TY  - JOUR
AU  - Đurđević, S.
AU  - Jezdimirović, Milanka
AU  - Aleksić, Nevenka
AU  - Branković, Nataša
AU  - Pržulj, D.
AU  - Cicović, B.
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/684
AB  - Concentrations of the NSAID metamizol sodium (MmNa) in the synovial fluid and hyaline cartilage of inflamed knee and elbow joints of pigs after i.v. application and iontophoresis (IPh) were investigated. The research was conducted on 14 male pigs divided equally into two experimental groups and exposed to artificial inflammation of knees and elbows prior to the application of MmNa. The first group (A) was administered 2.5 g (twice the maximum therapeutic dose) MmNa intravenously, whilst the second group (B) was exposed to the same dose, but applied by IPh into contralateral knee and elbow joints. Four hours after the application of MmNa biopsies of the affected knee and elbow joints were performed. The average concentration of MmNa in the synovial fluid of inflamed joints in group A was 9.81±1.96μg/g, while in group B was 170.66±2.07 μg/g, being 17 times higher. The average concentration of MmNa in the hyaline cartilage was 2.29±1.16μg/g following i.v. administration and 98.36±21.58μg/g after IPh, i.e. approximatelly 43 times higher. This led to the conclusion that IPh of MmNa, resulting in incomparably higher concentrations in inflamed joints without any adverse systemic effects, has an advantage over i.v. application.
AB  - U sinovijalnoj tečnosti i hijalinoj hrskavici inflamiranog kolenog i lakatnog zgloba svinja određivane su koncentracije metamizol-natrijuma (MmNa) aplikovanog i.v. i putem elektroforeze. Arteficijelno zapaljenje kolena i lakta prasadi izazvano je terpentinskim uljem. Metamizol-natrijum je primenjen u dozi od 2.5 g u prvoj grupi prasadi intravenski, a u drugoj putem elektroforeze u kontalateralni koleni i lakatni zglob. Četiri sata posle aplikacije MmNa urađena je biopsija tretiranih zglobova. Prosečna koncentracija MmNa u sinovijalnoj tečnosti inflamiranih zglobova prasadi posle i.v. aplikacije iznosila je 9,81±1,96μg/g, a posle elektroforeze bila je 170,66±2,07μg/g tkiva, odnosno oko 17 puta viša. Izmerena prosečna koncentracija MmNa u hijalinoj hrskavici iznosila je 2,29±1,16μg/g posle i.v. aplikacije i 98,36±21,58μg/g posle aplikacije elektroforezom, odnosno oko 43 puta više. Zaključujeno je da MmNa primenjen elektroforezom postiže statistički veoma značajno više prosečne koncentracije u inflamiranim zglobovima u odnosu na i.v. aplikaciju. Primena MmNa elektroforezom ima značajnu prednost nad i.v. aplikacijom ovog leka ne samo zbog postizanja značajno viših koncentracija u inflamiranim zglobovima, već i zbog izostajanja sistemskih neželjenih reakcija.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The determination of the concentrations of metamizol sodium in inflamed joints of pigs after intravenous and iontophoretic application
T1  - Određivanje koncentracija metamizol-natrijuma u inflamiranim zglobovima svinja posle intravenske i elektroforetske aplikacije
VL  - 60
IS  - 4
SP  - 371
EP  - 379
DO  - 10.2298/AVB1004371D
ER  - 

@article{
author = "Đurđević, S. and Jezdimirović, Milanka and Aleksić, Nevenka and Branković, Nataša and Pržulj, D. and Cicović, B.",
year = "2010",
abstract = "Concentrations of the NSAID metamizol sodium (MmNa) in the synovial fluid and hyaline cartilage of inflamed knee and elbow joints of pigs after i.v. application and iontophoresis (IPh) were investigated. The research was conducted on 14 male pigs divided equally into two experimental groups and exposed to artificial inflammation of knees and elbows prior to the application of MmNa. The first group (A) was administered 2.5 g (twice the maximum therapeutic dose) MmNa intravenously, whilst the second group (B) was exposed to the same dose, but applied by IPh into contralateral knee and elbow joints. Four hours after the application of MmNa biopsies of the affected knee and elbow joints were performed. The average concentration of MmNa in the synovial fluid of inflamed joints in group A was 9.81±1.96μg/g, while in group B was 170.66±2.07 μg/g, being 17 times higher. The average concentration of MmNa in the hyaline cartilage was 2.29±1.16μg/g following i.v. administration and 98.36±21.58μg/g after IPh, i.e. approximatelly 43 times higher. This led to the conclusion that IPh of MmNa, resulting in incomparably higher concentrations in inflamed joints without any adverse systemic effects, has an advantage over i.v. application., U sinovijalnoj tečnosti i hijalinoj hrskavici inflamiranog kolenog i lakatnog zgloba svinja određivane su koncentracije metamizol-natrijuma (MmNa) aplikovanog i.v. i putem elektroforeze. Arteficijelno zapaljenje kolena i lakta prasadi izazvano je terpentinskim uljem. Metamizol-natrijum je primenjen u dozi od 2.5 g u prvoj grupi prasadi intravenski, a u drugoj putem elektroforeze u kontalateralni koleni i lakatni zglob. Četiri sata posle aplikacije MmNa urađena je biopsija tretiranih zglobova. Prosečna koncentracija MmNa u sinovijalnoj tečnosti inflamiranih zglobova prasadi posle i.v. aplikacije iznosila je 9,81±1,96μg/g, a posle elektroforeze bila je 170,66±2,07μg/g tkiva, odnosno oko 17 puta viša. Izmerena prosečna koncentracija MmNa u hijalinoj hrskavici iznosila je 2,29±1,16μg/g posle i.v. aplikacije i 98,36±21,58μg/g posle aplikacije elektroforezom, odnosno oko 43 puta više. Zaključujeno je da MmNa primenjen elektroforezom postiže statistički veoma značajno više prosečne koncentracije u inflamiranim zglobovima u odnosu na i.v. aplikaciju. Primena MmNa elektroforezom ima značajnu prednost nad i.v. aplikacijom ovog leka ne samo zbog postizanja značajno viših koncentracija u inflamiranim zglobovima, već i zbog izostajanja sistemskih neželjenih reakcija.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The determination of the concentrations of metamizol sodium in inflamed joints of pigs after intravenous and iontophoretic application, Određivanje koncentracija metamizol-natrijuma u inflamiranim zglobovima svinja posle intravenske i elektroforetske aplikacije",
volume = "60",
number = "4",
pages = "371-379",
doi = "10.2298/AVB1004371D"
}

Đurđević, S., Jezdimirović, M., Aleksić, N., Branković, N., Pržulj, D.,& Cicović, B.. (2010). The determination of the concentrations of metamizol sodium in inflamed joints of pigs after intravenous and iontophoretic application. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(4), 371-379.
https://doi.org/10.2298/AVB1004371D

Đurđević S, Jezdimirović M, Aleksić N, Branković N, Pržulj D, Cicović B. The determination of the concentrations of metamizol sodium in inflamed joints of pigs after intravenous and iontophoretic application. in Acta Veterinaria-Beograd. 2010;60(4):371-379.
doi:10.2298/AVB1004371D .

Đurđević, S., Jezdimirović, Milanka, Aleksić, Nevenka, Branković, Nataša, Pržulj, D., Cicović, B., "The determination of the concentrations of metamizol sodium in inflamed joints of pigs after intravenous and iontophoretic application" in Acta Veterinaria-Beograd, 60, no. 4 (2010):371-379,
https://doi.org/10.2298/AVB1004371D . .

TY  - JOUR
AU  - Đurđević, S.
AU  - Jezdimirović, Milanka
AU  - Đurović, A.
AU  - Dedić, Gordana
AU  - Aleksić, Nevenka
AU  - Branković, Nataša
AU  - Stojiljković, S.
AU  - Stojšić, D.
AU  - Blagojević, Z.
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/569
AB  - The current clinical research on pharmacokinetics of methylprednisolone was performed on male pigs to whom was administered either intravenously or locally, via iontophoresis. Equal doses of methylprednisolone sodium succinate (MPSS) were applied, i.e. 40 mg it total per animal. In all pigs artificial inflammation of knee and elbow joints was provoked four days prior to the treatment. Four hours after the application of methylprednisolone tissue samples (both synovial fluid and hyaline cartilage) were obtained from the inflamed joints and subjected to analysis. The quantification of the drug was performed by HPLC technique. The results indicated high quantities of methylprednisolone both in the synovial fluid and hyaline cartilage, the concentrations being significantly higher in animals after iontophoretic application (17.15±3.11 and 12.70±2.19 μg/g, respectively) in comparison with the animals treated intravenously (0.33±0.11 and 0.21±0.06 μg/g, respectively). Thus, iontophoresis was proved a highly advisable clinical means of application of methylprednisolone, especially having in mind the possibility of avoiding systemic adverse effects which are present after parenteral drug administration. In addition, it enables higher therapeutic concentrations of MPSS to be obtained both in the synovial fluid and in the hyaline cartilage of the treated inflamed joints.
AB  - Ispitivana je farmakokinetika metil-prednizolona na svinjama muškog pola kojima je ovaj lek aplikovan intravenski ili lokalno, putem elektroforeze. Metilprednizolon-natrijum-sukcinat primenjen je jednokratno, u dozi od 40 mg po životinji. Četiri dana pre tretmana, kod svih svinja je izazvano arteficijelno zapaljenje kolenog i lakatnog zgloba. Četiri sata posle aplikacije metil-prednizolona uzorci tkiva (sinovijalna tečnost i hijalina hrskavica) uzeti su iz inflamiranih zglobova i zatim analizirani. Kvantifikacija metil-prednizolona obavljena je HPLC metodom. Rezultati su ukazali na prisustvo visokih koncentracija metil-prednizolona u sinovijalnoj tečnosti i hijalinoj hrskavici, koje su bile statistički signifikantno veće posle elektroforetske aplikacije (17,15 ± 3,11 u sinovijalnoj tečnosti i 12,70 ± 2,19 μg/g u hrskavici) u poređenju sa koncentracijama dobijenim posle intravenske primene (0,33 ± 0,11 u sinovijalnoj tečnosti i 0,21 ± 0,06 μg/g u hrskavičnom tkivu). Iz ovog razloga, elektroforeza se preporučuje kao način lokalne aplikacije metilprednizolona kod inflamacije zglobova, naročito kada se ima u vidu mogućnost da se time izbegavaju sistemski neželjeni efekti koji nastaju posle parenteralne primene. Elektroforeza obezbeđuje vrlo visoke terapijske koncentracije metilprednizolon-natrijum-sukcinata kako u sinovijalnoj tečnosti, tako i u hrskavici tretiranih inflamiranih zglobova svinja.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig
T1  - Evaluacija koncentracije metil-prednizolona posle intravenske i elektroforetske aplikacije kod domaće svinje
VL  - 59
IS  - 2-3
SP  - 157
EP  - 165
DO  - 10.2298/AVB0903157D
ER  - 

@article{
author = "Đurđević, S. and Jezdimirović, Milanka and Đurović, A. and Dedić, Gordana and Aleksić, Nevenka and Branković, Nataša and Stojiljković, S. and Stojšić, D. and Blagojević, Z.",
year = "2009",
abstract = "The current clinical research on pharmacokinetics of methylprednisolone was performed on male pigs to whom was administered either intravenously or locally, via iontophoresis. Equal doses of methylprednisolone sodium succinate (MPSS) were applied, i.e. 40 mg it total per animal. In all pigs artificial inflammation of knee and elbow joints was provoked four days prior to the treatment. Four hours after the application of methylprednisolone tissue samples (both synovial fluid and hyaline cartilage) were obtained from the inflamed joints and subjected to analysis. The quantification of the drug was performed by HPLC technique. The results indicated high quantities of methylprednisolone both in the synovial fluid and hyaline cartilage, the concentrations being significantly higher in animals after iontophoretic application (17.15±3.11 and 12.70±2.19 μg/g, respectively) in comparison with the animals treated intravenously (0.33±0.11 and 0.21±0.06 μg/g, respectively). Thus, iontophoresis was proved a highly advisable clinical means of application of methylprednisolone, especially having in mind the possibility of avoiding systemic adverse effects which are present after parenteral drug administration. In addition, it enables higher therapeutic concentrations of MPSS to be obtained both in the synovial fluid and in the hyaline cartilage of the treated inflamed joints., Ispitivana je farmakokinetika metil-prednizolona na svinjama muškog pola kojima je ovaj lek aplikovan intravenski ili lokalno, putem elektroforeze. Metilprednizolon-natrijum-sukcinat primenjen je jednokratno, u dozi od 40 mg po životinji. Četiri dana pre tretmana, kod svih svinja je izazvano arteficijelno zapaljenje kolenog i lakatnog zgloba. Četiri sata posle aplikacije metil-prednizolona uzorci tkiva (sinovijalna tečnost i hijalina hrskavica) uzeti su iz inflamiranih zglobova i zatim analizirani. Kvantifikacija metil-prednizolona obavljena je HPLC metodom. Rezultati su ukazali na prisustvo visokih koncentracija metil-prednizolona u sinovijalnoj tečnosti i hijalinoj hrskavici, koje su bile statistički signifikantno veće posle elektroforetske aplikacije (17,15 ± 3,11 u sinovijalnoj tečnosti i 12,70 ± 2,19 μg/g u hrskavici) u poređenju sa koncentracijama dobijenim posle intravenske primene (0,33 ± 0,11 u sinovijalnoj tečnosti i 0,21 ± 0,06 μg/g u hrskavičnom tkivu). Iz ovog razloga, elektroforeza se preporučuje kao način lokalne aplikacije metilprednizolona kod inflamacije zglobova, naročito kada se ima u vidu mogućnost da se time izbegavaju sistemski neželjeni efekti koji nastaju posle parenteralne primene. Elektroforeza obezbeđuje vrlo visoke terapijske koncentracije metilprednizolon-natrijum-sukcinata kako u sinovijalnoj tečnosti, tako i u hrskavici tretiranih inflamiranih zglobova svinja.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig, Evaluacija koncentracije metil-prednizolona posle intravenske i elektroforetske aplikacije kod domaće svinje",
volume = "59",
number = "2-3",
pages = "157-165",
doi = "10.2298/AVB0903157D"
}

Đurđević, S., Jezdimirović, M., Đurović, A., Dedić, G., Aleksić, N., Branković, N., Stojiljković, S., Stojšić, D.,& Blagojević, Z.. (2009). The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(2-3), 157-165.
https://doi.org/10.2298/AVB0903157D

Đurđević S, Jezdimirović M, Đurović A, Dedić G, Aleksić N, Branković N, Stojiljković S, Stojšić D, Blagojević Z. The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig. in Acta Veterinaria-Beograd. 2009;59(2-3):157-165.
doi:10.2298/AVB0903157D .

Đurđević, S., Jezdimirović, Milanka, Đurović, A., Dedić, Gordana, Aleksić, Nevenka, Branković, Nataša, Stojiljković, S., Stojšić, D., Blagojević, Z., "The evaluation of the concentrations of methylprednisolone applied intravenously and by iontophoresis in the pig" in Acta Veterinaria-Beograd, 59, no. 2-3 (2009):157-165,
https://doi.org/10.2298/AVB0903157D . .

TY  - JOUR
AU  - Ivanović, Saša
AU  - Borozan, Sunčica
AU  - Jezdimirović, Milanka
AU  - Aleksić, Nevenka
AU  - Milovanović, Mirjana
AU  - Tomašević-Čanović, Magdalena
AU  - Đurđević, S.
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/466
AB  - The protective effect of bentonite (natural and synthetic) in oxidative stress induced by paraquat was studied on 32 adult male Wistar rats. The animals were divided into four groups (n=8). The first group received only paraquat p.o. (via a gastric tube). An hour after administration of paraquat, the second and the third group were treated with natural and synthetic bentonite, respectively, while the fourth group was untreated (control). All three experimental groups were treated once a day, throughout 8 consecutive days. Blood samples were taken 0, 1, 4, 6 and 8 days after the beginning of the treatment. Oxidative stress induced by paraquat was estimated by means of catalase activity (CAT - mmol/H2O2/min/g Hb) and malondialdehyde quantity (MDA - nmol/g Hb). Oxidative stress proved to be significant an hour and 192 hours after the beginning of chronic intoxication by paraquat. Both natural and synthetic bentonite expressed a protective action from oxidative stress in the period from 24 h to 144 h post application, acting through the external capacity of cationic exchange, which is known to be approximately 10%. The absorption of paraquat depends on the size of the molecule and its polarisation and is performed by the mechanisms of ion exchange (ionic and electrostatic interaction). Nevertheless, both absorbents developed a significant protective effect (approximately 50 %) 1 h and 192 h after the first application of paraquat, indicating that these protectors act as molecular sieves and thus suppress the process of lipid peroxidation and the development of even more intensive oxidative stress.
AB  - U ovom radu su izneti rezultati proučavanja protektivnog efekta prirodnog i sintetskog bentonita tokom oksidativnog stresa indukovanog aplikacijom parahvata pacovima soja Wistar. Ogledne životinje su bile podeljene u četiri jednake grupe. Prva grupa pacova tretirana je samo parahvatom pomoću želudačne sonde. Jedan sat nakon aplikacije parahvata, pacovi druge i treće grupe su bili tretirani prirodnim ili sintetskim bentonitom, aplikovanim per os. Četvrta grupa pacova nije tretirana i služila je kao negativna kontrola. Eksperimentalne grupe životinja su bile tretirane jednom dnevno tokom osam uzastopnih dana. Uzorci krvi su uzimani 0, 1, 4, 6 i 8 dana nakon prvog tretmana. Stepen oksidativnog stresa je procenjivan na osnovu aktivnosti enzima katalaze i na osnovu količine malondialdehida. Prirodni i sintetski bentonit ispoljili su protektivnu aktivnost smanjivanjem parametara oksidativnog stresa u periodu od 24 do 144 sata nakon aplikacije. Autori predpostavljaju da bentonit deluje kao molekularno sito suprimirajući proces lipidne peroksidacije.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The application of adsorbent bentonite in oxidative stress induced by paraquat
T1  - Primena bentonita kao adsorbenta tokom oksidativnog stresa indukovanog parahvatom
VL  - 57
IS  - 4
SP  - 329
EP  - 340
DO  - 10.2298/AVB0704329I
ER  - 

@article{
author = "Ivanović, Saša and Borozan, Sunčica and Jezdimirović, Milanka and Aleksić, Nevenka and Milovanović, Mirjana and Tomašević-Čanović, Magdalena and Đurđević, S.",
year = "2007",
abstract = "The protective effect of bentonite (natural and synthetic) in oxidative stress induced by paraquat was studied on 32 adult male Wistar rats. The animals were divided into four groups (n=8). The first group received only paraquat p.o. (via a gastric tube). An hour after administration of paraquat, the second and the third group were treated with natural and synthetic bentonite, respectively, while the fourth group was untreated (control). All three experimental groups were treated once a day, throughout 8 consecutive days. Blood samples were taken 0, 1, 4, 6 and 8 days after the beginning of the treatment. Oxidative stress induced by paraquat was estimated by means of catalase activity (CAT - mmol/H2O2/min/g Hb) and malondialdehyde quantity (MDA - nmol/g Hb). Oxidative stress proved to be significant an hour and 192 hours after the beginning of chronic intoxication by paraquat. Both natural and synthetic bentonite expressed a protective action from oxidative stress in the period from 24 h to 144 h post application, acting through the external capacity of cationic exchange, which is known to be approximately 10%. The absorption of paraquat depends on the size of the molecule and its polarisation and is performed by the mechanisms of ion exchange (ionic and electrostatic interaction). Nevertheless, both absorbents developed a significant protective effect (approximately 50 %) 1 h and 192 h after the first application of paraquat, indicating that these protectors act as molecular sieves and thus suppress the process of lipid peroxidation and the development of even more intensive oxidative stress., U ovom radu su izneti rezultati proučavanja protektivnog efekta prirodnog i sintetskog bentonita tokom oksidativnog stresa indukovanog aplikacijom parahvata pacovima soja Wistar. Ogledne životinje su bile podeljene u četiri jednake grupe. Prva grupa pacova tretirana je samo parahvatom pomoću želudačne sonde. Jedan sat nakon aplikacije parahvata, pacovi druge i treće grupe su bili tretirani prirodnim ili sintetskim bentonitom, aplikovanim per os. Četvrta grupa pacova nije tretirana i služila je kao negativna kontrola. Eksperimentalne grupe životinja su bile tretirane jednom dnevno tokom osam uzastopnih dana. Uzorci krvi su uzimani 0, 1, 4, 6 i 8 dana nakon prvog tretmana. Stepen oksidativnog stresa je procenjivan na osnovu aktivnosti enzima katalaze i na osnovu količine malondialdehida. Prirodni i sintetski bentonit ispoljili su protektivnu aktivnost smanjivanjem parametara oksidativnog stresa u periodu od 24 do 144 sata nakon aplikacije. Autori predpostavljaju da bentonit deluje kao molekularno sito suprimirajući proces lipidne peroksidacije.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The application of adsorbent bentonite in oxidative stress induced by paraquat, Primena bentonita kao adsorbenta tokom oksidativnog stresa indukovanog parahvatom",
volume = "57",
number = "4",
pages = "329-340",
doi = "10.2298/AVB0704329I"
}

Ivanović, S., Borozan, S., Jezdimirović, M., Aleksić, N., Milovanović, M., Tomašević-Čanović, M.,& Đurđević, S.. (2007). The application of adsorbent bentonite in oxidative stress induced by paraquat. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(4), 329-340.
https://doi.org/10.2298/AVB0704329I

Ivanović S, Borozan S, Jezdimirović M, Aleksić N, Milovanović M, Tomašević-Čanović M, Đurđević S. The application of adsorbent bentonite in oxidative stress induced by paraquat. in Acta Veterinaria-Beograd. 2007;57(4):329-340.
doi:10.2298/AVB0704329I .

Ivanović, Saša, Borozan, Sunčica, Jezdimirović, Milanka, Aleksić, Nevenka, Milovanović, Mirjana, Tomašević-Čanović, Magdalena, Đurđević, S., "The application of adsorbent bentonite in oxidative stress induced by paraquat" in Acta Veterinaria-Beograd, 57, no. 4 (2007):329-340,
https://doi.org/10.2298/AVB0704329I . .