TY  - JOUR
AU  - Beletić, Anđelo
AU  - Janjić, Filip
AU  - Radaković, Milena
AU  - Spariosu, Kristina
AU  - Francuski Andrić, Jelena
AU  - Chandrashekar, Ramaswamy
AU  - Tyrrell, Phyllis
AU  - Radonjić, Vladimir
AU  - Balint, Bela
AU  - Ajtić, Jelena
AU  - Kovačević-Filipović, Milica
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2015
AB  - The common signs of canine babesiosis caused by an infection with Babesia canis are fever, anorexia, lethargy, pulse alterations, anemia, and occasionally mild icterus. Dogs with these clinical signs can be divided into two groups: those with acute-phase reaction and those with systemic inflammatory response syndrome (SIRS). Factors associated with the occurrence of SIRS in canine babesiosis have not been thoroughly researched. This article outlines a cross-sectional study of 54 client-owned dogs with an acute B. canis infection, and evaluates the differences in age, gender, laboratory findings, parasite load, and seroreactivity against B. canis between the SIRS and the SIRS-free dogs. We have analyzed a complete blood count, serum biochemistry, serum amyloid A, ceruloplasmin, paraoxonase-1, serology, and PCR testing using standard methodologies. The frequency of SIRS among the investigated dogs reached 0.59. Male dogs and those seronegative against B. canis, were more frequent in the SIRS group, whilst age and parasite load could not be associated with the presence of SIRS. Dogs with SIRS had a lower count of total leukocytes, neutrophils, lymphocytes, and monocytes, and a lower concentration of iron and bilirubin compared with SIRS-free dogs. No significant differences in the concentration of acute-phase proteins have been noticed to exist between the groups of dogs. Further, the seronegative dogs had a lower count of lymphocytes and monocytes and a higher parasite load than the seroreactive dogs. Multivariate logistic regression analysis has identified leukopenia (<6 × 109/L) and monocytopenia (<0.2 × 109/L) as independent associates of SIRS in the investigated dogs, thus implying that these routine tests could be used as reliable markers for SIRS.
PB  - Elsevier
T2  - Veterinary Parasitology
T1  - Systemic inflammatory response syndrome in dogs naturally infected with Babesia canis: Association with the parasite load and host factors
VL  - 291
DO  - 10.1016/j.vetpar.2021.109366
ER  - 

@article{
author = "Beletić, Anđelo and Janjić, Filip and Radaković, Milena and Spariosu, Kristina and Francuski Andrić, Jelena and Chandrashekar, Ramaswamy and Tyrrell, Phyllis and Radonjić, Vladimir and Balint, Bela and Ajtić, Jelena and Kovačević-Filipović, Milica",
year = "2021",
abstract = "The common signs of canine babesiosis caused by an infection with Babesia canis are fever, anorexia, lethargy, pulse alterations, anemia, and occasionally mild icterus. Dogs with these clinical signs can be divided into two groups: those with acute-phase reaction and those with systemic inflammatory response syndrome (SIRS). Factors associated with the occurrence of SIRS in canine babesiosis have not been thoroughly researched. This article outlines a cross-sectional study of 54 client-owned dogs with an acute B. canis infection, and evaluates the differences in age, gender, laboratory findings, parasite load, and seroreactivity against B. canis between the SIRS and the SIRS-free dogs. We have analyzed a complete blood count, serum biochemistry, serum amyloid A, ceruloplasmin, paraoxonase-1, serology, and PCR testing using standard methodologies. The frequency of SIRS among the investigated dogs reached 0.59. Male dogs and those seronegative against B. canis, were more frequent in the SIRS group, whilst age and parasite load could not be associated with the presence of SIRS. Dogs with SIRS had a lower count of total leukocytes, neutrophils, lymphocytes, and monocytes, and a lower concentration of iron and bilirubin compared with SIRS-free dogs. No significant differences in the concentration of acute-phase proteins have been noticed to exist between the groups of dogs. Further, the seronegative dogs had a lower count of lymphocytes and monocytes and a higher parasite load than the seroreactive dogs. Multivariate logistic regression analysis has identified leukopenia (<6 × 109/L) and monocytopenia (<0.2 × 109/L) as independent associates of SIRS in the investigated dogs, thus implying that these routine tests could be used as reliable markers for SIRS.",
publisher = "Elsevier",
journal = "Veterinary Parasitology",
title = "Systemic inflammatory response syndrome in dogs naturally infected with Babesia canis: Association with the parasite load and host factors",
volume = "291",
doi = "10.1016/j.vetpar.2021.109366"
}

Beletić, A., Janjić, F., Radaković, M., Spariosu, K., Francuski Andrić, J., Chandrashekar, R., Tyrrell, P., Radonjić, V., Balint, B., Ajtić, J.,& Kovačević-Filipović, M.. (2021). Systemic inflammatory response syndrome in dogs naturally infected with Babesia canis: Association with the parasite load and host factors. in Veterinary Parasitology
Elsevier., 291.
https://doi.org/10.1016/j.vetpar.2021.109366

Beletić A, Janjić F, Radaković M, Spariosu K, Francuski Andrić J, Chandrashekar R, Tyrrell P, Radonjić V, Balint B, Ajtić J, Kovačević-Filipović M. Systemic inflammatory response syndrome in dogs naturally infected with Babesia canis: Association with the parasite load and host factors. in Veterinary Parasitology. 2021;291.
doi:10.1016/j.vetpar.2021.109366 .

Beletić, Anđelo, Janjić, Filip, Radaković, Milena, Spariosu, Kristina, Francuski Andrić, Jelena, Chandrashekar, Ramaswamy, Tyrrell, Phyllis, Radonjić, Vladimir, Balint, Bela, Ajtić, Jelena, Kovačević-Filipović, Milica, "Systemic inflammatory response syndrome in dogs naturally infected with Babesia canis: Association with the parasite load and host factors" in Veterinary Parasitology, 291 (2021),
https://doi.org/10.1016/j.vetpar.2021.109366 . .

TY  - JOUR
AU  - Ilić Božović, Anja
AU  - Radaković, Milena
AU  - Spariosu, Kristina
AU  - Tyrrell, Phyllis
AU  - Chandrashekar, Ramaswamy
AU  - Mišić, Dušan
AU  - Kovačević-Filipović, Milica
PY  - 2021
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2043
AB  - In September 2018, a four-month-old dog with fever and petechial bleeding came to the internal clinic at the Faculty of Veterinary Medicine University of Belgrade. On hematology analysis, thrombocytopenia and mild anemia were observed. Examination of the blood smear revealed platelet inclusions. The commercial serology test was positive for Anaplasma spp. The dog was treated with doxycycline for 14 days, and after 48 hours from the beginning of the treatment, the symptoms subsided. PCR analysis and sequencing confirmed infection with A. platys.
AB  - U septembru 2018. godine, štene staro četiri meseca, sa znacima groznice i petehijalnim krvarenjem, je došlo u ambulantu Klinike za male životinje Fakulteta veterinarske medicine Univerziteta u Beogradu. Hematološka analiza je pokazala trombocitopeniju i blagu anemiju. Pregledom krvnog razmaza ustanovljene su inkluzije u trombocitima. Komercijalni serološki test je pokazao prisustvo antitela protiv Anaplasma spp. Sprovedena je terapija doksiciklinom u trajanju od 14 dana, a posle 48 sati od početka terapije znaci bolesti su se povukli. PCR analizom i senkvencioniranjem je potvrđeno prisustvo Anaplasma platys.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - First confirmed clinical case of Anaplasma platys in a dog in Serbia
T1  - Prvi potvrđeni klinički slučaj infekcije psa vrstom Anaplasma platys u Srbiji
VL  - 71
IS  - 1
SP  - 107
EP  - 112
DO  - 10.2478/acve-2021-0008
ER  - 

@article{
author = "Ilić Božović, Anja and Radaković, Milena and Spariosu, Kristina and Tyrrell, Phyllis and Chandrashekar, Ramaswamy and Mišić, Dušan and Kovačević-Filipović, Milica",
year = "2021",
abstract = "In September 2018, a four-month-old dog with fever and petechial bleeding came to the internal clinic at the Faculty of Veterinary Medicine University of Belgrade. On hematology analysis, thrombocytopenia and mild anemia were observed. Examination of the blood smear revealed platelet inclusions. The commercial serology test was positive for Anaplasma spp. The dog was treated with doxycycline for 14 days, and after 48 hours from the beginning of the treatment, the symptoms subsided. PCR analysis and sequencing confirmed infection with A. platys., U septembru 2018. godine, štene staro četiri meseca, sa znacima groznice i petehijalnim krvarenjem, je došlo u ambulantu Klinike za male životinje Fakulteta veterinarske medicine Univerziteta u Beogradu. Hematološka analiza je pokazala trombocitopeniju i blagu anemiju. Pregledom krvnog razmaza ustanovljene su inkluzije u trombocitima. Komercijalni serološki test je pokazao prisustvo antitela protiv Anaplasma spp. Sprovedena je terapija doksiciklinom u trajanju od 14 dana, a posle 48 sati od početka terapije znaci bolesti su se povukli. PCR analizom i senkvencioniranjem je potvrđeno prisustvo Anaplasma platys.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "First confirmed clinical case of Anaplasma platys in a dog in Serbia, Prvi potvrđeni klinički slučaj infekcije psa vrstom Anaplasma platys u Srbiji",
volume = "71",
number = "1",
pages = "107-112",
doi = "10.2478/acve-2021-0008"
}

Ilić Božović, A., Radaković, M., Spariosu, K., Tyrrell, P., Chandrashekar, R., Mišić, D.,& Kovačević-Filipović, M.. (2021). First confirmed clinical case of Anaplasma platys in a dog in Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 71(1), 107-112.
https://doi.org/10.2478/acve-2021-0008

Ilić Božović A, Radaković M, Spariosu K, Tyrrell P, Chandrashekar R, Mišić D, Kovačević-Filipović M. First confirmed clinical case of Anaplasma platys in a dog in Serbia. in Acta Veterinaria-Beograd. 2021;71(1):107-112.
doi:10.2478/acve-2021-0008 .

Ilić Božović, Anja, Radaković, Milena, Spariosu, Kristina, Tyrrell, Phyllis, Chandrashekar, Ramaswamy, Mišić, Dušan, Kovačević-Filipović, Milica, "First confirmed clinical case of Anaplasma platys in a dog in Serbia" in Acta Veterinaria-Beograd, 71, no. 1 (2021):107-112,
https://doi.org/10.2478/acve-2021-0008 . .

TY  - JOUR
AU  - Milanović, Zorana
AU  - Vekić, Jelena
AU  - Radonjić, Vladimir
AU  - Božović-Ilić, Anja
AU  - Zeljković, Aleksandra
AU  - Janac, Jelena
AU  - Spasojevic-Kalimanovska, Vesna
AU  - Buch, Jesse
AU  - Chandrashekar, Ramaswamy
AU  - Bojic-Trbojević, Zanka
AU  - Hajduković, Ljiljana
AU  - Christopher, Mary M.
AU  - Kovačević-Filipović, Milica
PY  - 2019
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1778
AB  - Background Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results Dogs with B. canis infection had a marked APR (median SAA, 168.3 mu g/mL; range, 98.1-716.2 mu g/mL) compared with controls (3.2 mu g/mL, 2.0-4.2 mu g/mL) (P < .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P = .02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P = .02), and alpha-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P = .04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P = .02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho = .43; P = .03) and ApoA-1 (rho = .63, P < .001). Conclusions and Clinical Importance Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.
PB  - Wiley, Hoboken
T2  - Journal of Veterinary Internal Medicine
T1  - Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs
VL  - 33
IS  - 4
SP  - 1686
EP  - 1694
DO  - 10.1111/jvim.15537
ER  - 

@article{
author = "Milanović, Zorana and Vekić, Jelena and Radonjić, Vladimir and Božović-Ilić, Anja and Zeljković, Aleksandra and Janac, Jelena and Spasojevic-Kalimanovska, Vesna and Buch, Jesse and Chandrashekar, Ramaswamy and Bojic-Trbojević, Zanka and Hajduković, Ljiljana and Christopher, Mary M. and Kovačević-Filipović, Milica",
year = "2019",
abstract = "Background Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results Dogs with B. canis infection had a marked APR (median SAA, 168.3 mu g/mL; range, 98.1-716.2 mu g/mL) compared with controls (3.2 mu g/mL, 2.0-4.2 mu g/mL) (P < .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P = .02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P = .02), and alpha-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P = .04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P = .02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho = .43; P = .03) and ApoA-1 (rho = .63, P < .001). Conclusions and Clinical Importance Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.",
publisher = "Wiley, Hoboken",
journal = "Journal of Veterinary Internal Medicine",
title = "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs",
volume = "33",
number = "4",
pages = "1686-1694",
doi = "10.1111/jvim.15537"
}

Milanović, Z., Vekić, J., Radonjić, V., Božović-Ilić, A., Zeljković, A., Janac, J., Spasojevic-Kalimanovska, V., Buch, J., Chandrashekar, R., Bojic-Trbojević, Z., Hajduković, L., Christopher, M. M.,& Kovačević-Filipović, M.. (2019). Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine
Wiley, Hoboken., 33(4), 1686-1694.
https://doi.org/10.1111/jvim.15537

Milanović Z, Vekić J, Radonjić V, Božović-Ilić A, Zeljković A, Janac J, Spasojevic-Kalimanovska V, Buch J, Chandrashekar R, Bojic-Trbojević Z, Hajduković L, Christopher MM, Kovačević-Filipović M. Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine. 2019;33(4):1686-1694.
doi:10.1111/jvim.15537 .

Milanović, Zorana, Vekić, Jelena, Radonjić, Vladimir, Božović-Ilić, Anja, Zeljković, Aleksandra, Janac, Jelena, Spasojevic-Kalimanovska, Vesna, Buch, Jesse, Chandrashekar, Ramaswamy, Bojic-Trbojević, Zanka, Hajduković, Ljiljana, Christopher, Mary M., Kovačević-Filipović, Milica, "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs" in Journal of Veterinary Internal Medicine, 33, no. 4 (2019):1686-1694,
https://doi.org/10.1111/jvim.15537 . .