TY  - JOUR
AU  - Plecas-Solarović, Bosiljka
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
PY  - 2014
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/1101
AB  - Alzheimers disease (AD) is the most frequent progressive neurodegenerative disorder in elderly associated with irreversible cognitive impairment and dementia. The vast majority of AD patients are sporadic (SAD) in which the disease develops after age of 65. Despite of century of research, we lack understanding of the SAD etiology and pathogenesis. Several hypotheses try to explain the main causes of brain degeneration in SAD, one of them assuming that genomic instability and the reentry of certain neurons into the incomplete cell cycle may be the pathogenic basis of the disease. Although the brain is the most affected organ in AD, numerous studies showed structural and functional alterations in peripheral tissues, suggesting that AD is a generalized systemic disorder. Diverse changes in peripheral cells from AD patients are described in literature including cell cycle aberration and chromosome instability, alterations in cell viability, proliferation and apoptosis, oxidative metabolism, amyloid precursor protein and amyloid beta protein metabolism, and other cellular processes. The aim of this paper was to summarize and review the results of our investigations and the growing literature data concerning the multiple chromosomal alterations in peripheral cells of AD patients and to consider their possible role in the disease pathogenesis as well as the importance of such investigations.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika
T1  - Cytogenetic alterations in peripheral cells of Alzheimer s disease patients
VL  - 46
IS  - 1
SP  - 315
EP  - 330
DO  - 10.2298/GENSR1401315P
ER  - 

@article{
author = "Plecas-Solarović, Bosiljka and Đelić, Ninoslav and Bajić, Vladan and Živković, Lada and Spremo-Potparević, Biljana",
year = "2014",
abstract = "Alzheimers disease (AD) is the most frequent progressive neurodegenerative disorder in elderly associated with irreversible cognitive impairment and dementia. The vast majority of AD patients are sporadic (SAD) in which the disease develops after age of 65. Despite of century of research, we lack understanding of the SAD etiology and pathogenesis. Several hypotheses try to explain the main causes of brain degeneration in SAD, one of them assuming that genomic instability and the reentry of certain neurons into the incomplete cell cycle may be the pathogenic basis of the disease. Although the brain is the most affected organ in AD, numerous studies showed structural and functional alterations in peripheral tissues, suggesting that AD is a generalized systemic disorder. Diverse changes in peripheral cells from AD patients are described in literature including cell cycle aberration and chromosome instability, alterations in cell viability, proliferation and apoptosis, oxidative metabolism, amyloid precursor protein and amyloid beta protein metabolism, and other cellular processes. The aim of this paper was to summarize and review the results of our investigations and the growing literature data concerning the multiple chromosomal alterations in peripheral cells of AD patients and to consider their possible role in the disease pathogenesis as well as the importance of such investigations.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika",
title = "Cytogenetic alterations in peripheral cells of Alzheimer s disease patients",
volume = "46",
number = "1",
pages = "315-330",
doi = "10.2298/GENSR1401315P"
}

Plecas-Solarović, B., Đelić, N., Bajić, V., Živković, L.,& Spremo-Potparević, B.. (2014). Cytogenetic alterations in peripheral cells of Alzheimer s disease patients. in Genetika
Društvo genetičara Srbije, Beograd., 46(1), 315-330.
https://doi.org/10.2298/GENSR1401315P

Plecas-Solarović B, Đelić N, Bajić V, Živković L, Spremo-Potparević B. Cytogenetic alterations in peripheral cells of Alzheimer s disease patients. in Genetika. 2014;46(1):315-330.
doi:10.2298/GENSR1401315P .

Plecas-Solarović, Bosiljka, Đelić, Ninoslav, Bajić, Vladan, Živković, Lada, Spremo-Potparević, Biljana, "Cytogenetic alterations in peripheral cells of Alzheimer s disease patients" in Genetika, 46, no. 1 (2014):315-330,
https://doi.org/10.2298/GENSR1401315P . .

TY  - JOUR
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Plecas-Solarović, Bosiljka
AU  - Đelić, Ninoslav
AU  - Ocić, Gordana
AU  - Smiljković, Predrag
AU  - Siedlak, Sandra L.
AU  - Smith, Mark A.
AU  - Bajić, Vladan
PY  - 2010
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/728
AB  - Chromosomal alterations are a feature of both aging and Alzheimers disease (AD). This study examined if premature centromere division (PCD), a chromosomal instability indicator increased in AD, is correlated with aging or, instead, represents a de novo chromosomal alteration due to accelerating aging in AD. PCD in peripheral blood lymphocytes was determined in sporadic AD patients and gender and age-matched unaffected controls. Metaphase nuclei were analyzed for chromosomes showing PCD, X chromosomes with PCD (PCD,X), and acrocentric chromosomes showing PCD. AD patients, regardless of age, demonstrated increased PCD on any chromosome and PCD on acrocentric chromosomes in both genders, whereas an increase in frequency of PCD,X was expressed only in women. This cytogenetic analysis suggests that PCD is a feature of AD, rather than an epiphenomenon of chronological aging, and may be useful as a physiological biomarker that can be used for disease diagnosis.
PB  - Oxford Univ Press Inc, Cary
T2  - Journals of Gerontology Series A-Biological Sciences and Medical Sciences
T1  - Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer s Disease Patients: Relation to Gender and Age
VL  - 65
IS  - 12
SP  - 1269
EP  - 1274
DO  - 10.1093/gerona/glq148
ER  - 

@article{
author = "Živković, Lada and Spremo-Potparević, Biljana and Plecas-Solarović, Bosiljka and Đelić, Ninoslav and Ocić, Gordana and Smiljković, Predrag and Siedlak, Sandra L. and Smith, Mark A. and Bajić, Vladan",
year = "2010",
abstract = "Chromosomal alterations are a feature of both aging and Alzheimers disease (AD). This study examined if premature centromere division (PCD), a chromosomal instability indicator increased in AD, is correlated with aging or, instead, represents a de novo chromosomal alteration due to accelerating aging in AD. PCD in peripheral blood lymphocytes was determined in sporadic AD patients and gender and age-matched unaffected controls. Metaphase nuclei were analyzed for chromosomes showing PCD, X chromosomes with PCD (PCD,X), and acrocentric chromosomes showing PCD. AD patients, regardless of age, demonstrated increased PCD on any chromosome and PCD on acrocentric chromosomes in both genders, whereas an increase in frequency of PCD,X was expressed only in women. This cytogenetic analysis suggests that PCD is a feature of AD, rather than an epiphenomenon of chronological aging, and may be useful as a physiological biomarker that can be used for disease diagnosis.",
publisher = "Oxford Univ Press Inc, Cary",
journal = "Journals of Gerontology Series A-Biological Sciences and Medical Sciences",
title = "Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer s Disease Patients: Relation to Gender and Age",
volume = "65",
number = "12",
pages = "1269-1274",
doi = "10.1093/gerona/glq148"
}

Živković, L., Spremo-Potparević, B., Plecas-Solarović, B., Đelić, N., Ocić, G., Smiljković, P., Siedlak, S. L., Smith, M. A.,& Bajić, V.. (2010). Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer s Disease Patients: Relation to Gender and Age. in Journals of Gerontology Series A-Biological Sciences and Medical Sciences
Oxford Univ Press Inc, Cary., 65(12), 1269-1274.
https://doi.org/10.1093/gerona/glq148

Živković L, Spremo-Potparević B, Plecas-Solarović B, Đelić N, Ocić G, Smiljković P, Siedlak SL, Smith MA, Bajić V. Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer s Disease Patients: Relation to Gender and Age. in Journals of Gerontology Series A-Biological Sciences and Medical Sciences. 2010;65(12):1269-1274.
doi:10.1093/gerona/glq148 .

Živković, Lada, Spremo-Potparević, Biljana, Plecas-Solarović, Bosiljka, Đelić, Ninoslav, Ocić, Gordana, Smiljković, Predrag, Siedlak, Sandra L., Smith, Mark A., Bajić, Vladan, "Premature Centromere Division of Metaphase Chromosomes in Peripheral Blood Lymphocytes of Alzheimer s Disease Patients: Relation to Gender and Age" in Journals of Gerontology Series A-Biological Sciences and Medical Sciences, 65, no. 12 (2010):1269-1274,
https://doi.org/10.1093/gerona/glq148 . .

TY  - JOUR
AU  - Spremo-Potparević, Biljana
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Plecas-Solarović, Bosiljka
AU  - Smith, Mark A.
AU  - Bajić, Vladan
PY  - 2008
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/539
AB  - Premature centromere division (PCD) represents a loss of control over the sequential separation and segregation of chromosome centromeres. Although first described in aging women, PCD on the X chromosome (PCD,X) is markedly elevated in peripheral blood lymphocytes of individuals suffering from Alzheimer disease (AD). The present study evaluated PCD,X, using a fluorescent in situ hybridization method, in interphase nuclei of frontal cerebral cortex neurons from sporadic AD patients and age-matched controls. The average frequency of PCD,X in AD patients (8.60 +/- 1.20%) was almost three times higher (p < 0.01) than in the control group (2.96 +/- 1.20). However, consistent with previous studies, no mitotic cells were found in neurons in either AD or control brain, suggesting an intrinsic inability of post-mitotic neurons to divide. In view of the fact that it has been well-documented that neurons in AD can re-enter into the cell division cycle, the findings presented here of increased PCD advance the hypothesis that deregulation of the cell cycle may contribute to neuronal degeneration and subsequent cognitive deficits in AD.
PB  - Wiley-Blackwell, Malden
T2  - Journal of Neurochemistry
T1  - Premature centromere division of the X chromosome in neurons in Alzheimer s disease
VL  - 106
IS  - 5
SP  - 2218
EP  - 2223
DO  - 10.1111/j.1471-4159.2008.05555.x
ER  - 

@article{
author = "Spremo-Potparević, Biljana and Živković, Lada and Đelić, Ninoslav and Plecas-Solarović, Bosiljka and Smith, Mark A. and Bajić, Vladan",
year = "2008",
abstract = "Premature centromere division (PCD) represents a loss of control over the sequential separation and segregation of chromosome centromeres. Although first described in aging women, PCD on the X chromosome (PCD,X) is markedly elevated in peripheral blood lymphocytes of individuals suffering from Alzheimer disease (AD). The present study evaluated PCD,X, using a fluorescent in situ hybridization method, in interphase nuclei of frontal cerebral cortex neurons from sporadic AD patients and age-matched controls. The average frequency of PCD,X in AD patients (8.60 +/- 1.20%) was almost three times higher (p < 0.01) than in the control group (2.96 +/- 1.20). However, consistent with previous studies, no mitotic cells were found in neurons in either AD or control brain, suggesting an intrinsic inability of post-mitotic neurons to divide. In view of the fact that it has been well-documented that neurons in AD can re-enter into the cell division cycle, the findings presented here of increased PCD advance the hypothesis that deregulation of the cell cycle may contribute to neuronal degeneration and subsequent cognitive deficits in AD.",
publisher = "Wiley-Blackwell, Malden",
journal = "Journal of Neurochemistry",
title = "Premature centromere division of the X chromosome in neurons in Alzheimer s disease",
volume = "106",
number = "5",
pages = "2218-2223",
doi = "10.1111/j.1471-4159.2008.05555.x"
}

Spremo-Potparević, B., Živković, L., Đelić, N., Plecas-Solarović, B., Smith, M. A.,& Bajić, V.. (2008). Premature centromere division of the X chromosome in neurons in Alzheimer s disease. in Journal of Neurochemistry
Wiley-Blackwell, Malden., 106(5), 2218-2223.
https://doi.org/10.1111/j.1471-4159.2008.05555.x

Spremo-Potparević B, Živković L, Đelić N, Plecas-Solarović B, Smith MA, Bajić V. Premature centromere division of the X chromosome in neurons in Alzheimer s disease. in Journal of Neurochemistry. 2008;106(5):2218-2223.
doi:10.1111/j.1471-4159.2008.05555.x .

Spremo-Potparević, Biljana, Živković, Lada, Đelić, Ninoslav, Plecas-Solarović, Bosiljka, Smith, Mark A., Bajić, Vladan, "Premature centromere division of the X chromosome in neurons in Alzheimer s disease" in Journal of Neurochemistry, 106, no. 5 (2008):2218-2223,
https://doi.org/10.1111/j.1471-4159.2008.05555.x . .