojeva izolovanih stafilokoka od kojih su 24 poticala iz uzoraka mleka krava sa mastitisom, a jedan soj je izolovan iz uzorka mleka krave nakon lečenja mastitisa. U primarnoj identifikaciji su korišćeni katalaza i oksidaza testovi, kao i test prisustva slobodne koagulaze. Nakon izvođenja preliminarnih testova, vršena je identifikacija izolovanih sojeva, primenom komercijalnih sistema ID32 STAPH (bioMérieux, Francuska) i BBL Crystal Gram-Positive ID Kit (Becton Dickinson, SAD) prema uputstvima proizvođača. Osetljivost izolovanih sojeva stafilokoka ispitivana je na: oksacilin, penicilin, cefoksitin, gentamicin, eritromicin, hloramfenikol, tetraciklin, ciprofloksacin, sulfametoksazol/trimetoprim i vankomicin primenom disk difuzione metode i mikrodilucione metode u bujonu prema preporukama Instituta za kliničke i laboratorijske standarde (Clinical and Laboratory Strandards Institute - CLSI (2003), a tumačenje rezultata je vršeno prema preporukama CLSI iz 2008. i 2010. godine. Korišćeni su antibiogram diskovi proizvođača Becton Dickinson (SAD), a za mikrodilucionu metodu u bujonu korišćene su čiste supstance antibakterijskih lekova različitih proizvođača: eritromicin, hloramfenikol, cefoksitin, gentamicin, oksacilin, tetraciklin (Sigma Aldrich, SAD), sulfametoksazol (Fluka, SAD), penicilin (Calbiochem, Nemačka), vankomicin (Abbott laboratories, SAD), ciprofloksacin i trimetoprim (Zdravlje A.D., Srbija). Svih 25 sojeva je bilo katalaza pozitivno i oksidaza negativno. Od 25 sojeva, 19 je bilo koagulaza-pozitivno, a 6 koagulaza-negativno. Primenom disk difuzione metode od 19 sojeva S. aureus kod 17 je utvrđena rezistencija na penicilin (89,5%), a kod 2 soja na gentamicin (10,5%). Od 3 soja S. xylosus, kod jednog je primenom disk difuzione metode utvrđena rezistencija na tetraciklin (33,3%) i na oksacilin (33,3%), dok je kod drugog soja utvrđena rezistencija na penicilin (33,3%). Treći soj S. xylosus je bio osetljiv na sve ispitivane antibiotike. Kod dva soja S. simulans i jednog soja S. haemolyticus nije utvrđena rezistencija ni na jedan od ispitivanih antibiotika primenom disk difuzione metode. Primenom mikrodilucione metode u bujonu kod 13 sojeva S. aureus je utvrđena rezistencija na penicilin (68,4%) sa vrednostima minimalne inhibitorne koncentracije (MIC) od 0,5 do 4 μg/m, kod 2 soja na gentamicin (10,5%) sa vrednostima MIC od 32 μg/ml, a intermedijarna osetljivost na hloramfenikol utvrđena je kod 9 sojeva S. aureus (47,4%) sa vrednostima MIC od 16 μg/ml i na vankomicin kod jednog soja S. aureus (5,3%) čija je vrednost MIC iznosila 4 μg/ml.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution
T1  - Ispitivanje osetljivosti Staphylococcus vrsta na neke antibakterijske lekove primenom disk difuzione i mikrodilucione metode u bujonu
VL  - 66
IS  - 3-4
SP  - 199
EP  - 210
DO  - 10.2298/VETGL1204199A
ER  - 

@article{
author = "Ašanin, Jelena and Aksentijević, Ksenija and Žutić, Milenko and Katić, Vera and Krnjaić, Dejan and Milić, Nenad and Ašanin, Ružica and Mišić, Dušan",
year = "2012",
abstract = "The objective of this work was to identify isolated Staphylococcus species and to investigate their sensitivity to some antibacterial drugs. The material used for these investigations were Staphylococcus isolates originating from milk samples. A total of 25 strains of Staphylococcus isolates were examined, including 24 from milk samples from cows with mastitis, and one strain was isolated from a milk sample from a cow following treatment for mastitis. For primary identification, catalase and oxidase tests were used, as well as the free coagulase test. Following the preliminary tests, the isolated strains were identified using commercial systems ID32 STAPH (bioMérieux, France) and the BBL Crystal Gram-Positive ID Kit (Becton Dickinson, USA) according to the enclosed instructions. The Staphylococcus isolates were examined for sensitivity to the following: oxacillin, penicillin, cefoxitin, gentamicin, erythromycin, chloramphenicol, tetracycline, ciprofloxacin, sulfametoxazol/trimetoprim, and vacomycin using the disk diffusion method and the broth microdilution method as recommended by the Clinical and Laboratory Strandards Institute - CLSI(2003), and the results were interpreted according to CLSI recommendations from 2008 and 2010. Antibiogram disks manufactured by Becton Dickinson (USA) were used, and the broth microdilution method was applied using pure antibiotic substances from different manufacturers: erythromycin, chloramphenicol, cefoxitin, gentamicin, oxacillin, tetracycline (Sigma Aldrich, USA), sulfametoxazol (Fluka, USA), penicillin (Calbiochem, Germany), vancomycin (Abbott laboratories, USA), ciprofloxacin and trimetoprim (Zdravlje A.D., Serbia). All 25 strains were catalase positive and oxidase negative. Of the 25 strains, 19 were coagulase positive and 6 were coagulase negative.With the implementation of the disk diffusion method on 19 strains of S. aureus, 17 were established to be resistant to penicillin (89.5%), and 2 strains to gentamicin (10.5%). The investigation of 3 strains of S. xylosus using the disk diffusion method showed that one strain was resistant to tetracycline (33.3%) and to oxacillin (33.3%), while another strain was found to be resistant to penicillin (33.3%). The third strain of S. xylosus was sensitive to all the examined antibiotics. Two strains of S. simulans and one strain of S. haemolyticus were not found to be resistant to any of the examined antibiotics using the disk diffusion method. The implementation of the broth microdilution method yielded in 13 strains of S. aureus resistance to penicillin (68.4%) with MIC values from 0.5 to 4 μg/m, in 2 strains to gentamicin (10.5%) with MIC values of 32 μg/ml, and intermediary sensitivity to chloramphenicol was established in 9 strains of S. aureus (47.4%) with MIC values of 16 μg/ml and to vancomycin in 1 strain of S. aureus (5.3%) with MIC values of 4 μg/ml., Cilj ovog rada je bila identifikacija izolovanih vrsta stafilokoka i ispitivanje njihove osetljivosti na neke antibakterijske lekove. Kao materijal u ovom ispitivanju korišćeni su izolati stafilokoka poreklom iz uzoraka mleka. Ukupno je ispitano 25 sojeva izolovanih stafilokoka od kojih su 24 poticala iz uzoraka mleka krava sa mastitisom, a jedan soj je izolovan iz uzorka mleka krave nakon lečenja mastitisa. U primarnoj identifikaciji su korišćeni katalaza i oksidaza testovi, kao i test prisustva slobodne koagulaze. Nakon izvođenja preliminarnih testova, vršena je identifikacija izolovanih sojeva, primenom komercijalnih sistema ID32 STAPH (bioMérieux, Francuska) i BBL Crystal Gram-Positive ID Kit (Becton Dickinson, SAD) prema uputstvima proizvođača. Osetljivost izolovanih sojeva stafilokoka ispitivana je na: oksacilin, penicilin, cefoksitin, gentamicin, eritromicin, hloramfenikol, tetraciklin, ciprofloksacin, sulfametoksazol/trimetoprim i vankomicin primenom disk difuzione metode i mikrodilucione metode u bujonu prema preporukama Instituta za kliničke i laboratorijske standarde (Clinical and Laboratory Strandards Institute - CLSI (2003), a tumačenje rezultata je vršeno prema preporukama CLSI iz 2008. i 2010. godine. Korišćeni su antibiogram diskovi proizvođača Becton Dickinson (SAD), a za mikrodilucionu metodu u bujonu korišćene su čiste supstance antibakterijskih lekova različitih proizvođača: eritromicin, hloramfenikol, cefoksitin, gentamicin, oksacilin, tetraciklin (Sigma Aldrich, SAD), sulfametoksazol (Fluka, SAD), penicilin (Calbiochem, Nemačka), vankomicin (Abbott laboratories, SAD), ciprofloksacin i trimetoprim (Zdravlje A.D., Srbija). Svih 25 sojeva je bilo katalaza pozitivno i oksidaza negativno. Od 25 sojeva, 19 je bilo koagulaza-pozitivno, a 6 koagulaza-negativno. Primenom disk difuzione metode od 19 sojeva S. aureus kod 17 je utvrđena rezistencija na penicilin (89,5%), a kod 2 soja na gentamicin (10,5%). Od 3 soja S. xylosus, kod jednog je primenom disk difuzione metode utvrđena rezistencija na tetraciklin (33,3%) i na oksacilin (33,3%), dok je kod drugog soja utvrđena rezistencija na penicilin (33,3%). Treći soj S. xylosus je bio osetljiv na sve ispitivane antibiotike. Kod dva soja S. simulans i jednog soja S. haemolyticus nije utvrđena rezistencija ni na jedan od ispitivanih antibiotika primenom disk difuzione metode. Primenom mikrodilucione metode u bujonu kod 13 sojeva S. aureus je utvrđena rezistencija na penicilin (68,4%) sa vrednostima minimalne inhibitorne koncentracije (MIC) od 0,5 do 4 μg/m, kod 2 soja na gentamicin (10,5%) sa vrednostima MIC od 32 μg/ml, a intermedijarna osetljivost na hloramfenikol utvrđena je kod 9 sojeva S. aureus (47,4%) sa vrednostima MIC od 16 μg/ml i na vankomicin kod jednog soja S. aureus (5,3%) čija je vrednost MIC iznosila 4 μg/ml.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution, Ispitivanje osetljivosti Staphylococcus vrsta na neke antibakterijske lekove primenom disk difuzione i mikrodilucione metode u bujonu",
volume = "66",
number = "3-4",
pages = "199-210",
doi = "10.2298/VETGL1204199A"
}

Ašanin, J., Aksentijević, K., Žutić, M., Katić, V., Krnjaić, D., Milić, N., Ašanin, R.,& Mišić, D.. (2012). Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 66(3-4), 199-210.
https://doi.org/10.2298/VETGL1204199A

Ašanin J, Aksentijević K, Žutić M, Katić V, Krnjaić D, Milić N, Ašanin R, Mišić D. Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution. in Veterinarski Glasnik. 2012;66(3-4):199-210.
doi:10.2298/VETGL1204199A .

Ašanin, Jelena, Aksentijević, Ksenija, Žutić, Milenko, Katić, Vera, Krnjaić, Dejan, Milić, Nenad, Ašanin, Ružica, Mišić, Dušan, "Investigation of susceptibility of Staphylococcus species to some antibacterial drugs by disk diffusion and broth microdilution" in Veterinarski Glasnik, 66, no. 3-4 (2012):199-210,
https://doi.org/10.2298/VETGL1204199A . .

TY  - JOUR
AU  - Ašanin, Jelena
AU  - Aksentijević, Ksenija
AU  - Zdravković, Nemanja
AU  - Ašanin, Ružica
AU  - Mišić, Dušan
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/850
AB  - For the purpose of detecting methicillin (oxacillin) resistance in staphylococcal strains, in a number of microbiological laboratories only disc diffusion method with cefoxitin and/or oxacillin discs is used. Besides this method, it is desirable to determine MIC values for cefoxitin and/or oxacillin. After examination by disc diffusion and dilution methods, latex agglutination is used for the detection of PBP2a and PCR is used for the detection of mecA gene. Use of PCR is not possible in a large number of diagnostic laboratories and as method of choice, latex agglutination test for rapid detection of PBP2a is recommended. In this investigation, as confirmatory methods, latex agglutination and PCR were used for strains that were resistant to oxacillin and/or cefoxitin by disc diffusion and broth microdilution methods. In total, 14 strains of coagulase-negative staphylococci originating from clinical specimens of cats, dogs and chicken were examined. Among isolated strains, it was established that the dominating species was Staphylococcus haemolyticus with 11 isolated strains. Other isolated species were Staphylococcus epidermidis, Staphylococcus capitis and Staphylococcus vitulinus, each with one isolated strain. For all 14 strains, oxacillin MIC values ranged from 0.5 μg/mL to >64 μg/mL and cefoxitin MIC values ranged from 1 μg/mL to >256 μg/mL. Positive agglutination reaction by latex agglutination test was recorded in 13 out of 14 strains. The PCR assay for mecA gene was positive in 12 investigated strains.
AB  - Radi otkrivanja meticilin (oksacilin)-rezistentnih sojeva stafilokoka u većini mikrobioloških laboratorija koristi se najčešće samo disk difuziona metoda uz primenu antibiogram diskova oksacilina i cefoksitina. Pored navedene metode, poželjno je da se utvrde i vrednosti MIC oksacilina i/ili cefoksitina primenom dilucione metode u bujonu ili agaru. Nakon ispitivanja pomenutim metodama za definitivno utvrđivanje pripadnosti soja grupi meticilin rezistentnih stafilokoka, koriste se metoda lateks aglutinacije za utvrđivanje prisustva PBP2a i PCR metoda za detekciju mecA gena. Kako primena metode PCR nije moguća u većini laboratorija koje se bave rutinskom dijagnostikom, kao metoda izbora preporučuje se lateks aglutinacioni test za brzo otkrivanje PBP2a. U ovom ispitivanju primenjeni su lateks aglutinacioni test i PCR metod, kao potvrdne metode za ispitivanje sojeva stafilokoka koji su bili rezistentni na oksacilin i/ili cefoksitin na osnovu rezultata dobijenih disk difuzionom metodom i mikrodilucionom metodom u bujonu. Ukupno je ispitano 14 sojeva koagulaza - negativnih stafilokoka izolovanih iz kliničkih uzoraka poreklom od pasa, mačaka i kokoši. Među izolovanim sojevima, ustanovljeno je da dominira vrsta Staphylococcus haemolyticus sa 11 izolata, a po jedan soj je izolovan od vrsta Staphylococcus epidermidis, Staphylococcus capitis i Staphylococcus vitulinus . Vrednosti MIC oksacilina za 14 sojeva iznosile su od 0.5 mg/mL do >64 mg/mL, a vrednosti MIC cefoksitina iznosile su od 1 mg/mL do >256 mg/mL. Kod 13 od 14 sojeva utvrđena je pozitivna reakcija aglutinacije primenom lateks aglutinacionog testa. Kod 12 ispitivanih sojeva je utvrđeno prisustvo mecA gena PCR metodom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals
T1  - Otkrivanje prisustva PBP2a (Penicillin-binding proteina 2a) i mecA gena kod meticilin rezistentnih Stafilokoka poreklom od životinja
VL  - 62
IS  - 4
SP  - 375
EP  - 384
DO  - 10.2298/AVB1204375A
ER  - 

@article{
author = "Ašanin, Jelena and Aksentijević, Ksenija and Zdravković, Nemanja and Ašanin, Ružica and Mišić, Dušan",
year = "2012",
abstract = "For the purpose of detecting methicillin (oxacillin) resistance in staphylococcal strains, in a number of microbiological laboratories only disc diffusion method with cefoxitin and/or oxacillin discs is used. Besides this method, it is desirable to determine MIC values for cefoxitin and/or oxacillin. After examination by disc diffusion and dilution methods, latex agglutination is used for the detection of PBP2a and PCR is used for the detection of mecA gene. Use of PCR is not possible in a large number of diagnostic laboratories and as method of choice, latex agglutination test for rapid detection of PBP2a is recommended. In this investigation, as confirmatory methods, latex agglutination and PCR were used for strains that were resistant to oxacillin and/or cefoxitin by disc diffusion and broth microdilution methods. In total, 14 strains of coagulase-negative staphylococci originating from clinical specimens of cats, dogs and chicken were examined. Among isolated strains, it was established that the dominating species was Staphylococcus haemolyticus with 11 isolated strains. Other isolated species were Staphylococcus epidermidis, Staphylococcus capitis and Staphylococcus vitulinus, each with one isolated strain. For all 14 strains, oxacillin MIC values ranged from 0.5 μg/mL to >64 μg/mL and cefoxitin MIC values ranged from 1 μg/mL to >256 μg/mL. Positive agglutination reaction by latex agglutination test was recorded in 13 out of 14 strains. The PCR assay for mecA gene was positive in 12 investigated strains., Radi otkrivanja meticilin (oksacilin)-rezistentnih sojeva stafilokoka u većini mikrobioloških laboratorija koristi se najčešće samo disk difuziona metoda uz primenu antibiogram diskova oksacilina i cefoksitina. Pored navedene metode, poželjno je da se utvrde i vrednosti MIC oksacilina i/ili cefoksitina primenom dilucione metode u bujonu ili agaru. Nakon ispitivanja pomenutim metodama za definitivno utvrđivanje pripadnosti soja grupi meticilin rezistentnih stafilokoka, koriste se metoda lateks aglutinacije za utvrđivanje prisustva PBP2a i PCR metoda za detekciju mecA gena. Kako primena metode PCR nije moguća u većini laboratorija koje se bave rutinskom dijagnostikom, kao metoda izbora preporučuje se lateks aglutinacioni test za brzo otkrivanje PBP2a. U ovom ispitivanju primenjeni su lateks aglutinacioni test i PCR metod, kao potvrdne metode za ispitivanje sojeva stafilokoka koji su bili rezistentni na oksacilin i/ili cefoksitin na osnovu rezultata dobijenih disk difuzionom metodom i mikrodilucionom metodom u bujonu. Ukupno je ispitano 14 sojeva koagulaza - negativnih stafilokoka izolovanih iz kliničkih uzoraka poreklom od pasa, mačaka i kokoši. Među izolovanim sojevima, ustanovljeno je da dominira vrsta Staphylococcus haemolyticus sa 11 izolata, a po jedan soj je izolovan od vrsta Staphylococcus epidermidis, Staphylococcus capitis i Staphylococcus vitulinus . Vrednosti MIC oksacilina za 14 sojeva iznosile su od 0.5 mg/mL do >64 mg/mL, a vrednosti MIC cefoksitina iznosile su od 1 mg/mL do >256 mg/mL. Kod 13 od 14 sojeva utvrđena je pozitivna reakcija aglutinacije primenom lateks aglutinacionog testa. Kod 12 ispitivanih sojeva je utvrđeno prisustvo mecA gena PCR metodom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals, Otkrivanje prisustva PBP2a (Penicillin-binding proteina 2a) i mecA gena kod meticilin rezistentnih Stafilokoka poreklom od životinja",
volume = "62",
number = "4",
pages = "375-384",
doi = "10.2298/AVB1204375A"
}

Ašanin, J., Aksentijević, K., Zdravković, N., Ašanin, R.,& Mišić, D.. (2012). Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(4), 375-384.
https://doi.org/10.2298/AVB1204375A

Ašanin J, Aksentijević K, Zdravković N, Ašanin R, Mišić D. Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals. in Acta Veterinaria-Beograd. 2012;62(4):375-384.
doi:10.2298/AVB1204375A .

Ašanin, Jelena, Aksentijević, Ksenija, Zdravković, Nemanja, Ašanin, Ružica, Mišić, Dušan, "Detection of PBP2a (Penicillin-binding protein 2a) and mecA gene in methicillin resistant Staphylococci originated from animals" in Acta Veterinaria-Beograd, 62, no. 4 (2012):375-384,
https://doi.org/10.2298/AVB1204375A . .

TY  - JOUR
AU  - Stanojković, Aleksandar
AU  - Ašanin, Ružica
AU  - Mišić, Dušan
AU  - Ašanin, Jelena
AU  - Stanojkovic-Sebić, Aleksandra
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/868
AB  - Streptococcus suis is a normal inhabitant of the respiratory system of pigs. Clinically healthy pigs are a major reservoir of the pathogen, but, as well, the most important link in the epidemiology of infection in humans. Hence, the aim of this study was to establish the presence of S. suis on some pig farms in Serbia and to determine their serotype affiliation. For this research, we tested 226 samples: nasopharyngeal tonsil swabs, nasal cavities swabs, parts of or all nasopharyngeal tonsils, swabs from dead animals, swabs from pig carcasses, and swabs from the butchers knives. Identification of S. suis strains was performed with antisera specific for capsular antigens. S. suis serotype 2 was established in 67.7% cases, and serotypes 7, 9 and 1 were isolated in a much smaller percentage (17.6, 8.8 and 5.9%, respectively). Serotype 2 is the only serotype isolated from all the collected samples. Serotype 9 was isolated from healthy pig tonsil swab and from 2 nasal swabs, and was not found in the samples taken from diseased pigs. The serotype 1 was isolated only from nasopharyngeal tonsil swabs of piglets, while serotype 7 was isolated from nasopharyngeal tonsil swabs, nasopharyngeal tonsil sections, but also from nasal swabs of healthy pigs. It can be concluded that the infections caused by different serotypes of S. suis are present in the herd of pigs in Serbia; hence, it is very important to identify what serotypes of S. suis are involved in pathogenesis of infections in pigs.
PB  - Parlar Scientific Publications (P S P), Freising
T2  - Fresenius Environmental Bulletin
T1  - The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia
VL  - 21
IS  - 11C
SP  - 3558
EP  - 3561
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_868
ER  - 

@article{
author = "Stanojković, Aleksandar and Ašanin, Ružica and Mišić, Dušan and Ašanin, Jelena and Stanojkovic-Sebić, Aleksandra",
year = "2012",
abstract = "Streptococcus suis is a normal inhabitant of the respiratory system of pigs. Clinically healthy pigs are a major reservoir of the pathogen, but, as well, the most important link in the epidemiology of infection in humans. Hence, the aim of this study was to establish the presence of S. suis on some pig farms in Serbia and to determine their serotype affiliation. For this research, we tested 226 samples: nasopharyngeal tonsil swabs, nasal cavities swabs, parts of or all nasopharyngeal tonsils, swabs from dead animals, swabs from pig carcasses, and swabs from the butchers knives. Identification of S. suis strains was performed with antisera specific for capsular antigens. S. suis serotype 2 was established in 67.7% cases, and serotypes 7, 9 and 1 were isolated in a much smaller percentage (17.6, 8.8 and 5.9%, respectively). Serotype 2 is the only serotype isolated from all the collected samples. Serotype 9 was isolated from healthy pig tonsil swab and from 2 nasal swabs, and was not found in the samples taken from diseased pigs. The serotype 1 was isolated only from nasopharyngeal tonsil swabs of piglets, while serotype 7 was isolated from nasopharyngeal tonsil swabs, nasopharyngeal tonsil sections, but also from nasal swabs of healthy pigs. It can be concluded that the infections caused by different serotypes of S. suis are present in the herd of pigs in Serbia; hence, it is very important to identify what serotypes of S. suis are involved in pathogenesis of infections in pigs.",
publisher = "Parlar Scientific Publications (P S P), Freising",
journal = "Fresenius Environmental Bulletin",
title = "The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia",
volume = "21",
number = "11C",
pages = "3558-3561",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_868"
}

Stanojković, A., Ašanin, R., Mišić, D., Ašanin, J.,& Stanojkovic-Sebić, A.. (2012). The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia. in Fresenius Environmental Bulletin
Parlar Scientific Publications (P S P), Freising., 21(11C), 3558-3561.
https://hdl.handle.net/21.15107/rcub_veterinar_868

Stanojković A, Ašanin R, Mišić D, Ašanin J, Stanojkovic-Sebić A. The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia. in Fresenius Environmental Bulletin. 2012;21(11C):3558-3561.
https://hdl.handle.net/21.15107/rcub_veterinar_868 .

Stanojković, Aleksandar, Ašanin, Ružica, Mišić, Dušan, Ašanin, Jelena, Stanojkovic-Sebić, Aleksandra, "The presence and serological types of streptococcus suis strains isolated from pigs originating from some farms in Serbia" in Fresenius Environmental Bulletin, 21, no. 11C (2012):3558-3561,
https://hdl.handle.net/21.15107/rcub_veterinar_868 .

TY  - JOUR
AU  - Kuzmanović, Jelena
AU  - Ašanin, Ružica
AU  - Baltić, Milan Ž.
AU  - Mišić, Dušan
AU  - Dimitrijević, Mirjana
AU  - Stojanović, Marija
AU  - Ašanin, N.
AU  - Kovačević, I.
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/836
AB  - Listeria monocytogenes is a bacterium that is pathogenic for man and for most animal species. Listeriosis is a generalized infection that starts after ingestion of the causative agent L. monocytogenes (Finlay, 2001). Food that is not properly thermically processed, long storage of such food, food that is produced in inadequate hygienic food plants, as well as cooked-cooled ready to eat food (RTE) is the ideal medium for listeria multiplication. High risk food originated from fish, fish products and sea products comprised of: molluscs (fresh of frozen shellfish, crustaceans shelled or not), fresh fish (ready to eat without cooking), fish products packed with brine (NaCl lt 6%), (salted, marinated, fermented, cold smoked and fish in brine), thermically treated fish and crustacean products (pasteurization, cooking, hot smoking, including pre-cooking and panning). In this research, fish samples, fish products and sea products from Serbian markets were examinated such as: fresh fish (cooled), frozen food (fish and sea products - cuttlefish, squid, octopus, shellfish, crustaceans and shrimps), panned products; smoked fish, salted fish, thermally treated fish and fish products, semi-canned fish and canned fish. Microbiological testing has been performed according to internationally prescribed standards ISO 11290-1 (1996) 'Microbiology of food and animal feed stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes'. There were 470 samples from fish products and sea products. Presence of Listeria spp was registered in 58 samples (12.34%). Listeria monocytogenes was found in 1.92% samples (9 isolates), which makes 15.52% of the total Listeria that were isolated from the tested food. Other isolated Listeria belong to the following species: L. innocua (8.51%), L.welshimeri (1.28%), L.welshimeri/innocua (0.21%), L. grayi (0.21%) and L. seeligeri (0.21%). Exceptional viability of Listeria monocytogenes in the food tested was documented, also (freezing temperature - 18oC, five months).
AB  - Listeria monocytogenes je bakterija patogena za ljude i veliki broj životinjskih vrsta. Listerioza predstavlja generalizovanu infekciju koja nastaje nakon oralne ingestije uzročnog agensa L. monocytogenes (Finlay, 2001). Navedena bakterija se najbolje razvija u namirnicama koje nisu adekvatno termički obrađene; koje su dugo skladištene; koje su proizvedene na mestu na kojem principi higijenskog rukovanja hranom nisu sprovođeni; kuvano-hladnoj hrani spremnoj za jelo (ready to eat food - RTE) U visoko rizične namirnice počev od vrsta ribe, proizvoda od ribe i plodova mora ubrajaju se: mekušci (sveže i zamrznute školjke, rakovi u ljušturama, ili očišćeni), sveža riba (koja se konzumira bez termičke obrade), riblji proizvodi u vodenom rastvoru soli (NaCl%), (soljena, marinirana, fermentisana, hladno dimljena i riba u sopstvenom soku), srednje termički obrađeni riblji proizvodi i rakovi (pasterizacija, kuvanje, toplo dimljenje, uključujući i pred-kuvanje i panirani proizvodi). Kao materijal za ispitivanje korišćeni su uzorci riba, proizvoda od riba i morski plodovi: sveža riba (u rashlađenom stanju), zamrznute namirnice (riba i morski plodovi - lignje, sipe, hobotnice, školjke, rakovi i škampi), panirani proizvodi, dimljena riba, usoljena riba, termički obrađena riba i proizvodi od ribe, polukonzerve i konzerve od ribe. Mikrobiološko ispitivanje je rađeno prema propisanoj metodi po Međunarodnom standardu ISO 11290-1 (1996) 'Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes'. Ukupno je ispitano 470 uzoraka ribe, proizvoda od ribe i morskih plodova. Prisustvo Listeria vrsta utvrđeno je u 58 uzoraka (12,34%). Listeria monocytogenes je utvrđena kod 1,92% pregledanih uzoraka (9 izolata) i čini 15,52% od svih vrsta iz roda Listeria koje su izolovane iz navedenih namirnica. Ostale vrste listerija koje su izolovane pripadale su: L. innocua (8,51%), L. welshimeri (1,28%), L. welshimeri/innocua (0,21%), L. grayi (0,21%) i L. seeligeri (0,21%). Ustanovljena je izuzetna sposobnost preživljavanja pri niskim temperaturama vrste Listeria monocytogenes u namirnicama koje su čuvane pri temperaturi od -18oC tokom 5 meseci.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Presence of Listeria spp. in fish samples, fish products and sea products
T1  - Prisustvo Listerija vrsta u uzorcima riba, proizvoda od ribe i morskih plodova
VL  - 61
IS  - 2-3
SP  - 193
EP  - 203
DO  - 10.2298/AVB1103193K
ER  - 

@article{
author = "Kuzmanović, Jelena and Ašanin, Ružica and Baltić, Milan Ž. and Mišić, Dušan and Dimitrijević, Mirjana and Stojanović, Marija and Ašanin, N. and Kovačević, I.",
year = "2011",
abstract = "Listeria monocytogenes is a bacterium that is pathogenic for man and for most animal species. Listeriosis is a generalized infection that starts after ingestion of the causative agent L. monocytogenes (Finlay, 2001). Food that is not properly thermically processed, long storage of such food, food that is produced in inadequate hygienic food plants, as well as cooked-cooled ready to eat food (RTE) is the ideal medium for listeria multiplication. High risk food originated from fish, fish products and sea products comprised of: molluscs (fresh of frozen shellfish, crustaceans shelled or not), fresh fish (ready to eat without cooking), fish products packed with brine (NaCl lt 6%), (salted, marinated, fermented, cold smoked and fish in brine), thermically treated fish and crustacean products (pasteurization, cooking, hot smoking, including pre-cooking and panning). In this research, fish samples, fish products and sea products from Serbian markets were examinated such as: fresh fish (cooled), frozen food (fish and sea products - cuttlefish, squid, octopus, shellfish, crustaceans and shrimps), panned products; smoked fish, salted fish, thermally treated fish and fish products, semi-canned fish and canned fish. Microbiological testing has been performed according to internationally prescribed standards ISO 11290-1 (1996) 'Microbiology of food and animal feed stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes'. There were 470 samples from fish products and sea products. Presence of Listeria spp was registered in 58 samples (12.34%). Listeria monocytogenes was found in 1.92% samples (9 isolates), which makes 15.52% of the total Listeria that were isolated from the tested food. Other isolated Listeria belong to the following species: L. innocua (8.51%), L.welshimeri (1.28%), L.welshimeri/innocua (0.21%), L. grayi (0.21%) and L. seeligeri (0.21%). Exceptional viability of Listeria monocytogenes in the food tested was documented, also (freezing temperature - 18oC, five months)., Listeria monocytogenes je bakterija patogena za ljude i veliki broj životinjskih vrsta. Listerioza predstavlja generalizovanu infekciju koja nastaje nakon oralne ingestije uzročnog agensa L. monocytogenes (Finlay, 2001). Navedena bakterija se najbolje razvija u namirnicama koje nisu adekvatno termički obrađene; koje su dugo skladištene; koje su proizvedene na mestu na kojem principi higijenskog rukovanja hranom nisu sprovođeni; kuvano-hladnoj hrani spremnoj za jelo (ready to eat food - RTE) U visoko rizične namirnice počev od vrsta ribe, proizvoda od ribe i plodova mora ubrajaju se: mekušci (sveže i zamrznute školjke, rakovi u ljušturama, ili očišćeni), sveža riba (koja se konzumira bez termičke obrade), riblji proizvodi u vodenom rastvoru soli (NaCl%), (soljena, marinirana, fermentisana, hladno dimljena i riba u sopstvenom soku), srednje termički obrađeni riblji proizvodi i rakovi (pasterizacija, kuvanje, toplo dimljenje, uključujući i pred-kuvanje i panirani proizvodi). Kao materijal za ispitivanje korišćeni su uzorci riba, proizvoda od riba i morski plodovi: sveža riba (u rashlađenom stanju), zamrznute namirnice (riba i morski plodovi - lignje, sipe, hobotnice, školjke, rakovi i škampi), panirani proizvodi, dimljena riba, usoljena riba, termički obrađena riba i proizvodi od ribe, polukonzerve i konzerve od ribe. Mikrobiološko ispitivanje je rađeno prema propisanoj metodi po Međunarodnom standardu ISO 11290-1 (1996) 'Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of Listeria monocytogenes'. Ukupno je ispitano 470 uzoraka ribe, proizvoda od ribe i morskih plodova. Prisustvo Listeria vrsta utvrđeno je u 58 uzoraka (12,34%). Listeria monocytogenes je utvrđena kod 1,92% pregledanih uzoraka (9 izolata) i čini 15,52% od svih vrsta iz roda Listeria koje su izolovane iz navedenih namirnica. Ostale vrste listerija koje su izolovane pripadale su: L. innocua (8,51%), L. welshimeri (1,28%), L. welshimeri/innocua (0,21%), L. grayi (0,21%) i L. seeligeri (0,21%). Ustanovljena je izuzetna sposobnost preživljavanja pri niskim temperaturama vrste Listeria monocytogenes u namirnicama koje su čuvane pri temperaturi od -18oC tokom 5 meseci.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Presence of Listeria spp. in fish samples, fish products and sea products, Prisustvo Listerija vrsta u uzorcima riba, proizvoda od ribe i morskih plodova",
volume = "61",
number = "2-3",
pages = "193-203",
doi = "10.2298/AVB1103193K"
}

Kuzmanović, J., Ašanin, R., Baltić, M. Ž., Mišić, D., Dimitrijević, M., Stojanović, M., Ašanin, N.,& Kovačević, I.. (2011). Presence of Listeria spp. in fish samples, fish products and sea products. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(2-3), 193-203.
https://doi.org/10.2298/AVB1103193K

Kuzmanović J, Ašanin R, Baltić MŽ, Mišić D, Dimitrijević M, Stojanović M, Ašanin N, Kovačević I. Presence of Listeria spp. in fish samples, fish products and sea products. in Acta Veterinaria-Beograd. 2011;61(2-3):193-203.
doi:10.2298/AVB1103193K .

Kuzmanović, Jelena, Ašanin, Ružica, Baltić, Milan Ž., Mišić, Dušan, Dimitrijević, Mirjana, Stojanović, Marija, Ašanin, N., Kovačević, I., "Presence of Listeria spp. in fish samples, fish products and sea products" in Acta Veterinaria-Beograd, 61, no. 2-3 (2011):193-203,
https://doi.org/10.2298/AVB1103193K . .

TY  - JOUR
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
AU  - Ašanin, Ružica
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Petrović, Tamaš
AU  - Savić, Vladimir
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/794
AB  - Avian paramyxoviruses type 1 or Newcastle disease viruses (NDV) are frequently recovered from wild birds and such isolates are most frequently of low virulence. Velogenic NDV are usually recovered from poultry and only occasionally from wild birds. Five NDV isolates were obtained from carcasses of four wild bird species during 2007 in Serbia: Mallard (Anas platyrhynchos), Eurasian Sparrowhawk (Accipiter nisus), feral Rock Pigeon (Columba livia), and Eurasian Collared Dove (Streptopelia decaocto). All the isolates have a typical fusion protein cleavage site motif of velogenic viruses (R-112-R-Q-K-R-F-117). The highest homology (99%) for the nucleotide sequences spanning the M and F gene of the studied isolates was with the genotype VII NDV isolate Muscovy duck/China(Fujian)/FP1/02. Phylogenetic analysis based on a partial F gene sequence showed that the isolates from wild birds cluster together with concurrent isolates from poultry in Serbia within the subgenotype VIId, which is the predominant pathogen involved currently in Newcastle disease outbreaks in poultry worldwide. It is unlikely that the wild birds played an important role in primary introduction or consequent spread of the velogenic NDV to domestic poultry in Serbia, and they probably contracted the virus from locally infected poultry.
PB  - Wildlife Disease Assoc, Inc, Lawrence
T2  - Journal of Wildlife Diseases
T1  - Characterization of velogenic newcastle disease viruses isolated from dead wild birds in Serbia during 2007
VL  - 47
IS  - 2
SP  - 433
EP  - 441
DO  - 10.7589/0090-3558-47.2.433
ER  - 

@article{
author = "Vidanović, Dejan and Šekler, Milanko and Ašanin, Ružica and Milić, Nenad and Nišavić, Jakov and Petrović, Tamaš and Savić, Vladimir",
year = "2011",
abstract = "Avian paramyxoviruses type 1 or Newcastle disease viruses (NDV) are frequently recovered from wild birds and such isolates are most frequently of low virulence. Velogenic NDV are usually recovered from poultry and only occasionally from wild birds. Five NDV isolates were obtained from carcasses of four wild bird species during 2007 in Serbia: Mallard (Anas platyrhynchos), Eurasian Sparrowhawk (Accipiter nisus), feral Rock Pigeon (Columba livia), and Eurasian Collared Dove (Streptopelia decaocto). All the isolates have a typical fusion protein cleavage site motif of velogenic viruses (R-112-R-Q-K-R-F-117). The highest homology (99%) for the nucleotide sequences spanning the M and F gene of the studied isolates was with the genotype VII NDV isolate Muscovy duck/China(Fujian)/FP1/02. Phylogenetic analysis based on a partial F gene sequence showed that the isolates from wild birds cluster together with concurrent isolates from poultry in Serbia within the subgenotype VIId, which is the predominant pathogen involved currently in Newcastle disease outbreaks in poultry worldwide. It is unlikely that the wild birds played an important role in primary introduction or consequent spread of the velogenic NDV to domestic poultry in Serbia, and they probably contracted the virus from locally infected poultry.",
publisher = "Wildlife Disease Assoc, Inc, Lawrence",
journal = "Journal of Wildlife Diseases",
title = "Characterization of velogenic newcastle disease viruses isolated from dead wild birds in Serbia during 2007",
volume = "47",
number = "2",
pages = "433-441",
doi = "10.7589/0090-3558-47.2.433"
}

Vidanović, D., Šekler, M., Ašanin, R., Milić, N., Nišavić, J., Petrović, T.,& Savić, V.. (2011). Characterization of velogenic newcastle disease viruses isolated from dead wild birds in Serbia during 2007. in Journal of Wildlife Diseases
Wildlife Disease Assoc, Inc, Lawrence., 47(2), 433-441.
https://doi.org/10.7589/0090-3558-47.2.433

Vidanović D, Šekler M, Ašanin R, Milić N, Nišavić J, Petrović T, Savić V. Characterization of velogenic newcastle disease viruses isolated from dead wild birds in Serbia during 2007. in Journal of Wildlife Diseases. 2011;47(2):433-441.
doi:10.7589/0090-3558-47.2.433 .

Vidanović, Dejan, Šekler, Milanko, Ašanin, Ružica, Milić, Nenad, Nišavić, Jakov, Petrović, Tamaš, Savić, Vladimir, "Characterization of velogenic newcastle disease viruses isolated from dead wild birds in Serbia during 2007" in Journal of Wildlife Diseases, 47, no. 2 (2011):433-441,
https://doi.org/10.7589/0090-3558-47.2.433 . .

TY  - JOUR
AU  - Gavrović, Miloš
AU  - Ašanin, Ružica
AU  - Mišić, Dušan
AU  - Jezdimirović, Milanka
AU  - Žutić, Milenko
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/823
AB  - Resistance to antibiotics is not a modern phenomenon. On the contrary, penicillin resistance in some bacterial strains developed quickly after its introduction into daily practice. At the same time some bacterial strains developed resistance to almost all known antibiotics, vancomycin included. Vancomycin was for a long time the only efficient antibiotic against staphylococcal infections. It is of special concern the fact that antibiotics are in everyday exploitation in agriculture and veterinary clinical practice which use them not only as a mean of therapeutic treatment, but as an additive in animal feedstuffs in order to promote growth and prevent bacterial infections. The same antibiotics are used in human medicine, which is a persistent problem. In such a way it is possible to develop resistance which can be transferred to human pathogenic bacteria via mobile genetic elements. The incidence of resistant bacterial strains increases year after year not only on a local level, but on a global scale, as well. Monitoring of the use of antibiotics and chemotherapeutics in the Republic of Serbia is not established as such, our intention was to study a number of bacteria isolated from cattle, pigs, poultry, dogs and cats. At this time we are presenting the results for pathogenic strains of E. coli in order to determine the use of antibiotics and chemotherapeutics of the old and new generations in domestic animals. E. coli sensitivity was investigated with the disc diffusion test for: ampicillin, amoxicillin with clavulanic acid, tetracycline, chloramphenicol, gentamicin, and ceftriaxon, sulphamethoxasole with trimethoprim, ciprofloxacin and florfenicol. E. coli strains resistant to three or more antibiotics were tested by means of agar dilution method for ciprofloxacin, tetracycline, chloramphenicol, gentamicin and amoxicillin with clavulanic acid by determination of the minimal inhibitory concentration (MIC). The tested E. coli strains resulted resistant to all antibiotics and chemotherapeutics with the exception of ceftriaxon and florfenicol. The highest resistance incidence (87.5%) was to tetracycline in E. coli strains isolated from pigs, 60% for E. coli strains isolated from cattle, 56% isolated from poultry and 20% originating from dogs. E. coli strains isolated from cats were sensitive to tetracycline. The highest incidence of ampicillin resistance was determined for E. coli strains originated from poultry (78%).
AB  - Rezistencija na antibiotike nije skorašnji fenomen, naprotiv, rezistencija na penicilin kod nekih sojeva bakterija javila se vrlo brzo posle njegove primene u praksi. Takođe, neki sojevi bakterija postali su rezistentni na praktično sve antibiotike u kliničkoj upotrebi, pa čak i na vankomicin koji je duže vreme bio jedini efikasan antibiotik u lečenju stafilokoknih infekcija. Poseban razlog za zabrinutost predstavlja upotreba antibiotika u poljoprivredi i veterinarskoj kliničkoj praksi jer se osim primene u lečenju životinja, iako su zabranjeni, koriste kao promoteri rasta i u prevenciji bakterijskih infekcija, a isti antibiotici (ili sa istim načinom delovanja na bakterije) primenjuju se i u humanoj medicini. Na taj način je moguće da se neodgovornom upotrebom antibiotika razvije rezistencija koja može znatno brže da se prenese na patogene bakterije ljudi preko mobilnih genetičkih elemenata. Pojava rezistentnih sojeva bakterija se povećava iz godine u godinu, kako na lokalnom, tako i na globalnom nivou. Kako monitoring korišćenja antibiotika i hemioterapeutika u veterinarskoj medicini u Republici Srbiji nije uspostavljen, a problem rezistencije bakterija na antibakterijske lekove je prisutan, ovim ispitivanjem želeli smo da obuhvatimo više vrsta bakterija izolovanih od goveda, svinja, živine, pasa i mačaka. Rezultate dobijene u ovom ispitivanju iznosimo samo za patogene sojeve E. coli, kako bi na indirektan način ustanovili primenu antibiotika i hemioterapeutika starije i novije generacije kod pomenutih životinja. Osetljivost navedenih sojeva E. coli ispitivana je na: ampicilin, amoksicilin sa klavulanskom kiselinom, tetraciklin, hloramfenikol, gentamicin, ceftriakson, sulfometoksazol sa trimetoprimom, ciprofloksacin i florfenikol, primenom disk difuzione metode. Kod sojeva E. coli kod kojih je primenom disk-difuzione metode ustanovljena rezistencija na tri i više antibiotika, odnosno multi rezistencija dalje ispitivanje je vršeno i dilucionom metodom u agaru na: ciprofloksacin, tetraciklin, hloramfenikol, gentamicin i amoksicilin sa klavulanskom kiselinom radi utvrđivanja minimalne inhibitorne koncentracije (MIC). Kod ispitivanih sojeva E.coli ustanovljena je rezistencija na sve ispitivane antibiotike i hemioterapeutike, izuzev na ceftriakson i florfenikol. Najveći procenat rezistencije od 87,5% ustanovljen je na tetraciklin kod sojeva E.coli izolovanih od svinja, a zatim 60% kod sojeva izolovanih od goveda, od 56% kod sojeva izolovanih od živine i od 20% kod sojeva poreklom od pasa, dok su sojevi E. coli poreklom od mačaka bili osetljivi na tetraciklin. Najviši procenat rezistencije na ampicilin od 78% ustanovljen je kod sojeva E. coli izolovanih od živine.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Investigation of the sensitivity of E. coli strains isolated from domestic animals to antibiotics and hemiotherapeutics in vitro
T1  - Ispitivanje in vitro osetljivosti na antibiotike i hemioterapeutike sojeva E. coli izolovanih od životinja
VL  - 61
IS  - 1
SP  - 21
EP  - 31
DO  - 10.2298/AVB1101021G
ER  - 

@article{
author = "Gavrović, Miloš and Ašanin, Ružica and Mišić, Dušan and Jezdimirović, Milanka and Žutić, Milenko",
year = "2011",
abstract = "Resistance to antibiotics is not a modern phenomenon. On the contrary, penicillin resistance in some bacterial strains developed quickly after its introduction into daily practice. At the same time some bacterial strains developed resistance to almost all known antibiotics, vancomycin included. Vancomycin was for a long time the only efficient antibiotic against staphylococcal infections. It is of special concern the fact that antibiotics are in everyday exploitation in agriculture and veterinary clinical practice which use them not only as a mean of therapeutic treatment, but as an additive in animal feedstuffs in order to promote growth and prevent bacterial infections. The same antibiotics are used in human medicine, which is a persistent problem. In such a way it is possible to develop resistance which can be transferred to human pathogenic bacteria via mobile genetic elements. The incidence of resistant bacterial strains increases year after year not only on a local level, but on a global scale, as well. Monitoring of the use of antibiotics and chemotherapeutics in the Republic of Serbia is not established as such, our intention was to study a number of bacteria isolated from cattle, pigs, poultry, dogs and cats. At this time we are presenting the results for pathogenic strains of E. coli in order to determine the use of antibiotics and chemotherapeutics of the old and new generations in domestic animals. E. coli sensitivity was investigated with the disc diffusion test for: ampicillin, amoxicillin with clavulanic acid, tetracycline, chloramphenicol, gentamicin, and ceftriaxon, sulphamethoxasole with trimethoprim, ciprofloxacin and florfenicol. E. coli strains resistant to three or more antibiotics were tested by means of agar dilution method for ciprofloxacin, tetracycline, chloramphenicol, gentamicin and amoxicillin with clavulanic acid by determination of the minimal inhibitory concentration (MIC). The tested E. coli strains resulted resistant to all antibiotics and chemotherapeutics with the exception of ceftriaxon and florfenicol. The highest resistance incidence (87.5%) was to tetracycline in E. coli strains isolated from pigs, 60% for E. coli strains isolated from cattle, 56% isolated from poultry and 20% originating from dogs. E. coli strains isolated from cats were sensitive to tetracycline. The highest incidence of ampicillin resistance was determined for E. coli strains originated from poultry (78%)., Rezistencija na antibiotike nije skorašnji fenomen, naprotiv, rezistencija na penicilin kod nekih sojeva bakterija javila se vrlo brzo posle njegove primene u praksi. Takođe, neki sojevi bakterija postali su rezistentni na praktično sve antibiotike u kliničkoj upotrebi, pa čak i na vankomicin koji je duže vreme bio jedini efikasan antibiotik u lečenju stafilokoknih infekcija. Poseban razlog za zabrinutost predstavlja upotreba antibiotika u poljoprivredi i veterinarskoj kliničkoj praksi jer se osim primene u lečenju životinja, iako su zabranjeni, koriste kao promoteri rasta i u prevenciji bakterijskih infekcija, a isti antibiotici (ili sa istim načinom delovanja na bakterije) primenjuju se i u humanoj medicini. Na taj način je moguće da se neodgovornom upotrebom antibiotika razvije rezistencija koja može znatno brže da se prenese na patogene bakterije ljudi preko mobilnih genetičkih elemenata. Pojava rezistentnih sojeva bakterija se povećava iz godine u godinu, kako na lokalnom, tako i na globalnom nivou. Kako monitoring korišćenja antibiotika i hemioterapeutika u veterinarskoj medicini u Republici Srbiji nije uspostavljen, a problem rezistencije bakterija na antibakterijske lekove je prisutan, ovim ispitivanjem želeli smo da obuhvatimo više vrsta bakterija izolovanih od goveda, svinja, živine, pasa i mačaka. Rezultate dobijene u ovom ispitivanju iznosimo samo za patogene sojeve E. coli, kako bi na indirektan način ustanovili primenu antibiotika i hemioterapeutika starije i novije generacije kod pomenutih životinja. Osetljivost navedenih sojeva E. coli ispitivana je na: ampicilin, amoksicilin sa klavulanskom kiselinom, tetraciklin, hloramfenikol, gentamicin, ceftriakson, sulfometoksazol sa trimetoprimom, ciprofloksacin i florfenikol, primenom disk difuzione metode. Kod sojeva E. coli kod kojih je primenom disk-difuzione metode ustanovljena rezistencija na tri i više antibiotika, odnosno multi rezistencija dalje ispitivanje je vršeno i dilucionom metodom u agaru na: ciprofloksacin, tetraciklin, hloramfenikol, gentamicin i amoksicilin sa klavulanskom kiselinom radi utvrđivanja minimalne inhibitorne koncentracije (MIC). Kod ispitivanih sojeva E.coli ustanovljena je rezistencija na sve ispitivane antibiotike i hemioterapeutike, izuzev na ceftriakson i florfenikol. Najveći procenat rezistencije od 87,5% ustanovljen je na tetraciklin kod sojeva E.coli izolovanih od svinja, a zatim 60% kod sojeva izolovanih od goveda, od 56% kod sojeva izolovanih od živine i od 20% kod sojeva poreklom od pasa, dok su sojevi E. coli poreklom od mačaka bili osetljivi na tetraciklin. Najviši procenat rezistencije na ampicilin od 78% ustanovljen je kod sojeva E. coli izolovanih od živine.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Investigation of the sensitivity of E. coli strains isolated from domestic animals to antibiotics and hemiotherapeutics in vitro, Ispitivanje in vitro osetljivosti na antibiotike i hemioterapeutike sojeva E. coli izolovanih od životinja",
volume = "61",
number = "1",
pages = "21-31",
doi = "10.2298/AVB1101021G"
}

Gavrović, M., Ašanin, R., Mišić, D., Jezdimirović, M.,& Žutić, M.. (2011). Investigation of the sensitivity of E. coli strains isolated from domestic animals to antibiotics and hemiotherapeutics in vitro. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(1), 21-31.
https://doi.org/10.2298/AVB1101021G

Gavrović M, Ašanin R, Mišić D, Jezdimirović M, Žutić M. Investigation of the sensitivity of E. coli strains isolated from domestic animals to antibiotics and hemiotherapeutics in vitro. in Acta Veterinaria-Beograd. 2011;61(1):21-31.
doi:10.2298/AVB1101021G .

Gavrović, Miloš, Ašanin, Ružica, Mišić, Dušan, Jezdimirović, Milanka, Žutić, Milenko, "Investigation of the sensitivity of E. coli strains isolated from domestic animals to antibiotics and hemiotherapeutics in vitro" in Acta Veterinaria-Beograd, 61, no. 1 (2011):21-31,
https://doi.org/10.2298/AVB1101021G . .

TY  - JOUR
AU  - Žutić, Jadranka
AU  - Ašanin, Jelena
AU  - Mišić, Dušan
AU  - Jakić-Dimić, Dobrila
AU  - Milić, Nenad
AU  - Ašanin, Ružica
AU  - Stojanović, Dragica
AU  - Žutić, Milenko
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/672
AB  - Among different pathogens, enterotoxic E. coli (ETEC) has been for many years an important etiological agent in the occurrence of digestive system disease of newborn animals. In counties with developed pig breeding (farming), including our country, diarrhea in the neonatal period, caused by ETEC strains is one of the most present and economically most significant diseases. The aim of this investigation wais to determine the prevalence of ETEC strains in piglets (weaning pigs), originated from 5 (five) pig farms in the Republic of Serbia, as well as their serological typization based on characteristics of somatic O antigens, presence of fimbrial antigensadhesins and hemolytic activity. The material for this investigation was targeted and sampled from piglets that have shown clinical signs of neonatal diarrhea or pathoanatomical changes characteristic for enteritis caused by ETEC strains. The total number of isolated ETEC strains were 148, of which 91 (61.48 %) were determined on the basis of somatic O antigen characteristics. The largest number of strains, 42 (46.15 %) belonged to serotype O149. Serological types O8 and O147 were represented, each with 15 strains (16.48 %). In 13 (14.28 %) strains the somatic antigen which belonged to serotype O138 was determined and in 6 (6.59 %) strains the antigen belonged to serotype O157. No strain agglutinated with hyperimmune O139 serotype serum. The presence of fimbrial adhesins was determined in 47 (51.64%) strains and of that number the F4 type of fimbrial adhesins was detected in 38 (80.85 %) strains. The presence of F5 adhesins was determined in 4, and F6 in 3. In 2 strains, the paralell presence of two adhesin types, F4 and F6 was detected. The greatest number of strains 30 (71.42 %) with adhesin F4 belonged to O149 serotype, a considerably smaller number, 4 (26.66%) to serotype O8, 2 strains to serotype O157 and to each serotype O147 and O138 1 strain. The fimbrial adhesin of F5 type was detected in 3 strains which belonged to serotype O8 and in 1 strain of serotype O149. All 3 strains with F6 adhesin, belonged to serotype O8. From 2 strains which had, at the same time, adhesins F4 and F6 one belonged to serotype O8 and the other to serotype O138. Hemolytic activity was present in 42 (46.15 %) strains, of which 34 strains belonged to O149 serotype, 6 strains to O157 serotype and 2 strains to O147 serotype.
AB  - Među različitim patogenima, enterotoksična E.coli (ETEC) je već dugo značajan etiološki agens u nastanku oboljenja digestivnog sistema novorođenih životinja. U zemljama sa razvijenom svinjarskom proizvodnjom, uključujući i našu zemlju, dijareja u neonatalnom periodu, uzrokovana sojevima ETEC je jedna od najprisutnijih i ekonomski najznačajnijih bolesti. Cilj istraživanja je bio da se utvrdi prevalencija sojeva ETEC kod prasadi, poreklom sa 5 farmi svinja u Srbiji, kao i njihova serološka tipizacija na osnovu karakteristika somatskog O antigena, prisustva fimbrijalnih antigena - adhezina i hemolitičke aktivnosti. Materijal za istraživanja ciljano je uzorkovan od prasadi koja su pokazivala kliničke znake neonatalne dijareje ili patoanatomske promene karakteristične za enteritise izazvane sojevima ETEC. Ukupno je izolovano 148 sojeva ETEC od čega je 91 soj (61,48%) bio tipiziran na osnovu karakteristika somatskog O antigena. Najveći broj sojeva, 42 (46,15%) pripadao je serotipu O149. Serološke grupe O8 i O147 su bile zastupljene sa po 15 (16,48%) sojeva. Kod 13 (14,28 %) sojeva, utvrđen je somatski antigen serogrupe O138, a kod 6 (6,59%) sojeva antigen serogrupe O157. Nijedan soj nije aglutinirao sa hiperimunim serumom serogrupe O139. Prisustvo fimbrijalnih adhezina ustanovljeno je kod 47 (51,64 %) sojeva, a od tog broja F4 tip fimbrijalnih adhezina detektovan je kod 38 (80,85 %) sojeva. Prisustvo F5 adhezina ustanovljeno je kod 4, F6 kod 3, a kod 2 soja detektovano je istovremeno prisustvo dva tipa adhezina, F4 i F6. Najveći broj sojeva, 30 (71,42 %) sa F4 adhezinom pripadao je serogrupi O149, znatno manji broj, 4 (26,66 %) serogrupi O8, 2 soja serogrupi O157 i po 1 soj serogrupama O147 i O138. Fimbrijalni adhezin tipa F5 otkriven je kod 3 soja serogrupe O8 i kod 1 soja iz serogrupe O149. Sva 3 soja sa F6 adhezinom pripadala su serogrupi O8. Od 2 soja sa istovremenim prisustvom adhezina F4 i F6, jedan je pripadao serogrupi O8, a drugi serogrupi O138. Hemolitička aktivnost je bila prisutna kod 42 (46,15%) soja, od kojih su 34 soja pripadala serotipu O149, 6 sojeva serotipu O157 i 2 soja serotipu O147.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens
T1  - Izolacija sojeva ETEC od prasadi sa dijarejom u neonatalnom periodu i njihova tipizacija na osnovu somatskog i fimbrijalnih antigena
VL  - 60
IS  - 5-6
SP  - 497
EP  - 506
DO  - 10.2298/AVB1006497Z
ER  - 

@article{
author = "Žutić, Jadranka and Ašanin, Jelena and Mišić, Dušan and Jakić-Dimić, Dobrila and Milić, Nenad and Ašanin, Ružica and Stojanović, Dragica and Žutić, Milenko",
year = "2010",
abstract = "Among different pathogens, enterotoxic E. coli (ETEC) has been for many years an important etiological agent in the occurrence of digestive system disease of newborn animals. In counties with developed pig breeding (farming), including our country, diarrhea in the neonatal period, caused by ETEC strains is one of the most present and economically most significant diseases. The aim of this investigation wais to determine the prevalence of ETEC strains in piglets (weaning pigs), originated from 5 (five) pig farms in the Republic of Serbia, as well as their serological typization based on characteristics of somatic O antigens, presence of fimbrial antigensadhesins and hemolytic activity. The material for this investigation was targeted and sampled from piglets that have shown clinical signs of neonatal diarrhea or pathoanatomical changes characteristic for enteritis caused by ETEC strains. The total number of isolated ETEC strains were 148, of which 91 (61.48 %) were determined on the basis of somatic O antigen characteristics. The largest number of strains, 42 (46.15 %) belonged to serotype O149. Serological types O8 and O147 were represented, each with 15 strains (16.48 %). In 13 (14.28 %) strains the somatic antigen which belonged to serotype O138 was determined and in 6 (6.59 %) strains the antigen belonged to serotype O157. No strain agglutinated with hyperimmune O139 serotype serum. The presence of fimbrial adhesins was determined in 47 (51.64%) strains and of that number the F4 type of fimbrial adhesins was detected in 38 (80.85 %) strains. The presence of F5 adhesins was determined in 4, and F6 in 3. In 2 strains, the paralell presence of two adhesin types, F4 and F6 was detected. The greatest number of strains 30 (71.42 %) with adhesin F4 belonged to O149 serotype, a considerably smaller number, 4 (26.66%) to serotype O8, 2 strains to serotype O157 and to each serotype O147 and O138 1 strain. The fimbrial adhesin of F5 type was detected in 3 strains which belonged to serotype O8 and in 1 strain of serotype O149. All 3 strains with F6 adhesin, belonged to serotype O8. From 2 strains which had, at the same time, adhesins F4 and F6 one belonged to serotype O8 and the other to serotype O138. Hemolytic activity was present in 42 (46.15 %) strains, of which 34 strains belonged to O149 serotype, 6 strains to O157 serotype and 2 strains to O147 serotype., Među različitim patogenima, enterotoksična E.coli (ETEC) je već dugo značajan etiološki agens u nastanku oboljenja digestivnog sistema novorođenih životinja. U zemljama sa razvijenom svinjarskom proizvodnjom, uključujući i našu zemlju, dijareja u neonatalnom periodu, uzrokovana sojevima ETEC je jedna od najprisutnijih i ekonomski najznačajnijih bolesti. Cilj istraživanja je bio da se utvrdi prevalencija sojeva ETEC kod prasadi, poreklom sa 5 farmi svinja u Srbiji, kao i njihova serološka tipizacija na osnovu karakteristika somatskog O antigena, prisustva fimbrijalnih antigena - adhezina i hemolitičke aktivnosti. Materijal za istraživanja ciljano je uzorkovan od prasadi koja su pokazivala kliničke znake neonatalne dijareje ili patoanatomske promene karakteristične za enteritise izazvane sojevima ETEC. Ukupno je izolovano 148 sojeva ETEC od čega je 91 soj (61,48%) bio tipiziran na osnovu karakteristika somatskog O antigena. Najveći broj sojeva, 42 (46,15%) pripadao je serotipu O149. Serološke grupe O8 i O147 su bile zastupljene sa po 15 (16,48%) sojeva. Kod 13 (14,28 %) sojeva, utvrđen je somatski antigen serogrupe O138, a kod 6 (6,59%) sojeva antigen serogrupe O157. Nijedan soj nije aglutinirao sa hiperimunim serumom serogrupe O139. Prisustvo fimbrijalnih adhezina ustanovljeno je kod 47 (51,64 %) sojeva, a od tog broja F4 tip fimbrijalnih adhezina detektovan je kod 38 (80,85 %) sojeva. Prisustvo F5 adhezina ustanovljeno je kod 4, F6 kod 3, a kod 2 soja detektovano je istovremeno prisustvo dva tipa adhezina, F4 i F6. Najveći broj sojeva, 30 (71,42 %) sa F4 adhezinom pripadao je serogrupi O149, znatno manji broj, 4 (26,66 %) serogrupi O8, 2 soja serogrupi O157 i po 1 soj serogrupama O147 i O138. Fimbrijalni adhezin tipa F5 otkriven je kod 3 soja serogrupe O8 i kod 1 soja iz serogrupe O149. Sva 3 soja sa F6 adhezinom pripadala su serogrupi O8. Od 2 soja sa istovremenim prisustvom adhezina F4 i F6, jedan je pripadao serogrupi O8, a drugi serogrupi O138. Hemolitička aktivnost je bila prisutna kod 42 (46,15%) soja, od kojih su 34 soja pripadala serotipu O149, 6 sojeva serotipu O157 i 2 soja serotipu O147.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens, Izolacija sojeva ETEC od prasadi sa dijarejom u neonatalnom periodu i njihova tipizacija na osnovu somatskog i fimbrijalnih antigena",
volume = "60",
number = "5-6",
pages = "497-506",
doi = "10.2298/AVB1006497Z"
}

Žutić, J., Ašanin, J., Mišić, D., Jakić-Dimić, D., Milić, N., Ašanin, R., Stojanović, D.,& Žutić, M.. (2010). Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(5-6), 497-506.
https://doi.org/10.2298/AVB1006497Z

Žutić J, Ašanin J, Mišić D, Jakić-Dimić D, Milić N, Ašanin R, Stojanović D, Žutić M. Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens. in Acta Veterinaria-Beograd. 2010;60(5-6):497-506.
doi:10.2298/AVB1006497Z .

Žutić, Jadranka, Ašanin, Jelena, Mišić, Dušan, Jakić-Dimić, Dobrila, Milić, Nenad, Ašanin, Ružica, Stojanović, Dragica, Žutić, Milenko, "Isolation of ETEC strains from piglets with diarrhea in the neonatal period and their typization based on somatic and fimbrial antigens" in Acta Veterinaria-Beograd, 60, no. 5-6 (2010):497-506,
https://doi.org/10.2298/AVB1006497Z . .

TY  - JOUR
AU  - Milić, Nenad
AU  - Nišavić, Jakov
AU  - Ašanin, Ružica
AU  - Knežević, Aleksandra
AU  - Ašanin, Jelena
AU  - Vidanović, Dejan
AU  - Šekler, Milanko
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/746
AB  - Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard methods of virological diagnostics. The appearance of a cytopathogenic effect (CPE) was not established in inoculated cell lines 24h, 48h and 72h following inoculation, or after two successive passages of the examined material sample through these cell lines. The application of polymerize chain reaction (PCR) using a primer for glucoprotein B and thymidine - kinasis, established the presence of bovine herpes virus 1 nucleic acid in one sample of a bovine nasal smear, while the presence of this virus was established in three samples in an examination of the nasal smear samples using the Real-Time PCR method.
AB  - Ukupno je ispitivano 65 uzoraka nosnih briseva prikupljenih od teladi i junadi sa kliničkim simptomima respiratorne infekcije na prisustvo goveđeg herpesvirusa 1 uporednom primenom molekularnih i standardnih metoda virusološke dijagnostike. Kod inokulisanih ćelijskih linija nije ustanovljena pojava citopatogenog efekta (CPE -) posle 24h, 48h i 72h od inokulacije ni posle dve uzastopne pasaže uzoraka ispitivanog materijala kroz navedene ćelijske linije. Primenom lančane reakcije polimeraze (PCR) uz korišćenje prajmera za glikoprotein B i timidin-kinazu, utvrđeno je prisustvo nukleinske kiseline goveđeg herpesvirusa 1 u jednom uzorku nosnog brisa, dok je ispitivanjem navedenih uzoraka nosnih briseva goveda metodom Real-Time PCR prisustvo pomenutog virusa ustanovljeno kod tri uzorka.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1
T1  - Primena lančane reakcije polimeraze (PCR) i metode Real-Time PCR u brzoj identifikaciji goveđeg herpesvirusa 1
VL  - 64
IS  - 3-4
SP  - 159
EP  - 167
DO  - 10.2298/VETGL1004159M
ER  - 

@article{
author = "Milić, Nenad and Nišavić, Jakov and Ašanin, Ružica and Knežević, Aleksandra and Ašanin, Jelena and Vidanović, Dejan and Šekler, Milanko",
year = "2010",
abstract = "Examinations were performed on 65 samples of nasal smeas taken from calves and young cows with clinical symptoms of respiratory infection to determine the presence of the bovine herpes virus 1 using parallel implementation of molecular and standard methods of virological diagnostics. The appearance of a cytopathogenic effect (CPE) was not established in inoculated cell lines 24h, 48h and 72h following inoculation, or after two successive passages of the examined material sample through these cell lines. The application of polymerize chain reaction (PCR) using a primer for glucoprotein B and thymidine - kinasis, established the presence of bovine herpes virus 1 nucleic acid in one sample of a bovine nasal smear, while the presence of this virus was established in three samples in an examination of the nasal smear samples using the Real-Time PCR method., Ukupno je ispitivano 65 uzoraka nosnih briseva prikupljenih od teladi i junadi sa kliničkim simptomima respiratorne infekcije na prisustvo goveđeg herpesvirusa 1 uporednom primenom molekularnih i standardnih metoda virusološke dijagnostike. Kod inokulisanih ćelijskih linija nije ustanovljena pojava citopatogenog efekta (CPE -) posle 24h, 48h i 72h od inokulacije ni posle dve uzastopne pasaže uzoraka ispitivanog materijala kroz navedene ćelijske linije. Primenom lančane reakcije polimeraze (PCR) uz korišćenje prajmera za glikoprotein B i timidin-kinazu, utvrđeno je prisustvo nukleinske kiseline goveđeg herpesvirusa 1 u jednom uzorku nosnog brisa, dok je ispitivanjem navedenih uzoraka nosnih briseva goveda metodom Real-Time PCR prisustvo pomenutog virusa ustanovljeno kod tri uzorka.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1, Primena lančane reakcije polimeraze (PCR) i metode Real-Time PCR u brzoj identifikaciji goveđeg herpesvirusa 1",
volume = "64",
number = "3-4",
pages = "159-167",
doi = "10.2298/VETGL1004159M"
}

Milić, N., Nišavić, J., Ašanin, R., Knežević, A., Ašanin, J., Vidanović, D.,& Šekler, M.. (2010). Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 64(3-4), 159-167.
https://doi.org/10.2298/VETGL1004159M

Milić N, Nišavić J, Ašanin R, Knežević A, Ašanin J, Vidanović D, Šekler M. Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1. in Veterinarski Glasnik. 2010;64(3-4):159-167.
doi:10.2298/VETGL1004159M .

Milić, Nenad, Nišavić, Jakov, Ašanin, Ružica, Knežević, Aleksandra, Ašanin, Jelena, Vidanović, Dejan, Šekler, Milanko, "Implementation of polymerase chain reaction (PCR) and Real-Time PCR in quick identification of bovine herpesvirus 1" in Veterinarski Glasnik, 64, no. 3-4 (2010):159-167,
https://doi.org/10.2298/VETGL1004159M . .

TY  - JOUR
AU  - Šekler, Milanko
AU  - Ašanin, Ružica
AU  - Krnjaić, Dejan
AU  - Palić, T.
AU  - Milić, Nenad
AU  - Jovanović, Tanja
AU  - Kovačević, Dragana
AU  - Plavšić, B.
AU  - Stojanović, Dragica
AU  - Vidanović, Dejan
AU  - Ašanin, N.
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/652
AB  - Infections caused by the avian influenza virus have been known for a long time and they are present, to a smaller or greater extent, in both extensive and intensive poultry production in many parts of the world. Epidemiological investigations have established a definite significance of the population of wild birds in maintaining and spreading this infection. Avian influenza is a zoonosis, and the virus has a great potential for causing mortality in humans, in particular its subtypes H5 and H7, which is why it has lately been provoking much attention among scientists and experts, as well as the general public. The objective of the work was to catch a certain number of wild birds in several locations in the Republic of Serbia, to identify them, and to collect samples of their blood serum for the determination of specific antibodies against the avian influenza virus. Birds were caught in ten locations in a manner that was safe for the birds themselves, as well as for the staff that did the catching. The birds were caught in especially produced nets, and in some cases in special traps. The caught wild birds were identified using the methods described in reference literature. All the names of the wild birds were coordinated with the valid Serbian nomenclature of European birds, prepared by prominent ornithologist and bird lover Milan Ružić. Following catching and identification, blood samples were taken from the birds from the wing vein (in bigger birds) or from the leg vein (in smaller birds). In taking blood samples from wild birds, all the principles of asepsis and antisepsis were followed in order to prevent any possibility of infection. After that, the birds were returned to their natural habitat, to the same locations in which they were caught. Serums were isolated from the taken blood samples and they were stored at -20ºC until the final examinations. A total of 46 species of wild birds were identified among a total of 259 birds from which 259 samples of blood serum were isolated. The following were used for the detection of the presence of specific antibodies against the avian influenza virus in blood serum samples of wild birds: agar gel precipitation (AGP), the hemagglutination inhibition test (HI) for subtypes H5 and H7, the cELISA test with antigen for the A type avian infleunza virus, and the cELISA test with antigen for subtype H5 of the avian influenza virus. Due to the fact that about 360 different species of wild birds live in the Republic of Serbia, the number of 46 identified species covered by these investigations account for 12.77% of the total number of bird species present in Serbia, which is considered a good sample. Specific antibodies against the A type avian influenza virus were established in serum samples of only 9 of the 259 birds covered by examinations using the cELISA test. Of the 46 identified wild bird species, 6 belonged to birds that live exclusively in water habitats and are considered a reservoir of the avian influenza virus (white stork, mallard, mute swan, common pochard, common goldeneye, and Eurasian coot). Among the listed species, particular attention was drawn to 4 species of wild birds of the order Anseriformes and the family Anatidae (mallard, mute swan, common pochard, common goldeneye) of which there were 30 birds among the total of 259 examined. In the 30 blood serum samples of the listed bird species, specific antibodies against the A type avian influenza virus were established in 9 (30%) serum samples using cELISA. Specific antibodies against the avian influenza virus subtype H5 were established in 3 serum samples of mute swans (one serum sample originated from a mute swan which was tagged in Poland) and in one blood serum sample of a common pochard, or a total of 4 (13.33%) serum samples, using the hemagglutination inhibition test. Specific antibodies against the avian inluenza virus subtype H7 were established in 3 (10%) blood serum samples, in two serum samples from mallards and one sample from a mute swan, using the hemagglutination inhibition test. Specific antibodies against the avian influenza virus type A were not established in any examined bird species using the AGP test. In the population of wild bird species in the Republic of Serbia covered by these investigations, specific antibodies against the avian influenza virus were established only in serum samples of birds of the family Anatidae. Specific antibodies against the avian influenza virus type A established in 3 (6.52%) species of wild birds, and against subtypes H5 and H7 in 2 (4.34%) of the total of 46 examined species. The sensitivity of the cELISA test for the avian influenza virus subtype H5 and the hemagglutination inhibition test for subtype H5 amounted to 100%.
AB  - Infekcije izazvane virusom avijarne influence, su već odavno poznate i prisutne u manjem ili većem obimu, kako u ekstenzivnoj, tako i u intenzivnoj živinarskoj proizvodnji, u mnogim delovima sveta. Epidemiološkim ispitivanjima je utvrđen nesumnjiv značaj populacije divljih ptica u očuvanju i širenju ove infekcije. Avijarna influenca je zoonoza, a virus ima veliki potencijal da izazove visoku smrtnost kod ljudi, posebno njegovi podtipovi H5 i H7, tako da u novije vreme izaziva veliku pažnju, kako naučne i stručne, tako i najšire javnosti. Cilj ovog rada je bio da se na nekoliko lokacija u Republici Srbiji uhvati određeni broj divljih ptica, izvrši njihova identifikacija i prikupe uzorci krvnog seruma radi otkrivanja specifičnih antitela protiv virusa avijarne influence. Hvatanje ptica vršeno je na deset lokacija na bezbedan način, kako za same ptice, tako i za osoblje koje ih je hvatalo. Hvatanje ptica obavljano je posebnim za te svrhe proizvedenim mrežama, a u nekim slučajevima i posebnim zamkama (klopkama). Za identifikaciju uhvaćenih divljih ptica korišćene su metode koje su opisane u stručnoj literaturi. Svi nazivi divljih ptica usklađeni su sa važećom srpskom nomenklaturom ptica Evrope. Nakon hvatanja i identifikacije, pticama je uzimana krv iz krilne vene (kod većih ptica) ili iz nožne vene (kod malih ptica). Prilikom uzimanja krvi od divljih ptica poštovani su svi principi asepse i antisepse, kako bi se sprečila svaka mogućnost infekcije. Nakon toga, ptice su vraćane u prirodu, na iste lokacije na kojima su i uhvaćene. Od uzetih uzoraka krvi izdvojeni su serumi koji su ostavljani na - 20ºC i čuvani do konačnog ispitivanja. Identifikovano je 46 vrsta divljih ptica sa ukupno 259 jedinki od kojih je izdvojeno 259 uzoraka krvnog seruma. Za otkrivanje prisustva specifičnih antitela protiv virusa avijarne influence u uzorcima krvnog seruma divljih ptica korišćeni su agar gel precipitacija (AGP), test inhibicije hemaglutinacije (IH) za podtipove H5 i H7, cELISA test sa antigenom A tipa virusa avijarne influence i cELISA sa antigenom podtipa H5 virusa avijarne influence. S obzirom na činjenicu da na teritoriji Republike Srbije živi oko 360 različitih vrsta divljih ptica, broj od 46 identifikovanih vrsta obuhvaćenih ispitivanjem, činio je 12,77% od ukupnog broja prisutnih vrsta ptica u Srbiji, što se smatra dobrim uzorkom. Specifična antitela protiv A tipa virusa avijarne influence ustanovljena su u uzorcima seruma samo 9 od 259 jedinki koje su bile obuhvaćene ispitivanjem primenom cELISA testa. U identifikovanih 46 vrsta divljih ptica 6 je pripadalo pticama koje žive isključivo u vodenim staništima i smatraju se rezervoarom virusa avijarne influence (bela roda, patka gluvara, labud grbac, riđoglava patka, patka dupljašica i liska). Od navedenih vrsta posebnu pažnju privukle su 4 vrste divljih ptica iz reda Anseriformes i familije Anatidae (patka gluvara, labud grbac, riđoglava patka, patka dupljašica) kojima je od ukupno 259 ptica pripadalo 30 jedinki. U 30 uzoraka krvnog seruma navedenih vrsta ptica, specifična antitela protiv A tipa virusa avijarne influence utvrđena su u 9 (30%) uzoraka seruma, primenom cELISA. Specifična antitela protiv podtipa H5 virusa avijarne influence su ustanovljena u 3 uzorka seruma labudova grbaca (jedan uzorak seruma je poticao od labuda grbca koji je prstenovan u Poljskoj) i u jednom uzorku krvnog seruma riđoglave patke, ili ukupno u 4 (13,33%) uzorka seruma, primenom testa inhibicije hemaglutinacije. Specifična antitela protiv podtipa H7 virusa avijarne influence utvrđena su u 3 (10%) uzorka krvnog seruma i to u dva seruma pataka gluvara i u jednom serumu labuda grbca, primenom inhibicije hemaglutinacije. Specifična antitela protiv A tipa virusa avijarne influence nisu ustanovljena ni kod jedne ispitivane vrste ptice, primenom AGP testa. U populaciji divljih vrta ptica u Republici Srbiji obuhvaćenih ovim ispitivanjem, specifična antitela protiv virusa avijarne influence ustanovljena su samo u uzorcima seruma ptica iz familije Anatidae. Specifična antitela protiv A tipa virusa avijarne influence su otkrivena kod 3 (6,52%) vrste divljih ptica, a protiv podtipova H5 i H7 kod 2 (4,34%) od ukupno 46 vrsta koje su ispitivane. Senzitivnost cELISA testa za podtip H5 virusa avijarne influence i testa inhibicije hemaglutinacije za isti podtip iznosila je 100%.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Examination of presence of specific antibodies against avian influenza virus in some species of wild birds
T1  - Ispitivanje prisustva specifičnih antitela protiv virusa avijarne influence kod nekih vrsta divljih ptica
VL  - 59
IS  - 4
SP  - 381
EP  - 403
DO  - 10.2298/AVB0904381S
ER  - 

@article{
author = "Šekler, Milanko and Ašanin, Ružica and Krnjaić, Dejan and Palić, T. and Milić, Nenad and Jovanović, Tanja and Kovačević, Dragana and Plavšić, B. and Stojanović, Dragica and Vidanović, Dejan and Ašanin, N.",
year = "2009",
abstract = "Infections caused by the avian influenza virus have been known for a long time and they are present, to a smaller or greater extent, in both extensive and intensive poultry production in many parts of the world. Epidemiological investigations have established a definite significance of the population of wild birds in maintaining and spreading this infection. Avian influenza is a zoonosis, and the virus has a great potential for causing mortality in humans, in particular its subtypes H5 and H7, which is why it has lately been provoking much attention among scientists and experts, as well as the general public. The objective of the work was to catch a certain number of wild birds in several locations in the Republic of Serbia, to identify them, and to collect samples of their blood serum for the determination of specific antibodies against the avian influenza virus. Birds were caught in ten locations in a manner that was safe for the birds themselves, as well as for the staff that did the catching. The birds were caught in especially produced nets, and in some cases in special traps. The caught wild birds were identified using the methods described in reference literature. All the names of the wild birds were coordinated with the valid Serbian nomenclature of European birds, prepared by prominent ornithologist and bird lover Milan Ružić. Following catching and identification, blood samples were taken from the birds from the wing vein (in bigger birds) or from the leg vein (in smaller birds). In taking blood samples from wild birds, all the principles of asepsis and antisepsis were followed in order to prevent any possibility of infection. After that, the birds were returned to their natural habitat, to the same locations in which they were caught. Serums were isolated from the taken blood samples and they were stored at -20ºC until the final examinations. A total of 46 species of wild birds were identified among a total of 259 birds from which 259 samples of blood serum were isolated. The following were used for the detection of the presence of specific antibodies against the avian influenza virus in blood serum samples of wild birds: agar gel precipitation (AGP), the hemagglutination inhibition test (HI) for subtypes H5 and H7, the cELISA test with antigen for the A type avian infleunza virus, and the cELISA test with antigen for subtype H5 of the avian influenza virus. Due to the fact that about 360 different species of wild birds live in the Republic of Serbia, the number of 46 identified species covered by these investigations account for 12.77% of the total number of bird species present in Serbia, which is considered a good sample. Specific antibodies against the A type avian influenza virus were established in serum samples of only 9 of the 259 birds covered by examinations using the cELISA test. Of the 46 identified wild bird species, 6 belonged to birds that live exclusively in water habitats and are considered a reservoir of the avian influenza virus (white stork, mallard, mute swan, common pochard, common goldeneye, and Eurasian coot). Among the listed species, particular attention was drawn to 4 species of wild birds of the order Anseriformes and the family Anatidae (mallard, mute swan, common pochard, common goldeneye) of which there were 30 birds among the total of 259 examined. In the 30 blood serum samples of the listed bird species, specific antibodies against the A type avian influenza virus were established in 9 (30%) serum samples using cELISA. Specific antibodies against the avian influenza virus subtype H5 were established in 3 serum samples of mute swans (one serum sample originated from a mute swan which was tagged in Poland) and in one blood serum sample of a common pochard, or a total of 4 (13.33%) serum samples, using the hemagglutination inhibition test. Specific antibodies against the avian inluenza virus subtype H7 were established in 3 (10%) blood serum samples, in two serum samples from mallards and one sample from a mute swan, using the hemagglutination inhibition test. Specific antibodies against the avian influenza virus type A were not established in any examined bird species using the AGP test. In the population of wild bird species in the Republic of Serbia covered by these investigations, specific antibodies against the avian influenza virus were established only in serum samples of birds of the family Anatidae. Specific antibodies against the avian influenza virus type A established in 3 (6.52%) species of wild birds, and against subtypes H5 and H7 in 2 (4.34%) of the total of 46 examined species. The sensitivity of the cELISA test for the avian influenza virus subtype H5 and the hemagglutination inhibition test for subtype H5 amounted to 100%., Infekcije izazvane virusom avijarne influence, su već odavno poznate i prisutne u manjem ili većem obimu, kako u ekstenzivnoj, tako i u intenzivnoj živinarskoj proizvodnji, u mnogim delovima sveta. Epidemiološkim ispitivanjima je utvrđen nesumnjiv značaj populacije divljih ptica u očuvanju i širenju ove infekcije. Avijarna influenca je zoonoza, a virus ima veliki potencijal da izazove visoku smrtnost kod ljudi, posebno njegovi podtipovi H5 i H7, tako da u novije vreme izaziva veliku pažnju, kako naučne i stručne, tako i najšire javnosti. Cilj ovog rada je bio da se na nekoliko lokacija u Republici Srbiji uhvati određeni broj divljih ptica, izvrši njihova identifikacija i prikupe uzorci krvnog seruma radi otkrivanja specifičnih antitela protiv virusa avijarne influence. Hvatanje ptica vršeno je na deset lokacija na bezbedan način, kako za same ptice, tako i za osoblje koje ih je hvatalo. Hvatanje ptica obavljano je posebnim za te svrhe proizvedenim mrežama, a u nekim slučajevima i posebnim zamkama (klopkama). Za identifikaciju uhvaćenih divljih ptica korišćene su metode koje su opisane u stručnoj literaturi. Svi nazivi divljih ptica usklađeni su sa važećom srpskom nomenklaturom ptica Evrope. Nakon hvatanja i identifikacije, pticama je uzimana krv iz krilne vene (kod većih ptica) ili iz nožne vene (kod malih ptica). Prilikom uzimanja krvi od divljih ptica poštovani su svi principi asepse i antisepse, kako bi se sprečila svaka mogućnost infekcije. Nakon toga, ptice su vraćane u prirodu, na iste lokacije na kojima su i uhvaćene. Od uzetih uzoraka krvi izdvojeni su serumi koji su ostavljani na - 20ºC i čuvani do konačnog ispitivanja. Identifikovano je 46 vrsta divljih ptica sa ukupno 259 jedinki od kojih je izdvojeno 259 uzoraka krvnog seruma. Za otkrivanje prisustva specifičnih antitela protiv virusa avijarne influence u uzorcima krvnog seruma divljih ptica korišćeni su agar gel precipitacija (AGP), test inhibicije hemaglutinacije (IH) za podtipove H5 i H7, cELISA test sa antigenom A tipa virusa avijarne influence i cELISA sa antigenom podtipa H5 virusa avijarne influence. S obzirom na činjenicu da na teritoriji Republike Srbije živi oko 360 različitih vrsta divljih ptica, broj od 46 identifikovanih vrsta obuhvaćenih ispitivanjem, činio je 12,77% od ukupnog broja prisutnih vrsta ptica u Srbiji, što se smatra dobrim uzorkom. Specifična antitela protiv A tipa virusa avijarne influence ustanovljena su u uzorcima seruma samo 9 od 259 jedinki koje su bile obuhvaćene ispitivanjem primenom cELISA testa. U identifikovanih 46 vrsta divljih ptica 6 je pripadalo pticama koje žive isključivo u vodenim staništima i smatraju se rezervoarom virusa avijarne influence (bela roda, patka gluvara, labud grbac, riđoglava patka, patka dupljašica i liska). Od navedenih vrsta posebnu pažnju privukle su 4 vrste divljih ptica iz reda Anseriformes i familije Anatidae (patka gluvara, labud grbac, riđoglava patka, patka dupljašica) kojima je od ukupno 259 ptica pripadalo 30 jedinki. U 30 uzoraka krvnog seruma navedenih vrsta ptica, specifična antitela protiv A tipa virusa avijarne influence utvrđena su u 9 (30%) uzoraka seruma, primenom cELISA. Specifična antitela protiv podtipa H5 virusa avijarne influence su ustanovljena u 3 uzorka seruma labudova grbaca (jedan uzorak seruma je poticao od labuda grbca koji je prstenovan u Poljskoj) i u jednom uzorku krvnog seruma riđoglave patke, ili ukupno u 4 (13,33%) uzorka seruma, primenom testa inhibicije hemaglutinacije. Specifična antitela protiv podtipa H7 virusa avijarne influence utvrđena su u 3 (10%) uzorka krvnog seruma i to u dva seruma pataka gluvara i u jednom serumu labuda grbca, primenom inhibicije hemaglutinacije. Specifična antitela protiv A tipa virusa avijarne influence nisu ustanovljena ni kod jedne ispitivane vrste ptice, primenom AGP testa. U populaciji divljih vrta ptica u Republici Srbiji obuhvaćenih ovim ispitivanjem, specifična antitela protiv virusa avijarne influence ustanovljena su samo u uzorcima seruma ptica iz familije Anatidae. Specifična antitela protiv A tipa virusa avijarne influence su otkrivena kod 3 (6,52%) vrste divljih ptica, a protiv podtipova H5 i H7 kod 2 (4,34%) od ukupno 46 vrsta koje su ispitivane. Senzitivnost cELISA testa za podtip H5 virusa avijarne influence i testa inhibicije hemaglutinacije za isti podtip iznosila je 100%.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Examination of presence of specific antibodies against avian influenza virus in some species of wild birds, Ispitivanje prisustva specifičnih antitela protiv virusa avijarne influence kod nekih vrsta divljih ptica",
volume = "59",
number = "4",
pages = "381-403",
doi = "10.2298/AVB0904381S"
}

Šekler, M., Ašanin, R., Krnjaić, D., Palić, T., Milić, N., Jovanović, T., Kovačević, D., Plavšić, B., Stojanović, D., Vidanović, D.,& Ašanin, N.. (2009). Examination of presence of specific antibodies against avian influenza virus in some species of wild birds. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(4), 381-403.
https://doi.org/10.2298/AVB0904381S

Šekler M, Ašanin R, Krnjaić D, Palić T, Milić N, Jovanović T, Kovačević D, Plavšić B, Stojanović D, Vidanović D, Ašanin N. Examination of presence of specific antibodies against avian influenza virus in some species of wild birds. in Acta Veterinaria-Beograd. 2009;59(4):381-403.
doi:10.2298/AVB0904381S .

Šekler, Milanko, Ašanin, Ružica, Krnjaić, Dejan, Palić, T., Milić, Nenad, Jovanović, Tanja, Kovačević, Dragana, Plavšić, B., Stojanović, Dragica, Vidanović, Dejan, Ašanin, N., "Examination of presence of specific antibodies against avian influenza virus in some species of wild birds" in Acta Veterinaria-Beograd, 59, no. 4 (2009):381-403,
https://doi.org/10.2298/AVB0904381S . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Ašanin, Ružica
AU  - Vidić, Branka
AU  - Krnjaić, Dejan
AU  - Petrović, Jelena
AU  - Savić, Sara
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/642
AB  - Listeria monocytogenes is the causative agent of numerous epidemics and sporadic cases of illness in humans. Food is the principal route of infection. Raw materials of animal and vegetable origin are the potential sources of contamination with this bacterium, particularly the foodstuff undergoing minimal processing procedures. However, in the recent years, emphasis has been increasingly laid on the importance of post-processing contamination occurring through the contact of products with contaminated surfaces in food-processing plants, where this bacterium can persist for months, even for years, as a biofilm. Although it appears to be widely accepted that L. monocytogenes forms biofilms on food-processing surfaces, there has been very little direct microscopical evidence to support this. In this study we investigated the ability of biofilm formation in 14 strains of Listeria monocytogenes of different origin. The biofilms were formed on stainless steel surfaces during 7 days of incubation in a tryptone soy broth supplemented with 0.6% yeast extract (TSB-YE) at a temperature of 25ºC. The developed structures were examined using scanning electron microscopy. Our results revealed significant differences among tested Listeria monocytogenes strains in terms of their ability to form a biofilm in given experimental conditions. Seven of 14 investigated strains of Listeria monocytogenes did not form a biofilm, and only individual bacterial cells were distributed over the stainless steel surface. The strains classified as biofilm producers formed structures of different appearances, from a uniform, confluent monolayer of bacterial cells to individual large, three-dimensional cell aggregates. All strains proved weak producers of extracellular polymeric substance.
AB  - Listeria monocytogenes je uzročnik brojnih epidemija i sporadičnih slučajeva oboljenja ljudi, a hrana predstavlja glavni put prenošenja infekcije. Sirovine biljnog i animalnog porekla su jedan od mogućih izvora kontaminacije namirnica ovom bakterijom, što se posebno odnosi na prehrambene proizvode koji prolaze minimalni proces obrade. Međutim, poslednjih godina sve više se ističe značaj postprocesne kontaminacije, nastale kao rezultat kontakta prehrambenih proizvoda sa kontaminiranim površinama u pogonima prehrambene industrije, na kojima ova bakterija može u formi biofilma opstati mesecima, pa čak i godinama. Uprkos skoro opšte prihvaćenom stavu da Listeria monocytogenes formira biofilm, malo je mikroskopskih dokaza koji to potvrđuju. U ovom radu ispitana je sposobnost formiranja biofilma kod 14 sojeva Listeria monocytogenes različitog porekla. Biofilmovi su formirani na površini nerđajućeg čelika za sedam dana inkubacije u tripton soja bujonu sa dodatkom 0.6% kvaščevog ekstrakta na temperaturi od 25ºC. Formirane strukture pregledane su skening elektronskom mikroskopijom. Rezultati pregleda potvrđuju da među testiranim sojevima Listeria monocytogenes postoje značajne razlike u sposobnosti da formiraju biofilm pod korišćenim uslovima ispitivanja. Sedam od 14 testiranih sojeva Listeria monocytogenes nije formiralo biofilm, već su se njihove ćelije kao pojedinačne rasporedile po površini nerđajućeg čelika. Sojevi koji su procenjeni kao 'biofilm producers' formirali su strukture različitog izgleda, od uniformnog, konfluentnog monosloja bakterijskih ćelija do pojedinačnih krupnih, trodimenzionalnih ćelijskih agregata. Svi ispitani sojevi su slabo produkovali ekstracelularnu polimeričnu supstanciju.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Scanning electron microscopy of Listeria monocytogenes biofilms on stainless steel surfaces
T1  - Skening elektronska mikroskopija biofilmova sojeva Listeria monocytogenes na nerđajućem čeliku
VL  - 59
IS  - 4
SP  - 423
EP  - 435
DO  - 10.2298/AVB0904423M
ER  - 

@article{
author = "Milanov, Dubravka and Ašanin, Ružica and Vidić, Branka and Krnjaić, Dejan and Petrović, Jelena and Savić, Sara",
year = "2009",
abstract = "Listeria monocytogenes is the causative agent of numerous epidemics and sporadic cases of illness in humans. Food is the principal route of infection. Raw materials of animal and vegetable origin are the potential sources of contamination with this bacterium, particularly the foodstuff undergoing minimal processing procedures. However, in the recent years, emphasis has been increasingly laid on the importance of post-processing contamination occurring through the contact of products with contaminated surfaces in food-processing plants, where this bacterium can persist for months, even for years, as a biofilm. Although it appears to be widely accepted that L. monocytogenes forms biofilms on food-processing surfaces, there has been very little direct microscopical evidence to support this. In this study we investigated the ability of biofilm formation in 14 strains of Listeria monocytogenes of different origin. The biofilms were formed on stainless steel surfaces during 7 days of incubation in a tryptone soy broth supplemented with 0.6% yeast extract (TSB-YE) at a temperature of 25ºC. The developed structures were examined using scanning electron microscopy. Our results revealed significant differences among tested Listeria monocytogenes strains in terms of their ability to form a biofilm in given experimental conditions. Seven of 14 investigated strains of Listeria monocytogenes did not form a biofilm, and only individual bacterial cells were distributed over the stainless steel surface. The strains classified as biofilm producers formed structures of different appearances, from a uniform, confluent monolayer of bacterial cells to individual large, three-dimensional cell aggregates. All strains proved weak producers of extracellular polymeric substance., Listeria monocytogenes je uzročnik brojnih epidemija i sporadičnih slučajeva oboljenja ljudi, a hrana predstavlja glavni put prenošenja infekcije. Sirovine biljnog i animalnog porekla su jedan od mogućih izvora kontaminacije namirnica ovom bakterijom, što se posebno odnosi na prehrambene proizvode koji prolaze minimalni proces obrade. Međutim, poslednjih godina sve više se ističe značaj postprocesne kontaminacije, nastale kao rezultat kontakta prehrambenih proizvoda sa kontaminiranim površinama u pogonima prehrambene industrije, na kojima ova bakterija može u formi biofilma opstati mesecima, pa čak i godinama. Uprkos skoro opšte prihvaćenom stavu da Listeria monocytogenes formira biofilm, malo je mikroskopskih dokaza koji to potvrđuju. U ovom radu ispitana je sposobnost formiranja biofilma kod 14 sojeva Listeria monocytogenes različitog porekla. Biofilmovi su formirani na površini nerđajućeg čelika za sedam dana inkubacije u tripton soja bujonu sa dodatkom 0.6% kvaščevog ekstrakta na temperaturi od 25ºC. Formirane strukture pregledane su skening elektronskom mikroskopijom. Rezultati pregleda potvrđuju da među testiranim sojevima Listeria monocytogenes postoje značajne razlike u sposobnosti da formiraju biofilm pod korišćenim uslovima ispitivanja. Sedam od 14 testiranih sojeva Listeria monocytogenes nije formiralo biofilm, već su se njihove ćelije kao pojedinačne rasporedile po površini nerđajućeg čelika. Sojevi koji su procenjeni kao 'biofilm producers' formirali su strukture različitog izgleda, od uniformnog, konfluentnog monosloja bakterijskih ćelija do pojedinačnih krupnih, trodimenzionalnih ćelijskih agregata. Svi ispitani sojevi su slabo produkovali ekstracelularnu polimeričnu supstanciju.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Scanning electron microscopy of Listeria monocytogenes biofilms on stainless steel surfaces, Skening elektronska mikroskopija biofilmova sojeva Listeria monocytogenes na nerđajućem čeliku",
volume = "59",
number = "4",
pages = "423-435",
doi = "10.2298/AVB0904423M"
}

Milanov, D., Ašanin, R., Vidić, B., Krnjaić, D., Petrović, J.,& Savić, S.. (2009). Scanning electron microscopy of Listeria monocytogenes biofilms on stainless steel surfaces. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(4), 423-435.
https://doi.org/10.2298/AVB0904423M

Milanov D, Ašanin R, Vidić B, Krnjaić D, Petrović J, Savić S. Scanning electron microscopy of Listeria monocytogenes biofilms on stainless steel surfaces. in Acta Veterinaria-Beograd. 2009;59(4):423-435.
doi:10.2298/AVB0904423M .

Milanov, Dubravka, Ašanin, Ružica, Vidić, Branka, Krnjaić, Dejan, Petrović, Jelena, Savić, Sara, "Scanning electron microscopy of Listeria monocytogenes biofilms on stainless steel surfaces" in Acta Veterinaria-Beograd, 59, no. 4 (2009):423-435,
https://doi.org/10.2298/AVB0904423M . .

TY  - JOUR
AU  - Plavšić, Budimir
AU  - Nedić, Drago
AU  - Mićović, Z.
AU  - Tešić, M.
AU  - Stanojević, S.
AU  - Ašanin, Ružica
AU  - Krnjaić, Dejan
AU  - Tajdić, Nada
AU  - Milanović, S.
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/576
AB  - A prerequisite to the development of an efficient animal health, food safety and traceability management system in the animal food production chain is the implementation of an integrated veterinary informational management system (VIMS) capable for the capture, storage, analysis and retrieval of data and providing the opportunity for the cumulative gathering of the knowledge and capability for its competent interpretation. Such a system will enable collecting appropriate data, including quality management and inspection controls, from all establishments and commodities in the 'from farm to fork' food production chain (farms, holdings, slaughterhouses, laboratories, traders etc.) in a structured, predefined format, and facilitate competent analyses and reporting of such data, as well as the improvement of the existing programs and strategies. The role of information system in animal disease diagnosis, surveillance and notification, control of national and international trade of commodities, food safety management, investigation of diseases, predictive microbiology and quantitative risk assessment is of great importance for the quality of veterinary service. Integral part of the VIMS is animal disease notification system designed according to and in compliance with international requirements, standards and recommendation and able to exchange relevant information with similar information systems. The aim of this contribution is to describe national animal disease notification system which is in place in Serbia as a part of VIMS.
AB  - Preduslov za uspostavljanje efikasnog sistema menadžmenta zdravstvene zaštite životinja, bezbednosti hrane i sledljivosti u lancu proizvodnje hrane je uvođenje integrisanog veterinarskog informacionog menadžment sistema (VIMS) razvijenog na takav način da omogući prikupljanje, čuvanje, analizu i povlačenje podataka i obezbedi kumulativno skupljanje saznanja i njihovu stručnu interpretaciju. Takav sistem će omogućiti prikupljanje podataka, uključujući one iz upravljanja kvalitetom i u okviru inspekcijskih kontrola, od svih objekata u sistemu proizvodnje hrane 'od farme do viljuške' (farme, gazdinstva, klanice, laboratorije, lica koja se bave prometom i dr.) u strukturirano, unapred definisanom obliku, i obezbediti kompetentnu analizu takvih podataka kao i unapređenje postojećih programa i strategija. Uloga informacionih sistema u dijagnostici, nadzoru i prijavljivanju zaraznih bolesti, kontroli unutrašnjeg i međunarodnog prometa, upravljanju bezbednošću hrane, istraživanju žarišta zaraznih bolesti, prediktivnoj mikrobiologiji i kvantitativnoj analizi rizika je od izuzetnog značaja za kvalitet veterinarske službe. Sastavni deo VIMS-a je sistem za prijavljivanje zaraznih bolesti dizajniran prema i u saglasnosti sa međunarodnim zahtevima, standardima i preporukama i osposobljen da vrši razmenu određenih podatka sa sličnim informacionim sistemima. Cilj ovog rada je da se opiše nacionalni sistem za prijavljivanje zaraznih bolesti koji je u Srbiji u primeni kao deo VIMS-a.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Veterinary information management system (VIMS) in the process of notification and management of animal diseases
T1  - Veterinarski informacioni menadžment sistem (VIMS) u procesu prijavljivanja i menadžmenta zaraznih bolesti
VL  - 59
IS  - 1
SP  - 99
EP  - 108
DO  - 10.2298/AVB0901099P
ER  - 

@article{
author = "Plavšić, Budimir and Nedić, Drago and Mićović, Z. and Tešić, M. and Stanojević, S. and Ašanin, Ružica and Krnjaić, Dejan and Tajdić, Nada and Milanović, S.",
year = "2009",
abstract = "A prerequisite to the development of an efficient animal health, food safety and traceability management system in the animal food production chain is the implementation of an integrated veterinary informational management system (VIMS) capable for the capture, storage, analysis and retrieval of data and providing the opportunity for the cumulative gathering of the knowledge and capability for its competent interpretation. Such a system will enable collecting appropriate data, including quality management and inspection controls, from all establishments and commodities in the 'from farm to fork' food production chain (farms, holdings, slaughterhouses, laboratories, traders etc.) in a structured, predefined format, and facilitate competent analyses and reporting of such data, as well as the improvement of the existing programs and strategies. The role of information system in animal disease diagnosis, surveillance and notification, control of national and international trade of commodities, food safety management, investigation of diseases, predictive microbiology and quantitative risk assessment is of great importance for the quality of veterinary service. Integral part of the VIMS is animal disease notification system designed according to and in compliance with international requirements, standards and recommendation and able to exchange relevant information with similar information systems. The aim of this contribution is to describe national animal disease notification system which is in place in Serbia as a part of VIMS., Preduslov za uspostavljanje efikasnog sistema menadžmenta zdravstvene zaštite životinja, bezbednosti hrane i sledljivosti u lancu proizvodnje hrane je uvođenje integrisanog veterinarskog informacionog menadžment sistema (VIMS) razvijenog na takav način da omogući prikupljanje, čuvanje, analizu i povlačenje podataka i obezbedi kumulativno skupljanje saznanja i njihovu stručnu interpretaciju. Takav sistem će omogućiti prikupljanje podataka, uključujući one iz upravljanja kvalitetom i u okviru inspekcijskih kontrola, od svih objekata u sistemu proizvodnje hrane 'od farme do viljuške' (farme, gazdinstva, klanice, laboratorije, lica koja se bave prometom i dr.) u strukturirano, unapred definisanom obliku, i obezbediti kompetentnu analizu takvih podataka kao i unapređenje postojećih programa i strategija. Uloga informacionih sistema u dijagnostici, nadzoru i prijavljivanju zaraznih bolesti, kontroli unutrašnjeg i međunarodnog prometa, upravljanju bezbednošću hrane, istraživanju žarišta zaraznih bolesti, prediktivnoj mikrobiologiji i kvantitativnoj analizi rizika je od izuzetnog značaja za kvalitet veterinarske službe. Sastavni deo VIMS-a je sistem za prijavljivanje zaraznih bolesti dizajniran prema i u saglasnosti sa međunarodnim zahtevima, standardima i preporukama i osposobljen da vrši razmenu određenih podatka sa sličnim informacionim sistemima. Cilj ovog rada je da se opiše nacionalni sistem za prijavljivanje zaraznih bolesti koji je u Srbiji u primeni kao deo VIMS-a.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Veterinary information management system (VIMS) in the process of notification and management of animal diseases, Veterinarski informacioni menadžment sistem (VIMS) u procesu prijavljivanja i menadžmenta zaraznih bolesti",
volume = "59",
number = "1",
pages = "99-108",
doi = "10.2298/AVB0901099P"
}

Plavšić, B., Nedić, D., Mićović, Z., Tešić, M., Stanojević, S., Ašanin, R., Krnjaić, D., Tajdić, N.,& Milanović, S.. (2009). Veterinary information management system (VIMS) in the process of notification and management of animal diseases. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(1), 99-108.
https://doi.org/10.2298/AVB0901099P

Plavšić B, Nedić D, Mićović Z, Tešić M, Stanojević S, Ašanin R, Krnjaić D, Tajdić N, Milanović S. Veterinary information management system (VIMS) in the process of notification and management of animal diseases. in Acta Veterinaria-Beograd. 2009;59(1):99-108.
doi:10.2298/AVB0901099P .

Plavšić, Budimir, Nedić, Drago, Mićović, Z., Tešić, M., Stanojević, S., Ašanin, Ružica, Krnjaić, Dejan, Tajdić, Nada, Milanović, S., "Veterinary information management system (VIMS) in the process of notification and management of animal diseases" in Acta Veterinaria-Beograd, 59, no. 1 (2009):99-108,
https://doi.org/10.2298/AVB0901099P . .

TY  - JOUR
AU  - Ašanin, Ružica
AU  - Žutić, Milenko
AU  - Ašanin, Jelena
AU  - Mišić, Dušan
AU  - Žutić, Jadranka
AU  - Jakić-Dimić, Dobrila
AU  - Milić, Nenad
AU  - Nišavić, Jakov
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/592
AB  - The resistance of bacteria poses a significant problem everywhere in the world, and consequently in our country as well. The non-critical use of antibacterial medicines in human and veterinary medicine has contributed to the spreading of this resistance. Due to the topical importance of this problem, large numbers of states in the world are financing projects of which the objective is to follow and monitor bacterial resistance. The objective of this investigation was to isolate and identify pathogenic strains of E. coli from piglets with clinically manifest diarrhea and to examine the sensitivity of the isolated strains to a certain number of selected antibiotics. The material for these investigations were parts of intestines (jejunum, ileum) from piglets that died, rectal smears and feces of diseased piglets sampled pig farms in the vicinity of Belgrade. Conventional methods of microbiological diagnostics were used for isolation, and conventional and commercial tests API 20E (bio Merieux, France) were used for identification. Following biochemical identification using hyper immune serums for certain group (O) antigens: (O8, O138, O139, O147, O149, and O157), the serological typization of the strains was carried out. Commercial antiserums: T K88 (F4), K99 (F5), and 987P (F6), Toxigenic E. coli pili antisera, Denka Seiken Co. Ltd. Tokyo, Japan) were used to establish the presence and to identify fimbrial antigens-adhesions through slide agglutination reaction. The sensitivity of the antibiotics to the isolated strains of E. coli was examined using the disc diffusion method according to Kirby Bauer and the microdillution method in bouillon according to CLSI recommendations (2008). Examinations using the microdillution method in bouillon were performed with pure active antibiotic substances: ampicillin, apramycin, gentamicin, kanamycin, tetracycline, ceftriaxone, and ciprofloxacin (Sigma, USA). A total of 400 E. coli strains were isolated, including 48 E. coli strains that are within the category of pathogenic strains. Out of the total number of pathogenic strains of E. coli, 32 (66.67%) were found to be multi resistant to 3 or more than 3 antibiotics, and 16 (33.33%) pathogenic strains of E. coli were resistant to 2 or 1 of the examined antibiotics. Resistance to tetracycline and ampicillin was established in 89.58% isolated strains of E. coli, and to ciprofloxacin in 37.5% strains. The lowest percentage of resistance to ceftriaxone was established in 4.17% strains of E. coli. The isolated strains of E. coli were also found to be resistant to certain amino glycosides antibiotics. Thus, resistance to apramycin was established in 18.75% strains whose MIC values were higher than 128 µg/mL. Resistance to kanamycin was established in 52.08% strains of E. coli, and in these, 96.00% strains showed MIC kanamycin values of over 256µg/mL. Resistance to the third amino glycosides antibiotic, gentamicin, was established in 33.33% E. coli strains. All the E. coli strains that led to diarrhea in piglets were resistant to at least two antibiotics, and more than 50% strains were found to be resistant to more than 3 antibiotics. It is significant to stress that all isolated strains of E. coli covered by these investigations were sensitive only to amikacyn. .
AB  - Rezistencija bakterija predstavlja značajan problem svuda u svetu, pa i kod nas. Širenju rezistencije doprinela je nekritička upotreba antibakterijskih lekova u humanoj i veterinarskoj medicini. Zbog aktuelnosti problema veliki broj država finansira projekte čiji je cilj praćenje i nadzor nad rezistencijom bakterija. Cilj ovog istraživanja je bio izolacija i identifikacija patogenih sojeva E. coli kod prasadi sa klinički manifestnom dijarejom i ispitivanje osetljivosti izolovanih sojeva na određeni broj odabranih antibiotika. Materijal za ovo ispitivanje predstavljali su delovi creva (jejunum, ileum) uginule prasadi, rektalni brisevi i feces obolele prasadi uzorkovani na farmama svinja iz okoline Beograda. Za izolaciju su korišćene klasične metode mikrobiološke dijagnostike, a za identifikaciju klasični i komercijalni testovi API 20E (bioMerieux, Francuska). Nakon biohemijske identifikacije pomoću hiperimunih seruma za određene grupne (O) antigene (O8, O138, O139, O147, O149 i O157) vršena je serološka tipizacija sojeva. Za utvrđivanje prisustva i identifikaciju fimbrijalnih antigena - adhezina primenom reakcije aglutinacije na pločici su korišćeni komercijalni antiserumi: K88 (F4), K99 (F5) i 987P (F6), Toxigenic E. coli pili antisera, Denka Seiken Co. Ltd. Tokyo, Japan. Osetljivost na antibiotike izolovanih sojeva E. coli ispitivana je primenom disk difuzione metode po Kirby Baueru i mikrodilucione metode u bujonu prema preporukama CLSI (2008). Ispitivanje mikrodilucionom metodom u bujonu je vršeno sa čistim aktivnim supstancama antibiotika: ampicilina, apramicina, gentamicina, kanamicina, tetraciklina, ceftriaksona i ciprofloksacina (Sigma, SAD). Ukupno je izolovano 400 sojeva E. coli, od kojih je 48 svrstano u kategoriju patogenih sojeva. Od navedenog broja patogenih sojeva E. coli kod 32 (66,67%) je ustanovljena multirezistencija na 3 i više od 3 antibiotika, a kod 16 sojeva (33,33%) rezistencija na 2 ili 1 ispitivani antibiotik. Rezistencija na tetraciklin i ampicilin ustanovljena je kod 89,58% izolovanih sojeva E. coli, a na ciprofloksacin kod 37,5% sojeva. Najniži procenat rezistencije na ceftriakson ustanovljen je kod 4,17% sojeva E. coli. Prisustvo rezistencije kod izolovanih sojeva E. coli ustanovljeno je i na neke aminoglikozidne antibiotike. Tako je rezistencija ustanovljena kod 18,75% sojeva kod kojih su vrednosti MIC-a bile veće od 128 μg/mL. Kod 52,08% sojeva E. coli otkrivena je rezistencija na kanamicin, a od navedenog procenta kod 96,00% sojeva vrednosti MIC-a kanamicina iznosile su preko 256 μg/mL. Takođe je kod 33,33% sojeva E. coli ustanovljena rezistencija i na treći aminoglikozidni antibiotik, gentamicin. Svi sojevi E. coli koji su uzrokovali dijareju kod prasadi bili su rezistentni na najmanje dva antibiotika, a kod preko 50% sojeva otkriveno je prisustvo rezistencije na više od 3 antibiotika. Značajno je naglasiti da su svi izolovani sojevi E. coli obuhvaćeni ispitivanjem bili osetljivi samo na amikacin.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets
T1  - Ispitivanje prisustva novih oblika rezistencije na neke antibiotike kod sojeva E. coli izolovanih od prasadi
VL  - 63
IS  - 5-6
SP  - 311
EP  - 320
DO  - 10.2298/VETGL0906311A
ER  - 

@article{
author = "Ašanin, Ružica and Žutić, Milenko and Ašanin, Jelena and Mišić, Dušan and Žutić, Jadranka and Jakić-Dimić, Dobrila and Milić, Nenad and Nišavić, Jakov",
year = "2009",
abstract = "The resistance of bacteria poses a significant problem everywhere in the world, and consequently in our country as well. The non-critical use of antibacterial medicines in human and veterinary medicine has contributed to the spreading of this resistance. Due to the topical importance of this problem, large numbers of states in the world are financing projects of which the objective is to follow and monitor bacterial resistance. The objective of this investigation was to isolate and identify pathogenic strains of E. coli from piglets with clinically manifest diarrhea and to examine the sensitivity of the isolated strains to a certain number of selected antibiotics. The material for these investigations were parts of intestines (jejunum, ileum) from piglets that died, rectal smears and feces of diseased piglets sampled pig farms in the vicinity of Belgrade. Conventional methods of microbiological diagnostics were used for isolation, and conventional and commercial tests API 20E (bio Merieux, France) were used for identification. Following biochemical identification using hyper immune serums for certain group (O) antigens: (O8, O138, O139, O147, O149, and O157), the serological typization of the strains was carried out. Commercial antiserums: T K88 (F4), K99 (F5), and 987P (F6), Toxigenic E. coli pili antisera, Denka Seiken Co. Ltd. Tokyo, Japan) were used to establish the presence and to identify fimbrial antigens-adhesions through slide agglutination reaction. The sensitivity of the antibiotics to the isolated strains of E. coli was examined using the disc diffusion method according to Kirby Bauer and the microdillution method in bouillon according to CLSI recommendations (2008). Examinations using the microdillution method in bouillon were performed with pure active antibiotic substances: ampicillin, apramycin, gentamicin, kanamycin, tetracycline, ceftriaxone, and ciprofloxacin (Sigma, USA). A total of 400 E. coli strains were isolated, including 48 E. coli strains that are within the category of pathogenic strains. Out of the total number of pathogenic strains of E. coli, 32 (66.67%) were found to be multi resistant to 3 or more than 3 antibiotics, and 16 (33.33%) pathogenic strains of E. coli were resistant to 2 or 1 of the examined antibiotics. Resistance to tetracycline and ampicillin was established in 89.58% isolated strains of E. coli, and to ciprofloxacin in 37.5% strains. The lowest percentage of resistance to ceftriaxone was established in 4.17% strains of E. coli. The isolated strains of E. coli were also found to be resistant to certain amino glycosides antibiotics. Thus, resistance to apramycin was established in 18.75% strains whose MIC values were higher than 128 µg/mL. Resistance to kanamycin was established in 52.08% strains of E. coli, and in these, 96.00% strains showed MIC kanamycin values of over 256µg/mL. Resistance to the third amino glycosides antibiotic, gentamicin, was established in 33.33% E. coli strains. All the E. coli strains that led to diarrhea in piglets were resistant to at least two antibiotics, and more than 50% strains were found to be resistant to more than 3 antibiotics. It is significant to stress that all isolated strains of E. coli covered by these investigations were sensitive only to amikacyn. ., Rezistencija bakterija predstavlja značajan problem svuda u svetu, pa i kod nas. Širenju rezistencije doprinela je nekritička upotreba antibakterijskih lekova u humanoj i veterinarskoj medicini. Zbog aktuelnosti problema veliki broj država finansira projekte čiji je cilj praćenje i nadzor nad rezistencijom bakterija. Cilj ovog istraživanja je bio izolacija i identifikacija patogenih sojeva E. coli kod prasadi sa klinički manifestnom dijarejom i ispitivanje osetljivosti izolovanih sojeva na određeni broj odabranih antibiotika. Materijal za ovo ispitivanje predstavljali su delovi creva (jejunum, ileum) uginule prasadi, rektalni brisevi i feces obolele prasadi uzorkovani na farmama svinja iz okoline Beograda. Za izolaciju su korišćene klasične metode mikrobiološke dijagnostike, a za identifikaciju klasični i komercijalni testovi API 20E (bioMerieux, Francuska). Nakon biohemijske identifikacije pomoću hiperimunih seruma za određene grupne (O) antigene (O8, O138, O139, O147, O149 i O157) vršena je serološka tipizacija sojeva. Za utvrđivanje prisustva i identifikaciju fimbrijalnih antigena - adhezina primenom reakcije aglutinacije na pločici su korišćeni komercijalni antiserumi: K88 (F4), K99 (F5) i 987P (F6), Toxigenic E. coli pili antisera, Denka Seiken Co. Ltd. Tokyo, Japan. Osetljivost na antibiotike izolovanih sojeva E. coli ispitivana je primenom disk difuzione metode po Kirby Baueru i mikrodilucione metode u bujonu prema preporukama CLSI (2008). Ispitivanje mikrodilucionom metodom u bujonu je vršeno sa čistim aktivnim supstancama antibiotika: ampicilina, apramicina, gentamicina, kanamicina, tetraciklina, ceftriaksona i ciprofloksacina (Sigma, SAD). Ukupno je izolovano 400 sojeva E. coli, od kojih je 48 svrstano u kategoriju patogenih sojeva. Od navedenog broja patogenih sojeva E. coli kod 32 (66,67%) je ustanovljena multirezistencija na 3 i više od 3 antibiotika, a kod 16 sojeva (33,33%) rezistencija na 2 ili 1 ispitivani antibiotik. Rezistencija na tetraciklin i ampicilin ustanovljena je kod 89,58% izolovanih sojeva E. coli, a na ciprofloksacin kod 37,5% sojeva. Najniži procenat rezistencije na ceftriakson ustanovljen je kod 4,17% sojeva E. coli. Prisustvo rezistencije kod izolovanih sojeva E. coli ustanovljeno je i na neke aminoglikozidne antibiotike. Tako je rezistencija ustanovljena kod 18,75% sojeva kod kojih su vrednosti MIC-a bile veće od 128 μg/mL. Kod 52,08% sojeva E. coli otkrivena je rezistencija na kanamicin, a od navedenog procenta kod 96,00% sojeva vrednosti MIC-a kanamicina iznosile su preko 256 μg/mL. Takođe je kod 33,33% sojeva E. coli ustanovljena rezistencija i na treći aminoglikozidni antibiotik, gentamicin. Svi sojevi E. coli koji su uzrokovali dijareju kod prasadi bili su rezistentni na najmanje dva antibiotika, a kod preko 50% sojeva otkriveno je prisustvo rezistencije na više od 3 antibiotika. Značajno je naglasiti da su svi izolovani sojevi E. coli obuhvaćeni ispitivanjem bili osetljivi samo na amikacin.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets, Ispitivanje prisustva novih oblika rezistencije na neke antibiotike kod sojeva E. coli izolovanih od prasadi",
volume = "63",
number = "5-6",
pages = "311-320",
doi = "10.2298/VETGL0906311A"
}

Ašanin, R., Žutić, M., Ašanin, J., Mišić, D., Žutić, J., Jakić-Dimić, D., Milić, N.,& Nišavić, J.. (2009). The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(5-6), 311-320.
https://doi.org/10.2298/VETGL0906311A

Ašanin R, Žutić M, Ašanin J, Mišić D, Žutić J, Jakić-Dimić D, Milić N, Nišavić J. The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets. in Veterinarski Glasnik. 2009;63(5-6):311-320.
doi:10.2298/VETGL0906311A .

Ašanin, Ružica, Žutić, Milenko, Ašanin, Jelena, Mišić, Dušan, Žutić, Jadranka, Jakić-Dimić, Dobrila, Milić, Nenad, Nišavić, Jakov, "The investigation of new forms of resistance to some antibiotics in E coli strains isolated from piglets" in Veterinarski Glasnik, 63, no. 5-6 (2009):311-320,
https://doi.org/10.2298/VETGL0906311A . .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Ašanin, Ružica
AU  - Ivanović, Jasna
AU  - Žižović, Irena
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/631
AB  - The multiresistance of bacteria to antibiotics, as well as the lack of new antibiotics on the market encouraged the research of antibacterial activity of non-antibiotic substances including plant extracts. During the previous decades, it has been proven that extracts of certain plants have a strong antibacterial activity, but their clinical use was limited due to the presence of organic solvents. However, plant extracts obtained by the process of supercritical fluid extraction contain no traces of solvents, and the latest researches have established that they do have antibacterial effects on some gram-positive bacteria. This comparative study included extracts of Common Mullein, Angelica and Echinacea obtained by means of supercritical fluid extraction, Soxlet extraction and ultrasound-assisted extraction. The study of their antibacterial activity was performed on some strains of Staphylococcus, Enterobacter cloacae and E. coli isolated from clinical material of human and animal origin. A referential strain of S. aureus ATCC 25923 was included in the research. In the study broth macrodilution method was applied by which the MIC values of extracts were determined. The Angelica extract obtained by ultrasound-assisted extraction had the strongest antibacterial activity, i.e. the lowest MIC value of 40 μg/mL for S. epidermidis strain. The Angelica extract obtained by supercritical fluid extraction also showed substantial antibacterial activity to all Staphylococcus strains included in this study, with the MIC values of 320 to 640 μg/mL. The extracts of Echinacea and Common Mullein obtained by supercritical fluid extraction, as well as of Echinacea extract obtained by Soxlet extraction showed no antibacterial activity since the MIC values of these extracts were 2560 μg/mL or >2560 μg/mL for all bacterial strains icluded in the study.
AB  - Multirezistencija na antibiotike bakterija kao i nedostatak novih antibiotika na tržištu lekova podstakao je ispitivanja antibakterijskog delovanja supstancija koje nisu antibiotici uključujući i biljne ekstrakte. Tokom prethodnih decenija, dokazano je da ekstrakti pojedinih biljaka imaju izraženo antibakterijsko delovanje ali je zbog prisustva tragova organskih rastvarača, njihova klinička upotreba ogranič ena. Ekstrakti biljaka dobijeni procesom natkritične ekstrakcije, međutim, nemaju tragove rastvarača, a na osnovu dosadašnjih ispitivanja je utvrđeno da deluju na neke gram-pozitivne bakterije. Ovim komparativinim ispitivanjem obuhvaćeni su ekstrakti divizme, anđelike i ehinacee dobijeni procesima natkritične ekstrakcije, Soxlet-ove ekstrakcije i ultrazvučne ekstrakcije. Ispitivanje njihovog antibakterijskog delovanja vršeno je na nekim sojevima stafilokoka, Enterobacter cloacae i E. coli izolovanim iz uzoraka kliničkog materijala poreklom od životinja i ljudi. U ispitivanje je bio uključen i referentni soj S. aureus ATCC 25923. Za ispitivanje antibakterijskog delovanja ekstrakata primenjen je makrodilucioni metod u bujonu pomoću koga su određivane vrednosti MIC ekstrakata. Najjače antibakterijsko delovanje, odnosno najnižu vrednost MIC od 40 μg/mL za soj S. epidermidis imao je ekstrakt anđelike dobijen ultrazvučnom ekstrakcijom. Ekstrakt anđelike dobijen natkritičnom ekstrakcijom je takođe pokazao značajno antibakterijsko delovanje na sve sojeve stafilokoka obuhvaćenih ispitivanjem sa vrednostima MIC od 320 do 640 μg/mL. Ispitivanjem ekstrakata ehinacee i divizme dobijenih procesom nadkritične ekstrakcije kao i ekstrakta ehinacee dobijenog Soxlet-ovom ekstrakcijom, nije utvrđeno antibakterijsko delovanje jer je vrednost MIC ovih ekstrakata iznosila 2560 μg/mL ili >2560 μg/mL za sojeve bakterija koji su obuhvaćeni ispitivanjem.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Investigation of antibacterial activity of supercritical extracts of plants, as well as of extracts obtained by other technological processes on some bacteria isolated from animals
T1  - Ispitivanje antibakterijskog delovanja natkritičnih ekstrakata biljaka kao i ekstrakata dobijenih drugim tehnološkim procesima na neke bakterije izolovane od životinja
VL  - 59
IS  - 5-6
SP  - 557
EP  - 568
DO  - 10.2298/AVB0906557M
ER  - 

@article{
author = "Mišić, Dušan and Ašanin, Ružica and Ivanović, Jasna and Žižović, Irena",
year = "2009",
abstract = "The multiresistance of bacteria to antibiotics, as well as the lack of new antibiotics on the market encouraged the research of antibacterial activity of non-antibiotic substances including plant extracts. During the previous decades, it has been proven that extracts of certain plants have a strong antibacterial activity, but their clinical use was limited due to the presence of organic solvents. However, plant extracts obtained by the process of supercritical fluid extraction contain no traces of solvents, and the latest researches have established that they do have antibacterial effects on some gram-positive bacteria. This comparative study included extracts of Common Mullein, Angelica and Echinacea obtained by means of supercritical fluid extraction, Soxlet extraction and ultrasound-assisted extraction. The study of their antibacterial activity was performed on some strains of Staphylococcus, Enterobacter cloacae and E. coli isolated from clinical material of human and animal origin. A referential strain of S. aureus ATCC 25923 was included in the research. In the study broth macrodilution method was applied by which the MIC values of extracts were determined. The Angelica extract obtained by ultrasound-assisted extraction had the strongest antibacterial activity, i.e. the lowest MIC value of 40 μg/mL for S. epidermidis strain. The Angelica extract obtained by supercritical fluid extraction also showed substantial antibacterial activity to all Staphylococcus strains included in this study, with the MIC values of 320 to 640 μg/mL. The extracts of Echinacea and Common Mullein obtained by supercritical fluid extraction, as well as of Echinacea extract obtained by Soxlet extraction showed no antibacterial activity since the MIC values of these extracts were 2560 μg/mL or >2560 μg/mL for all bacterial strains icluded in the study., Multirezistencija na antibiotike bakterija kao i nedostatak novih antibiotika na tržištu lekova podstakao je ispitivanja antibakterijskog delovanja supstancija koje nisu antibiotici uključujući i biljne ekstrakte. Tokom prethodnih decenija, dokazano je da ekstrakti pojedinih biljaka imaju izraženo antibakterijsko delovanje ali je zbog prisustva tragova organskih rastvarača, njihova klinička upotreba ogranič ena. Ekstrakti biljaka dobijeni procesom natkritične ekstrakcije, međutim, nemaju tragove rastvarača, a na osnovu dosadašnjih ispitivanja je utvrđeno da deluju na neke gram-pozitivne bakterije. Ovim komparativinim ispitivanjem obuhvaćeni su ekstrakti divizme, anđelike i ehinacee dobijeni procesima natkritične ekstrakcije, Soxlet-ove ekstrakcije i ultrazvučne ekstrakcije. Ispitivanje njihovog antibakterijskog delovanja vršeno je na nekim sojevima stafilokoka, Enterobacter cloacae i E. coli izolovanim iz uzoraka kliničkog materijala poreklom od životinja i ljudi. U ispitivanje je bio uključen i referentni soj S. aureus ATCC 25923. Za ispitivanje antibakterijskog delovanja ekstrakata primenjen je makrodilucioni metod u bujonu pomoću koga su određivane vrednosti MIC ekstrakata. Najjače antibakterijsko delovanje, odnosno najnižu vrednost MIC od 40 μg/mL za soj S. epidermidis imao je ekstrakt anđelike dobijen ultrazvučnom ekstrakcijom. Ekstrakt anđelike dobijen natkritičnom ekstrakcijom je takođe pokazao značajno antibakterijsko delovanje na sve sojeve stafilokoka obuhvaćenih ispitivanjem sa vrednostima MIC od 320 do 640 μg/mL. Ispitivanjem ekstrakata ehinacee i divizme dobijenih procesom nadkritične ekstrakcije kao i ekstrakta ehinacee dobijenog Soxlet-ovom ekstrakcijom, nije utvrđeno antibakterijsko delovanje jer je vrednost MIC ovih ekstrakata iznosila 2560 μg/mL ili >2560 μg/mL za sojeve bakterija koji su obuhvaćeni ispitivanjem.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Investigation of antibacterial activity of supercritical extracts of plants, as well as of extracts obtained by other technological processes on some bacteria isolated from animals, Ispitivanje antibakterijskog delovanja natkritičnih ekstrakata biljaka kao i ekstrakata dobijenih drugim tehnološkim procesima na neke bakterije izolovane od životinja",
volume = "59",
number = "5-6",
pages = "557-568",
doi = "10.2298/AVB0906557M"
}

Mišić, D., Ašanin, R., Ivanović, J.,& Žižović, I.. (2009). Investigation of antibacterial activity of supercritical extracts of plants, as well as of extracts obtained by other technological processes on some bacteria isolated from animals. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(5-6), 557-568.
https://doi.org/10.2298/AVB0906557M

Mišić D, Ašanin R, Ivanović J, Žižović I. Investigation of antibacterial activity of supercritical extracts of plants, as well as of extracts obtained by other technological processes on some bacteria isolated from animals. in Acta Veterinaria-Beograd. 2009;59(5-6):557-568.
doi:10.2298/AVB0906557M .

Mišić, Dušan, Ašanin, Ružica, Ivanović, Jasna, Žižović, Irena, "Investigation of antibacterial activity of supercritical extracts of plants, as well as of extracts obtained by other technological processes on some bacteria isolated from animals" in Acta Veterinaria-Beograd, 59, no. 5-6 (2009):557-568,
https://doi.org/10.2298/AVB0906557M . .

TY  - JOUR
AU  - Žižović, Irena
AU  - Mišić, Dušan
AU  - Ašanin, Ružica
AU  - Ivanović, Jasna
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/572
AB  - In the present study, isolation of essential oils from leaves of rosemary (Rosmarinus officinalis), sage (Salvia officinalis) and thyme (Thymus vulgaris) by supercritical carbon dioxide as well as hydrodistillation was investigated from the point of yield and antimicrobial activity of obtained extracts against Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis and E.coli strains. Thyme isolates had the highest antibacterial efficiency against tested foodborne bacteria strains (MIC=40-640 μg/cm3) followed by rosemary (MIC=320-1280 μg/cm3) and sage (MIC=160-2560 μg/cm3) isolates. Among tested bacteria, Bacillus species were the most sensitive to presence of all tested supercritical extracts and hydrodistillates. .
AB  - U ovoj studiji je ispitana izolacija etarskih ulja iz lista ruzmarina (Rosmarinus officinalis), žalfije (Salvia officinalis) i timijana (Thymus vulgaris) postupkom natkritične ekstrakcije ugljenik(IV)-oksidom sa aspekta prinosa i antimikrobne aktivnosti dobijenih izolata protiv Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis i E.coli sojeva. Izolati timijana su pokazali najjače antibakterijsko dejstvo u odnosu na testirane sojeve (MIC=40-640 μg/cm3). Zatim slede izolati ruzmarina (MIC=320-1280 μg/cm3) i žalfije (MIC=160-2560 μg/cm3). Među testiranim bakterijama, Bacillus sojevi su se pokazali kao najosetljiviji na prisustvo kako natkritičnih ekstrakata, tako i hidrodestilata. .
PB  - Univerzitet u Nišu - Tehnološki fakultet, Leskovac
T2  - Zbornik radova Tehnološkog fakulteta, Leskovac
T1  - Antibacterial activity of essential oils of some Lamiaceae family species isolated by different methods
T1  - Antibakterijska aktivnost etarskih ulja odabranih biljaka familije Lamiaceae izolovanih različitim metodama
IS  - 19
SP  - 20
EP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_572
ER  - 

@article{
author = "Žižović, Irena and Mišić, Dušan and Ašanin, Ružica and Ivanović, Jasna",
year = "2009",
abstract = "In the present study, isolation of essential oils from leaves of rosemary (Rosmarinus officinalis), sage (Salvia officinalis) and thyme (Thymus vulgaris) by supercritical carbon dioxide as well as hydrodistillation was investigated from the point of yield and antimicrobial activity of obtained extracts against Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis and E.coli strains. Thyme isolates had the highest antibacterial efficiency against tested foodborne bacteria strains (MIC=40-640 μg/cm3) followed by rosemary (MIC=320-1280 μg/cm3) and sage (MIC=160-2560 μg/cm3) isolates. Among tested bacteria, Bacillus species were the most sensitive to presence of all tested supercritical extracts and hydrodistillates. ., U ovoj studiji je ispitana izolacija etarskih ulja iz lista ruzmarina (Rosmarinus officinalis), žalfije (Salvia officinalis) i timijana (Thymus vulgaris) postupkom natkritične ekstrakcije ugljenik(IV)-oksidom sa aspekta prinosa i antimikrobne aktivnosti dobijenih izolata protiv Geobacillus stearotermophillus, Bacillus cereus, Bacillus subtilis var. niger, Enterococcus faecium, Salmonella enteritidis i E.coli sojeva. Izolati timijana su pokazali najjače antibakterijsko dejstvo u odnosu na testirane sojeve (MIC=40-640 μg/cm3). Zatim slede izolati ruzmarina (MIC=320-1280 μg/cm3) i žalfije (MIC=160-2560 μg/cm3). Među testiranim bakterijama, Bacillus sojevi su se pokazali kao najosetljiviji na prisustvo kako natkritičnih ekstrakata, tako i hidrodestilata. .",
publisher = "Univerzitet u Nišu - Tehnološki fakultet, Leskovac",
journal = "Zbornik radova Tehnološkog fakulteta, Leskovac",
title = "Antibacterial activity of essential oils of some Lamiaceae family species isolated by different methods, Antibakterijska aktivnost etarskih ulja odabranih biljaka familije Lamiaceae izolovanih različitim metodama",
number = "19",
pages = "20-26",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_572"
}

Žižović, I., Mišić, D., Ašanin, R.,& Ivanović, J.. (2009). Antibacterial activity of essential oils of some Lamiaceae family species isolated by different methods. in Zbornik radova Tehnološkog fakulteta, Leskovac
Univerzitet u Nišu - Tehnološki fakultet, Leskovac.(19), 20-26.
https://hdl.handle.net/21.15107/rcub_veterinar_572

Žižović I, Mišić D, Ašanin R, Ivanović J. Antibacterial activity of essential oils of some Lamiaceae family species isolated by different methods. in Zbornik radova Tehnološkog fakulteta, Leskovac. 2009;(19):20-26.
https://hdl.handle.net/21.15107/rcub_veterinar_572 .

Žižović, Irena, Mišić, Dušan, Ašanin, Ružica, Ivanović, Jasna, "Antibacterial activity of essential oils of some Lamiaceae family species isolated by different methods" in Zbornik radova Tehnološkog fakulteta, Leskovac, no. 19 (2009):20-26,
https://hdl.handle.net/21.15107/rcub_veterinar_572 .

TY  - JOUR
AU  - Matović, Kazimir
AU  - Ašanin, Ružica
AU  - Radojičić, Sonja
AU  - Lako, Branislav
AU  - Žarković, Aleksandar
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/564
AB  - The most reliable diagnosis of an infectuous disease is confirmed by isolation of its pathogen. When it comes to brucellosis, it is important to know that brucella isolation is rarely successful; it is not only very complicated but is as well hazardous for laboratory workers. Due to the above mentioned reasons, it is reasonable to use serological tests for routine diagnosis of this zoonose. This paper deals with examination of bovine sera samples with the aim to detect the titer of specific antibodies against brucellosis. In order to choose and evaluate properly the best test in terms of applicability, speed of performance, and provision of correct results, five serological tests were assayed: rapid serum plate agglutination (Rose Bengal test), Brucella abortus bovis test (RB, BAB test); serum agglutination test (titration) - by Wright, as micro method (mSAT); reaction of complement fixation, and also as micro method (mCF); indirect imunoenzyime test (iELISA) and competitive imunoenzyme test (cELISA). This paper includes 630 samples of bovine blood sera, as well as positive and negative international antibrucella serum as the mandatory control. The presence of specific antibodies against brucella was determined in 125 samples of bovine blood sera. Based on the analysis of the results obtained, evaluation of sensitivity and specificity of these tests was conducted. iELISA and RB test proved to be the most sensitive, while the highest specificity was determined in mCF, and less specific were mSAT and iELISA. RB test had the lowest specificity.
AB  - Najsigurnija dijagnostika bolesti infektivnog karaktera vrši se izolacijom uzroč nika. Kada je bruceloza u pitanju, mora da se ima u vidu, da je izolacija brucela uspešna u niskom procentu, veoma komplikovana i predstavlja opasnost po laboratorijske radnike. Zbog navedenih razloga, primena seroloških testova u rutinskoj dijagnostici ove zoonoze je opravdana. U ovom radu su vršena ispitivanja uzoraka seruma goveda radi određivanja prisustva titra specifičnih antitela protiv brucela, u svrhu dijagnostikovanja bruceloze. U nameri da se pravilno izbabere i proceni najbolji test u smislu aplikativnosti, brzine izvođenja i dobijanja pouzdanih rezultata, tokom ispitivanja je korišćeno pet seroloških testova: brza serumska aglutinacija na pločici - Rose Bengal test - Brucella abortus bovis test (RB, BAB test); spora aglutinacija (u titraciji) - metoda po Wrightu, kao mikrometoda (mSAT); reakcija vezivanja komplementa, takođe kao mikrometoda (mRVK); indirektni imunoenzimski test (iELISA) i kompetitivni imunoenzimski test (cELISA). Ispitivanjem je bilo obuhvaćeno 630 uzoraka krvnog seruma goveda kao i pozitivni i negativni internacionalni antibrucela serumi kao obavezne kontrole. Prisustvo specifičnih antitela protiv brucela vrsta ustanovljeno je u 125 uzoraka krvnog seruma goveda. Na osnovu analize dobijenih rezultata vršena je procena osetljivosti i specifičnosti navedenih testova. Najosetljivijim su se pokazali iELISA i BAB test, a najveća specifičnost je ustanovljena kod mRVK. Manje specifični bili su mSAT i iELISA, a najmanja specifičnost je ustanovljena kod BAB testa.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Examination of sensitivity and specificity of some serological tests in diagnostics of bovine brucellosis
T1  - Ispitivanje osetljivosti i specifičnosti nekih seroloških testova u dijagnostici bruceloze kod goveda
VL  - 58
IS  - 5-6
SP  - 467
EP  - 476
DO  - 10.2298/AVB0806467M
ER  - 

@article{
author = "Matović, Kazimir and Ašanin, Ružica and Radojičić, Sonja and Lako, Branislav and Žarković, Aleksandar",
year = "2008",
abstract = "The most reliable diagnosis of an infectuous disease is confirmed by isolation of its pathogen. When it comes to brucellosis, it is important to know that brucella isolation is rarely successful; it is not only very complicated but is as well hazardous for laboratory workers. Due to the above mentioned reasons, it is reasonable to use serological tests for routine diagnosis of this zoonose. This paper deals with examination of bovine sera samples with the aim to detect the titer of specific antibodies against brucellosis. In order to choose and evaluate properly the best test in terms of applicability, speed of performance, and provision of correct results, five serological tests were assayed: rapid serum plate agglutination (Rose Bengal test), Brucella abortus bovis test (RB, BAB test); serum agglutination test (titration) - by Wright, as micro method (mSAT); reaction of complement fixation, and also as micro method (mCF); indirect imunoenzyime test (iELISA) and competitive imunoenzyme test (cELISA). This paper includes 630 samples of bovine blood sera, as well as positive and negative international antibrucella serum as the mandatory control. The presence of specific antibodies against brucella was determined in 125 samples of bovine blood sera. Based on the analysis of the results obtained, evaluation of sensitivity and specificity of these tests was conducted. iELISA and RB test proved to be the most sensitive, while the highest specificity was determined in mCF, and less specific were mSAT and iELISA. RB test had the lowest specificity., Najsigurnija dijagnostika bolesti infektivnog karaktera vrši se izolacijom uzroč nika. Kada je bruceloza u pitanju, mora da se ima u vidu, da je izolacija brucela uspešna u niskom procentu, veoma komplikovana i predstavlja opasnost po laboratorijske radnike. Zbog navedenih razloga, primena seroloških testova u rutinskoj dijagnostici ove zoonoze je opravdana. U ovom radu su vršena ispitivanja uzoraka seruma goveda radi određivanja prisustva titra specifičnih antitela protiv brucela, u svrhu dijagnostikovanja bruceloze. U nameri da se pravilno izbabere i proceni najbolji test u smislu aplikativnosti, brzine izvođenja i dobijanja pouzdanih rezultata, tokom ispitivanja je korišćeno pet seroloških testova: brza serumska aglutinacija na pločici - Rose Bengal test - Brucella abortus bovis test (RB, BAB test); spora aglutinacija (u titraciji) - metoda po Wrightu, kao mikrometoda (mSAT); reakcija vezivanja komplementa, takođe kao mikrometoda (mRVK); indirektni imunoenzimski test (iELISA) i kompetitivni imunoenzimski test (cELISA). Ispitivanjem je bilo obuhvaćeno 630 uzoraka krvnog seruma goveda kao i pozitivni i negativni internacionalni antibrucela serumi kao obavezne kontrole. Prisustvo specifičnih antitela protiv brucela vrsta ustanovljeno je u 125 uzoraka krvnog seruma goveda. Na osnovu analize dobijenih rezultata vršena je procena osetljivosti i specifičnosti navedenih testova. Najosetljivijim su se pokazali iELISA i BAB test, a najveća specifičnost je ustanovljena kod mRVK. Manje specifični bili su mSAT i iELISA, a najmanja specifičnost je ustanovljena kod BAB testa.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Examination of sensitivity and specificity of some serological tests in diagnostics of bovine brucellosis, Ispitivanje osetljivosti i specifičnosti nekih seroloških testova u dijagnostici bruceloze kod goveda",
volume = "58",
number = "5-6",
pages = "467-476",
doi = "10.2298/AVB0806467M"
}

Matović, K., Ašanin, R., Radojičić, S., Lako, B.,& Žarković, A.. (2008). Examination of sensitivity and specificity of some serological tests in diagnostics of bovine brucellosis. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(5-6), 467-476.
https://doi.org/10.2298/AVB0806467M

Matović K, Ašanin R, Radojičić S, Lako B, Žarković A. Examination of sensitivity and specificity of some serological tests in diagnostics of bovine brucellosis. in Acta Veterinaria-Beograd. 2008;58(5-6):467-476.
doi:10.2298/AVB0806467M .

Matović, Kazimir, Ašanin, Ružica, Radojičić, Sonja, Lako, Branislav, Žarković, Aleksandar, "Examination of sensitivity and specificity of some serological tests in diagnostics of bovine brucellosis" in Acta Veterinaria-Beograd, 58, no. 5-6 (2008):467-476,
https://doi.org/10.2298/AVB0806467M . .

TY  - JOUR
AU  - Stojanov, Igor
AU  - Milić, Nenad
AU  - Ašanin, Ružica
AU  - Vidić, Branka
AU  - Nišavić, Jakov
AU  - Grgić, Ž.
AU  - Prica, Nadežda
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/553
AB  - Campylobacteria are widely distributed in domestic and wild animals. Avian species are the most common host of campylobacteria, thus an important source for human infection caused by this organism. Campylobacteriosis in animals frequently proceeds without clinical symptoms, which may be due to the different virulence of the agent, as well as to the immune status of the infected animal. In this trial we investigated the effects of the vaccine against infective bursal disease (IBD) on occurrence and clinical manifestation of campylobacteriosis in conditions of controlled experimental infection with the strain Campylobacter jejuni. The immunosuppressive effects of a live attenuated vaccine against IBD on development of clinically manifest campylobacteriosis in perorally challenged chickens were assessed. The investigation was performed on 90 chickens in experimental conditions. The presence of Campylobacter jejuni was confirmed by reisolation, while identification of the organism was performed on the basis of its morphological, cultural and biochemical characteristics. Specific antibody titer and humoral immune response to C. jejuni specific antibodies were monitored using the complement fixation reaction method. Clinical symptoms of the disease were observed in chickens infected with Campylobacter jejuni and vaccinated against infective bursal disease. Pathomorphological findings revealed changes in the intestines and liver, from which Campylobacter jejuni was isolated. C. jejuni specific antibodies were detected in the infected birds in the third week post infection, with titer values ranging from 1:4 to 1:16. Results of our research strongly imply an immunosuppressive effect of the IBD vaccine on development of campylobacteriosis, which is supported by the clinical and morphological findings, i.e. isolation of the agent and detection of specific antibodies.
AB  - Kampilobakterije su kod domaćih i divljih životinja široko rasprostranjeni mikroorganizmi, a živina je jedan od glavnih nosilaca kampilobakterija i važan izvor infekcija ljudi. Klinički simptomi kampilobakterioze kod živine se relativno retko javljaju, a razloge treba tražiti u različitoj virulenciji uzročnika i u imunološkom statusu inficiranih jedniki. U ovom radu je ispitivan uticaj vakcine protiv infektivnog burzitisa živine na nastanak i kliničku pojavu kampilobakterioze, posle veštačke infekcije terenskim sojem Campylobacter jejuni. Ispitivanje je izvršeno na 90 pilića u eksperimentalnim uslovima. Prisustvo Campylobacter jejuni u organizmu veštački inficiranih pilića dokazivano je reizolacijom uzročnika, a identifikacija izolovanih bakterija vršena je na osnovu njihovih morfoloških, kulturelnih i biohemijskih osobina. Prisustvo titra specifičnih antitela i kretanje humoralnog imunskog odgovora na antigene Campylobacter jejuni kod pilića u ogledu praćeno je primenom reakcije vezivanja komplementa (RVK). Kod pilića inficiranih sojem Campylobacter jejuni i imunizovanih vakcinom protiv infektivnog burzitisa uočeni su klinički znaci bolesti. Patomorfološkim ispitivanjem utvrđene su promene na crevima i jetri pilića iz kojih je izolovan Campylobacter jejuni. Specifična antitela za C. jejuni dokazana su kod inficiranih jedinki u trećoj nedelji od veštačke infekcije, a vrednosti njihovog titra su iznosile od 1:4 do 1:16. Rezultati ovih ispitivanja su potvrdili imunospresivno delovanje vakcine protiv infektivnog burzitisa koje je imalo za rezultat klinički manifestnu kampilobakteriozu. Infekcija je potvrđena na osnovu kliničkih simptoma i patomorfoloških promena, izolacijom uzročnika, kao i na osnovu vrednosti titra specifičnih antitela utvrđenih u uzorcima krvnih seruma oglednih pilića.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effects of some immunosuppressive factors on campylobacteriosis outbreaks in poultry
T1  - Ispitivanje uticaja nekih imunosupresivnih faktora na pojavu kampilobakterioze živine
VL  - 58
IS  - 2-3
SP  - 167
EP  - 177
DO  - 10.2298/AVB0803167S
ER  - 

@article{
author = "Stojanov, Igor and Milić, Nenad and Ašanin, Ružica and Vidić, Branka and Nišavić, Jakov and Grgić, Ž. and Prica, Nadežda",
year = "2008",
abstract = "Campylobacteria are widely distributed in domestic and wild animals. Avian species are the most common host of campylobacteria, thus an important source for human infection caused by this organism. Campylobacteriosis in animals frequently proceeds without clinical symptoms, which may be due to the different virulence of the agent, as well as to the immune status of the infected animal. In this trial we investigated the effects of the vaccine against infective bursal disease (IBD) on occurrence and clinical manifestation of campylobacteriosis in conditions of controlled experimental infection with the strain Campylobacter jejuni. The immunosuppressive effects of a live attenuated vaccine against IBD on development of clinically manifest campylobacteriosis in perorally challenged chickens were assessed. The investigation was performed on 90 chickens in experimental conditions. The presence of Campylobacter jejuni was confirmed by reisolation, while identification of the organism was performed on the basis of its morphological, cultural and biochemical characteristics. Specific antibody titer and humoral immune response to C. jejuni specific antibodies were monitored using the complement fixation reaction method. Clinical symptoms of the disease were observed in chickens infected with Campylobacter jejuni and vaccinated against infective bursal disease. Pathomorphological findings revealed changes in the intestines and liver, from which Campylobacter jejuni was isolated. C. jejuni specific antibodies were detected in the infected birds in the third week post infection, with titer values ranging from 1:4 to 1:16. Results of our research strongly imply an immunosuppressive effect of the IBD vaccine on development of campylobacteriosis, which is supported by the clinical and morphological findings, i.e. isolation of the agent and detection of specific antibodies., Kampilobakterije su kod domaćih i divljih životinja široko rasprostranjeni mikroorganizmi, a živina je jedan od glavnih nosilaca kampilobakterija i važan izvor infekcija ljudi. Klinički simptomi kampilobakterioze kod živine se relativno retko javljaju, a razloge treba tražiti u različitoj virulenciji uzročnika i u imunološkom statusu inficiranih jedniki. U ovom radu je ispitivan uticaj vakcine protiv infektivnog burzitisa živine na nastanak i kliničku pojavu kampilobakterioze, posle veštačke infekcije terenskim sojem Campylobacter jejuni. Ispitivanje je izvršeno na 90 pilića u eksperimentalnim uslovima. Prisustvo Campylobacter jejuni u organizmu veštački inficiranih pilića dokazivano je reizolacijom uzročnika, a identifikacija izolovanih bakterija vršena je na osnovu njihovih morfoloških, kulturelnih i biohemijskih osobina. Prisustvo titra specifičnih antitela i kretanje humoralnog imunskog odgovora na antigene Campylobacter jejuni kod pilića u ogledu praćeno je primenom reakcije vezivanja komplementa (RVK). Kod pilića inficiranih sojem Campylobacter jejuni i imunizovanih vakcinom protiv infektivnog burzitisa uočeni su klinički znaci bolesti. Patomorfološkim ispitivanjem utvrđene su promene na crevima i jetri pilića iz kojih je izolovan Campylobacter jejuni. Specifična antitela za C. jejuni dokazana su kod inficiranih jedinki u trećoj nedelji od veštačke infekcije, a vrednosti njihovog titra su iznosile od 1:4 do 1:16. Rezultati ovih ispitivanja su potvrdili imunospresivno delovanje vakcine protiv infektivnog burzitisa koje je imalo za rezultat klinički manifestnu kampilobakteriozu. Infekcija je potvrđena na osnovu kliničkih simptoma i patomorfoloških promena, izolacijom uzročnika, kao i na osnovu vrednosti titra specifičnih antitela utvrđenih u uzorcima krvnih seruma oglednih pilića.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effects of some immunosuppressive factors on campylobacteriosis outbreaks in poultry, Ispitivanje uticaja nekih imunosupresivnih faktora na pojavu kampilobakterioze živine",
volume = "58",
number = "2-3",
pages = "167-177",
doi = "10.2298/AVB0803167S"
}

Stojanov, I., Milić, N., Ašanin, R., Vidić, B., Nišavić, J., Grgić, Ž.,& Prica, N.. (2008). Effects of some immunosuppressive factors on campylobacteriosis outbreaks in poultry. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(2-3), 167-177.
https://doi.org/10.2298/AVB0803167S

Stojanov I, Milić N, Ašanin R, Vidić B, Nišavić J, Grgić Ž, Prica N. Effects of some immunosuppressive factors on campylobacteriosis outbreaks in poultry. in Acta Veterinaria-Beograd. 2008;58(2-3):167-177.
doi:10.2298/AVB0803167S .

Stojanov, Igor, Milić, Nenad, Ašanin, Ružica, Vidić, Branka, Nišavić, Jakov, Grgić, Ž., Prica, Nadežda, "Effects of some immunosuppressive factors on campylobacteriosis outbreaks in poultry" in Acta Veterinaria-Beograd, 58, no. 2-3 (2008):167-177,
https://doi.org/10.2298/AVB0803167S . .

TY  - JOUR
AU  - Žutić, Milenko
AU  - Ašanin, Ružica
AU  - Milić, Nenad
AU  - Ivetić, V.
AU  - Vidić, Branka
AU  - Žutić, Jadranka
AU  - Ašanin, Jelena
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/555
AB  - The presence of Actinobacillus pleuropneumoniae has been established in all suspected cases of pleuropneumonia at several farms that have been included in the research. Equal incidence of pleuropneumonia has been found both among piglets for breeding and for fattening. The health monitoring of herds is extremely important, firstly because of the need for the adequate strategy to be chosen for controlling the Actinobacillus-caused pleuropneumonia and, at the same time, in order to prevent enormous economic losses that this disease may cause. The morphological, physiological and biochemical characteristics of isolated strains of Actinobacillus pleuropneumoniae were completely identical to those of referent strains. It has been found that Actinobacillus pleuropneumoniae grows most abundantly on chocolate agar when PolyVitex (bioMerieux) is used as the substrate. Out of 237 samples of altered parts of swine lungs, 13 bacterial species have been found in 193 (81%), and the incidence of Actinobacillus pleuropneumoniae within this percentage was 33%. Of all bacterial species isolated in pure culture from all investigated specimen the most dominant species were Pasteurella multocida with the incidence of 32.64% and Actinobacillus pleuropneumoniae with 29.01%. Their common incidence in all positive findings was 61.65%. If one adds to this their participation in mixed infections (2.59%), this percentage is higher (64.24%). The high percentage of these two bacterial species shows that they are at the same time the most common bacterial pathogens causing pneumonia in pigs. The incidence of other species of bacteria isolated from the lungs of diseased pigs ranged from 0.51 to 10.88%. The sensitivity of isolated strains of Actinobacillus pleuropneumoniae to the selected range of antibiotics used in clinical veterinary medicine (penicillin, ampicillin, amoxicillin, cephalosporin (III gen.) gentamycin, streptomycin, neomycin, thylosine, enrofloxacin, linkomycin-spectinomycin, tetracycline, florphenycol, trimethoprimsulphomethoxazol and tulatromycin) was tested by the disc-diffusion method, with the implementation of antibiogram tabletes (Torlak) and antibiogram discs (Oxoid), on chocolate agar and on Chaemophilus test medium (HTM, Biomedics). All tested strains of Actinobacillus pleuropneumoniae were sensitive to thulatromycin, while resistance of same strains was the highest to tetracycline (53%) and trimethoprim/sulphomethoxazole (56%).
AB  - U novije vreme, pleuropneumonija izazvana aktinobacilusom dominira u velikim aglomeracijama životinja i u nekim zemljama predstavlja jedan od problema koji značajno doprinosi smanjenju populacije svinja. Navedeni problem, postoji i na našim farmama svinja gde protiče kao infekcija izazvana jednim uzročnikom, ili se, što je češća pojava, uzročnik javlja kao oportunistična patogena bakterija udružena sa drugim mikroorganizmima koji učestvuju u nastanku kompleksa respiratornih bolesti. Iz navedenih razloga monitoring zdravstvenog stanja jedinki na farmi je neophodan ne samo zbog utvrđivanja odgovarajuće strategije za kontrolu pleuropneumonije koju izaziva aktinobacilus, već i radi sprečavanja ogromnih ekonomskih gubitaka koje ova bolest može da prouzrokuje. U svim slučajevima sumnje na pleuropneumoniju kod svinja sa nekoliko farmi koje su bile obuhvaćene ispitivanjem, ustanovljeno je prisustvo Actinobacillus pleuropneumoniae. Podjednaka zastupljenost pleuropneumonije ustanovljena je, kako kod prasadi u odgoju, tako i kod prasadi u tovu. Od 237 uzoraka promenjenih delova pluća uginulih svinja iz 193 (81%) izolovano je 13 vrsta bakterija, a u okviru navedenog procenta zastupljenost Actinobacillus pleuropneumoniae je iznosila 33,15%. Morfološke, fiziološke i biohemijske osobine izolovanih sojeva Actinobacillus pleuropneumoniae potpuno su bile identične sa osobinama referentnog soja. Utvrđeno je da Actinobacillus pleuropneumoniae najobilnije raste na čokoladnom agaru kada se kao supstrat za obogaćenje koristi PolyVitex (bioMerieux). Od bakterija koje su iz pluća izolovane u čistoj kulturi najzastupljenije su bile Pasteurella multocida sa 32,64% i Actinobacillus pleuropneumoniae sa 29,01%. Njihovo zajedničko učešće u ukupno pozitivnim nalazima iznosi 61,65%, a ako se tome doda i njihovo učešće u mešanim infekcijama od 2,59 % onda taj procenat iznosi 64,24%. Ovako visoki procenti zastupljenosti obe vrste bakterija ukazuju na njihovu značajnu ulogu u nastanku pneumonije kod svinja. Zastupljenost ostalih vrsta bakterija izolovanih iz pluća obolelih svinja kretala se od 0,51% do 10,88%. Kod izolovanih sojeva Actinobacillus pleuropneumoniae osetljivost na odabrani broj antibiotika koji se koriste u kliničkoj praksi (penicilin, ampicilin, amoksicilin, cefalosporine (III gen.), gentamicin, streptomicin, neomicin, tilozin, enrofloksacin, linkomicin-spektinomicin, tetraciklin, florfenikol, trimetoprim-sulfometoksazol i tulatromicin) ispitivana je disk-difuzionom metodom, primenom antibiogram tableta (Torlak) i antibiogram diskova (Oxoid), na čokoladnom agaru i na hemofilus test medijumu (HTM, Biomedics). Svi ispitivani sojevi Actinobacillus pleuropneumoniae bili su osetljivi na tulatromicin, a najveća rezistencija kod istih sojeva, ustanovljena je na trimetoprim/sulfometoksazol od 56% i na tetraciklin od 53%.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Isolation and identification of Actinobacillus pleuropneumoniae in pig's lungs at farms and their sensitivity to antibiotics
T1  - Izolacija i identifikacija Actinobacillus pleuropneumoniae iz pluća svinja u farmskim uslovima držanja i njihova osetljivost na antibiotike
VL  - 58
IS  - 5-6
SP  - 499
EP  - 507
DO  - 10.2298/AVB0806499Z
ER  - 

@article{
author = "Žutić, Milenko and Ašanin, Ružica and Milić, Nenad and Ivetić, V. and Vidić, Branka and Žutić, Jadranka and Ašanin, Jelena",
year = "2008",
abstract = "The presence of Actinobacillus pleuropneumoniae has been established in all suspected cases of pleuropneumonia at several farms that have been included in the research. Equal incidence of pleuropneumonia has been found both among piglets for breeding and for fattening. The health monitoring of herds is extremely important, firstly because of the need for the adequate strategy to be chosen for controlling the Actinobacillus-caused pleuropneumonia and, at the same time, in order to prevent enormous economic losses that this disease may cause. The morphological, physiological and biochemical characteristics of isolated strains of Actinobacillus pleuropneumoniae were completely identical to those of referent strains. It has been found that Actinobacillus pleuropneumoniae grows most abundantly on chocolate agar when PolyVitex (bioMerieux) is used as the substrate. Out of 237 samples of altered parts of swine lungs, 13 bacterial species have been found in 193 (81%), and the incidence of Actinobacillus pleuropneumoniae within this percentage was 33%. Of all bacterial species isolated in pure culture from all investigated specimen the most dominant species were Pasteurella multocida with the incidence of 32.64% and Actinobacillus pleuropneumoniae with 29.01%. Their common incidence in all positive findings was 61.65%. If one adds to this their participation in mixed infections (2.59%), this percentage is higher (64.24%). The high percentage of these two bacterial species shows that they are at the same time the most common bacterial pathogens causing pneumonia in pigs. The incidence of other species of bacteria isolated from the lungs of diseased pigs ranged from 0.51 to 10.88%. The sensitivity of isolated strains of Actinobacillus pleuropneumoniae to the selected range of antibiotics used in clinical veterinary medicine (penicillin, ampicillin, amoxicillin, cephalosporin (III gen.) gentamycin, streptomycin, neomycin, thylosine, enrofloxacin, linkomycin-spectinomycin, tetracycline, florphenycol, trimethoprimsulphomethoxazol and tulatromycin) was tested by the disc-diffusion method, with the implementation of antibiogram tabletes (Torlak) and antibiogram discs (Oxoid), on chocolate agar and on Chaemophilus test medium (HTM, Biomedics). All tested strains of Actinobacillus pleuropneumoniae were sensitive to thulatromycin, while resistance of same strains was the highest to tetracycline (53%) and trimethoprim/sulphomethoxazole (56%)., U novije vreme, pleuropneumonija izazvana aktinobacilusom dominira u velikim aglomeracijama životinja i u nekim zemljama predstavlja jedan od problema koji značajno doprinosi smanjenju populacije svinja. Navedeni problem, postoji i na našim farmama svinja gde protiče kao infekcija izazvana jednim uzročnikom, ili se, što je češća pojava, uzročnik javlja kao oportunistična patogena bakterija udružena sa drugim mikroorganizmima koji učestvuju u nastanku kompleksa respiratornih bolesti. Iz navedenih razloga monitoring zdravstvenog stanja jedinki na farmi je neophodan ne samo zbog utvrđivanja odgovarajuće strategije za kontrolu pleuropneumonije koju izaziva aktinobacilus, već i radi sprečavanja ogromnih ekonomskih gubitaka koje ova bolest može da prouzrokuje. U svim slučajevima sumnje na pleuropneumoniju kod svinja sa nekoliko farmi koje su bile obuhvaćene ispitivanjem, ustanovljeno je prisustvo Actinobacillus pleuropneumoniae. Podjednaka zastupljenost pleuropneumonije ustanovljena je, kako kod prasadi u odgoju, tako i kod prasadi u tovu. Od 237 uzoraka promenjenih delova pluća uginulih svinja iz 193 (81%) izolovano je 13 vrsta bakterija, a u okviru navedenog procenta zastupljenost Actinobacillus pleuropneumoniae je iznosila 33,15%. Morfološke, fiziološke i biohemijske osobine izolovanih sojeva Actinobacillus pleuropneumoniae potpuno su bile identične sa osobinama referentnog soja. Utvrđeno je da Actinobacillus pleuropneumoniae najobilnije raste na čokoladnom agaru kada se kao supstrat za obogaćenje koristi PolyVitex (bioMerieux). Od bakterija koje su iz pluća izolovane u čistoj kulturi najzastupljenije su bile Pasteurella multocida sa 32,64% i Actinobacillus pleuropneumoniae sa 29,01%. Njihovo zajedničko učešće u ukupno pozitivnim nalazima iznosi 61,65%, a ako se tome doda i njihovo učešće u mešanim infekcijama od 2,59 % onda taj procenat iznosi 64,24%. Ovako visoki procenti zastupljenosti obe vrste bakterija ukazuju na njihovu značajnu ulogu u nastanku pneumonije kod svinja. Zastupljenost ostalih vrsta bakterija izolovanih iz pluća obolelih svinja kretala se od 0,51% do 10,88%. Kod izolovanih sojeva Actinobacillus pleuropneumoniae osetljivost na odabrani broj antibiotika koji se koriste u kliničkoj praksi (penicilin, ampicilin, amoksicilin, cefalosporine (III gen.), gentamicin, streptomicin, neomicin, tilozin, enrofloksacin, linkomicin-spektinomicin, tetraciklin, florfenikol, trimetoprim-sulfometoksazol i tulatromicin) ispitivana je disk-difuzionom metodom, primenom antibiogram tableta (Torlak) i antibiogram diskova (Oxoid), na čokoladnom agaru i na hemofilus test medijumu (HTM, Biomedics). Svi ispitivani sojevi Actinobacillus pleuropneumoniae bili su osetljivi na tulatromicin, a najveća rezistencija kod istih sojeva, ustanovljena je na trimetoprim/sulfometoksazol od 56% i na tetraciklin od 53%.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Isolation and identification of Actinobacillus pleuropneumoniae in pig's lungs at farms and their sensitivity to antibiotics, Izolacija i identifikacija Actinobacillus pleuropneumoniae iz pluća svinja u farmskim uslovima držanja i njihova osetljivost na antibiotike",
volume = "58",
number = "5-6",
pages = "499-507",
doi = "10.2298/AVB0806499Z"
}

Žutić, M., Ašanin, R., Milić, N., Ivetić, V., Vidić, B., Žutić, J.,& Ašanin, J.. (2008). Isolation and identification of Actinobacillus pleuropneumoniae in pig's lungs at farms and their sensitivity to antibiotics. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(5-6), 499-507.
https://doi.org/10.2298/AVB0806499Z

Žutić M, Ašanin R, Milić N, Ivetić V, Vidić B, Žutić J, Ašanin J. Isolation and identification of Actinobacillus pleuropneumoniae in pig's lungs at farms and their sensitivity to antibiotics. in Acta Veterinaria-Beograd. 2008;58(5-6):499-507.
doi:10.2298/AVB0806499Z .

Žutić, Milenko, Ašanin, Ružica, Milić, Nenad, Ivetić, V., Vidić, Branka, Žutić, Jadranka, Ašanin, Jelena, "Isolation and identification of Actinobacillus pleuropneumoniae in pig's lungs at farms and their sensitivity to antibiotics" in Acta Veterinaria-Beograd, 58, no. 5-6 (2008):499-507,
https://doi.org/10.2298/AVB0806499Z . .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Žižović, Irena
AU  - Stamenić, Marko
AU  - Ašanin, Ružica
AU  - Ristić, Mihailo
AU  - Petrović, Slobodan D.
AU  - Skala, Dejan
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/544
PB  - Elsevier Science Sa, Lausanne
T2  - Biochemical Engineering Journal
T1  - Antimicrobial activity of celery fruit isolates and SFE process modeling
VL  - 42
IS  - 2
SP  - 148
EP  - 152
DO  - 10.1016/j.bej.2008.06.008
ER  - 

@article{
author = "Mišić, Dušan and Žižović, Irena and Stamenić, Marko and Ašanin, Ružica and Ristić, Mihailo and Petrović, Slobodan D. and Skala, Dejan",
year = "2008",
publisher = "Elsevier Science Sa, Lausanne",
journal = "Biochemical Engineering Journal",
title = "Antimicrobial activity of celery fruit isolates and SFE process modeling",
volume = "42",
number = "2",
pages = "148-152",
doi = "10.1016/j.bej.2008.06.008"
}

Mišić, D., Žižović, I., Stamenić, M., Ašanin, R., Ristić, M., Petrović, S. D.,& Skala, D.. (2008). Antimicrobial activity of celery fruit isolates and SFE process modeling. in Biochemical Engineering Journal
Elsevier Science Sa, Lausanne., 42(2), 148-152.
https://doi.org/10.1016/j.bej.2008.06.008

Mišić D, Žižović I, Stamenić M, Ašanin R, Ristić M, Petrović SD, Skala D. Antimicrobial activity of celery fruit isolates and SFE process modeling. in Biochemical Engineering Journal. 2008;42(2):148-152.
doi:10.1016/j.bej.2008.06.008 .

Mišić, Dušan, Žižović, Irena, Stamenić, Marko, Ašanin, Ružica, Ristić, Mihailo, Petrović, Slobodan D., Skala, Dejan, "Antimicrobial activity of celery fruit isolates and SFE process modeling" in Biochemical Engineering Journal, 42, no. 2 (2008):148-152,
https://doi.org/10.1016/j.bej.2008.06.008 . .

TY  - JOUR
AU  - Marković, Ljiljana
AU  - Ašanin, Ružica
AU  - Radojičić, Sonja
AU  - Isenović, Esma
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/491
AB  - Monoclonal antibodies (MAbs) against Pseudorabies virus (PrV) were obtained by the fusion of P3x-Ag8.653 myeloma and spleen cells from immunized BALB/c mice with a suspension of Pseudorabies (PrV) virus strains: MAVE (Morbus Aujeszk'y virus Ercegovac) and NS 257 (Novosadski virus strain). A total of 95 antibody-secreting hybridoma cells against the virus strain (MAVE and NS 257) of Pseudorabies virus have been isolated. Ten of these monoclonal antibodies were found by ELISA (Enzyme-linked immunosorbent assay) to react specifically with both virus strains. MAbs for VAM 2.1, VAM 4.1, VAM 5.1 and VAM 6.1 were purified by chromatography on protein G Sepharose 4FF and they have been shown to have a strong reactivity in the ELISA test. All MAbs were characterized by electrophoresis SDS-PAGE and electrophoresis Western-blot immunoassay. MAbs VAM 2.1, VAM 4.1, VAM 5.1 and VAM 6.1 in hybridoma culture supernatants and ascites fluid were quantified using a dot-blot immunobinding assay. The VAM 2.1 MAb was found to be more specific in the reaction with viruses of both strains. The glycoprotein of 40 kD molecular weight was found on the surface of virus strain MAVE. Results showed that the produced and characterized MAbs against PrV strains can be used for the detection of Aujeszky's disease.
AB  - Monoklonska antitela (MAbs) specifična za Pseudorabies virus (PrV) dobijena su fuzijom ćelija mijelocita P3x-Ag8.653 i splenocita miševa BALB/c koji su imunizovani suspenzijama sojeva MAVE (Morbus Aujeszk'y virus Ercegovac) i NS 257 (soj 257 Novi Sad). U 95 uzoraka supernatanata hibridnih ćelija primenom ELISA metode ispitivano je prisustvo antitela specifičnih za sojeve virusa (MAVE i NS 257). Navedenom metodom otkriveno je deset monoklonskih antitela koja su specifično reagovala sa oba soja virusa. Izdvojena četiri monoklonska antitela označena kao VAM2.1, VAM4.1, VAM5.1 i VAM 6.1, radi dalje analize, prečišćena su metodom hromatografije na koloni protein G Sepharosa 4FF, a zatim ponovo ispitana ELISA metodom. Karakterizacija izdvojenih MAbs za navedene viruse urađena je metodama elektroforeze SDS-PAGE i Westernblott. Dot-blott metodom iz supernatanata hibridnih ćelija i ascitne tečnosti kvantifikovana su monoklonska antitela VAM 2.1, VAM 4.1, VAM 5.1 i VAM 6.1. Monoklonsko antitelo VAM 2.1 pokazalo je visok nivo aktivnosti u reakciji sa oba soja virusa. Analizom proteinskog profila virusnog omotača soja MAVE detektovan je glikoprotein molekulske mase 40 kD. Ova ispitivanja su ukazala da monoklonska antitela specifična za sojeve PrV mogu da se primene u otkrivanju Aujeskijeve bolesti.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Production and characterization of monoclonal antibodies specific for Pseudorabies virus
T1  - Dobijanje i karakterizacija monoklonskih antitela specifičnih za Pseudorabies virus
VL  - 57
IS  - 5-6
SP  - 441
EP  - 451
DO  - 10.2298/AVB0706441M
ER  - 

@article{
author = "Marković, Ljiljana and Ašanin, Ružica and Radojičić, Sonja and Isenović, Esma",
year = "2007",
abstract = "Monoclonal antibodies (MAbs) against Pseudorabies virus (PrV) were obtained by the fusion of P3x-Ag8.653 myeloma and spleen cells from immunized BALB/c mice with a suspension of Pseudorabies (PrV) virus strains: MAVE (Morbus Aujeszk'y virus Ercegovac) and NS 257 (Novosadski virus strain). A total of 95 antibody-secreting hybridoma cells against the virus strain (MAVE and NS 257) of Pseudorabies virus have been isolated. Ten of these monoclonal antibodies were found by ELISA (Enzyme-linked immunosorbent assay) to react specifically with both virus strains. MAbs for VAM 2.1, VAM 4.1, VAM 5.1 and VAM 6.1 were purified by chromatography on protein G Sepharose 4FF and they have been shown to have a strong reactivity in the ELISA test. All MAbs were characterized by electrophoresis SDS-PAGE and electrophoresis Western-blot immunoassay. MAbs VAM 2.1, VAM 4.1, VAM 5.1 and VAM 6.1 in hybridoma culture supernatants and ascites fluid were quantified using a dot-blot immunobinding assay. The VAM 2.1 MAb was found to be more specific in the reaction with viruses of both strains. The glycoprotein of 40 kD molecular weight was found on the surface of virus strain MAVE. Results showed that the produced and characterized MAbs against PrV strains can be used for the detection of Aujeszky's disease., Monoklonska antitela (MAbs) specifična za Pseudorabies virus (PrV) dobijena su fuzijom ćelija mijelocita P3x-Ag8.653 i splenocita miševa BALB/c koji su imunizovani suspenzijama sojeva MAVE (Morbus Aujeszk'y virus Ercegovac) i NS 257 (soj 257 Novi Sad). U 95 uzoraka supernatanata hibridnih ćelija primenom ELISA metode ispitivano je prisustvo antitela specifičnih za sojeve virusa (MAVE i NS 257). Navedenom metodom otkriveno je deset monoklonskih antitela koja su specifično reagovala sa oba soja virusa. Izdvojena četiri monoklonska antitela označena kao VAM2.1, VAM4.1, VAM5.1 i VAM 6.1, radi dalje analize, prečišćena su metodom hromatografije na koloni protein G Sepharosa 4FF, a zatim ponovo ispitana ELISA metodom. Karakterizacija izdvojenih MAbs za navedene viruse urađena je metodama elektroforeze SDS-PAGE i Westernblott. Dot-blott metodom iz supernatanata hibridnih ćelija i ascitne tečnosti kvantifikovana su monoklonska antitela VAM 2.1, VAM 4.1, VAM 5.1 i VAM 6.1. Monoklonsko antitelo VAM 2.1 pokazalo je visok nivo aktivnosti u reakciji sa oba soja virusa. Analizom proteinskog profila virusnog omotača soja MAVE detektovan je glikoprotein molekulske mase 40 kD. Ova ispitivanja su ukazala da monoklonska antitela specifična za sojeve PrV mogu da se primene u otkrivanju Aujeskijeve bolesti.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Production and characterization of monoclonal antibodies specific for Pseudorabies virus, Dobijanje i karakterizacija monoklonskih antitela specifičnih za Pseudorabies virus",
volume = "57",
number = "5-6",
pages = "441-451",
doi = "10.2298/AVB0706441M"
}

Marković, L., Ašanin, R., Radojičić, S.,& Isenović, E.. (2007). Production and characterization of monoclonal antibodies specific for Pseudorabies virus. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(5-6), 441-451.
https://doi.org/10.2298/AVB0706441M

Marković L, Ašanin R, Radojičić S, Isenović E. Production and characterization of monoclonal antibodies specific for Pseudorabies virus. in Acta Veterinaria-Beograd. 2007;57(5-6):441-451.
doi:10.2298/AVB0706441M .

Marković, Ljiljana, Ašanin, Ružica, Radojičić, Sonja, Isenović, Esma, "Production and characterization of monoclonal antibodies specific for Pseudorabies virus" in Acta Veterinaria-Beograd, 57, no. 5-6 (2007):441-451,
https://doi.org/10.2298/AVB0706441M . .

TY  - JOUR
AU  - Filipović, Irina
AU  - Mišić, Dušan
AU  - Ašanin, Ružica
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/464
AB  - Bacterial strains which possess genes to produce ESBL most often are multiresistant and also carry genes responsible for the resistance to most other antibiotics, including aminoglycosides, sulfamethoxazole+trimethoprim and fluoroquinolones. Therefore, practically the biggest contemporary clinical problem are infections of humans and animals caused by ESBL-producing strains of E. coli, Kleibsiella, Enterobacter, Proteus, Serratia, Citrobacter, Salmonella and Shigella species. The investigation of the ESBL presence was completed on multiresistant E. coli and Salmonella strains originating from dogs, cats, cattle, sheep, goats, pigs and poultry. The investigated strains were isolated from ear, skin, vaginal, faecal, urine, egg and eggshell swabs, from healthy and diseased individual animals of various ages and breed categories. The sum of 112 E. coli and 45 Salmonella strains was investigated. All strains resistant to 3 or more antibiotics were categorized as multiresistant, which led to a conclusion that 35 E. coli and 6 Salmonella strains out of all investigated were multiresistant to antibiotics. The largest number of multiresistant E. coli strains was discovered in cattle - 12 in total, and the minimal number in goats and sheep, with two strains each. All multiresistant Salmonella strains belonged to the Salmonella Enteritidis species (S. enterica subsp. enterica serovar Enteritidis). The sum of multiresistant Salmonella strains compared to all investigated strains was relatively low (13.3%), but the resistance prevalence for some antibiotics in these strains was extremely high, for ampicillin and amoxicillin with clavulanic acid as high as 100%, and for tetracycline 83.3%. For the control in this investigation were used ESBL positive E. coli strains originated in human urine specimens. No presence of positive ESBL strains was established. However, when the screening investigation was performed, almost all the strains were suspect, thus a confirmatory test had to be performed for all strains.
AB  - Sojevi bakterija koji poseduju gene za proizvodnju beta-laktamaza proširenog spektra delovanja (ESBL) najčešće ispoljavaju multirezistenciju i istovremeno su nosioci gena odgovornih za rezistenciju na većinu drugih antibiotika, uključujući i aminoglikozide, sulfametoksazol+trimetoprim i fluorohinolone. Zbog navedenih razloga, najveći problem danas u kliničkoj praksi predstavljaju infekcije ljudi i životinja izazvane ESBL produkujućim sojevima E.coli, Klebsiella, Enterobacter, Proteus, Serratia, Citrobacter, Salmonella i Shigella vrsta. U ovim ispitivanjima materijali za izolovanje sojeva bakterija predstavljali su brisevi ušiju, kože, vaginalni brisevi, feces i urin, jaja i ljuske od jaja. Primenom klasičnih i komercijalnih testova mikrobiološke dijagnostike izolovani su i identifikovani sojevi E.coli i Salmonella vrsta. Navedenim sojevima je ispitivana i osetljivost na određeni broj antibiotika i hemioterapeutika primenom metoda propisanih od strane CLSI, USA iz 2006. Samo oni sojevi E.coli i Salmonella poreklom od pasa, mačaka, goveda, ovaca, koza, svinja i živine kod kojih je ustanovljena rezistencija na 3 i više antibiotika kategorizovani su kao multirezistentni i dalje su ispitivani na prisustvo betalaktamaza proširenog spektra delovanja. Ovim ispitivanjem obuhvaćeno je ukupno 112 sojeva E.coli i 45 sojeva Salmonella. Takođe su ispitivanjem bila obuhvać ena i dva ESBL pozitivna soja E.coli koji su izolovani u našoj laboratoriji iz urina ljudi, a služili su nam kao kontrola. Od 112 ispitivanih sojeva E.coli, kod 35 je utvrđena multirezistencija na određeni broj antibiotika, dok je od 45 sojeva Salmonella koji su ispitivani samo kod 6 sojeva ustanovljena multirezistencija. Najveći broj multirezistetnih sojeva E.coli (MR E.coli) otkriven je kod goveda, ukupno 12, a najmanje kod koza i ovaca, po dva soja. Svi multirezistentni sojevi Salmonella pripadali su vrsti Salmonella enteritidis (S.enterica subsp. enterica serovar. enteritidis). Ukupan broj multirezistentnih sojeva Salmonella u odnosu na broj sojeva koji su ispitivani bio je relativno nizak (13,3 %). Međutim, izuzetno visoka prevalencija rezistencije kod ispitivanih sojeva utvrđena je na ampicilin i amoksicilin sa klavulanskom kiselinom (100 %), a na tetraciklin 83,3%. Primenom "screening" ispitivanja, gotovo svi sojevi uključeni u ispitivanje bili su sumnjivi na prisustvo ESBL i zbog toga je u ispitivanje bio uključen i potvrdni test. Tek po završenim ispitivanjima, mogao se izvesti zaključak da među izolovanim sojevima E.coli i Salmonella poreklom od životinja nisu otkriveni sojevi koji poseduju _ - laktamaze proširenog spektra delovanja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Investigation of the presence of extended spectrum beta-lactamases (ESBL) in multiresistant strains of E. Coli and salmonella species originated from domestic animals
T1  - Ispitivanje prisustva beta-laktamaza proširenog spektra delovanja (ESBL) kod multirezistentnih sojeva E.Coli i salmonella vrsta poreklom od domaćih životinja
VL  - 57
IS  - 4
SP  - 369
EP  - 379
DO  - 10.2298/AVB0704369F
ER  - 

@article{
author = "Filipović, Irina and Mišić, Dušan and Ašanin, Ružica",
year = "2007",
abstract = "Bacterial strains which possess genes to produce ESBL most often are multiresistant and also carry genes responsible for the resistance to most other antibiotics, including aminoglycosides, sulfamethoxazole+trimethoprim and fluoroquinolones. Therefore, practically the biggest contemporary clinical problem are infections of humans and animals caused by ESBL-producing strains of E. coli, Kleibsiella, Enterobacter, Proteus, Serratia, Citrobacter, Salmonella and Shigella species. The investigation of the ESBL presence was completed on multiresistant E. coli and Salmonella strains originating from dogs, cats, cattle, sheep, goats, pigs and poultry. The investigated strains were isolated from ear, skin, vaginal, faecal, urine, egg and eggshell swabs, from healthy and diseased individual animals of various ages and breed categories. The sum of 112 E. coli and 45 Salmonella strains was investigated. All strains resistant to 3 or more antibiotics were categorized as multiresistant, which led to a conclusion that 35 E. coli and 6 Salmonella strains out of all investigated were multiresistant to antibiotics. The largest number of multiresistant E. coli strains was discovered in cattle - 12 in total, and the minimal number in goats and sheep, with two strains each. All multiresistant Salmonella strains belonged to the Salmonella Enteritidis species (S. enterica subsp. enterica serovar Enteritidis). The sum of multiresistant Salmonella strains compared to all investigated strains was relatively low (13.3%), but the resistance prevalence for some antibiotics in these strains was extremely high, for ampicillin and amoxicillin with clavulanic acid as high as 100%, and for tetracycline 83.3%. For the control in this investigation were used ESBL positive E. coli strains originated in human urine specimens. No presence of positive ESBL strains was established. However, when the screening investigation was performed, almost all the strains were suspect, thus a confirmatory test had to be performed for all strains., Sojevi bakterija koji poseduju gene za proizvodnju beta-laktamaza proširenog spektra delovanja (ESBL) najčešće ispoljavaju multirezistenciju i istovremeno su nosioci gena odgovornih za rezistenciju na većinu drugih antibiotika, uključujući i aminoglikozide, sulfametoksazol+trimetoprim i fluorohinolone. Zbog navedenih razloga, najveći problem danas u kliničkoj praksi predstavljaju infekcije ljudi i životinja izazvane ESBL produkujućim sojevima E.coli, Klebsiella, Enterobacter, Proteus, Serratia, Citrobacter, Salmonella i Shigella vrsta. U ovim ispitivanjima materijali za izolovanje sojeva bakterija predstavljali su brisevi ušiju, kože, vaginalni brisevi, feces i urin, jaja i ljuske od jaja. Primenom klasičnih i komercijalnih testova mikrobiološke dijagnostike izolovani su i identifikovani sojevi E.coli i Salmonella vrsta. Navedenim sojevima je ispitivana i osetljivost na određeni broj antibiotika i hemioterapeutika primenom metoda propisanih od strane CLSI, USA iz 2006. Samo oni sojevi E.coli i Salmonella poreklom od pasa, mačaka, goveda, ovaca, koza, svinja i živine kod kojih je ustanovljena rezistencija na 3 i više antibiotika kategorizovani su kao multirezistentni i dalje su ispitivani na prisustvo betalaktamaza proširenog spektra delovanja. Ovim ispitivanjem obuhvaćeno je ukupno 112 sojeva E.coli i 45 sojeva Salmonella. Takođe su ispitivanjem bila obuhvać ena i dva ESBL pozitivna soja E.coli koji su izolovani u našoj laboratoriji iz urina ljudi, a služili su nam kao kontrola. Od 112 ispitivanih sojeva E.coli, kod 35 je utvrđena multirezistencija na određeni broj antibiotika, dok je od 45 sojeva Salmonella koji su ispitivani samo kod 6 sojeva ustanovljena multirezistencija. Najveći broj multirezistetnih sojeva E.coli (MR E.coli) otkriven je kod goveda, ukupno 12, a najmanje kod koza i ovaca, po dva soja. Svi multirezistentni sojevi Salmonella pripadali su vrsti Salmonella enteritidis (S.enterica subsp. enterica serovar. enteritidis). Ukupan broj multirezistentnih sojeva Salmonella u odnosu na broj sojeva koji su ispitivani bio je relativno nizak (13,3 %). Međutim, izuzetno visoka prevalencija rezistencije kod ispitivanih sojeva utvrđena je na ampicilin i amoksicilin sa klavulanskom kiselinom (100 %), a na tetraciklin 83,3%. Primenom "screening" ispitivanja, gotovo svi sojevi uključeni u ispitivanje bili su sumnjivi na prisustvo ESBL i zbog toga je u ispitivanje bio uključen i potvrdni test. Tek po završenim ispitivanjima, mogao se izvesti zaključak da među izolovanim sojevima E.coli i Salmonella poreklom od životinja nisu otkriveni sojevi koji poseduju _ - laktamaze proširenog spektra delovanja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Investigation of the presence of extended spectrum beta-lactamases (ESBL) in multiresistant strains of E. Coli and salmonella species originated from domestic animals, Ispitivanje prisustva beta-laktamaza proširenog spektra delovanja (ESBL) kod multirezistentnih sojeva E.Coli i salmonella vrsta poreklom od domaćih životinja",
volume = "57",
number = "4",
pages = "369-379",
doi = "10.2298/AVB0704369F"
}

Filipović, I., Mišić, D.,& Ašanin, R.. (2007). Investigation of the presence of extended spectrum beta-lactamases (ESBL) in multiresistant strains of E. Coli and salmonella species originated from domestic animals. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(4), 369-379.
https://doi.org/10.2298/AVB0704369F

Filipović I, Mišić D, Ašanin R. Investigation of the presence of extended spectrum beta-lactamases (ESBL) in multiresistant strains of E. Coli and salmonella species originated from domestic animals. in Acta Veterinaria-Beograd. 2007;57(4):369-379.
doi:10.2298/AVB0704369F .

Filipović, Irina, Mišić, Dušan, Ašanin, Ružica, "Investigation of the presence of extended spectrum beta-lactamases (ESBL) in multiresistant strains of E. Coli and salmonella species originated from domestic animals" in Acta Veterinaria-Beograd, 57, no. 4 (2007):369-379,
https://doi.org/10.2298/AVB0704369F . .

TY  - JOUR
AU  - Milanov, Dubravka
AU  - Ašanin, Ružica
AU  - Mišić, Dušan
AU  - Vidić, Branka
AU  - Ratajac, Radomir
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/461
AB  - Listeria monocytogenes is the causative agent of listeriosis in humans and animals and an important food-born pathogen. Control of its presence in food processing plants, particularly on sites where food contamination is expected, is of paramount importance with respect to food safety and protection of human health. Numerous studies demonstrated that this organism can be isolated during several months, even several years, from diverse sites in food processing plants, which is due to its ability to adsorb onto inert surfaces and form a biofilm, alone or in coexistence with other bacterial species. In this study we investigated the ability of 16 animal isolates of L. monocytogenes to form a biofilm on polystyrene microtiter plates. The investigation was performed at three different temperatures 4oC, 25 oC and 37oC that are commonly suitable for growth of Listeria monocytogenes. The research was carried out using three different nutritive media: trypthon-soy broth with yeast extract (TSB-YE), brainheart infusion (BHI) and 1/20 diluted trypthon-soy broth with yeast extract (1/20 TSB-YE). In order to investigate the biofilm formation in vitro an inoculum was prepared from 24-hours-old cultures of isolated strains of L.monocytogenes. The density of the inoculum was 2-10x107 cfu/mL (OD600=0.093 ± 0.009 in TSB-YE). The microtiter plates were incubated at cited temperatures during 48h. Colonization rate of L.monocytogenes strains on polystyrene surface and biofilm formation were monitored using crystal violet stain added to the microtiter plates, as well as using light microscopy. The tested strains demonstrated a diverse ability of biofilm formation, depending on the incubation temperature and nutritive medium. In this paper we presented the results obtained for four strains of Listeria monocytogenes isolated from brain samples of sheep, designated as 785/05, 593/05, 748/05, 1915/04 and one strain isolated from aborted calve's fetus, designated as 021/04. The referent strain of L.monocytogenes, 1071 (4b), was used as a control. Strains of L. monocytogenes cultured in nutrient-rich media (e.g. TSB-YE) and at higher temperatures (37oC) exhibited a higher ability of biofilm formation. However, non of the studied strains could be classified as a good biofilm producer, disregard of the incubation temperature and medium used. The highest OD values were obtained at the incubation temperature of 37oC in TSB-YE (OD595=0.346 ± 0.055), when three strains were quantified as moderate and two as weak biofilm producers. The decrease of incubation temperature resulted in decreased OD values, thus four strains were classified as weak biofilm producers at 25oC (OD595=0.289 ± 0.083), and none of the investigated strains was assessed as biofilm producers at 4oC (OD595=0.124 ± 0.011) Light-microscopy examination, confirmed by quantitative values obtained by the crystal violet microtiter test, proved to be a simple and rapid screening method for the quantification of the ability of L.monocytogenes to form biofilms in varying test conditions.
AB  - Listeria monocytogenes je uzročnik listerioze ljudi i životinja i veoma važan patogen koji pored ostalih načina prenošenja može da se prenese i hranom. Kon- trola njenog prisustva u pogonima prehrambene industrije, naročito na mestima moguće kontaminacije namirnica, od izuzetnog je značaja u proizvodnji zdravstveno bezbedne hrane i zaštiti zdravlja ljudi. Istraživanja su pokazala da se ova bakterija više meseci ili godina uzastopno može izolovati sa različitih mesta sa kojima u kontakt dolaze namirnice, upravo zahvaljujući sposobnosti da se vezuje za inertne površine i formira biofilm, sama ili u zajednici sa drugim vrstama bakterija. U ovom radu je ispitivana sposobnost formiranja biofilma u mikrotitracionim pločama od polistirena kod 16 izolovanih sojeva L. monocytogenes, poreklom od životinja. Ispitivanja su vršena na temperaturama od 4oC, 25oC i 37oC, na kojima Listeria monocytogenes može uobičajeno da raste. Za navedena ispitivanja korišč ene su tri hranljive podloge različitog sastava, kao što su tripton soja bujon sa kvaščevim ekstraktom (TSB-YE), moždano srčana infuzija (BHI) i tripton soja bujon sa kvaščevim ekstraktom razređen u odnosu 1:20 fiziološkim rastvorom (1/20 TSB-YE). Za ispitivanja formiranja biofilma in vitro pripreman je inokulum od kultura izolovanih sojeva L. monocytogenes starih 24 časa. Gustina pripremljenog inokuluma iznosila je 2-10x107 CFU/ml (OD600=0,093 ± 0,009 u TSB-YE). Mikrotitracione ploče nakon inokulisanja inkubisane su tokom 48 časova na prethodno navedenim temperaturama. Kolonizacija sojeva L. monocytogenes na polistiren i formiranje biofilma, praćeno je pomoću kristal violet boje koja je dodavana u mikrotitracione ploče, kao i pomoću svetlosnog mikroskopa. Sojevi Listeria monocytogenes koji su ispitivani pokazali su različitu sposobnost formiranja biofilma u zavisnosti od temperature inkubacije i podloge koja je korišćena za rast. Zbog navedenih činjenica u radu su prikazani rezultati dobijeni ispitivanjem četiri soja Listeria monocytogenes koji su izolovani iz uzoraka mozga obolelih ovaca i označeni brojevima 785/05, 593/05, 748/05, 1915/04 i jedan soj izolovan iz pobač enog fetusa krave označen brojem 021/04, kao i jednog referentnog soja L. monocytogenes 1071 (4b) koji je korišćen kao kontrola. Sojevi L. monocytogenes koji su bili zasejavani u podloge bogatije hranljivim materijama, kao što je (TSBYE) i kultivisani na višim temperaturama (na temperaturi od 37 oC) bolje su formirali biofilm. Nijedan od ispitivanih sojeva nije mogao biti procenjen kao jak biofilm "producer", bez obzira na temperaturu inkubacije ili korišćenu podlogu. Najviše OD vrednosti dobijene su na temperaturi inkubacije od 37oC u TSB-YE (OD595=0,346 ±0,055), kada su tri ispitana soja kategorisana kao umereni, a dva kao slabi produktori biofilma. Sa smanjenjem temperature inkubacije u istoj podlozi, smanjivale su se i OD vrednosti, pa su na temperaturi od 25oC (OD595=0,289 ± 0,083) četiri soja procenjena kao slabi biofilm, a na temperaturi od 4oC (OD595=0,124 ± 0,011) nijedan soj nije procenjen kao produktor biofilma. Kvantitativne vrednosti dobijene primenom mikrotitracionog testa sa kristal violet bojom, koji se pokazao kao jednostavna i brza (skrining) metoda za procenu sposobnosti sojeva L. monocytogenes da formiraju biofilm pod različitim uslovima, potvrđene su i primenom svetlosne mikroskopije.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Investigation of biofilm formation in vitro ability of Listeria monocytogenes strains isolated from animals
T1  - Ispitivanje sposobnosti formiranja biofilma u uslovima in vitro sojeva Listeria monocytogenes izolovanih od životinja
VL  - 57
IS  - 5-6
SP  - 429
EP  - 440
DO  - 10.2298/AVB0706429M
ER  - 

@article{
author = "Milanov, Dubravka and Ašanin, Ružica and Mišić, Dušan and Vidić, Branka and Ratajac, Radomir",
year = "2007",
abstract = "Listeria monocytogenes is the causative agent of listeriosis in humans and animals and an important food-born pathogen. Control of its presence in food processing plants, particularly on sites where food contamination is expected, is of paramount importance with respect to food safety and protection of human health. Numerous studies demonstrated that this organism can be isolated during several months, even several years, from diverse sites in food processing plants, which is due to its ability to adsorb onto inert surfaces and form a biofilm, alone or in coexistence with other bacterial species. In this study we investigated the ability of 16 animal isolates of L. monocytogenes to form a biofilm on polystyrene microtiter plates. The investigation was performed at three different temperatures 4oC, 25 oC and 37oC that are commonly suitable for growth of Listeria monocytogenes. The research was carried out using three different nutritive media: trypthon-soy broth with yeast extract (TSB-YE), brainheart infusion (BHI) and 1/20 diluted trypthon-soy broth with yeast extract (1/20 TSB-YE). In order to investigate the biofilm formation in vitro an inoculum was prepared from 24-hours-old cultures of isolated strains of L.monocytogenes. The density of the inoculum was 2-10x107 cfu/mL (OD600=0.093 ± 0.009 in TSB-YE). The microtiter plates were incubated at cited temperatures during 48h. Colonization rate of L.monocytogenes strains on polystyrene surface and biofilm formation were monitored using crystal violet stain added to the microtiter plates, as well as using light microscopy. The tested strains demonstrated a diverse ability of biofilm formation, depending on the incubation temperature and nutritive medium. In this paper we presented the results obtained for four strains of Listeria monocytogenes isolated from brain samples of sheep, designated as 785/05, 593/05, 748/05, 1915/04 and one strain isolated from aborted calve's fetus, designated as 021/04. The referent strain of L.monocytogenes, 1071 (4b), was used as a control. Strains of L. monocytogenes cultured in nutrient-rich media (e.g. TSB-YE) and at higher temperatures (37oC) exhibited a higher ability of biofilm formation. However, non of the studied strains could be classified as a good biofilm producer, disregard of the incubation temperature and medium used. The highest OD values were obtained at the incubation temperature of 37oC in TSB-YE (OD595=0.346 ± 0.055), when three strains were quantified as moderate and two as weak biofilm producers. The decrease of incubation temperature resulted in decreased OD values, thus four strains were classified as weak biofilm producers at 25oC (OD595=0.289 ± 0.083), and none of the investigated strains was assessed as biofilm producers at 4oC (OD595=0.124 ± 0.011) Light-microscopy examination, confirmed by quantitative values obtained by the crystal violet microtiter test, proved to be a simple and rapid screening method for the quantification of the ability of L.monocytogenes to form biofilms in varying test conditions., Listeria monocytogenes je uzročnik listerioze ljudi i životinja i veoma važan patogen koji pored ostalih načina prenošenja može da se prenese i hranom. Kon- trola njenog prisustva u pogonima prehrambene industrije, naročito na mestima moguće kontaminacije namirnica, od izuzetnog je značaja u proizvodnji zdravstveno bezbedne hrane i zaštiti zdravlja ljudi. Istraživanja su pokazala da se ova bakterija više meseci ili godina uzastopno može izolovati sa različitih mesta sa kojima u kontakt dolaze namirnice, upravo zahvaljujući sposobnosti da se vezuje za inertne površine i formira biofilm, sama ili u zajednici sa drugim vrstama bakterija. U ovom radu je ispitivana sposobnost formiranja biofilma u mikrotitracionim pločama od polistirena kod 16 izolovanih sojeva L. monocytogenes, poreklom od životinja. Ispitivanja su vršena na temperaturama od 4oC, 25oC i 37oC, na kojima Listeria monocytogenes može uobičajeno da raste. Za navedena ispitivanja korišč ene su tri hranljive podloge različitog sastava, kao što su tripton soja bujon sa kvaščevim ekstraktom (TSB-YE), moždano srčana infuzija (BHI) i tripton soja bujon sa kvaščevim ekstraktom razređen u odnosu 1:20 fiziološkim rastvorom (1/20 TSB-YE). Za ispitivanja formiranja biofilma in vitro pripreman je inokulum od kultura izolovanih sojeva L. monocytogenes starih 24 časa. Gustina pripremljenog inokuluma iznosila je 2-10x107 CFU/ml (OD600=0,093 ± 0,009 u TSB-YE). Mikrotitracione ploče nakon inokulisanja inkubisane su tokom 48 časova na prethodno navedenim temperaturama. Kolonizacija sojeva L. monocytogenes na polistiren i formiranje biofilma, praćeno je pomoću kristal violet boje koja je dodavana u mikrotitracione ploče, kao i pomoću svetlosnog mikroskopa. Sojevi Listeria monocytogenes koji su ispitivani pokazali su različitu sposobnost formiranja biofilma u zavisnosti od temperature inkubacije i podloge koja je korišćena za rast. Zbog navedenih činjenica u radu su prikazani rezultati dobijeni ispitivanjem četiri soja Listeria monocytogenes koji su izolovani iz uzoraka mozga obolelih ovaca i označeni brojevima 785/05, 593/05, 748/05, 1915/04 i jedan soj izolovan iz pobač enog fetusa krave označen brojem 021/04, kao i jednog referentnog soja L. monocytogenes 1071 (4b) koji je korišćen kao kontrola. Sojevi L. monocytogenes koji su bili zasejavani u podloge bogatije hranljivim materijama, kao što je (TSBYE) i kultivisani na višim temperaturama (na temperaturi od 37 oC) bolje su formirali biofilm. Nijedan od ispitivanih sojeva nije mogao biti procenjen kao jak biofilm "producer", bez obzira na temperaturu inkubacije ili korišćenu podlogu. Najviše OD vrednosti dobijene su na temperaturi inkubacije od 37oC u TSB-YE (OD595=0,346 ±0,055), kada su tri ispitana soja kategorisana kao umereni, a dva kao slabi produktori biofilma. Sa smanjenjem temperature inkubacije u istoj podlozi, smanjivale su se i OD vrednosti, pa su na temperaturi od 25oC (OD595=0,289 ± 0,083) četiri soja procenjena kao slabi biofilm, a na temperaturi od 4oC (OD595=0,124 ± 0,011) nijedan soj nije procenjen kao produktor biofilma. Kvantitativne vrednosti dobijene primenom mikrotitracionog testa sa kristal violet bojom, koji se pokazao kao jednostavna i brza (skrining) metoda za procenu sposobnosti sojeva L. monocytogenes da formiraju biofilm pod različitim uslovima, potvrđene su i primenom svetlosne mikroskopije.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Investigation of biofilm formation in vitro ability of Listeria monocytogenes strains isolated from animals, Ispitivanje sposobnosti formiranja biofilma u uslovima in vitro sojeva Listeria monocytogenes izolovanih od životinja",
volume = "57",
number = "5-6",
pages = "429-440",
doi = "10.2298/AVB0706429M"
}

Milanov, D., Ašanin, R., Mišić, D., Vidić, B.,& Ratajac, R.. (2007). Investigation of biofilm formation in vitro ability of Listeria monocytogenes strains isolated from animals. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(5-6), 429-440.
https://doi.org/10.2298/AVB0706429M

Milanov D, Ašanin R, Mišić D, Vidić B, Ratajac R. Investigation of biofilm formation in vitro ability of Listeria monocytogenes strains isolated from animals. in Acta Veterinaria-Beograd. 2007;57(5-6):429-440.
doi:10.2298/AVB0706429M .

Milanov, Dubravka, Ašanin, Ružica, Mišić, Dušan, Vidić, Branka, Ratajac, Radomir, "Investigation of biofilm formation in vitro ability of Listeria monocytogenes strains isolated from animals" in Acta Veterinaria-Beograd, 57, no. 5-6 (2007):429-440,
https://doi.org/10.2298/AVB0706429M . .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Ašanin, Ružica
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/424
AB  - The investigation of the presence of plasmids was performed on E. coli and Salmonella strains, originating from dogs, cats, cattle, pigs and poultry. The investigated bacterial strains were isolated from ear and skin swabs vaginal swabs, faeces and urine, obtained from healthy and diseased animals of various age and breed categories. Up to now 45 E. coli and 35 Salmonella strains have been isolated, but the presence of plasmids was investigated in 42 strains of bacteria resistant to at least 3 or more antibiotics (23 E. coli and 4 Salmonella strains). The plasmids were isolated from 2 E. coli strains originating from dogs, 4 E. coli strains originating from cattle, 2 E. coli strains originating from pigs, 1 E. coli strain originating from a cat and 2 Salmonella strains originating from poultry. The obtained approximate size values of isolated plasmids from investigated strains expressed in base pairs were from 2,4 kb to 17,5 kb. After the transformation process was completed and the E. coli DH 5α transformants streaking was done on LB agar with added antibiotics, the visible growth of transformed bacteria meant that the competent cells have received DNA (plasmid) with the resistance gene for the antibiotic. Lack of growth in transformants meant that the resistance gene was to be found on the chromosome.
AB  - Ispitivanje prisustva plazmida vršeno je na sojevima E. coli i Salmonella vrsta poreklom od pasa, mačaka, goveda, svinja i živine. Ispitivani sojevi bakterija izolovani su iz briseva ušiju, kože, vaginalnih briseva, fecesa i urina i to kako od zdravih tako i od bolesnih jedinki različitih starosnih kategorija i rasa. Ukupno je izolovano 45 sojeva E. coli i 35 sojeva Salmonella vrsta a prisustvo plazmida ispitano je kod 42 soja bakterija rezistentnih na najmanje 3 i više antibiotika. Plazmidi su izolovani iz 11 ispitanih sojeva i to iz 2 soja E. coli poreklom od pasa, 4 soja E. coli poreklom od goveda, 2 soja E. coli poreklom od svinja, 1 soja E. coli poreklom od mačke i kod 2 soja Salmonella vrsta poreklom od živine. Nakon izvršene transformacije i zasejavanja transformanata E. coli DH 5α na LB agar sa dodatkom antibiotika, vidljivi porast transformisanih bakterija značio je da su kompetentne ćelije primile DNK (plazmid) sa genom rezistencije na taj antibiotik. Izostanak porasta transformanata značio je da se gen rezistencije nalazio na hromozomu.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Investigation of the resistance gene presence in plasmids isolated from e. Coli and salmonella strains originating from domestic animals by the method of plasmid DNA transformation into competent cell
T1  - Ispitivanje prisustva gena rezistencije na plazmidima izolovanim iz sojeva e. Coli i salmonella vrsta poreklom od domaćih životinja metodom transformacije plazmidske DNK u kompetentne ćelije
VL  - 56
IS  - 4
SP  - 315
EP  - 322
DO  - 10.2298/AVB0604315M
ER  - 

@article{
author = "Mišić, Dušan and Ašanin, Ružica",
year = "2006",
abstract = "The investigation of the presence of plasmids was performed on E. coli and Salmonella strains, originating from dogs, cats, cattle, pigs and poultry. The investigated bacterial strains were isolated from ear and skin swabs vaginal swabs, faeces and urine, obtained from healthy and diseased animals of various age and breed categories. Up to now 45 E. coli and 35 Salmonella strains have been isolated, but the presence of plasmids was investigated in 42 strains of bacteria resistant to at least 3 or more antibiotics (23 E. coli and 4 Salmonella strains). The plasmids were isolated from 2 E. coli strains originating from dogs, 4 E. coli strains originating from cattle, 2 E. coli strains originating from pigs, 1 E. coli strain originating from a cat and 2 Salmonella strains originating from poultry. The obtained approximate size values of isolated plasmids from investigated strains expressed in base pairs were from 2,4 kb to 17,5 kb. After the transformation process was completed and the E. coli DH 5α transformants streaking was done on LB agar with added antibiotics, the visible growth of transformed bacteria meant that the competent cells have received DNA (plasmid) with the resistance gene for the antibiotic. Lack of growth in transformants meant that the resistance gene was to be found on the chromosome., Ispitivanje prisustva plazmida vršeno je na sojevima E. coli i Salmonella vrsta poreklom od pasa, mačaka, goveda, svinja i živine. Ispitivani sojevi bakterija izolovani su iz briseva ušiju, kože, vaginalnih briseva, fecesa i urina i to kako od zdravih tako i od bolesnih jedinki različitih starosnih kategorija i rasa. Ukupno je izolovano 45 sojeva E. coli i 35 sojeva Salmonella vrsta a prisustvo plazmida ispitano je kod 42 soja bakterija rezistentnih na najmanje 3 i više antibiotika. Plazmidi su izolovani iz 11 ispitanih sojeva i to iz 2 soja E. coli poreklom od pasa, 4 soja E. coli poreklom od goveda, 2 soja E. coli poreklom od svinja, 1 soja E. coli poreklom od mačke i kod 2 soja Salmonella vrsta poreklom od živine. Nakon izvršene transformacije i zasejavanja transformanata E. coli DH 5α na LB agar sa dodatkom antibiotika, vidljivi porast transformisanih bakterija značio je da su kompetentne ćelije primile DNK (plazmid) sa genom rezistencije na taj antibiotik. Izostanak porasta transformanata značio je da se gen rezistencije nalazio na hromozomu.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Investigation of the resistance gene presence in plasmids isolated from e. Coli and salmonella strains originating from domestic animals by the method of plasmid DNA transformation into competent cell, Ispitivanje prisustva gena rezistencije na plazmidima izolovanim iz sojeva e. Coli i salmonella vrsta poreklom od domaćih životinja metodom transformacije plazmidske DNK u kompetentne ćelije",
volume = "56",
number = "4",
pages = "315-322",
doi = "10.2298/AVB0604315M"
}

Mišić, D.,& Ašanin, R.. (2006). Investigation of the resistance gene presence in plasmids isolated from e. Coli and salmonella strains originating from domestic animals by the method of plasmid DNA transformation into competent cell. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(4), 315-322.
https://doi.org/10.2298/AVB0604315M

Mišić D, Ašanin R. Investigation of the resistance gene presence in plasmids isolated from e. Coli and salmonella strains originating from domestic animals by the method of plasmid DNA transformation into competent cell. in Acta Veterinaria-Beograd. 2006;56(4):315-322.
doi:10.2298/AVB0604315M .

Mišić, Dušan, Ašanin, Ružica, "Investigation of the resistance gene presence in plasmids isolated from e. Coli and salmonella strains originating from domestic animals by the method of plasmid DNA transformation into competent cell" in Acta Veterinaria-Beograd, 56, no. 4 (2006):315-322,
https://doi.org/10.2298/AVB0604315M . .

TY  - JOUR
AU  - Stošić, Zorica
AU  - Mitrić, Milan
AU  - Kiskároly, Ferenc
AU  - Mišić, Dušan
AU  - Ašanin, Ružica
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/381
AB  - A total of 1666 samples were examined, of which 512 samples of parenchymatous organs of dead or deliberately sacrtificed animals, 60 samples of non-hatched fertilized eggs, 202 samples of feces, 652 samples of cloacal smears, 221 samples of smears from walls of maintenance objects, incubator stations, and transport vehicles, 19 samples of beddings and shavings. The samples originated from poultry farms and which were taken to a laboratory immediately on sampling and sown the same day. A total of 104 strains of Salmonella were isolated: 94 strains from samples of parenchymatous organs of dead chicks, 1 strain from non-hatched eggs, 3 strains from feces samples, 1 strain from samples of cloacal smears, 4 strains from samples of surface smears of maintenance objects and transport vehicles, and 1 strain from samples of beddings and shavings. Serological typization established the presence of the following serovarieties: Salmonella Enteritidis 79 strains, Salmonella Hartford 17 strains, Salmonella Typohimurium 5 strains, Salmonella Mbandaka 2 strains, and Salmonella Glostrup 1 strain. We examined the sensitivity of Salmonella strains to ampicillin, amoxicillin, gentamycin, streptomycin, neomycin, enrofloxacine, norfloxacine, flumequin, erythromycin, lincospectin, colistin, fluorphenicol, and a combination of sulphamethoxasole and trimethoprim. In S. Enteritidis strains, no resistence was established to colistin, fluorphenicol and sulphamethoxasole+trimethoprim, in fact, the sensitivity to these antibiotics and chemotherapeutics was 100%. Prevalence resitence of 0.96%, in only one strain, was established for enrofloxacine. A high prevalence resistence of 33.6% was established for neomycin, while prevalence resistence of 3.86% was established for the related aminoglycozide antibiotic gentamycin. The highest prevalence resistance in S.Hartford strains was established for erythromycin, 15.38%, and streptomycin, 7.6%. Resistence of S. Tyohimurium was established for flumequin and erythromycin in 1.9% strains. No resistance to antibiotics was established in the strains S. Mbandaka and S. Glostrup.
AB  - Ispitano je ukupno 1666 uzoraka. Od toga 512 uzoraka parenhimatoznih organa uginulih ili prinudno žrtvovanih jedinki, 60 uzoraka oplođenih jaja iz kojih se nisu izlegli pilići (ugušaka), 202 uzorka fecesa, 652 uzorka kloakalnih briseva, 221 uzorak briseva sa zidova objekata za odgoj, inkubatorskih stanica i transportnih vozila i 19 uzoraka prostirke i šuške. Uzorci su poticali sa farmi živine i odmah nakon uzorkovanja dopremani su u laboratoriju i istog dana zasejavani. Ukupno je izolovano 104 soja salmonela: 94 soja iz uzoraka parenhi-matoznih organa uginulih ptica, jedan soj iz uzoraka ugušaka jaja, tri soja iz uzoraka fecesa, jedan soj iz uzoraka kloakalnih briseva, četiri soja iz uzoraka briseva sa površina objekata za odgoj i transportnih vozila i jedan soj iz uzoraka prostirke i šuške. Serološkom tipizacijom ustanovljeno je prisustvo sledećih serovarijeteta: Salmonella Enteritidis 79 sojeva, Salmonella Hartford 17 sojeva, Salmonella Typhimurium pet sojeva, Salmonella Mbandaka dva soja i Salmonella Glostrup jedan soj. Ispitana je osetljivost sojeva Salmonella na ampicilin, amoksicilin, gentamicin, streptomicin, neomicin, enrofloksacin, norfloksacin, flumekvin, eritromicin, linkospektin, kolistin, fluorfenikol i kombinaciju sulfametoksazola i trimetoprima. Kod sojeva S. Enteritidis, nije otkrivena rezistencija na kolistin, fluorfenikol i sulfametoksazol+trimetoprim, odnosno, osetljivost na ove antibakterijske lekove bila je sto posto. Prevalencija rezistencije od 0,96%, odnosno samo kod jednog soja utvrđena je na enrofloksacin. Visoka prevalencija rezistencije od 33,6% ustanovljena je na neomicin, dok je prevalencija rezistencije od 3,86 posto ustanovljena na srodni aminoglikozidni antibiotik gentamicin. Najviša prevalencija rezistencije kod sojeva S. Hartford ustanovljena je na eritromicin od 15,38 posto i streptomicin od 7,6 posto. Rezistencija S. Typhimurium otkrivena je na flumekvin i eritromicin kod 1,9 posto sojeva. Kod sojeva S. Mbandaka i S. Glostrup nije ustanovljena rezistencija na antibiotike.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Investigations of sensitivity to antibiotics of salmonella strain species originating from poultry from different epizootiological areas
T1  - Ispitivanje osetljivosti sojeva salmonella vrsta poreklom od živine sa različitih epizootioloških područja na antibiotike
VL  - 60
IS  - 1-2
SP  - 89
EP  - 98
DO  - 10.2298/VETGL0602089S
ER  - 

@article{
author = "Stošić, Zorica and Mitrić, Milan and Kiskároly, Ferenc and Mišić, Dušan and Ašanin, Ružica",
year = "2006",
abstract = "A total of 1666 samples were examined, of which 512 samples of parenchymatous organs of dead or deliberately sacrtificed animals, 60 samples of non-hatched fertilized eggs, 202 samples of feces, 652 samples of cloacal smears, 221 samples of smears from walls of maintenance objects, incubator stations, and transport vehicles, 19 samples of beddings and shavings. The samples originated from poultry farms and which were taken to a laboratory immediately on sampling and sown the same day. A total of 104 strains of Salmonella were isolated: 94 strains from samples of parenchymatous organs of dead chicks, 1 strain from non-hatched eggs, 3 strains from feces samples, 1 strain from samples of cloacal smears, 4 strains from samples of surface smears of maintenance objects and transport vehicles, and 1 strain from samples of beddings and shavings. Serological typization established the presence of the following serovarieties: Salmonella Enteritidis 79 strains, Salmonella Hartford 17 strains, Salmonella Typohimurium 5 strains, Salmonella Mbandaka 2 strains, and Salmonella Glostrup 1 strain. We examined the sensitivity of Salmonella strains to ampicillin, amoxicillin, gentamycin, streptomycin, neomycin, enrofloxacine, norfloxacine, flumequin, erythromycin, lincospectin, colistin, fluorphenicol, and a combination of sulphamethoxasole and trimethoprim. In S. Enteritidis strains, no resistence was established to colistin, fluorphenicol and sulphamethoxasole+trimethoprim, in fact, the sensitivity to these antibiotics and chemotherapeutics was 100%. Prevalence resitence of 0.96%, in only one strain, was established for enrofloxacine. A high prevalence resistence of 33.6% was established for neomycin, while prevalence resistence of 3.86% was established for the related aminoglycozide antibiotic gentamycin. The highest prevalence resistance in S.Hartford strains was established for erythromycin, 15.38%, and streptomycin, 7.6%. Resistence of S. Tyohimurium was established for flumequin and erythromycin in 1.9% strains. No resistance to antibiotics was established in the strains S. Mbandaka and S. Glostrup., Ispitano je ukupno 1666 uzoraka. Od toga 512 uzoraka parenhimatoznih organa uginulih ili prinudno žrtvovanih jedinki, 60 uzoraka oplođenih jaja iz kojih se nisu izlegli pilići (ugušaka), 202 uzorka fecesa, 652 uzorka kloakalnih briseva, 221 uzorak briseva sa zidova objekata za odgoj, inkubatorskih stanica i transportnih vozila i 19 uzoraka prostirke i šuške. Uzorci su poticali sa farmi živine i odmah nakon uzorkovanja dopremani su u laboratoriju i istog dana zasejavani. Ukupno je izolovano 104 soja salmonela: 94 soja iz uzoraka parenhi-matoznih organa uginulih ptica, jedan soj iz uzoraka ugušaka jaja, tri soja iz uzoraka fecesa, jedan soj iz uzoraka kloakalnih briseva, četiri soja iz uzoraka briseva sa površina objekata za odgoj i transportnih vozila i jedan soj iz uzoraka prostirke i šuške. Serološkom tipizacijom ustanovljeno je prisustvo sledećih serovarijeteta: Salmonella Enteritidis 79 sojeva, Salmonella Hartford 17 sojeva, Salmonella Typhimurium pet sojeva, Salmonella Mbandaka dva soja i Salmonella Glostrup jedan soj. Ispitana je osetljivost sojeva Salmonella na ampicilin, amoksicilin, gentamicin, streptomicin, neomicin, enrofloksacin, norfloksacin, flumekvin, eritromicin, linkospektin, kolistin, fluorfenikol i kombinaciju sulfametoksazola i trimetoprima. Kod sojeva S. Enteritidis, nije otkrivena rezistencija na kolistin, fluorfenikol i sulfametoksazol+trimetoprim, odnosno, osetljivost na ove antibakterijske lekove bila je sto posto. Prevalencija rezistencije od 0,96%, odnosno samo kod jednog soja utvrđena je na enrofloksacin. Visoka prevalencija rezistencije od 33,6% ustanovljena je na neomicin, dok je prevalencija rezistencije od 3,86 posto ustanovljena na srodni aminoglikozidni antibiotik gentamicin. Najviša prevalencija rezistencije kod sojeva S. Hartford ustanovljena je na eritromicin od 15,38 posto i streptomicin od 7,6 posto. Rezistencija S. Typhimurium otkrivena je na flumekvin i eritromicin kod 1,9 posto sojeva. Kod sojeva S. Mbandaka i S. Glostrup nije ustanovljena rezistencija na antibiotike.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Investigations of sensitivity to antibiotics of salmonella strain species originating from poultry from different epizootiological areas, Ispitivanje osetljivosti sojeva salmonella vrsta poreklom od živine sa različitih epizootioloških područja na antibiotike",
volume = "60",
number = "1-2",
pages = "89-98",
doi = "10.2298/VETGL0602089S"
}

Stošić, Z., Mitrić, M., Kiskároly, F., Mišić, D.,& Ašanin, R.. (2006). Investigations of sensitivity to antibiotics of salmonella strain species originating from poultry from different epizootiological areas. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(1-2), 89-98.
https://doi.org/10.2298/VETGL0602089S

Stošić Z, Mitrić M, Kiskároly F, Mišić D, Ašanin R. Investigations of sensitivity to antibiotics of salmonella strain species originating from poultry from different epizootiological areas. in Veterinarski Glasnik. 2006;60(1-2):89-98.
doi:10.2298/VETGL0602089S .

Stošić, Zorica, Mitrić, Milan, Kiskároly, Ferenc, Mišić, Dušan, Ašanin, Ružica, "Investigations of sensitivity to antibiotics of salmonella strain species originating from poultry from different epizootiological areas" in Veterinarski Glasnik, 60, no. 1-2 (2006):89-98,
https://doi.org/10.2298/VETGL0602089S . .

TY  - JOUR
AU  - Mišić, Dušan
AU  - Stošić, Zorica
AU  - Kiskároly, Ferenc
AU  - Adamov, Vladica
AU  - Ašanin, Ružica
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/386
AB  - The presence of multiresistance to the effects of antibiotics and chemotherapeutics and extended spectrum beta-lactamase were examined in 45 strains of E. coli and 35 strains of Salmonella. The strains of E. coli originated from several species of domestic animals: dogs, cats, poultry, and cattle, and 30 strains of Salmonella originated from poultry, 4 strains from cattle, and 1 strain from swine. The presence of the following serovarieties was established using serological examinations: Salmonella Enteritidis 17 strains, Salmonella Gallinarum 1 strain, Salmonella Hartford 5 strains, Salmonella Anatum 1 strain, Salmonella Typhimurium 4 strains, Salmonella Agona 1 strain, Salmonella Infantis 1 strain, Salmonella Thompson var. Berlin 1 strain, Salmonella Tennessee 1 strain, Salmonella Senftenberg 1 strain, Salmonella Glostrup 1 strain, and Salmonella Hadar 1 strain. In the examinations of the listed strains we used antibiogram discs of ampicillin, amoxicillin with clavulanic acid, cephalexin, cephtriaxon, cephotaxim, cephtazidime, aztreonam, gentamycin, chloramphenicol, tetracycline, cyprofloxacine, and a combination of sulphamethoxasole and trimethoprim. The lowest prevalence of multiresistance in E. Coli strains to 3 or more antibiotics was established in dogs 20%, and the highest in 60% strains originating from swine. In 62.88% strains of Salmonella we established sensitivity to all applied antibiotics. Resistance was also established in a small number of the examined strains to ampicillin (11 strains), to tetracycline (5 strains), to amoxicillin with clavulanic acid (5 strains), to sulphamethoxasole with trimethoprim (5 strains), to gentamycin (3 strains), and to cloramphenicol (1 strain). Of all the examined strains of Salmonella, 6 strains originating from poultry exhibited multiresistence. The presence of extended spectrum beta-lactamase effects examined using the ESBL test, was not established in strains of E. coli and Salmonella strains.
AB  - Ispitivano je prisustvo multirezistencije na antibiotike i hemioterapeutike i beta laktamaze proširenog spektra delovanja kod 45 sojeva E. coli i 35 sojeva Salmonella. Sojevi E. coli su poticali od vise vrsta domaćih životinja: pasa, mačaka, živine i goveda, a 30 sojeva salmonela poticalo je od živine, četiri soja od goveda i jedan soj od svinja. Serološkom determinacijom ustanovljeno je prisustvo navedenih serovarijeteta: Salmonella Enteritidis 17 sojeva, Salmonella Gallinarum 1 soj, Salmonella Hartford 5 sojeva, Salmonella Anatum 1 soj, Salmonella Typhimurium 4 soja, Salmonella Agona 1 soj, Salmonella Infantis 1 soj, Salmonella Thompson var. Berlin 1 soj, Salmonella Tennessee 1 soj, Salmonella Senftenberg 1 soj, Salmonella Glostrup 1 soj i Salmonella Hadar 1 soj. 2a ispitivanje navedenih sojeva su korišćeni antibiogram diskovi ampicilina, amoksicilina sa klavulanskom kiselinom, cefaleksina, ceftriaksona, cefotaksima, ceftazidima, aztreonama, gentamicina, hloramfenikola, tetraciklina, ciprofloksacina i kombinacija sulfametoksazola i trimetoprima. Najniža prevaiencija multirezistencije kod sojeva E. coli na tri ili vise antibiotika ustanovljena je kod pasa 20 posto, a najviša kod 60 posto sojeva koji potiču od svinja. Kod 62,88 posto sojeva salmonela ustanovljena je osetljivost na sve primenjene antibiotike. Rezistencija je utvrđena kod malog broja ispitivanih sojeva i to na ampicilin (11 sojeva), na tetraciklin (5 sojeva), na amoksicilin sa klavulanskom kiselinom (5 sojeva) na sulfametoksazol sa trimetoprimorn (5 sojeva), na gentamicin (3 soja) i na hloramfentkol (1 soj). Od svih ispitanih sojeva salmonela, 6 sojeva koji potiču od živine ispoljilo je multirezistenciju. Prisustvo beta-taktamaza proširenog spektra delovanja ispitanog primenom ESBL testa nije ustanovljeno kod sojeva E. coli i Salmonella.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Investigations of multiresistance to antibiotics and chemotherapeutics and extended spectrum beta: Lactamase effect (ESBL test) in strains E.coli and salmonella originating from domestic animals
T1  - Ispitivanje multirezistencije E.coli i Salmonella koje potiču od domaćih životinja na antibiotike i hemioterapeutike
VL  - 60
IS  - 1-2
SP  - 21
EP  - 31
DO  - 10.2298/VETGL0602021M
ER  - 

@article{
author = "Mišić, Dušan and Stošić, Zorica and Kiskároly, Ferenc and Adamov, Vladica and Ašanin, Ružica",
year = "2006",
abstract = "The presence of multiresistance to the effects of antibiotics and chemotherapeutics and extended spectrum beta-lactamase were examined in 45 strains of E. coli and 35 strains of Salmonella. The strains of E. coli originated from several species of domestic animals: dogs, cats, poultry, and cattle, and 30 strains of Salmonella originated from poultry, 4 strains from cattle, and 1 strain from swine. The presence of the following serovarieties was established using serological examinations: Salmonella Enteritidis 17 strains, Salmonella Gallinarum 1 strain, Salmonella Hartford 5 strains, Salmonella Anatum 1 strain, Salmonella Typhimurium 4 strains, Salmonella Agona 1 strain, Salmonella Infantis 1 strain, Salmonella Thompson var. Berlin 1 strain, Salmonella Tennessee 1 strain, Salmonella Senftenberg 1 strain, Salmonella Glostrup 1 strain, and Salmonella Hadar 1 strain. In the examinations of the listed strains we used antibiogram discs of ampicillin, amoxicillin with clavulanic acid, cephalexin, cephtriaxon, cephotaxim, cephtazidime, aztreonam, gentamycin, chloramphenicol, tetracycline, cyprofloxacine, and a combination of sulphamethoxasole and trimethoprim. The lowest prevalence of multiresistance in E. Coli strains to 3 or more antibiotics was established in dogs 20%, and the highest in 60% strains originating from swine. In 62.88% strains of Salmonella we established sensitivity to all applied antibiotics. Resistance was also established in a small number of the examined strains to ampicillin (11 strains), to tetracycline (5 strains), to amoxicillin with clavulanic acid (5 strains), to sulphamethoxasole with trimethoprim (5 strains), to gentamycin (3 strains), and to cloramphenicol (1 strain). Of all the examined strains of Salmonella, 6 strains originating from poultry exhibited multiresistence. The presence of extended spectrum beta-lactamase effects examined using the ESBL test, was not established in strains of E. coli and Salmonella strains., Ispitivano je prisustvo multirezistencije na antibiotike i hemioterapeutike i beta laktamaze proširenog spektra delovanja kod 45 sojeva E. coli i 35 sojeva Salmonella. Sojevi E. coli su poticali od vise vrsta domaćih životinja: pasa, mačaka, živine i goveda, a 30 sojeva salmonela poticalo je od živine, četiri soja od goveda i jedan soj od svinja. Serološkom determinacijom ustanovljeno je prisustvo navedenih serovarijeteta: Salmonella Enteritidis 17 sojeva, Salmonella Gallinarum 1 soj, Salmonella Hartford 5 sojeva, Salmonella Anatum 1 soj, Salmonella Typhimurium 4 soja, Salmonella Agona 1 soj, Salmonella Infantis 1 soj, Salmonella Thompson var. Berlin 1 soj, Salmonella Tennessee 1 soj, Salmonella Senftenberg 1 soj, Salmonella Glostrup 1 soj i Salmonella Hadar 1 soj. 2a ispitivanje navedenih sojeva su korišćeni antibiogram diskovi ampicilina, amoksicilina sa klavulanskom kiselinom, cefaleksina, ceftriaksona, cefotaksima, ceftazidima, aztreonama, gentamicina, hloramfenikola, tetraciklina, ciprofloksacina i kombinacija sulfametoksazola i trimetoprima. Najniža prevaiencija multirezistencije kod sojeva E. coli na tri ili vise antibiotika ustanovljena je kod pasa 20 posto, a najviša kod 60 posto sojeva koji potiču od svinja. Kod 62,88 posto sojeva salmonela ustanovljena je osetljivost na sve primenjene antibiotike. Rezistencija je utvrđena kod malog broja ispitivanih sojeva i to na ampicilin (11 sojeva), na tetraciklin (5 sojeva), na amoksicilin sa klavulanskom kiselinom (5 sojeva) na sulfametoksazol sa trimetoprimorn (5 sojeva), na gentamicin (3 soja) i na hloramfentkol (1 soj). Od svih ispitanih sojeva salmonela, 6 sojeva koji potiču od živine ispoljilo je multirezistenciju. Prisustvo beta-taktamaza proširenog spektra delovanja ispitanog primenom ESBL testa nije ustanovljeno kod sojeva E. coli i Salmonella.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Investigations of multiresistance to antibiotics and chemotherapeutics and extended spectrum beta: Lactamase effect (ESBL test) in strains E.coli and salmonella originating from domestic animals, Ispitivanje multirezistencije E.coli i Salmonella koje potiču od domaćih životinja na antibiotike i hemioterapeutike",
volume = "60",
number = "1-2",
pages = "21-31",
doi = "10.2298/VETGL0602021M"
}

Mišić, D., Stošić, Z., Kiskároly, F., Adamov, V.,& Ašanin, R.. (2006). Investigations of multiresistance to antibiotics and chemotherapeutics and extended spectrum beta: Lactamase effect (ESBL test) in strains E.coli and salmonella originating from domestic animals. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(1-2), 21-31.
https://doi.org/10.2298/VETGL0602021M

Mišić D, Stošić Z, Kiskároly F, Adamov V, Ašanin R. Investigations of multiresistance to antibiotics and chemotherapeutics and extended spectrum beta: Lactamase effect (ESBL test) in strains E.coli and salmonella originating from domestic animals. in Veterinarski Glasnik. 2006;60(1-2):21-31.
doi:10.2298/VETGL0602021M .

Mišić, Dušan, Stošić, Zorica, Kiskároly, Ferenc, Adamov, Vladica, Ašanin, Ružica, "Investigations of multiresistance to antibiotics and chemotherapeutics and extended spectrum beta: Lactamase effect (ESBL test) in strains E.coli and salmonella originating from domestic animals" in Veterinarski Glasnik, 60, no. 1-2 (2006):21-31,
https://doi.org/10.2298/VETGL0602021M . .