TY  - CONF
AU  - Savić Radovanović, Radoslava
AU  - Rajić-Savić, Nataša
AU  - Gajić, Ina
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2320
AB  - Pseudomonas spp.as ubiquitous microorganism is often found in environmental raw materials as a
contaminant. P. aeruginosa and P. fluorescens but also P. putida, P. fragi and P. cochorii may be
isolated from milk and meet. From the view point of food hygiene synthesis of thermostable
lipolytic and proteolytic enzymes in the cold chain of food production, can cause the spoilage of
final product. As a causative agent of nosocomial infections, Pseudomonas spp. are often resistant
to a large number of antimicrobial substances. Due to their ubiquity and ability to acquire resistance
represents a potential risk to human health. The aims of the study were to assess antimicrobial
susceptibility of Pseudomonas spp. isolated from food and to evaluate their proteolytic and lipolytic
activity. A total of 40 isolates (20 from raw milk; 20 from carcasses of slaughtered pigs) were
examined. All of the isolates were oxidase and, catalase positive, produced a pigment on Tryptone
Soy Agar and had a characteristic odour. They also showed haemolysis on Blood agar, lipolysis on
Tributyrin and proteolysis on Casein agar. Antimicrobial susceptibility testing was performed by
disk diffusion test on piperacillin/ tazobactam, ticarcillin, imipenem, meropenem, aztreonam,
amikacin, gentamicin, levofloxacin and ceftazidime. The sensitivity of milk isolates was 100%;
65%; 100%; 100%; 25%; 75%; 30%; 65% and 100%, whereas the susceptibility of carcass-derived
isolates was 95%; 55%; 95%; 95%, 0%; 95%, 10%, 25% and 100%. Susceptibility of milk and
carcass-derived isolates to piperacillin/tazobactam, ticarcillin, imipenem, meropenem, aztreonam,
amikacin, gentamicin, levofloxacin and ceftazidime was 100%, 65%, 100%, 100%, 25%, 75%,
30%, 65%, 100% and 95%, 55%, 95%, 95%, 0%, 95%, 10%, 25%, 100%, respectively. There was
no resistance to ceftazidime indicating no ESBL strains. MDR strains resistant to three or more
antibiotics were 2 (10%) from milk and 4 (20%) from meat, namely ticarcillin, gentamicin,
levofloxacin in milk isolates and ticarcillin, gentamicin, levofloxacin in three and piperacillin /
tazobactam, ticarcillin, gentamicin, levofloxacin of one carcase isolate. In the present study, four
MDR isolates were detected, 2 (10%) being isolated from milk and 4 (20%) from meat. Their
resistance patterns were as follows: resistance to ticarcillin, gentamicin, levofloxacin (milk: n=2);
resistance to ticarcillin, gentamicin, levofloxacin (carcase: n=3), resistance to
piperacillin/tazobactam, ticarcillin, gentamicin, levofloxacin (carcase: n=1). In conclusion,
pseudomonads might contaminate raw food of animal origin consequently leading to spoilage and
considered as a reservoir of Pseudomonas spp. resistome.
PB  - University of Belgrade
C3  - 2nd International UNIfood Conference
T1  - Characteristics of Pseudomonas spp. isolated from food of animal origin
SP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2320
ER  - 

@conference{
author = "Savić Radovanović, Radoslava and Rajić-Savić, Nataša and Gajić, Ina",
year = "2021",
abstract = "Pseudomonas spp.as ubiquitous microorganism is often found in environmental raw materials as a
contaminant. P. aeruginosa and P. fluorescens but also P. putida, P. fragi and P. cochorii may be
isolated from milk and meet. From the view point of food hygiene synthesis of thermostable
lipolytic and proteolytic enzymes in the cold chain of food production, can cause the spoilage of
final product. As a causative agent of nosocomial infections, Pseudomonas spp. are often resistant
to a large number of antimicrobial substances. Due to their ubiquity and ability to acquire resistance
represents a potential risk to human health. The aims of the study were to assess antimicrobial
susceptibility of Pseudomonas spp. isolated from food and to evaluate their proteolytic and lipolytic
activity. A total of 40 isolates (20 from raw milk; 20 from carcasses of slaughtered pigs) were
examined. All of the isolates were oxidase and, catalase positive, produced a pigment on Tryptone
Soy Agar and had a characteristic odour. They also showed haemolysis on Blood agar, lipolysis on
Tributyrin and proteolysis on Casein agar. Antimicrobial susceptibility testing was performed by
disk diffusion test on piperacillin/ tazobactam, ticarcillin, imipenem, meropenem, aztreonam,
amikacin, gentamicin, levofloxacin and ceftazidime. The sensitivity of milk isolates was 100%;
65%; 100%; 100%; 25%; 75%; 30%; 65% and 100%, whereas the susceptibility of carcass-derived
isolates was 95%; 55%; 95%; 95%, 0%; 95%, 10%, 25% and 100%. Susceptibility of milk and
carcass-derived isolates to piperacillin/tazobactam, ticarcillin, imipenem, meropenem, aztreonam,
amikacin, gentamicin, levofloxacin and ceftazidime was 100%, 65%, 100%, 100%, 25%, 75%,
30%, 65%, 100% and 95%, 55%, 95%, 95%, 0%, 95%, 10%, 25%, 100%, respectively. There was
no resistance to ceftazidime indicating no ESBL strains. MDR strains resistant to three or more
antibiotics were 2 (10%) from milk and 4 (20%) from meat, namely ticarcillin, gentamicin,
levofloxacin in milk isolates and ticarcillin, gentamicin, levofloxacin in three and piperacillin /
tazobactam, ticarcillin, gentamicin, levofloxacin of one carcase isolate. In the present study, four
MDR isolates were detected, 2 (10%) being isolated from milk and 4 (20%) from meat. Their
resistance patterns were as follows: resistance to ticarcillin, gentamicin, levofloxacin (milk: n=2);
resistance to ticarcillin, gentamicin, levofloxacin (carcase: n=3), resistance to
piperacillin/tazobactam, ticarcillin, gentamicin, levofloxacin (carcase: n=1). In conclusion,
pseudomonads might contaminate raw food of animal origin consequently leading to spoilage and
considered as a reservoir of Pseudomonas spp. resistome.",
publisher = "University of Belgrade",
journal = "2nd International UNIfood Conference",
title = "Characteristics of Pseudomonas spp. isolated from food of animal origin",
pages = "124",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2320"
}

Savić Radovanović, R., Rajić-Savić, N.,& Gajić, I.. (2021). Characteristics of Pseudomonas spp. isolated from food of animal origin. in 2nd International UNIfood Conference
University of Belgrade., 124.
https://hdl.handle.net/21.15107/rcub_veterinar_2320

Savić Radovanović R, Rajić-Savić N, Gajić I. Characteristics of Pseudomonas spp. isolated from food of animal origin. in 2nd International UNIfood Conference. 2021;:124.
https://hdl.handle.net/21.15107/rcub_veterinar_2320 .

Savić Radovanović, Radoslava, Rajić-Savić, Nataša, Gajić, Ina, "Characteristics of Pseudomonas spp. isolated from food of animal origin" in 2nd International UNIfood Conference (2021):124,
https://hdl.handle.net/21.15107/rcub_veterinar_2320 .

TY  - JOUR
AU  - Savić Radovanović, Radoslava
AU  - Rajić Savić, Nataša
AU  - Ranin, Lazar
AU  - Smitran, Aleksandra
AU  - Vučković Opavski, Nataša
AU  - Milosavljević Tepavčević, Andreja
AU  - Ranin, Jovana
AU  - Gajić, Ina
PY  - 2020
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2697
AB  - Due to its ubiquity, ability to form biofilms, and acquire resistance mechanisms, Pseudomonas spp. become one of the major
challenge for healthcare settings and food industry. The aims of this study were to assess the biofilm production of Pseudomonas
spp. recovered from clinical and food specimens and to evaluate their antimicrobial resistance. A total of 108
isolates of Pseudomonas spp. were included in the study, 48 being clinical isolates recovered from patients admitted to four
tertiary care hospitals throughout Serbia and 60 were isolated from the bulk tank milk samples and meat carcasses. Biofilm
production was analyzed by microtiter plate assay. Antimicrobial susceptibility was evaluated by disk diffusion method
according to the CLSI guidelines, while class A and B β-lactamases encoding genes were screened by PCR. A total of 98
(90.7%) strains were biofilm producers (moderate producer: 68, 69.4%; strong producer: 8, 8.2%). Although a slightly higher
percentage of clinical isolates were biofilm producers (91.7%) compared to food isolates (90%), statistical significance was
not observed (P > 0.05). The proportion of carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates was significantly
higher among clinical (42%) isolates compared to food (1.7%) Pseudomonads (P < 0.05). The blaPER and blaNDM genes were
found in ESBL (seven isolates) and MBL (two isolates) production, respectively. In the present study, we confirmed that
biofilm formation was highly present in both clinical and food Pseudomonas spp. irrespective of the prior existence of resistance
genes. Additionally, clinical settings pose a major reservoir of MDR Pseudomonas spp. and especially CRPA isolates.
PB  - Springer
T2  - Current Microbiology
T1  - Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp.
VL  - 77
SP  - 4045
EP  - 4052
DO  - 10.1007/s00284-020-02236-4
ER  - 

@article{
author = "Savić Radovanović, Radoslava and Rajić Savić, Nataša and Ranin, Lazar and Smitran, Aleksandra and Vučković Opavski, Nataša and Milosavljević Tepavčević, Andreja and Ranin, Jovana and Gajić, Ina",
year = "2020",
abstract = "Due to its ubiquity, ability to form biofilms, and acquire resistance mechanisms, Pseudomonas spp. become one of the major
challenge for healthcare settings and food industry. The aims of this study were to assess the biofilm production of Pseudomonas
spp. recovered from clinical and food specimens and to evaluate their antimicrobial resistance. A total of 108
isolates of Pseudomonas spp. were included in the study, 48 being clinical isolates recovered from patients admitted to four
tertiary care hospitals throughout Serbia and 60 were isolated from the bulk tank milk samples and meat carcasses. Biofilm
production was analyzed by microtiter plate assay. Antimicrobial susceptibility was evaluated by disk diffusion method
according to the CLSI guidelines, while class A and B β-lactamases encoding genes were screened by PCR. A total of 98
(90.7%) strains were biofilm producers (moderate producer: 68, 69.4%; strong producer: 8, 8.2%). Although a slightly higher
percentage of clinical isolates were biofilm producers (91.7%) compared to food isolates (90%), statistical significance was
not observed (P > 0.05). The proportion of carbapenem-resistant Pseudomonas aeruginosa (CRPA) isolates was significantly
higher among clinical (42%) isolates compared to food (1.7%) Pseudomonads (P < 0.05). The blaPER and blaNDM genes were
found in ESBL (seven isolates) and MBL (two isolates) production, respectively. In the present study, we confirmed that
biofilm formation was highly present in both clinical and food Pseudomonas spp. irrespective of the prior existence of resistance
genes. Additionally, clinical settings pose a major reservoir of MDR Pseudomonas spp. and especially CRPA isolates.",
publisher = "Springer",
journal = "Current Microbiology",
title = "Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp.",
volume = "77",
pages = "4045-4052",
doi = "10.1007/s00284-020-02236-4"
}

Savić Radovanović, R., Rajić Savić, N., Ranin, L., Smitran, A., Vučković Opavski, N., Milosavljević Tepavčević, A., Ranin, J.,& Gajić, I.. (2020). Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp.. in Current Microbiology
Springer., 77, 4045-4052.
https://doi.org/10.1007/s00284-020-02236-4

Savić Radovanović R, Rajić Savić N, Ranin L, Smitran A, Vučković Opavski N, Milosavljević Tepavčević A, Ranin J, Gajić I. Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp.. in Current Microbiology. 2020;77:4045-4052.
doi:10.1007/s00284-020-02236-4 .

Savić Radovanović, Radoslava, Rajić Savić, Nataša, Ranin, Lazar, Smitran, Aleksandra, Vučković Opavski, Nataša, Milosavljević Tepavčević, Andreja, Ranin, Jovana, Gajić, Ina, "Biofilm Production and Antimicrobial Resistance of Clinical and Food Isolates of Pseudomonas spp." in Current Microbiology, 77 (2020):4045-4052,
https://doi.org/10.1007/s00284-020-02236-4 . .

TY  - CONF
AU  - Savić Radovanović, Radoslava
AU  - Rajić Savić, Nataša
AU  - Đorđević, Jasna
AU  - Babić, Milijana
AU  - Zdravković, Nemanja
AU  - Gajić, Ina
PY  - 2018
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2858
AB  - The genus Pseudomonas includes gram-negative bacilli, not requiring nutrient compounds necessary for growth and due to its relatively large genome and flexible metabolic capabilities, this organism exploits numerous environmental niches. They are often isolated from food, soil, manure and animal skin. It is an opportunistic pathogen that can cause acute, or chronic infections of various mammalian hosts and their organs due to the ability to form a wide spectrum of virulence factors.. Pseudomonas species grow well on bacterial media and often produce pigments, such as P. aeruginosa-thermostable blue-green pigment (pyocyanin) and P. flurescens-greenish-yellow pigment fluorescin. From the view point of milk hygiene the importance of this psychotrophic organism is, even it is destroyed by the pasteurization process, ability to synthesize thermostable metabolic products and enzymes that cause spoilage of milk products. As an infectious agent, can cause mastitis in a clinically expressed or subclinic form in cows. Usually they occur sporadically, but they can cause serious problems in the herd. Since, the ability to form biofilm presents a significant virulence factor for the establishment of persistent mammary gland infections, and a biofilm protects the bacterial community from antimicrobial drugs reducing the success of antimicrobial therapy, presenting a constant source of contamination, we decided to examine the biofilm abbility in Pseudomonas spp. from milk. A total of 59 isolates from bulk tank milk (<400.000 SCC/ml) were examened and identified by examining the phenotypic characteristics. Proteolitic and lipolitic activity was observed on Milk agar and Tributyrin agar. The Crystal Violet method was used to test the ability of the isolates to form biofilm. All 59 isolates, oxidase and catalase positive produced β hemolysis on blood agar, a pigment on Tripton soy agar (TSA) and had a distinctive smell. All 59 isolates showed proteolytic and lipolytic activity. Out of 59 isolates 57 were identified as Pseudomonas floroscens, and 2 isolates Pseudomonas aeriginosa produced green pigment-pyocyanin. The CV test extinctions showed that 22% isolates were strong producesrs 68% were intermediate, /% isolates were extremely strong biofilm producers, and  4% of isolates did not produce biofilmAlthough, the legislation in Republic of Serbia does not provide the examination of milk samples for the presence of Pseudomonas species, it is possible to find this organism in raw milk as a result of contamination. Monitoring the biofilm forming ability of Pseudomonas species may provide new ideas or strategy for the prevention or more efficient  treatment of bovine mastitis and battle in eliminating this source of contamination.
PB  - Novi Sad : University of Novi Sad, Institute of Food Technology
C3  - XVIII International Symposium Feed Technology, Novi Sad, 23 - 25. October 2018
T1  - Investigation of biofilm forming ability in Pseudomonas spp. from milk
SP  - 170
EP  - 171
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_2858
ER  - 

@conference{
author = "Savić Radovanović, Radoslava and Rajić Savić, Nataša and Đorđević, Jasna and Babić, Milijana and Zdravković, Nemanja and Gajić, Ina",
year = "2018",
abstract = "The genus Pseudomonas includes gram-negative bacilli, not requiring nutrient compounds necessary for growth and due to its relatively large genome and flexible metabolic capabilities, this organism exploits numerous environmental niches. They are often isolated from food, soil, manure and animal skin. It is an opportunistic pathogen that can cause acute, or chronic infections of various mammalian hosts and their organs due to the ability to form a wide spectrum of virulence factors.. Pseudomonas species grow well on bacterial media and often produce pigments, such as P. aeruginosa-thermostable blue-green pigment (pyocyanin) and P. flurescens-greenish-yellow pigment fluorescin. From the view point of milk hygiene the importance of this psychotrophic organism is, even it is destroyed by the pasteurization process, ability to synthesize thermostable metabolic products and enzymes that cause spoilage of milk products. As an infectious agent, can cause mastitis in a clinically expressed or subclinic form in cows. Usually they occur sporadically, but they can cause serious problems in the herd. Since, the ability to form biofilm presents a significant virulence factor for the establishment of persistent mammary gland infections, and a biofilm protects the bacterial community from antimicrobial drugs reducing the success of antimicrobial therapy, presenting a constant source of contamination, we decided to examine the biofilm abbility in Pseudomonas spp. from milk. A total of 59 isolates from bulk tank milk (<400.000 SCC/ml) were examened and identified by examining the phenotypic characteristics. Proteolitic and lipolitic activity was observed on Milk agar and Tributyrin agar. The Crystal Violet method was used to test the ability of the isolates to form biofilm. All 59 isolates, oxidase and catalase positive produced β hemolysis on blood agar, a pigment on Tripton soy agar (TSA) and had a distinctive smell. All 59 isolates showed proteolytic and lipolytic activity. Out of 59 isolates 57 were identified as Pseudomonas floroscens, and 2 isolates Pseudomonas aeriginosa produced green pigment-pyocyanin. The CV test extinctions showed that 22% isolates were strong producesrs 68% were intermediate, /% isolates were extremely strong biofilm producers, and  4% of isolates did not produce biofilmAlthough, the legislation in Republic of Serbia does not provide the examination of milk samples for the presence of Pseudomonas species, it is possible to find this organism in raw milk as a result of contamination. Monitoring the biofilm forming ability of Pseudomonas species may provide new ideas or strategy for the prevention or more efficient  treatment of bovine mastitis and battle in eliminating this source of contamination.",
publisher = "Novi Sad : University of Novi Sad, Institute of Food Technology",
journal = "XVIII International Symposium Feed Technology, Novi Sad, 23 - 25. October 2018",
title = "Investigation of biofilm forming ability in Pseudomonas spp. from milk",
pages = "170-171",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_2858"
}

Savić Radovanović, R., Rajić Savić, N., Đorđević, J., Babić, M., Zdravković, N.,& Gajić, I.. (2018). Investigation of biofilm forming ability in Pseudomonas spp. from milk. in XVIII International Symposium Feed Technology, Novi Sad, 23 - 25. October 2018
Novi Sad : University of Novi Sad, Institute of Food Technology., 170-171.
https://hdl.handle.net/21.15107/rcub_veterinar_2858

Savić Radovanović R, Rajić Savić N, Đorđević J, Babić M, Zdravković N, Gajić I. Investigation of biofilm forming ability in Pseudomonas spp. from milk. in XVIII International Symposium Feed Technology, Novi Sad, 23 - 25. October 2018. 2018;:170-171.
https://hdl.handle.net/21.15107/rcub_veterinar_2858 .

Savić Radovanović, Radoslava, Rajić Savić, Nataša, Đorđević, Jasna, Babić, Milijana, Zdravković, Nemanja, Gajić, Ina, "Investigation of biofilm forming ability in Pseudomonas spp. from milk" in XVIII International Symposium Feed Technology, Novi Sad, 23 - 25. October 2018 (2018):170-171,
https://hdl.handle.net/21.15107/rcub_veterinar_2858 .