TY  - JOUR
AU  - Betić, Nikola
AU  - Karabasil, Nedjeljko
AU  - Djurković-Djaković, Olgica
AU  - Ćirković, Vladimir
AU  - Bobić, Branko
AU  - Branković Lazić, Ivana
AU  - Djordjević, Vesna
AU  - Klun, Ivana
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2464
AB  - Consumption of Toxoplasma gondii contaminated pork is a major risk factor for human infection. We thus conducted a cross-sectional survey on the seroprevalence of T. gondii infection in a representative sample of slaughter pigs from throughout Serbia and examined the influence of farm biosecurity-related risk factors on infection. In addition, direct detection of the parasite (by mouse bioassay) or its DNA was performed in the hearts of a subset of seropositive sows. The overall seroprevalence in the sample of 825 pigs as determined by the modified agglutination test (MAT) was 16.5%. Older age and inadequate rodent control were independent infection risk factors for pigs. In a subset of 581 pigs with complete biosecurity-related data, in addition to older age, smallholders’ finishing type farms (as opposed to farrow-to-finish), multispecies farming, and origin from Western and Central and South-Eastern Serbia (vs. the Northern region), all increased the risk of infection, while the absence of disinfection boot-dips in front of each barn and Belgrade district origin (vs. the Northern region) were associated with a 62% and 75% lower risk of infection, respectively. Evidence of viable parasites was obtained in 13 (41.9%) of the 31 bioassayed sow hearts, of which by isolation of brain cysts in seven, by detection of T. gondii DNA in an additional four, and by serology in another two. Recovery of brain cysts mostly (5/7) from sows with a MAT titre of ≥1:100 indicates the risk for consumers. These results highlight the public health risk from pork consumption and point to mandatory use of professional rodent control services, abstaining from multispecies farming, keeping disinfection boot-dips clean and freshly refilled, as well as strict implementation of zoo-hygienic measures on smallholders’ farms as specific farm biosecurity measures needed for its reduction.
PB  - MDPI
T2  - Microorganisms
T1  - Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures
VL  - 10
IS  - 5
SP  - 1069
DO  - 10.3390/microorganisms10051069
ER  - 

@article{
author = "Betić, Nikola and Karabasil, Nedjeljko and Djurković-Djaković, Olgica and Ćirković, Vladimir and Bobić, Branko and Branković Lazić, Ivana and Djordjević, Vesna and Klun, Ivana",
year = "2022",
abstract = "Consumption of Toxoplasma gondii contaminated pork is a major risk factor for human infection. We thus conducted a cross-sectional survey on the seroprevalence of T. gondii infection in a representative sample of slaughter pigs from throughout Serbia and examined the influence of farm biosecurity-related risk factors on infection. In addition, direct detection of the parasite (by mouse bioassay) or its DNA was performed in the hearts of a subset of seropositive sows. The overall seroprevalence in the sample of 825 pigs as determined by the modified agglutination test (MAT) was 16.5%. Older age and inadequate rodent control were independent infection risk factors for pigs. In a subset of 581 pigs with complete biosecurity-related data, in addition to older age, smallholders’ finishing type farms (as opposed to farrow-to-finish), multispecies farming, and origin from Western and Central and South-Eastern Serbia (vs. the Northern region), all increased the risk of infection, while the absence of disinfection boot-dips in front of each barn and Belgrade district origin (vs. the Northern region) were associated with a 62% and 75% lower risk of infection, respectively. Evidence of viable parasites was obtained in 13 (41.9%) of the 31 bioassayed sow hearts, of which by isolation of brain cysts in seven, by detection of T. gondii DNA in an additional four, and by serology in another two. Recovery of brain cysts mostly (5/7) from sows with a MAT titre of ≥1:100 indicates the risk for consumers. These results highlight the public health risk from pork consumption and point to mandatory use of professional rodent control services, abstaining from multispecies farming, keeping disinfection boot-dips clean and freshly refilled, as well as strict implementation of zoo-hygienic measures on smallholders’ farms as specific farm biosecurity measures needed for its reduction.",
publisher = "MDPI",
journal = "Microorganisms",
title = "Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures",
volume = "10",
number = "5",
pages = "1069",
doi = "10.3390/microorganisms10051069"
}

Betić, N., Karabasil, N., Djurković-Djaković, O., Ćirković, V., Bobić, B., Branković Lazić, I., Djordjević, V.,& Klun, I.. (2022). Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures. in Microorganisms
MDPI., 10(5), 1069.
https://doi.org/10.3390/microorganisms10051069

Betić N, Karabasil N, Djurković-Djaković O, Ćirković V, Bobić B, Branković Lazić I, Djordjević V, Klun I. Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures. in Microorganisms. 2022;10(5):1069.
doi:10.3390/microorganisms10051069 .

Betić, Nikola, Karabasil, Nedjeljko, Djurković-Djaković, Olgica, Ćirković, Vladimir, Bobić, Branko, Branković Lazić, Ivana, Djordjević, Vesna, Klun, Ivana, "Seroprevalence, Direct Detection and Risk Factors for Toxoplasma gondii Infection in Pigs in Serbia, and Influence of Biosecurity Measures" in Microorganisms, 10, no. 5 (2022):1069,
https://doi.org/10.3390/microorganisms10051069 . .