TY  - JOUR
AU  - Aleksić, Jelena
AU  - Dokmanović, Marija
AU  - Aleksić, Z.
AU  - Teodorović, Vlado
AU  - Stojić, Velibor
AU  - Trbović, Dejana
AU  - Baltić, Milan Ž.
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/919
AB  - Disadvantage of meat known as boar taint is caused by steroids, androstenone being of particular importance, as well as indole and its derivatives, among which the best known is skatole. The common practice in Europe, in order to control these changes in meat, is castration without anesthesia. This intervention causes pain and stress to animals, thus undermining animal welfare. Many countries considering animal welfare try to find the alternative solutions in order to avoid castration. The alternative to surgical castration and possible solution to the problem of sex odour in the meat, as well as androstenone and skatole contents decrease in the boar's fat is immunological castration of boars (immunocastration). Average skatole content in fat tissue of boars was significantly higher (0.21±0.03 μg/g) compared to skatole content in fat tissue of the castrates, or immunocastrates (0.12±0.02 μg/g). In adipose tissue of the castrates and immunocastrates there was no significant difference in the average content of skatole. The content of androstenone in the adipose tissue of immunocastrates was below detection limits, and the average androstenone level in adipose tissue of boars was 0.66±0.13 μg/g. The obtained results show that immunocastration is justified in consideration of the meat quality and can completely replace castration in male animals, which is in compliance with the preservation of animal welfare in rearing fattening young boars.
AB  - Mani mesa poznatoj kao polni miris mesa doprinose polni steroidi, od kojih je androstenon od posebnog značaja, kao i indol i njegovi derivati, među kojima je najpoznatiji skatol. Najčešća praksa u Evropi u cilju kontrole ove mane mesa je izvođenje kastracije bez anestezije. Izvođenjem ove intervencije prouzrokuje se bol i stres i narušava dobrobit životinja, što je predstavljalo podsticaj da se u mnogim zemljama, gde je poslednjih godina dobrobit životinja od velikog interesa, odustane od kastracije. Napuštanje ove metode zahteva nalaženje alternativnih rešenja u cilju otklanjanja ove mane mesa. Jedna od obećavajućih alternativa hirurškoj kastraciji i potencijalno rešenje problema polnog mirisa mesa, odnosno smanjenja sadržaja androstenona i skatola u masnom tkivu nerastova je imunološ ka kastracija (imunokastracija). Prosečan sadržaj skatola u masnom tkivu nerastova bio je značajno veći (0.21±0.03 μg/g) u odnosu na prosečan sadržaj skatola u masnom tkivu kastrata, odnosno imunokastrata (0.12±0.02 μg/g). U masnom tkivu imunokastrata i kastrata nije utvrđena razlika u prosečnom sadržaju skatola. Sadržaj androstenona u masnom tkivu imunokastrata je bio manji od granice detekcije metode, a u masnom tkivu nerastova prosečan sadržaj androstenona bio je 0.66±0.13 μg/g. Naši rezultati ukazuju da je postupak imunokastracije opravdan sa stanovišta prihvatljivosti mesa i da u potpunosti može da se zameni postupak kastracije muških jedinki, što ide u prilog očuvanju dobrobiti životinja kod uzgoja svinja za tov.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Investigation of the efficacy of immunocastration aimed at the prevention of sex odour in boar meat
T1  - Ispitivanje mogućnosti primene imunokastracije u cilju sprečavanja mane polnog mirisa mesa nerastova
VL  - 62
IS  - 5-6
SP  - 653
EP  - 663
DO  - 10.2298/AVB1206653A
ER  - 

@article{
author = "Aleksić, Jelena and Dokmanović, Marija and Aleksić, Z. and Teodorović, Vlado and Stojić, Velibor and Trbović, Dejana and Baltić, Milan Ž.",
year = "2012",
abstract = "Disadvantage of meat known as boar taint is caused by steroids, androstenone being of particular importance, as well as indole and its derivatives, among which the best known is skatole. The common practice in Europe, in order to control these changes in meat, is castration without anesthesia. This intervention causes pain and stress to animals, thus undermining animal welfare. Many countries considering animal welfare try to find the alternative solutions in order to avoid castration. The alternative to surgical castration and possible solution to the problem of sex odour in the meat, as well as androstenone and skatole contents decrease in the boar's fat is immunological castration of boars (immunocastration). Average skatole content in fat tissue of boars was significantly higher (0.21±0.03 μg/g) compared to skatole content in fat tissue of the castrates, or immunocastrates (0.12±0.02 μg/g). In adipose tissue of the castrates and immunocastrates there was no significant difference in the average content of skatole. The content of androstenone in the adipose tissue of immunocastrates was below detection limits, and the average androstenone level in adipose tissue of boars was 0.66±0.13 μg/g. The obtained results show that immunocastration is justified in consideration of the meat quality and can completely replace castration in male animals, which is in compliance with the preservation of animal welfare in rearing fattening young boars., Mani mesa poznatoj kao polni miris mesa doprinose polni steroidi, od kojih je androstenon od posebnog značaja, kao i indol i njegovi derivati, među kojima je najpoznatiji skatol. Najčešća praksa u Evropi u cilju kontrole ove mane mesa je izvođenje kastracije bez anestezije. Izvođenjem ove intervencije prouzrokuje se bol i stres i narušava dobrobit životinja, što je predstavljalo podsticaj da se u mnogim zemljama, gde je poslednjih godina dobrobit životinja od velikog interesa, odustane od kastracije. Napuštanje ove metode zahteva nalaženje alternativnih rešenja u cilju otklanjanja ove mane mesa. Jedna od obećavajućih alternativa hirurškoj kastraciji i potencijalno rešenje problema polnog mirisa mesa, odnosno smanjenja sadržaja androstenona i skatola u masnom tkivu nerastova je imunološ ka kastracija (imunokastracija). Prosečan sadržaj skatola u masnom tkivu nerastova bio je značajno veći (0.21±0.03 μg/g) u odnosu na prosečan sadržaj skatola u masnom tkivu kastrata, odnosno imunokastrata (0.12±0.02 μg/g). U masnom tkivu imunokastrata i kastrata nije utvrđena razlika u prosečnom sadržaju skatola. Sadržaj androstenona u masnom tkivu imunokastrata je bio manji od granice detekcije metode, a u masnom tkivu nerastova prosečan sadržaj androstenona bio je 0.66±0.13 μg/g. Naši rezultati ukazuju da je postupak imunokastracije opravdan sa stanovišta prihvatljivosti mesa i da u potpunosti može da se zameni postupak kastracije muških jedinki, što ide u prilog očuvanju dobrobiti životinja kod uzgoja svinja za tov.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Investigation of the efficacy of immunocastration aimed at the prevention of sex odour in boar meat, Ispitivanje mogućnosti primene imunokastracije u cilju sprečavanja mane polnog mirisa mesa nerastova",
volume = "62",
number = "5-6",
pages = "653-663",
doi = "10.2298/AVB1206653A"
}

Aleksić, J., Dokmanović, M., Aleksić, Z., Teodorović, V., Stojić, V., Trbović, D.,& Baltić, M. Ž.. (2012). Investigation of the efficacy of immunocastration aimed at the prevention of sex odour in boar meat. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 653-663.
https://doi.org/10.2298/AVB1206653A

Aleksić J, Dokmanović M, Aleksić Z, Teodorović V, Stojić V, Trbović D, Baltić MŽ. Investigation of the efficacy of immunocastration aimed at the prevention of sex odour in boar meat. in Acta Veterinaria-Beograd. 2012;62(5-6):653-663.
doi:10.2298/AVB1206653A .

Aleksić, Jelena, Dokmanović, Marija, Aleksić, Z., Teodorović, Vlado, Stojić, Velibor, Trbović, Dejana, Baltić, Milan Ž., "Investigation of the efficacy of immunocastration aimed at the prevention of sex odour in boar meat" in Acta Veterinaria-Beograd, 62, no. 5-6 (2012):653-663,
https://doi.org/10.2298/AVB1206653A . .

TY  - JOUR
AU  - Kirovski, Danijela
AU  - Sladojević, Željko
AU  - Stojić, Velibor
AU  - Vujanac, Ivan
AU  - Lazarević, Miodrag
AU  - Radovanović, Anita
AU  - Savić, Đ.
AU  - Nedić, Olgica
PY  - 2012
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/860
AB  - The objective of this study was to examine the effect of dietary energy supplementation on hormones that are considered to be the main signals of a shift in energy balance around parturition. Sixty dry cows, 15 days before calving, were chosen and divided into two eaqual groups: control and experimental (GLY). Both groups were fed a standard ration balanced in accordance to the stage of the productive reproductive cycle. Additionally, each cow in the GLY group was given glycerol based dietary energy supplementation (250 mL daily during the dry and 300 mL daily during the lactation period), which provided additional 9.30 MJ NEL during the dry and 13.95 MJ NEL during the early lactation period. Milk production was measured on days 30 and 60 of lactation and milk production was significantly higher in GLY compared to control group at day 60 of lactation (p lt 0.05). Service period and insemination index were used as reproductive outcome parameters. Average service period in the control group was significantly longer than in the GLY group (p lt 0.05). Average insemination index in the control group was not significantly different than the index obtained for the GLY group. Blood samples were taken before the begining of the experiment (15 days before parturition), and at days 7, 30 and 60 of lactation. Concentrations of thyroid hormones, IGF-I, relative abundance of IGFBP-2, IGFBP-3 and IGFBP-4, concentrations of total protein and albumin in the blood were measured. Results showed that at days 7 and 30 after parturition, T4 concentrations were significantly higher (p lt 0.001, respectively) in GLY than in the control group, while T3 concentrations were significantly higher in GLY group only at day 7 after parturition (p lt 0.001). IGF-I concentrations and IGFBP-3 abundance were significantly higher in the GLY compared to the control group in all three examined postpartum periods. IGFBP-2 and IGFBP-4 concentrations were higher in GLY compared to the control group in all three examined postpartum periods, but the difference was significant only on day 60 after parturition (p lt 0.01, respectively). Concentrations of total protein and albumin were significantly higher in GLY compared to the control group in all three examined periods after parturition. Based on these results it can be concluded that peripartum dietary energy supplementation prevent cows' exposure to severe negative energy balance, preserves synthetic activity of hepatocytes and consequently has a positive impact on milk production and reproductive performances in dairy cows.
AB  - Cilj ovog rada je bio da se ispita uticaj energetskog dodatka u ishrani krava na koncentraciju hormona u krvi koji su u periodu oko teljenja glavni pokazatelji promena u energetskom statusu. U tu svrhu, petnaest dana pre teljenja odabrano je 60 krava koje su podeljene u dve jednake grupe: kontrolnu i oglednu (GLY). Obe grupe krava su dobijale identičan obrok usklađen sa proizvodno reproduktivnim ciklusom. Dodatno, kravama GLY grupe je tokom poslednje dve nedelje zasušenja i do 60. dana laktacije dodavan energetski dodatak na bazi glicerola (250 mL dnevno tokom zasušenja odnosno 300 mL nakon teljenja), obezbeđujući dodatnih 9,30 MJ NEL tokom zasušenja, odnosno 13,95 MJ NEL tokom rane laktacije. Kod svih krava je izmerena prosečna dnevna proizvodnja mleka 30. i 60. dana lakatcije, koja je kod GLY grupe bila značajno viša 60. dana (p lt 0,05). Kao pokazatelji reproduktivnog statusa koriščeni su servis period i indeks osemenjavanja. Servis period je bio značajno duži kod kontrolne u odnosu na GLY grupu (p lt 0,05), a vrednost indeksa osemenjavanja se nije značajno razlikovala između grupa. Uzorci krvi krava uzeti su neposredno pre početka ogleda, odnosno 15 dana pre teljenja, kao i 7, 30. i 60. dana laktacije i u njima je određivana koncentracija tireoidnih hormona, IGF-I, relativna zastupljenost IGFBP-2, IGFBP-3 i IGFBP-4, koncentracija ukupnih proteina i albumina. Rezultati su ukazali da je 7. i 30. dana nakon teljenja GLY grupa imala značajno višu koncentraciju T4 (p lt 0,001, pojedinačno) u odnosu na kontrolu, dok je koncentarcija T3 bila značajno viša kod GLY grupe 7. dana nakon teljenja (p lt 0,001). Koncentracija IGF-I i zastupljenost IGFBP-3 je bila značajno viša u krvi krava GLY grupe u odnosu na kontrolu u sva tri ispitivana perioda posle teljenja. Zastupljenost IGFBP-2 i IGFBP-4 je bila viša u krvi krava GLY u odnosu na kontrolnu grupu u sva tri ispitivana perioda postapartalno ali je ova razlika bila značajna jedino 60. dana lakatcije (p lt 0.01, pojedinačno). Koncentracija ukupnih proteina i albumina je, takođe, bila značajno viša u krvi krava GLY grupe u odnosu na kontrolnu grupu u sva tri ispitivana perioda posle teljenja. Na osnovu dobijenih rezultata može se zaključiti da energetski dodatak u ishrani sprečava izloženost krava izrazitom negativnom energetskom bilansu, održava sintetsku funkciju hepatocita i posledično ima pozitivan uticaj na mlečnost i reproduktivne pokazatelje.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Effect of peripartum dietary energy supplementation on thyroid hormones, insulin-like growth factor-i and its binding proteins in early lactation dairy cows
T1  - Uticaj energetskog dodatka u ishrani na koncentraciju tireoidnih hormona, insulinu sličnog faktora rasta-i i njegovih vezujućih proteina u krvi krava tokom rane laktacije
VL  - 62
IS  - 4
SP  - 403
EP  - 419
DO  - 10.2298/AVB1204403K
ER  - 

@article{
author = "Kirovski, Danijela and Sladojević, Željko and Stojić, Velibor and Vujanac, Ivan and Lazarević, Miodrag and Radovanović, Anita and Savić, Đ. and Nedić, Olgica",
year = "2012",
abstract = "The objective of this study was to examine the effect of dietary energy supplementation on hormones that are considered to be the main signals of a shift in energy balance around parturition. Sixty dry cows, 15 days before calving, were chosen and divided into two eaqual groups: control and experimental (GLY). Both groups were fed a standard ration balanced in accordance to the stage of the productive reproductive cycle. Additionally, each cow in the GLY group was given glycerol based dietary energy supplementation (250 mL daily during the dry and 300 mL daily during the lactation period), which provided additional 9.30 MJ NEL during the dry and 13.95 MJ NEL during the early lactation period. Milk production was measured on days 30 and 60 of lactation and milk production was significantly higher in GLY compared to control group at day 60 of lactation (p lt 0.05). Service period and insemination index were used as reproductive outcome parameters. Average service period in the control group was significantly longer than in the GLY group (p lt 0.05). Average insemination index in the control group was not significantly different than the index obtained for the GLY group. Blood samples were taken before the begining of the experiment (15 days before parturition), and at days 7, 30 and 60 of lactation. Concentrations of thyroid hormones, IGF-I, relative abundance of IGFBP-2, IGFBP-3 and IGFBP-4, concentrations of total protein and albumin in the blood were measured. Results showed that at days 7 and 30 after parturition, T4 concentrations were significantly higher (p lt 0.001, respectively) in GLY than in the control group, while T3 concentrations were significantly higher in GLY group only at day 7 after parturition (p lt 0.001). IGF-I concentrations and IGFBP-3 abundance were significantly higher in the GLY compared to the control group in all three examined postpartum periods. IGFBP-2 and IGFBP-4 concentrations were higher in GLY compared to the control group in all three examined postpartum periods, but the difference was significant only on day 60 after parturition (p lt 0.01, respectively). Concentrations of total protein and albumin were significantly higher in GLY compared to the control group in all three examined periods after parturition. Based on these results it can be concluded that peripartum dietary energy supplementation prevent cows' exposure to severe negative energy balance, preserves synthetic activity of hepatocytes and consequently has a positive impact on milk production and reproductive performances in dairy cows., Cilj ovog rada je bio da se ispita uticaj energetskog dodatka u ishrani krava na koncentraciju hormona u krvi koji su u periodu oko teljenja glavni pokazatelji promena u energetskom statusu. U tu svrhu, petnaest dana pre teljenja odabrano je 60 krava koje su podeljene u dve jednake grupe: kontrolnu i oglednu (GLY). Obe grupe krava su dobijale identičan obrok usklađen sa proizvodno reproduktivnim ciklusom. Dodatno, kravama GLY grupe je tokom poslednje dve nedelje zasušenja i do 60. dana laktacije dodavan energetski dodatak na bazi glicerola (250 mL dnevno tokom zasušenja odnosno 300 mL nakon teljenja), obezbeđujući dodatnih 9,30 MJ NEL tokom zasušenja, odnosno 13,95 MJ NEL tokom rane laktacije. Kod svih krava je izmerena prosečna dnevna proizvodnja mleka 30. i 60. dana lakatcije, koja je kod GLY grupe bila značajno viša 60. dana (p lt 0,05). Kao pokazatelji reproduktivnog statusa koriščeni su servis period i indeks osemenjavanja. Servis period je bio značajno duži kod kontrolne u odnosu na GLY grupu (p lt 0,05), a vrednost indeksa osemenjavanja se nije značajno razlikovala između grupa. Uzorci krvi krava uzeti su neposredno pre početka ogleda, odnosno 15 dana pre teljenja, kao i 7, 30. i 60. dana laktacije i u njima je određivana koncentracija tireoidnih hormona, IGF-I, relativna zastupljenost IGFBP-2, IGFBP-3 i IGFBP-4, koncentracija ukupnih proteina i albumina. Rezultati su ukazali da je 7. i 30. dana nakon teljenja GLY grupa imala značajno višu koncentraciju T4 (p lt 0,001, pojedinačno) u odnosu na kontrolu, dok je koncentarcija T3 bila značajno viša kod GLY grupe 7. dana nakon teljenja (p lt 0,001). Koncentracija IGF-I i zastupljenost IGFBP-3 je bila značajno viša u krvi krava GLY grupe u odnosu na kontrolu u sva tri ispitivana perioda posle teljenja. Zastupljenost IGFBP-2 i IGFBP-4 je bila viša u krvi krava GLY u odnosu na kontrolnu grupu u sva tri ispitivana perioda postapartalno ali je ova razlika bila značajna jedino 60. dana lakatcije (p lt 0.01, pojedinačno). Koncentracija ukupnih proteina i albumina je, takođe, bila značajno viša u krvi krava GLY grupe u odnosu na kontrolnu grupu u sva tri ispitivana perioda posle teljenja. Na osnovu dobijenih rezultata može se zaključiti da energetski dodatak u ishrani sprečava izloženost krava izrazitom negativnom energetskom bilansu, održava sintetsku funkciju hepatocita i posledično ima pozitivan uticaj na mlečnost i reproduktivne pokazatelje.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Effect of peripartum dietary energy supplementation on thyroid hormones, insulin-like growth factor-i and its binding proteins in early lactation dairy cows, Uticaj energetskog dodatka u ishrani na koncentraciju tireoidnih hormona, insulinu sličnog faktora rasta-i i njegovih vezujućih proteina u krvi krava tokom rane laktacije",
volume = "62",
number = "4",
pages = "403-419",
doi = "10.2298/AVB1204403K"
}

Kirovski, D., Sladojević, Ž., Stojić, V., Vujanac, I., Lazarević, M., Radovanović, A., Savić, Đ.,& Nedić, O.. (2012). Effect of peripartum dietary energy supplementation on thyroid hormones, insulin-like growth factor-i and its binding proteins in early lactation dairy cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(4), 403-419.
https://doi.org/10.2298/AVB1204403K

Kirovski D, Sladojević Ž, Stojić V, Vujanac I, Lazarević M, Radovanović A, Savić Đ, Nedić O. Effect of peripartum dietary energy supplementation on thyroid hormones, insulin-like growth factor-i and its binding proteins in early lactation dairy cows. in Acta Veterinaria-Beograd. 2012;62(4):403-419.
doi:10.2298/AVB1204403K .

Kirovski, Danijela, Sladojević, Željko, Stojić, Velibor, Vujanac, Ivan, Lazarević, Miodrag, Radovanović, Anita, Savić, Đ., Nedić, Olgica, "Effect of peripartum dietary energy supplementation on thyroid hormones, insulin-like growth factor-i and its binding proteins in early lactation dairy cows" in Acta Veterinaria-Beograd, 62, no. 4 (2012):403-419,
https://doi.org/10.2298/AVB1204403K . .

TY  - JOUR
AU  - Stanimirović, Zoran
AU  - Aleksić, Nevenka
AU  - Stevanović, Jevrosima
AU  - Ćirković, Dragan
AU  - Mirilović, Milorad
AU  - Đelić, Ninoslav
AU  - Stojić, Velibor
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/807
AB  - The aim of this work was the investigation on the efficacy of pulverised sugar dusting on knocking-down Varroa destructor mites and the influence of the dynamics of the treatment on the degree of infestation in honey bee colonies. Two methods were deployed to measure the degree of infestation of bee colonies with V. destructor mites: the sugar shake method and the technique which involves mesh bottom boards equipped with sticky inserts. The research was carried out on 30 strong honey bee colonies in three successive years. The results proved that dusting with pulverised sugar (particle diameter below 40 μm) influenced the fall of V. destructor in comparison with both their fall off prior to the treatment and the negative control. The most discernible effects on the mite fall and the decline in their population in the hives was obtained with dustings repeated at threeday intervals. To conclude, the dynamics of the treatment affected the degree of infestation in bee colonies. However, the efficacy of sugar dusting was significantly lower in comparison with the one of flumethrin (positive control). Thus, in spite of considerable favourable effects on the decline in the degree of infestation with mites, by no means may dusting with pulverised sugar be advised to beekeepers as the one and only means of bee protection against V. destructor. The use of the technique which involves mesh bottom boards equipped with sticky inserts proved more efficacious and sensitive in the judgment of the degree of infestation of bee colonies in comparison with the sugar shake method, which includes dusting 300 bees with 40 g of pulverised sugar (particle diameter below 40 μm).
AB  - Cilj rada bio je da se ispita efikasnost primene šećernog praha u obaranju krpelja Varroa destructor i uticaja dinamike tretmana na stepen infestiranosti pčelinjih zajednica. Za procenu stepena infestacije pčelinjih društava krpeljima V. destructor korišćene su dve metode: metoda rolovanja sa šećerom i metoda žičane podnjače sa lepljivim ulošcima. Istraživanje je obavljeno na 30 jakih pčelinjih zajednica tokom tri godine. Rezultati su ukazali da je zaprašivanje prah šećerom čestica dijametra ispod 40 μm imalo efekat u obaranju krpelja V. destructor, u odnosu na stanje pre tretmana i u odnosu na negativnu kontrolu. Najbolji efekat u obaranju krpelja i smanjenju populacije krpelja u košnici imala je primena prah šećera svakog trećeg dana. To znači da je dinamika tretmana uticala na stepen infestiranosti pčelinjih zajednica. Međutim, efikasnost metode zaprašivanja šećerom u prahu bila je značajno manja u odnosu na efikasnost flumetrina (pozitivnu kontrolu). Stoga se tretman prah šećerom nikako ne može preporučiti pčelarima kao jedina mera zaštite pčelinjih zajednica od ektoparazita V. destructor i pored uočenih pozitivnih efekata na smanjenje stepena infestiranosti tim krpeljom. Korišćenje žičane podnjače sa lepljivim ulošcima predstavlja mnogo efikasniji i osetljiviji metod za procenu stepena infestiranosti pčelinjih zajednica Varroa krpeljom u odnosu na metod rolovanja u tegli sa 300 pčela posutih sa 16 grama šećera u prahu sa česticama čiji je dijametar manji od 40 μm.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The influence of pulverised sugar dusting on the degree of infestation of honey bee colonies with Varroa destructor
T1  - Uticaj tretmana šećerom u prahu na stepen infestiranosti pčelinjih zajednica ektoparazitom Varroa destructor
VL  - 61
IS  - 2-3
SP  - 309
EP  - 325
DO  - 10.2298/AVB1103309S
ER  - 

@article{
author = "Stanimirović, Zoran and Aleksić, Nevenka and Stevanović, Jevrosima and Ćirković, Dragan and Mirilović, Milorad and Đelić, Ninoslav and Stojić, Velibor",
year = "2011",
abstract = "The aim of this work was the investigation on the efficacy of pulverised sugar dusting on knocking-down Varroa destructor mites and the influence of the dynamics of the treatment on the degree of infestation in honey bee colonies. Two methods were deployed to measure the degree of infestation of bee colonies with V. destructor mites: the sugar shake method and the technique which involves mesh bottom boards equipped with sticky inserts. The research was carried out on 30 strong honey bee colonies in three successive years. The results proved that dusting with pulverised sugar (particle diameter below 40 μm) influenced the fall of V. destructor in comparison with both their fall off prior to the treatment and the negative control. The most discernible effects on the mite fall and the decline in their population in the hives was obtained with dustings repeated at threeday intervals. To conclude, the dynamics of the treatment affected the degree of infestation in bee colonies. However, the efficacy of sugar dusting was significantly lower in comparison with the one of flumethrin (positive control). Thus, in spite of considerable favourable effects on the decline in the degree of infestation with mites, by no means may dusting with pulverised sugar be advised to beekeepers as the one and only means of bee protection against V. destructor. The use of the technique which involves mesh bottom boards equipped with sticky inserts proved more efficacious and sensitive in the judgment of the degree of infestation of bee colonies in comparison with the sugar shake method, which includes dusting 300 bees with 40 g of pulverised sugar (particle diameter below 40 μm)., Cilj rada bio je da se ispita efikasnost primene šećernog praha u obaranju krpelja Varroa destructor i uticaja dinamike tretmana na stepen infestiranosti pčelinjih zajednica. Za procenu stepena infestacije pčelinjih društava krpeljima V. destructor korišćene su dve metode: metoda rolovanja sa šećerom i metoda žičane podnjače sa lepljivim ulošcima. Istraživanje je obavljeno na 30 jakih pčelinjih zajednica tokom tri godine. Rezultati su ukazali da je zaprašivanje prah šećerom čestica dijametra ispod 40 μm imalo efekat u obaranju krpelja V. destructor, u odnosu na stanje pre tretmana i u odnosu na negativnu kontrolu. Najbolji efekat u obaranju krpelja i smanjenju populacije krpelja u košnici imala je primena prah šećera svakog trećeg dana. To znači da je dinamika tretmana uticala na stepen infestiranosti pčelinjih zajednica. Međutim, efikasnost metode zaprašivanja šećerom u prahu bila je značajno manja u odnosu na efikasnost flumetrina (pozitivnu kontrolu). Stoga se tretman prah šećerom nikako ne može preporučiti pčelarima kao jedina mera zaštite pčelinjih zajednica od ektoparazita V. destructor i pored uočenih pozitivnih efekata na smanjenje stepena infestiranosti tim krpeljom. Korišćenje žičane podnjače sa lepljivim ulošcima predstavlja mnogo efikasniji i osetljiviji metod za procenu stepena infestiranosti pčelinjih zajednica Varroa krpeljom u odnosu na metod rolovanja u tegli sa 300 pčela posutih sa 16 grama šećera u prahu sa česticama čiji je dijametar manji od 40 μm.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The influence of pulverised sugar dusting on the degree of infestation of honey bee colonies with Varroa destructor, Uticaj tretmana šećerom u prahu na stepen infestiranosti pčelinjih zajednica ektoparazitom Varroa destructor",
volume = "61",
number = "2-3",
pages = "309-325",
doi = "10.2298/AVB1103309S"
}

Stanimirović, Z., Aleksić, N., Stevanović, J., Ćirković, D., Mirilović, M., Đelić, N.,& Stojić, V.. (2011). The influence of pulverised sugar dusting on the degree of infestation of honey bee colonies with Varroa destructor. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(2-3), 309-325.
https://doi.org/10.2298/AVB1103309S

Stanimirović Z, Aleksić N, Stevanović J, Ćirković D, Mirilović M, Đelić N, Stojić V. The influence of pulverised sugar dusting on the degree of infestation of honey bee colonies with Varroa destructor. in Acta Veterinaria-Beograd. 2011;61(2-3):309-325.
doi:10.2298/AVB1103309S .

Stanimirović, Zoran, Aleksić, Nevenka, Stevanović, Jevrosima, Ćirković, Dragan, Mirilović, Milorad, Đelić, Ninoslav, Stojić, Velibor, "The influence of pulverised sugar dusting on the degree of infestation of honey bee colonies with Varroa destructor" in Acta Veterinaria-Beograd, 61, no. 2-3 (2011):309-325,
https://doi.org/10.2298/AVB1103309S . .

TY  - JOUR
AU  - Kirovski, Danijela
AU  - Lazarević, Miodrag
AU  - Stojić, Velibor
AU  - Šamanc, Horea
AU  - Vujanac, Ivan
AU  - Nedić, Olgica
AU  - Masnikosa, Romana
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/808
AB  - The aim of this study was to examine changes in some hormones concentrations in calves during the first 32 hours of neonatal life and to estimate their association with glycemia. Thyrty two Holstein breed calves were selected for the study. Blood samples were taken at 30, 60 and 90 minutes postnatal. Calves received pooled colostrum: primary colostum (1.5 L, 2 hours after birth), secondary colostrum (2 L, 14 hours after birth) and tertiary colostrum (2 L, 26 hours after birth). Blood samples were taken at hours 5, 20 and 32 of neonatal life. Concentrations of glucose, insulin, cortisol, thyroid hormones and IGF-I and abundance of IGFBP-1, IGFBP-2 and IGFBP-3 were determined in the blood serum. The T3/T4 ratio was also calculated. Calves were born hypoglycemic (glycemia was 2.56±1.05 mmol/L at birth). Thereafter, glycemia significantly increased (p lt 0.001) to 3.05±0.89 mmol/L at min 90. Glucose concentration showed a further increase after colostrum intake and was significantly higher than at the initial value in all examined periods (p lt 0.001). During the first 90 minutes of neonatal life insulinemia decreased significantly (p lt 0.001) compared to initial value (26.33±10.05 μIU/L) and it measured 18.66±5.56 μIU/L at min 90. Cortisolemia was highest at minute 30 (85.08±19.36 nmol/L) and than decreased until the end of the experiment (p lt 0.001) compared to initial values in samples obtained during the period of colostrum intake. A significantly high correlation was determined between glycemia and cortisolemia in all examined periods before the first colostrums intake (r2=0.854; p lt 0.01 at min 30; r2=0.742; p lt 0.01 at min 60 and r2=0.551; p lt 0.01 at min 90). T4 concentrations significantly increased during the first 2 hours, while T3 concentrations decreased, significantly from min 30 to min 90 postnatal (p lt 0.05). T3/T4 ratio significantly increased during the first 2 hours of neonatal life. After first colostrum intake, concentrations of both hormones rose significantly compared to the initial level, but T3/T4 ratio did not change and maintained the value determined at minute 90. IGF- 1 concentrations significantly decreased during the first 2 postnatal hours. A significant positive correlation was observed between IGF-1 concentration and insulinemia (r2=0.463; p lt 0.05 at min 30, r2=0.662; p lt 0.01 at min 60 and r2=0.583; p lt 0.01 at min 90). IGFBP-3 abundance significantly decreased, while IGFBP-1 significantly increased in this period. IGFBP-2 abundance was highest at birth. Results presented in this study indicate that the increase in glucose concentration during the first 2 hours of neonatal life, before the first colostrum intake is mainly the result of increased activity of the adrenal cortex in cortisol secretion and extrathyroidal tissue thus providing sufficient triiodothyronine. Immaturity of mechanisms responsible for insulin secretion provides the dominance of catabolic processes. Changes of the IGF system provide a rise of glucose concentration and establishment of energy balance.
AB  - Cilj ovog rada je bio da se ispitaju promene koncentracije pojedinih hormona kod novorođene teladi u prvim satima neonatalnog života i utvrdi njihov uticaj na glikemiju. Odabrana su 32 novorođena teleta Holštajn rase kojima je 30, 60. i 90. minuta postnatalnog života uzeta krv. Telad su bila napajana pulovima kolostruma. Pul primarnog kolostruma davan je u količinama od po 1,5 litar 2 sata nakon rođenja, dok su pulovi sekundarnog i tercijarnog kolostruma davani 12, odnosno 24 sata kasnije, u količinama od po 2 litra. Tokom perioda kolostralnog napoja, teladi je uzorkovana krv 5, 20 i 32. sata nakon rođenja. U uzorcima krvi određ ivana je koncentracija glukoze, insulina, kortizola, tireoidnih hormona i IGF-I, kao i zastupljenost IGFBP-1, IGFBP-2 i IGFBP-3. Takođe je obračunat indeks konverzije T3 u T4. Telad su bila rođena u stanju hipoglikemije (koncentracija glukoze na rođenju je iznosila 2,56 ± 1,05 mmol/l). Nakon toga, glikemija je značajno porasla (p lt 0,001) do 3,05 ±0,89 mmol/l (90. minut). Porast koncentracije glukoze je nastavljen i nakon unosa kolostruma, tako da je glikemija u svim ispitivanim uzorcima bila značajno veća u odnosu na početnu vrednost (p lt 0,001). Tokom prvih 90 minuta neonatalnog života, koncentracija insulina se značajno smanjivala (p lt  0,001) u odnosu na početnu vrednost (26,33 ± 10,05 μIU/l) tako da je 90. minuta postnatalnog života bila 18,66 ± 5,56 μIU/l. Porast insulinemije nakon unosa kolostruma nije bio značajan u odnosu na vrednost određenu 90. minuta. Koncentracija kortizola je bila najviša 30 minuta nakon teljenja (85,08 ± 19,36 nmol/l) a zatim je opadala do kraja perioda ispitivanja i to značajno u odnosu na početnu vrednost (p lt 0,001) u uzorcima dobijenim nakon unosa kolostruma. Visoka pozitivna korelacija je utvrđena između glikemije i kortizolemije u svim ispitivanim terminima pre kolostralnog napoja (r2 = 0,854 u 30. minutu; r2 = 0,742 u 60. minutu i r2= 0,551 u 90. minutu). Koncentracija T4 je značajno rasla tokom prva dva sata neonatalnog života, dok se koncentracija T3 smanjila, značajno od 30. do 90. minuta neonatalnog života (p lt 0,05). Konverzija T3 u T4 je značajno porasla tokom prva dva sata života. Nakon unosa kolostruma, koncentracija oba tireoidna hormona se povećavala (značajno u odnosu na početnu vrednost) a indeks konverzije se nije menjao, već se zadržao na vrednosti ustanovljenoj 90. minuta života. Koncentracija IGF-1 se značajno smanjivala tokom prva 2 sata neonatalnog života. Koncentracija IGF-1 je bila u visokoj pozitivnoj korelaciji sa insulinemijom (r2= 0,463 za 30. minut, r2=0,662 za 60. minut i r2=0,583 za 90. minut). Zastupljenost IGFBP-3 se značajno smanjivala, dok se zastupljenost IGFBP-1 značajno povećavala u ovom periodu. Zastupljenost IGFBP-2 je bila najveća na rođenju. Rezultati prikazani u ovom radu ukazuju da je porast glikemije u prvim satima života, pre unosa kolostruma, prevashodno posledica pojačane aktivnosti kore nadbubrega u sekreciji kortizola i dejodinaza u ekstratireoidnim tkivima koje obezbeđuju povećanu sintezu T3. Sistemi odgovorni za sintezu insulina nisu potpuno funkcionalni u ovom periodu, omogućavajući prevagu kataboličkih u odnosu na anaboličke procese. Promene unutar IGF sistema omogućavaju porast glikemije i uspostavljanje energetske ravnoteže.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Hormonal status and regulation of glycemia in neonatal calves during the first hours of postnatal life
T1  - Hormonalni status i regulacija glikemije kod novorođene teladi tokom prvih sati postnatalnog života
VL  - 61
IS  - 4
SP  - 349
EP  - 361
DO  - 10.2298/AVB1104349K
ER  - 

@article{
author = "Kirovski, Danijela and Lazarević, Miodrag and Stojić, Velibor and Šamanc, Horea and Vujanac, Ivan and Nedić, Olgica and Masnikosa, Romana",
year = "2011",
abstract = "The aim of this study was to examine changes in some hormones concentrations in calves during the first 32 hours of neonatal life and to estimate their association with glycemia. Thyrty two Holstein breed calves were selected for the study. Blood samples were taken at 30, 60 and 90 minutes postnatal. Calves received pooled colostrum: primary colostum (1.5 L, 2 hours after birth), secondary colostrum (2 L, 14 hours after birth) and tertiary colostrum (2 L, 26 hours after birth). Blood samples were taken at hours 5, 20 and 32 of neonatal life. Concentrations of glucose, insulin, cortisol, thyroid hormones and IGF-I and abundance of IGFBP-1, IGFBP-2 and IGFBP-3 were determined in the blood serum. The T3/T4 ratio was also calculated. Calves were born hypoglycemic (glycemia was 2.56±1.05 mmol/L at birth). Thereafter, glycemia significantly increased (p lt 0.001) to 3.05±0.89 mmol/L at min 90. Glucose concentration showed a further increase after colostrum intake and was significantly higher than at the initial value in all examined periods (p lt 0.001). During the first 90 minutes of neonatal life insulinemia decreased significantly (p lt 0.001) compared to initial value (26.33±10.05 μIU/L) and it measured 18.66±5.56 μIU/L at min 90. Cortisolemia was highest at minute 30 (85.08±19.36 nmol/L) and than decreased until the end of the experiment (p lt 0.001) compared to initial values in samples obtained during the period of colostrum intake. A significantly high correlation was determined between glycemia and cortisolemia in all examined periods before the first colostrums intake (r2=0.854; p lt 0.01 at min 30; r2=0.742; p lt 0.01 at min 60 and r2=0.551; p lt 0.01 at min 90). T4 concentrations significantly increased during the first 2 hours, while T3 concentrations decreased, significantly from min 30 to min 90 postnatal (p lt 0.05). T3/T4 ratio significantly increased during the first 2 hours of neonatal life. After first colostrum intake, concentrations of both hormones rose significantly compared to the initial level, but T3/T4 ratio did not change and maintained the value determined at minute 90. IGF- 1 concentrations significantly decreased during the first 2 postnatal hours. A significant positive correlation was observed between IGF-1 concentration and insulinemia (r2=0.463; p lt 0.05 at min 30, r2=0.662; p lt 0.01 at min 60 and r2=0.583; p lt 0.01 at min 90). IGFBP-3 abundance significantly decreased, while IGFBP-1 significantly increased in this period. IGFBP-2 abundance was highest at birth. Results presented in this study indicate that the increase in glucose concentration during the first 2 hours of neonatal life, before the first colostrum intake is mainly the result of increased activity of the adrenal cortex in cortisol secretion and extrathyroidal tissue thus providing sufficient triiodothyronine. Immaturity of mechanisms responsible for insulin secretion provides the dominance of catabolic processes. Changes of the IGF system provide a rise of glucose concentration and establishment of energy balance., Cilj ovog rada je bio da se ispitaju promene koncentracije pojedinih hormona kod novorođene teladi u prvim satima neonatalnog života i utvrdi njihov uticaj na glikemiju. Odabrana su 32 novorođena teleta Holštajn rase kojima je 30, 60. i 90. minuta postnatalnog života uzeta krv. Telad su bila napajana pulovima kolostruma. Pul primarnog kolostruma davan je u količinama od po 1,5 litar 2 sata nakon rođenja, dok su pulovi sekundarnog i tercijarnog kolostruma davani 12, odnosno 24 sata kasnije, u količinama od po 2 litra. Tokom perioda kolostralnog napoja, teladi je uzorkovana krv 5, 20 i 32. sata nakon rođenja. U uzorcima krvi određ ivana je koncentracija glukoze, insulina, kortizola, tireoidnih hormona i IGF-I, kao i zastupljenost IGFBP-1, IGFBP-2 i IGFBP-3. Takođe je obračunat indeks konverzije T3 u T4. Telad su bila rođena u stanju hipoglikemije (koncentracija glukoze na rođenju je iznosila 2,56 ± 1,05 mmol/l). Nakon toga, glikemija je značajno porasla (p lt 0,001) do 3,05 ±0,89 mmol/l (90. minut). Porast koncentracije glukoze je nastavljen i nakon unosa kolostruma, tako da je glikemija u svim ispitivanim uzorcima bila značajno veća u odnosu na početnu vrednost (p lt 0,001). Tokom prvih 90 minuta neonatalnog života, koncentracija insulina se značajno smanjivala (p lt  0,001) u odnosu na početnu vrednost (26,33 ± 10,05 μIU/l) tako da je 90. minuta postnatalnog života bila 18,66 ± 5,56 μIU/l. Porast insulinemije nakon unosa kolostruma nije bio značajan u odnosu na vrednost određenu 90. minuta. Koncentracija kortizola je bila najviša 30 minuta nakon teljenja (85,08 ± 19,36 nmol/l) a zatim je opadala do kraja perioda ispitivanja i to značajno u odnosu na početnu vrednost (p lt 0,001) u uzorcima dobijenim nakon unosa kolostruma. Visoka pozitivna korelacija je utvrđena između glikemije i kortizolemije u svim ispitivanim terminima pre kolostralnog napoja (r2 = 0,854 u 30. minutu; r2 = 0,742 u 60. minutu i r2= 0,551 u 90. minutu). Koncentracija T4 je značajno rasla tokom prva dva sata neonatalnog života, dok se koncentracija T3 smanjila, značajno od 30. do 90. minuta neonatalnog života (p lt 0,05). Konverzija T3 u T4 je značajno porasla tokom prva dva sata života. Nakon unosa kolostruma, koncentracija oba tireoidna hormona se povećavala (značajno u odnosu na početnu vrednost) a indeks konverzije se nije menjao, već se zadržao na vrednosti ustanovljenoj 90. minuta života. Koncentracija IGF-1 se značajno smanjivala tokom prva 2 sata neonatalnog života. Koncentracija IGF-1 je bila u visokoj pozitivnoj korelaciji sa insulinemijom (r2= 0,463 za 30. minut, r2=0,662 za 60. minut i r2=0,583 za 90. minut). Zastupljenost IGFBP-3 se značajno smanjivala, dok se zastupljenost IGFBP-1 značajno povećavala u ovom periodu. Zastupljenost IGFBP-2 je bila najveća na rođenju. Rezultati prikazani u ovom radu ukazuju da je porast glikemije u prvim satima života, pre unosa kolostruma, prevashodno posledica pojačane aktivnosti kore nadbubrega u sekreciji kortizola i dejodinaza u ekstratireoidnim tkivima koje obezbeđuju povećanu sintezu T3. Sistemi odgovorni za sintezu insulina nisu potpuno funkcionalni u ovom periodu, omogućavajući prevagu kataboličkih u odnosu na anaboličke procese. Promene unutar IGF sistema omogućavaju porast glikemije i uspostavljanje energetske ravnoteže.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Hormonal status and regulation of glycemia in neonatal calves during the first hours of postnatal life, Hormonalni status i regulacija glikemije kod novorođene teladi tokom prvih sati postnatalnog života",
volume = "61",
number = "4",
pages = "349-361",
doi = "10.2298/AVB1104349K"
}

Kirovski, D., Lazarević, M., Stojić, V., Šamanc, H., Vujanac, I., Nedić, O.,& Masnikosa, R.. (2011). Hormonal status and regulation of glycemia in neonatal calves during the first hours of postnatal life. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(4), 349-361.
https://doi.org/10.2298/AVB1104349K

Kirovski D, Lazarević M, Stojić V, Šamanc H, Vujanac I, Nedić O, Masnikosa R. Hormonal status and regulation of glycemia in neonatal calves during the first hours of postnatal life. in Acta Veterinaria-Beograd. 2011;61(4):349-361.
doi:10.2298/AVB1104349K .

Kirovski, Danijela, Lazarević, Miodrag, Stojić, Velibor, Šamanc, Horea, Vujanac, Ivan, Nedić, Olgica, Masnikosa, Romana, "Hormonal status and regulation of glycemia in neonatal calves during the first hours of postnatal life" in Acta Veterinaria-Beograd, 61, no. 4 (2011):349-361,
https://doi.org/10.2298/AVB1104349K . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Kirovski, Danijela
AU  - Stojić, Velibor
AU  - Stojanović, Dragica
AU  - Vujanac, Ivan
AU  - Prodanović, Radiša
AU  - Bojković-Kovačević, Slavica
PY  - 2011
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/828
AB  - The aim of this study was to examine the possibility of using results of metabolic profile test in the prediction and diagnosis of fatty liver in Holstein cows. Forty dry cows, 5 to 7 days before calving, were chosen from the commercial dairy herd and included in the study. Four blood samples were taken by jugular venipuncture from each animal: 5 to 7 days before expected calving (dry period), as well as on day 12, 30 and 60 of lactation. Concentrations of glucose, β-hydroxybutyrate (BHBA), total protein, albumin, urea, total bilirubin, calcium and phosphorus concentrations were measured in all blood samples. On day 12 after calving, liver percutaneous biopsies were obtained using a biopsy instrument. Liver tissue lipid content was determined by pathohistological determination. Cows were divided into two groups of equal size based on the degree of lipid accumulation in the liver: healthy cows (0.00 fat, n = 20) and cows with fatty liver syndrome (>20% fat, n = 20). Milking was measured each day from day 7 to day 60 of lactation. Results showed that 5 to 7 days before calving, as well as 12 days after calving, BHBA concentrations were significantly higher (p lt 0.001, respectively) in diseased cows than in healthy cows. Additionally, the concentration of glucose was significantly lower (p lt 0.01) and the concentration of total bilirubin significantly higher (p lt 0.001) in diseased compared to healthy cows, at day 12 of lactation. Thirty days after calving, concentrations of albumin, glucose, BHBA and Ca were significantly lower in diseased compared to healthy cows (p lt 0.05, p lt 0.01, p lt 0.01 and p lt 0.001, respectively). At day 60 after calving, concentrations of biochemical parameters did not differ between diseased and healthy cows. The lactation curve differed in diseased compared to healthy cows, starting from day 26 of lactation. Starting form that day until day 60 of lactation average daily milk production was significantly higher in healthy than in diseased cows, except on day 32 of lactation when the difference was not significant. Based on these results it can be concluded that the metabolic profile test may be a reliable tool for the prediction and diagnosis of fatty liver in Holstein cows.
AB  - Cilj ovog rada je bio da se ispita mogućnost korišćenja rezultata dobijenih određivanjem metaboličkog profila u predviđanju i dijagnostikovanju sindroma masne jetre kod krava holštajn rase. U ogled je bilo uključeno 40 krava holštajn rase u periodu zasušenja, odnosno 5 do 7 dana pre očekivanog termina teljenja. Kravama su uzorci krvi uzeti punkcijom vene jugularis 4 puta i to 5 do 7 dana pre očekivanog termina teljenja, kao i 12., 30. i 60. dana laktacije. U uzorcima krvi određivane su koncentracije ukupnih proteina, albumina, uree, glukoze, BHBA, ukupnog bilirubina, kalcijuma i fosfora. Dvanaest dana nakon teljenja perkutanom biopsijom uzet je uzorak jetre u kome je, patohistološkim pregledom, određen sadržaj ukupnih lipida. Nakon pregleda tkiva jetre krave su podeljene u dve jednake grupe. Krave kod kojih je stepen zamašćenje jetre bio veći od 20% (obolele krave, n = 20) i krave bez prisustva masnih kapljica u uzorcima jetre (zdrave krave, n = 20). Mlečnost je praćena svakodnevno od sedmog do 60. dana laktacije. Dobijeni rezultati su ukazali da je 5 do 7 dana pre očekivanog termina teljenja kao i 12. dana laktacije koncentracija BHBA bila značajno viša (p lt 0.001, pojedinačno) kod obolelih nego kod zdravih krava. Takođe, 12 dana nakon teljenja, koncentracija glukoze (p lt 0.01) je bila značajno niža, a koncentracija bilirubina značajno viša (p lt 0.001) kod obolelih nego zdravih krava. Tridesetog dana nakon teljenja koncentracija albumina, glukoze, BHBA i Ca je bila značajno niža kod obolelih u odnosu na zdrave krave (p lt 0,05; p lt 0,01; p lt 0,01 i p lt 0,001, pojedinač no). Šezdesetog dana nakon teljenja koncentracije ispitivanih parametara se nisu značajno razlikovale između obolelih i zdravih krava. Laktaciona kriva je imala različiti trend kod zdravih i bolesnih krava počevši od 26. dana nakon teljenja. Od tog dana pa do 60. dana laktacije prosečna dnevna mlečnost je bila značajno viša kod zdravih nego obolelih krava, izuzev 32. dana laktacije kada ta razlika nije bila značajna. Na osnovu dobijenih rezultata može se zaključiti da se metabolički profil može sa visokom pouzdanošću koristiti u predviđanju i dijagnostici zamašćenja jetre kod krava Holštajn rase.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Application of the metabolic profile test in the prediction and diagnosis of fatty liver in Holstein cows
T1  - Primena metaboličkog profila u predviđanju i dijagnozi masne jetre kod krava holštajn rase
VL  - 61
IS  - 5-6
SP  - 543
EP  - 553
DO  - 10.2298/AVB1106543S
ER  - 

@article{
author = "Šamanc, Horea and Kirovski, Danijela and Stojić, Velibor and Stojanović, Dragica and Vujanac, Ivan and Prodanović, Radiša and Bojković-Kovačević, Slavica",
year = "2011",
abstract = "The aim of this study was to examine the possibility of using results of metabolic profile test in the prediction and diagnosis of fatty liver in Holstein cows. Forty dry cows, 5 to 7 days before calving, were chosen from the commercial dairy herd and included in the study. Four blood samples were taken by jugular venipuncture from each animal: 5 to 7 days before expected calving (dry period), as well as on day 12, 30 and 60 of lactation. Concentrations of glucose, β-hydroxybutyrate (BHBA), total protein, albumin, urea, total bilirubin, calcium and phosphorus concentrations were measured in all blood samples. On day 12 after calving, liver percutaneous biopsies were obtained using a biopsy instrument. Liver tissue lipid content was determined by pathohistological determination. Cows were divided into two groups of equal size based on the degree of lipid accumulation in the liver: healthy cows (0.00 fat, n = 20) and cows with fatty liver syndrome (>20% fat, n = 20). Milking was measured each day from day 7 to day 60 of lactation. Results showed that 5 to 7 days before calving, as well as 12 days after calving, BHBA concentrations were significantly higher (p lt 0.001, respectively) in diseased cows than in healthy cows. Additionally, the concentration of glucose was significantly lower (p lt 0.01) and the concentration of total bilirubin significantly higher (p lt 0.001) in diseased compared to healthy cows, at day 12 of lactation. Thirty days after calving, concentrations of albumin, glucose, BHBA and Ca were significantly lower in diseased compared to healthy cows (p lt 0.05, p lt 0.01, p lt 0.01 and p lt 0.001, respectively). At day 60 after calving, concentrations of biochemical parameters did not differ between diseased and healthy cows. The lactation curve differed in diseased compared to healthy cows, starting from day 26 of lactation. Starting form that day until day 60 of lactation average daily milk production was significantly higher in healthy than in diseased cows, except on day 32 of lactation when the difference was not significant. Based on these results it can be concluded that the metabolic profile test may be a reliable tool for the prediction and diagnosis of fatty liver in Holstein cows., Cilj ovog rada je bio da se ispita mogućnost korišćenja rezultata dobijenih određivanjem metaboličkog profila u predviđanju i dijagnostikovanju sindroma masne jetre kod krava holštajn rase. U ogled je bilo uključeno 40 krava holštajn rase u periodu zasušenja, odnosno 5 do 7 dana pre očekivanog termina teljenja. Kravama su uzorci krvi uzeti punkcijom vene jugularis 4 puta i to 5 do 7 dana pre očekivanog termina teljenja, kao i 12., 30. i 60. dana laktacije. U uzorcima krvi određivane su koncentracije ukupnih proteina, albumina, uree, glukoze, BHBA, ukupnog bilirubina, kalcijuma i fosfora. Dvanaest dana nakon teljenja perkutanom biopsijom uzet je uzorak jetre u kome je, patohistološkim pregledom, određen sadržaj ukupnih lipida. Nakon pregleda tkiva jetre krave su podeljene u dve jednake grupe. Krave kod kojih je stepen zamašćenje jetre bio veći od 20% (obolele krave, n = 20) i krave bez prisustva masnih kapljica u uzorcima jetre (zdrave krave, n = 20). Mlečnost je praćena svakodnevno od sedmog do 60. dana laktacije. Dobijeni rezultati su ukazali da je 5 do 7 dana pre očekivanog termina teljenja kao i 12. dana laktacije koncentracija BHBA bila značajno viša (p lt 0.001, pojedinačno) kod obolelih nego kod zdravih krava. Takođe, 12 dana nakon teljenja, koncentracija glukoze (p lt 0.01) je bila značajno niža, a koncentracija bilirubina značajno viša (p lt 0.001) kod obolelih nego zdravih krava. Tridesetog dana nakon teljenja koncentracija albumina, glukoze, BHBA i Ca je bila značajno niža kod obolelih u odnosu na zdrave krave (p lt 0,05; p lt 0,01; p lt 0,01 i p lt 0,001, pojedinač no). Šezdesetog dana nakon teljenja koncentracije ispitivanih parametara se nisu značajno razlikovale između obolelih i zdravih krava. Laktaciona kriva je imala različiti trend kod zdravih i bolesnih krava počevši od 26. dana nakon teljenja. Od tog dana pa do 60. dana laktacije prosečna dnevna mlečnost je bila značajno viša kod zdravih nego obolelih krava, izuzev 32. dana laktacije kada ta razlika nije bila značajna. Na osnovu dobijenih rezultata može se zaključiti da se metabolički profil može sa visokom pouzdanošću koristiti u predviđanju i dijagnostici zamašćenja jetre kod krava Holštajn rase.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Application of the metabolic profile test in the prediction and diagnosis of fatty liver in Holstein cows, Primena metaboličkog profila u predviđanju i dijagnozi masne jetre kod krava holštajn rase",
volume = "61",
number = "5-6",
pages = "543-553",
doi = "10.2298/AVB1106543S"
}

Šamanc, H., Kirovski, D., Stojić, V., Stojanović, D., Vujanac, I., Prodanović, R.,& Bojković-Kovačević, S.. (2011). Application of the metabolic profile test in the prediction and diagnosis of fatty liver in Holstein cows. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 61(5-6), 543-553.
https://doi.org/10.2298/AVB1106543S

Šamanc H, Kirovski D, Stojić V, Stojanović D, Vujanac I, Prodanović R, Bojković-Kovačević S. Application of the metabolic profile test in the prediction and diagnosis of fatty liver in Holstein cows. in Acta Veterinaria-Beograd. 2011;61(5-6):543-553.
doi:10.2298/AVB1106543S .

Šamanc, Horea, Kirovski, Danijela, Stojić, Velibor, Stojanović, Dragica, Vujanac, Ivan, Prodanović, Radiša, Bojković-Kovačević, Slavica, "Application of the metabolic profile test in the prediction and diagnosis of fatty liver in Holstein cows" in Acta Veterinaria-Beograd, 61, no. 5-6 (2011):543-553,
https://doi.org/10.2298/AVB1106543S . .

TY  - JOUR
AU  - Gvozdić, Dragan
AU  - Aleksić, Jelena
AU  - Fratrić, Natalija
AU  - Jakić-Dimić, Dobrila
AU  - Stojić, Velibor
AU  - Pavlović, V.
AU  - Pavlović, Miloš
AU  - Vakanjac, Slobodanka
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/675
AB  - Oral zeolite treatement effects on the blood serum free amino acid pattern in newborn calves was investigated. The total number of 30 newborn Holstein calves of both sexes, weighting 35±3 kg (mean±SD), were immediately after parturition separated from their dams and placed in individual pens. Calves were divided in two experimental groups, 15 calves each. All calves were bottle-fed twice/ day (1.5 L/meal) during the first 48 hours after delivery, in 12 hour intervals, with their mother's first (during 24 hours postpartum) or second colostrum (at 24-48 hours postpartum), starting two hours after delivery. Zeolite suspension (20 mL, 25% suspension in distilled water) was added to every meal for treated calves. Colostrum samples were collected from six cows at 0-12h and 24h after delivery. Total and colostral whey protein concentrations were determined using the colorimetric method. Blood samples were taken from the jugular vein of calves at 6, 16, 30 and 40 hours after birth. After spontaneous coagulation at room temperature blood serum was separated and stored at -20°C until analyzed. Total protein concentration was determined by the colorimetric method. Blood serum immunoglobulin G (IgG) concentrations were determined using single radial immunodiffusion (sRID) plates. Pooled blood serum free amino acids (aspartic acid - Asp, glutamic acid - Glu, serine - Ser, histidine - His, glycine - Gly, threonine - Thr, alanine - Ala, proline - Pro, tyrosine - Tyr, arginine - Arg, valine - Val, methionine - Met, Leucine - Leu, Isoleucine - Ile, phenylalanine - Phe) were identified and quantified using high-performance liquid chromatography (HPLC, GBC Australia). Total and colostral whey protein concentrations were significantly higher in the first colostrum and decreased between 50-75% at 24-48 hours later on. Mean blood serum IgG concentration was significantly increased at 6 and 16 hours in the treated calves (26±7:20±5 and 55±15:42±13 g/L, p lt 0.05). Blood serum free amino acids (AA) first were separated at nonessential and essential AA (NEAA and EAA, respectively), both being increased at all time intervals after birth in treated calves. However, when the pooled blood serum free AA were clustered according to polarity and electrical charge and presented as relative values (% of the control group values) at the 6h there was a massive increase of polar positive (Arg, His), polar neutral (Ser, Thr, Tyr) and nonpolar neutral free AA (except Met). The minimal effect of oral zeolite treatment was on the negative polar blood serum free AA concentration (Asp and Glu).
AB  - U ovom radu su izneti rezultati ispitivanja uticaja oralnog tretmana zeolitom na koncentraciju slobodnih aminokiselina u krvnom serumu kod novorođene teladi. Ispitivanje je izvršeno na ukupno 30 novorođenih teladi, oba pola, prosečne telesne mase 35±3 kg (srednja vrednost±SD), koja su neposredno nakon partusa odvojena od majke i smeštena u individualne bokseve. Telad su podeljena u dve ogledne grupe sa po 15 životinja u svakoj grupi. Sva telad su hranjena dva puta dnevno (1.5 L/obroku) u toku prvih 48 sati nakon partusa kolostrumom njihovih majki, u intervalu od 12 sati, počevši od 2 sata nakon partusa. U toku prva 24 sata telad su hranjena prvim kolostrumom (skupljen u toku prvih 24 sata) dok su u sledeća 24 sata hranjena drugim kolostrumom (skupljen u periodu od 24-48 sati nakon partusa). Tretirana grupa teladi dobijala je sa svakim obrokom suspenziju zeolita (20 mL, 25% suspenzije zeolita u destilovanoj vodi). Uzorci kolostruma uzimani su od 6 krava u periodu od 0 - 12 sati i 24 sata nakon partusa i kolorimetrijskom metodom je određivana koncentracija proteina u kolostrumu i kolostralnom mlečnom serumu. Uzorci krvi od teladi uzimani su iz v. jugularis 6, 16, 30. i 40. sata nakon partusa, nakon spontane koagulacije na sobnoj temperaturi je odvajan krvni serum i čuvan na -20°C do momenta analize. Koncentracija ukupnih proteina u uzorcima krvnog seruma teladi je određivana kolorimetrijskom metodom, a koncentracija imunoglobulina G (IgG) radioimunodifuzionim testom (sRID). Koncentracija slobodnih aminokiselina je određivana u zbirnim uzorcima krvnog seruma (aspartat - Asp, glutamat - Glu, serin - Ser, histidin - His, glicin - Gly, treonin - Thr, alanin - Ala, prolin - Pro, tirozin - Tyr, arginin - Arg, valin - Val, metionin - Met, leucin - Leu, izoleucin - Ile, fenilalanin - Phe) metodom visoko precizne tečne hromatografije (HPLC, GBC Australia). Koncentracija ukupnih proteina u kolostrumu i kolostralnom mlečnom serumu je bila statistički značajno viša u prvom kolostrumu i snižena je između 50-75% u periodu 24-48 sati nakon partusa. Srednje vrednosti koncentracije IgG u krvnom serumu 6. i 16. sata nakon partusa bile su statistički značajno više kod tretirane grupe teladi u odnosu na kontrolnu grupu životinja (26±7:20±5 i 55±15: 42±13 g/L, p lt 0.05). Koncentracija ispitivanih slobodnih neesencijalnih i esencijalnih aminokiselina u zbirnim uzorcima krvnog seruma je povišena u svim vremenskim intervalima kod tretirane grupe teladi. Najveći stepen porasta koncentracije zabeležen je kod polarnih pozitivnih (Arg, His), polarnih neutralnih (Ser, Thr, Tyr) i nepolarnih neutralnih slobodnih aminokiselina (izuzev Met). Minimalni efekat oralnog tretmana zeolitom zabeležen je u slučaju koncentracije polarnih negativnih slobodnih aminokiselina (Asp i Glu) u zbirnim uzorcima krvnog seruma tretirane grupe teladi.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite
T1  - Slobodne aminokiseline u krvnom serumu kod novorođene teladi oralno tretirane zeolitom u periodu kolostralne ishrane
VL  - 60
IS  - 4
SP  - 411
EP  - 423
DO  - 10.2298/AVB1004411G
ER  - 

@article{
author = "Gvozdić, Dragan and Aleksić, Jelena and Fratrić, Natalija and Jakić-Dimić, Dobrila and Stojić, Velibor and Pavlović, V. and Pavlović, Miloš and Vakanjac, Slobodanka",
year = "2010",
abstract = "Oral zeolite treatement effects on the blood serum free amino acid pattern in newborn calves was investigated. The total number of 30 newborn Holstein calves of both sexes, weighting 35±3 kg (mean±SD), were immediately after parturition separated from their dams and placed in individual pens. Calves were divided in two experimental groups, 15 calves each. All calves were bottle-fed twice/ day (1.5 L/meal) during the first 48 hours after delivery, in 12 hour intervals, with their mother's first (during 24 hours postpartum) or second colostrum (at 24-48 hours postpartum), starting two hours after delivery. Zeolite suspension (20 mL, 25% suspension in distilled water) was added to every meal for treated calves. Colostrum samples were collected from six cows at 0-12h and 24h after delivery. Total and colostral whey protein concentrations were determined using the colorimetric method. Blood samples were taken from the jugular vein of calves at 6, 16, 30 and 40 hours after birth. After spontaneous coagulation at room temperature blood serum was separated and stored at -20°C until analyzed. Total protein concentration was determined by the colorimetric method. Blood serum immunoglobulin G (IgG) concentrations were determined using single radial immunodiffusion (sRID) plates. Pooled blood serum free amino acids (aspartic acid - Asp, glutamic acid - Glu, serine - Ser, histidine - His, glycine - Gly, threonine - Thr, alanine - Ala, proline - Pro, tyrosine - Tyr, arginine - Arg, valine - Val, methionine - Met, Leucine - Leu, Isoleucine - Ile, phenylalanine - Phe) were identified and quantified using high-performance liquid chromatography (HPLC, GBC Australia). Total and colostral whey protein concentrations were significantly higher in the first colostrum and decreased between 50-75% at 24-48 hours later on. Mean blood serum IgG concentration was significantly increased at 6 and 16 hours in the treated calves (26±7:20±5 and 55±15:42±13 g/L, p lt 0.05). Blood serum free amino acids (AA) first were separated at nonessential and essential AA (NEAA and EAA, respectively), both being increased at all time intervals after birth in treated calves. However, when the pooled blood serum free AA were clustered according to polarity and electrical charge and presented as relative values (% of the control group values) at the 6h there was a massive increase of polar positive (Arg, His), polar neutral (Ser, Thr, Tyr) and nonpolar neutral free AA (except Met). The minimal effect of oral zeolite treatment was on the negative polar blood serum free AA concentration (Asp and Glu)., U ovom radu su izneti rezultati ispitivanja uticaja oralnog tretmana zeolitom na koncentraciju slobodnih aminokiselina u krvnom serumu kod novorođene teladi. Ispitivanje je izvršeno na ukupno 30 novorođenih teladi, oba pola, prosečne telesne mase 35±3 kg (srednja vrednost±SD), koja su neposredno nakon partusa odvojena od majke i smeštena u individualne bokseve. Telad su podeljena u dve ogledne grupe sa po 15 životinja u svakoj grupi. Sva telad su hranjena dva puta dnevno (1.5 L/obroku) u toku prvih 48 sati nakon partusa kolostrumom njihovih majki, u intervalu od 12 sati, počevši od 2 sata nakon partusa. U toku prva 24 sata telad su hranjena prvim kolostrumom (skupljen u toku prvih 24 sata) dok su u sledeća 24 sata hranjena drugim kolostrumom (skupljen u periodu od 24-48 sati nakon partusa). Tretirana grupa teladi dobijala je sa svakim obrokom suspenziju zeolita (20 mL, 25% suspenzije zeolita u destilovanoj vodi). Uzorci kolostruma uzimani su od 6 krava u periodu od 0 - 12 sati i 24 sata nakon partusa i kolorimetrijskom metodom je određivana koncentracija proteina u kolostrumu i kolostralnom mlečnom serumu. Uzorci krvi od teladi uzimani su iz v. jugularis 6, 16, 30. i 40. sata nakon partusa, nakon spontane koagulacije na sobnoj temperaturi je odvajan krvni serum i čuvan na -20°C do momenta analize. Koncentracija ukupnih proteina u uzorcima krvnog seruma teladi je određivana kolorimetrijskom metodom, a koncentracija imunoglobulina G (IgG) radioimunodifuzionim testom (sRID). Koncentracija slobodnih aminokiselina je određivana u zbirnim uzorcima krvnog seruma (aspartat - Asp, glutamat - Glu, serin - Ser, histidin - His, glicin - Gly, treonin - Thr, alanin - Ala, prolin - Pro, tirozin - Tyr, arginin - Arg, valin - Val, metionin - Met, leucin - Leu, izoleucin - Ile, fenilalanin - Phe) metodom visoko precizne tečne hromatografije (HPLC, GBC Australia). Koncentracija ukupnih proteina u kolostrumu i kolostralnom mlečnom serumu je bila statistički značajno viša u prvom kolostrumu i snižena je između 50-75% u periodu 24-48 sati nakon partusa. Srednje vrednosti koncentracije IgG u krvnom serumu 6. i 16. sata nakon partusa bile su statistički značajno više kod tretirane grupe teladi u odnosu na kontrolnu grupu životinja (26±7:20±5 i 55±15: 42±13 g/L, p lt 0.05). Koncentracija ispitivanih slobodnih neesencijalnih i esencijalnih aminokiselina u zbirnim uzorcima krvnog seruma je povišena u svim vremenskim intervalima kod tretirane grupe teladi. Najveći stepen porasta koncentracije zabeležen je kod polarnih pozitivnih (Arg, His), polarnih neutralnih (Ser, Thr, Tyr) i nepolarnih neutralnih slobodnih aminokiselina (izuzev Met). Minimalni efekat oralnog tretmana zeolitom zabeležen je u slučaju koncentracije polarnih negativnih slobodnih aminokiselina (Asp i Glu) u zbirnim uzorcima krvnog seruma tretirane grupe teladi.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite, Slobodne aminokiseline u krvnom serumu kod novorođene teladi oralno tretirane zeolitom u periodu kolostralne ishrane",
volume = "60",
number = "4",
pages = "411-423",
doi = "10.2298/AVB1004411G"
}

Gvozdić, D., Aleksić, J., Fratrić, N., Jakić-Dimić, D., Stojić, V., Pavlović, V., Pavlović, M.,& Vakanjac, S.. (2010). Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(4), 411-423.
https://doi.org/10.2298/AVB1004411G

Gvozdić D, Aleksić J, Fratrić N, Jakić-Dimić D, Stojić V, Pavlović V, Pavlović M, Vakanjac S. Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite. in Acta Veterinaria-Beograd. 2010;60(4):411-423.
doi:10.2298/AVB1004411G .

Gvozdić, Dragan, Aleksić, Jelena, Fratrić, Natalija, Jakić-Dimić, Dobrila, Stojić, Velibor, Pavlović, V., Pavlović, Miloš, Vakanjac, Slobodanka, "Blood serum free amino acids pattern in newborn calves on colostral diet and orally treated with zeolite" in Acta Veterinaria-Beograd, 60, no. 4 (2010):411-423,
https://doi.org/10.2298/AVB1004411G . .

TY  - JOUR
AU  - Fratrić, Natalija
AU  - Ilić, Vesna
AU  - Milošević-Jovčić, Nadežda
AU  - Stojić, Velibor
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/668
AB  - Preruminant calves encounter numerous antigens, and formation of immune complexes is necessary for antigens elimination. The capability of immunologically immature calves to form immune complexes has not yet been studied in detail. For immune complexes studies, selective precipitation by PEG was performed, in combination with agarose gel electrophoresis and immunoelectrophoresis, with an aim to determine some properties of IgG, as constituents of immune complexes. In our previous work it was shown that the level of PEG precipitable immune complexes increased in the period from birth to 48-hours of life, decreased at day 10, increased in one month old animals, and after that, stayed unchanged until 4th month of age. Electrophoretic and immunoelectrophoretic analysis showed that until one month of age, calves' sera and PEG precipitates contained only one part of IgG molecules which corresponded to fast, anionic γ globulins. Although at the age of one month, preruminant calves' sera contained all molecular forms of IgG molecules present in the sera of adult cattle, only one part of serum IgG (fast, anionic IgG) was precipitated by PEG. In older calves, all molecular forms of serum IgG had the capacity to form PEG precipitable immune complexes. Presented data, as well as the results of our previous work, can be used as parameters with reference to physicochemical and immunochemical characteristics of IgG immune complexes of preruminant calves under pathological conditions.
AB  - Telad od rođenja dolazi u kontakt sa velikim brojem antigena za čije je efikasno uklanjanje neophodno formiranje imunokompleksa. Sposobnost ovih, još uvek imunološki nezrelih, životinja da formiraju iminokomplekse, kao i sastav njihovih konstituenata, nisu detaljno ispitivani. U ovom radu smo imunokomplekse iz seruma teladi izolovali precipitacijom polietilen glikolom (PEGom), a osobine IgG, kao potencijalnih konstituenata imunokompleksa, analizirali metodama elektroforeze i imunoelektroforeze. U našem prethodnom radu dokazali smo da se nivo PEG precipitabilnih imunokompleksa menja sa uzrastom teladi. On raste od rođenja do 48. sata, opada desetog dana, ponovo raste u prvom mesecu, nakon čega ostaje nepromenjen do četvrtog meseca starosti. Elekroforetske i imunoelektroforetske analize su ukazale da se u serumu i PEG precipitatima seruma teladi do mesec dana starosti nalazi samo jedna molekulska frakcija IgG koja odgovara brzim, anjonskim IgG. Iako se u serumima teladi već u prvom mesecu starosti nalaze sve molekulske forme IgG koje se nalaze i u serumu odraslih krava, samo jedan njihov deo (brzi, anjonski γ globulini) ima sposobnost da formira imune komplekse. Kod starijih teladi, kao i kod odraslih krava, sve molekulske forme serumskih IgG mogu da formiraju imune komplekse. Ovi rezultati, zajedno sa rezultatima našeg predhodnog rada, se mogu koristiti za dobijanje referentnog sistema vrednosti nivoa i sastava imunokompleksa kod zdravih teladi, a koji bi se mogao koristiti za određivanje nivoa i sastava imunokompleksa kod teladi u patološkim uslovima.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Electrophoretic and immunoelectrophoretic characteristics of IgG as a constituents of peg precipitable immune complexes in preruminant calves' sera
T1  - Elektroforetske i imunoelektroforetske karakteristike IgG kao konstituenata peg precipitabilnih imunokompleksa seruma kod teladi do četvrtog meseca starosti
VL  - 60
IS  - 2-3
SP  - 155
EP  - 164
DO  - 10.2298/AVB1003155F
ER  - 

@article{
author = "Fratrić, Natalija and Ilić, Vesna and Milošević-Jovčić, Nadežda and Stojić, Velibor",
year = "2010",
abstract = "Preruminant calves encounter numerous antigens, and formation of immune complexes is necessary for antigens elimination. The capability of immunologically immature calves to form immune complexes has not yet been studied in detail. For immune complexes studies, selective precipitation by PEG was performed, in combination with agarose gel electrophoresis and immunoelectrophoresis, with an aim to determine some properties of IgG, as constituents of immune complexes. In our previous work it was shown that the level of PEG precipitable immune complexes increased in the period from birth to 48-hours of life, decreased at day 10, increased in one month old animals, and after that, stayed unchanged until 4th month of age. Electrophoretic and immunoelectrophoretic analysis showed that until one month of age, calves' sera and PEG precipitates contained only one part of IgG molecules which corresponded to fast, anionic γ globulins. Although at the age of one month, preruminant calves' sera contained all molecular forms of IgG molecules present in the sera of adult cattle, only one part of serum IgG (fast, anionic IgG) was precipitated by PEG. In older calves, all molecular forms of serum IgG had the capacity to form PEG precipitable immune complexes. Presented data, as well as the results of our previous work, can be used as parameters with reference to physicochemical and immunochemical characteristics of IgG immune complexes of preruminant calves under pathological conditions., Telad od rođenja dolazi u kontakt sa velikim brojem antigena za čije je efikasno uklanjanje neophodno formiranje imunokompleksa. Sposobnost ovih, još uvek imunološki nezrelih, životinja da formiraju iminokomplekse, kao i sastav njihovih konstituenata, nisu detaljno ispitivani. U ovom radu smo imunokomplekse iz seruma teladi izolovali precipitacijom polietilen glikolom (PEGom), a osobine IgG, kao potencijalnih konstituenata imunokompleksa, analizirali metodama elektroforeze i imunoelektroforeze. U našem prethodnom radu dokazali smo da se nivo PEG precipitabilnih imunokompleksa menja sa uzrastom teladi. On raste od rođenja do 48. sata, opada desetog dana, ponovo raste u prvom mesecu, nakon čega ostaje nepromenjen do četvrtog meseca starosti. Elekroforetske i imunoelektroforetske analize su ukazale da se u serumu i PEG precipitatima seruma teladi do mesec dana starosti nalazi samo jedna molekulska frakcija IgG koja odgovara brzim, anjonskim IgG. Iako se u serumima teladi već u prvom mesecu starosti nalaze sve molekulske forme IgG koje se nalaze i u serumu odraslih krava, samo jedan njihov deo (brzi, anjonski γ globulini) ima sposobnost da formira imune komplekse. Kod starijih teladi, kao i kod odraslih krava, sve molekulske forme serumskih IgG mogu da formiraju imune komplekse. Ovi rezultati, zajedno sa rezultatima našeg predhodnog rada, se mogu koristiti za dobijanje referentnog sistema vrednosti nivoa i sastava imunokompleksa kod zdravih teladi, a koji bi se mogao koristiti za određivanje nivoa i sastava imunokompleksa kod teladi u patološkim uslovima.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Electrophoretic and immunoelectrophoretic characteristics of IgG as a constituents of peg precipitable immune complexes in preruminant calves' sera, Elektroforetske i imunoelektroforetske karakteristike IgG kao konstituenata peg precipitabilnih imunokompleksa seruma kod teladi do četvrtog meseca starosti",
volume = "60",
number = "2-3",
pages = "155-164",
doi = "10.2298/AVB1003155F"
}

Fratrić, N., Ilić, V., Milošević-Jovčić, N.,& Stojić, V.. (2010). Electrophoretic and immunoelectrophoretic characteristics of IgG as a constituents of peg precipitable immune complexes in preruminant calves' sera. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(2-3), 155-164.
https://doi.org/10.2298/AVB1003155F

Fratrić N, Ilić V, Milošević-Jovčić N, Stojić V. Electrophoretic and immunoelectrophoretic characteristics of IgG as a constituents of peg precipitable immune complexes in preruminant calves' sera. in Acta Veterinaria-Beograd. 2010;60(2-3):155-164.
doi:10.2298/AVB1003155F .

Fratrić, Natalija, Ilić, Vesna, Milošević-Jovčić, Nadežda, Stojić, Velibor, "Electrophoretic and immunoelectrophoretic characteristics of IgG as a constituents of peg precipitable immune complexes in preruminant calves' sera" in Acta Veterinaria-Beograd, 60, no. 2-3 (2010):155-164,
https://doi.org/10.2298/AVB1003155F . .

TY  - JOUR
AU  - Petrujkić, Branko
AU  - Šamanc, Horea
AU  - Adamović, Milan
AU  - Stojić, Velibor
AU  - Petrujkić, Tihomir
AU  - Grdović, Svetlana
AU  - Šefer, Dragan
AU  - Marković, Radmila
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/741
AB  - This trial was designed in order to evaluate the incidence of subacute rumen acidosis (SARA) during early lactation and to investigate the possibilities for its prevention by use of a buffering mineral mixture. On the beginning of the trial it was found that the pH value of rumen fluid in 4 animals was lower than normal (pH  lt  6.0) and that 20% of animals have had SARA. The control and the experimental group of cows were fed the same meal with exception of concentrated feed which in the experimental group contained the mineral mix with buffering activity in amount of 1%. Continuous addition of buffering mineral mixture in the amount of 1% in concentrated feed for early lactation cows successfully prevents SARA formation and leads to increased milk production, as well as increased milk fat and protein content.
PB  - Hokkaido Univ, Sapporo
T2  - Japanese Journal of Veterinary Research
T1  - Effects of feeding buffering mineral mixture on subacute rumen acidosis and some production traits in dairy cows
VL  - 58
IS  - 3-4
SP  - 171
EP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_741
ER  - 

@article{
author = "Petrujkić, Branko and Šamanc, Horea and Adamović, Milan and Stojić, Velibor and Petrujkić, Tihomir and Grdović, Svetlana and Šefer, Dragan and Marković, Radmila",
year = "2010",
abstract = "This trial was designed in order to evaluate the incidence of subacute rumen acidosis (SARA) during early lactation and to investigate the possibilities for its prevention by use of a buffering mineral mixture. On the beginning of the trial it was found that the pH value of rumen fluid in 4 animals was lower than normal (pH  lt  6.0) and that 20% of animals have had SARA. The control and the experimental group of cows were fed the same meal with exception of concentrated feed which in the experimental group contained the mineral mix with buffering activity in amount of 1%. Continuous addition of buffering mineral mixture in the amount of 1% in concentrated feed for early lactation cows successfully prevents SARA formation and leads to increased milk production, as well as increased milk fat and protein content.",
publisher = "Hokkaido Univ, Sapporo",
journal = "Japanese Journal of Veterinary Research",
title = "Effects of feeding buffering mineral mixture on subacute rumen acidosis and some production traits in dairy cows",
volume = "58",
number = "3-4",
pages = "171-177",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_741"
}

Petrujkić, B., Šamanc, H., Adamović, M., Stojić, V., Petrujkić, T., Grdović, S., Šefer, D.,& Marković, R.. (2010). Effects of feeding buffering mineral mixture on subacute rumen acidosis and some production traits in dairy cows. in Japanese Journal of Veterinary Research
Hokkaido Univ, Sapporo., 58(3-4), 171-177.
https://hdl.handle.net/21.15107/rcub_veterinar_741

Petrujkić B, Šamanc H, Adamović M, Stojić V, Petrujkić T, Grdović S, Šefer D, Marković R. Effects of feeding buffering mineral mixture on subacute rumen acidosis and some production traits in dairy cows. in Japanese Journal of Veterinary Research. 2010;58(3-4):171-177.
https://hdl.handle.net/21.15107/rcub_veterinar_741 .

Petrujkić, Branko, Šamanc, Horea, Adamović, Milan, Stojić, Velibor, Petrujkić, Tihomir, Grdović, Svetlana, Šefer, Dragan, Marković, Radmila, "Effects of feeding buffering mineral mixture on subacute rumen acidosis and some production traits in dairy cows" in Japanese Journal of Veterinary Research, 58, no. 3-4 (2010):171-177,
https://hdl.handle.net/21.15107/rcub_veterinar_741 .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Stojić, Velibor
AU  - Kirovski, Danijela
AU  - Jovanović, M.
AU  - Cernescu, H.
AU  - Vujanac, Ivan
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/740
AB  - Relationship between postpartal fatty liver and thyroid gland activity during the peripartal and mid dry periods was studied. Twenty one dry cows were chosen. Blood samples were obtained on days -30, -2, and +12 related to calving and analized for thyroxine (T4) and triiodothyronine (T3). A T3/T4 ratio was calculated. Liver tissue samples were taken 12 d after calving and tested for the lipid content. Cows were divided into three groups: mild (<20% fat), moderate (20 to 30%), or severe fatty liver (>30%). Cows, that were affected with severe fatty liver, were hypothyroid prior to development of the condition due to lower T4 concentrations, and had significantly lower concentration of T3 and higher T3/T4 ratios than cows with mild and moderate fatty liver. Thus, hypothyroid state during mid-dry period may be an early indicator of postpartal fatty liver and may provoke T3/T4 ratio increase in this group of cows.
PB  - Hindawi Limited
T2  - Journal of Thyroid Research
T1  - Thyroid hormones concentrations during the mid-dry period: An early indicator of fatty liver in Holstein-Friesian dairy cows
VL  - 2010
SP  - 897602
DO  - 10.4061/2010/897602
ER  - 

@article{
author = "Šamanc, Horea and Stojić, Velibor and Kirovski, Danijela and Jovanović, M. and Cernescu, H. and Vujanac, Ivan",
year = "2010",
abstract = "Relationship between postpartal fatty liver and thyroid gland activity during the peripartal and mid dry periods was studied. Twenty one dry cows were chosen. Blood samples were obtained on days -30, -2, and +12 related to calving and analized for thyroxine (T4) and triiodothyronine (T3). A T3/T4 ratio was calculated. Liver tissue samples were taken 12 d after calving and tested for the lipid content. Cows were divided into three groups: mild (<20% fat), moderate (20 to 30%), or severe fatty liver (>30%). Cows, that were affected with severe fatty liver, were hypothyroid prior to development of the condition due to lower T4 concentrations, and had significantly lower concentration of T3 and higher T3/T4 ratios than cows with mild and moderate fatty liver. Thus, hypothyroid state during mid-dry period may be an early indicator of postpartal fatty liver and may provoke T3/T4 ratio increase in this group of cows.",
publisher = "Hindawi Limited",
journal = "Journal of Thyroid Research",
title = "Thyroid hormones concentrations during the mid-dry period: An early indicator of fatty liver in Holstein-Friesian dairy cows",
volume = "2010",
pages = "897602",
doi = "10.4061/2010/897602"
}

Šamanc, H., Stojić, V., Kirovski, D., Jovanović, M., Cernescu, H.,& Vujanac, I.. (2010). Thyroid hormones concentrations during the mid-dry period: An early indicator of fatty liver in Holstein-Friesian dairy cows. in Journal of Thyroid Research
Hindawi Limited., 2010, 897602.
https://doi.org/10.4061/2010/897602

Šamanc H, Stojić V, Kirovski D, Jovanović M, Cernescu H, Vujanac I. Thyroid hormones concentrations during the mid-dry period: An early indicator of fatty liver in Holstein-Friesian dairy cows. in Journal of Thyroid Research. 2010;2010:897602.
doi:10.4061/2010/897602 .

Šamanc, Horea, Stojić, Velibor, Kirovski, Danijela, Jovanović, M., Cernescu, H., Vujanac, Ivan, "Thyroid hormones concentrations during the mid-dry period: An early indicator of fatty liver in Holstein-Friesian dairy cows" in Journal of Thyroid Research, 2010 (2010):897602,
https://doi.org/10.4061/2010/897602 . .

TY  - JOUR
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Aleksić, Nevenka
AU  - Stojić, Velibor
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/683
AB  - Grooming behavior is considered an important defensive mechanism of honey bees against Varroa mites. The aim of this study was to reveal whether grooming behavior is a useful criterion in breeding of Varroa-tolerant bees. To obtain a reliable evaluation the environmental influences were excluded. The degree of grooming potential was estimated by the percentage of damaged mites in the total number of fallen mites. The heritability of grooming behavior throughout the three consecutive generations of queens was assessed by mother-daughter regression method. Among unselected queens, expressed grooming behavior was recorded only in colonies with F1 queens (36.27%), but not in colonies with P queens and F2 queens (33.69%, 31.66%, respectively). Significant differences in grooming behavior were found between colonies of P and F1 queens (p lt 0.001), and between colonies of P and F2 queens (p lt 0.05). However, all of the three generations of selected queens showed expressed grooming behavior (37.99%, 39.42% and 38.58% in Ps, F1s and F2s, respectively) without significant (p>0.05) difference among them. Nevertheless, the relatively low heritability of grooming behavior in the three generations of queens examined (h2yx=0.49±0.02; h2zx=0.18±0.01; h2zy=0.16±0.01) indicate that breeding colonies for grooming behavior only cannot be advised to beekeepers whose aim is to breed bees highly tolerant to Varroa mites.
AB  - Negovateljsko ponašanje se smatra značajnim mehanizmom odbrane pčela od Varroa krpelja. Cilj ovog rada je bilo ispitivanje negovateljskog ponašanja, procena njegove heritabilnosti i mogućnosti povećanja ekspresije te osobine putem selekcije. Radi dobijanja pouzdanih rezultata korišćenja je metodologija kojom se uticaj spoljašnjih faktora isključuje. Ispoljenost negovateljskog ponašanja procenjivana je na osnovu procenta oštećenih u ukupnom broju otpalih krpelja. Heritabilnost negovateljskog ponašanja praćena na kroz generacije matica i procenjivana metodom regresije majka-ćerka. Među neselekcionisanim maticama, negovateljsko ponašanje bilo je izraženo samo kod matica F1 generacije (36,27%), ali ne i kod P (33,69%) i F2 generacije (31,66%). Statistički značajne razlike u negovateljskom ponašanju zabeležene su između društava P i F1 matica (p lt 0,001) i između društava P i F2 matica (p lt 0,05). Međutim, selekcionisane matice sve tri generacije (Ps, F1s, F2s) su imale izraženo negovateljsko ponašanje (37,99%, 39,42% i 38,58%) bez statistički značajnih (p>0,05) razlika među njima. Ipak, nizak koeficijent heritabilnosti praćene osobine (h2yx=0,49±0,02; h2zx=0,18±0,01; h2zy=0,16±0,01) ukazuje da se pčelarima ne može preporučiti selekcija pčela samo na negovateljsko ponašanje ako je njihov cilj uzgoj pčelinjih zajednica povećane otpornosti na Varroa krpelje.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Heritability of grooming behaviour in grey honey bees (Apis mellifera Carnica)
T1  - Heritabilnost negovateljskog ponašanja sive medonosne pčele (Apis mellifera Carnica)
VL  - 60
IS  - 2-3
SP  - 313
EP  - 323
DO  - 10.2298/AVB1003313S
ER  - 

@article{
author = "Stanimirović, Zoran and Stevanović, Jevrosima and Aleksić, Nevenka and Stojić, Velibor",
year = "2010",
abstract = "Grooming behavior is considered an important defensive mechanism of honey bees against Varroa mites. The aim of this study was to reveal whether grooming behavior is a useful criterion in breeding of Varroa-tolerant bees. To obtain a reliable evaluation the environmental influences were excluded. The degree of grooming potential was estimated by the percentage of damaged mites in the total number of fallen mites. The heritability of grooming behavior throughout the three consecutive generations of queens was assessed by mother-daughter regression method. Among unselected queens, expressed grooming behavior was recorded only in colonies with F1 queens (36.27%), but not in colonies with P queens and F2 queens (33.69%, 31.66%, respectively). Significant differences in grooming behavior were found between colonies of P and F1 queens (p lt 0.001), and between colonies of P and F2 queens (p lt 0.05). However, all of the three generations of selected queens showed expressed grooming behavior (37.99%, 39.42% and 38.58% in Ps, F1s and F2s, respectively) without significant (p>0.05) difference among them. Nevertheless, the relatively low heritability of grooming behavior in the three generations of queens examined (h2yx=0.49±0.02; h2zx=0.18±0.01; h2zy=0.16±0.01) indicate that breeding colonies for grooming behavior only cannot be advised to beekeepers whose aim is to breed bees highly tolerant to Varroa mites., Negovateljsko ponašanje se smatra značajnim mehanizmom odbrane pčela od Varroa krpelja. Cilj ovog rada je bilo ispitivanje negovateljskog ponašanja, procena njegove heritabilnosti i mogućnosti povećanja ekspresije te osobine putem selekcije. Radi dobijanja pouzdanih rezultata korišćenja je metodologija kojom se uticaj spoljašnjih faktora isključuje. Ispoljenost negovateljskog ponašanja procenjivana je na osnovu procenta oštećenih u ukupnom broju otpalih krpelja. Heritabilnost negovateljskog ponašanja praćena na kroz generacije matica i procenjivana metodom regresije majka-ćerka. Među neselekcionisanim maticama, negovateljsko ponašanje bilo je izraženo samo kod matica F1 generacije (36,27%), ali ne i kod P (33,69%) i F2 generacije (31,66%). Statistički značajne razlike u negovateljskom ponašanju zabeležene su između društava P i F1 matica (p lt 0,001) i između društava P i F2 matica (p lt 0,05). Međutim, selekcionisane matice sve tri generacije (Ps, F1s, F2s) su imale izraženo negovateljsko ponašanje (37,99%, 39,42% i 38,58%) bez statistički značajnih (p>0,05) razlika među njima. Ipak, nizak koeficijent heritabilnosti praćene osobine (h2yx=0,49±0,02; h2zx=0,18±0,01; h2zy=0,16±0,01) ukazuje da se pčelarima ne može preporučiti selekcija pčela samo na negovateljsko ponašanje ako je njihov cilj uzgoj pčelinjih zajednica povećane otpornosti na Varroa krpelje.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Heritability of grooming behaviour in grey honey bees (Apis mellifera Carnica), Heritabilnost negovateljskog ponašanja sive medonosne pčele (Apis mellifera Carnica)",
volume = "60",
number = "2-3",
pages = "313-323",
doi = "10.2298/AVB1003313S"
}

Stanimirović, Z., Stevanović, J., Aleksić, N.,& Stojić, V.. (2010). Heritability of grooming behaviour in grey honey bees (Apis mellifera Carnica). in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(2-3), 313-323.
https://doi.org/10.2298/AVB1003313S

Stanimirović Z, Stevanović J, Aleksić N, Stojić V. Heritability of grooming behaviour in grey honey bees (Apis mellifera Carnica). in Acta Veterinaria-Beograd. 2010;60(2-3):313-323.
doi:10.2298/AVB1003313S .

Stanimirović, Zoran, Stevanović, Jevrosima, Aleksić, Nevenka, Stojić, Velibor, "Heritability of grooming behaviour in grey honey bees (Apis mellifera Carnica)" in Acta Veterinaria-Beograd, 60, no. 2-3 (2010):313-323,
https://doi.org/10.2298/AVB1003313S . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Milić, Nenad
AU  - Stojić, Velibor
AU  - Knežević, Dejan
AU  - Vujanac, Ivan
AU  - Dimitrijević, Blagoje
AU  - Nišavić, Jakov
AU  - Radojičić, Marina
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/648
AB  - A total of 92 samples of bovine blood serum were examined for the presence of antibodies against the bovine respiratory syncytial virus using indirect immunoenzyme probe - iELISA. Specific antibodies against the bovine respiratory syncytial virus (BRSV) were established in 46, or 50% blood serum samples. Investigations of the 92 blood serum samples of cattle for the presence of antibodies against the parainfluenza virus 3 (PI 3), revealed their presence in 77, or 83.69% of the samples, and the presence of antibodies against the bovine herpesvirus 1 (BHV 1) was established in 19, or 20.65% of the samples.
AB  - Ukupno je ispitano 92 uzorka krvnog seruma junadi na prisustvo antitela protiv goveđeg respiratornog sincicijalnog virusa primenom indirektne imunoenzimske probe - iELISA. Kod 46, odnosno 50% uzoraka krvnog seruma ustanovljena su specifična antitela protiv goveđeg respiratornog sincicijalnog virusa (BRSV). Ispitivanjem 92 uzorka krvnog seruma goveda na prisustvo antitela protiv virusa parainfluence 3 (PI 3) ustanovljeno je njihovo prisustvo kod 77, odnosno 83,69% uzoraka, a prisustvo antitela protiv goveđeg herpesvirusa 1 (BHV 1) ustanovljeno je kod 19, odnosno 20,65% uzoraka.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Establishing presence of antibodies against bovine respiratory syncytial virus (BRSV), parainfluenza virus 3 (PI3) and bovine herpesvirus 1 (BHV 1) in blood serum of cattle using indirect immunoenzyme probe
T1  - Utvrđivanje prisustva antitela protiv goveđeg respiratornog sincicijalnog virusa (BRSV), virusa parainfluence 3 (PI3) i goveđeg herpesvirusa 1 (BHV 1) u krvnom serumu junadi primenom indirektne imunoenzimske probe
VL  - 63
IS  - 3-4
SP  - 145
EP  - 152
DO  - 10.2298/VETGL0904145S
ER  - 

@article{
author = "Šamanc, Horea and Milić, Nenad and Stojić, Velibor and Knežević, Dejan and Vujanac, Ivan and Dimitrijević, Blagoje and Nišavić, Jakov and Radojičić, Marina",
year = "2009",
abstract = "A total of 92 samples of bovine blood serum were examined for the presence of antibodies against the bovine respiratory syncytial virus using indirect immunoenzyme probe - iELISA. Specific antibodies against the bovine respiratory syncytial virus (BRSV) were established in 46, or 50% blood serum samples. Investigations of the 92 blood serum samples of cattle for the presence of antibodies against the parainfluenza virus 3 (PI 3), revealed their presence in 77, or 83.69% of the samples, and the presence of antibodies against the bovine herpesvirus 1 (BHV 1) was established in 19, or 20.65% of the samples., Ukupno je ispitano 92 uzorka krvnog seruma junadi na prisustvo antitela protiv goveđeg respiratornog sincicijalnog virusa primenom indirektne imunoenzimske probe - iELISA. Kod 46, odnosno 50% uzoraka krvnog seruma ustanovljena su specifična antitela protiv goveđeg respiratornog sincicijalnog virusa (BRSV). Ispitivanjem 92 uzorka krvnog seruma goveda na prisustvo antitela protiv virusa parainfluence 3 (PI 3) ustanovljeno je njihovo prisustvo kod 77, odnosno 83,69% uzoraka, a prisustvo antitela protiv goveđeg herpesvirusa 1 (BHV 1) ustanovljeno je kod 19, odnosno 20,65% uzoraka.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Establishing presence of antibodies against bovine respiratory syncytial virus (BRSV), parainfluenza virus 3 (PI3) and bovine herpesvirus 1 (BHV 1) in blood serum of cattle using indirect immunoenzyme probe, Utvrđivanje prisustva antitela protiv goveđeg respiratornog sincicijalnog virusa (BRSV), virusa parainfluence 3 (PI3) i goveđeg herpesvirusa 1 (BHV 1) u krvnom serumu junadi primenom indirektne imunoenzimske probe",
volume = "63",
number = "3-4",
pages = "145-152",
doi = "10.2298/VETGL0904145S"
}

Šamanc, H., Milić, N., Stojić, V., Knežević, D., Vujanac, I., Dimitrijević, B., Nišavić, J.,& Radojičić, M.. (2009). Establishing presence of antibodies against bovine respiratory syncytial virus (BRSV), parainfluenza virus 3 (PI3) and bovine herpesvirus 1 (BHV 1) in blood serum of cattle using indirect immunoenzyme probe. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(3-4), 145-152.
https://doi.org/10.2298/VETGL0904145S

Šamanc H, Milić N, Stojić V, Knežević D, Vujanac I, Dimitrijević B, Nišavić J, Radojičić M. Establishing presence of antibodies against bovine respiratory syncytial virus (BRSV), parainfluenza virus 3 (PI3) and bovine herpesvirus 1 (BHV 1) in blood serum of cattle using indirect immunoenzyme probe. in Veterinarski Glasnik. 2009;63(3-4):145-152.
doi:10.2298/VETGL0904145S .

Šamanc, Horea, Milić, Nenad, Stojić, Velibor, Knežević, Dejan, Vujanac, Ivan, Dimitrijević, Blagoje, Nišavić, Jakov, Radojičić, Marina, "Establishing presence of antibodies against bovine respiratory syncytial virus (BRSV), parainfluenza virus 3 (PI3) and bovine herpesvirus 1 (BHV 1) in blood serum of cattle using indirect immunoenzyme probe" in Veterinarski Glasnik, 63, no. 3-4 (2009):145-152,
https://doi.org/10.2298/VETGL0904145S . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Dimitrijević, Vladimir
AU  - Stojić, Velibor
AU  - Fratrić, Natalija
AU  - Lazarević, Miodrag
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/573
AB  - The polymorphism of 11 microsatellite DNA markers (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824) was analyzed in order to assess the usefulness of these markers for pedigree verification in the Serbian population of Simmental cattle. A total of 86 animals were involved in the study. Genomic DNA was extracted from sperm and blood and amplified in a multiplex PCR using primers from the commercial 'StockMarks for Cattle® Bovine Genotyping Kit'. Analyses were done with automated DNA sizing technology. A total of 92 alleles were detected. The mean number of alleles (nA) per locus was 8.364, the mean value of polymorphism information content (PIC) was 0.73, and the mean frequency of the most frequent allele (FNA) was 0.372. The most polymorphic microsatellite loci were: TGLA53 (14 alleles, PIC=0.88, FNA=0.235), TGLA227 (11 alleles, PIC=0.82, FNA=0.274), INRA23 (11 alleles, PIC=0.86, FNA=0.167), BM2113 (9 alleles, PIC=0.84, FNA=0.330). Both combined power of discrimination (CPD) and combined power of exclusion (CPE) for the whole set of studied markers were 0.999. In conclusion, the polymorphism of 11 microsatellite DNA markers observed in this study indicate its usefulness for paternity testing and pedigree verification in Simmental cattle from Serbia.
AB  - U ovom radu analiziran je polimorfizam 11 mikrosatelitskih DNK markera (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824) u cilju procene upotrebljivosti tih markera u verifikaciji pedigrea Simentalskih goveda u Srbiji. Analizirano je ukupno 86 grla. Genomska DNK je izolovana iz sperme i krvi. Za amplifikaciju je primenjen multiplex PCR korišćenjem prajmera komercijalnog seta 'StockMarks for Cattle® Bovine Genotyping Kit'. Pri analizi rezultata korišćena je tehnologija automatizovanog određivanja veličine DNA fragmenata. Ukupan broj detektovanih alela bio je 92. Srednja vrednost broja alela (nA) po lokusu iznosila je 8,364, srednja vrednost sadržaja polimorfne informacije (PIC) bila je 0,73, dok je učestalosti najčešćeg alela (FNA) u proseku iznosila 0,372. Najveća polimorfnost uočena je kod lokusa TGLA53 (14 alela, PIC=0,88, FNA=0,235), TGLA227 (11 alela, PIC=0,82, FNA=0,274), INRA23 (11 alela, PIC=0,86, FNA=0,167), BM2113 (9 alela, PIC=0,84, FNA= 0,330). Moć diskriminacije i moć isključenja celog seta proučavanih markera iznosila je 0,999. Polimorfizam uočen kod 11 mikrosatelitskih DNK markera u ovom radu ukazuje na mogućnost njihove primene u testiranju očinstva i verifikaciji pedigrea Simentalskih goveda u Srbiji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Microsatellite DNA polymorphism and its usefulness for pedigree verification in Simmental cattle from Serbia
T1  - Polimorfizam DNK mikrosatelita i mogućnost njihove primene u verifikaciji pedigrea simentalskih goveda u Srbiji
VL  - 59
IS  - 5-6
SP  - 621
EP  - 631
DO  - 10.2298/AVB0906621S
ER  - 

@article{
author = "Stevanović, Jevrosima and Stanimirović, Zoran and Dimitrijević, Vladimir and Stojić, Velibor and Fratrić, Natalija and Lazarević, Miodrag",
year = "2009",
abstract = "The polymorphism of 11 microsatellite DNA markers (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824) was analyzed in order to assess the usefulness of these markers for pedigree verification in the Serbian population of Simmental cattle. A total of 86 animals were involved in the study. Genomic DNA was extracted from sperm and blood and amplified in a multiplex PCR using primers from the commercial 'StockMarks for Cattle® Bovine Genotyping Kit'. Analyses were done with automated DNA sizing technology. A total of 92 alleles were detected. The mean number of alleles (nA) per locus was 8.364, the mean value of polymorphism information content (PIC) was 0.73, and the mean frequency of the most frequent allele (FNA) was 0.372. The most polymorphic microsatellite loci were: TGLA53 (14 alleles, PIC=0.88, FNA=0.235), TGLA227 (11 alleles, PIC=0.82, FNA=0.274), INRA23 (11 alleles, PIC=0.86, FNA=0.167), BM2113 (9 alleles, PIC=0.84, FNA=0.330). Both combined power of discrimination (CPD) and combined power of exclusion (CPE) for the whole set of studied markers were 0.999. In conclusion, the polymorphism of 11 microsatellite DNA markers observed in this study indicate its usefulness for paternity testing and pedigree verification in Simmental cattle from Serbia., U ovom radu analiziran je polimorfizam 11 mikrosatelitskih DNK markera (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824) u cilju procene upotrebljivosti tih markera u verifikaciji pedigrea Simentalskih goveda u Srbiji. Analizirano je ukupno 86 grla. Genomska DNK je izolovana iz sperme i krvi. Za amplifikaciju je primenjen multiplex PCR korišćenjem prajmera komercijalnog seta 'StockMarks for Cattle® Bovine Genotyping Kit'. Pri analizi rezultata korišćena je tehnologija automatizovanog određivanja veličine DNA fragmenata. Ukupan broj detektovanih alela bio je 92. Srednja vrednost broja alela (nA) po lokusu iznosila je 8,364, srednja vrednost sadržaja polimorfne informacije (PIC) bila je 0,73, dok je učestalosti najčešćeg alela (FNA) u proseku iznosila 0,372. Najveća polimorfnost uočena je kod lokusa TGLA53 (14 alela, PIC=0,88, FNA=0,235), TGLA227 (11 alela, PIC=0,82, FNA=0,274), INRA23 (11 alela, PIC=0,86, FNA=0,167), BM2113 (9 alela, PIC=0,84, FNA= 0,330). Moć diskriminacije i moć isključenja celog seta proučavanih markera iznosila je 0,999. Polimorfizam uočen kod 11 mikrosatelitskih DNK markera u ovom radu ukazuje na mogućnost njihove primene u testiranju očinstva i verifikaciji pedigrea Simentalskih goveda u Srbiji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Microsatellite DNA polymorphism and its usefulness for pedigree verification in Simmental cattle from Serbia, Polimorfizam DNK mikrosatelita i mogućnost njihove primene u verifikaciji pedigrea simentalskih goveda u Srbiji",
volume = "59",
number = "5-6",
pages = "621-631",
doi = "10.2298/AVB0906621S"
}

Stevanović, J., Stanimirović, Z., Dimitrijević, V., Stojić, V., Fratrić, N.,& Lazarević, M.. (2009). Microsatellite DNA polymorphism and its usefulness for pedigree verification in Simmental cattle from Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(5-6), 621-631.
https://doi.org/10.2298/AVB0906621S

Stevanović J, Stanimirović Z, Dimitrijević V, Stojić V, Fratrić N, Lazarević M. Microsatellite DNA polymorphism and its usefulness for pedigree verification in Simmental cattle from Serbia. in Acta Veterinaria-Beograd. 2009;59(5-6):621-631.
doi:10.2298/AVB0906621S .

Stevanović, Jevrosima, Stanimirović, Zoran, Dimitrijević, Vladimir, Stojić, Velibor, Fratrić, Natalija, Lazarević, Miodrag, "Microsatellite DNA polymorphism and its usefulness for pedigree verification in Simmental cattle from Serbia" in Acta Veterinaria-Beograd, 59, no. 5-6 (2009):621-631,
https://doi.org/10.2298/AVB0906621S . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Stojić, Velibor
AU  - Kirovski, Danijela
AU  - Pušić, M.
AU  - Jakić-Dimić, Dobrila
AU  - Vujanac, Ivan
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/655
AB  - The concentration of biologically active molecules in colostrum is strongly related to the metabolic and endocrine status of the cow during the early postpartum period. The aim of this study was to determine concentrations of insulin like growth factor-I (IGF-I), triiodothyronine (T3) and thyroxine (T4) in mammary gland secretion during the late dry and colostral period and its possible association with the energy status of the cows as represented by blood glucose concentration. Ten healthy Holstein-Friesian dairy cows were involved in this study. Two samples of colostrum were taken from each animal: one between 19 and 53 hours (mean [sd] 32.10 [9.47] hours) before calving, and the second 2 hours after calving. Concentrations of IGF-I, T3 and T4 tended to decrease at the start of lactation but the changes were not statistically significant. Linear regression of colostral IGF-I and protein concentration before calving revealed a positive relationship (R2 = 0.668, p lt 0.01) before calving but not post partum (R2 = 0.348, p>0.05). Blood glucose concentration was 2.88 ± 0.32 mmol/L before and 2.28 ± 0.29 mmol/L after calving and the decrease was statistically significant (p lt 0.001). Blood glucose levels after calving were positively correlated with colostral IGF-I concentration after calving (R2 = 0.695, p lt 0.01), but not before parturition (R2 = 0.383, p>0.05).
AB  - Koncentracija biološki aktivnih molekula u kolostrumu je u visokoj korelaciji sa metaboličkim i endokrinim statusom krava tokom peripartalnog perioda. Cilj ovog rada je bio da se odrede koncentracije insulinu sličnog faktora rasta-I (IGF-I), trijodtironina (T3) i tiroksina (T4) u sekretu mlečne žlezde tokom perioda kasnog zasušenja i ranog kolostralnog perioda, kao i njihova moguća korelacija sa energetskim statusom krava izraženim kroz koncentraciju glukoze u krvi. Ispitivanje je izvedeno na deset zdravih krava holštajn-frizijske rase. Koncentracije IGF-I, T3 i T4 su imale tendenciju pada na početku laktacije, ali promene nisu bile statistički značajne. Između koncentracije IGF-I i proteina u kolostrumu postojala je pozitivna korelacija (R2 = 0,668, p lt 0,01) pre teljenja, ali ne i posle teljenja (R2 = 0,348, p>0,05). Koncentracija glukoze u krvi je bila 2,88 ± 0,32 mmol/l pre i 2,28 ± 0,29 mmol/l posle teljenja, a smanjenje koncentracije je bilo statistički značajno (p lt 0,001). Koncentracija glukoze u krvi posle teljenja je bila u pozitivnoj korelaciji sa koncentracijom IGF-I u kolostrumu posle teljenja (R2 = 0,695, p lt 0,01), ali ne i pre teljenja (R2 = 0,383, p>0,05).
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving
T1  - Koncentracija insulinu sličnog faktora rasta-I (IGF-I) i tireoidnih hormona u kolostrumu krava holštajn-frizijske rase pre i posle teljenja
VL  - 59
IS  - 1
SP  - 17
EP  - 23
DO  - 10.2298/AVB0901017S
ER  - 

@article{
author = "Šamanc, Horea and Stojić, Velibor and Kirovski, Danijela and Pušić, M. and Jakić-Dimić, Dobrila and Vujanac, Ivan",
year = "2009",
abstract = "The concentration of biologically active molecules in colostrum is strongly related to the metabolic and endocrine status of the cow during the early postpartum period. The aim of this study was to determine concentrations of insulin like growth factor-I (IGF-I), triiodothyronine (T3) and thyroxine (T4) in mammary gland secretion during the late dry and colostral period and its possible association with the energy status of the cows as represented by blood glucose concentration. Ten healthy Holstein-Friesian dairy cows were involved in this study. Two samples of colostrum were taken from each animal: one between 19 and 53 hours (mean [sd] 32.10 [9.47] hours) before calving, and the second 2 hours after calving. Concentrations of IGF-I, T3 and T4 tended to decrease at the start of lactation but the changes were not statistically significant. Linear regression of colostral IGF-I and protein concentration before calving revealed a positive relationship (R2 = 0.668, p lt 0.01) before calving but not post partum (R2 = 0.348, p>0.05). Blood glucose concentration was 2.88 ± 0.32 mmol/L before and 2.28 ± 0.29 mmol/L after calving and the decrease was statistically significant (p lt 0.001). Blood glucose levels after calving were positively correlated with colostral IGF-I concentration after calving (R2 = 0.695, p lt 0.01), but not before parturition (R2 = 0.383, p>0.05)., Koncentracija biološki aktivnih molekula u kolostrumu je u visokoj korelaciji sa metaboličkim i endokrinim statusom krava tokom peripartalnog perioda. Cilj ovog rada je bio da se odrede koncentracije insulinu sličnog faktora rasta-I (IGF-I), trijodtironina (T3) i tiroksina (T4) u sekretu mlečne žlezde tokom perioda kasnog zasušenja i ranog kolostralnog perioda, kao i njihova moguća korelacija sa energetskim statusom krava izraženim kroz koncentraciju glukoze u krvi. Ispitivanje je izvedeno na deset zdravih krava holštajn-frizijske rase. Koncentracije IGF-I, T3 i T4 su imale tendenciju pada na početku laktacije, ali promene nisu bile statistički značajne. Između koncentracije IGF-I i proteina u kolostrumu postojala je pozitivna korelacija (R2 = 0,668, p lt 0,01) pre teljenja, ali ne i posle teljenja (R2 = 0,348, p>0,05). Koncentracija glukoze u krvi je bila 2,88 ± 0,32 mmol/l pre i 2,28 ± 0,29 mmol/l posle teljenja, a smanjenje koncentracije je bilo statistički značajno (p lt 0,001). Koncentracija glukoze u krvi posle teljenja je bila u pozitivnoj korelaciji sa koncentracijom IGF-I u kolostrumu posle teljenja (R2 = 0,695, p lt 0,01), ali ne i pre teljenja (R2 = 0,383, p>0,05).",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving, Koncentracija insulinu sličnog faktora rasta-I (IGF-I) i tireoidnih hormona u kolostrumu krava holštajn-frizijske rase pre i posle teljenja",
volume = "59",
number = "1",
pages = "17-23",
doi = "10.2298/AVB0901017S"
}

Šamanc, H., Stojić, V., Kirovski, D., Pušić, M., Jakić-Dimić, D.,& Vujanac, I.. (2009). Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(1), 17-23.
https://doi.org/10.2298/AVB0901017S

Šamanc H, Stojić V, Kirovski D, Pušić M, Jakić-Dimić D, Vujanac I. Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving. in Acta Veterinaria-Beograd. 2009;59(1):17-23.
doi:10.2298/AVB0901017S .

Šamanc, Horea, Stojić, Velibor, Kirovski, Danijela, Pušić, M., Jakić-Dimić, Dobrila, Vujanac, Ivan, "Insulin-like growth factor-I (IGF-I) and thyroid hormone concentrations in colostrum of Holstein-Friesian cows before and after calving" in Acta Veterinaria-Beograd, 59, no. 1 (2009):17-23,
https://doi.org/10.2298/AVB0901017S . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Stojić, Velibor
AU  - Kirovski, Danijela
AU  - Pudlo, Pavle
AU  - Vujanac, Ivan
PY  - 2009
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/632
AB  - The aim of this study was to examine the secretory capacity of endocrine pancreas beta cells in healthy cows and cows suffering from left displaced abomasum (LDA) by determination of glucose and insulin concentrations in the peripheral circulation during glucose tolerance test (GTT). A total of twenty healthy cows (Control group) and twenty cows suffering from left abomasal displacement (Experimental group) were chosen for this study. Cows in the control group were exposed to GTT once, while cows in the experimental group were exposed on the day, day 3 and day 6 after the diagnosis of LDA, as well as 3 days after surgical treatment and reposition of the abomasum. Initial blood glucose concentrations in healthy cows were significantly lower than in the cows suffering from LDA only on day 3 (P lt 0.05) and day 6 (P lt 0.01) after the disease was diagnosed. All the glucose values determined both in healthy and diseased cows at 30. minutes after the start of glucose infusion were much higher than physiological values. Regardless to the decline of glucose levels from 60 to 180 minutes after start of glucose infusion, hyperglicemia maintained in both healthy cows and cows suffering from LDA. Compared to healthy cows the rate of decline was slower in cows suffering from LDA at the day of diagnosis, as well as 3 and 6 days after the diagnosis. At the end of the experiment, 240 minutes after the start of the infusion, glucose concentrations were significantly higher in diseased cows on the day of diagnosis and day 3 and 6 after diagnosis than in the control group (P lt 0.001, respectively) and in diseased cows 3 days after surgery (P lt 0.001, respectively). Mean initial concentrations of serum insulin were significantly higher in healthy cows than in cows suffering from LDA only on day 6 after diagnosis. From 30 to 240 minutes after the start of glucose infusion, insulinemic responses to intravenous glucose administation were statistically higher in healthy cows than in experimental cows on the day of diagnosis, day 3 and day 6 after diagnosis, as well as 3 days after surgery. On the day of diagnosis and three days after, insulinemia decreased to initial levels 180 minutes after the start of the infusion. Six days after diagnosis, insulinemia decreased to initial values at 120 minutes after the start of the glucose infusion. Three days after surgery, insulinemia decrased to values similar to initial at 240 minutes after the start of the infusion. Glucose tolerance test results showed that the best validation of pancreas endocrine function was between 60 and 120 minutes, as well as 180 and 240 minutes after the glucose infusion started. Our results also confirmed that cows suffering from LDA are in the stage of pancreatic endocrine disfunction and that the rate of this disfunction was higher when the disease lasted longer. The highest rate of pancreatic endocrine disfunction was observed 6 days after diagnosis. On day 3 after surgical treatment and reposition of the abomasum, glucose tolerance test results showed that the pancreatic endocrine function recovered.
AB  - Cilj ovog rada bio je da se, određivanjem koncentracije glukoze i insulina u krvi krava tokom testa opterećenja glukozom, ispita sekretorni kapacitet beta ćelija endokrinog pankreasa kod zdravih krava i krava obolelih od dislokacije sirišta na levo. U ogled je uključeno 20 zdravih krava (kontrolna grupa) i 20 krava obolelih od dislokacije sirišta na levo (eksperimentalna grupa). Kod zdravih krava opterećenje glukozom je izvršeno jednom, dok je kod obolelih krava ovaj test izveden na dan dijagnostikovanja oboljenja, 3 i 6 dana posle, kao i 3 dana nakon hirurške intervencije i repozicije obolelog organa. Bazalna koncentracija glukoze, određena neposredno pre početka infuzije, je kod zdravih krava bila značajno niža nego kod krava obolelih od dislokacije sirišta na levo tri dana (P lt 0,05) i šest dana (P lt 0,01) nakon dijagnostikovanja bolesti. Tridesetog minuta nakon početka infuzije, hiperglikemija je utvrđena i kod zdravih i kod bolesnih krava. Iako je koncentracija glukoze i kod zdravih i kod bolesnih krava opadala od 60. do 180. minuta posle početka infuzije, hiperglikemija se kod svih krava održala tokom ovog perioda. U poređenju sa zdravim kravama, stepen opadanja koncentracije glukoze je bio sporiji kod bolesnih krava na dan dijagnostikovanja dislokacije sirišta na levo, kao i tri do šest dana posle. Na kraju eksperimenta, 240 minuta nakon početka infuzije, koncentracija glukoze je bila statistički značajno viša kod obolelih krava na dan dijagnostikovanja bolesti, kao i tri i šest dana kasnije u odnosu na vrednost određenu u to vreme kod kontrolne grupe krava (P lt 0,001, pojedinačno) i obolelih krava tri dana nakon repozicije abomasuma (P lt 0,001, pojedinačno). Bazalna vrednost insulinemije, određena neposredno pre početka infuzije glukoze, je bila značajno viša kod zdravih u odnosu na krave obolele od dislokacije sirišta šestog dana nakon postavljanja dijagnoze (P lt 0,001). U periodu od 30. do 240. minuta nakon početka infuzije glukoze, insulinski odgovor na aplikovanu glukozu je bio značajno viši kod zdravih krava u odnosu na bolesne krave na dan dijagnostikovanja dislokacije, tri i šest dana posle kao i 3 dana nakon hirurške repozicije abomasuma. Na dan dijagnostikovanja bolesti kao i tri dana kasnije, insulinemija je opala na vrednosti slične bazalnim 180. minuta nakon početka infuzije glukoze. Šest dana nakon dijagnostikovanja bolesti, koncentracija insulina je opala na vrednosti slične bazalnim 120 minuta nakon početka infuzije. Tri dana nakon repozicije abomasuma, insulinemija je smanjena na vrednosti slične bazalnim 240. minuta nakon početka infuzije. Rezultati testa opterećenja glukozom daju najbolju procenu endokrine funkcije pankreasa između 60. i 120. minuta i 120. i 180. minuta posle početka infuzije glukoze. Naši rezultati ukazuju da kod krava obolelih od dislokacije sirišta na levo postoji stanje insuficijencije endokrinog pankreasa i da se to stanje pogoršava sa dužim tokom bolesti. Najveći stepen insuficijencije je ustanovljen šesti dan od dijagnostikovanja oboljenja. Tri dana nakon izvođenja hirurške intervencije i repozicije dislociranog organa, rezultati testa opterećenja glukozom pokazuju da se ponovo uspostavlja normalna aktivnost endokrinog pankreasa.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Glucose tolerance test in the assessment of endocrine pancreatic function in cows before and after surgical correction of left diplaced abomasum
T1  - Primena testa opterećenja glukozom u oceni endokrine funkcije pankreasa kod krava obolelih od dislokacije sirišta na levo pre i posle hirurškog tretmana
VL  - 59
IS  - 5-6
SP  - 513
EP  - 523
DO  - 10.2298/AVB0906513S
ER  - 

@article{
author = "Šamanc, Horea and Stojić, Velibor and Kirovski, Danijela and Pudlo, Pavle and Vujanac, Ivan",
year = "2009",
abstract = "The aim of this study was to examine the secretory capacity of endocrine pancreas beta cells in healthy cows and cows suffering from left displaced abomasum (LDA) by determination of glucose and insulin concentrations in the peripheral circulation during glucose tolerance test (GTT). A total of twenty healthy cows (Control group) and twenty cows suffering from left abomasal displacement (Experimental group) were chosen for this study. Cows in the control group were exposed to GTT once, while cows in the experimental group were exposed on the day, day 3 and day 6 after the diagnosis of LDA, as well as 3 days after surgical treatment and reposition of the abomasum. Initial blood glucose concentrations in healthy cows were significantly lower than in the cows suffering from LDA only on day 3 (P lt 0.05) and day 6 (P lt 0.01) after the disease was diagnosed. All the glucose values determined both in healthy and diseased cows at 30. minutes after the start of glucose infusion were much higher than physiological values. Regardless to the decline of glucose levels from 60 to 180 minutes after start of glucose infusion, hyperglicemia maintained in both healthy cows and cows suffering from LDA. Compared to healthy cows the rate of decline was slower in cows suffering from LDA at the day of diagnosis, as well as 3 and 6 days after the diagnosis. At the end of the experiment, 240 minutes after the start of the infusion, glucose concentrations were significantly higher in diseased cows on the day of diagnosis and day 3 and 6 after diagnosis than in the control group (P lt 0.001, respectively) and in diseased cows 3 days after surgery (P lt 0.001, respectively). Mean initial concentrations of serum insulin were significantly higher in healthy cows than in cows suffering from LDA only on day 6 after diagnosis. From 30 to 240 minutes after the start of glucose infusion, insulinemic responses to intravenous glucose administation were statistically higher in healthy cows than in experimental cows on the day of diagnosis, day 3 and day 6 after diagnosis, as well as 3 days after surgery. On the day of diagnosis and three days after, insulinemia decreased to initial levels 180 minutes after the start of the infusion. Six days after diagnosis, insulinemia decreased to initial values at 120 minutes after the start of the glucose infusion. Three days after surgery, insulinemia decrased to values similar to initial at 240 minutes after the start of the infusion. Glucose tolerance test results showed that the best validation of pancreas endocrine function was between 60 and 120 minutes, as well as 180 and 240 minutes after the glucose infusion started. Our results also confirmed that cows suffering from LDA are in the stage of pancreatic endocrine disfunction and that the rate of this disfunction was higher when the disease lasted longer. The highest rate of pancreatic endocrine disfunction was observed 6 days after diagnosis. On day 3 after surgical treatment and reposition of the abomasum, glucose tolerance test results showed that the pancreatic endocrine function recovered., Cilj ovog rada bio je da se, određivanjem koncentracije glukoze i insulina u krvi krava tokom testa opterećenja glukozom, ispita sekretorni kapacitet beta ćelija endokrinog pankreasa kod zdravih krava i krava obolelih od dislokacije sirišta na levo. U ogled je uključeno 20 zdravih krava (kontrolna grupa) i 20 krava obolelih od dislokacije sirišta na levo (eksperimentalna grupa). Kod zdravih krava opterećenje glukozom je izvršeno jednom, dok je kod obolelih krava ovaj test izveden na dan dijagnostikovanja oboljenja, 3 i 6 dana posle, kao i 3 dana nakon hirurške intervencije i repozicije obolelog organa. Bazalna koncentracija glukoze, određena neposredno pre početka infuzije, je kod zdravih krava bila značajno niža nego kod krava obolelih od dislokacije sirišta na levo tri dana (P lt 0,05) i šest dana (P lt 0,01) nakon dijagnostikovanja bolesti. Tridesetog minuta nakon početka infuzije, hiperglikemija je utvrđena i kod zdravih i kod bolesnih krava. Iako je koncentracija glukoze i kod zdravih i kod bolesnih krava opadala od 60. do 180. minuta posle početka infuzije, hiperglikemija se kod svih krava održala tokom ovog perioda. U poređenju sa zdravim kravama, stepen opadanja koncentracije glukoze je bio sporiji kod bolesnih krava na dan dijagnostikovanja dislokacije sirišta na levo, kao i tri do šest dana posle. Na kraju eksperimenta, 240 minuta nakon početka infuzije, koncentracija glukoze je bila statistički značajno viša kod obolelih krava na dan dijagnostikovanja bolesti, kao i tri i šest dana kasnije u odnosu na vrednost određenu u to vreme kod kontrolne grupe krava (P lt 0,001, pojedinačno) i obolelih krava tri dana nakon repozicije abomasuma (P lt 0,001, pojedinačno). Bazalna vrednost insulinemije, određena neposredno pre početka infuzije glukoze, je bila značajno viša kod zdravih u odnosu na krave obolele od dislokacije sirišta šestog dana nakon postavljanja dijagnoze (P lt 0,001). U periodu od 30. do 240. minuta nakon početka infuzije glukoze, insulinski odgovor na aplikovanu glukozu je bio značajno viši kod zdravih krava u odnosu na bolesne krave na dan dijagnostikovanja dislokacije, tri i šest dana posle kao i 3 dana nakon hirurške repozicije abomasuma. Na dan dijagnostikovanja bolesti kao i tri dana kasnije, insulinemija je opala na vrednosti slične bazalnim 180. minuta nakon početka infuzije glukoze. Šest dana nakon dijagnostikovanja bolesti, koncentracija insulina je opala na vrednosti slične bazalnim 120 minuta nakon početka infuzije. Tri dana nakon repozicije abomasuma, insulinemija je smanjena na vrednosti slične bazalnim 240. minuta nakon početka infuzije. Rezultati testa opterećenja glukozom daju najbolju procenu endokrine funkcije pankreasa između 60. i 120. minuta i 120. i 180. minuta posle početka infuzije glukoze. Naši rezultati ukazuju da kod krava obolelih od dislokacije sirišta na levo postoji stanje insuficijencije endokrinog pankreasa i da se to stanje pogoršava sa dužim tokom bolesti. Najveći stepen insuficijencije je ustanovljen šesti dan od dijagnostikovanja oboljenja. Tri dana nakon izvođenja hirurške intervencije i repozicije dislociranog organa, rezultati testa opterećenja glukozom pokazuju da se ponovo uspostavlja normalna aktivnost endokrinog pankreasa.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Glucose tolerance test in the assessment of endocrine pancreatic function in cows before and after surgical correction of left diplaced abomasum, Primena testa opterećenja glukozom u oceni endokrine funkcije pankreasa kod krava obolelih od dislokacije sirišta na levo pre i posle hirurškog tretmana",
volume = "59",
number = "5-6",
pages = "513-523",
doi = "10.2298/AVB0906513S"
}

Šamanc, H., Stojić, V., Kirovski, D., Pudlo, P.,& Vujanac, I.. (2009). Glucose tolerance test in the assessment of endocrine pancreatic function in cows before and after surgical correction of left diplaced abomasum. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 59(5-6), 513-523.
https://doi.org/10.2298/AVB0906513S

Šamanc H, Stojić V, Kirovski D, Pudlo P, Vujanac I. Glucose tolerance test in the assessment of endocrine pancreatic function in cows before and after surgical correction of left diplaced abomasum. in Acta Veterinaria-Beograd. 2009;59(5-6):513-523.
doi:10.2298/AVB0906513S .

Šamanc, Horea, Stojić, Velibor, Kirovski, Danijela, Pudlo, Pavle, Vujanac, Ivan, "Glucose tolerance test in the assessment of endocrine pancreatic function in cows before and after surgical correction of left diplaced abomasum" in Acta Veterinaria-Beograd, 59, no. 5-6 (2009):513-523,
https://doi.org/10.2298/AVB0906513S . .

TY  - JOUR
AU  - Gvozdić, Dragan
AU  - Stojić, Velibor
AU  - Šamanc, Horea
AU  - Fratrić, Natalija
AU  - Daković, Aleksandra
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/563
AB  - Sixty Holstein newborn calves, which were immediately after parturition separated from their dams and placed in individual pens, were randomly assigned to 4 treatment groups (15 calves per group). All calves were bottle-fed twice a day during the first 48 hours after partus, with their mothers' colostrum in 12 hour intervals, starting two hours after partus, according to the following model: i) group 1 (G1) received 0.75 L of colostrum (C) per meal, with 20 mL of 25% zeolite (clinoptilolite) suspension in distilled water; ii) group 2 (G2) received 1.5L of C per meal, with 20 mL of 25% zeolite suspension in distilled water; iii) group 3 (G3) received 0.75 L of C per meal, and iv) group 4 (G4) received 1.5 L of C per meal. Blood serum immunoglobulin G (IgG) concentrations at 0h (before C ingestion) 6h, 24h and 48h after partus and colostrum IgG concentrations were determined using commercial sRID plates (INEP, Zemun, Serbia). Apparent efficiency of absorption (AEA%) was calculated for individual calves at 6h after partus. Calf blood serum IgG concentrations at all investigated time intervals in G1 were increased compared to G3, with a significant increase at 6h after partus (15±4:10±5 g/L, p lt 0.05). The same relationship was evident between G2 and G4, with significant mean IgG blood serum differences evident at 6, 24. and 48. hour after partus. Calf blood serum IgG concentration was significantly higher in G2 compared to all experimental groups at 6, 24 and 48h after partus. Apparent efficiency of absorption (AEA%) at 6h after partus was significantly higher in G1, compared to all other experimental groups.
AB  - Šezdeset novorođenih teladi Holštajn rase, koja su neposredno nakon rođenja odvojena od majki i stavljena u individualne bokseve, su metodom slučajnog izbora podeljene u 4 ogledne grupe (15 teladi po grupi). Sva telad su za vreme prvih 48 sati života hranjena dva puta dnevno (u intervalu od 12 sati) kolostrumom majki, počevši od drugog sata nakon partusa prema sledećem eksperimentalnom modelu: i) grupa 1 (G1) dobijala je 0.75 L kolostruma (C) po obroku, sa dodatkom 20 mL suspenzije 25% zeolita (klinoptilolita) u destilovanoj vodi; ii) grupa 2 (G2) dobijala je 1.5 L C po obroku, sa dodatkom 20 mL suspenzije 25% zeolita (klinoptilolita) u destilovanoj vodi; iii) grupa 3 (G3) dobijala je 0.75L C po obroku; iv) grupa 4 (G4) dobijala je 1.5 L C po obroku. Koncentracija imunoglobulina G (IgG) određivana je komercijalnim radijalnim imunodifuzionim testom (sRID, INEP, Zemin) u krvnom serumu dobijenom iz uzoraka krvi uzetih punkcijom v. jugularis pre uzimanja kolosruma (0. sat), 6-og, 24-og i 48-og sata nakon rođenja, dok je relativna efikasnost apsorpcije imunoglobulina (AEA%) određena 6-og sata po rođenju. Koncentracija IgG u krvnom serumu kod teladi iz grupe G1 u odnosu na G2 bila je viša 6-og, 24-og i 48-og sata, dok je statistička značajnost razlika srednjih vrednosti ustanovljena 6-og sata po rođenju (15±4:10±5 g/L, p lt 0.05). Isti odnos srednjih vrednosti koncentracije IgG u krvnom serumu novorođenih teladi je zabeležen između grupe G2 i G4, gde su statistički značajno više srednje vrednosti utvrđene kod grupe G2 u odnosu na G4 u periodu 6, 24. i 48. sati po rođenju (p lt 0.01 za sve navedene vremenske periode). Relativna efikasnost apsorpcije 6-og sata po rođenju je bila statistički vrlo značajno viša (p lt 0.01) kod grupe G1 u odnosu na ostale ogledne grupe teladi.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Apparent efficiency of immunoglobulin absorption in newborn calves orally treated with zeolite
T1  - Relativna efikasnost resorpcije imunoglobulina kod novorođene teladi oralno tretirane zeolitom
VL  - 58
IS  - 4
SP  - 345
EP  - 355
DO  - 10.2298/AVB0804345G
ER  - 

@article{
author = "Gvozdić, Dragan and Stojić, Velibor and Šamanc, Horea and Fratrić, Natalija and Daković, Aleksandra",
year = "2008",
abstract = "Sixty Holstein newborn calves, which were immediately after parturition separated from their dams and placed in individual pens, were randomly assigned to 4 treatment groups (15 calves per group). All calves were bottle-fed twice a day during the first 48 hours after partus, with their mothers' colostrum in 12 hour intervals, starting two hours after partus, according to the following model: i) group 1 (G1) received 0.75 L of colostrum (C) per meal, with 20 mL of 25% zeolite (clinoptilolite) suspension in distilled water; ii) group 2 (G2) received 1.5L of C per meal, with 20 mL of 25% zeolite suspension in distilled water; iii) group 3 (G3) received 0.75 L of C per meal, and iv) group 4 (G4) received 1.5 L of C per meal. Blood serum immunoglobulin G (IgG) concentrations at 0h (before C ingestion) 6h, 24h and 48h after partus and colostrum IgG concentrations were determined using commercial sRID plates (INEP, Zemun, Serbia). Apparent efficiency of absorption (AEA%) was calculated for individual calves at 6h after partus. Calf blood serum IgG concentrations at all investigated time intervals in G1 were increased compared to G3, with a significant increase at 6h after partus (15±4:10±5 g/L, p lt 0.05). The same relationship was evident between G2 and G4, with significant mean IgG blood serum differences evident at 6, 24. and 48. hour after partus. Calf blood serum IgG concentration was significantly higher in G2 compared to all experimental groups at 6, 24 and 48h after partus. Apparent efficiency of absorption (AEA%) at 6h after partus was significantly higher in G1, compared to all other experimental groups., Šezdeset novorođenih teladi Holštajn rase, koja su neposredno nakon rođenja odvojena od majki i stavljena u individualne bokseve, su metodom slučajnog izbora podeljene u 4 ogledne grupe (15 teladi po grupi). Sva telad su za vreme prvih 48 sati života hranjena dva puta dnevno (u intervalu od 12 sati) kolostrumom majki, počevši od drugog sata nakon partusa prema sledećem eksperimentalnom modelu: i) grupa 1 (G1) dobijala je 0.75 L kolostruma (C) po obroku, sa dodatkom 20 mL suspenzije 25% zeolita (klinoptilolita) u destilovanoj vodi; ii) grupa 2 (G2) dobijala je 1.5 L C po obroku, sa dodatkom 20 mL suspenzije 25% zeolita (klinoptilolita) u destilovanoj vodi; iii) grupa 3 (G3) dobijala je 0.75L C po obroku; iv) grupa 4 (G4) dobijala je 1.5 L C po obroku. Koncentracija imunoglobulina G (IgG) određivana je komercijalnim radijalnim imunodifuzionim testom (sRID, INEP, Zemin) u krvnom serumu dobijenom iz uzoraka krvi uzetih punkcijom v. jugularis pre uzimanja kolosruma (0. sat), 6-og, 24-og i 48-og sata nakon rođenja, dok je relativna efikasnost apsorpcije imunoglobulina (AEA%) određena 6-og sata po rođenju. Koncentracija IgG u krvnom serumu kod teladi iz grupe G1 u odnosu na G2 bila je viša 6-og, 24-og i 48-og sata, dok je statistička značajnost razlika srednjih vrednosti ustanovljena 6-og sata po rođenju (15±4:10±5 g/L, p lt 0.05). Isti odnos srednjih vrednosti koncentracije IgG u krvnom serumu novorođenih teladi je zabeležen između grupe G2 i G4, gde su statistički značajno više srednje vrednosti utvrđene kod grupe G2 u odnosu na G4 u periodu 6, 24. i 48. sati po rođenju (p lt 0.01 za sve navedene vremenske periode). Relativna efikasnost apsorpcije 6-og sata po rođenju je bila statistički vrlo značajno viša (p lt 0.01) kod grupe G1 u odnosu na ostale ogledne grupe teladi.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Apparent efficiency of immunoglobulin absorption in newborn calves orally treated with zeolite, Relativna efikasnost resorpcije imunoglobulina kod novorođene teladi oralno tretirane zeolitom",
volume = "58",
number = "4",
pages = "345-355",
doi = "10.2298/AVB0804345G"
}

Gvozdić, D., Stojić, V., Šamanc, H., Fratrić, N.,& Daković, A.. (2008). Apparent efficiency of immunoglobulin absorption in newborn calves orally treated with zeolite. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(4), 345-355.
https://doi.org/10.2298/AVB0804345G

Gvozdić D, Stojić V, Šamanc H, Fratrić N, Daković A. Apparent efficiency of immunoglobulin absorption in newborn calves orally treated with zeolite. in Acta Veterinaria-Beograd. 2008;58(4):345-355.
doi:10.2298/AVB0804345G .

Gvozdić, Dragan, Stojić, Velibor, Šamanc, Horea, Fratrić, Natalija, Daković, Aleksandra, "Apparent efficiency of immunoglobulin absorption in newborn calves orally treated with zeolite" in Acta Veterinaria-Beograd, 58, no. 4 (2008):345-355,
https://doi.org/10.2298/AVB0804345G . .

TY  - JOUR
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Mirilović, Milorad
AU  - Stojić, Velibor
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/551
AB  - Breeding grey A. m. carnica queens for hygienic behavior is beneficial for commercial beekeeping in Serbia, since hygienic behavior is associated with the resistance to the mite Varroa destructor, as well as to American foulbrood and chalk brood. In this work, heritability of hygienic behavior was analyzed in autochthonous grey A. m. carnica honey bees from Šumadija region in order to ascertain if the expression of their hygienic behavior could be enhanced through selective breeding. Heritability of hygienic behavior was monitored through three queen generations and estimated by one-parent-offspring regression method (motherdaughter regression method). The heritability values for hygienic behavior were h2=0.63±0.02 in relationship between daughter queens (F1s) and breeder mother queens (Ps), h2=0.45±0.01 in relationship between grand-daughter queens (F2s) and Ps and h2=0.44±0.02 in relationship between F2s and F1s. These results show that the expression of hygienic behavior through selective breeding could be enhanced and the best results could be achieved in the F1 generation. It is important to emphasize that the F1 colonies bred for hygienic behavior in our study suffer no apparent costs in reproductive and productive performances.
AB  - Odgajanje matica sive medonosne pčele A. m. carnica sa ispoljenim higijenskim ponašanjem od velikog je značaja za komercijalno pčelarenje u Srbiji, obzirom da je higijensko ponašanje povezano sa otpornošću prema krpelju Varroa destructor, kao i prema izazivačima bolesti američke truleži pčelinjeg legla i krečnog legla. U ovom radu, ispitivana je heritabilnost higijenskog ponašanja kod autohtone sive medonosne pčele A. m. carnica iz Šumadije da bi se utvrdilo da li ispoljenost higijenskog ponašanja kod tih pčela može da se poveća putem selektivnog gajenja. Heritabilnost higijenskog ponašanja praćena je kroz tri generacije matica i procenjivana metodom regresije jedan roditelj-potomak (metoda regresije majka-ćerka). Vrednosti koeficijenta heritabilnosti higijenskog ponašanja bile su h2=0,63±0,02 između ćerki matica (F1s) i majki matica (Ps), h2=0,45±0,01 između unuka matica (F2s) i Ps, i h2=0.44±0.02 između F2s i F1s. Ovi rezultati pokazuju da ispoljenost higijenskog ponašanja kod analiziranih pčela može biti povećana, kao i da se najbolji rezultati mogu postići u F1 generaciji. Pri tome je značajno istaći da kod društava F1 generacije odgajenih na higijensko ponašanje u našem radu nije bilo nikakvih negativnih posledica u pogledu reproduktivnih i produktivnih sposobnosti.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Heritability of hygienic behavior in grey honey bees (Apis mellifera carnica)
T1  - Heritabilnost higijenskog ponašanja sive medonosne pčele (Apis mellifera carnica)
VL  - 58
IS  - 5-6
SP  - 593
EP  - 601
DO  - 10.2298/AVB0806593S
ER  - 

@article{
author = "Stanimirović, Zoran and Stevanović, Jevrosima and Mirilović, Milorad and Stojić, Velibor",
year = "2008",
abstract = "Breeding grey A. m. carnica queens for hygienic behavior is beneficial for commercial beekeeping in Serbia, since hygienic behavior is associated with the resistance to the mite Varroa destructor, as well as to American foulbrood and chalk brood. In this work, heritability of hygienic behavior was analyzed in autochthonous grey A. m. carnica honey bees from Šumadija region in order to ascertain if the expression of their hygienic behavior could be enhanced through selective breeding. Heritability of hygienic behavior was monitored through three queen generations and estimated by one-parent-offspring regression method (motherdaughter regression method). The heritability values for hygienic behavior were h2=0.63±0.02 in relationship between daughter queens (F1s) and breeder mother queens (Ps), h2=0.45±0.01 in relationship between grand-daughter queens (F2s) and Ps and h2=0.44±0.02 in relationship between F2s and F1s. These results show that the expression of hygienic behavior through selective breeding could be enhanced and the best results could be achieved in the F1 generation. It is important to emphasize that the F1 colonies bred for hygienic behavior in our study suffer no apparent costs in reproductive and productive performances., Odgajanje matica sive medonosne pčele A. m. carnica sa ispoljenim higijenskim ponašanjem od velikog je značaja za komercijalno pčelarenje u Srbiji, obzirom da je higijensko ponašanje povezano sa otpornošću prema krpelju Varroa destructor, kao i prema izazivačima bolesti američke truleži pčelinjeg legla i krečnog legla. U ovom radu, ispitivana je heritabilnost higijenskog ponašanja kod autohtone sive medonosne pčele A. m. carnica iz Šumadije da bi se utvrdilo da li ispoljenost higijenskog ponašanja kod tih pčela može da se poveća putem selektivnog gajenja. Heritabilnost higijenskog ponašanja praćena je kroz tri generacije matica i procenjivana metodom regresije jedan roditelj-potomak (metoda regresije majka-ćerka). Vrednosti koeficijenta heritabilnosti higijenskog ponašanja bile su h2=0,63±0,02 između ćerki matica (F1s) i majki matica (Ps), h2=0,45±0,01 između unuka matica (F2s) i Ps, i h2=0.44±0.02 između F2s i F1s. Ovi rezultati pokazuju da ispoljenost higijenskog ponašanja kod analiziranih pčela može biti povećana, kao i da se najbolji rezultati mogu postići u F1 generaciji. Pri tome je značajno istaći da kod društava F1 generacije odgajenih na higijensko ponašanje u našem radu nije bilo nikakvih negativnih posledica u pogledu reproduktivnih i produktivnih sposobnosti.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Heritability of hygienic behavior in grey honey bees (Apis mellifera carnica), Heritabilnost higijenskog ponašanja sive medonosne pčele (Apis mellifera carnica)",
volume = "58",
number = "5-6",
pages = "593-601",
doi = "10.2298/AVB0806593S"
}

Stanimirović, Z., Stevanović, J., Mirilović, M.,& Stojić, V.. (2008). Heritability of hygienic behavior in grey honey bees (Apis mellifera carnica). in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 58(5-6), 593-601.
https://doi.org/10.2298/AVB0806593S

Stanimirović Z, Stevanović J, Mirilović M, Stojić V. Heritability of hygienic behavior in grey honey bees (Apis mellifera carnica). in Acta Veterinaria-Beograd. 2008;58(5-6):593-601.
doi:10.2298/AVB0806593S .

Stanimirović, Zoran, Stevanović, Jevrosima, Mirilović, Milorad, Stojić, Velibor, "Heritability of hygienic behavior in grey honey bees (Apis mellifera carnica)" in Acta Veterinaria-Beograd, 58, no. 5-6 (2008):593-601,
https://doi.org/10.2298/AVB0806593S . .

TY  - JOUR
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Radaković, Milena
AU  - Stojić, Velibor
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/545
AB  - Fumogillin, an antibiotic compound produced by Aspergillus fumigatus, is effective against microsporidia and various Amoeba species, but is also toxic when administered systemically to mammals. Furthermore, a recent in vivo study by Stanimirovic Z et al. [2007]: (Mutat Res 628: 1-10) indicated genotoxic effects of fumagillin. The aim of the present study was to investigate and explain the clostogenic effects of fumagillin (in the form of fumagillin dicyclohexylamine salt) on human peripheral blood lymphocytes in vitro by sister-chromatid exchanges (SCE), chromosome aberrations (CA), and micronucleus (MN) tests. The mitotic index (MI), proliferation index (PI), and nuclear division index (NDI) were calculated to evaluate the cytotoxic potential of fumagillin. Five concentrations of fumagillin (0.34, 0.68, 1.02, 3.07, and 9.20 mu g/ml) were applied to lymphocyte cultures. All the tested concentrations of fumagillin increased the frequency of SCE per cell significantly (P < 0.001 or P < 0.01) compared with the negative control. A significant (P < 0.001) increase in frequency of structural CA was observed at the three highest concentrations in comparison with the negative control. In addition, the three highest test concentrations increased MN formation and decreased MI, PI, and NDI significantly compared with the negative control. The present results indicate that fumagillin is clostogenic and cytotoxic to cultured human lymphocytes. Environ. Mol. Mutagen. 49:594-601, 2008.
PB  - Wiley, Hoboken
T2  - Environmental and Molecular Mutagenesis
T1  - In Vitro Evaluation of the Clastogenicity of Fumagillin
VL  - 49
IS  - 8
SP  - 594
EP  - 601
DO  - 10.1002/em.20409
ER  - 

@article{
author = "Stevanović, Jevrosima and Stanimirović, Zoran and Radaković, Milena and Stojić, Velibor",
year = "2008",
abstract = "Fumogillin, an antibiotic compound produced by Aspergillus fumigatus, is effective against microsporidia and various Amoeba species, but is also toxic when administered systemically to mammals. Furthermore, a recent in vivo study by Stanimirovic Z et al. [2007]: (Mutat Res 628: 1-10) indicated genotoxic effects of fumagillin. The aim of the present study was to investigate and explain the clostogenic effects of fumagillin (in the form of fumagillin dicyclohexylamine salt) on human peripheral blood lymphocytes in vitro by sister-chromatid exchanges (SCE), chromosome aberrations (CA), and micronucleus (MN) tests. The mitotic index (MI), proliferation index (PI), and nuclear division index (NDI) were calculated to evaluate the cytotoxic potential of fumagillin. Five concentrations of fumagillin (0.34, 0.68, 1.02, 3.07, and 9.20 mu g/ml) were applied to lymphocyte cultures. All the tested concentrations of fumagillin increased the frequency of SCE per cell significantly (P < 0.001 or P < 0.01) compared with the negative control. A significant (P < 0.001) increase in frequency of structural CA was observed at the three highest concentrations in comparison with the negative control. In addition, the three highest test concentrations increased MN formation and decreased MI, PI, and NDI significantly compared with the negative control. The present results indicate that fumagillin is clostogenic and cytotoxic to cultured human lymphocytes. Environ. Mol. Mutagen. 49:594-601, 2008.",
publisher = "Wiley, Hoboken",
journal = "Environmental and Molecular Mutagenesis",
title = "In Vitro Evaluation of the Clastogenicity of Fumagillin",
volume = "49",
number = "8",
pages = "594-601",
doi = "10.1002/em.20409"
}

Stevanović, J., Stanimirović, Z., Radaković, M.,& Stojić, V.. (2008). In Vitro Evaluation of the Clastogenicity of Fumagillin. in Environmental and Molecular Mutagenesis
Wiley, Hoboken., 49(8), 594-601.
https://doi.org/10.1002/em.20409

Stevanović J, Stanimirović Z, Radaković M, Stojić V. In Vitro Evaluation of the Clastogenicity of Fumagillin. in Environmental and Molecular Mutagenesis. 2008;49(8):594-601.
doi:10.1002/em.20409 .

Stevanović, Jevrosima, Stanimirović, Zoran, Radaković, Milena, Stojić, Velibor, "In Vitro Evaluation of the Clastogenicity of Fumagillin" in Environmental and Molecular Mutagenesis, 49, no. 8 (2008):594-601,
https://doi.org/10.1002/em.20409 . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Stojić, Velibor
AU  - Kirovski, Danijela
AU  - Jovanović, Milijan
AU  - Cernescu, Horia
AU  - Vujanac, Ivan
AU  - Prodanović, Radiša
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/512
AB  - The aim of this study was to investigate the influence of energy status of cows, estimated by body condition score, on the incidence and the degree of hepatic lipidosis during the early stage of lactation. Cows were divided into four groups: dry, early puerperal, early lactation and late lactation period. Each group consisted of 60 cows. Body condition was scored by the method established in Elaco Animal Health Buletin Al 8478. According to this method, body condition score is represented numerically from 1 to 5 points. Liver samples for pathohistological analyses were taken by biopsy from early lactation cows (60. day of lactation). Fat content in hepatocytes was determined morphometrically and results were compared with criteria based on total lipid and triglyceride content in liver tissue. On farm A, average body condition scores were in a range from 3.31, at sixty days of lactation, to 3.86 points in the dry period. Nevertheless, the range was wider on farm B, and was from 2.18 points at sixty days of lactation to 4.15 points at the dry period. Besides, differences in average body condition scores were higher than 1 point, and in some cases (between late lactation or dry period to sixty days of lactation) were almost 2 points. The incidence and the degree of hepatic lipidosis strongly differ between the two examined farms. On farm A the incidence of hepatic lipidosis was 18.33 percent, while on farm B that percent was much higher (43.32). A significant difference was established in the degree of hepatic lipidosis between those two farms. On farm A diffuse hepatic lipidosis was determined in 5.0 percent of cows, while on farm B that percent was 18.33. According to these results, most of the cows on farm B had uncontrolled lipomobilisation and severe fatty liver during the early lactation period, probably due to the obesity of these cows in late lactation and the dry period.
AB  - Cilj istraživanja, obrađenog u ovom radu, bio je da se utvrdi uticaj energetskog statusa krava, procenjen na osnovu telesne kondicije, na učestalost i stepen zamašćenja jetre u ranoj fazi laktacije. Za ispitivanja su odabrane 4 grupe od po 60 krava koje su bile u periodu zasušenja, ranom puerperijumu, ranoj i kasnoj fazi laktacije. Telesna kondicija krava uključenih u ogled je ocenjivana prema sistemu Elanco Animal Health Buletin Al 8478. Prema ovom sistemu ocena telesne kondicije se izražava numerički od 1 do 5 poena. Od krava koje su bile u ranoj fazi laktacije (60. dana) biopsijom su uzimani uzorci tkiva jetre za patohistološ ka ispitivanja. Sadržaj masti u hepatocitima određivan je morfometrijski a dobijeni rezultati su upoređivani sa kriterijumima dobijenim na osnovu količine ukupnih lipida i triglicerida u tkivu jetre. Na farmi A prosečne vrednosti ocena telesne kondicije su se kretale od najmanje 3,31 60. dana laktacije, do najviše 3,86 poena u zasušenju. Na farmi B su utvrđena veća odstupanja, tako da je prosečna vrednost ocene telesne kondicije 60. dana laktacije bila 2,18 poena, a u zasušenju 4,15 poena. Osim toga, na farmi B razlike između prosečnih vrednosti ocena telesne kondicije su veće od 1 poena, a u nekim slučajevima (između poslednje faze laktacije i perioda zasušenja u odnosu na šezdeseti dan laktacije) su skoro 2 poena. Rezultati ispitivanja učestalosti i stepena zamašćenja jetre dobijeni na dve farme se takođe značajno razlikuju. Dok je na farmi A zamašćenje jetre ustanovljeno kod 18,33% krava, na farmi B je taj procenat bio značajno veći (43,32). Značajne razlike postoje i u stepenu zamašćenja jetre krava sa ove dve farme. Na farmi A difuzno zamašćenje jetre je ustanovljeno kod 5%, a na farmi B kod 18,33% ispitivanih životinja. Po svemu sudeći, na farmi B, kao rezultat gojaznosti većine krava u poslednjoj fazi laktacije i zasušenju, u ranoj fazi laktacije započinje nekontrolisana lipomobilizacija i nastaje težak oblik zamašćenja jetre.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Influence of body condition on incidence and degree of hepatic lipidosis in cows
T1  - Uticaj telesne kondicije krava na učestalost i stepen zamašćenja jetre
VL  - 62
IS  - 1-2
SP  - 3
EP  - 12
DO  - 10.2298/VETGL0802003S
ER  - 

@article{
author = "Šamanc, Horea and Stojić, Velibor and Kirovski, Danijela and Jovanović, Milijan and Cernescu, Horia and Vujanac, Ivan and Prodanović, Radiša",
year = "2008",
abstract = "The aim of this study was to investigate the influence of energy status of cows, estimated by body condition score, on the incidence and the degree of hepatic lipidosis during the early stage of lactation. Cows were divided into four groups: dry, early puerperal, early lactation and late lactation period. Each group consisted of 60 cows. Body condition was scored by the method established in Elaco Animal Health Buletin Al 8478. According to this method, body condition score is represented numerically from 1 to 5 points. Liver samples for pathohistological analyses were taken by biopsy from early lactation cows (60. day of lactation). Fat content in hepatocytes was determined morphometrically and results were compared with criteria based on total lipid and triglyceride content in liver tissue. On farm A, average body condition scores were in a range from 3.31, at sixty days of lactation, to 3.86 points in the dry period. Nevertheless, the range was wider on farm B, and was from 2.18 points at sixty days of lactation to 4.15 points at the dry period. Besides, differences in average body condition scores were higher than 1 point, and in some cases (between late lactation or dry period to sixty days of lactation) were almost 2 points. The incidence and the degree of hepatic lipidosis strongly differ between the two examined farms. On farm A the incidence of hepatic lipidosis was 18.33 percent, while on farm B that percent was much higher (43.32). A significant difference was established in the degree of hepatic lipidosis between those two farms. On farm A diffuse hepatic lipidosis was determined in 5.0 percent of cows, while on farm B that percent was 18.33. According to these results, most of the cows on farm B had uncontrolled lipomobilisation and severe fatty liver during the early lactation period, probably due to the obesity of these cows in late lactation and the dry period., Cilj istraživanja, obrađenog u ovom radu, bio je da se utvrdi uticaj energetskog statusa krava, procenjen na osnovu telesne kondicije, na učestalost i stepen zamašćenja jetre u ranoj fazi laktacije. Za ispitivanja su odabrane 4 grupe od po 60 krava koje su bile u periodu zasušenja, ranom puerperijumu, ranoj i kasnoj fazi laktacije. Telesna kondicija krava uključenih u ogled je ocenjivana prema sistemu Elanco Animal Health Buletin Al 8478. Prema ovom sistemu ocena telesne kondicije se izražava numerički od 1 do 5 poena. Od krava koje su bile u ranoj fazi laktacije (60. dana) biopsijom su uzimani uzorci tkiva jetre za patohistološ ka ispitivanja. Sadržaj masti u hepatocitima određivan je morfometrijski a dobijeni rezultati su upoređivani sa kriterijumima dobijenim na osnovu količine ukupnih lipida i triglicerida u tkivu jetre. Na farmi A prosečne vrednosti ocena telesne kondicije su se kretale od najmanje 3,31 60. dana laktacije, do najviše 3,86 poena u zasušenju. Na farmi B su utvrđena veća odstupanja, tako da je prosečna vrednost ocene telesne kondicije 60. dana laktacije bila 2,18 poena, a u zasušenju 4,15 poena. Osim toga, na farmi B razlike između prosečnih vrednosti ocena telesne kondicije su veće od 1 poena, a u nekim slučajevima (između poslednje faze laktacije i perioda zasušenja u odnosu na šezdeseti dan laktacije) su skoro 2 poena. Rezultati ispitivanja učestalosti i stepena zamašćenja jetre dobijeni na dve farme se takođe značajno razlikuju. Dok je na farmi A zamašćenje jetre ustanovljeno kod 18,33% krava, na farmi B je taj procenat bio značajno veći (43,32). Značajne razlike postoje i u stepenu zamašćenja jetre krava sa ove dve farme. Na farmi A difuzno zamašćenje jetre je ustanovljeno kod 5%, a na farmi B kod 18,33% ispitivanih životinja. Po svemu sudeći, na farmi B, kao rezultat gojaznosti većine krava u poslednjoj fazi laktacije i zasušenju, u ranoj fazi laktacije započinje nekontrolisana lipomobilizacija i nastaje težak oblik zamašćenja jetre.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Influence of body condition on incidence and degree of hepatic lipidosis in cows, Uticaj telesne kondicije krava na učestalost i stepen zamašćenja jetre",
volume = "62",
number = "1-2",
pages = "3-12",
doi = "10.2298/VETGL0802003S"
}

Šamanc, H., Stojić, V., Kirovski, D., Jovanović, M., Cernescu, H., Vujanac, I.,& Prodanović, R.. (2008). Influence of body condition on incidence and degree of hepatic lipidosis in cows. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(1-2), 3-12.
https://doi.org/10.2298/VETGL0802003S

Šamanc H, Stojić V, Kirovski D, Jovanović M, Cernescu H, Vujanac I, Prodanović R. Influence of body condition on incidence and degree of hepatic lipidosis in cows. in Veterinarski Glasnik. 2008;62(1-2):3-12.
doi:10.2298/VETGL0802003S .

Šamanc, Horea, Stojić, Velibor, Kirovski, Danijela, Jovanović, Milijan, Cernescu, Horia, Vujanac, Ivan, Prodanović, Radiša, "Influence of body condition on incidence and degree of hepatic lipidosis in cows" in Veterinarski Glasnik, 62, no. 1-2 (2008):3-12,
https://doi.org/10.2298/VETGL0802003S . .

TY  - JOUR
AU  - Fratrić, Natalija
AU  - Stojić, Velibor
AU  - Rajčić, S.
AU  - Radojičić, Biljana
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/472
AB  - The aim of the study was to investigate the influence of added natural mineral adsorbent zeolite (clinoptylolite) on the degree of absorption of immunoglobulin G and on glucose and protein concentration in the blood of calves during the neonatal period. Sixty calves were randomly divided in to four groups (15 calves per group). The quality and timing of colostrum feeding was as follows: group I was fed only with 1.5 L of colostrum at 2nd, 12th, 24th and 36th hour after birth; group II was fed with 1.5 L of colostrum with the mineral adsorbent added at a 5% concentration in the same time intervals; group III was fed with 1.5 L of colostrum together with the mineral adsorbent added at a 5% concentration, but at 2nd and 12 hour after birth it was fed with the first colostrum, and 24 and 36 hours after birth it was fed with the second colostrum. Group IV of calves was fed the same way as the third one only without the addition of the mineral adsorbent. Mean serum concentration of IgG was 15.14±7.43; 22.22±8.15; 23.65±9.93; 20.81±4.19 g/L in groups I to IV respectively at 6h of age. IgG concentration in the sera of all groups were 29.10±15.25; 45.46±13.45; 50.72±21.57; 41.60±15.42 g/L respectively at 16h after birth. At 30 hours of age, concentration of IgG was 33.29±14.70; 47.05±12.38; 55.00±19.71; 51.59±13.82 g/L in groups I to IV respectively. IgG concentration in the sera of all groups were 29.82±12.69; 44.10±13.50; 51.88±20.14; 47.68±13.18 g/L respectively at 40 hours after birth. For the calves which were fed with the full colostrum without and with the addition of the mineral adsorbent (Group I and II) the statistical significance of the difference in the concentration of IgG was determined during all the tested intervals. Concentration of IgG in the sera of group II calves (fed colostrum with zeolite added) was approximately 30% higher than in the group I. The statistical significance of the difference in levels of IgG between group I and groups III an IV was determined during all tested intervals, also. Concentration of IgG in the sera of group III calves was 40% higher than in group I. Acta Veterinaria (Beograd), Vol. 57, No. 2-3, 169-180, 2007. Results suggest that calves should receive feedings of high quality colostrum with added mineral adsorbent in order to maximize colostral IgG absorption. Determining the proteinemia in the neonatal period of calves is a reliable indicator of the impact of the mineral adsorbent on the degree of absorption of colostral IgG. The concentration of glucose for all the tested groups increased significantly with the increase of the quantity of ingested colostrum, with no evident influence of the added mineral adsorbent.
AB  - Cilj ovog rada bio je da se ispita uticaj mineralnog adsorbenta na bazi klinoptilolita na stepen resorpcije IgG i koncentraciju nekih biohemijskih parametara u krvnom serumu teladi u neonatalnom periodu. Telad su bila podeljena u četiri grupe (po 15 teladi u svakoj grupi). I grupa teladi napajana je sa kolostrumom 2h, 12h, 24h i 36h posle rođenja, II grupa teladi napajana je sa kolostrumom u koji je dodat mineralni adsorbent u koncentraciji od 5% u istim vremenskim intervalima, III grupa napajana je sa 1,5 L kolostrumom u koji je dodat mineralni adsorbent u koncentraciji od 5% ali 2h i 12h posle rođenja napajana su prvim kolostrumom, a 24h i 36h dugim kolostrumom, IV grupa teladi hranjena je na isti način kao i III grupa samo što u kolostrum nije bio dodat mineralni adsorbent. Koncentracija IgG u krvnom serumu teladi od I do IV grupe, 6 sata iznosila je pojedinačno 15,14±7,43; 22,22±8,15; 23,65±9,93; 20,81±4,19 g/L. U krvnom serumu teladi kod svih ispitivanih grupa koncentracija IgG u 16h iznosila je pojedinač no 29,10±15,25; 45,46±13,45; 50,72±21,57; 41,60±15,42 g/L. Koncentracija IgG 30sata kod ispitivanih grupa teladi (I do IV) bila je pojedinačno 33,29±14,70; 47,05±12,38; 55,00±19,71; 51,59±13,82 g/L. Koncentracija IgG u krvnom serumu svih grupa 40h iznosila je 29,82±12,69; 44,10±13,50; 51,88± 20,14; 47,68±13,18 g/L pojedinačno. Kod teladi koja su napajana punim kolostrumom bez i sa dodatkom mineralnog adsorbenta (I i II grupa) utvrđena je statistička značajnost razlike u koncentraciji IgG u svim ispitivanim intervalima. Koncentracija IgG u krvnom serumu teladi koja su napajana kolostrumom sa dodatkom zeolita (II grupa) bila je oko 30% veća u odnosu na I grupu. Statistički značajna razlika u koncentraciji IgG utvrđena je i poređenjem I grupe sa III i IV u svim ispitivanim intervalima. Koncentracija IgG u krvnom serumu kod III grupe teladi bila je za 40% veća nego u I. Rezultati su ukazali da kod teladi koja su napajana kolostrumom visokog kvaliteta, u koji je dodat mineralni adsorbent, dolazi do maksimalne adsorpcije IgG. Određivanje proteinemije kod teladi u neonatalnom periodu je pouzdan pokazatelj uticaja mineralnog adsorbenta na resorpciju kolostralnih IgG. Koncentracija glukoze kod svih oglednih grupa se značajno povećava sa povećanjem broja uzetih kolostruma, bez većeg uticaja mineralnog adsorbenta.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The effect of mineral adsorbent in calf diet colostrum on the levels of serum immunoglobulin G, protein and glucose
T1  - Efekat mineralnog adsorbenta na nivo serumskih imunoglobulina G, proteina i glukoze kod teladi na kolostralnoj ishrani
VL  - 57
IS  - 2-3
SP  - 169
EP  - 180
DO  - 10.2298/AVB0703169F
ER  - 

@article{
author = "Fratrić, Natalija and Stojić, Velibor and Rajčić, S. and Radojičić, Biljana",
year = "2007",
abstract = "The aim of the study was to investigate the influence of added natural mineral adsorbent zeolite (clinoptylolite) on the degree of absorption of immunoglobulin G and on glucose and protein concentration in the blood of calves during the neonatal period. Sixty calves were randomly divided in to four groups (15 calves per group). The quality and timing of colostrum feeding was as follows: group I was fed only with 1.5 L of colostrum at 2nd, 12th, 24th and 36th hour after birth; group II was fed with 1.5 L of colostrum with the mineral adsorbent added at a 5% concentration in the same time intervals; group III was fed with 1.5 L of colostrum together with the mineral adsorbent added at a 5% concentration, but at 2nd and 12 hour after birth it was fed with the first colostrum, and 24 and 36 hours after birth it was fed with the second colostrum. Group IV of calves was fed the same way as the third one only without the addition of the mineral adsorbent. Mean serum concentration of IgG was 15.14±7.43; 22.22±8.15; 23.65±9.93; 20.81±4.19 g/L in groups I to IV respectively at 6h of age. IgG concentration in the sera of all groups were 29.10±15.25; 45.46±13.45; 50.72±21.57; 41.60±15.42 g/L respectively at 16h after birth. At 30 hours of age, concentration of IgG was 33.29±14.70; 47.05±12.38; 55.00±19.71; 51.59±13.82 g/L in groups I to IV respectively. IgG concentration in the sera of all groups were 29.82±12.69; 44.10±13.50; 51.88±20.14; 47.68±13.18 g/L respectively at 40 hours after birth. For the calves which were fed with the full colostrum without and with the addition of the mineral adsorbent (Group I and II) the statistical significance of the difference in the concentration of IgG was determined during all the tested intervals. Concentration of IgG in the sera of group II calves (fed colostrum with zeolite added) was approximately 30% higher than in the group I. The statistical significance of the difference in levels of IgG between group I and groups III an IV was determined during all tested intervals, also. Concentration of IgG in the sera of group III calves was 40% higher than in group I. Acta Veterinaria (Beograd), Vol. 57, No. 2-3, 169-180, 2007. Results suggest that calves should receive feedings of high quality colostrum with added mineral adsorbent in order to maximize colostral IgG absorption. Determining the proteinemia in the neonatal period of calves is a reliable indicator of the impact of the mineral adsorbent on the degree of absorption of colostral IgG. The concentration of glucose for all the tested groups increased significantly with the increase of the quantity of ingested colostrum, with no evident influence of the added mineral adsorbent., Cilj ovog rada bio je da se ispita uticaj mineralnog adsorbenta na bazi klinoptilolita na stepen resorpcije IgG i koncentraciju nekih biohemijskih parametara u krvnom serumu teladi u neonatalnom periodu. Telad su bila podeljena u četiri grupe (po 15 teladi u svakoj grupi). I grupa teladi napajana je sa kolostrumom 2h, 12h, 24h i 36h posle rođenja, II grupa teladi napajana je sa kolostrumom u koji je dodat mineralni adsorbent u koncentraciji od 5% u istim vremenskim intervalima, III grupa napajana je sa 1,5 L kolostrumom u koji je dodat mineralni adsorbent u koncentraciji od 5% ali 2h i 12h posle rođenja napajana su prvim kolostrumom, a 24h i 36h dugim kolostrumom, IV grupa teladi hranjena je na isti način kao i III grupa samo što u kolostrum nije bio dodat mineralni adsorbent. Koncentracija IgG u krvnom serumu teladi od I do IV grupe, 6 sata iznosila je pojedinačno 15,14±7,43; 22,22±8,15; 23,65±9,93; 20,81±4,19 g/L. U krvnom serumu teladi kod svih ispitivanih grupa koncentracija IgG u 16h iznosila je pojedinač no 29,10±15,25; 45,46±13,45; 50,72±21,57; 41,60±15,42 g/L. Koncentracija IgG 30sata kod ispitivanih grupa teladi (I do IV) bila je pojedinačno 33,29±14,70; 47,05±12,38; 55,00±19,71; 51,59±13,82 g/L. Koncentracija IgG u krvnom serumu svih grupa 40h iznosila je 29,82±12,69; 44,10±13,50; 51,88± 20,14; 47,68±13,18 g/L pojedinačno. Kod teladi koja su napajana punim kolostrumom bez i sa dodatkom mineralnog adsorbenta (I i II grupa) utvrđena je statistička značajnost razlike u koncentraciji IgG u svim ispitivanim intervalima. Koncentracija IgG u krvnom serumu teladi koja su napajana kolostrumom sa dodatkom zeolita (II grupa) bila je oko 30% veća u odnosu na I grupu. Statistički značajna razlika u koncentraciji IgG utvrđena je i poređenjem I grupe sa III i IV u svim ispitivanim intervalima. Koncentracija IgG u krvnom serumu kod III grupe teladi bila je za 40% veća nego u I. Rezultati su ukazali da kod teladi koja su napajana kolostrumom visokog kvaliteta, u koji je dodat mineralni adsorbent, dolazi do maksimalne adsorpcije IgG. Određivanje proteinemije kod teladi u neonatalnom periodu je pouzdan pokazatelj uticaja mineralnog adsorbenta na resorpciju kolostralnih IgG. Koncentracija glukoze kod svih oglednih grupa se značajno povećava sa povećanjem broja uzetih kolostruma, bez većeg uticaja mineralnog adsorbenta.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The effect of mineral adsorbent in calf diet colostrum on the levels of serum immunoglobulin G, protein and glucose, Efekat mineralnog adsorbenta na nivo serumskih imunoglobulina G, proteina i glukoze kod teladi na kolostralnoj ishrani",
volume = "57",
number = "2-3",
pages = "169-180",
doi = "10.2298/AVB0703169F"
}

Fratrić, N., Stojić, V., Rajčić, S.,& Radojičić, B.. (2007). The effect of mineral adsorbent in calf diet colostrum on the levels of serum immunoglobulin G, protein and glucose. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(2-3), 169-180.
https://doi.org/10.2298/AVB0703169F

Fratrić N, Stojić V, Rajčić S, Radojičić B. The effect of mineral adsorbent in calf diet colostrum on the levels of serum immunoglobulin G, protein and glucose. in Acta Veterinaria-Beograd. 2007;57(2-3):169-180.
doi:10.2298/AVB0703169F .

Fratrić, Natalija, Stojić, Velibor, Rajčić, S., Radojičić, Biljana, "The effect of mineral adsorbent in calf diet colostrum on the levels of serum immunoglobulin G, protein and glucose" in Acta Veterinaria-Beograd, 57, no. 2-3 (2007):169-180,
https://doi.org/10.2298/AVB0703169F . .

TY  - JOUR
AU  - Kozmus, P.
AU  - Stevanović, Jevrosima
AU  - Stanimirović, Zoran
AU  - Stojić, Velibor
AU  - Kulišić, Zoran
AU  - Meglič, V.
PY  - 2007
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/468
AB  - In this study mitochondrial DNA of the indigenous honey bee populations from Serbia was analysed. Three ecotypes were comprised in the investigation: Banat (B), Syenichko-Peshterski (S) and Timok (T). The results show that all investigated samples of honey bees from Serbia belong to one mtDNA haplotype of the C phylogenetic lineage, previously designated as C2D haplotype, though with three additional polymorphic sites. For the novel mtDNA haplotype, the designation C2E is proposed. The novel C2E haplotype differs from C2D in two A-T transversions and in one insertion. Moreover, within the T honey bee ecotype, two additional polymorphic sites were observed; a deletion of a T nucleotide and a T-C transition. Our results suggest that the Carniolan honey bee populations from Serbia may represent an indigenous gene pool within A. m. carnica. The mtDNA haplotype (C2D), previously found in A. m. macedonica, was found in all samples of Serbian A. m. carnica. This suggests that the C2D haplotype may be found within both subspecies. This study represents the first effort to characterize the indigenous honey bee populations in Serbia using molecular techniques with the objective of improving our understanding of the phylogeography of Apis mellifera.
AB  - U ovom radu su prikazani rezultati analize mitohondrijalne DNK autohtonih populacija medonosne pčele iz Srbije. Istraživanje je obuhvatilo tri ekotipa: Banatski (B), Sjeničko-Pešterski (S) i Timočki (T). Rezultati ukazuju da svi ispitivani uzorci medonosne pčele iz Srbije pripadaju jednom mtDNK haplotipu C filogenetske linije, ranije označenom kao C2D haplotip, mada sa tri polimorfna mesta više. Za novi mtDNK haplotip predložena je oznaka C2E. Novi C2E haplotip razlikuje se od C2D haplotipa u dve A-T transverzije i jednoj inserciji. Osim toga, u okviru T ekotipa medonosne pčele uočena su još dva polimorfna mesta, delecija nukleotida T i T-C tranzicija. Ovi rezultati ukazuju da populacije kranjske medonosne pčele iz Srbije mogu pretstavljati autohtoni genski pul u okviru podvrste A. m. carnica. Mitohondrijalni DNK haplotip C2D, koji je prethodno opisan kod A. m. macedonica, utvrđen je u svim uzorcima A. m. carnica iz Srbije. Ovakav nalaz sugerise da C2D haplotip može postojati kod obe podvrste. Ovaj rad predstavlja prvi pokušaj da se obavi karakterizacija autohtonih populacija medonosne pčele u Srbiji primenom molekularnih tehnika, a u cilju boljeg razumevanja filogeografije vrste Apis mellifera.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Analysis of mitochondrial DNA in honey bees (Apis mellifera) from Serbia
T1  - Analiza mitohondrijalne DNK kod medonosnih pčela (Apis mellifera) iz Srbije
VL  - 57
IS  - 5-6
SP  - 465
EP  - 476
DO  - 10.2298/AVB0706465K
ER  - 

@article{
author = "Kozmus, P. and Stevanović, Jevrosima and Stanimirović, Zoran and Stojić, Velibor and Kulišić, Zoran and Meglič, V.",
year = "2007",
abstract = "In this study mitochondrial DNA of the indigenous honey bee populations from Serbia was analysed. Three ecotypes were comprised in the investigation: Banat (B), Syenichko-Peshterski (S) and Timok (T). The results show that all investigated samples of honey bees from Serbia belong to one mtDNA haplotype of the C phylogenetic lineage, previously designated as C2D haplotype, though with three additional polymorphic sites. For the novel mtDNA haplotype, the designation C2E is proposed. The novel C2E haplotype differs from C2D in two A-T transversions and in one insertion. Moreover, within the T honey bee ecotype, two additional polymorphic sites were observed; a deletion of a T nucleotide and a T-C transition. Our results suggest that the Carniolan honey bee populations from Serbia may represent an indigenous gene pool within A. m. carnica. The mtDNA haplotype (C2D), previously found in A. m. macedonica, was found in all samples of Serbian A. m. carnica. This suggests that the C2D haplotype may be found within both subspecies. This study represents the first effort to characterize the indigenous honey bee populations in Serbia using molecular techniques with the objective of improving our understanding of the phylogeography of Apis mellifera., U ovom radu su prikazani rezultati analize mitohondrijalne DNK autohtonih populacija medonosne pčele iz Srbije. Istraživanje je obuhvatilo tri ekotipa: Banatski (B), Sjeničko-Pešterski (S) i Timočki (T). Rezultati ukazuju da svi ispitivani uzorci medonosne pčele iz Srbije pripadaju jednom mtDNK haplotipu C filogenetske linije, ranije označenom kao C2D haplotip, mada sa tri polimorfna mesta više. Za novi mtDNK haplotip predložena je oznaka C2E. Novi C2E haplotip razlikuje se od C2D haplotipa u dve A-T transverzije i jednoj inserciji. Osim toga, u okviru T ekotipa medonosne pčele uočena su još dva polimorfna mesta, delecija nukleotida T i T-C tranzicija. Ovi rezultati ukazuju da populacije kranjske medonosne pčele iz Srbije mogu pretstavljati autohtoni genski pul u okviru podvrste A. m. carnica. Mitohondrijalni DNK haplotip C2D, koji je prethodno opisan kod A. m. macedonica, utvrđen je u svim uzorcima A. m. carnica iz Srbije. Ovakav nalaz sugerise da C2D haplotip može postojati kod obe podvrste. Ovaj rad predstavlja prvi pokušaj da se obavi karakterizacija autohtonih populacija medonosne pčele u Srbiji primenom molekularnih tehnika, a u cilju boljeg razumevanja filogeografije vrste Apis mellifera.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Analysis of mitochondrial DNA in honey bees (Apis mellifera) from Serbia, Analiza mitohondrijalne DNK kod medonosnih pčela (Apis mellifera) iz Srbije",
volume = "57",
number = "5-6",
pages = "465-476",
doi = "10.2298/AVB0706465K"
}

Kozmus, P., Stevanović, J., Stanimirović, Z., Stojić, V., Kulišić, Z.,& Meglič, V.. (2007). Analysis of mitochondrial DNA in honey bees (Apis mellifera) from Serbia. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 57(5-6), 465-476.
https://doi.org/10.2298/AVB0706465K

Kozmus P, Stevanović J, Stanimirović Z, Stojić V, Kulišić Z, Meglič V. Analysis of mitochondrial DNA in honey bees (Apis mellifera) from Serbia. in Acta Veterinaria-Beograd. 2007;57(5-6):465-476.
doi:10.2298/AVB0706465K .

Kozmus, P., Stevanović, Jevrosima, Stanimirović, Zoran, Stojić, Velibor, Kulišić, Zoran, Meglič, V., "Analysis of mitochondrial DNA in honey bees (Apis mellifera) from Serbia" in Acta Veterinaria-Beograd, 57, no. 5-6 (2007):465-476,
https://doi.org/10.2298/AVB0706465K . .

TY  - JOUR
AU  - Fratrić, Natalija
AU  - Milošević-Jovčić, Nadežda
AU  - Ilić, Vesna
AU  - Stojić, Velibor
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/376
AB  - In this work immune complexes (IC) in the sera of healthy calves from birth to four months of age, heifers and cows were investigated. Polyethylen glycol (PEG) precipitation assay was applied after previous standardization. Optical density (OD) of redissolved precipitates, formed after adding PEG to serum samples, was a measure of IC level. The upper-limit values for normal IC levels were established using isolated cattle IgG in different amounts and aggregated by heat as an IC model in vitro, in the same assay. In newborn calves before colostrum intake, OD of 0.047±0.024 was registered in redissolved PEG precipitates, far below the values obtained for native monomeric IgG. Therefore, these values can not be considered to refer to immune complexes. After colostrum intake, OD values for normal IC levels were higher, with significant interindividual differences: in calves at 6 24 and 48 hours after birth the mean OD values of solubilized PEG precipitates were 0.069±0.025, 0.148±0.079, and 0.136±0.062 respectively. In the sera of calves 10 days after birth mean OD value was slightly lower 0.089±0.053, whereas in the serum of 1, 2, 3 and 4 month old calves normal levels of IC rose to 0.165±0.067, 0.157±0.080, 0,231±0,124 and 0.261±0.092 respectively. OD values for IC levels for adult animals were found to be 0,456±0,150 for heifers, and 0,183±0,031 for cows. Together, the obtained data may be included in a reference values system for normal levels of IC at different ages, in relation to which the IC levels under pathological conditions could be estimated.
AB  - U radu je ispitivan nivo imunokompleksa (IC) u krvnom serumu teladi od rođenja do četiri meseca starosti, kao i kod junica i krava. Za analize je posle predhodne standardizacije korišćen polietilenglikol precipitacioni test (direktan PEG test). Meru nivoa IC predstavljale su vrednosti optičke gustine (OD) rastvorenog precipitata dobijenog nakon taloženja imunokompleksa iz seruma PEG-om. Gornja granica normalnih vrednosti za nivo IC utvrđena je prema vrednostima dobijenim za izolovane i toplotom agregirane kravlje IgG, u istom testu, gde su takvi IgG korišćeni kao modeli IC in vitro. U grupi novorođene teladi pre uzimanja kolostruma vrednosti OD su bile ekstremno niske (OD 0,047±0.024) i nisu mogle biti pripisane imunokompleksima. Vrednosti OD za normalan nivo IC u serumu teladi nakon uzimanja kolostruma su bile više: za telad 6, 24 i 48 sati nakon rođenja taj nivo je iznosio 0,069±0,025, 0,148±0,079 i 0,136±0,062 respektivno. U odnosu na ove vrednosti, nivo IC u serumu teladi 10 dana od rođenja je bio nešto niži i iznosio 0,089±0,053, da bi se, zatim, kontinuirano povećavao kod teladi uzrasta od 1 do 4 meseca i iznosio 0,165±0,067 za telad od 1-og meseca, 0,157±0,080 za telad od 2 meseca, 0,231±0,124 za telad od 3 meseca i 0,261±0,092 za telad od 4 meseca starosti. Nivo imunokompleksa u serumu odraslih goveda iznosio je kod junica 0,456±0,150 i krava 0,183±0,031. Dobijeni rezultati mogu biti uključeni u kontrolni sistem vrednosti za normalan nivo IC u raznim starosnim grupama goveda, koji se može koristiti pri određivanju nivoa IC PEG testom pod patološkim uslovima kod teladi junica i krava.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - The levels of immune complexes in the blood sera of calves in the neonatal period and in adult cattle
T1  - Imunokompleksi u krvnom serumu teladi u neonatalnom periodu i kod odraslih goveda
VL  - 56
IS  - 2-3
SP  - 103
EP  - 110
DO  - 10.2298/AVB0603103F
ER  - 

@article{
author = "Fratrić, Natalija and Milošević-Jovčić, Nadežda and Ilić, Vesna and Stojić, Velibor",
year = "2006",
abstract = "In this work immune complexes (IC) in the sera of healthy calves from birth to four months of age, heifers and cows were investigated. Polyethylen glycol (PEG) precipitation assay was applied after previous standardization. Optical density (OD) of redissolved precipitates, formed after adding PEG to serum samples, was a measure of IC level. The upper-limit values for normal IC levels were established using isolated cattle IgG in different amounts and aggregated by heat as an IC model in vitro, in the same assay. In newborn calves before colostrum intake, OD of 0.047±0.024 was registered in redissolved PEG precipitates, far below the values obtained for native monomeric IgG. Therefore, these values can not be considered to refer to immune complexes. After colostrum intake, OD values for normal IC levels were higher, with significant interindividual differences: in calves at 6 24 and 48 hours after birth the mean OD values of solubilized PEG precipitates were 0.069±0.025, 0.148±0.079, and 0.136±0.062 respectively. In the sera of calves 10 days after birth mean OD value was slightly lower 0.089±0.053, whereas in the serum of 1, 2, 3 and 4 month old calves normal levels of IC rose to 0.165±0.067, 0.157±0.080, 0,231±0,124 and 0.261±0.092 respectively. OD values for IC levels for adult animals were found to be 0,456±0,150 for heifers, and 0,183±0,031 for cows. Together, the obtained data may be included in a reference values system for normal levels of IC at different ages, in relation to which the IC levels under pathological conditions could be estimated., U radu je ispitivan nivo imunokompleksa (IC) u krvnom serumu teladi od rođenja do četiri meseca starosti, kao i kod junica i krava. Za analize je posle predhodne standardizacije korišćen polietilenglikol precipitacioni test (direktan PEG test). Meru nivoa IC predstavljale su vrednosti optičke gustine (OD) rastvorenog precipitata dobijenog nakon taloženja imunokompleksa iz seruma PEG-om. Gornja granica normalnih vrednosti za nivo IC utvrđena je prema vrednostima dobijenim za izolovane i toplotom agregirane kravlje IgG, u istom testu, gde su takvi IgG korišćeni kao modeli IC in vitro. U grupi novorođene teladi pre uzimanja kolostruma vrednosti OD su bile ekstremno niske (OD 0,047±0.024) i nisu mogle biti pripisane imunokompleksima. Vrednosti OD za normalan nivo IC u serumu teladi nakon uzimanja kolostruma su bile više: za telad 6, 24 i 48 sati nakon rođenja taj nivo je iznosio 0,069±0,025, 0,148±0,079 i 0,136±0,062 respektivno. U odnosu na ove vrednosti, nivo IC u serumu teladi 10 dana od rođenja je bio nešto niži i iznosio 0,089±0,053, da bi se, zatim, kontinuirano povećavao kod teladi uzrasta od 1 do 4 meseca i iznosio 0,165±0,067 za telad od 1-og meseca, 0,157±0,080 za telad od 2 meseca, 0,231±0,124 za telad od 3 meseca i 0,261±0,092 za telad od 4 meseca starosti. Nivo imunokompleksa u serumu odraslih goveda iznosio je kod junica 0,456±0,150 i krava 0,183±0,031. Dobijeni rezultati mogu biti uključeni u kontrolni sistem vrednosti za normalan nivo IC u raznim starosnim grupama goveda, koji se može koristiti pri određivanju nivoa IC PEG testom pod patološkim uslovima kod teladi junica i krava.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "The levels of immune complexes in the blood sera of calves in the neonatal period and in adult cattle, Imunokompleksi u krvnom serumu teladi u neonatalnom periodu i kod odraslih goveda",
volume = "56",
number = "2-3",
pages = "103-110",
doi = "10.2298/AVB0603103F"
}

Fratrić, N., Milošević-Jovčić, N., Ilić, V.,& Stojić, V.. (2006). The levels of immune complexes in the blood sera of calves in the neonatal period and in adult cattle. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(2-3), 103-110.
https://doi.org/10.2298/AVB0603103F

Fratrić N, Milošević-Jovčić N, Ilić V, Stojić V. The levels of immune complexes in the blood sera of calves in the neonatal period and in adult cattle. in Acta Veterinaria-Beograd. 2006;56(2-3):103-110.
doi:10.2298/AVB0603103F .

Fratrić, Natalija, Milošević-Jovčić, Nadežda, Ilić, Vesna, Stojić, Velibor, "The levels of immune complexes in the blood sera of calves in the neonatal period and in adult cattle" in Acta Veterinaria-Beograd, 56, no. 2-3 (2006):103-110,
https://doi.org/10.2298/AVB0603103F . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Vujanac, Ivan
AU  - Stojić, Velibor
AU  - Ivanov, Ivan
AU  - Adamović, Milan
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/435
AB  - In this paper we presented recent results about initiation and consequences of acid indigestion, especially in high yielding lactating cows in first two to three months after calving. It is pointed to the consequences of acid indigestions, and one of them is aseptic sub dermatitis which cause serious economic loses in cattle production. In this paper, it is presented that this problem could be resolved using mineral mixtures with buffering activity, prepared on bentonite, zeolite, magnesium oxide and sodium bicarbonate basis. Supplementation of this mineral mixture, in amount of 1%, beside prevention of rumen acidosis, significantly reduced frequency of aseptic sub dermatitis incidence, and increased milk production and milk fat content.
AB  - U radu se razmatraju novija saznanja o uzrocima nastanka i posledicama do kojih dolazi usled kiselih indigestija goveda, u prvom redu kod visokoproizvodnih krava u prva dva do tri meseca posle teljenja. Skrenuta je pažnja da su posledice kiselih indigestija višestruke, a jedna od njih je i aseptični pododermatitis koji nanosi značajne ekonomske štete u govedarskoj proizvodnji. Ukazano je da se ovaj problem može rešavati korišćenjem mineralnih smeša sa pufernim dejstvom, na bazi bentonita, zeolita, magnezijum oksida i natrijum bikarbonata. Uključivanje ove mineralne smeše u koncentrat (u količini od 1%) pored preveniranja acidoze buraga značajno je doprinelo smanjenju učestalosti aseptičnog pododermatitisa kao i povećanju količine mleka i sadržaja masti u mleku.
PB  - Institut PKB Agroekonomik, Padinska skela
T2  - Zbornik naučnih radova Instituta PKB Agroekonomik
T1  - Prevention of acid indigestions in lactating cows
T1  - Preveniranje kiselih indigestija goveda
VL  - 12
IS  - 3-4
SP  - 37
EP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_veterinar_435
ER  - 

@article{
author = "Šamanc, Horea and Vujanac, Ivan and Stojić, Velibor and Ivanov, Ivan and Adamović, Milan",
year = "2006",
abstract = "In this paper we presented recent results about initiation and consequences of acid indigestion, especially in high yielding lactating cows in first two to three months after calving. It is pointed to the consequences of acid indigestions, and one of them is aseptic sub dermatitis which cause serious economic loses in cattle production. In this paper, it is presented that this problem could be resolved using mineral mixtures with buffering activity, prepared on bentonite, zeolite, magnesium oxide and sodium bicarbonate basis. Supplementation of this mineral mixture, in amount of 1%, beside prevention of rumen acidosis, significantly reduced frequency of aseptic sub dermatitis incidence, and increased milk production and milk fat content., U radu se razmatraju novija saznanja o uzrocima nastanka i posledicama do kojih dolazi usled kiselih indigestija goveda, u prvom redu kod visokoproizvodnih krava u prva dva do tri meseca posle teljenja. Skrenuta je pažnja da su posledice kiselih indigestija višestruke, a jedna od njih je i aseptični pododermatitis koji nanosi značajne ekonomske štete u govedarskoj proizvodnji. Ukazano je da se ovaj problem može rešavati korišćenjem mineralnih smeša sa pufernim dejstvom, na bazi bentonita, zeolita, magnezijum oksida i natrijum bikarbonata. Uključivanje ove mineralne smeše u koncentrat (u količini od 1%) pored preveniranja acidoze buraga značajno je doprinelo smanjenju učestalosti aseptičnog pododermatitisa kao i povećanju količine mleka i sadržaja masti u mleku.",
publisher = "Institut PKB Agroekonomik, Padinska skela",
journal = "Zbornik naučnih radova Instituta PKB Agroekonomik",
title = "Prevention of acid indigestions in lactating cows, Preveniranje kiselih indigestija goveda",
volume = "12",
number = "3-4",
pages = "37-45",
url = "https://hdl.handle.net/21.15107/rcub_veterinar_435"
}

Šamanc, H., Vujanac, I., Stojić, V., Ivanov, I.,& Adamović, M.. (2006). Prevention of acid indigestions in lactating cows. in Zbornik naučnih radova Instituta PKB Agroekonomik
Institut PKB Agroekonomik, Padinska skela., 12(3-4), 37-45.
https://hdl.handle.net/21.15107/rcub_veterinar_435

Šamanc H, Vujanac I, Stojić V, Ivanov I, Adamović M. Prevention of acid indigestions in lactating cows. in Zbornik naučnih radova Instituta PKB Agroekonomik. 2006;12(3-4):37-45.
https://hdl.handle.net/21.15107/rcub_veterinar_435 .

Šamanc, Horea, Vujanac, Ivan, Stojić, Velibor, Ivanov, Ivan, Adamović, Milan, "Prevention of acid indigestions in lactating cows" in Zbornik naučnih radova Instituta PKB Agroekonomik, 12, no. 3-4 (2006):37-45,
https://hdl.handle.net/21.15107/rcub_veterinar_435 .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Stojić, Velibor
AU  - Vujanac, Ivan
AU  - Dimitrijević, Blagoje
AU  - Huszenicza, Gyula
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/370
AB  - From the very onset of lactation, the requirements of the mammary gland have an advantage over the requirements of other organic systems, even in the case of clinically exhibited ketosis. In hypoglycemic, ketotic cows, the mammary gland utilizes glucosis approximately in the same degree as the mammary gland of cows with normal glycemia. This indicates that the regulatory role of hormones can have primary importance in the earliest phase of lactation, when metabolic processes are on the very brink of a ketotic state. That is why it is believed that, in conditions of a great burdening of the metabolism, inadequate activity by the adrenocortical system presents one of the primary factors in the etiopathogenesis of ketosis in dairy cows. It is still an open issue whether hypof unction of the adrenal cortex is a direct cause of this disorder, or whether its activity is a consequence of insufficient stimulation by ACTH. In ketotic cows, average concentrations of cortisol have been established in ketotic cows, from 23.98 nmol/l in blood serum, and it is approximately the same as in healthy cows in the corresponding phase of lactation. However, the vast range of individual values largely makes it difficult to make a correct evaluation of the functional state of the adrenal cortex of ketotic cows. On the grounds of individual figures, in 26% diseased cows cortisol concentration in blood serum is from 1.38 to 16.55 nmol/l, in 37% it is from 16.55 to 27.59 nmol/l, and in 37 percent it is over 27.59 nmol/l. This indicates that cows with clinical symptoms of ketosis can be divided into those with low, medium, or high values of cortisol concentration in blood serum. Following the stimulation of the adrenal cortex with ACTH, the individual differences in serum cortisol concentration are considerably decreased. The ratio between the lowest and the highest values of cortisol concentration before the application of ACTH is 1:6, while it is only 1:1.5 four hours following application. After that, the values again take on the starting characteristics, and 24 hours after the application of ACTH they return to the starting values, and the ratio between the individual values is 1:8. It seems that the function of the adrenal cortex has been preserved in cows with clinical symptoms of ketosis and it is capable of reacting fully to stimulation with corticotropin. The cortisolemia values indicate, primarily, that there are big individual differences regarding the regulation of the degree of activity of the adrenal cortex, so that it is possible that, in the ketotic state, it is rather a case of uncoordinated activities of the adrenal cortex with the organism requirements imposed by the mammary gland, than that it is a matter of its hypofunction. .
AB  - Od samog početka laktacije potrebe mlečne žlezde imaju prednost u odnosu na potrebe drugih organskih sistema, čak i u slučaju klinički ispoljene ketoze. Kod hipoglikemičnih, ketoznih krava, mlečna žlezda koristi glikozu u približno istom obimu kao i mlečna žlezda krava sa normalnom glikemijom. To ukazuje da u najranijoj fazi laktacije, kada su metabolički procesi na samoj granici ketoznog stanja, regulatorna uloga hormona može da ima prvostepeni značaj. Zbog toga se smatra da u uslovima velikog opterećenja metabolizma neadekvatna aktivnost adrenokortikalnog sistema predstavlja jedan od primarnih činilaca u etiopatogenezi ketoze mlečnih krava. Još uvek je otvoreno pitanje da li je hipofunkcija kore nadbubrega neposredan uzrok ovog poremećaja ili je njena aktivnost posledica nedovoljne stimulacije ACTH. Kod ketoznih krava je utvrđeno da je prosečna koncentracija kortizola od 23,98 nmol/l u krvnom serumu i približno je ista kao i kod zdravih krava u istoj fazi laktacije. Međutim, veliki raspon pojedinačnih vrednosti, u velikoj meri otežava pravilnu procenu funkcionalnog stanja kore nadbubrega kod ketoznih krava. Na osnovu pojedinačnih podataka kod 26 posto obolelih krava koncentracija kortizola u krvnom serumu je od 1,38 do 16,55 nmol/l, kod 37posto od 16,55 do 27,59 nmol/l, a kod 37 posto više od 27,59 nmol/l. To ukazuje da se krave sa kliničkim simptomima ketoze mogu da svrstaju na one sa niskim, srednjim i visokim vrednostima koncentracije kortizola u krvnom serumu. Nakon stimulisanja kore nadbubrega sa ACTH znatno se smanjuju pojedinačne razlike u koncentraciji serumskog kortizola. Odnos između najniže i najviše vrednosti koncentracije kortizola pre aplikovanja ACTH je 1:6, a četiri časa posle aplikacije je samo 1:1,5. Zatim, vrednosti ponovo poprimaju početna obeležja i nakon 24 časa od primene ACTH vraćaju se na početne vrednosti, a raspon pojedinačnih vrednosti je 1:8. Po svemu sudeći očuvana je funkcija kore nadbubrega kod krava sa kliničkim simptomima ketoze i u stanju je da u potpunosti reaguje na stimulaciju kortikotropinom. Vrednosti kortizolemije ukazuju, pre svega, da postoje velike pojedinačne razlike u pogledu regulacije stepena aktivnosti kore nadbubrega, tako daje moguće da se u ketoznom stanju više radi o neusklađenoj aktivnosti kore nadbubrega prema potrebama organizma koje nameće mlečna žlezda, nego što se radi o njenoj hipofunkciji. .
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Role of adrenocortical system in occurrence of ketosis in high-yield dairy cows
T1  - Uloga adrenokortikalnog sistema u nastanku ketoze visoko-mlečnih krava
VL  - 60
IS  - 3-4
SP  - 125
EP  - 134
DO  - 10.2298/VETGL0604125S
ER  - 

@article{
author = "Šamanc, Horea and Stojić, Velibor and Vujanac, Ivan and Dimitrijević, Blagoje and Huszenicza, Gyula",
year = "2006",
abstract = "From the very onset of lactation, the requirements of the mammary gland have an advantage over the requirements of other organic systems, even in the case of clinically exhibited ketosis. In hypoglycemic, ketotic cows, the mammary gland utilizes glucosis approximately in the same degree as the mammary gland of cows with normal glycemia. This indicates that the regulatory role of hormones can have primary importance in the earliest phase of lactation, when metabolic processes are on the very brink of a ketotic state. That is why it is believed that, in conditions of a great burdening of the metabolism, inadequate activity by the adrenocortical system presents one of the primary factors in the etiopathogenesis of ketosis in dairy cows. It is still an open issue whether hypof unction of the adrenal cortex is a direct cause of this disorder, or whether its activity is a consequence of insufficient stimulation by ACTH. In ketotic cows, average concentrations of cortisol have been established in ketotic cows, from 23.98 nmol/l in blood serum, and it is approximately the same as in healthy cows in the corresponding phase of lactation. However, the vast range of individual values largely makes it difficult to make a correct evaluation of the functional state of the adrenal cortex of ketotic cows. On the grounds of individual figures, in 26% diseased cows cortisol concentration in blood serum is from 1.38 to 16.55 nmol/l, in 37% it is from 16.55 to 27.59 nmol/l, and in 37 percent it is over 27.59 nmol/l. This indicates that cows with clinical symptoms of ketosis can be divided into those with low, medium, or high values of cortisol concentration in blood serum. Following the stimulation of the adrenal cortex with ACTH, the individual differences in serum cortisol concentration are considerably decreased. The ratio between the lowest and the highest values of cortisol concentration before the application of ACTH is 1:6, while it is only 1:1.5 four hours following application. After that, the values again take on the starting characteristics, and 24 hours after the application of ACTH they return to the starting values, and the ratio between the individual values is 1:8. It seems that the function of the adrenal cortex has been preserved in cows with clinical symptoms of ketosis and it is capable of reacting fully to stimulation with corticotropin. The cortisolemia values indicate, primarily, that there are big individual differences regarding the regulation of the degree of activity of the adrenal cortex, so that it is possible that, in the ketotic state, it is rather a case of uncoordinated activities of the adrenal cortex with the organism requirements imposed by the mammary gland, than that it is a matter of its hypofunction. ., Od samog početka laktacije potrebe mlečne žlezde imaju prednost u odnosu na potrebe drugih organskih sistema, čak i u slučaju klinički ispoljene ketoze. Kod hipoglikemičnih, ketoznih krava, mlečna žlezda koristi glikozu u približno istom obimu kao i mlečna žlezda krava sa normalnom glikemijom. To ukazuje da u najranijoj fazi laktacije, kada su metabolički procesi na samoj granici ketoznog stanja, regulatorna uloga hormona može da ima prvostepeni značaj. Zbog toga se smatra da u uslovima velikog opterećenja metabolizma neadekvatna aktivnost adrenokortikalnog sistema predstavlja jedan od primarnih činilaca u etiopatogenezi ketoze mlečnih krava. Još uvek je otvoreno pitanje da li je hipofunkcija kore nadbubrega neposredan uzrok ovog poremećaja ili je njena aktivnost posledica nedovoljne stimulacije ACTH. Kod ketoznih krava je utvrđeno da je prosečna koncentracija kortizola od 23,98 nmol/l u krvnom serumu i približno je ista kao i kod zdravih krava u istoj fazi laktacije. Međutim, veliki raspon pojedinačnih vrednosti, u velikoj meri otežava pravilnu procenu funkcionalnog stanja kore nadbubrega kod ketoznih krava. Na osnovu pojedinačnih podataka kod 26 posto obolelih krava koncentracija kortizola u krvnom serumu je od 1,38 do 16,55 nmol/l, kod 37posto od 16,55 do 27,59 nmol/l, a kod 37 posto više od 27,59 nmol/l. To ukazuje da se krave sa kliničkim simptomima ketoze mogu da svrstaju na one sa niskim, srednjim i visokim vrednostima koncentracije kortizola u krvnom serumu. Nakon stimulisanja kore nadbubrega sa ACTH znatno se smanjuju pojedinačne razlike u koncentraciji serumskog kortizola. Odnos između najniže i najviše vrednosti koncentracije kortizola pre aplikovanja ACTH je 1:6, a četiri časa posle aplikacije je samo 1:1,5. Zatim, vrednosti ponovo poprimaju početna obeležja i nakon 24 časa od primene ACTH vraćaju se na početne vrednosti, a raspon pojedinačnih vrednosti je 1:8. Po svemu sudeći očuvana je funkcija kore nadbubrega kod krava sa kliničkim simptomima ketoze i u stanju je da u potpunosti reaguje na stimulaciju kortikotropinom. Vrednosti kortizolemije ukazuju, pre svega, da postoje velike pojedinačne razlike u pogledu regulacije stepena aktivnosti kore nadbubrega, tako daje moguće da se u ketoznom stanju više radi o neusklađenoj aktivnosti kore nadbubrega prema potrebama organizma koje nameće mlečna žlezda, nego što se radi o njenoj hipofunkciji. .",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Role of adrenocortical system in occurrence of ketosis in high-yield dairy cows, Uloga adrenokortikalnog sistema u nastanku ketoze visoko-mlečnih krava",
volume = "60",
number = "3-4",
pages = "125-134",
doi = "10.2298/VETGL0604125S"
}

Šamanc, H., Stojić, V., Vujanac, I., Dimitrijević, B.,& Huszenicza, G.. (2006). Role of adrenocortical system in occurrence of ketosis in high-yield dairy cows. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(3-4), 125-134.
https://doi.org/10.2298/VETGL0604125S

Šamanc H, Stojić V, Vujanac I, Dimitrijević B, Huszenicza G. Role of adrenocortical system in occurrence of ketosis in high-yield dairy cows. in Veterinarski Glasnik. 2006;60(3-4):125-134.
doi:10.2298/VETGL0604125S .

Šamanc, Horea, Stojić, Velibor, Vujanac, Ivan, Dimitrijević, Blagoje, Huszenicza, Gyula, "Role of adrenocortical system in occurrence of ketosis in high-yield dairy cows" in Veterinarski Glasnik, 60, no. 3-4 (2006):125-134,
https://doi.org/10.2298/VETGL0604125S . .

TY  - JOUR
AU  - Šamanc, Horea
AU  - Stojić, Velibor
AU  - Adamović, Milan
AU  - Vujanac, Ivan
AU  - Petrujkić, Branko
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/377
AB  - Rumen acidosis is a very important pathophysiological disorder in intensive productive dairy cows, it is mostly a problem in early lactation when highly concentrated feeds are used in nutrition. The use of mineral salt mix based on bentonite, zeolite, magnesium oxide and sodium bicarbonate stabilizes and maintains pH of ruminal fluid in physiological values (6,79 to 6,92) and prevents the occurrence of rumen acidosis. In cows in the control group, the pH of ruminal fluid was at a low physiological level (6.01 to 6.25) and in some animals even lower. By adding this mixture of mineral salts to the concentrated part of feed (1%) optimal conditions are provided for activity and adequate numerous distribution of all species of infusoria in the rumen. The daily amount of milk produced and milk fat as well was 10 percent bigger in the experimental compared to the control group of cows.
AB  - Poremećaji u procesima razlaganja hrane u predželucima i nastala acidoza prouzrokuju mnogobrojne patološke procese, uključujući i morfološke promene sluzokože buraga. Prvo se smanjuje motorička aktivnost predželudaca i usporava se pasaža sadržaja. Zatim nastaju ruminitis i parakeratoza. Kao posledica toga značajno se smanjuje resorptivna površina sluzokože buraga i stepen resorpcije proizvoda razlaganja hrane. Njihov smanjen priliv može da naruši metaboličku ravnotežu, pre svega stabilnost energetskog metabolizma. Imajući u vidu da je kod acidoze buraga smanjen apetit, onda je sasvim razumljivo što je krajnji rezultat ovih poremećaja smanjena proizvodnja mleka. U ovom radu se razmatraju novija saznanja o uzrocima nastanka i posledicama koje nastaju usled acidoze buraga, pre svega visoko proizvodnih krava u prva dva do tri meseca laktacije. Utvrđeno je da se acidoza buraga može uspešno da prevenira korišćenjem mineralnih smeša sa pufernim dejstvom, na bazi bentonita, zeolita, magnezijum oksida i natrijum bikarbonata (Mix Plus). Uključivanje ove mineralne smeše u koncentrat (u količini od 1%) pored preveniranja acidoze buraga doprinosi povećanju količine mleka i sadržaja masti u mleku.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski Glasnik
T1  - Rumen acidosis: Possibilities of prevention using of mineral mix with buffering effect
T1  - Acidoza buraga: mogućnost preventiranja korišćenjem mineralnih smeša sa pufernim dejstvom
VL  - 60
IS  - 1-2
SP  - 11
EP  - 19
DO  - 10.2298/VETGL0602011S
ER  - 

@article{
author = "Šamanc, Horea and Stojić, Velibor and Adamović, Milan and Vujanac, Ivan and Petrujkić, Branko",
year = "2006",
abstract = "Rumen acidosis is a very important pathophysiological disorder in intensive productive dairy cows, it is mostly a problem in early lactation when highly concentrated feeds are used in nutrition. The use of mineral salt mix based on bentonite, zeolite, magnesium oxide and sodium bicarbonate stabilizes and maintains pH of ruminal fluid in physiological values (6,79 to 6,92) and prevents the occurrence of rumen acidosis. In cows in the control group, the pH of ruminal fluid was at a low physiological level (6.01 to 6.25) and in some animals even lower. By adding this mixture of mineral salts to the concentrated part of feed (1%) optimal conditions are provided for activity and adequate numerous distribution of all species of infusoria in the rumen. The daily amount of milk produced and milk fat as well was 10 percent bigger in the experimental compared to the control group of cows., Poremećaji u procesima razlaganja hrane u predželucima i nastala acidoza prouzrokuju mnogobrojne patološke procese, uključujući i morfološke promene sluzokože buraga. Prvo se smanjuje motorička aktivnost predželudaca i usporava se pasaža sadržaja. Zatim nastaju ruminitis i parakeratoza. Kao posledica toga značajno se smanjuje resorptivna površina sluzokože buraga i stepen resorpcije proizvoda razlaganja hrane. Njihov smanjen priliv može da naruši metaboličku ravnotežu, pre svega stabilnost energetskog metabolizma. Imajući u vidu da je kod acidoze buraga smanjen apetit, onda je sasvim razumljivo što je krajnji rezultat ovih poremećaja smanjena proizvodnja mleka. U ovom radu se razmatraju novija saznanja o uzrocima nastanka i posledicama koje nastaju usled acidoze buraga, pre svega visoko proizvodnih krava u prva dva do tri meseca laktacije. Utvrđeno je da se acidoza buraga može uspešno da prevenira korišćenjem mineralnih smeša sa pufernim dejstvom, na bazi bentonita, zeolita, magnezijum oksida i natrijum bikarbonata (Mix Plus). Uključivanje ove mineralne smeše u koncentrat (u količini od 1%) pored preveniranja acidoze buraga doprinosi povećanju količine mleka i sadržaja masti u mleku.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski Glasnik",
title = "Rumen acidosis: Possibilities of prevention using of mineral mix with buffering effect, Acidoza buraga: mogućnost preventiranja korišćenjem mineralnih smeša sa pufernim dejstvom",
volume = "60",
number = "1-2",
pages = "11-19",
doi = "10.2298/VETGL0602011S"
}

Šamanc, H., Stojić, V., Adamović, M., Vujanac, I.,& Petrujkić, B.. (2006). Rumen acidosis: Possibilities of prevention using of mineral mix with buffering effect. in Veterinarski Glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 60(1-2), 11-19.
https://doi.org/10.2298/VETGL0602011S

Šamanc H, Stojić V, Adamović M, Vujanac I, Petrujkić B. Rumen acidosis: Possibilities of prevention using of mineral mix with buffering effect. in Veterinarski Glasnik. 2006;60(1-2):11-19.
doi:10.2298/VETGL0602011S .

Šamanc, Horea, Stojić, Velibor, Adamović, Milan, Vujanac, Ivan, Petrujkić, Branko, "Rumen acidosis: Possibilities of prevention using of mineral mix with buffering effect" in Veterinarski Glasnik, 60, no. 1-2 (2006):11-19,
https://doi.org/10.2298/VETGL0602011S . .

TY  - JOUR
AU  - Stanimirović, Zoran
AU  - Stevanović, Jevrosima
AU  - Kulić, Milan
AU  - Stojić, Velibor
PY  - 2006
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/423
AB  - Dicyclohexylamine (fumagillin), as an antibiotic produced by fermentation of Aspergillus fumigatus, is used in human medicine for the treatment of intestinal microsporidiosis in patients with HIV infection, intestinal amebiasis and microsporidial keratoconjunctivitis. In veterinary medicine fumagillin is effective in suppressing microsporidiosis of bees and fish. In this study, the genotoxicity of fumagillin was evaluated in mouse bone marrow cells using chromosome aberrations (CA) assay. Dicyclohexylamine was administered to mice by gavage in a dose of 25, 50, 75 mg/kg b.w., with water-sugar syrup as the negative control and cyclophosphamide as the positive control (40 mg/kg b.w) Significantly increased frequency (p lt 0.01 or p lt 0.001) of numerical chromosomal aberrations (aneupliodies and polyploidies) and structural chromosomal aberrations of gaps, breaks and centric rings were observed only at the highest experimental dose of dicyclohexylamine, compared with the negative control. However, in point of induction of Rb translocations, both the median (50 mg/kg b.w) and highest (75 mg/kg b.w) experimental dose showed a significant (p lt 00.001) increase (7.12 ± 0.26 and 9.00 ± 0.10, respectively) in comparison with the negative control (0.00 ± 0.00). Chromosomes 4 and 19 participated in these Rb translocations. These results suggest that dicyclohexilamine (fumagillin) has genotoxic potential in mammal in vivo chromosomal aberration (CA) test system.
AB  - Dicyclohexylamin (fumagilin), kao antibiotik koga proizvodi gljivica Aspergillus fumigatus, se koristi u humanoj medicini u borbi protiv intestinalnih mikrosporidioza kod pacijenata sa HIV infekcijom, ali i za tretman obolelih od intestinalnih amebijaza i mikrosporidijanog keratokonjunktivitisa. U veterinarskoj medicini efikasan je u lečenju mikrosporidioza pčela i drugih insekata uzrokovanih sa Nosema apis, N. cerani, N. bombici, ali i mikrosporidioza riba uzrokovanih sa Myxobolus cerebralis i Tetracapsuloides bryosalmonae. U ovom radu ispitivana je genotoksičnost dicikloheksilamina kvantifikacijom i kvalifikacijom hromozomskih aberacija u ćelijama kostne srži miševa soja BALB/c. Dicikloheksilamin je aplikovan miševima intragastrično u dozama od 25, 50, 75 mg/kg b.w., pri čemu je vodeno-šećerni sirup predstavljao negativnu, a cyclophosphamid (40 mg/kg b.w) poozitivnu kontrolu. Utvrđeno je da samo najveća doza dicikloheksilamina signifikantno povećava učestalost (p lt 0.01 ili p lt 0.001) numeričkih hromozomskih aberacija (aneupliodija i poliploidija) i strukturnih hromozomskih aberacija tipa gapova, prekida i centričnih ringova u poređenju sa negativnom kontrolom. Međutim, srednja (50 mg/kg b.w) i najviša (75 mg/kg b.w) eksperimentalna doza izazvale su signifikantno (p lt 0.001) povećanje (7.12 ± 0.26 i 9.00 ± 0.10, respektivno) Robetrsonovih (Rb) translokacija u poređenju sa negativnom kontrolom (0.00 ± 0.00). U formiranju ovih Rb translokacija utvrđeno je da učestvuju hromozomi 4 i 19. Ovi rezultati sugerišu da je dicikloheksilamin (fumagilin) ispoljio visok genotoksični potencijal sa sposobnošću indukcije i Rb. Translokacija.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta Veterinaria-Beograd
T1  - Frequency of chromosomal aberrations in the evaluation of genotoxic potential of dicyclohexylamine (fumagillin) in vivo
T1  - Učestalost hromozomskih aberacija u funkciji evaluacije genotoksičnog potencijala fumagilina in vivo
VL  - 56
IS  - 4
SP  - 353
EP  - 366
DO  - 10.2298/AVB0604353S
ER  - 

@article{
author = "Stanimirović, Zoran and Stevanović, Jevrosima and Kulić, Milan and Stojić, Velibor",
year = "2006",
abstract = "Dicyclohexylamine (fumagillin), as an antibiotic produced by fermentation of Aspergillus fumigatus, is used in human medicine for the treatment of intestinal microsporidiosis in patients with HIV infection, intestinal amebiasis and microsporidial keratoconjunctivitis. In veterinary medicine fumagillin is effective in suppressing microsporidiosis of bees and fish. In this study, the genotoxicity of fumagillin was evaluated in mouse bone marrow cells using chromosome aberrations (CA) assay. Dicyclohexylamine was administered to mice by gavage in a dose of 25, 50, 75 mg/kg b.w., with water-sugar syrup as the negative control and cyclophosphamide as the positive control (40 mg/kg b.w) Significantly increased frequency (p lt 0.01 or p lt 0.001) of numerical chromosomal aberrations (aneupliodies and polyploidies) and structural chromosomal aberrations of gaps, breaks and centric rings were observed only at the highest experimental dose of dicyclohexylamine, compared with the negative control. However, in point of induction of Rb translocations, both the median (50 mg/kg b.w) and highest (75 mg/kg b.w) experimental dose showed a significant (p lt 00.001) increase (7.12 ± 0.26 and 9.00 ± 0.10, respectively) in comparison with the negative control (0.00 ± 0.00). Chromosomes 4 and 19 participated in these Rb translocations. These results suggest that dicyclohexilamine (fumagillin) has genotoxic potential in mammal in vivo chromosomal aberration (CA) test system., Dicyclohexylamin (fumagilin), kao antibiotik koga proizvodi gljivica Aspergillus fumigatus, se koristi u humanoj medicini u borbi protiv intestinalnih mikrosporidioza kod pacijenata sa HIV infekcijom, ali i za tretman obolelih od intestinalnih amebijaza i mikrosporidijanog keratokonjunktivitisa. U veterinarskoj medicini efikasan je u lečenju mikrosporidioza pčela i drugih insekata uzrokovanih sa Nosema apis, N. cerani, N. bombici, ali i mikrosporidioza riba uzrokovanih sa Myxobolus cerebralis i Tetracapsuloides bryosalmonae. U ovom radu ispitivana je genotoksičnost dicikloheksilamina kvantifikacijom i kvalifikacijom hromozomskih aberacija u ćelijama kostne srži miševa soja BALB/c. Dicikloheksilamin je aplikovan miševima intragastrično u dozama od 25, 50, 75 mg/kg b.w., pri čemu je vodeno-šećerni sirup predstavljao negativnu, a cyclophosphamid (40 mg/kg b.w) poozitivnu kontrolu. Utvrđeno je da samo najveća doza dicikloheksilamina signifikantno povećava učestalost (p lt 0.01 ili p lt 0.001) numeričkih hromozomskih aberacija (aneupliodija i poliploidija) i strukturnih hromozomskih aberacija tipa gapova, prekida i centričnih ringova u poređenju sa negativnom kontrolom. Međutim, srednja (50 mg/kg b.w) i najviša (75 mg/kg b.w) eksperimentalna doza izazvale su signifikantno (p lt 0.001) povećanje (7.12 ± 0.26 i 9.00 ± 0.10, respektivno) Robetrsonovih (Rb) translokacija u poređenju sa negativnom kontrolom (0.00 ± 0.00). U formiranju ovih Rb translokacija utvrđeno je da učestvuju hromozomi 4 i 19. Ovi rezultati sugerišu da je dicikloheksilamin (fumagilin) ispoljio visok genotoksični potencijal sa sposobnošću indukcije i Rb. Translokacija.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta Veterinaria-Beograd",
title = "Frequency of chromosomal aberrations in the evaluation of genotoxic potential of dicyclohexylamine (fumagillin) in vivo, Učestalost hromozomskih aberacija u funkciji evaluacije genotoksičnog potencijala fumagilina in vivo",
volume = "56",
number = "4",
pages = "353-366",
doi = "10.2298/AVB0604353S"
}

Stanimirović, Z., Stevanović, J., Kulić, M.,& Stojić, V.. (2006). Frequency of chromosomal aberrations in the evaluation of genotoxic potential of dicyclohexylamine (fumagillin) in vivo. in Acta Veterinaria-Beograd
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 56(4), 353-366.
https://doi.org/10.2298/AVB0604353S

Stanimirović Z, Stevanović J, Kulić M, Stojić V. Frequency of chromosomal aberrations in the evaluation of genotoxic potential of dicyclohexylamine (fumagillin) in vivo. in Acta Veterinaria-Beograd. 2006;56(4):353-366.
doi:10.2298/AVB0604353S .

Stanimirović, Zoran, Stevanović, Jevrosima, Kulić, Milan, Stojić, Velibor, "Frequency of chromosomal aberrations in the evaluation of genotoxic potential of dicyclohexylamine (fumagillin) in vivo" in Acta Veterinaria-Beograd, 56, no. 4 (2006):353-366,
https://doi.org/10.2298/AVB0604353S . .