TY  - JOUR
AU  - Đukanović, Đorđe
AU  - Gajić Bojić, Milica
AU  - Marinković, Sonja
AU  - Trailović, Saša
AU  - Stojiljkovic, Miloš P.
AU  - Škrbić, Ranko
PY  - 2022
UR  - https://vet-erinar.vet.bg.ac.rs/handle/123456789/2628
AB  - Carvacrol (CRV) is the main compound of essential oils extracted primarily from Thymus and Origanum species. Its various biological activities were confirmed: antioxidant, anti-inflammatory, antibacterial, antifungal, anti-tumour, antinematodal, and vasorelaxant action. Although vasodilation mediated by CRV was previously described, the exact mechanism of its action has not yet been established. Hence, the aim of this study was to investigate CRV vasoactivity on human umbilical arteries (HUA) and the different pathways involved in its mechanism of action using the tissue bath methodology. CRV caused a significant decrease in vascular tension of 5-HT-pre-contracted umbilical arteries, with EC50 of 442.13 ± 33.8 µmol/L (mean ± standard error of the mean—SEM). At 300 µmol/L, CRV shifted downward the 5-HT concentration–response curve with a statistical significance of p < 0.001 obtained for the four highest concentrations. At a concentration of 1 mmol/L, CRV completely abolished BaCl2-induced contraction in Ca2+-free Krebs–Ringer bicarbonate solution and the BAY K 8644-induced contraction in Krebs–Ringer bicarbonate solution (p < 0.001). Isopentenyl pyrophosphate, the antagonist of TRPV3 channel, was able to decrease the efficacy of CRV (p < 0.001). The blocking of L-type Ca2+ channels on smooth muscle cells is the most probable mechanism of CRV-induced vasorelaxation. However, the role of TRPV3 channels in CRV-induced vasodilation of HUA cannot be excluded either.
PB  - Canadian Science Publishing
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery
VL  - 100
SP  - 755
SP  - 762
DO  - 10.1139/cjpp-2021-0736
ER  - 

@article{
author = "Đukanović, Đorđe and Gajić Bojić, Milica and Marinković, Sonja and Trailović, Saša and Stojiljkovic, Miloš P. and Škrbić, Ranko",
year = "2022",
abstract = "Carvacrol (CRV) is the main compound of essential oils extracted primarily from Thymus and Origanum species. Its various biological activities were confirmed: antioxidant, anti-inflammatory, antibacterial, antifungal, anti-tumour, antinematodal, and vasorelaxant action. Although vasodilation mediated by CRV was previously described, the exact mechanism of its action has not yet been established. Hence, the aim of this study was to investigate CRV vasoactivity on human umbilical arteries (HUA) and the different pathways involved in its mechanism of action using the tissue bath methodology. CRV caused a significant decrease in vascular tension of 5-HT-pre-contracted umbilical arteries, with EC50 of 442.13 ± 33.8 µmol/L (mean ± standard error of the mean—SEM). At 300 µmol/L, CRV shifted downward the 5-HT concentration–response curve with a statistical significance of p < 0.001 obtained for the four highest concentrations. At a concentration of 1 mmol/L, CRV completely abolished BaCl2-induced contraction in Ca2+-free Krebs–Ringer bicarbonate solution and the BAY K 8644-induced contraction in Krebs–Ringer bicarbonate solution (p < 0.001). Isopentenyl pyrophosphate, the antagonist of TRPV3 channel, was able to decrease the efficacy of CRV (p < 0.001). The blocking of L-type Ca2+ channels on smooth muscle cells is the most probable mechanism of CRV-induced vasorelaxation. However, the role of TRPV3 channels in CRV-induced vasodilation of HUA cannot be excluded either.",
publisher = "Canadian Science Publishing",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery",
volume = "100",
pages = "755-762",
doi = "10.1139/cjpp-2021-0736"
}

Đukanović, Đ., Gajić Bojić, M., Marinković, S., Trailović, S., Stojiljkovic, M. P.,& Škrbić, R.. (2022). Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery. in Canadian Journal of Physiology and Pharmacology
Canadian Science Publishing., 100, 755.
https://doi.org/10.1139/cjpp-2021-0736

Đukanović Đ, Gajić Bojić M, Marinković S, Trailović S, Stojiljkovic MP, Škrbić R. Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery. in Canadian Journal of Physiology and Pharmacology. 2022;100:755.
doi:10.1139/cjpp-2021-0736 .

Đukanović, Đorđe, Gajić Bojić, Milica, Marinković, Sonja, Trailović, Saša, Stojiljkovic, Miloš P., Škrbić, Ranko, "Vasorelaxant effect of monoterpene carvacrol on isolated human umbilical artery" in Canadian Journal of Physiology and Pharmacology, 100 (2022):755,
https://doi.org/10.1139/cjpp-2021-0736 . .